CN110623670A - Split type respiration oxygen consumption real-time monitoring device - Google Patents
Split type respiration oxygen consumption real-time monitoring device Download PDFInfo
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- CN110623670A CN110623670A CN201910757213.1A CN201910757213A CN110623670A CN 110623670 A CN110623670 A CN 110623670A CN 201910757213 A CN201910757213 A CN 201910757213A CN 110623670 A CN110623670 A CN 110623670A
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- 230000036284 oxygen consumption Effects 0.000 title claims abstract description 170
- 238000012806 monitoring device Methods 0.000 title claims abstract description 33
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- 238000002474 experimental method Methods 0.000 claims description 19
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- 230000002950 deficient Effects 0.000 claims description 6
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 5
- 239000011521 glass Substances 0.000 claims description 5
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- 206010021143 Hypoxia Diseases 0.000 abstract description 17
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- WYTGDNHDOZPMIW-RCBQFDQVSA-N alstonine Natural products C1=CC2=C3C=CC=CC3=NC2=C2N1C[C@H]1[C@H](C)OC=C(C(=O)OC)[C@H]1C2 WYTGDNHDOZPMIW-RCBQFDQVSA-N 0.000 description 2
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/03—Housing for domestic or laboratory animals
- A01K1/031—Cages for laboratory animals; Cages for measuring metabolism of animals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K29/00—Other apparatus for animal husbandry
- A01K29/005—Monitoring or measuring activity, e.g. detecting heat or mating
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/08—Detecting, measuring or recording devices for evaluating the respiratory organs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/08—Detecting, measuring or recording devices for evaluating the respiratory organs
- A61B5/0816—Measuring devices for examining respiratory frequency
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/08—Detecting, measuring or recording devices for evaluating the respiratory organs
- A61B5/083—Measuring rate of metabolism by using breath test, e.g. measuring rate of oxygen consumption
- A61B5/0833—Measuring rate of oxygen consumption
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/103—Detecting, measuring or recording devices for testing the shape, pattern, colour, size or movement of the body or parts thereof, for diagnostic purposes
- A61B5/11—Measuring movement of the entire body or parts thereof, e.g. head or hand tremor, mobility of a limb
- A61B5/113—Measuring movement of the entire body or parts thereof, e.g. head or hand tremor, mobility of a limb occurring during breathing
- A61B5/1135—Measuring movement of the entire body or parts thereof, e.g. head or hand tremor, mobility of a limb occurring during breathing by monitoring thoracic expansion
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/40—Animals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/42—Evaluating a particular growth phase or type of persons or animals for laboratory research
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biophysics (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
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- Public Health (AREA)
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- Biodiversity & Conservation Biology (AREA)
- Clinical Laboratory Science (AREA)
- Oral & Maxillofacial Surgery (AREA)
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- Emergency Medicine (AREA)
- Obesity (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
Abstract
A split type respiration oxygen consumption real-time monitoring device comprises a split type monitoring body, wherein the split type monitoring body comprises a base and a monitoring plate arranged in a monitoring area of the split type monitoring body, and the base comprises a horizontally laid base and a light-transmitting water storage plate arranged on the surface of the base; the upper surface of the water storage plate is provided with a water storage guide groove and a water storage groove positioned at the first end part of the base; the outer side surface of the base is provided with a power supply interface, a respiration signal interface, an oxygen consumption interface and an air inlet; the monitoring plate is characterized in that a snake-shaped oxygen consumption monitoring groove is carved on the inner surface of the monitoring plate facing to a monitoring area; the light shading plate is uniformly paved with photoresistors along the length direction of the snakelike oxygen consumption monitoring groove, the photoresistors are connected with a biological signal acquisition and processing system through a resistance detection circuit, and the biological signal acquisition and processing system outputs animal oxygen consumption data according to the data of the sampling resistors. The invention has the beneficial effects that: the oxygen consumption rate in any time period in the oxygen deficiency process can be collected, and the damage of limiting oxygen consumption to animals is avoided; the experimental precision is high.
Description
Technical Field
The invention relates to a split type respiration oxygen consumption real-time monitoring device.
Background
Hypoxia is a common pathological process in various clinical diseases, and hypoxia of vital organs of brain, heart and the like is an important cause of death of organisms and is a problem which needs to be researched in plateau life, mine gallery operation and aerospace flight. In the experiments of higher medical institutions, various hypoxia experiment models are copied to simulate hypoxia, and the pathological process causing abnormal changes of metabolism, function and morphological structure of tissues is researched through the simulated hypoxia experiment models so as to explain the pathological process of various clinical diseases and explore effective treatment means of the diseases.
At present, in the oxygen deficiency experiment of medical institutions such as high-grade medical schools all over China, an oxygen deficiency measuring device: a closed system is composed of a 500ml wide-mouth bottle, a 25ml fat belly straw, a 50ml measuring cylinder and an anoxic bottle, oxygen consumption of a mouse is detected, or a 125-ml wide-mouth bottle is used for closing to directly detect anoxia resistant time, and experimental devices and methods such as a respiratory frequency is counted by a visual method at a specified time point are gradually replaced by new experimental devices and methods, such as a mouse respiratory transduction amplifier product, a mouse normal-pressure acute anoxia model device and a mouse oxygen consumption dynamic change measuring device, and a quantitative analysis system, a respiratory oxygen consumption real-time monitoring device, which can realize normal pressure and constant pressure in a closed environment and realize real-time dynamic automatic tracing of the oxygen consumption and a respiratory curve.
In the above novel oxygen-deficient device: the respiratory oxygen consumption real-time monitoring device can realize normal pressure and constant pressure in a closed environment and realize real-time dynamic automatic tracing of oxygen consumption and a respiratory curve, but has the following defects: the water storage tank and the monitoring tank are of an integrated structure, the water storage tank and the monitoring tank are connected through a channel, after the water storage tank and the monitoring tank are placed for a long time, water in the water storage tank can form water vapor atomization to the monitoring tank to form water mist or form water drops to be adhered to the upper wall of the monitoring tank, the volume of the monitoring tank is occupied, the accuracy of oxygen consumption measurement is affected (the measured value of oxygen consumption is larger than the actual value), the water mist blocks light and the irradiation intensity of the photoresistor is also affected, and the sensitivity of the measuring device is changed. The monitoring tank can be repeatedly used in a short time, trace liquid can be remained in the corner or the right angle of the monitoring tank and cannot be discharged completely, and the accurate measurement of oxygen consumption is influenced.
Disclosure of Invention
In order to overcome the defects of the real-time respiratory oxygen consumption monitoring device, the invention provides a split real-time respiratory oxygen consumption monitoring device, which is redesigned on the basis of the real-time respiratory oxygen consumption monitoring device, separates a water storage plate from a monitoring plate on the basis of keeping the functions of normal pressure, constant pressure, oxygen consumption and real-time dynamic automatic tracing of a respiratory curve to form a single integrated structure, and is convenient for replacing the monitoring plate and ensuring that a monitoring tank is dry and has no water traces and fog.
The invention relates to a split type respiration oxygen consumption real-time monitoring device, which comprises a split type monitoring body and is characterized in that: the split monitoring body comprises a base and a monitoring plate detachably mounted in a monitoring area of the split monitoring body, the base comprises a horizontally laid base and a light-transmitting water storage plate arranged on the surface of the base, the lower surface of the water storage plate is tightly attached to a light shielding plate which is laid on the surface of the base, and the part, which is not covered by the water storage plate, of the light shielding plate is used as the monitoring plate for paving the monitoring area;
the upper surface of the water storage plate is provided with a water storage guide groove and a water storage groove positioned at the first end part of the base, the water storage groove is led to the second end part of the base through the water storage guide groove, the water storage groove and the water storage guide groove are mutually communicated to form a sunken water storage cavity channel, and the bottom surface of the cavity channel of the water storage cavity channel and the upper surface of the shading plate are positioned on the same horizontal plane;
the monitoring area is provided with a baffle plate with trapezoidal orthographic projection at the position close to the water storage tank and the water storage guide groove, the inclined plane of the baffle plate is attached to the side surface of the water storage tank, the side wall of the baffle plate close to the monitoring area forms an angle of 90 degrees with the side wall of the water storage guide groove, and the angle is consistent with the edge angle of the oxygen consumption monitoring plate and is used for fixedly paving the monitoring plate;
one side of the water storage tank close to the monitoring area is provided with an inclined pipeline which can be communicated with a water inlet pipeline of the monitoring plate, the bottom of the inclined pipeline is directly inserted into the bottom of the water storage tank, the top end of the inclined pipeline is used as a water storage outlet and is flush with the upper edge of the water storage cavity, a sealing plug is arranged after the top end of the inclined pipeline extends out of the water storage tank, the sealing plug is opened, and the outlet of the inclined pipeline is connected with the water inlet of the monitoring plate through a pipeline and is used for realizing the flow of liquid between the water storage tank and;
the outer side surface of the base is provided with a power supply interface, a respiration signal interface, an oxygen consumption interface, an air inlet and an anoxic bottle interface; the power interface is provided with a power switch;
the monitoring plate is laid on the surface of a monitoring area of the base, the plate body is a colorless and transparent organic glass or acrylic plate, and the peripheral side wall of the plate body is covered with a light-tight material; a snakelike oxygen consumption monitoring groove is carved on the inner surface of the monitoring plate facing the monitoring area, and volume scales (ml) are arranged on the upper surface of the monitoring plate along the length direction of the snakelike oxygen consumption monitoring groove; the side surface of the monitoring plate facing the water storage tank is provided with a water inlet and two water outlets, the water inlet is communicated with the front end of the snakelike oxygen consumption monitoring tank, the water outlets are used for discharging water in the snakelike oxygen consumption monitoring tank after an experiment is carried out, and the water inlet and the water outlets are respectively provided with a sealing plug; the surface of the monitoring plate is provided with an air outlet which is used for being communicated with the tail end of the snakelike oxygen consumption detection groove and can be connected with a straight port of a high-sensitivity transducer which is externally arranged and used for collecting respiratory motion data, a side port of the high-sensitivity transducer is connected with an oxygen-poor bottle, and the high-sensitivity transducer is connected with a biological signal collecting and processing system; the air outlet can also be directly communicated with an air inlet, the air inlet is communicated with a built-in high-sensitivity transducer and an oxygen-deficient bottle interface, and a respiratory signal generated by the transducer is connected with a biological signal acquisition and processing system through a respiratory signal interface;
the shading plate is uniformly paved with the photoresistors along the length direction of the snakelike oxygen consumption monitoring groove, the photoresistors are kept to penetrate through the shading plate, the height of the photoresistors is flush with that of the shading plate, when the side wall of the paved monitoring plate is tightly attached to the side wall of the baffle plate and the side wall of the water storage guide groove, the photoresistors are just positioned under the snakelike oxygen consumption monitoring groove, and each photoresistor is positioned under the corresponding scale of the snakelike oxygen consumption monitoring groove; the utility model discloses a biological signal acquisition and processing system, including sampling resistor, photo resistance, sampling resistor, resistance detection circuit, biological signal acquisition and processing system, photo resistance pass through power source and external power source intercommunication, photo resistance establishes ties in proper order and forms sampling resistor, sampling resistor connect on a resistance detection circuit, resistance detection circuit's output connect biological signal acquisition and processing system, biological signal acquisition and processing system exports animal oxygen consumption data according to sampling resistor.
And a notch used for being communicated with the tail end of the water storage guide groove is reserved on the wall of the water storage groove far away from the monitoring area.
The bottom of the tank wall of the water storage tank close to the monitoring area is provided with an exhaust port communicated with the water storage guide groove (when the base is upright, the water storage tank is used for adding water and exhausting).
The water storage guiding groove is arranged along the length direction of the base, the front end of the water storage guiding groove extends to the second end part of the base, the tail end of the water storage guiding groove extends to the first end part of the base, and the tail end of the water storage guiding groove is communicated with the notch of the water storage groove, so that the water storage groove is communicated with the water storage guiding groove.
The water storage tank is a right-angle trapezoid-shaped tank, namely the orthographic projection of the water storage tank on the base is a right-angle trapezoid, and the upper bottom edge and the lower bottom edge of the right-angle trapezoid are parallel to the water storage guiding groove.
The snakelike oxygen consumption monitoring groove is a coiled snakelike groove consisting of a plurality of sections of straight grooves which are parallel to each other and curved grooves which are connected with two adjacent straight grooves, the straight grooves are arranged in rows along the length direction of the base, and the straight grooves and the curved grooves on the same side of the front end of the snakelike oxygen consumption monitoring groove are correspondingly communicated with a water outlet.
The snakelike oxygen consumption monitoring groove is 1cm in width and 0.5cm in depth.
The length of the reservoir is 1/3 the total length of the base.
The chamber top of aqua storage tank and the chamber top parallel and level of snakelike oxygen consumption monitoring groove to the vertical height in water inlet center pin place of monitoring board is less than the height at aqua storage tank chamber top border, and when not having the exogenic action, water can not follow the aqua storage tank and flow into snakelike oxygen consumption monitoring groove.
The invention has the following beneficial effects:
1. in the experiment, the liquid column for measuring oxygen consumption is in a horizontal state, so that when the liquid column moves, the liquid column does not affect the pressure in the hypoxic oxygen bottle, the problem that when water in the measuring cylinder moves to the fat belly suction pipe due to oxygen consumption of mice in a conventional oxygen consumption device through respiration, the mice lack oxygen in a negative pressure environment, and accordingly the oxygen consumption measurement result generates systematic errors is solved, and the measurement precision is effectively improved.
2. The invention solves the problem that the respiration and oxygen consumption can not be automatically monitored by a common classical oxygen depletion device, adopts the principle that pressure fluctuation caused by photoelectric induction and thoracic contraction can be transmitted in a closed container, solves the problem that the respiration frequency and the oxygen consumption are counted by a manual visual method in a conventional oxygen depletion experiment, can realize automatic tracing of the oxygen depletion oxygen consumption in any time period and tracing of the respiration curves of different types of oxygen depletion by using a respiration oxygen depletion real-time monitoring device, realizes comparison of the respiration excitability of different types of oxygen depletion experimental animals by analyzing the respiration curves, lightens the labor intensity of the experiment and improves the accuracy of the experiment result.
3. The invention redesigns and reforms the device on the basis of the real-time monitoring device of the respiration oxygen consumption, and separates the water storage plate from the monitoring plate to form an independent integrated structure on the basis of keeping the functions of normal pressure, constant pressure, oxygen consumption and real-time dynamic automatic tracing of respiration curves, and one water storage plate is provided with a plurality of monitoring plates which are matched with the water storage plate, thereby facilitating the replacement and drying of the monitoring tank. The monitoring plate can be replaced by the dry monitoring plate, so that the phenomenon that the precision of an oxygen consumption metering value is influenced due to the fact that trace liquid cannot be discharged completely in a corner or a right angle of the monitoring groove is avoided.
4. The water storage tank and the monitoring tank form an independent split structure, so that water in the water storage tank is prevented from being atomized into the monitoring tank to form water mist (water drops can be formed to be adhered to the upper wall of the monitoring tank when the water mist is too much), the volume of the monitoring tank is occupied, and the precision of oxygen consumption measurement is influenced; the water mist is prevented from blocking light, and the sensitivity of the measuring device is prevented from being influenced.
5. The change and the drying of the oxygen consumption monitoring board of being convenient for, if used repeatedly in the short time, removable clean, dry monitoring board avoids used repeatedly in integrative constructional device short time, can remain the unable clean problem of discharging of trace liquid in monitoring groove corner or the right angle, influences the precision of oxygen consumption measurement.
Drawings
FIG. 1 is a schematic structural diagram of a split type respiratory oxygen consumption real-time monitoring device;
FIG. 2 is a schematic structural diagram of a base of the split type real-time respiratory oxygen consumption monitoring device;
FIG. 3 is a schematic structural diagram of a base of the split type real-time respiratory oxygen consumption monitoring device (II);
FIG. 4 is a bottom view of a base of the split type real-time respiratory oxygen consumption monitoring device;
FIG. 5 is an isometric view of dissection of an internal cavity of a water storage plate of a base of the split type real-time monitoring device for respiration and oxygen consumption;
FIG. 6 is a top view of an oxygen consumption monitoring board of the split type respiration oxygen consumption real-time monitoring device;
FIG. 7 is an isometric view of an oxygen consumption monitoring plate of the split type real-time respiratory oxygen consumption monitoring device;
FIG. 8 is an exploded view of an oxygen consumption monitoring plate of the split type real-time respiratory oxygen consumption monitoring device;
FIG. 9 shows the measurement results of "dynamic measurement of the whole oxygen consumption of mice" in Jiang Shi, xushuxiu, etc., according to the "Chinese pharmacological report";
FIG. 10 is an automated trace of hypoxic oxygen consumption using the present invention;
wherein, 1, power interface; 2. a power switch; 3. a respiratory signal interface; 4. an oxygen consumption interface; 5. an air inlet; 6. a water filling port; 7. an air outlet; 8. a serpentine oxygen consumption monitoring cell; 9a, a first drain opening; 9b, a second water outlet; 10. a water inlet; 11. a water storage tank; 12. a baffle plate; 13. a photoresistor; 14. a visor; 15. a beveled conduit; 16. an adjustable foot; 17. capacity calibration; 18. a recess; 19. an exhaust port; 20. a water storage guiding groove; 21. an anoxic bottle interface; 22. and a monitoring plate.
Detailed Description
The invention is further described below with reference to the accompanying drawings.
With reference to the accompanying drawings:
embodiment 1 the invention relates to a split type real-time monitoring device for respiratory oxygen consumption, which comprises a split type monitoring body, wherein the split type monitoring body comprises a base and a monitoring plate arranged in a monitoring area of the split type monitoring body, the base comprises a horizontally laid base and a light-transmitting water storage plate arranged on the surface of the base, the lower surface of the water storage plate is tightly attached to a light shielding plate paved on the surface of the base, and the part, which is not covered by the water storage plate, of the light shielding plate is used as a monitoring plate 22 paved in the monitoring area;
the upper surface of the water storage plate is provided with a water storage guide groove 20 and a water storage groove 11 positioned at the first end A of the base, the water storage groove 11 is led to the second end B of the base through the water storage guide groove 20, the water storage groove 11 and the water storage guide groove 20 are mutually communicated to form a sunken water storage cavity channel, and the bottom surface of the cavity channel of the water storage cavity channel and the upper surface of the shading plate are positioned on the same horizontal plane;
a baffle plate 12 with trapezoidal orthographic projection is arranged at the position close to the water storage tank 11 and the water storage guide groove 20, the inclined plane of the baffle plate is attached to the side surface of the water storage tank 11, and the side wall of the baffle plate close to the monitoring area forms an angle of 90 degrees with the side wall of the water storage guide groove 20 and is used for fixedly paving the monitoring plate;
an inclined pipeline 15 which can be communicated with a water inlet 10 pipeline of the monitoring plate is arranged on one side of the water storage tank 11 close to the monitoring area, the bottom of the inclined pipeline 15 is directly inserted into the bottom of the water storage tank 11, the top end of the inclined pipeline 15 is used as a water storage outlet and is higher than the upper edge of the water storage cavity channel, a sealing plug is arranged after the top end of the inclined pipeline 15 extends out of the water storage tank 11, the sealing plug is opened, and the inclined pipeline outlet is connected with the water inlet 10 of the monitoring plate through a pipeline and is used for realizing the flow of liquid between the water storage tank and the;
the outer side surface of the base is provided with a power supply interface 1, a respiration signal interface 3, an oxygen consumption interface 4, an air inlet 5 and an anoxic bottle interface 21; the power interface 1 is provided with a power switch 2;
the monitoring plate 22 is laid on the surface of the monitoring area of the base, the plate body is a colorless and transparent organic glass or acrylic plate, and the peripheral side wall of the plate body is covered with a light-tight material; a snakelike oxygen consumption monitoring groove 8 is carved on the inner surface of the monitoring plate facing the monitoring area, and volume scales 17 are arranged on the upper surface of the monitoring plate 22 along the length direction of the snakelike oxygen consumption monitoring groove 8; the side surface of the monitoring plate 22 facing the water storage tank 11 is provided with a water inlet 10 and two water outlets which are respectively marked as a first water outlet 9a and a second water outlet 9b and are used for being communicated with the front end of the snakelike oxygen consumption monitoring tank 8, the first water outlet 9a and the second water outlet 9b are used for discharging water in the snakelike oxygen consumption monitoring tank 8 after an experiment, and the water inlet 10, the first water outlet 9a and the second water outlet 9b are respectively provided with a sealing plug; the surface of the monitoring plate is provided with an air outlet 7 which is used for being communicated with the tail end of the snakelike oxygen consumption detection groove 8 and can be connected with a straight port of a high-sensitivity transducer which is externally arranged and used for collecting respiratory motion data, a side port of the high-sensitivity transducer is connected with an oxygen-poor bottle, and the high-sensitivity transducer is connected with a biological signal collecting and processing system; the air outlet 7 can also be directly communicated with the air inlet 5, the air inlet 5 is communicated with a built-in high-sensitivity transducer and an oxygen-deficient bottle interface, and a respiratory signal generated by the transducer is connected with a biological signal acquisition and processing system through a respiratory signal interface 3;
the shading plate 14 is uniformly paved with the light sensitive resistors 13 along the length direction of the snakelike oxygen consumption monitoring groove, the light sensitive resistors 13 are kept to penetrate through the shading plate 14, the height of each light sensitive resistor is flush with that of the shading plate 14, when the side wall of the paved monitoring plate 22 is tightly attached to the side wall of the baffle plate 12 and the side wall of the water storage guide groove 20, the light sensitive resistors 13 are just positioned right below the snakelike oxygen consumption monitoring groove 8, and each light sensitive resistor 13 is positioned right below the corresponding scale of the snakelike oxygen consumption monitoring groove 8; the light dependent resistor pass through power source 1 and external power source intercommunication, light dependent resistor 13 establishes ties in proper order and forms the sampling resistor, the sampling resistor connect on a resistance detection circuit, resistance detection circuit's output connect biological signal acquisition processing system, biological signal acquisition processing system is according to the data output animal oxygen consumption data of sampling resistor.
The wall of the water storage groove 11 far away from the monitoring area is provided with a notch 18 which is used for communicating with the tail end of a water storage guiding groove 20.
The bottom of the tank wall of the water storage tank 11 close to the monitoring area is provided with an exhaust port 19 which is communicated with the water storage guiding groove (when the base is upright, the water storage tank is used for adding water and exhausting air).
The water storage guiding groove 20 is arranged along the length direction of the base, the front end of the water storage guiding groove 20 extends to the second end part of the base, the tail end of the water storage guiding groove 20 extends to the first end part of the base, and the tail end of the water storage guiding groove 20 is communicated with the notch 18 of the water storage groove 11, so that the water storage groove 11 is communicated with the water storage guiding groove 20.
The snakelike oxygen consumption monitoring groove 8 is a coiled snakelike groove consisting of a plurality of sections of parallel straight grooves and curved grooves connecting two adjacent straight grooves, the straight grooves are arranged in rows along the length direction of the base, and the side surface of the monitoring plate 22 is provided with at least one water outlet communicated with the bent pipe.
The snakelike oxygen consumption monitoring groove 8 is 1cm in groove width and 0.5cm in groove depth. Through long-term repeated tests, the optimal choice is found that the depth of the oxygen consumption monitoring groove 8 is 0.5cm, and the width of the groove is 1cm, ink can flow in a layered mode when the groove depth is too high, and the end face of an ink liquid column can move in a non-parallel mode when the groove is too wide, so that the accurate recording of oxygen consumption is influenced; too shallow and too narrow grooves can result in too long lumen, making the device too bulky.
The length of the reservoir 11 is 1/3 the total length of the base.
The top of the water storage tank 11 cavity is flush with the top of the snakelike oxygen consumption monitoring tank 8 cavity, the longitudinal height of the central shaft of the water inlet 10 of the monitoring plate is lower than the height of the edge of the top of the water storage tank cavity, and when no external force is applied, water cannot flow into the snakelike oxygen consumption monitoring tank 8 from the water storage tank 11.
Embodiment 2 a split type respiration oxygen consumption real-time monitoring device described in this embodiment, including split type monitoring body, split type monitoring body includes base and monitoring board, and the base comprises base and water storage plate, the water storage plate is colorless transparent organic glass or acrylic board, plate body part cover on the light screen that base upper surface laid, the region that does not cover is the monitoring area, the base is a cuboid, the bottom is equipped with adjustable foot 16 that is used for adjusting the base levelness, and aqua storage tank 11, monitoring area arrange side by side along the length direction of base; the thickness of the plate body is 1.5cm, and the depth of the water storage tank is 1 cm; the length of the water storage tank is 1/3 about the length of the base, and the length of the monitoring area is 2/3 about the length of the base;
the water storage tank 11 is a right-angled trapezoid-shaped tank, namely the orthographic projection of the water storage tank 11 on the base is a right-angled trapezoid, the upper bottom edge and the lower bottom edge of the right-angled trapezoid are both parallel to the water storage guide groove, and the right angle edge of the right-angled trapezoid is arranged along the width direction of the base and is positioned at the first end part of the base; the inclined side of the right trapezoid is arranged close to the monitoring area, a gap for accommodating the water inlet 10, the first water outlet 9a and the second water outlet 9b is reserved between the water storage tank and the monitoring plate, and the width of the gap is gradually reduced from the inner side to the outer side of the base along the width direction of the base; the bottom surface of the water storage cavity channel and the upper surface of the shading plate are positioned on the same horizontal plane; an inclined pipeline which is always inserted into the bottom of the water storage cavity is arranged on the right side of the water storage tank 11 close to the base 2/3 and serves as a water storage outlet 15, the top end of the inclined pipeline is higher than the edge of the water storage cavity, a corresponding sealing plug is matched with the inclined pipeline, and an orthographic projection trapezoid baffle plate is arranged at a position close to the water storage tank 11 and the water storage guide groove 20 and used for fixing the placing position of the oxygen consumption monitoring plate during an experiment; along the length direction of the base, the water storage groove 11 is separated from the water storage guide groove 20 through groove walls, the water storage groove and the water storage guide groove are communicated through an exhaust port 19 with the diameter of 0.6cm at the bottom of the groove walls, and the tail end of the water storage guide groove 20 is communicated with the water storage groove 11 through a notch 18. The lower surface of the water storage plate is tightly attached to the smooth light screen, the photo-resistors 13 uniformly laid in the light screen penetrate through the light screen, the height of each photo-resistor is flush with the upper surface of the light screen, so that when the edge of the oxygen consumption monitoring plate is placed at the blocking position of the baffle 12 and the water storage guide groove, the photo-resistors 13 are just right under the oxygen consumption monitoring groove 8, and each photo-resistor is right under the corresponding scale of the oxygen consumption monitoring groove 8. The photoresistor establish ties in proper order and form sampling resistor, sampling resistor connect on a resistance detection circuit, resistance detection circuit's output connect biological signal acquisition processing system, biological signal acquisition processing system exports animal oxygen consumption data according to sampling resistor's data.
The monitoring plate is a colorless transparent organic glass or acrylic plate, the peripheral side wall of the monitoring plate is covered by a lightproof material, the inner surface of the monitoring plate is carved with a snakelike oxygen consumption monitoring groove with the depth of 0.5cm and the width of 1cm, the upper surface of the monitoring plate is provided with uniform volume scales (ml) along the length direction of the oxygen consumption monitoring groove, the upper surface of the snakelike oxygen consumption monitoring groove is provided with an air outlet, the bottom of the snakelike oxygen consumption monitoring groove is communicated with a water storage outlet of the water storage tank through the oxygen consumption monitoring groove and a water inlet, and one end of the snakelike oxygen consumption monitoring groove communicated with a water storage outlet 15 of the water storage tank 11 is marked with 0 volume scale; a water inlet 10, a first water outlet 9a and a second water outlet 9b are arranged on the side surface of the monitoring plate close to the 0 volume scale in the length direction, and the water inlet 10 is connected with a water storage outlet 15 through a pipeline during an experiment; the first drain port 9a and the second drain port 9b regulate drainage by closing of the sealing plugs.
The central axis of the drain opening is located on the same horizontal plane, and the horizontal plane of the central axis of the drain opening is located above the horizontal plane of the central axis of the water inlet (see fig. 7).
The shading plate is flatly laid on the base, a power supply interface 1 is arranged on the outer side surface of the base, the photosensitive resistor 20 is communicated with an external power supply through the power supply interface 1, and the power supply interface 1 is provided with a power supply switch 2; an oxygen consumption signal interface 3 is arranged on the outer side surface of the base, and the photoresistor 13 is connected with the biological signal acquisition and processing system through the oxygen consumption signal interface 3; still be equipped with the high sensitive tension transducer who is used for communicateing anoxic bottle interface and air inlet in the base, the high sensitive tension transducer passes through respiratory signal interface 4 and links to each other with biological signal acquisition processing system, and respiratory signal interface 4 is located the lateral surface of base.
Example 3 this example differs from example 2 in that: the snakelike oxygen consumption monitoring groove is a serpentine groove which is formed by four sections of straight grooves which are parallel to each other and curved grooves which are connected with two adjacent straight grooves, the straight grooves are arranged in rows along the length direction of the base, and the two curved grooves which are on the same side with the front end of the snakelike oxygen consumption monitoring groove are respectively and correspondingly communicated with the first water outlet 9a and the second water outlet 9 b.
Example 4 the use of the split real-time respiratory oxygen consumption monitoring device of example 2 for real-time respiratory oxygen consumption monitoring comprises the following steps:
1) the water storage outlet 15 of the inclined pipeline is sealed by a sealing plug, the base is erected with the water filling port 6 upward, colored liquid diluted by distilled water is added into the capacity groove 11 from the water filling port 6 until the water level rises to a position close to the water filling port 6, and air in the water storage groove can be automatically discharged from the water filling port from the air outlet 19 to the water storage guide groove 20 in the water filling process. When the base is horizontally placed on a table top, the water storage tank is completely filled with diluted colored liquid, and the height of a liquid column is limited by the height of the tank wall of the water storage tank to be 1 cm;
2) the respiration signal interface 3 and the oxygen consumption interface 4 are connected with corresponding channels of the biological signal acquisition and processing system by signal lines, and are switched on and the power switch 2 is switched on to start preheating;
3) placing the monitoring plate on the shading plate 14, sealing the water outlet 9a and the water outlet 9b, and connecting the air inlet 5 and the air outlet 7 by pipelines;
4) the water storage outlet 15 of the inclined pipeline is connected with the pipeline, the height of the water filling port 6 is slightly raised, so that water in the water storage tank 11 flows into the pipeline connected with the water storage outlet 15 and is filled with the whole pipeline, the height of a pipeline port connected with the water filling port 6 and the water storage outlet is synchronously reduced, the whole base is restored to be in a horizontal position, the other end of the pipeline is connected with the water inlet 10, at the moment, the thickness of a bottom plate of the oxygen consumption monitoring plate is 0.5cm, the height of a cavity channel of the oxygen consumption monitoring plate is 0.5cm, the water inlet is in the middle position of the oxygen consumption monitoring tank, the height of water in the water storage tank is 1cm, the top of the cavity channel of the oxygen consumption monitoring tank and the top of the cavity channel of the water storage tank are in the same level, the position of the water inlet is 0.25cm lower than the top of the cavity channel of the water storage tank, and;
5) slightly moving the edge of the monitoring plate to the positions beside the baffle 12 and the water storage guide groove 20, so that the light-sensitive resistors 13 are just right below the monitoring groove 11, and each light-sensitive resistor 13 is right below the corresponding scale of the monitoring groove;
6) putting soda lime and a mouse into an anoxic bottle, and covering a sealing cover; then the vent pipe on the sealing cover and communicated with the oxygen-deficient bottle is communicated with the oxygen consumption monitoring groove 8 through an oxygen-deficient bottle interface;
7) starting a recording button of the biological signal acquisition and processing system, and starting an experiment; when the mouse in the anoxic bottle breathes and consumes oxygen to generate negative pressure, such as 0.5cmH2The black liquid in the water storage tank 11 flows towards the oxygen consumption monitoring tank to fill up the consumed oxygen due to the negative pressure of the O columnCapacity, the pressure in the invention is always constant at-0.5 cm H2And (4) an O column. The carbon dioxide exhaled by the mouse is absorbed by the soda lime placed in the oxygen-consuming bottle, so that the oxygen consumption of the mouse is the number of milliliters of black liquid flowing from the water storage tank 11 to the oxygen consumption monitoring tank 8 (the oxygen consumption can be read through scales on the surface of the oxygen consumption monitoring tank through manual monitoring);
8) when the black liquid in the water storage tank 11 flows to the oxygen consumption monitoring tank, the light which originally shines on the photoresistor 13 can be shielded, the more the black liquid flows into the oxygen consumption monitoring tank 8, the more the photoresistor 13 which is shielded from the light is, the more the resistance detection circuit can filter out effective signals and control voltage output by applying the signals, and the oxygen consumption is automatically traced through the biological signal acquisition and processing system;
9) when a mouse breathes, the expansion and contraction of the lung cause pressure fluctuation in the breathing oxygen consumption real-time monitoring device, the pressure fluctuation is converted into an electric signal through the high-sensitivity transducer and transmitted to the biological signal acquisition and processing system, and a breathing curve is traced by using a direct current signal, so that the pressure, the breathing frequency and the amplitude in the breathing oxygen consumption real-time monitoring device can be accurately and dynamically monitored, the breathing frequency, the amplitude and the pressure of the mouse in an anoxia experiment can be automatically monitored, and errors caused by artificial monitoring are avoided.
9) If the experiment is to be carried out again, the oxygen consumption plate is replaced, and the experiment can be carried out again according to the original operation flow.
The experimental results and advantages of the invention show that: the experimental result obtained by the oxygen consumption device is consistent with the result of a respiratory oxygen consumption real-time monitoring device (see figure 7) and also consistent with the result of dynamic measurement of the whole oxygen consumption of mice (see figure 6) in Jiang Shi, Xushuxiu and the like in China pharmacological report.
The oxygen consumption measurement result of the invention can obtain: the oxygen consumption method of different laboratories uses different volumes of the anoxic bottles, the oxygen consumption survival time is obviously different, but the oxygen consumption curves are a bidirectional curve which rises first and then falls, the oxygen consumption mainly occurs in the first half period, for example, the oxygen consumption accounts for 71% of the total oxygen consumption in the first 12 minutes and 21% of the total oxygen consumption in the second half period in the oxygen consumption measurement process of the invention, and the oxygen consumption is reduced at a constant speed; indicating that the first half of the measurement yielded a significant change in oxygen consumption (i.e., a significant difference was obtained).
The invention can collect the respiratory frequency, amplitude (ventilation) and oxygen consumption in any time period in the process of oxygen deficiency by recording the respiratory curve by the biological signal collecting and processing system, overcomes the problem that the prior device counts the respiratory frequency (the mouse breathes weakly and quickly and the respiration frequency is more than 200 times/min, and the data can not be obtained accurately on time, and even if the respiratory frequency data is obtained, the problem that the respiratory frequency and the respiratory excitation are not necessarily in positive correlation can not be corrected), and can change the conventional method of using the limited oxygen for oxygen deficiency by popularizing and using the invention, change the method of using the limited oxygen for time consumption rate or minute oxygen consumption to obtain the experimental result, and avoid the harm of the limited oxygen to animals.
On the basis of classical characteristics, the invention breaks through the recording mode of the existing anoxic device, can be said to be a systematic anoxic experimental tool integrating constant pressure, dynamic monitoring of respiratory frequency, amplitude, oxygen consumption and anoxic environmental pressure, overcomes the problem that the old device in the background technology can not recover data when reading oxygen consumption data by a visual method and missing an acquisition time point, lightens labor intensity, and has the advantages of low manufacturing cost, simple manufacture and suitability for teaching and scientific research.
Compared with the old device, the invention has the following advantages: the invention uses the biological signal acquisition and processing system to record the dynamic oxygen consumption, can acquire the oxygen consumption rate in any time period in the oxygen deficiency process, overcomes the problem that the old device can not recover the data when the old device reads the oxygen consumption data by a visual method and misses the acquisition time point, and lightens the labor intensity; the method can change the conventional method of using the limited oxygen consumption in the case of oxygen deficiency, and change the method of using the time oxygen consumption rate or the minute oxygen consumption to obtain an experimental result, thereby avoiding the damage of the limited oxygen consumption to animals; particularly, the horizontal liquid column is adopted during the experiment, so that the experiment precision is not influenced.
The embodiments described in this specification are merely illustrative of implementations of the inventive concept and the scope of the present invention should not be considered limited to the specific forms set forth in the embodiments but includes equivalent technical means as would be recognized by those skilled in the art based on the inventive concept.
Claims (9)
1. The utility model provides a split type respiration oxygen consumption real-time supervision device, includes split type monitoring body, its characterized in that: the split monitoring body comprises a base and a monitoring plate detachably mounted in a monitoring area of the split monitoring body, the base comprises a horizontally laid base and a light-transmitting water storage plate arranged on the surface of the base, the lower surface of the water storage plate is tightly attached to a light shielding plate which is laid on the surface of the base, and the part, which is not covered by the water storage plate, of the light shielding plate is used as the monitoring plate for paving the monitoring area;
the upper surface of the water storage plate is provided with a water storage guide groove and a water storage groove positioned at the first end part of the base, the water storage groove is led to the second end part of the base through the water storage guide groove, the water storage groove and the water storage guide groove are mutually communicated to form a sunken water storage cavity channel, and the bottom surface of the cavity channel of the water storage cavity channel and the upper surface of the shading plate are positioned on the same horizontal plane;
the monitoring area is provided with a baffle plate with trapezoidal orthographic projection at the position close to the water storage tank and the water storage guide groove, the inclined plane of the baffle plate is attached to the side surface of the water storage tank, the side wall of the baffle plate close to the monitoring area forms an angle of 90 degrees with the side wall of the water storage guide groove, and the angle is consistent with the edge angle of the oxygen consumption monitoring plate and is used for fixedly paving the monitoring plate;
one side of the water storage tank close to the monitoring area is provided with an inclined pipeline which can be communicated with a water inlet pipeline of the monitoring plate, the bottom of the inclined pipeline is directly inserted into the bottom of the water storage tank, the top end of the inclined pipeline is used as a water storage outlet and is flush with the upper edge of the water storage cavity, a sealing plug is arranged after the top end of the inclined pipeline extends out of the water storage tank, the sealing plug is opened, and the outlet of the inclined pipeline is connected with the water inlet of the monitoring plate through a pipeline and is used for realizing the flow of liquid between the water storage tank and;
the outer side surface of the base is provided with a power supply interface, a respiration signal interface, an oxygen consumption interface, an air inlet and an anoxic bottle interface; the power interface is provided with a power switch;
the monitoring plate is laid on the surface of a monitoring area of the base, the plate body is a colorless and transparent organic glass or acrylic plate, and the peripheral side wall of the plate body is covered with a light-tight material; a snakelike oxygen consumption monitoring groove is carved on the inner surface of the monitoring plate facing the monitoring area, and volume scales are arranged on the upper surface of the monitoring plate along the length direction of the snakelike oxygen consumption monitoring groove; the side surface of the monitoring plate facing the water storage tank is provided with a water inlet and two water outlets, the water inlet is communicated with the front end of the snakelike oxygen consumption monitoring tank, the water outlets are used for discharging water in the snakelike oxygen consumption monitoring tank after an experiment is carried out, and the water inlet and the water outlets are respectively provided with a sealing plug; the surface of the monitoring plate is provided with an air outlet which is used for being communicated with the tail end of the snakelike oxygen consumption detection groove and can be connected with a straight port of a high-sensitivity transducer which is externally arranged and used for collecting respiratory motion data, a side port of the high-sensitivity transducer is connected with an oxygen-poor bottle, and the high-sensitivity transducer is connected with a biological signal collecting and processing system; the air outlet can also be directly communicated with an air inlet, the air inlet is communicated with a built-in high-sensitivity transducer and an oxygen-deficient bottle interface, and a respiratory signal generated by the transducer is connected with a biological signal acquisition and processing system through a respiratory signal interface;
the shading plate is uniformly paved with the photoresistors along the length direction of the snakelike oxygen consumption monitoring groove, the photoresistors are kept to penetrate through the shading plate, the height of the photoresistors is flush with that of the shading plate, when the side wall of the paved monitoring plate is tightly attached to the side wall of the baffle plate and the side wall of the water storage guide groove, the photoresistors are just positioned under the snakelike oxygen consumption monitoring groove, and each photoresistor is positioned under the corresponding scale of the snakelike oxygen consumption monitoring groove; the utility model discloses a biological signal acquisition and processing system, including sampling resistor, photo resistance, sampling resistor, resistance detection circuit, biological signal acquisition and processing system, photo resistance pass through power source and external power source intercommunication, photo resistance establishes ties in proper order and forms sampling resistor, sampling resistor connect on a resistance detection circuit, resistance detection circuit's output connect biological signal acquisition and processing system, biological signal acquisition and processing system exports animal oxygen consumption data according to sampling resistor.
2. The split real-time respiratory oxygen consumption monitoring device of claim 1, wherein: and a notch used for being communicated with the tail end of the water storage guide groove is reserved on the wall of the water storage groove far away from the monitoring area.
3. The split real-time respiratory oxygen consumption monitoring device of claim 2, wherein: and the bottom of the tank wall of the water storage tank close to the monitoring area is provided with an exhaust port communicated with the water storage guide groove.
4. A split real-time respiratory oxygen consumption monitoring device as claimed in claim 2 or 3, wherein: the water storage guiding groove is arranged along the length direction of the base, the front end of the water storage guiding groove extends to the second end part of the base, the tail end of the water storage guiding groove extends to the first end part of the base, and the tail end of the water storage guiding groove is communicated with the notch of the water storage groove, so that the water storage groove is communicated with the water storage guiding groove.
5. The split real-time respiratory oxygen consumption monitoring device of claim 4, wherein: the water storage tank is a right-angle trapezoid-shaped tank, namely the orthographic projection of the water storage tank on the base is a right-angle trapezoid, and the upper bottom edge and the lower bottom edge of the right-angle trapezoid are parallel to the water storage guiding groove.
6. The split real-time respiratory oxygen consumption monitoring device of claim 1, wherein: the snakelike oxygen consumption monitoring groove is a coiled snakelike groove consisting of a plurality of sections of parallel straight grooves and curved grooves connected with two adjacent straight grooves, the straight grooves are arranged in rows along the length direction of the base, the front end of the snakelike oxygen consumption monitoring groove is communicated with the water inlet, and at least one curved groove is communicated with the water outlet.
7. The split real-time respiratory oxygen consumption monitoring device of claim 6, wherein: the snakelike oxygen consumption monitoring groove is 1cm in width and 0.5cm in depth.
8. The device for monitoring the oxygen consumption of breath with the upper drainage structure in real time as claimed in claim 3, wherein: the length of the reservoir is 1/3 the total length of the base.
9. The split real-time respiratory oxygen consumption monitoring device of claim 8, wherein: the chamber top of the aqua storage tank is flush with the chamber top of the snakelike oxygen consumption monitoring tank, the longitudinal height of the water inlet center shaft of the monitoring plate is lower than the height of the top edge of the aqua storage tank, and when no external force is applied, water cannot flow into the snakelike oxygen consumption monitoring tank from the aqua storage tank.
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| JPS619675B2 (en) * | 1976-08-12 | 1986-03-25 | Sanyo Denki Kk | |
| SU1487864A1 (en) * | 1987-06-23 | 1989-06-23 | Inst Fiziol Im A A Bogomoltsa | Chamber for determining oxygen consumption by small laboratory animals |
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