CN108742632A - A kind of toy oxygen demand real-time monitoring system with hypoxic hypoxia bottle - Google Patents
A kind of toy oxygen demand real-time monitoring system with hypoxic hypoxia bottle Download PDFInfo
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Abstract
A kind of toy oxygen demand real-time monitoring system with hypoxic hypoxia bottle, including respiration oxygen consumption real-time monitoring device and the hypoxic hypoxia bottle for providing closed lab space to toy.The present invention solves the problems, such as that common classical scarce oxygen consumption device can not monitor breathing and oxygen demand automatically, the principle that pressure oscillation can transmit in closed container caused by being shunk using optoelectronic induction and thorax, it solves the problems, such as to use artificial visual method number respiratory rate and oxygen demand in conventional anoxic experiment, the automatic of any time section hypoxic hypoxia oxygen demand may be implemented using breathing oxygen consumption real-time monitoring device to trace, the respiratory curve of different type anoxic is traced, the comparison of different type anoxic experimental animal respiratory stimulant is realized by analyzing respiratory curve, mitigate the labor intensity of experiment, improve the accuracy of experimental result.
Description
Technical field
The present invention relates to a kind of toy oxygen demand real-time monitoring systems with hypoxic hypoxia bottle.
Background technology
Anoxic is important pathogenic factor or important one of pathologic process during a variety of disease incidences of body, and high
The problem of primary work, mine tunneling and aerospace must in-flight be studied.It is commonly used in higher medical education experiment each
Kind anoxic experimental model simulates anoxic, and passes through the anoxic experimental model of simulation and carry out physiology, biochemistry, pharmacology, pathology
The research of physiology and clinic etc., to explain the pathologic process of clinical a variety of diseases, explore its effective treatment means etc..
At present in " national institution of higher learning's medical experiment programming textbook ", the classical device of non-narcotic toy anoxic experiment
A closed system is formed frequently with 500ml wide-mouth bottles, the fat tripe suction pipes of 25ml, 50ml graduated cylinders and anoxic bottle, detects the consumption of mouse
Oxygen amount (see Fig. 1, a is anoxic bottle in Fig. 1, b is soda lime, c is fat tripe suction pipe, d is graduated cylinder), or with the wide-mouth of 125-500ml
The closed direct detection hypoxia endurance time of bottle, in visual method number respiratory rate of defined time point.Mouse as experimental subjects, because
Its is small, and respiratory rate again fast (200 times/min~300 time/min), often rotates position, visual method root during anoxic
Originally the respiratory rate that can not accurately count mouse on time, causes experimental result often to be contradicted with expected results, affects experiment
Effect and quality of instruction.In addition, in conventional oxygen consumption device, mouse breathing consumption oxygen causes the water in graduated cylinder into fat tripe suction pipe
It is mobile, cause the anoxic pressure in the bottle to decline, i.e., mouse is the anoxic in subnormal ambient, makes oxygen demand measurement result generation system
Error.
Though having mouse breathing transducing amplifier product in recent years, being changed with System of organism signal and usual pressure
Energy device measures mouse breathing frequency, mouse normal pressure acute hypoxia model device and mouse oxygen demand dynamic change in closed environment
The report of measurement device, but can accomplish normal pressure, constant pressure in closed environment and realize oxygen demand and the real-time dynamic of respiratory curve certainly
The dynamic quantitative analysis device traced has not been reported.
Anoxic bottle is indispensable component part in hypoxia device, at present in " national institution of higher learning's medical experiment planning
In teaching material ", conventional anoxic bottle is lacked when the use of anoxic bottle with the wide-mouth bottle of 120 (having some schools with 250) or 500ml
Putting is：" seal is poor, when experiment carries out half, gas leak phenomenon occurs often, causes the failure of an experiment；In addition, the sodium in anoxic bottle
Or lime directly spreads bottom of bottle or with bottom of bottle is directly placed into after gauze wrapped, due to by animal after making soda lime use once
Stool and urine is polluted, it is necessary to be replaced, be caused to waste ".
Invention content
In order to overcome the disadvantages described above of hypoxia device, the present invention to provide a kind of toy oxygen consumption with hypoxic hypoxia bottle
Real-time monitoring system is measured, the present invention is on the basis of making non-constraint mouse oxygen demand by oneself, breathing dynamic syndicated monitoring hypoxia device
Hypoxia device is redesigned and is transformed, on the basis of keeping normal pressure, pressure-keeping functions, realizes that oxygen demand and respiratory curve are moved in real time
State is traced automatically.
Technical proposal that the invention solves the above-mentioned problems is：
A kind of toy oxygen demand real-time monitoring system with hypoxic hypoxia bottle, including respiration oxygen consumption monitor in real time
Device and hypoxic hypoxia bottle for providing closed lab space to toy；
The respiration oxygen consumption real-time monitoring device includes the horizontal ontology being laid with, and the ontology is the Ya Ke of water white transparency
Power plate, and the lower surface of ontology is fitted tightly on smooth barn door；Engraved in ontology capacity slot, oxygen consumption monitoring slot and into
The upper surface of gas guiding groove, ontology is equipped with air inlet and the gas outlet for being connected to hypoxic hypoxia bottle；Air inlet by into
Gas guiding groove is connected to the capacity slot；One end of the oxygen consumption monitoring slot is connected to capacity slot, and the other end is connected to the gas outlet；
The gas outlet is connected by the quick energy converter of height for acquiring respiratory movement data with anoxic bottle, and the quick energy converter of height and
System of organism signal is connected；
The junction of capacity slot and oxygen consumption monitoring slot is equipped with protrusion, and the protrusion blocks the portion of the entrance of oxygen consumption monitoring slot
Divide cross section；
The length direction for monitoring slot on the upper surface of ontology along oxygen consumption is equipped with scale；Oxygen consumption monitor slot slot bottom on along
The length direction of oxygen consumption monitoring slot is evenly provided with several photo resistance；The photo resistance is sequentially connected in series to form sampling electricity
Resistance, the sampling resistor are connected on a resistance detecting circuit, the output end connection biology of the resistance detecting circuit
Signal acquiring processing system, System of organism signal export animal oxygen demand data according to the data of sampling resistor；
The oxygen consumption monitoring slot has tortuous shape；And oxygen consumption monitoring slot includes several sections of straight troughs being mutually parallel and company
Connect the curved groove of adjacent two straight trough；
Several drainage channels and a return water guide groove are also engraved in ontology, the outlet of drainage channels is led by return water
Slot is connected to capacity slot and inlet guide slot；
Oxygen consumption monitors to be connected to by the guiding device of anti-backflow respectively between slot and the import of each drainage channels, described to lead
Stream device includes baffle and the draining pit that is located on baffle, drains between pit and baffle to have and be connected to oxygen consumption and supervise
Survey the gap of slot and drainage channels；
Draining pit opens up on the body, and baffle is respectively fixedly disposed at curved close to each of drainage channels side
Between slot and drainage channels；It is left end that baffle, which is defined, close to one end of curved groove, is right end close to one end of drainage channels；Water conservancy diversion
The top surface flush of the bottom surface of block and oxygen consumption monitoring slot, the top surface of baffle be gradually ramped up from left end to right end it is oblique
Face, and the left end on the inclined-plane extends at curved groove, the right end on the inclined-plane extends in drainage channels；
The hypoxic hypoxia bottle includes outer blank and sealing cover, and the outer screw socket of sealing cover is threadedly coupled with outer blank outside to seal
Bottleneck, and be equipped with and connect with the whorl of bottleneck of soda lime bottle soda lime bottle to be hung to the interior screw socket being located in outer blank in sealing cover；
There is the gap for accommodating toy between the bottom of bottle of soda lime bottle and the inner wall of outer blank, offered on the body of soda lime bottle several
A grid hole being connected with the inner cavity of outer blank；Snorkel is additionally provided on sealing cover, one end of snorkel is connected to soda lime bottle,
The other end is connected to the gas outlet of respiration oxygen consumption real-time monitoring device.
Further, the groove depth of capacity slot, oxygen consumption monitoring slot and inlet guide slot is 0.5cm.
Further, air inlet and gas outlet are respectively positioned on the upper left side of ontology, and capacity slot is located at the lower right of ontology.
Further, the hypoxic hypoxia bottle is with the specific connection structure of respiration oxygen consumption real-time monitoring device：Described
The side of body is equipped with the translation interface of connection gas outlet, and the other side of the ontology is external equipped with the anoxic bottle of connection snorkel
Mouthful, and translation interface is connected to anoxic bottle external tapping by high quick energy converter.
Further, the gas outlet and translation interface are detachably connected by connecting tube.
Further, straight trough right-to-left horizontal extension on the body, and straight trough and curved groove are vertically connected.
Further, the baffle is wedge type block.
Further, the bottleneck of the outer blank be equipped with matched first external screw thread of outer screw socket, the soda lime bottle
Bottleneck is equipped with and matched second external screw thread of interior screw socket.
Further, the interior screw socket is coaxially disposed with the outer screw socket, and interior screw socket is located above outer screw socket.
Further, outer screw socket is furnished with hollow circular gasket, to ensure the seal between sealing cover and outer bottleneck, simultaneously
Ensure snorkel bottom end and interior bottleneck air tight communication.
Beneficial effects of the present invention are mainly manifested in：
1, test when, the present invention measure oxygen demand fluid column be under horizontality, then fluid column move when, will not be right
Influence is caused stress in hypoxic hypoxia bottle, is solved in conventional oxygen consumption device when mouse breathing consumption oxygen leads to water in graduated cylinder
Mouse anoxic in subnormal ambient caused by when being moved into fat tripe suction pipe, to make oxygen demand measurement result generation system miss
The problem of difference, the present invention effectively increase measurement accuracy.
2, respiration oxygen consumption real-time monitoring device is erect, and oxygen consumption monitoring slot is upward, capacity slot downward, flows into experiment
Water in oxygen consumption monitoring slot can flow to capacity slot by drainage channels, and draining is very convenient, while also avoiding testing every time
Need the trouble for adding water.
3, due to the presence of soda lime bottle, ensureing toy energy small range activity while limiting anoxic animal in anoxic bottle
In activity space, avoid the aggravating activities of animal from influencing respiratory movement curve and trace；The presence of built-in ceiling mounting type soda lime,
The carbon dioxide for absorbing the exhalation of anoxic animal, avoids the generation of hypercapnia；In addition, in eminence soda lime be not easy by
The pollution of mouse stool and urine extends the usage time of soda lime, and it is convenient to replace, and mitigates the labor intensity of experiment and improves anoxic reality
The success rate tested.
4, the present invention solves the problems, such as that common classical scarce oxygen consumption device can not monitor breathing and oxygen demand automatically, using light
The principle that pressure oscillation can transmit in closed container caused by electric induction and thorax are shunk solves to use in conventional anoxic experiment
Any time section may be implemented using breathing oxygen consumption real-time monitoring device in the problem of artificial visual method number respiratory rate and oxygen demand
The automatic of hypoxic hypoxia oxygen demand is traced, and the respiratory curve of different type anoxic is traced, and is realized not by analyzing respiratory curve
The comparison of same type anoxic experimental animal respiratory stimulant, mitigates the labor intensity of experiment, improves the accuracy of experimental result.
Description of the drawings
Fig. 1 is the structural schematic diagram of the classical device of non-narcotic toy anoxic experiment；
Fig. 2 is the vertical view one for breathing oxygen consumption real-time monitoring device；
Fig. 3 is the internal passage anatomical axis survey one for breathing oxygen consumption real-time monitoring device；
Fig. 4 is the axonometric drawing one for breathing oxygen consumption real-time monitoring device；
Fig. 5 is the axonometric drawing two for breathing oxygen consumption real-time monitoring device；
Fig. 6 is basis《Chinese Pharmacological Bulletin》" dynamic of mouse Whole Oxygen Consumption measures dynamic and surveys by Jiang Zhiwen, Xu Shuxiu etc.
Measurement result calmly "；
Fig. 7 is to carry out the automatic of hypoxic hypoxia oxygen demand using the present invention to trace result；
Fig. 8 is the explosive view of hypoxic hypoxia bottle of the present invention；
Fig. 9 is the structural schematic diagram of outer blank of the present invention；
Figure 10 is the upward view of sealing cover of the present invention；
Figure 11 is the vertical view of sealing cover of the present invention.
Figure 12 is the axonometric drawing of soda lime bottle of the present invention.
In figure：01. power interface, 02. power switch, 03. oxygen consumption signaling interface, 04. breath signal interface, 05. capacity
Slot, 06. drainage channels 1, the protrusion of 07. drainage channels 2,08., 09. repair slot, 10~13. repair slots, 14. inlet guide slots,
15. oxygen consumption monitors slot, 16. anoxic bottle external tappings, 17. translation interfaces, 18. gas outlets, 19. air inlets, 20. photo resistance, 21
~22 baffles, 23~24 draining pits, 25 sealing covers, 26. soda lime bottles, 27. outer blanks, 28. outer blank screw socket, 29. soda lime bottle
30. sealing cover internal thread of blow vent, 31. sealing cover external screw threads, 32. gaskets, 33. snorkels.
Specific implementation mode
With reference to attached drawing, a kind of toy oxygen demand real-time monitoring system with hypoxic hypoxia bottle, including breathing oxygen consumption
Measure real-time monitoring device and the hypoxic hypoxia bottle for providing closed lab space to toy；
The respiration oxygen consumption real-time monitoring device includes the horizontal ontology being laid with, and the ontology is the Ya Ke of water white transparency
Power plate, and the lower surface of ontology is fitted tightly on smooth barn door；Capacity slot 05, oxygen consumption monitoring slot 15 have been engraved in ontology
It is equipped with air inlet 19 and the gas outlet 18 for being connected to anoxic bottle with the upper surface of inlet guide slot 14, ontology；Air inlet 19
It is connected to the capacity slot 05 by inlet guide slot 14；One end connection capacity slot 05 of the oxygen consumption monitoring slot 15, the other end
It is connected to the gas outlet 18；The gas outlet 18 passes through the quick energy converter of height and anoxic bottle phase for acquiring respiratory movement data
Even, and the quick energy converter of height is connected with System of organism signal；
Capacity slot 05 and the junction of oxygen consumption monitoring slot 15 are equipped with protrusion, and the protrusion blocks entering for oxygen consumption monitoring slot 15
The partial cross sectional of mouth；
The length direction for monitoring slot 15 on the upper surface of ontology along oxygen consumption is equipped with scale；Oxygen consumption monitors on the slot bottom of slot 15
The length direction that slot 15 is monitored along oxygen consumption is evenly provided with several photo resistance 20；The photo resistance 20 is sequentially connected in series
Sampling resistor is formed, the sampling resistor is connected on a resistance detecting circuit, the output of the resistance detecting circuit
End connection System of organism signal, System of organism signal export animal oxygen consumption according to the data of sampling resistor
Measure data；
The oxygen consumption monitoring slot 15 has tortuous shape；And oxygen consumption monitoring slot 15 includes several sections of straight troughs being mutually parallel
With the curved groove for connecting adjacent two straight trough；
Several drainage channels 06,07 and a return water guide groove are also engraved in ontology, the outlet of drainage channels 06,07 is equal
It is connected to capacity slot 05 and inlet guide slot 14 by return water guide groove；
Oxygen consumption monitors to be connected by the guiding device of anti-backflow respectively between slot 15 and the import of each drainage channels 06,07
Logical, the guiding device includes baffle and the draining pit 23,24 that is located on baffle, drains pit 23,24 and water conservancy diversion
There is the gap of connection oxygen consumption monitoring slot 15 and drainage channels 06,07 between block 21,22；
Draining pit 23,24 opens up on the body, and baffle 21,22 is respectively fixedly disposed at close to drainage channels 19
Between each curved groove and drainage channels 06,07 of side；It is left end that baffle, which is defined, close to one end of curved groove, close to drainage channels
06,07 one end is right end；The top surface flush of the bottom surface of baffle 21,22 and oxygen consumption monitoring slot 15, the top of baffle 21,22
Face is the inclined-plane gradually ramped up from left end to right end, and the left end on the inclined-plane extends at curved groove, the right side on the inclined-plane
End extends in drainage channels 06,07.
Baffle 21,22 can flow to oxygen consumption monitoring slot 15 by barrier liquid when ontology is horizontally arranged from drainage channels 06,07,
Liquid can be guided to be flowed in drainage channels 06,07 from oxygen consumption monitoring slot 15 when ontology is placed vertically again.
The hypoxic hypoxia bottle includes outer blank 27 and sealing cover 25, and outer screw socket 31 and the outer blank screw thread of sealing cover 25 connect
It connects, to seal 27 mouthfuls of outer blank, and is equipped with and connect with the whorl of bottleneck of soda lime bottle 26 to hang soda lime bottle 26 in sealing cover 25
The interior screw socket 30 being located in outer blank；There is the gap for accommodating toy, sodium stone between the bottom of bottle of soda lime bottle and the inner wall of outer blank
Several grid holes being connected with the inner cavity of outer blank 27 are offered on the body of grey bottle 26；It is additionally provided with snorkel on sealing cover 25
33, one end of snorkel 33 is connected to soda lime bottle 26, and the gas outlet 18 of the other end and respiration oxygen consumption real-time monitoring device connects
It is logical.
Oxygen consumption monitoring slot 15 is good quick to be shown to the breathing of animal in experiment suitable for making the elongated slot of small cross section
Sensitivity, even if the breathing of mouse can also cause the fluid column of ink to generate apparent movement.
The groove depth of capacity slot 05, oxygen consumption monitoring slot 15 and inlet guide slot 14 is 0.5cm.By prolonged and repeated experiment,
It was found that it is that 1cm is optimal selection that the groove depth of oxygen consumption monitoring slot 15, which takes 0.5cm, width, groove depth is excessively high, and laminar flow can occur for ink,
Slot is wide, and the end face of ink fluid column occurs not move concordantly, influences the accurate recording of oxygen demand；Groove depth is excessively shallow, narrow, can lead
It causes tube chamber long, keeps device excessively huge.
Air inlet 19 and gas outlet 18 are respectively positioned on the upper left side of ontology, and capacity slot 05 is located at the lower right of ontology.
The hypoxic hypoxia bottle is with the specific connection structure of respiration oxygen consumption real-time monitoring device：The side of the ontology
Translation interface 17 equipped with connection gas outlet 18, the other side of the ontology are equipped with the anoxic bottle external tapping 16 of connection snorkel,
And translation interface 17 is connected to anoxic bottle external tapping 16 by high quick energy converter.The gas outlet 18 and translation interface 17 pass through company
It takes over and is detachably connected, and the connecting leg is silicone tube.
The gas outlet 18 and translation interface 17 are detachably connected by connecting tube.
Straight trough horizontal extension from left to right on the body, and straight trough and curved groove are vertically connected.
The baffle 21,22 is wedge type block.
The bottleneck of the outer blank 27 is equipped with and 31 matched first external screw thread of outer screw socket, the bottle of the soda lime bottle 26
Mouth is equipped with and 30 matched second external screw thread of interior screw socket.
The interior screw socket 30 is coaxially disposed with the outer screw socket 31, and interior screw socket 30 is located at 31 top of outer screw socket.
Outer screw socket 31 is furnished with hollow circular gasket, to ensure the seal between 27 mouthfuls of sealing cover 25 and outer blank, simultaneously
Ensure 33 bottom end of snorkel and interior bottleneck air tight communication.
1, respiration oxygen consumption real-time monitoring device
Barn door is laid on pedestal, and the lateral surface of pedestal is equipped with power interface 01, and photo resistance 20 is connect by power supply
Mouth 01 is connected to extraneous power supply, and power interface 01 is furnished with power switch 02；The lateral surface of pedestal is equipped with oxygen consumption signaling interface
03, photo resistance 20 is connected by oxygen consumption signaling interface 03 with System of organism signal；
It is 0.5cm that the width that oxygen consumption monitors slot 15, which is 1cm groove depths, can accommodate the capacity of 45-55ml water in total.
About 5mm*5mm is engraved or is cast between inlet guide slot 14, capacity slot 05, oxygen consumption monitoring slot 15, drainage channels 06,07
Deep and wide rectangle repairs slot 09,10,11,12,13.
Capacity slot 05, oxygen consumption monitoring slot 15, inlet guide slot 14, the drainage channels 06,07 engraved in ontology can both be engraved
It is engraved in body interior, can also be engraved on the lower surface of ontology.But it engraves in body interior, capacity slot 05, oxygen consumption monitoring
Slot 15, inlet guide slot 14, drainage channels 19 and return water guide groove slot bottom need to be located on the same horizontal plane；And it engraves in ontology
When on lower surface, then the slot bottom of each slot is served as by barn door.
When respiration oxygen consumption real-time monitoring device is horizontal positioned, when without outer force effect, 0.5cm thickness in capacity slot 05
Ink will not be flowed under the action of surface tension and adhesiveness, so experiment start before, the water in capacity slot 05 is not
It can be flowed into oxygen consumption monitoring slot 15.Meanwhile protrusion has blocked the partial cross sectional of the entrance of oxygen consumption monitoring slot 15 so that oxygen consumption
The flow resistance for monitoring the ink of slot 15 inlet increases, and further ensures ink before experiment starts in capacity slot 05 not
It can be flowed into oxygen consumption monitoring slot 15.
After experiment starts, ink overcomes the resistance of 15 inlet of oxygen consumption monitoring slot to flow under the traction of the negative pressure of anoxic bottle
Oxygen consumption monitors in slot 15, and flows to gas outlet 18 along oxygen consumption monitoring slot 15.The ink in capacity slot 05 is reduced at this time, air inlet
19 by inlet guide slot 14 to 5 make-up gas of capacity slot.
When experiment (ontology is horizontal positioned), guiding device will be encountered when ink flows through when oxygen consumption monitors the curved groove of slot 15, will be led
Stream block 21,22 can prevent the liquid medium in capacity slot 05 from pouring in down a chimney in oxygen consumption monitoring slot 15.Drain the base opening of pit 23,24
The either flush of place plane and oxygen consumption monitoring slot 15, under the effect of gravity, the liquid medium in oxygen consumption monitoring slot 15 will not be filled out
The gap between draining pit 23,24 and baffle 21,22 is filled, therefore, guiding device not will produce oxygen demand measurement result
It influences.
The length of ink fluid column reflects the oxygen demand of experimental animal, and ink fluid column floods photo resistance 20 and causes sampling electricity
The change in resistance of resistance, oxygen consumption monitor photo resistance 20 regularly arranged under slot 15 and can be used for automatic sensing oxygen demand.Its principle is
When stronger using photo resistance light, resistance value is relatively low, then resistance larger feature when dark, using natural light or light as
Transmitting terminal, as receiving terminal, oxygen consumption monitors 20 resistance value of photo resistance flooded by ink fluid column in slot 15 and occurs for photo resistance
Variation.Light signal is converted into electric signal using the resistance detecting circuit of electric bridge form, passes through biochips diagnosis system
System software shows the trend curve of oxygen demand, by detection signal digitlization.
2, hypoxic hypoxia bottle
Outer blank 23 can be used common 120ml anoxic bottles and serve as.
Hypoxic hypoxia bottle is the corollary apparatus for breathing oxygen consumption real-time monitoring device, is the component part of the present invention,
Higher medical universities and colleges generally use 120ml wide-mouth bottles as the anoxic bottle of common common hypoxic hypoxia at present, no
It can guarantee the seal of oxygen depletion system in experimentation, soda lime is put in anoxic bottom of bottle portion with gauze wrapped, easily by mouse size
Just pollution, prevents soda lime from reusing.
Hypoxic hypoxia bottle is connected through a screw thread with sealing cover, and is further sealed using gasket (silicagel pad), with true
The leakproofness for protecting anoxic experimental system can significantly improve the success rate of anoxic experiment.Built-in ceiling mounting type screw socket soda lime bottle 26, makes
Soda lime is not easy to be polluted by mouse stool and urine, extends the usage time of soda lime, and addition soda lime is more accurate, convenient fast
Victory, can mitigate the labor intensity of experiment, and the activity space of the also restricted anoxic experimental animal of ceiling mounting type screw socket soda lime bottle is conducive to
The automation of respiratory movement curve records.
Due to the presence of soda lime bottle 26, is ensureing animal energy small range activity while limiting anoxic animal in anoxic bottle
Activity space, avoid the aggravating activities of animal from influencing respiratory movement curve and trace；The presence of built-in ceiling mounting type soda lime is inhaled
The carbon dioxide for having received the exhalation of anoxic animal, avoids the generation of hypercapnia；In addition, the soda lime in eminence is not easy by small
The pollution of mouse stool and urine extends the usage time of soda lime, and it is convenient to replace, and mitigates the labor intensity of experiment and improves anoxic experiment
Success rate.The anoxic bottle external tapping 17 for being measured slot with breathing oxygen consumption induction using this anoxic bottle is connected, you can measures oxygen demand
With respiratory movement curve；In addition, since the mouse that anaerobic environment internal pressure is always held in anoxic bottle in oxygen consumption experimentation exhales
It inhales under the negative pressure that consumption oxygen generates, such as -0.5cmH2O, be equivalent to oxygen consumption experiment it is identical as the condition that weary oxygen is tested, therefore,
The observation index that the behavior of animal and color change during anoxic can be used as hypoxic hypoxia is observed simultaneously, and it is real to change tradition
Oxygen demand and hypoxic hypoxia need independent experimental method in proved recipe method, can save animal or reduce the injury to animal.
3, use step of the invention is：
1) respiration oxygen consumption real-time monitoring device is erected, and capacity slot 05 is downward, oxygen consumption monitors slot 15 upward；Use silica gel
Pipe is connected to gas outlet 18 and translation interface 17；Again from the black that addition was diluted with distilled water into capacity slot 05 in air inlet 19
Ink to water level rises at 0 scale of oxygen consumption monitoring slot 15 (i.e. the inlet of oxygen consumption monitoring slot 15).
2) respiration oxygen consumption real-time monitoring device is horizontal positioned on the table, and breath signal interface 04 is adopted with bio signal
The respective channel for collecting processing system is connected；Then by the respective channel of oxygen consumption signaling interface 03 and System of organism signal
It is connected；Finally power interface 01 is connect with power supply.
3) before testing, 25g soda limes are packed into the soda lime bottle 26 of 50ml；Toy to be tested is packed into outer blank 27
It is interior, the sealing cover 25 of hypoxic hypoxia bottle is then covered, the snorkel 33 on sealing cover 25 is finally passed through into anoxic bottle external tapping
16 connections.
4) startup power supply switch 02, experiment starts.When mouse breathing consumption oxygen in anoxic bottle generates negative pressure, such as
0.5cmH2O columns, the ink in capacity slot 05 can fill up consumption due to the effect of negative pressure to the 15 direction flowing of oxygen consumption monitoring slot
Oxygen capacity makes the pressure in respiration oxygen consumption real-time monitoring device be constant all the time in -0.5cm H2O columns.Mouse breathes out titanium dioxide
Carbon is absorbed by the soda lime placed in consumption anoxic bottle again, and therefore, capacity slot 05 flows to the milliliter of ink in oxygen consumption monitoring slot 15
Number is the oxygen demand of mouse (scale that personal monitoring can monitor 15 surface of slot by oxygen consumption reads oxygen demand)；
When the dilution ink in capacity slot 05 is flowed to oxygen consumption monitoring slot 15, you can flood photo resistance 20, shelter from
Original light impinged upon in photo resistance 20, the ink for flowing into oxygen consumption monitoring slot 15 is more, and the photo resistance 20 being blocked is more
More, resistance detecting circuit can filter out useful signal and application signal control voltage output, by biochips diagnosis
System traces out oxygen demand automatically.
When mouse breathing, the expansion and contraction of lung cause to breathe the pressure oscillation in oxygen consumption real-time monitoring device, pass through height
Quick energy converter is transformed into electric signal and is transmitted to RM6240 System of organism signal, and respiratory curve is traced out with direct current signal,
Accurately dynamic monitoring the pressure in oxygen consumption real-time monitoring device, respiratory rate and amplitude can be breathed, realize anoxic experiment mice
The automatic monitoring of respiratory rate, amplitude and pressure, avoids error caused by personal monitoring.
5) to be tested again, respiration oxygen consumption real-time monitoring device is erect, and oxygen consumption monitoring slot 15 is upward, holds
Downward, oxygen consumption monitors the water in slot 15 can be by clearance flow between draining between pit 23,24 and drainage channels 06,07 for measuring tank 05
To capacity slot 05.It drains after flowing into the water in oxygen consumption monitoring slot 15 in experiment, then breathing oxygen consumption real-time monitoring device level is put
It sets on the table, you can tested again, avoid experiment every time and need the trouble for adding water.
2, experimental result of the invention and advantage explanation
According to《Chinese Pharmacological Bulletin》Jiang Zhiwen, Xu Shuxiu's etc. " dynamic of mouse Whole Oxygen Consumption measures dynamic and measures "
As a result the measurement result (see Fig. 7) of oxygen demand is carried out (see Fig. 6) and using the present invention to be obtained：The oxygen consumption experiment of different experiments room
The capacity of used anoxic bottle is different, and there is a notable difference in the oxygen consumption time-to-live, but oxygen demand curve be in one first rise after under
The two-way curve of drop, oxygen consumption occurs mainly in the front half section time, such as is carried out in oxygen demand measurement first 12 minutes using the present invention
The 71% of total oxygen demand is accounted for, later half time oxygen demand accounts for the 21% of total amount, and is in uniform descent；When illustrating to measure front half section
Between can be obtained the oxygen demand result of difference (obtain) for changing meaning.
Due to present invention System of organism signal recording respiration curve, when can acquire any during anoxic
Between section respiratory rate, amplitude (ventilatory capacity) and oxygen demand, overcome old device visual method number respiratory rate (mouse breathing it is weak and
Soon,>200 times/min, the problem of can not accurately obtaining data on time, even if obtaining the data of respiratory rate, it can not correct and exhale
Inhale the problem of frequency is not necessarily positively correlated with respiratory stimulant) and oxygen demand, it can change routine by promoting the use of the present invention
The way of anoxic operating limit oxygen consumption, the way for making time oxygen consumption rate or minute oxygen demand into obtain experimental result, avoid pole
Limit oxygen consumption murders animal.
The present invention breaks through the recording mode of existing hypoxia device on the basis of succession classical characteristic, it may be said that is that collection is permanent
Pressure, dynamic monitoring respiratory rate, amplitude, oxygen demand and anaerobic environment pressure are the systematization anoxic experimental tool of the whole body, are overcome
The problem of old device in background technology reads oxygen demand data with visual method, and data can not be restored by missing acquisition time just,
And alleviate labor intensity, and it is cheap, make it is simple, be suitable for teaching and scientific research.
Compared with old device, the present invention has following advantage：
The present invention records dynamic oxygen demand with System of organism signal, can acquire any time during anoxic
The oxygen consumption rate of section overcomes old device visual method to read oxygen demand data, misses acquisition time and can not just restore data
Problem, and alleviate labor intensity；The way that conventional anoxic operating limit oxygen consumption can be changed using the present invention makes time consumption into
The way of oxygen rate or minute oxygen demand obtains experimental result, and Limiting Oxygen is avoided to murder animal；It is of particular importance that this
Horizontal fluid column is used when invention experiment, does not interfere with experimental precision.
Content described in this specification embodiment is only enumerating to the way of realization of inventive concept, protection of the invention
Range is not construed as being only limitted to the concrete form that embodiment is stated, protection scope of the present invention also includes art technology
Personnel according to present inventive concept it is conceivable that equivalent technologies mean.
Claims (10)
1. a kind of toy oxygen demand real-time monitoring system with hypoxic hypoxia bottle, it is characterised in that：Including breathing oxygen consumption
Measure real-time monitoring device and the hypoxic hypoxia bottle for providing closed lab space to toy；
The respiration oxygen consumption real-time monitoring device includes the horizontal ontology being laid with, and the ontology is the acrylic of water white transparency
Plate, and the lower surface of ontology is fitted tightly on smooth barn door；Capacity slot, oxygen consumption monitoring slot and air inlet have been engraved in ontology
The upper surface of guiding groove, ontology is equipped with air inlet and the gas outlet for being connected to hypoxic hypoxia bottle；Air inlet passes through air inlet
Guiding groove is connected to the capacity slot；One end of the oxygen consumption monitoring slot is connected to capacity slot, and the other end is connected to the gas outlet；Institute
It states gas outlet by the quick energy converter of height for acquiring respiratory movement data to be connected with anoxic bottle, and the quick energy converter of the height and life
Object signal acquiring processing system is connected；
The junction of capacity slot and oxygen consumption monitoring slot is equipped with protrusion, and the protrusion blocks the part cross of the entrance of oxygen consumption monitoring slot
Section；
The length direction for monitoring slot on the upper surface of ontology along oxygen consumption is equipped with scale；Oxygen consumption monitors on the slot bottom of slot along oxygen consumption
The length direction of monitoring slot is evenly provided with several photo resistance；The photo resistance is sequentially connected in series to form sampling resistor,
The sampling resistor is connected on a resistance detecting circuit, and the output end of the resistance detecting circuit connects bio signal
Acquisition processing system, System of organism signal export animal oxygen demand data according to the data of sampling resistor；
The oxygen consumption monitoring slot has tortuous shape；And oxygen consumption monitoring slot includes several sections of straight troughs being mutually parallel and connection phase
The curved groove of adjacent two straight troughs；
Several drainage channels and a return water guide groove are also engraved in ontology, the outlet of drainage channels is connected by return water guide groove
Logical capacity slot and inlet guide slot；
Oxygen consumption monitors to be connected to by the guiding device of anti-backflow respectively between slot and the import of each drainage channels, the water conservancy diversion dress
It sets including baffle and the draining pit being located on baffle, has between draining pit and baffle and be connected to oxygen consumption monitoring slot
With the gap of drainage channels；
Draining pit opens up on the body, baffle be respectively fixedly disposed at each curved groove close to drainage channels side with
Between drainage channels；It is left end that baffle, which is defined, close to one end of curved groove, is right end close to one end of drainage channels；Baffle
The top surface flush of bottom surface and oxygen consumption monitoring slot, the top surface of baffle is the inclined-plane gradually ramped up from left end to right end, and
The left end on the inclined-plane extends at curved groove, and the right end on the inclined-plane extends in drainage channels；
The hypoxic hypoxia bottle includes outer blank and sealing cover, and the outer screw socket of sealing cover is threadedly coupled with outer blank to seal outer blank
Mouthful, and be equipped with and connect with the whorl of bottleneck of soda lime bottle soda lime bottle to be hung to the interior screw socket being located in outer blank in sealing cover；Sodium
There is the gap for accommodating toy between the bottom of bottle of lime bottle and the inner wall of outer blank, several are offered on the body of soda lime bottle
The grid hole being connected with the inner cavity of outer blank；Snorkel is additionally provided on sealing cover, one end of snorkel is connected to soda lime bottle, separately
One end is connected to the gas outlet of respiration oxygen consumption real-time monitoring device.
2. a kind of toy oxygen demand real-time monitoring system with hypoxic hypoxia bottle as described in claim 1, feature
It is：The groove depth of capacity slot, oxygen consumption monitoring slot and inlet guide slot is 0.5cm.
3. a kind of toy oxygen demand real-time monitoring system with hypoxic hypoxia bottle as claimed in claim 2, feature
It is：Air inlet and gas outlet are respectively positioned on the upper left side of ontology, and capacity slot is located at the lower right of ontology.
4. a kind of toy oxygen demand real-time monitoring system with hypoxic hypoxia bottle as claimed in claim 3, feature
It is：The hypoxic hypoxia bottle is with the specific connection structure of respiration oxygen consumption real-time monitoring device：The side of the ontology is set
There is the translation interface of connection gas outlet, the other side of the ontology is equipped with the anoxic bottle external tapping of connection snorkel, and conversion connects
Mouth is connected to anoxic bottle external tapping by high quick energy converter.
5. a kind of toy oxygen demand real-time monitoring system with hypoxic hypoxia bottle as claimed in claim 4, feature
It is：The gas outlet and translation interface are detachably connected by connecting tube.
6. a kind of toy oxygen demand real-time monitoring system with hypoxic hypoxia bottle as claimed in claim 5, feature
It is：Straight trough right-to-left horizontal extension on the body, and straight trough and curved groove are vertically connected.
7. a kind of toy oxygen demand real-time monitoring system with hypoxic hypoxia bottle as claimed in claim 6, feature
It is：The baffle is wedge type block.
8. a kind of toy oxygen demand real-time monitoring system with hypoxic hypoxia bottle as claimed in claim 7, feature
It is：The bottleneck of the outer blank is equipped with to be equipped with matched first external screw thread of outer screw socket, the bottleneck of the soda lime bottle
With matched second external screw thread of interior screw socket.
9. a kind of toy oxygen demand real-time monitoring system with hypoxic hypoxia bottle as claimed in claim 8, feature
It is：The interior screw socket is coaxially disposed with the outer screw socket, and interior screw socket is located above outer screw socket.
10. a kind of toy oxygen demand real-time monitoring system with hypoxic hypoxia bottle as claimed in claim 9, feature
It is：Outer screw socket is furnished with hollow circular gasket, to ensure the seal between sealing cover and outer bottleneck, while ensureing snorkel
Bottom end and interior bottleneck air tight communication.
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| CN201810149700.5A CN108742632B (en) | 2018-02-13 | 2018-02-13 | Real-time oxygen consumption monitoring system for small animals with hypoxic anoxic bottles |
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| CN201810149700.5A CN108742632B (en) | 2018-02-13 | 2018-02-13 | Real-time oxygen consumption monitoring system for small animals with hypoxic anoxic bottles |
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| CN108742632A true CN108742632A (en) | 2018-11-06 |
| CN108742632B CN108742632B (en) | 2020-04-28 |
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| CN110136835A (en) * | 2019-05-29 | 2019-08-16 | 西南交通大学 | The determination method, apparatus and electronic equipment of physical labor intensity |
| CN110623670A (en) * | 2019-08-16 | 2019-12-31 | 浙江大学 | Split type respiration oxygen consumption real-time monitoring device |
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| Publication number | Publication date |
|---|---|
| CN108742632B (en) | 2020-04-28 |
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