CN102993154A - Method for extracting purple sweet potato anthocyanin - Google Patents
Method for extracting purple sweet potato anthocyanin Download PDFInfo
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- CN102993154A CN102993154A CN 201210401603 CN201210401603A CN102993154A CN 102993154 A CN102993154 A CN 102993154A CN 201210401603 CN201210401603 CN 201210401603 CN 201210401603 A CN201210401603 A CN 201210401603A CN 102993154 A CN102993154 A CN 102993154A
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Abstract
The invention discloses a method for extracting purple sweet potato anthocyanin, which is characterized by comprising the steps of: (1) by using purple sweet potato as a raw material, drying, crushing, soaking with ethanol water with pH of 2-5 and weight of 5-30 times of that of the raw material, extracting, and filtering extracts to obtain filtrate; (2) adsorbing filtrate by macroporous adsorption resin and eluting by distilled water after completing the adsorption operation; (3) concentrating the collected eluant respectively until the solid content is from 10% to 40%, wherein the concentrating temperature is 40-55 DEG C; and (4) performing drying and vacuum drying on concentrates at 55 DEG C to obtain the anthocyanin product. According to the invention, the extracted anthocyanin product has the advantages of high yield and purity, low solvent consumption, low cost and the like, and is easy to industrially produce.
Description
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Technical field
The invention belongs to the biological extraction technical field, relate to a kind of extracting method of purple potato anthocyanidin.
Background technology
Purple potato is again black potato, English name PurplePotato, and potato meat is purple to intense violet color.It also is rich in anthocyanidin except the nutritive ingredient with common Ipomoea batatas.
Anthocyanidin (Anthocyanidin) is the class water-soluble natural pigment that nature extensively exists, and belongs to flavonoid compound.The mother nucleus structure of anthocyanidin is the flavylium ion positively charged ion of muttering, and contains 8 conjugated double bonds and consists of the height conjugated systems, often is connected to form anthocyanogen with one or more glucosides by glycosidic link.The glycosyl of anthocyanogen and hydroxyl can also form with the acids such as coumaric acid the anthocyanogen of acylations by ester bond.Anthocyanidin is the disease preventing and treating found of present scientific circles, safeguards the most direct, the most effective, the safest free-radical scavengers of human health; anthocyanidin has small molecule structure; be that unique energy sees through the material that hemato encephalic barrier is removed free radical protection brain cell, can reduce microbiotic to some harm of human body simultaneously.Anthocyanidin all is widely used in fields such as healthcare products, medicine and makeup.
For the separation of anthocyanidin, disclosed report has much at present, comprises the method for extracting anthocyanidin from different plants, because purple potato is rich in anthocyanidin, and abundant raw material, therefore, purple potato becomes the focus of Recent study.
Summary of the invention
The object of the present invention is to provide a kind of extracting method of purple potato anthocyanidin.
Purpose of the present invention can be achieved through the following technical solutions:
A kind of extracting method of purple potato anthocyanidin, the method may further comprise the steps:
(1) take purple potato as raw material, dry, pulverizing is extracted as 2 ~ 5 aqueous ethanolic solution immersion take 5 ~ 30 times of raw material weights, pH, and extract obtains filtrate after filtration;
(2) described filtrate is adsorbed by macroporous adsorbent resin, adsorb the complete rear distilled water wash-out of using;
(3) elutriant of collecting being concentrated into respectively solid content is between 10% to 40%, and thickening temperature is 40 ℃ ~ 55 ℃;
(4) enriched material is carried out drying, vacuum-drying, temperature is 55 ℃, obtains the anthocyanidin product.
The volume fraction of the aqueous ethanolic solution of step of the present invention (1) is 80%, extracts 30 ℃ of temperature, 5 hours extraction times, extracts united extraction liquid three times.
The extracting mode of step of the present invention (1) is that condensing reflux extraction, cold soaking extraction, countercurrent extraction, seepage are extracted, a kind of in the microwave auxiliary extraction extracting mode.
That the macroporous adsorbent resin of step of the present invention (2) comprises is nonpolar, low-pole, polar resin.
It is that thickening temperature is 50 ℃ that the elutriant of step of the present invention (3) is concentrated into solid content.
The described drying of step of the present invention (4) is spraying drying.
Beneficial effect of the present invention: the present invention is take purple potato as raw material, carries out through solvent extraction, macroporous adsorbent resin that fractionation by adsorption, elutriant are concentrated, drying obtains the anthocyanidin product.The method has the advantage that technical process is simple, separation efficiency is high, production cost is low, be easy to industrialization.
Embodiment
The invention will be further described below in conjunction with embodiment.
Embodiment 1
A kind of extracting method of purple potato anthocyanidin, the method may further comprise the steps: (1) is take purple potato as raw material, dry, pulverizing, with 20 times of purple potato raw material weights, volume fractions be 80%, pH is that 3 aqueous ethanolic solution soaks and extracts, extract 30 ℃ of temperature, 5 hours extraction times, extract three times, united extraction liquid, extracting solution obtain filtrate after filtration; (2) described filtrate is adsorbed by macroporous adsorbent resin, adsorb the complete rear distilled water wash-out of using; (3) elutriant of collecting being concentrated into respectively solid content is 30%, and thickening temperature is 50 ℃; (4) enriched material is carried out drying, vacuum-drying, temperature is 55 ℃, obtains the anthocyanidin product.Product anthocyanidin productive rate is 6.8%, and its purity is 97.2% after measured.
Embodiment 2
A kind of extracting method of purple potato anthocyanidin, the method may further comprise the steps: (1) is take purple potato as raw material, dry, pulverizing, with 12 times of purple potato raw material weights, volume fractions be 80%, pH is that 5 aqueous ethanolic solution soaks and extracts, extract 30 ℃ of temperature, 5 hours extraction times, extract three times, united extraction liquid, extracting solution obtain filtrate after filtration; (2) described filtrate is adsorbed by macroporous adsorbent resin, adsorb the complete rear distilled water wash-out of using; (3) elutriant of collecting being concentrated into respectively solid content is 15%, and thickening temperature is 40 ℃; (4) enriched material is carried out drying, vacuum-drying, temperature is 55 ℃, obtains the anthocyanidin product.Product anthocyanidin productive rate is 7.5%, and its purity is 98.1% after measured.
Claims (6)
1. the extracting method of a purple potato anthocyanidin, the method may further comprise the steps:
(1) take purple potato as raw material, dry, pulverizing is extracted as 2 ~ 5 aqueous ethanolic solution immersion take 5 ~ 30 times of raw material weights, pH, and extract obtains filtrate after filtration;
(2) described filtrate is adsorbed by macroporous adsorbent resin, adsorb the complete rear distilled water wash-out of using;
(3) elutriant of collecting being concentrated into respectively solid content is between 10% to 40%, and thickening temperature is 40 ℃ ~ 55 ℃;
(4) enriched material is carried out drying, vacuum-drying, temperature is 55 ℃, obtains the anthocyanidin product.
2. the extracting method of purple potato anthocyanidin according to claim 1, it is characterized in that: the volume fraction of the aqueous ethanolic solution of step (1) is 80%, extracts 30 ℃ of temperature, 5 hours extraction times, extracts united extraction liquid three times.
3. the extracting method of purple potato anthocyanidin according to claim 1 is characterized in that: the extracting mode of step (1) is that condensing reflux extraction, cold soaking extraction, countercurrent extraction, seepage are extracted, a kind of in the microwave auxiliary extraction extracting mode.
4. the extracting method of purple potato anthocyanidin according to claim 1 is characterized in that: the macroporous adsorbent resin of step (2) comprises nonpolar, low-pole, polar resin.
5. the extracting method of purple potato anthocyanidin according to claim 1, it is characterized in that: it is that thickening temperature is 50 ℃ that the elutriant of step (3) is concentrated into solid content.
6. the extracting method of purple potato anthocyanidin according to claim 1, it is characterized in that: the described drying of step (4) is spraying drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210401603 CN102993154A (en) | 2012-10-22 | 2012-10-22 | Method for extracting purple sweet potato anthocyanin |
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| CN 201210401603 CN102993154A (en) | 2012-10-22 | 2012-10-22 | Method for extracting purple sweet potato anthocyanin |
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| CN102993154A true CN102993154A (en) | 2013-03-27 |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103450703A (en) * | 2013-09-25 | 2013-12-18 | 宁波海通食品科技有限公司 | Method for preparing high-purity taste-removing purple sweet potato pigment |
| CN103601712A (en) * | 2013-11-13 | 2014-02-26 | 王玉清 | Simple and convenient method for rapidly extracting anthocyanin from purple sweet potatoes |
| CN103880804A (en) * | 2014-03-20 | 2014-06-25 | 江苏大学 | Method for extracting and separating anthocyanin in purple potato powder |
| CN104926778A (en) * | 2015-06-03 | 2015-09-23 | 陈玲华 | Purple sweet potato anthocyanin extraction process |
| CN105380847A (en) * | 2015-11-25 | 2016-03-09 | 广州聚注专利研发有限公司 | Easily-cleaned purple eye shadow and preparation method thereof |
| CN105418573A (en) * | 2015-11-13 | 2016-03-23 | 大兴安岭林格贝寒带生物科技股份有限公司 | Industrialization method for extracting purple sweet potato anthocyanin from purple sweet potatoes |
| CN107125509A (en) * | 2017-06-15 | 2017-09-05 | 西南大学 | A kind of processing method of purple potato violet cabbage composite beverage |
| CN107232461A (en) * | 2017-06-15 | 2017-10-10 | 西南大学 | A kind of processing method of purple potato blueberry composite beverage |
| CN107382949A (en) * | 2017-08-29 | 2017-11-24 | 贵州省苗岭经济林技术开发有限公司 | A kind of purple potato wine brewing extraction purple sweet potato anthocyanin method |
-
2012
- 2012-10-22 CN CN 201210401603 patent/CN102993154A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103450703A (en) * | 2013-09-25 | 2013-12-18 | 宁波海通食品科技有限公司 | Method for preparing high-purity taste-removing purple sweet potato pigment |
| CN103601712A (en) * | 2013-11-13 | 2014-02-26 | 王玉清 | Simple and convenient method for rapidly extracting anthocyanin from purple sweet potatoes |
| CN103880804A (en) * | 2014-03-20 | 2014-06-25 | 江苏大学 | Method for extracting and separating anthocyanin in purple potato powder |
| CN103880804B (en) * | 2014-03-20 | 2015-12-02 | 江苏大学 | A kind of method of anthocyanidin in extraction and isolation purple sweet potato powder |
| CN104926778A (en) * | 2015-06-03 | 2015-09-23 | 陈玲华 | Purple sweet potato anthocyanin extraction process |
| CN105418573A (en) * | 2015-11-13 | 2016-03-23 | 大兴安岭林格贝寒带生物科技股份有限公司 | Industrialization method for extracting purple sweet potato anthocyanin from purple sweet potatoes |
| CN105418573B (en) * | 2015-11-13 | 2018-04-24 | 大兴安岭林格贝寒带生物科技股份有限公司 | The industrial method of purple sweet potato cyanidin is extracted from purple sweetpotato |
| CN105380847A (en) * | 2015-11-25 | 2016-03-09 | 广州聚注专利研发有限公司 | Easily-cleaned purple eye shadow and preparation method thereof |
| CN107125509A (en) * | 2017-06-15 | 2017-09-05 | 西南大学 | A kind of processing method of purple potato violet cabbage composite beverage |
| CN107232461A (en) * | 2017-06-15 | 2017-10-10 | 西南大学 | A kind of processing method of purple potato blueberry composite beverage |
| CN107382949A (en) * | 2017-08-29 | 2017-11-24 | 贵州省苗岭经济林技术开发有限公司 | A kind of purple potato wine brewing extraction purple sweet potato anthocyanin method |
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Application publication date: 20130327 |