CN110637087A - 一个表观遗传操作植物表型可塑性性状的方法 - Google Patents
一个表观遗传操作植物表型可塑性性状的方法 Download PDFInfo
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Abstract
提供一个表观遗传操作植物表型可塑性性状的方法，通过简并密码子定量替换目的基因的可甲基化位点，通过设计合成基因，进行转基因操作；或利用CRISPR/Cas9技术构建植物表达载体进行原位在体基因简并密码子定量替换可甲基化位点，定量调控外源或原位在体目的基因甲基化程度和基因表达水平，达到定量调控目标植物的可塑性性状。通过定量调节ICE1基因的甲基化程度培育不同抗寒能力植物，为通过表观遗传技术培育抗寒作物提供了新的选择，为降低低温伤害对作物产量和品质等的影响提供了技术方法。
Description
PCT国内申请，说明书已公开。
Claims (14)
- PCT国内申请，权利要求书已公开。
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| CNPCT/CN2017/075976 | 2017-03-08 | ||
| PCT/CN2018/078295 WO2018161921A1 (zh) | 2017-03-08 | 2018-03-07 | 一个表观遗传操作植物表型可塑性性状的方法 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113980973A (zh) * | 2021-10-12 | 2022-01-28 | 贵州省烟草科学研究院 | 烟草磷吸收负调控基因NtSPX4及其应用 |
| CN114058629A (zh) * | 2021-11-11 | 2022-02-18 | 河南大学三亚研究院 | Zm00001d042263基因在调控玉米气孔发育中的应用 |
| CN114107367A (zh) * | 2021-11-29 | 2022-03-01 | 广西壮族自治区林业科学研究院 | 一种靶向切除尾叶桉cad基因编码区dna的基因编辑方法 |
| CN116530416A (zh) * | 2023-06-12 | 2023-08-04 | 中国热带农业科学院橡胶研究所 | 一种运用油菜素内酯促进橡胶树体胚发生的方法 |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110904257B (zh) * | 2019-11-08 | 2023-09-26 | 南京农业大学 | 一种水稻及杂草稻OsICE1基因启动子甲基化测定的特异性分子标记引物及其应用 |
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| CN116724890A (zh) * | 2023-06-15 | 2023-09-12 | 德兴市中医研究院试验培训基地 | 一种植物组培苗开放式培养的方法 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120227135A1 (en) * | 2011-03-02 | 2012-09-06 | Riken | Plant having enhanced resistance to environmental stress |
| CN103571853A (zh) * | 2012-08-08 | 2014-02-12 | 中国科学院亚热带农业生态研究所 | 密码子优化的Cry2Aa基因与重组载体以及改变作物抗性的方法 |
| CN104673803A (zh) * | 2013-11-29 | 2015-06-03 | 南京农业大学 | 基因甲基化在调控基因表达方面的应用 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102668903B (zh) * | 2012-05-09 | 2014-07-02 | 北京大学 | Dna甲基化修饰剂在调控棉花纤维长度中的应用 |
| US20200032288A1 (en) * | 2015-06-30 | 2020-01-30 | Stellenbosch University | Method of enhancing stress tolerance of monocotyledonous plants |
| CN105695481A (zh) * | 2016-03-26 | 2016-06-22 | 上海大学 | 水蜜桃果实ice1基因、其克隆方法及应用 |
-
2018
- 2018-03-07 WO PCT/CN2018/078295 patent/WO2018161921A1/zh active Search and Examination
- 2018-03-07 EP EP18764709.4A patent/EP3594349A4/en active Pending
- 2018-03-07 CN CN201880016443.6A patent/CN110637087B/zh active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120227135A1 (en) * | 2011-03-02 | 2012-09-06 | Riken | Plant having enhanced resistance to environmental stress |
| CN103571853A (zh) * | 2012-08-08 | 2014-02-12 | 中国科学院亚热带农业生态研究所 | 密码子优化的Cry2Aa基因与重组载体以及改变作物抗性的方法 |
| CN104673803A (zh) * | 2013-11-29 | 2015-06-03 | 南京农业大学 | 基因甲基化在调控基因表达方面的应用 |
Non-Patent Citations (4)
| Title |
|---|
| H J XIE 等: "ICE1 demethylation drives the range expansion of a plant invader through cold tolerance divergence", 《MOLECULAR ECOLOGY》 * |
| 张惠展: "《基因工程 第4版》", 31 January 2017, 上海：华东理工大学出版社 * |
| 翁绿水 等: "Cry2Aa基因优化设计及功能验证", 《农业生物技术学报》 * |
| 解洪杰: "外来植物紫茎泽兰通过ICE-CBFs转录途径的耐寒性分化向北入侵的分子机制研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113980973A (zh) * | 2021-10-12 | 2022-01-28 | 贵州省烟草科学研究院 | 烟草磷吸收负调控基因NtSPX4及其应用 |
| CN114058629A (zh) * | 2021-11-11 | 2022-02-18 | 河南大学三亚研究院 | Zm00001d042263基因在调控玉米气孔发育中的应用 |
| CN114058629B (zh) * | 2021-11-11 | 2022-12-30 | 河南大学三亚研究院 | Zm00001d042263基因在调控玉米气孔发育中的应用 |
| CN114107367A (zh) * | 2021-11-29 | 2022-03-01 | 广西壮族自治区林业科学研究院 | 一种靶向切除尾叶桉cad基因编码区dna的基因编辑方法 |
| CN116530416A (zh) * | 2023-06-12 | 2023-08-04 | 中国热带农业科学院橡胶研究所 | 一种运用油菜素内酯促进橡胶树体胚发生的方法 |
| CN116530416B (zh) * | 2023-06-12 | 2024-03-22 | 中国热带农业科学院橡胶研究所 | 一种运用油菜素内酯促进橡胶树体胚发生的方法 |
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| Publication number | Publication date |
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| CN110637087B (zh) | 2024-01-16 |
| WO2018161921A1 (zh) | 2018-09-13 |
| EP3594349A1 (en) | 2020-01-15 |
| EP3594349A4 (en) | 2020-11-11 |
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