CN110638054A - Method for extracting amino acid from dried nitraria tangutorum bobr - Google Patents
Method for extracting amino acid from dried nitraria tangutorum bobr Download PDFInfo
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- CN110638054A CN110638054A CN201910959751.9A CN201910959751A CN110638054A CN 110638054 A CN110638054 A CN 110638054A CN 201910959751 A CN201910959751 A CN 201910959751A CN 110638054 A CN110638054 A CN 110638054A
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- nitraria tangutorum
- tangutorum bobr
- amino acid
- extracting
- ethanol
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- 241001104824 Nitraria tangutorum Species 0.000 title claims abstract description 36
- 150000001413 amino acids Chemical class 0.000 title claims abstract description 19
- 238000000034 method Methods 0.000 title claims abstract description 18
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 35
- 238000000605 extraction Methods 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000000706 filtrate Substances 0.000 claims abstract description 8
- 238000001914 filtration Methods 0.000 claims abstract description 7
- 238000009210 therapy by ultrasound Methods 0.000 claims abstract description 6
- 238000002791 soaking Methods 0.000 claims abstract description 4
- 238000005406 washing Methods 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 239000002904 solvent Substances 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 4
- 239000004480 active ingredient Substances 0.000 abstract description 2
- 238000004821 distillation Methods 0.000 abstract description 2
- 239000000203 mixture Substances 0.000 abstract description 2
- 238000011085 pressure filtration Methods 0.000 abstract description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 abstract description 2
- 238000003828 vacuum filtration Methods 0.000 abstract description 2
- 239000000284 extract Substances 0.000 abstract 1
- 235000013399 edible fruits Nutrition 0.000 description 6
- 235000013175 Crataegus laevigata Nutrition 0.000 description 3
- 244000265913 Crataegus laevigata Species 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000004576 sand Substances 0.000 description 2
- 241000167854 Bourreria succulenta Species 0.000 description 1
- 240000000171 Crataegus monogyna Species 0.000 description 1
- 235000019750 Crude protein Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 208000037093 Menstruation Disturbances Diseases 0.000 description 1
- 206010027339 Menstruation irregular Diseases 0.000 description 1
- 241000159206 Nitraria Species 0.000 description 1
- 241001104795 Nitraria sibirica Species 0.000 description 1
- 208000033809 Suppuration Diseases 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 241000159213 Zygophyllaceae Species 0.000 description 1
- 206010000269 abscess Diseases 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 235000019693 cherries Nutrition 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 201000006549 dyspepsia Diseases 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002212 flavone derivatives Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 230000006651 lactation Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 238000002525 ultrasonication Methods 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/175—Amino acids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a method for extracting amino acid from nitraria tangutorum bobr, which is characterized in that nitraria tangutorum bobr is cleaned and dried in the air; placing the mixture into a pulverizer to be pulverized, adding a certain amount of water, and carrying out ultrasonic treatment for a period of time; adding a certain amount of ethanol, soaking, extracting with ethanol for a period of time, filtering, and collecting filtrate; and then carrying out vacuum filtration on the filtrate, and removing the solvent to obtain the amino acid. The invention uses alcohol extraction method and uses ultrasonic wave to act on the crushed nitraria tangutorum bobr, extracts effective components in cells through cavitation phenomenon, and improves the extraction rate of the effective components. The operation steps are simple and easy, and the process is pollution-free; by selecting the most appropriate temperature and time, the alcohol extraction efficiency is improved, the yield is improved, and the activity of the produced total amino is ensured; the reduced pressure filtration mode effectively prevents the influence of high temperature distillation solvent on active ingredients, provides a new direction for the comprehensive utilization and the deep development of the nitraria tangutorum bobr, and has strong practicability and wide applicability.
Description
Technical Field
The invention relates to an extraction method of amino acid, in particular to a method for extracting amino acid from dried nitraria tangutorum bobr.
Background
The nitraria tangutorum bobr belongs to the genus nitraria and the family Zygophyllaceae, and is a multi-branch saline-alkali-resistant droughty type short shrub. Nitraria sibirica pall is usually grown in salinization areas in desert regions and has the physiological characteristics of drought resistance, high temperature resistance, strong water retention capacity and the like. The white thorns are excellent sand-fixing shrub plants in the sand areas of China and are important research objects in wind and sand protection. The fruits are oval like soybean grains, are dark red or orange yellow after being ripened, can be directly eaten, are called desert cherries and are called third-class fruits.
The nitraria tangutorum bobr has rich resources and wide distribution, and is wild in the places of Zhangjiakou, Tianjin, Cangzhou and the like in the northwest desert area of China and the coastal areas of North China and northeast China. According to statistics, the nitraria tangutorum bobr basins are distributed in pollution-free areas with the altitude of more than 3000 m, 6-10 ten thousand tons of nitraria tangutorum bobr can be produced every year, and the method has great development value. There are records as early as in compendium of materia Medica: bai Ci has pungent, cold and nontoxic flavor, and can treat swelling and abscess, promote pus discharge, tonify kidney qi and replenish essence and marrow. But the people really began to pay attention to the nutritional value of the nitraria tangutorum bobr after the 90 s of the 20 th century. Researches show that the content of crude protein in the nitraria tangutorum bobr is 10.6-14.4%, the content of fat and fruit oil in pulp is 3.5%, the content of amino acid C also reaches 7.8-18.3%, more importantly, the nitraria tangutorum bobr also contains abundant nutrient substances such as protein, fat, sugar and the like, and the content of flavone is up to 1.1-1.9%.
The nitraria tangutorum bobr has good medicinal and edible values, is sour, sweet and delicious, has the effects of nourishing the stomach, tonifying the spleen, relieving exterior syndrome, soothing the nerves, nourishing yin, moistening the lung, promoting lactation and the like, and also has certain curative effects on weakness, irregular menstruation and dyspepsia.
Disclosure of Invention
In order to solve the defects of the prior art, the invention aims to provide a method for extracting amino acid from the dried nitraria tangutorum bobr with high efficiency and low cost.
In order to achieve the above object, the present invention adopts the following technical solutions:
a method for extracting amino acid from a dried nitraria tangutorum bobr comprises the following steps:
s1, cleaning and airing the nitraria tangutorum bobr;
s2, placing the cleaned dried nitraria tangutorum bobr into a pulverizer to be pulverized, adding a certain amount of water, and carrying out ultrasonic treatment for a period of time;
s3, adding a certain amount of ethanol, soaking, extracting with ethanol for a period of time, and filtering to obtain a filtrate;
s4, filtering the filtrate under reduced pressure, and removing the solvent to obtain the amino acid.
The cleaning in the step S1 includes washing with running water, and then washing with deionized water; and drying, including draining with gauze and drying.
And (5) carrying out ultrasonic treatment in the step S2, wherein the power is 90-120w, and the time is 1-2 h. Preferably, the power is 120w and the time is 1 h.
The ethanol in the step S3 is 15-25 times of the dry mass of the nitraria tangutorum bobr, and the concentration of the ethanol is 60 wt%. Preferably 20 times the dry mass of the nitraria tangutorum bobr.
The alcohol extraction in the step S3 is carried out at the temperature of 60-70 ℃ for 1.5-2 h. The temperature is preferably 65 ℃.
The invention has the advantages that:
the invention relates to a method for extracting amino acid from nitraria tangutorum bobr, which utilizes ultrasonic waves to act on crushed nitraria tangutorum bobr, and then adopts an alcohol extraction method to extract total amino acid from the nitraria tangutorum bobr; when ultrasonic waves act on the medium (the white thorn), the medium is torn into a plurality of small cavities, the small cavities are closed instantly, and instantaneous pressure of thousands of atmospheres, namely cavitation, is generated when the small cavities are closed. The cell wall is broken up and decomposed due to the extremely high pressure generated in the cavitation process, so that the solvent can penetrate into the cell, components in the cell are dissolved in the solvent, and the components are dissolved in the solvent under the action of ultrasonic waves, so that the extraction rate of effective components is improved.
The method adopts an alcohol extraction method, takes ethanol as an extracting solution to extract the amino acid in the nitraria tangutorum bobr, has simple and easy operation steps, no pollution in the process, low alcohol extraction temperature, short time, high efficiency and low cost, improves the yield, and ensures the activity of the obtained total amino; the reduced pressure filtration mode effectively prevents the influence of high temperature distillation solvent on active ingredients, provides a new direction for the comprehensive utilization and the deep development of the nitraria tangutorum bobr, and has strong practicability and wide applicability.
Detailed Description
The present invention will be described in detail with reference to the following embodiments.
A method for extracting vitamins from a nitraria tangutorum bobr dry fruit comprises the following steps: the white thorn fruit intervention treatment (S1) → ultrasonication (S2) → alcohol extraction (S3) → vacuum filtration under reduced pressure (S4) → finished product.
S1, washing the bur fruits with flowing water to remove floating dust, washing with deionized water, and draining with gauze to dry for later use;
s2, placing the cleaned dried nitraria tangutorum bobr into a pulverizer, pulverizing for 30-50min at the low temperature at the rotating speed of 2000-3500r/min, adding water with the same volume, and carrying out ultrasonic treatment for 1-2h at the power of 90-120w for later use;
s3, adding 60 wt% ethanol 15-25 times of the weight of the nitraria tangutorum bobr dry, soaking and extracting the mixture with ethanol at 60-70 ℃ for 1.5-2 h, and filtering to obtain filtrate; standby;
s4, filtering the filtrate obtained by alcohol extraction under reduced pressure, and removing the solvent to obtain the amino acid.
Examples 1 to 6
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It should be understood by those skilled in the art that the above embodiments do not limit the present invention in any way, and all technical solutions obtained by using equivalent alternatives or equivalent variations fall within the scope of the present invention.
Claims (5)
1. A method for extracting amino acid from a dried nitraria tangutorum bobr is characterized by comprising the following steps:
s1, cleaning and airing the dried nitraria tangutorum bobr;
s2, placing the cleaned dried nitraria tangutorum bobr into a pulverizer to be pulverized, adding a certain amount of water, and carrying out ultrasonic treatment for a period of time;
s3, adding a certain amount of ethanol, soaking, extracting with ethanol for a period of time, and filtering to obtain a filtrate;
s4, filtering the filtrate under reduced pressure to obtain the amino acid.
2. The method of claim 1, wherein the washing in step S1 comprises washing with running water and then with deionized water;
and drying, including draining with gauze and drying.
3. The method for extracting amino acid from the dried Nitraria tangutorum bobr according to claim 1, wherein the ultrasonic treatment in the step S2 is performed at a power of 90-120w for 1-2 h.
4. The method for extracting amino acid from the dried nitraria tangutorum bobr according to claim 1, wherein the ethanol in the step S3 is 15-25 times of the mass of the dried nitraria tangutorum bobr, and the concentration of the ethanol is 60 wt%.
5. The method for extracting amino acid from the dried nitraria tangutorum bobr according to claim 1, wherein the alcohol extraction in the step S3 is performed at 60-70 ℃ for 1.5-2 h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910959751.9A CN110638054A (en) | 2019-10-10 | 2019-10-10 | Method for extracting amino acid from dried nitraria tangutorum bobr |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910959751.9A CN110638054A (en) | 2019-10-10 | 2019-10-10 | Method for extracting amino acid from dried nitraria tangutorum bobr |
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| Publication Number | Publication Date |
|---|---|
| CN110638054A true CN110638054A (en) | 2020-01-03 |
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| CN201910959751.9A Pending CN110638054A (en) | 2019-10-10 | 2019-10-10 | Method for extracting amino acid from dried nitraria tangutorum bobr |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103054021A (en) * | 2012-12-31 | 2013-04-24 | 青海省轻工业研究所有限责任公司 | Method for extracting compound from nitraria tangutorum peel |
| CN105213442A (en) * | 2015-10-28 | 2016-01-06 | 青海尤尼克生物技术有限公司 | A kind of Fructus Nitraria schoberi extractive of general flavone and preparation method thereof |
| CN109892464A (en) * | 2019-03-19 | 2019-06-18 | 南京益唯森生物科技有限公司 | A kind of preparation method that white bur is dry |
-
2019
- 2019-10-10 CN CN201910959751.9A patent/CN110638054A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103054021A (en) * | 2012-12-31 | 2013-04-24 | 青海省轻工业研究所有限责任公司 | Method for extracting compound from nitraria tangutorum peel |
| CN105213442A (en) * | 2015-10-28 | 2016-01-06 | 青海尤尼克生物技术有限公司 | A kind of Fructus Nitraria schoberi extractive of general flavone and preparation method thereof |
| CN109892464A (en) * | 2019-03-19 | 2019-06-18 | 南京益唯森生物科技有限公司 | A kind of preparation method that white bur is dry |
Non-Patent Citations (3)
| Title |
|---|
| 王沛: "《中药制药工程原理与设备》", 31 August 2016, 中国中医药出版社 * |
| 邢世瑞: "《宁夏中药志》", 31 March 2006, 宁夏人民出版社 * |
| 郭力等: "《中药化学》", 31 August 2018, 中国医药科技出版社 * |
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Application publication date: 20200103 |