CN108272455A - A kind of toy oxygen demand real-time monitoring system with modularization water seal anoxic bottle - Google Patents
A kind of toy oxygen demand real-time monitoring system with modularization water seal anoxic bottle Download PDFInfo
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- A61B5/08—Detecting, measuring or recording devices for evaluating the respiratory organs
- A61B5/083—Measuring rate of metabolism by using breath test, e.g. measuring rate of oxygen consumption
- A61B5/0833—Measuring rate of oxygen consumption
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Abstract
A kind of toy oxygen demand real-time monitoring system with modularization water seal anoxic bottle, including respiration oxygen consumption real-time monitoring device and the modularization water seal anoxic bottle for providing closed lab space to toy.The present invention solves the problems, such as that common classical scarce oxygen consumption device can not monitor breathing and oxygen demand automatically, the principle that pressure oscillation can transmit in closed container caused by being shunk using optoelectronic induction and thorax, it solves the problems, such as to use artificial visual method number respiratory rate and oxygen demand in conventional anoxic experiment, the automatic of any time section hypoxic hypoxia oxygen demand may be implemented using breathing oxygen consumption real-time monitoring device to trace, the respiratory curve of different type anoxic is traced, the comparison of different type anoxic experimental animal respiratory stimulant is realized by analyzing respiratory curve, mitigate the labor intensity of experiment, improve the accuracy of experimental result.Modularization water seal anoxic bottle has done double-encryption on the leakage problem for easily cause the failure of an experiment：It can ensure that experiment will not gas leakage and failure.
Description
Technical field
The present invention relates to a kind of toy oxygen demand real-time monitoring systems with modularization water seal anoxic bottle.
Background technology
Anoxic is important pathogenic factor or important one of pathologic process during a variety of disease incidences of body, and high
The problem of primary work, mine tunneling and aerospace must in-flight be studied.It is commonly used in higher medical education experiment each
Kind anoxic experimental model simulates anoxic, and passes through the anoxic experimental model of simulation and carry out physiology, biochemistry, pharmacology, pathology
The research of physiology and clinic etc., to explain the pathologic process of clinical a variety of diseases, explore its effective treatment means etc..
At present in " national institution of higher learning's medical experiment programming textbook ", the classical device of non-narcotic toy anoxic experiment
A closed system is formed frequently with 500ml wide-mouth bottles, the fat tripe suction pipes of 25ml, 50ml graduated cylinders and anoxic bottle, detects the consumption of mouse
Oxygen amount (see Fig. 1, a is anoxic bottle in Fig. 1, b is soda lime, c is fat tripe suction pipe, d is graduated cylinder), or with the wide-mouth of 125-500ml
The closed direct detection hypoxia endurance time of bottle, in visual method number respiratory rate of defined time point.Mouse as experimental subjects, because
Its is small, and respiratory rate again fast (200 times/min~300 time/min), often rotates position, visual method root during anoxic
Originally the respiratory rate that can not accurately count mouse on time, causes experimental result often to be contradicted with expected results, affects experiment
Effect and quality of instruction.In addition, in conventional oxygen consumption device, mouse breathing consumption oxygen causes the water in graduated cylinder into fat tripe suction pipe
It is mobile, cause the anoxic pressure in the bottle to decline, i.e., mouse is the anoxic in subnormal ambient, makes oxygen demand measurement result generation system
Error.
Though having mouse breathing transducing amplifier product in recent years, being changed with System of organism signal and usual pressure
Energy device measures mouse breathing frequency, mouse normal pressure acute hypoxia model device and mouse oxygen demand dynamic change in closed environment
The report of measurement device, but can accomplish normal pressure, constant pressure in closed environment and realize oxygen demand and the real-time dynamic of respiratory curve certainly
The dynamic quantitative analysis device traced has not been reported.
Anoxic bottle is indispensable component part in hypoxia device, at present in " national institution of higher learning's medical experiment planning
In teaching material ", conventional anoxic bottle is lacked when the use of anoxic bottle with the wide-mouth bottle of 120 (having some schools with 250) or 500ml
Putting is：" seal is poor, when experiment carries out half, gas leak phenomenon occurs often, causes the failure of an experiment；In addition, the sodium in anoxic bottle
Or lime directly spreads bottom of bottle or with bottom of bottle is directly placed into after gauze wrapped, due to by animal after making soda lime use once
Stool and urine is polluted, it is necessary to be replaced, be caused to waste ".And anoxic bottle has a single function, single bottle is applied alone, and practicability is not strong.
Invention content
In order to overcome the disadvantages described above of hypoxia device, the present invention to provide a kind of toy with modularization water seal anoxic bottle
Oxygen demand real-time monitoring system, the present invention is in the base for making non-constraint mouse oxygen demand, the dynamic syndicated monitoring hypoxia device of breathing by oneself
Hypoxia device is redesigned on plinth and is transformed, on the basis of keeping normal pressure, pressure-keeping functions, realizes that oxygen demand and respiratory curve are real
When dynamic auto trace.
Technical proposal that the invention solves the above-mentioned problems is：
A kind of toy oxygen demand real-time monitoring system with modularization water seal anoxic bottle, including respiration oxygen consumption are real-time
Monitoring device and modularization water seal anoxic bottle for providing closed lab space to toy；
The respiration oxygen consumption real-time monitoring device includes the horizontal ontology being laid with, and the ontology is the Ya Ke of water white transparency
Power plate, and the lower surface of ontology is fitted tightly on smooth barn door；Engraved in ontology capacity slot, oxygen consumption monitoring slot and into
The upper surface of gas guiding groove, ontology is equipped with air inlet and the gas outlet for connectivity module water seal anoxic bottle；Air inlet is logical
Inlet guide slot is crossed to be connected to the capacity slot；One end of the oxygen consumption monitoring slot is connected to capacity slot, goes out described in other end connection
Gas port；The gas outlet is connected by the quick energy converter of height for acquiring respiratory movement data with anoxic bottle；And the Gao Minhuan
Energy device is connected with System of organism signal；
The junction of capacity slot and oxygen consumption monitoring slot is equipped with protrusion, and the protrusion blocks the portion of the entrance of oxygen consumption monitoring slot
Divide cross section；
The length direction for monitoring slot on the upper surface of ontology along oxygen consumption is equipped with scale；Oxygen consumption monitor slot slot bottom on along
The length direction of oxygen consumption monitoring slot is evenly provided with several photo resistance；The photo resistance is sequentially connected in series to form sampling electricity
Resistance, the sampling resistor are connected on a resistance detecting circuit, the output end connection biology of the resistance detecting circuit
Signal acquiring processing system, System of organism signal export animal oxygen demand data according to the data of sampling resistor；
The oxygen consumption monitoring slot has tortuous shape；And oxygen consumption monitoring slot includes several sections of straight troughs being mutually parallel and company
Connect the curved groove of adjacent two straight trough；
Several drainage channels and a return water guide groove are also engraved in ontology, the outlet of drainage channels is led by return water
Slot is connected to capacity slot and inlet guide slot；
Oxygen consumption monitors to be connected to by the guiding device of anti-backflow respectively between slot and the import of each drainage channels, described to lead
Stream device includes baffle and the draining pit that is located on baffle, drains between pit and baffle to have and be connected to oxygen consumption and supervise
Survey the gap of slot and drainage channels；
Draining pit opens up on the body, and baffle is respectively fixedly disposed at curved close to each of drainage channels side
Between slot and drainage channels；It is left end that baffle, which is defined, close to one end of curved groove, is right end close to one end of drainage channels；Water conservancy diversion
The top surface flush of the bottom surface of block and oxygen consumption monitoring slot, the top surface of baffle be gradually ramped up from left end to right end it is oblique
Face, and the left end on the inclined-plane extends at curved groove, the right end on the inclined-plane extends in drainage channels；
The modularization water seal anoxic bottle includes set bottle, bottom cylinder, adapts to bottle and soda lime bottle；
Set bottle can be tipped upside down on to form seal chamber on pedestal, and the bottleneck for covering bottle is connect with the inner thread of bottom cylinder, covers bottle
Bottom of bottle on be equipped with snorkel, one end of snorkel is connected to seal chamber, the other end and respiration oxygen consumption real-time monitoring device
Gas outlet connection；
Adapt to bottle to be located in seal chamber, and several through-holes be laid on the body of adaptation bottle, adapt to the inner cavity of bottle with it is close
Envelope chamber is connected to by the through-hole；
Vacantly setting is adapting in bottle soda lime bottle, and the outside wall surface of soda lime bottle is connect with the inner thread for adapting to bottle,
Soda lime bottle is furnished with cover board, and several grid holes are laid on the bottom of bottle of soda lime bottle and cover board, the inner cavity of soda lime bottle
It is connected to the inner cavity for adapting to bottle by the grid hole.
Further, the groove depth of capacity slot, oxygen consumption monitoring slot and inlet guide slot is 0.5cm.
Further, air inlet and gas outlet are respectively positioned on the upper left side of ontology, and capacity slot is located at the lower right of ontology.
Further, snorkel and the specific connectivity structure of gas outlet are：The side of the ontology is equipped with connection gas outlet
Translation interface, the other side of the ontology is equipped with the anoxic bottle external tapping of connection snorkel, and translation interface and anoxic bottle are external
Mouth passes through high quick energy converter and is connected to.
Further, the gas outlet and translation interface are detachably connected by connecting tube.
Further, straight trough right-to-left horizontal extension on the body, and straight trough and curved groove are vertically connected.
Further, the baffle is wedge type block.
Further, it covers and is equipped with sealing silicagel pad between bottle and bottom cylinder.
Further, the modularization water seal anoxic bottle includes the set bottle that several diameters are identical but height is different, for choosing
With.
Beneficial effects of the present invention are mainly manifested in：
1, test when, the present invention measure oxygen demand fluid column be under horizontality, then fluid column move when, will not be right
Influence is caused stress in modularization water seal anoxic bottle, is solved in conventional oxygen consumption device when mouse breathing consumption oxygen causes in graduated cylinder
Water mouse anoxic in subnormal ambient caused by when being moved into fat tripe suction pipe, to make oxygen demand measurement result generation system
The problem of property error, the present invention effectively increases measurement accuracy.
2, respiration oxygen consumption real-time monitoring device is erect, and oxygen consumption monitoring slot is upward, capacity slot downward, flows into experiment
Water in oxygen consumption monitoring slot can flow to capacity slot by drainage channels, silicone tube, backflash, and draining is very convenient, while
It avoids experiment every time and needs the trouble for adding water.
3, due to the presence of soda lime bottle, ensureing toy energy small range activity while limiting anoxic animal in anoxic bottle
In activity space, avoid the aggravating activities of animal from influencing respiratory movement curve and trace；The presence of built-in ceiling mounting type soda lime,
The carbon dioxide for absorbing the exhalation of anoxic animal, avoids the generation of hypercapnia；In addition, in eminence soda lime be not easy by
The pollution of mouse stool and urine extends the usage time of soda lime, and it is convenient to replace, and mitigates the labor intensity of experiment and improves anoxic reality
The success rate tested.
4, the present invention solves the problems, such as that common classical scarce oxygen consumption device can not monitor breathing and oxygen demand automatically, using light
The principle that pressure oscillation can transmit in closed container caused by electric induction and thorax are shunk solves to use in conventional anoxic experiment
Any time section may be implemented using breathing oxygen consumption real-time monitoring device in the problem of artificial visual method number respiratory rate and oxygen demand
The automatic of hypoxic hypoxia oxygen demand is traced, and the respiratory curve of different type anoxic is traced, and is realized not by analyzing respiratory curve
The comparison of same type anoxic experimental animal respiratory stimulant, mitigates the labor intensity of experiment, improves the accuracy of experimental result.
5, modularization water seal anoxic bottle has done double-encryption on the leakage problem for easily cause the failure of an experiment：It can ensure that experiment
Will not gas leakage and failure, to ensure the leakproofness of anoxic experimental system.
Description of the drawings
Fig. 1 is the structural schematic diagram of the classical device of non-narcotic toy anoxic experiment；
Fig. 2 is the vertical view one for breathing oxygen consumption real-time monitoring device；
Fig. 3 is the internal passage anatomical axis survey one for breathing oxygen consumption real-time monitoring device；
Fig. 4 is the axonometric drawing one for breathing oxygen consumption real-time monitoring device；
Fig. 5 is the axonometric drawing two for breathing oxygen consumption real-time monitoring device；
Fig. 6 is basis《Chinese Pharmacological Bulletin》" dynamic of mouse Whole Oxygen Consumption measures dynamic and surveys by Jiang Zhiwen, Xu Shuxiu etc.
Measurement result calmly "；
Fig. 7 is to carry out the automatic of hypoxic hypoxia oxygen demand using the present invention to trace result；
Fig. 8 is the explosive view one of modularization water seal anoxic bottle of the present invention；
Fig. 9 is the explosive view two of modularization water seal anoxic bottle of the present invention；
Figure 10 is the Structure explosion diagram under modularization water seal anoxic bottle use state.
In figure：01. power interface, 02. power switch, 03. oxygen consumption signaling interface, 04. breath signal interface, 05. capacity
Slot, 06. drainage channels 1, the protrusion of 07. drainage channels 2,08., 09. repair slot, 10~13. repair slots, 14. inlet guide slots,
15. oxygen consumption monitors slot, 16. anoxic bottle external tappings, 17. translation interfaces, 18. gas outlets, 19. air inlets, 20. photo resistance, 21
~22 baffles, 23~24 draining pits, 31. bottoms cylinder, 32. adapt to bottles, 33. soda lime bottles, 34. cover boards, 35~37. sets of bottles,
38. snorkel, 39. silicagel pads.
Specific implementation mode
With reference to attached drawing, a kind of toy oxygen demand real-time monitoring system with modularization water seal anoxic bottle, including breathing
Oxygen demand real-time monitoring device and modularization water seal anoxic bottle for providing closed lab space to toy；
The respiration oxygen consumption real-time monitoring device includes the horizontal ontology being laid with, and the ontology is the Ya Ke of water white transparency
Power plate, and the lower surface of ontology is fitted tightly on smooth barn door；Capacity slot 05, oxygen consumption monitoring slot 15 have been engraved in ontology
It is equipped with air inlet 19 and the gas outlet for connectivity module water seal anoxic bottle with the upper surface of inlet guide slot 14, ontology
18；Air inlet 19 is connected to by inlet guide slot 14 with the capacity slot 05；One end of the oxygen consumption monitoring slot 15 is connected to capacity
Slot 05, the other end are connected to the gas outlet 18；The gas outlet 18 passes through the quick energy converter of height for acquiring respiratory movement data
It is connected with anoxic bottle；And the quick energy converter of height is connected with System of organism signal；
Capacity slot 05 and the junction of oxygen consumption monitoring slot 15 are equipped with protrusion, and the protrusion blocks entering for oxygen consumption monitoring slot 15
The partial cross sectional of mouth；
The length direction for monitoring slot 15 on the upper surface of ontology along oxygen consumption is equipped with scale；Oxygen consumption monitors on the slot bottom of slot 15
The length direction that slot 15 is monitored along oxygen consumption is evenly provided with several photo resistance 20；The photo resistance 20 is sequentially connected in series
Sampling resistor is formed, the sampling resistor is connected on a resistance detecting circuit, the output of the resistance detecting circuit
End connection System of organism signal, System of organism signal export animal oxygen consumption according to the data of sampling resistor
Measure data；
The oxygen consumption monitoring slot 15 has tortuous shape；And oxygen consumption monitoring slot 15 includes several sections of straight troughs being mutually parallel
With the curved groove for connecting adjacent two straight trough；
Several drainage channels 06,07 and a return water guide groove are also engraved in ontology, the outlet of drainage channels 06,07 is equal
It is connected to capacity slot 05 and inlet guide slot 14 by return water guide groove；
Oxygen consumption monitors to be connected by the guiding device of anti-backflow respectively between slot 15 and the import of each drainage channels 06,07
Logical, the guiding device includes baffle and the draining pit 23,24 that is located on baffle, drains pit 23,24 and water conservancy diversion
There is the gap of connection oxygen consumption monitoring slot 15 and drainage channels 06,07 between block 21,22；
Draining pit 23,24 opens up on the body, and baffle 21,22 is respectively fixedly disposed at close to drainage channels 19
Between each curved groove and drainage channels 06,07 of side；It is left end that baffle, which is defined, close to one end of curved groove, close to drainage channels
06,07 one end is right end；The top surface flush of the bottom surface of baffle 21,22 and oxygen consumption monitoring slot 15, the top of baffle 21,22
Face is the inclined-plane gradually ramped up from left end to right end, and the left end on the inclined-plane extends at curved groove, the right side on the inclined-plane
End extends in drainage channels 06,07；
The modularization water seal anoxic bottle includes set bottle 35,36,37, bottom cylinder 31, adaptation bottle 32 and soda lime bottle 33；
Set bottle 35,36,37 can be tipped upside down on to form seal chamber on pedestal 31, and cover the bottleneck and bottom cylinder 31 of bottle 35,36,37
Inner thread connection, cover bottle 35,36,37 bottom of bottle on be equipped with snorkel 38, one end of snorkel 38 is connected to seal chamber,
The other end is connected to the gas outlet 18 of respiration oxygen consumption real-time monitoring device；
It adapts to bottle 32 to be located in seal chamber, and several through-holes is laid on the body of adaptation bottle 32, adapt to the interior of bottle 32
Chamber is connected to seal chamber by the through-hole；
Vacantly setting is adapting in bottle 32 soda lime bottle 33, and the inner wall spiral shell of the outside wall surface of soda lime bottle 33 and adaptation bottle 32
Line connects, and soda lime bottle 33 is furnished with cover board 34, and is laid with several grid holes on the bottom of bottle of soda lime bottle 33 and cover board 34,
The inner cavity of soda lime bottle 34 is connected to by the grid hole with the inner cavity for adapting to bottle 32.
Oxygen consumption monitoring slot 15 is good quick to be shown to the breathing of animal in experiment suitable for making the elongated slot of small cross section
Sensitivity, even if the breathing of mouse can also cause the fluid column of ink to generate apparent movement.
The groove depth of capacity slot 05, oxygen consumption monitoring slot 15 and inlet guide slot 14 is 0.5cm.By prolonged and repeated experiment,
It was found that it is that 1cm is optimal selection that the groove depth of oxygen consumption monitoring slot 15, which takes 0.5cm, width, groove depth is excessively high, and laminar flow can occur for ink,
Slot is wide, and the end face of ink fluid column occurs not move concordantly, influences the accurate recording of oxygen demand；Groove depth is excessively shallow, narrow, can lead
It causes tube chamber long, keeps device excessively huge.
The groove depth of capacity slot 05, oxygen consumption monitoring slot 15 and inlet guide slot 14 is 0.5cm.
Air inlet 19 and gas outlet 18 are respectively positioned on the upper left side of ontology, and capacity slot 05 is located at the lower right of ontology.
Snorkel 38 and the specific connectivity structure of gas outlet 18 are：The side of the ontology is equipped with turning for connection gas outlet 18
Alias 17, the other side of the ontology are equipped with the anoxic bottle external tapping 16 of connection snorkel 38, and translation interface 17 and anoxic
Bottle external tapping 16 is connected to by high quick energy converter.
The gas outlet 18 and translation interface 17 are detachably connected by connecting tube.
Straight trough right-to-left horizontal extension on the body, and straight trough and curved groove are vertically connected.
The baffle is wedge type block.
It covers and is equipped with sealing silicagel pad 39 between bottle 35,36,37 and bottom cylinder 31.
The modularization water seal anoxic bottle includes the set bottle 35,36,37 that several diameters are identical but height is different, for choosing
With.
1, respiration oxygen consumption real-time monitoring device
Barn door is laid on pedestal, and the lateral surface of pedestal is equipped with power interface 01, and photo resistance 20 is connect by power supply
Mouth 01 is connected to extraneous power supply, and power interface 01 is furnished with power switch 02；The lateral surface of pedestal is equipped with oxygen consumption signaling interface
03, photo resistance 20 is connected by oxygen consumption signaling interface 03 with System of organism signal；
The quick tonotransducer of height is connected by breath signal interface 04 with System of organism signal, and is breathed
Signaling interface 04 is located on the lateral surface of pedestal.
The connecting leg is silicone tube.
It is 0.5cm that the width that oxygen consumption monitors slot 15, which is 1cm groove depths, can accommodate the capacity of 45-55ml water in total.
About 5mm*5mm is engraved or is cast between inlet guide slot 14, capacity slot 05, oxygen consumption monitoring slot 15, drainage channels 06,07
Deep and wide rectangle repairs slot 09,10,11,12,13.
Capacity slot 05, oxygen consumption monitoring slot 15, inlet guide slot 14, the drainage channels 06,07 engraved in ontology can both be engraved
It is engraved in body interior, can also be engraved on the lower surface of ontology.But it engraves in body interior, capacity slot 05, oxygen consumption monitoring
Slot 15, inlet guide slot 14, drainage channels 06,07 slot bottom need to be located at the same horizontal plane on；And it engraves in ontology lower surface
When upper, then the slot bottom of each slot was served as by barn door.
When respiration oxygen consumption real-time monitoring device is horizontal positioned, when without outer force effect, 0.5cm thickness in capacity slot 05
Ink will not be flowed under the action of surface tension and adhesiveness, so experiment start before, the water in capacity slot 05 is not
It can be flowed into oxygen consumption monitoring slot 15.Meanwhile protrusion has blocked the partial cross sectional of the entrance of oxygen consumption monitoring slot 15 so that oxygen consumption
The flow resistance for monitoring the ink of slot 15 inlet increases, and further ensures ink before experiment starts in capacity slot 05 not
It can be flowed into oxygen consumption monitoring slot 15.
After experiment starts, ink overcomes the resistance of 15 inlet of oxygen consumption monitoring slot to flow under the traction of the negative pressure of anoxic bottle
Oxygen consumption monitors in slot 15, and flows to gas outlet 18 along oxygen consumption monitoring slot 15.The ink in capacity slot 5 is reduced at this time, air inlet
19 by inlet guide slot 14 to 5 make-up gas of capacity slot.
When experiment (ontology is horizontal positioned), guiding device will be encountered when ink flows through when oxygen consumption monitors the curved groove of slot 15, will be led
Stream block 21,22 can prevent the liquid medium in capacity slot 05 from pouring in down a chimney in oxygen consumption monitoring slot 15.Drain the base opening of pit 23,24
The either flush of place plane and oxygen consumption monitoring slot 15, under the effect of gravity, the liquid medium in oxygen consumption monitoring slot 15 will not be filled out
The gap between draining pit 23,24 and baffle 21,22 is filled, therefore, guiding device not will produce oxygen demand measurement result
It influences.
The length of ink fluid column reflects the oxygen demand of experimental animal, and ink fluid column floods photo resistance 20 and causes sampling electricity
The change in resistance of resistance, oxygen consumption monitor photo resistance 20 regularly arranged under slot 15 and can be used for automatic sensing oxygen demand.Its principle is
When stronger using photo resistance light, resistance value is relatively low, then resistance larger feature when dark, using natural light as transmitting
End, as receiving terminal, oxygen consumption, which monitors, to be changed in slot 15 by 20 resistance value of photo resistance that ink fluid column floods for photo resistance.
Light signal is converted into electric signal using the resistance detecting circuit of electric bridge form, passes through System of organism signal software
The trend curve for showing oxygen demand, by detection signal digitlization.
2, modularization water seal anoxic bottle
Modularization water seal anoxic bottle is a kind of multi-functional anoxic bottle, in conjunction with other mating recording devices of anoxic, Ke Yishi
The duplication and its rescue for answering oxygen demand, hypoxic hypoxia, CO poisoning models are modularization collection on the basis of original various anoxic bottles
It closes.Set of device can carry out different anoxic experiments, yet there are no the use report of multi-functional anoxic bottle.
Application method is similar with traditional anoxic bottle, and 10 minutes before experiment, 25 grams of soda lime is added simultaneously in soda lime bottle 33
Experimental animal is fitted into and adapts in bottle 32 and screw soda lime bottle 33 by the cover board 34 for covering soda lime bottle, and animal is allowed to adapt to bottle
32 endoadaptations 10 minutes or so.Water 20ml is added in bottom cylinder 31, the adaptation bottle 32 equipped with animal is moved into bottom cylinder 31, according to
Different experiments selects different size of set bottle 35,36,37 to be connected with bottom cylinder 31 and turns tight.According to different experiment snorkels 38
It is connected with different types of device, can be used as the use of general anoxia bottle, can also connects breathing oxygen consumption real-time monitoring device and biology is believed
Number acquisition processing system recording respiration curve movement and oxygen demand.
Modularization water seal anoxic bottle is the corollary apparatus for breathing oxygen consumption real-time monitoring device, is the composition portion of the present invention
Point, at present higher medical universities and colleges generally use 120ml wide-mouth bottles as it is common commonly hypoxic hypoxia anoxic bottle,
It cannot be guaranteed the seal of oxygen depletion system in experimentation, soda lime is put in anoxic bottom of bottle portion with gauze wrapped, easily big by mouse
The pollution of urine, prevents soda lime from reusing.
Modularization water seal anoxic bottle has done double-encryption on the leakage problem for easily cause the failure of an experiment：Use screw socket, height
The silicagel pad 09 and water-stop of sealing, can ensure that experiment will not gas leakage and failure can to ensure the leakproofness of anoxic experimental system
Significantly improve the success rate of anoxic experiment.Built-in hanging soda lime bottle 33, makes soda lime be not easy to be polluted by mouse stool and urine,
Extending the usage time of soda lime, addition soda lime is more accurate, convenient and efficient, can mitigate the labor intensity of experiment, hanging
The activity space of the also restricted anoxic experimental animal of soda lime bottle, the automation for being conducive to respiratory movement curve record.
Due to the presence of soda lime bottle 32, is ensureing animal energy small range activity while limiting anoxic animal in anoxic bottle
Activity space, avoid the aggravating activities of animal from influencing respiratory movement curve and trace；The presence of built-in soda lime absorbs scarce
The carbon dioxide of oxygen animal exhalation, avoids the generation of hypercapnia；In addition, the soda lime in eminence is not easy by mouse size
Just pollution extends the usage time of soda lime, and it is convenient to replace, and mitigates the labor intensity of experiment and improves the success of anoxic experiment
Rate.It is docked with the energy converter for connecing breathing oxygen consumption induction measurement slot using this anoxic bottle, you can measure oxygen demand；In addition, due to consumption
Anaerobic environment internal pressure is always held under the negative pressure that the consumption oxygen of the mouse breathing in anoxic bottle generates in oxygen experimentation, such as-
0.5cmH2O, be equivalent to oxygen consumption experiment it is identical as the condition that weary oxygen is tested, therefore, while observing the row of animal during anoxic
To can be used as the observation index of hypoxic hypoxia with color change, change oxygen demand and hypoxic hypoxia in traditional means of experiment
Independent experimental method is needed, animal can be saved or reduces the injury to animal.
3, use step of the invention is：
1) respiration oxygen consumption real-time monitoring device is erected, and capacity slot 05 is downward, oxygen consumption monitors slot 15 upward；Use silica gel
Pipe is connected to gas outlet 18 and translation interface 17；Again from the black that addition was diluted with distilled water into capacity slot 05 in air inlet 19
Ink to water level rises at 0 scale of oxygen consumption monitoring slot 15 (i.e. the inlet of oxygen consumption monitoring slot 15).
2) respiration oxygen consumption real-time monitoring device is horizontal positioned on the table, and breath signal interface 04 is adopted with bio signal
The respective channel for collecting processing system is connected；Then by the respective channel of oxygen consumption signaling interface 03 and System of organism signal
It is connected；Finally power interface 01 is connect with power supply.
3) before testing, 25g soda limes is packed into the soda lime bottle 33 of 50ml and cover cover board 34, by petty action to be tested
Object, which is fitted into, to be adapted in bottle 32, and soda lime bottle 32 is tightened on and is adapted in bottle 32, and animal is allowed to adapt to 32 endoadaptation of bottle 10 minutes
Left and right；Water 20ml is added in bottom cylinder 31, and the adaptation bottle 32 equipped with animal is moved into bottom cylinder 31, is selected according to different experiments
It is connected and is screwed with bottom cylinder 31 with different size of set bottle 35,36,37, and snorkel 39 is monitored in real time by respiration oxygen consumption
The anoxic bottle external tapping 16 of device lateral surface is connected to.
4) startup power supply switch 02, experiment starts.When mouse breathing consumption oxygen in anoxic bottle generates negative pressure, such as
0.5cmH2O columns, the ink in capacity slot 05 can fill up consumption due to the effect of negative pressure to the 15 direction flowing of oxygen consumption monitoring slot
Oxygen capacity makes the pressure in respiration oxygen consumption real-time monitoring device be constant all the time in -0.5cm H2O columns.Mouse breathes out titanium dioxide
Carbon is absorbed by the soda lime placed in consumption anoxic bottle again, and therefore, capacity slot 05 flows to the milliliter of ink in oxygen consumption monitoring slot 15
Number is the oxygen demand of mouse (scale that personal monitoring can monitor 15 surface of slot by oxygen consumption reads oxygen demand)；
When the dilution ink in capacity slot 05 is flowed to oxygen consumption monitoring slot 15, you can flood photo resistance 20, shelter from
Original light impinged upon in photo resistance 20, the ink for flowing into oxygen consumption monitoring slot 15 is more, and the photo resistance 20 being blocked is more
More, resistance detecting circuit can filter out useful signal and application signal control voltage output, by biochips diagnosis
System traces out oxygen demand automatically.
When mouse breathing, the expansion and contraction of lung cause to breathe the pressure oscillation in oxygen consumption real-time monitoring device, pass through height
Quick energy converter is transformed into electric signal and is transmitted to RM6240 System of organism signal, and respiratory curve is traced out with direct current signal,
Accurately dynamic monitoring the pressure in oxygen consumption real-time monitoring device, respiratory rate and amplitude can be breathed, realize anoxic experiment mice
The automatic monitoring of respiratory rate, amplitude and pressure, avoids error caused by personal monitoring.
5) to be tested again, by the present invention erect, and oxygen consumption monitoring slot 15 upward, capacity slot 05 downward, oxygen consumption
Water in monitoring slot 15 can flow to capacity slot 05 by draining the gap between pit 23,24 and drainage channels 06,07.It drains
After flowing into the water in oxygen consumption monitoring slot 15 in experiment, then oxygen consumption real-time monitoring device will be breathed and be horizontally arranged on the table, you can
It is tested again, avoids experiment every time and need the trouble for adding water.
2, experimental result of the invention and advantage explanation
According to《Chinese Pharmacological Bulletin》Jiang Zhiwen, Xu Shuxiu's etc. " dynamic of mouse Whole Oxygen Consumption measures dynamic and measures "
As a result the measurement result (see Fig. 7) of oxygen demand is carried out (see Fig. 6) and using the present invention to be obtained：The oxygen consumption experiment of different experiments room
The capacity of used anoxic bottle is different, and there is a notable difference in the oxygen consumption time-to-live, but oxygen demand curve be in one first rise after under
The two-way curve of drop, oxygen consumption occurs mainly in the front half section time, such as is carried out in oxygen demand measurement first 12 minutes using the present invention
The 71% of total oxygen demand is accounted for, later half time oxygen demand accounts for the 21% of total amount, and is in uniform descent；When illustrating to measure front half section
Between can be obtained the oxygen demand result of difference (obtain) for changing meaning.
Due to present invention System of organism signal recording respiration curve, when can acquire any during anoxic
Between section respiratory rate, amplitude (ventilatory capacity) and oxygen demand, overcome old device visual method number respiratory rate (mouse breathing it is weak and
Soon,>200 times/min, the problem of can not accurately obtaining data on time, even if obtaining the data of respiratory rate, it can not correct and exhale
Inhale the problem of frequency is not necessarily positively correlated with respiratory stimulant) and oxygen demand, it can change routine by promoting the use of energy invention
The way of anoxic operating limit oxygen consumption, the way for making time oxygen consumption rate or minute oxygen demand into obtain experimental result, avoid pole
Limit oxygen consumption murders animal.
The present invention breaks through the recording mode of existing hypoxia device on the basis of succession classical characteristic, it may be said that is that collection is permanent
Pressure, dynamic monitoring respiratory rate, amplitude, oxygen demand and anaerobic environment pressure are the systematization anoxic experimental tool of the whole body, are overcome
The problem of old device in background technology reads oxygen demand data with visual method, and data can not be restored by missing acquisition time just,
And alleviate labor intensity, and it is cheap, make it is simple, be suitable for teaching and scientific research.
Compared with old device, the present invention has following advantage：
The present invention records dynamic oxygen demand with System of organism signal, can acquire any time during anoxic
The oxygen consumption rate of section overcomes old device visual method to read oxygen demand data, misses acquisition time and can not just restore data
Problem, and alleviate labor intensity；The way that conventional anoxic operating limit oxygen consumption can be changed using the present invention makes time consumption into
The way of oxygen rate or minute oxygen demand obtains experimental result, and Limiting Oxygen is avoided to murder animal；It is of particular importance that this
Horizontal fluid column is used when invention experiment, does not interfere with experimental precision.
Content described in this specification embodiment is only enumerating to the way of realization of inventive concept, protection of the invention
Range is not construed as being only limitted to the concrete form that embodiment is stated, protection scope of the present invention also includes art technology
Personnel according to present inventive concept it is conceivable that equivalent technologies mean.
Claims (9)
1. a kind of toy oxygen demand real-time monitoring system with modularization water seal anoxic bottle, it is characterised in that：Including breathing
Oxygen demand real-time monitoring device and modularization water seal anoxic bottle for providing closed lab space to toy；
The respiration oxygen consumption real-time monitoring device includes the horizontal ontology being laid with, and the ontology is the acrylic of water white transparency
Plate, and the lower surface of ontology is fitted tightly on smooth barn door；Capacity slot, oxygen consumption monitoring slot and air inlet have been engraved in ontology
The upper surface of guiding groove, ontology is equipped with air inlet and the gas outlet for connectivity module water seal anoxic bottle；Air inlet passes through
Inlet guide slot is connected to the capacity slot；One end of the oxygen consumption monitoring slot is connected to capacity slot, and the other end is connected to the outlet
Mouthful；The gas outlet is connected by the quick energy converter of height for acquiring respiratory movement data with anoxic bottle；And the quick transducing of height
Device is connected with System of organism signal；
The junction of capacity slot and oxygen consumption monitoring slot is equipped with protrusion, and the protrusion blocks the part cross of the entrance of oxygen consumption monitoring slot
Section；
The length direction for monitoring slot on the upper surface of ontology along oxygen consumption is equipped with scale；Oxygen consumption monitors on the slot bottom of slot along oxygen consumption
The length direction of monitoring slot is evenly provided with several photo resistance；The photo resistance is sequentially connected in series to form sampling resistor,
The sampling resistor is connected on a resistance detecting circuit, and the output end of the resistance detecting circuit connects bio signal
Acquisition processing system, System of organism signal export animal oxygen demand data according to the data of sampling resistor；
The oxygen consumption monitoring slot has tortuous shape；And oxygen consumption monitoring slot includes several sections of straight troughs being mutually parallel and connection phase
The curved groove of adjacent two straight troughs；
Several drainage channels and a return water guide groove are also engraved in ontology, the outlet of drainage channels is connected by return water guide groove
Logical capacity slot and inlet guide slot；
Oxygen consumption monitors to be connected to by the guiding device of anti-backflow respectively between slot and the import of each drainage channels, the water conservancy diversion dress
It sets including baffle and the draining pit being located on baffle, has between draining pit and baffle and be connected to oxygen consumption monitoring slot
With the gap of drainage channels；
Draining pit opens up on the body, baffle be respectively fixedly disposed at each curved groove close to drainage channels side with
Between drainage channels；It is left end that baffle, which is defined, close to one end of curved groove, is right end close to one end of drainage channels；Baffle
The top surface flush of bottom surface and oxygen consumption monitoring slot, the top surface of baffle is the inclined-plane gradually ramped up from left end to right end, and
The left end on the inclined-plane extends at curved groove, and the right end on the inclined-plane extends in drainage channels；
The modularization water seal anoxic bottle includes set bottle, bottom cylinder, adapts to bottle and soda lime bottle；
Set bottle can be tipped upside down on to form seal chamber on pedestal, and the bottleneck for covering bottle is connect with the inner thread of bottom cylinder, covers the bottle of bottle
Snorkel is equipped on bottom, one end of snorkel is connected to seal chamber, and the other end goes out with respiration oxygen consumption real-time monitoring device
Gas port is connected to；
It adapts to bottle to be located in seal chamber, and several through-holes is laid on the body of adaptation bottle, adapt to inner cavity and the seal chamber of bottle
It is connected to by the through-hole；
Vacantly setting is adapting in bottle soda lime bottle, and the outside wall surface of soda lime bottle is connect with the inner thread for adapting to bottle, sodium stone
Grey bottle is furnished with cover board, and several grid holes are laid on the bottom of bottle of soda lime bottle and cover board, and the inner cavity of soda lime bottle passes through
The grid hole is connected to the inner cavity for adapting to bottle.
2. a kind of toy oxygen demand real-time monitoring system with modularization water seal anoxic bottle as described in claim 1,
It is characterized in that：The groove depth of capacity slot, oxygen consumption monitoring slot and inlet guide slot is 0.5cm.
3. a kind of toy oxygen demand real-time monitoring system with modularization water seal anoxic bottle as claimed in claim 2,
It is characterized in that：Air inlet and gas outlet are respectively positioned on the upper left side of ontology, and capacity slot is located at the lower right of ontology.
4. a kind of toy oxygen demand real-time monitoring system with modularization water seal anoxic bottle as claimed in claim 3,
It is characterized in that：Snorkel and the specific connectivity structure of gas outlet are：The conversion that the side of the ontology is equipped with connection gas outlet connects
Mouthful, the other side of the ontology is equipped with the anoxic bottle external tapping of connection snorkel, and translation interface and anoxic bottle external tapping pass through
High quick energy converter connection.
5. a kind of toy oxygen demand real-time monitoring system with modularization water seal anoxic bottle as claimed in claim 4,
It is characterized in that：The gas outlet and translation interface are detachably connected by connecting tube.
6. a kind of toy oxygen demand real-time monitoring system with modularization water seal anoxic bottle as claimed in claim 5,
It is characterized in that：Straight trough right-to-left horizontal extension on the body, and straight trough and curved groove are vertically connected.
7. a kind of toy oxygen demand real-time monitoring system with modularization water seal anoxic bottle as claimed in claim 6,
It is characterized in that：The baffle is wedge type block.
8. a kind of toy oxygen demand real-time monitoring system with modularization water seal anoxic bottle as claimed in claim 7,
It is characterized in that：It covers and is equipped with sealing silicagel pad between bottle and bottom cylinder.
9. a kind of toy oxygen demand real-time monitoring system with modularization water seal anoxic bottle as claimed in claim 8,
It is characterized in that：The modularization water seal anoxic bottle includes the set bottle that several diameters are identical but height is different, for selecting.
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| CN201810149745.2A CN108272455B (en) | 2018-02-13 | 2018-02-13 | Real-time oxygen consumption monitoring system for small animals with modularized water-seal anoxic bottles |
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| CN201810149745.2A CN108272455B (en) | 2018-02-13 | 2018-02-13 | Real-time oxygen consumption monitoring system for small animals with modularized water-seal anoxic bottles |
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| CN110136835A (en) * | 2019-05-29 | 2019-08-16 | 西南交通大学 | The determination method, apparatus and electronic equipment of physical labor intensity |
| CN110623670A (en) * | 2019-08-16 | 2019-12-31 | 浙江大学 | Split type respiration oxygen consumption real-time monitoring device |
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| CN108272455B (en) | 2020-05-19 |
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