CN102993154A - Method for extracting purple sweet potato anthocyanin - Google Patents
Method for extracting purple sweet potato anthocyanin Download PDFInfo
- Publication number
- CN102993154A CN102993154A CN 201210401603 CN201210401603A CN102993154A CN 102993154 A CN102993154 A CN 102993154A CN 201210401603 CN201210401603 CN 201210401603 CN 201210401603 A CN201210401603 A CN 201210401603A CN 102993154 A CN102993154 A CN 102993154A
- Authority
- CN
- China
- Prior art keywords
- purple potato
- extracting
- extraction
- drying
- anthocyanidin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 21
- 244000017020 Ipomoea batatas Species 0.000 title abstract description 4
- 235000002678 Ipomoea batatas Nutrition 0.000 title abstract description 4
- 229930002877 anthocyanin Natural products 0.000 title abstract 4
- 235000010208 anthocyanin Nutrition 0.000 title abstract 4
- 239000004410 anthocyanin Substances 0.000 title abstract 4
- 150000004636 anthocyanins Chemical class 0.000 title abstract 4
- 239000000284 extract Substances 0.000 claims abstract description 13
- 239000002994 raw material Substances 0.000 claims abstract description 12
- 238000001035 drying Methods 0.000 claims abstract description 11
- 239000000706 filtrate Substances 0.000 claims abstract description 10
- 239000000047 product Substances 0.000 claims abstract description 10
- 239000011347 resin Substances 0.000 claims abstract description 10
- 229920005989 resin Polymers 0.000 claims abstract description 10
- 239000007787 solid Substances 0.000 claims abstract description 7
- 239000012153 distilled water Substances 0.000 claims abstract description 5
- 238000001914 filtration Methods 0.000 claims abstract description 5
- 238000001291 vacuum drying Methods 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 5
- 238000002791 soaking Methods 0.000 claims abstract description 3
- 229930014669 anthocyanidin Natural products 0.000 claims description 29
- 150000001452 anthocyanidin derivatives Chemical class 0.000 claims description 29
- 235000008758 anthocyanidins Nutrition 0.000 claims description 29
- 235000004976 Solanum vernei Nutrition 0.000 claims description 23
- 241000352057 Solanum vernei Species 0.000 claims description 23
- 238000000605 extraction Methods 0.000 claims description 17
- 239000003463 adsorbent Substances 0.000 claims description 7
- 230000008719 thickening Effects 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 4
- 238000010298 pulverizing process Methods 0.000 claims description 4
- 238000007654 immersion Methods 0.000 claims description 2
- 238000010992 reflux Methods 0.000 claims description 2
- 238000005507 spraying Methods 0.000 claims description 2
- 238000001179 sorption measurement Methods 0.000 abstract description 3
- 230000008901 benefit Effects 0.000 abstract description 2
- 239000012141 concentrate Substances 0.000 abstract 1
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 abstract 1
- 239000002904 solvent Substances 0.000 abstract 1
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- -1 flavonoid compound Chemical class 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 229940123457 Free radical scavenger Drugs 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000010933 acylation Effects 0.000 description 1
- 238000005917 acylation reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000001218 blood-brain barrier Anatomy 0.000 description 1
- 210000004958 brain cell Anatomy 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 150000008131 glucosides Chemical class 0.000 description 1
- 125000003147 glycosyl group Chemical group 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 150000003384 small molecules Chemical group 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a method for extracting purple sweet potato anthocyanin, which is characterized by comprising the steps of: (1) by using purple sweet potato as a raw material, drying, crushing, soaking with ethanol water with pH of 2-5 and weight of 5-30 times of that of the raw material, extracting, and filtering extracts to obtain filtrate; (2) adsorbing filtrate by macroporous adsorption resin and eluting by distilled water after completing the adsorption operation; (3) concentrating the collected eluant respectively until the solid content is from 10% to 40%, wherein the concentrating temperature is 40-55 DEG C; and (4) performing drying and vacuum drying on concentrates at 55 DEG C to obtain the anthocyanin product. According to the invention, the extracted anthocyanin product has the advantages of high yield and purity, low solvent consumption, low cost and the like, and is easy to industrially produce.
Description
?
Technical field
The invention belongs to the biological extraction technical field, relate to a kind of extracting method of purple potato anthocyanidin.
Background technology
Purple potato is again black potato, English name PurplePotato, and potato meat is purple to intense violet color.It also is rich in anthocyanidin except the nutritive ingredient with common Ipomoea batatas.
Anthocyanidin (Anthocyanidin) is the class water-soluble natural pigment that nature extensively exists, and belongs to flavonoid compound.The mother nucleus structure of anthocyanidin is the flavylium ion positively charged ion of muttering, and contains 8 conjugated double bonds and consists of the height conjugated systems, often is connected to form anthocyanogen with one or more glucosides by glycosidic link.The glycosyl of anthocyanogen and hydroxyl can also form with the acids such as coumaric acid the anthocyanogen of acylations by ester bond.Anthocyanidin is the disease preventing and treating found of present scientific circles, safeguards the most direct, the most effective, the safest free-radical scavengers of human health; anthocyanidin has small molecule structure; be that unique energy sees through the material that hemato encephalic barrier is removed free radical protection brain cell, can reduce microbiotic to some harm of human body simultaneously.Anthocyanidin all is widely used in fields such as healthcare products, medicine and makeup.
For the separation of anthocyanidin, disclosed report has much at present, comprises the method for extracting anthocyanidin from different plants, because purple potato is rich in anthocyanidin, and abundant raw material, therefore, purple potato becomes the focus of Recent study.
Summary of the invention
The object of the present invention is to provide a kind of extracting method of purple potato anthocyanidin.
Purpose of the present invention can be achieved through the following technical solutions:
A kind of extracting method of purple potato anthocyanidin, the method may further comprise the steps:
(1) take purple potato as raw material, dry, pulverizing is extracted as 2 ~ 5 aqueous ethanolic solution immersion take 5 ~ 30 times of raw material weights, pH, and extract obtains filtrate after filtration;
(2) described filtrate is adsorbed by macroporous adsorbent resin, adsorb the complete rear distilled water wash-out of using;
(3) elutriant of collecting being concentrated into respectively solid content is between 10% to 40%, and thickening temperature is 40 ℃ ~ 55 ℃;
(4) enriched material is carried out drying, vacuum-drying, temperature is 55 ℃, obtains the anthocyanidin product.
The volume fraction of the aqueous ethanolic solution of step of the present invention (1) is 80%, extracts 30 ℃ of temperature, 5 hours extraction times, extracts united extraction liquid three times.
The extracting mode of step of the present invention (1) is that condensing reflux extraction, cold soaking extraction, countercurrent extraction, seepage are extracted, a kind of in the microwave auxiliary extraction extracting mode.
That the macroporous adsorbent resin of step of the present invention (2) comprises is nonpolar, low-pole, polar resin.
It is that thickening temperature is 50 ℃ that the elutriant of step of the present invention (3) is concentrated into solid content.
The described drying of step of the present invention (4) is spraying drying.
Beneficial effect of the present invention: the present invention is take purple potato as raw material, carries out through solvent extraction, macroporous adsorbent resin that fractionation by adsorption, elutriant are concentrated, drying obtains the anthocyanidin product.The method has the advantage that technical process is simple, separation efficiency is high, production cost is low, be easy to industrialization.
Embodiment
The invention will be further described below in conjunction with embodiment.
Embodiment 1
A kind of extracting method of purple potato anthocyanidin, the method may further comprise the steps: (1) is take purple potato as raw material, dry, pulverizing, with 20 times of purple potato raw material weights, volume fractions be 80%, pH is that 3 aqueous ethanolic solution soaks and extracts, extract 30 ℃ of temperature, 5 hours extraction times, extract three times, united extraction liquid, extracting solution obtain filtrate after filtration; (2) described filtrate is adsorbed by macroporous adsorbent resin, adsorb the complete rear distilled water wash-out of using; (3) elutriant of collecting being concentrated into respectively solid content is 30%, and thickening temperature is 50 ℃; (4) enriched material is carried out drying, vacuum-drying, temperature is 55 ℃, obtains the anthocyanidin product.Product anthocyanidin productive rate is 6.8%, and its purity is 97.2% after measured.
Embodiment 2
A kind of extracting method of purple potato anthocyanidin, the method may further comprise the steps: (1) is take purple potato as raw material, dry, pulverizing, with 12 times of purple potato raw material weights, volume fractions be 80%, pH is that 5 aqueous ethanolic solution soaks and extracts, extract 30 ℃ of temperature, 5 hours extraction times, extract three times, united extraction liquid, extracting solution obtain filtrate after filtration; (2) described filtrate is adsorbed by macroporous adsorbent resin, adsorb the complete rear distilled water wash-out of using; (3) elutriant of collecting being concentrated into respectively solid content is 15%, and thickening temperature is 40 ℃; (4) enriched material is carried out drying, vacuum-drying, temperature is 55 ℃, obtains the anthocyanidin product.Product anthocyanidin productive rate is 7.5%, and its purity is 98.1% after measured.
Claims (6)
1. the extracting method of a purple potato anthocyanidin, the method may further comprise the steps:
(1) take purple potato as raw material, dry, pulverizing is extracted as 2 ~ 5 aqueous ethanolic solution immersion take 5 ~ 30 times of raw material weights, pH, and extract obtains filtrate after filtration;
(2) described filtrate is adsorbed by macroporous adsorbent resin, adsorb the complete rear distilled water wash-out of using;
(3) elutriant of collecting being concentrated into respectively solid content is between 10% to 40%, and thickening temperature is 40 ℃ ~ 55 ℃;
(4) enriched material is carried out drying, vacuum-drying, temperature is 55 ℃, obtains the anthocyanidin product.
2. the extracting method of purple potato anthocyanidin according to claim 1, it is characterized in that: the volume fraction of the aqueous ethanolic solution of step (1) is 80%, extracts 30 ℃ of temperature, 5 hours extraction times, extracts united extraction liquid three times.
3. the extracting method of purple potato anthocyanidin according to claim 1 is characterized in that: the extracting mode of step (1) is that condensing reflux extraction, cold soaking extraction, countercurrent extraction, seepage are extracted, a kind of in the microwave auxiliary extraction extracting mode.
4. the extracting method of purple potato anthocyanidin according to claim 1 is characterized in that: the macroporous adsorbent resin of step (2) comprises nonpolar, low-pole, polar resin.
5. the extracting method of purple potato anthocyanidin according to claim 1, it is characterized in that: it is that thickening temperature is 50 ℃ that the elutriant of step (3) is concentrated into solid content.
6. the extracting method of purple potato anthocyanidin according to claim 1, it is characterized in that: the described drying of step (4) is spraying drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210401603 CN102993154A (en) | 2012-10-22 | 2012-10-22 | Method for extracting purple sweet potato anthocyanin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210401603 CN102993154A (en) | 2012-10-22 | 2012-10-22 | Method for extracting purple sweet potato anthocyanin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102993154A true CN102993154A (en) | 2013-03-27 |
Family
ID=47922325
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201210401603 Pending CN102993154A (en) | 2012-10-22 | 2012-10-22 | Method for extracting purple sweet potato anthocyanin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102993154A (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103450703A (en) * | 2013-09-25 | 2013-12-18 | 宁波海通食品科技有限公司 | Method for preparing high-purity taste-removing purple sweet potato pigment |
| CN103601712A (en) * | 2013-11-13 | 2014-02-26 | 王玉清 | Simple and convenient method for rapidly extracting anthocyanin from purple sweet potatoes |
| CN103880804A (en) * | 2014-03-20 | 2014-06-25 | 江苏大学 | Method for extracting and separating anthocyanin in purple potato powder |
| CN104926778A (en) * | 2015-06-03 | 2015-09-23 | 陈玲华 | Purple sweet potato anthocyanin extraction process |
| CN105380847A (en) * | 2015-11-25 | 2016-03-09 | 广州聚注专利研发有限公司 | Easily-cleaned purple eye shadow and preparation method thereof |
| CN105418573A (en) * | 2015-11-13 | 2016-03-23 | 大兴安岭林格贝寒带生物科技股份有限公司 | Industrialization method for extracting purple sweet potato anthocyanin from purple sweet potatoes |
| CN107125509A (en) * | 2017-06-15 | 2017-09-05 | 西南大学 | A kind of processing method of purple potato violet cabbage composite beverage |
| CN107232461A (en) * | 2017-06-15 | 2017-10-10 | 西南大学 | A kind of processing method of purple potato blueberry composite beverage |
| CN107382949A (en) * | 2017-08-29 | 2017-11-24 | 贵州省苗岭经济林技术开发有限公司 | A kind of purple potato wine brewing extraction purple sweet potato anthocyanin method |
-
2012
- 2012-10-22 CN CN 201210401603 patent/CN102993154A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103450703A (en) * | 2013-09-25 | 2013-12-18 | 宁波海通食品科技有限公司 | Method for preparing high-purity taste-removing purple sweet potato pigment |
| CN103601712A (en) * | 2013-11-13 | 2014-02-26 | 王玉清 | Simple and convenient method for rapidly extracting anthocyanin from purple sweet potatoes |
| CN103880804A (en) * | 2014-03-20 | 2014-06-25 | 江苏大学 | Method for extracting and separating anthocyanin in purple potato powder |
| CN103880804B (en) * | 2014-03-20 | 2015-12-02 | 江苏大学 | A kind of method of anthocyanidin in extraction and isolation purple sweet potato powder |
| CN104926778A (en) * | 2015-06-03 | 2015-09-23 | 陈玲华 | Purple sweet potato anthocyanin extraction process |
| CN105418573A (en) * | 2015-11-13 | 2016-03-23 | 大兴安岭林格贝寒带生物科技股份有限公司 | Industrialization method for extracting purple sweet potato anthocyanin from purple sweet potatoes |
| CN105418573B (en) * | 2015-11-13 | 2018-04-24 | 大兴安岭林格贝寒带生物科技股份有限公司 | The industrial method of purple sweet potato cyanidin is extracted from purple sweetpotato |
| CN105380847A (en) * | 2015-11-25 | 2016-03-09 | 广州聚注专利研发有限公司 | Easily-cleaned purple eye shadow and preparation method thereof |
| CN107125509A (en) * | 2017-06-15 | 2017-09-05 | 西南大学 | A kind of processing method of purple potato violet cabbage composite beverage |
| CN107232461A (en) * | 2017-06-15 | 2017-10-10 | 西南大学 | A kind of processing method of purple potato blueberry composite beverage |
| CN107382949A (en) * | 2017-08-29 | 2017-11-24 | 贵州省苗岭经济林技术开发有限公司 | A kind of purple potato wine brewing extraction purple sweet potato anthocyanin method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102993154A (en) | Method for extracting purple sweet potato anthocyanin | |
| CN104688801B (en) | A kind of complex enzyme combination ultrasound extracts the production technology of eucommia bark flavone from folium cortex eucommiae | |
| CN103420970A (en) | Method for extracting and purifying anthocyanidin | |
| CN104906153A (en) | Technological method for efficiently extracting ginkgo flavone | |
| CN103435588A (en) | Novel method for preparing blueberry anthocyanidin | |
| CN103012544B (en) | A kind of method extracting saponin and polysaccharide from tea seed grouts | |
| CN102850419A (en) | Method for extracting cordycepin and polysaccharide from cordyceps militaris | |
| CN103130762A (en) | Method for extracting anthocyanin from indigo indicum | |
| CN105294632A (en) | Industrial method for preparing lonicera caerulea fruit anthocyanidin from lonicera caerulea fruits | |
| CN107325138A (en) | A kind of method of the main anthocyanin of four kinds of extraction separation and purification in pomace from blackcurrant | |
| CN103432562A (en) | Method for extracting fresh ginger polyphenol from fresh ginger | |
| CN102453075A (en) | Separation and purification process of glycyrrhizic acid | |
| CN102579516A (en) | Method for separating purified seabuckthorn flavonoid from large berry seabuckthorn marc | |
| CN101134767A (en) | Highly effective abstraction method for preparation of high-purity ginko flavonoid | |
| CN106038821A (en) | Method for extracting total flavonoids from boxthorn leaves | |
| US20220031790A1 (en) | Method for producing red perilla leaf extract | |
| CN104974124A (en) | Method for extracting procyanidine from dahurian larch barks | |
| KR100817876B1 (en) | Separation of Proanthocyanidins from Pine Bark | |
| CN105503981B (en) | The method that violet cabbage anthocyanidin is extracted from violet cabbage | |
| CN104945450B (en) | A kind of method that Stibene-glucoside is extracted from the vine of multiflower knotweed | |
| CN101817884A (en) | Method for extracting narrow-leaved oleaster polysaccharide | |
| CN103833805A (en) | Process for refining glycyrrhizinic acid in liquorice | |
| CN102643261A (en) | Method for extracting cyanidin from blueberry wine residues | |
| CN105294631A (en) | Industrial method for preparing anthocyanidin from sambucus williamsii fruits | |
| CN101618052A (en) | Process for extracting total flavonoids from hippophae leaves |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130327 |