CN103054021A - Method for extracting compound from nitraria tangutorum peel - Google Patents
Method for extracting compound from nitraria tangutorum peel Download PDFInfo
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- CN103054021A CN103054021A CN201210590574XA CN201210590574A CN103054021A CN 103054021 A CN103054021 A CN 103054021A CN 201210590574X A CN201210590574X A CN 201210590574XA CN 201210590574 A CN201210590574 A CN 201210590574A CN 103054021 A CN103054021 A CN 103054021A
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Abstract
The invention relates to the field of biological technology, in particular to a method for extracting a compound from nitraria tangutorum peel. The method comprises the steps of (1) cleaning, (2) degreasing, (3) extracting, (4) enzymolysis and filtering, (5) concentrating and drying, and (6) detecting. The method comprises the steps such as pre-treatment of raw materials, alcohol extraction, enzymolysis, millipore filtration, vacuum concentration, freeze drying, and detection, and has the characteristics such as easiness in extraction, safety and high efficiency. The method provides one set of complete technical scheme for extracting the compound from the nitraria tangutorum peel, has the advantages of safety and easiness in extraction, improvement of product added value and the like, and provides the precondition to the large-scale production of the nitraria tangutorum peel to a large extent.
Description
Technical field
The present invention relates to biological technical field, specifically relate to a kind of method of from white burbark, extracting compound.
Background technology
The wild fruit of white thorn is called as " the 3rd class fruit ", has huge economic utilization potential value.White thorn resource is abundant in China's output, widely distributed, and relatively concentrates distribution in the area, Qaidam in Qinghai, and condition of utilization is superior.The white thorn resource 150 that Qaidam area distributes ~ 2,000,000 mu, relatively concentrating distributes has 500,000 mu of Bai Cilinyue that exploitation is worth, and pericarp 1.5 ~ 2.5 ten thousand ton can be produced in annual institute berry of nitraria tangutorum bobr amount 6 ~ 100,000 of tying ton, and stock number is very abundant, demands urgently developing.Nutritional labeling in white bur and the pericarp thereof is unique, and wherein nutritional labeling and medicines and health protection active component kind are many, rich content.According to the study, contain abundant vitamin, binary and ternary hydroxy acid, amino acid (18 kinds more than, wherein 8 kinds of essential amino acids content are abundant), pectin, protopectin, flavonol, anthocyanin in berry of nitraria tangutorum bobr, fruit juice, the pericarp, spend glucoside in vain, polysaccharide, terpene, peptide, steroid, alkaloid and abundant tartaric acid, protein, various trace elements etc.
Summary of the invention
The purpose of this invention is to provide and a kind ofly extract the method for from white burbark extracting compound of compound as raw material through biotechnology with white burbark.
A kind of method of extracting compound from white burbark of the present invention is achieved by following technical proposals: a kind of method of extracting compound from white burbark of the present invention comprises the steps:
(1) clean: white burbark cleans to remove the impurity such as silt with circulating water;
(2) degreasing: because white burbark contains certain lipid material, affect extraction effect, want the dialogue burbark to carry out ungrease treatment before therefore extracting, remove lipid material; Degreasing adopts ether or benzinum organic solvent to carry out degreasing;
(3) extract: the white burbark low temperature drying that degreasing is complete, 90 ℃, press the solid-liquid ratio of 1:20, merging filtrate is extracted in the alcohol reflux extraction with 50% 5 hours altogether 3 times;
(4) enzymolysis, filtration: 0.4% of the adding total amount pectase in extract 45 ℃ of lower insulations 30 minutes, is warming up to 80 ℃ of enzymes that go out; Through filtering with microporous membrane, elimination impurity;
(5) concentrated, dry: as extract to be carried out Vacuum Concentration under 0.07mpa vacuum pressure, 40 ℃, be condensed into medicinal extract;
(6) detect: the mensuration of polysaccharide and general flavone is undertaken by calibration curve method; The mensuration of gross protein is undertaken by the method for GB5009.5-2010; The detection of total amino acid is undertaken by the method for GB/T 5009.124-2003; Total V
CMensuration undertaken by 2.6-dichloroindophenol method; The mensuration of OPC sulfuric acid-vanillin method.
A kind of method of extracting compound from white burbark of the present invention has following beneficial effect compared with prior art: the compound that the present invention extracts through biotechnology as raw material with white burbark, have outside the characteristics of similar other extract, because extracting, the pericarp that utilizes the wild berry forms, kept the active component in the pericarp, have simultaneously health care, added value is high.As contain abundant amino acid, vitamin and other active component.The inventive method is take white burbark as raw material, adopts the active ingredient in the alcohol extracting method extraction pericarp, has kept to greatest extent the stability of active ingredient in the white burbark, has the characteristics such as safe, convenient, nontoxic.This invention has broken through the present situation of extracting merely the low value-added products such as pigment, processing feed with white burbark for intensive processing, the comprehensive utilization of white burbark provide a cover perfect technical scheme.Adopt that main component is polysaccharide 250mg/g, total amino acid 27.5mg/g, gross protein 100mg/ml, total V in the compound that this method extracts
C15.14mg/100g, general flavone 28.1mg/g, OPC 9.13 mg/g.
A kind of method of extracting compound from white burbark of the present invention comprises the steps such as raw material pre-treatment, alcohol extracting, enzymolysis, miillpore filter, Vacuum Concentration, freeze drying and detection, has the characteristics such as easy, safe and efficient of extracting.The present invention provides a cover perfect technical scheme for extract compound from white burbark, has the advantages such as extraction is safe and simple, raising added value of product, and the large-scale production of dialogue burbark provides prerequisite to a great extent.
Description of drawings
Fig. 1 is a kind of method process flow diagram that extracts compound from white burbark of the present invention.
The specific embodiment
Below in conjunction with drawings and Examples a kind of method and technology scheme of extracting compound from white burbark of the present invention is further described.
As shown in Figure 1, a kind of method of extracting compound from white burbark comprises the steps:
(1) clean: white burbark cleans to remove the impurity such as silt with circulating water;
(2) degreasing: because white burbark contains certain lipid material, affect extraction effect, want the dialogue burbark to carry out ungrease treatment before therefore extracting, remove lipid material; Degreasing adopts ether or benzinum organic solvent to carry out degreasing;
(3) extract: the white burbark low temperature drying that degreasing is complete, 90 ℃, press the solid-liquid ratio of 1:20, merging filtrate is extracted in the alcohol reflux extraction with 50% 5 hours altogether 3 times;
(4) enzymolysis, filtration: 0.4% of the adding total amount pectase in extract 45 ℃ of lower insulations 30 minutes, is warming up to 80 ℃ of enzymes that go out; Through filtering with microporous membrane, elimination impurity;
(5) concentrated, dry: as extract to be carried out Vacuum Concentration under 0.07mpa vacuum pressure, 40 ℃, be condensed into medicinal extract;
(6) detect: the mensuration of polysaccharide and general flavone is undertaken by calibration curve method; The mensuration of gross protein is undertaken by the method for GB5009.5-2010; The detection of total amino acid is undertaken by the method for GB/T 5009.124-2003; Total V
CMensuration undertaken by 2.6-dichloroindophenol method; The mensuration of OPC sulfuric acid-vanillin method.
Described (5) concentrated, drying steps is condensed in the medicinal extract postlyophilization case low temperature drying and makes powder, and the freeze drying temperature is-5 ℃, cooling time 36 hours.
Embodiment 1.
A kind of method of extracting compound from white burbark comprises the steps:
(1) clean: white burbark cleans to remove the impurity such as silt with circulating water;
(2) degreasing: because white burbark contains certain lipid material, affect extraction effect, want the dialogue burbark to carry out ungrease treatment before therefore extracting, remove lipid material; Degreasing adopts ether or benzinum organic solvent to carry out degreasing;
(3) extract: the white burbark low temperature drying that degreasing is complete, 90 ℃, press the solid-liquid ratio of 1:20, merging filtrate is extracted in the alcohol reflux extraction with 50% 5 hours altogether 3 times;
(4) enzymolysis, filtration: 0.4% of the adding total amount pectase in extract 45 ℃ of lower insulations 30 minutes, is warming up to 80 ℃ of enzymes that go out; Through filtering with microporous membrane, elimination impurity;
(5) concentrated, dry: as extract to be carried out Vacuum Concentration under 0.07mpa vacuum pressure, 40 ℃, be condensed into medicinal extract;
(6) detect: the mensuration of polysaccharide and general flavone is undertaken by calibration curve method; The mensuration of gross protein is undertaken by the method for GB5009.5-2010; The detection of total amino acid is undertaken by the method for GB/T 5009.124-2003; Total V
CMensuration undertaken by 2.6-dichloroindophenol method; The mensuration of OPC sulfuric acid-vanillin method.
Embodiment 2.
A kind of method of extracting compound from white burbark comprises the steps:
(1) clean: white burbark cleans to remove the impurity such as silt with circulating water;
(2) degreasing: because white burbark contains certain lipid material, affect extraction effect, want the dialogue burbark to carry out ungrease treatment before therefore extracting, remove lipid material; Degreasing adopts ether or benzinum organic solvent to carry out degreasing;
(3) extract: the white burbark low temperature drying that degreasing is complete, 90 ℃, press the solid-liquid ratio of 1:20, merging filtrate is extracted in the alcohol reflux extraction with 50% 5 hours altogether 3 times;
(4) enzymolysis, filtration: 0.4% of the adding total amount pectase in extract 45 ℃ of lower insulations 30 minutes, is warming up to 80 ℃ of enzymes that go out; Through filtering with microporous membrane, elimination impurity;
(5) concentrated, dry: as extract to be carried out Vacuum Concentration under 0.07mpa vacuum pressure, 40 ℃, be condensed into medicinal extract; Be condensed in the medicinal extract postlyophilization case low temperature drying and make powder; The freeze drying temperature is-5 ℃, cooling time 36 hours;
(6) detect: the mensuration of polysaccharide and general flavone is undertaken by calibration curve method; The mensuration of gross protein is undertaken by the method for GB5009.5-2010; The detection of total amino acid is undertaken by the method for GB/T 5009.124-2003; Total V
CMensuration undertaken by 2.6-dichloroindophenol method; The mensuration of OPC sulfuric acid-vanillin method.
Claims (2)
1. a method of extracting compound from white burbark is characterized in that described method comprises the steps:
(1) clean: white burbark cleans to remove the impurity such as silt with circulating water;
(2) degreasing: because white burbark contains certain lipid material, affect extraction effect, want the dialogue burbark to carry out ungrease treatment before therefore extracting, remove lipid material; Degreasing adopts ether or benzinum organic solvent to carry out degreasing;
(3) extract: the white burbark low temperature drying that degreasing is complete, 90 ℃, press the solid-liquid ratio of 1:20, merging filtrate is extracted in the alcohol reflux extraction with 50% 5 hours altogether 3 times;
(4) enzymolysis, filtration: 0.4% of the adding total amount pectase in extract 45 ℃ of lower insulations 30 minutes, is warming up to 80 ℃ of enzymes that go out; Through filtering with microporous membrane, elimination impurity;
(5) concentrated, dry: as extract to be carried out Vacuum Concentration under 0.07mpa vacuum pressure, 40 ℃, be condensed into medicinal extract;
(6) detect: the mensuration of polysaccharide and general flavone is undertaken by calibration curve method; The mensuration of gross protein is undertaken by the method for GB5009.5-2010; The detection of total amino acid is undertaken by the method for GB/T 5009.124-2003; Total V
CMensuration undertaken by 2.6-dichloroindophenol method; The mensuration of OPC sulfuric acid-vanillin method.
2. the method for from white burbark, extracting compound according to claim 1, it is characterized in that: described (5) concentrated, drying steps is condensed in the medicinal extract postlyophilization case low temperature drying and makes powder, the freeze drying temperature is-35 ℃, cooling time 36 hours.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104262231A (en) * | 2014-09-29 | 2015-01-07 | 中国科学院西北高原生物研究所 | Method for extracting and separating L-tryptophan from nitraria tangutorum bobr seeds |
| CN104855850A (en) * | 2015-04-28 | 2015-08-26 | 青海省轻工业研究所有限责任公司 | Nitraria tangutorum skin residue extract with anti-fatigue effect and preparation method thereof |
| CN104987428A (en) * | 2015-07-23 | 2015-10-21 | 中国科学院西北高原生物研究所 | Compound enzyme-microwave extraction method for optimizing Nitraria polysaccharide by response surface methodology |
| CN107212381A (en) * | 2017-04-20 | 2017-09-29 | 中国科学院西北高原生物研究所 | A kind of preparation method of white bur ferment |
| CN110638054A (en) * | 2019-10-10 | 2020-01-03 | 南京益唯森生物科技有限公司 | Method for extracting amino acid from dried nitraria tangutorum bobr |
| CN110746517A (en) * | 2019-12-06 | 2020-02-04 | 中国科学院新疆理化技术研究所 | Method for optimizing ultrasonic-assisted extraction of Siberian nitraria polysaccharide based on response surface method and application |
| CN110772546A (en) * | 2019-10-10 | 2020-02-11 | 南京益唯森生物科技有限公司 | Method for extracting amino acid from nitraria tangutorum bobr pulp |
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| CN102060829A (en) * | 2010-11-29 | 2011-05-18 | 中国科学院西北高原生物研究所 | Method for extracting anthocyanin from nitratia seeds |
| CN102114061A (en) * | 2011-03-01 | 2011-07-06 | 青海省轻工业研究所有限责任公司 | Method for extracting polyphenols from Siberian nitraria peels |
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2012
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| CN102060829A (en) * | 2010-11-29 | 2011-05-18 | 中国科学院西北高原生物研究所 | Method for extracting anthocyanin from nitratia seeds |
| CN102114061A (en) * | 2011-03-01 | 2011-07-06 | 青海省轻工业研究所有限责任公司 | Method for extracting polyphenols from Siberian nitraria peels |
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| 和彦苓等: "内蒙古地产白刺果种氨基酸和维生素含量分析", 《包头医学院学报》, vol. 22, no. 4, 31 December 2006 (2006-12-31) * |
| 宋永朋等: "白刺果皮中原花青素的提取工艺", 《食品研究与开发》, vol. 33, no. 8, 31 August 2012 (2012-08-31) * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104262231A (en) * | 2014-09-29 | 2015-01-07 | 中国科学院西北高原生物研究所 | Method for extracting and separating L-tryptophan from nitraria tangutorum bobr seeds |
| CN104855850A (en) * | 2015-04-28 | 2015-08-26 | 青海省轻工业研究所有限责任公司 | Nitraria tangutorum skin residue extract with anti-fatigue effect and preparation method thereof |
| CN104987428A (en) * | 2015-07-23 | 2015-10-21 | 中国科学院西北高原生物研究所 | Compound enzyme-microwave extraction method for optimizing Nitraria polysaccharide by response surface methodology |
| CN107212381A (en) * | 2017-04-20 | 2017-09-29 | 中国科学院西北高原生物研究所 | A kind of preparation method of white bur ferment |
| CN110638054A (en) * | 2019-10-10 | 2020-01-03 | 南京益唯森生物科技有限公司 | Method for extracting amino acid from dried nitraria tangutorum bobr |
| CN110772546A (en) * | 2019-10-10 | 2020-02-11 | 南京益唯森生物科技有限公司 | Method for extracting amino acid from nitraria tangutorum bobr pulp |
| CN110746517A (en) * | 2019-12-06 | 2020-02-04 | 中国科学院新疆理化技术研究所 | Method for optimizing ultrasonic-assisted extraction of Siberian nitraria polysaccharide based on response surface method and application |
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Application publication date: 20130424 |