CN110613063A - Prawn fermented feed and preparation method and application thereof - Google Patents
Prawn fermented feed and preparation method and application thereof Download PDFInfo
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- CN110613063A CN110613063A CN201910986572.4A CN201910986572A CN110613063A CN 110613063 A CN110613063 A CN 110613063A CN 201910986572 A CN201910986572 A CN 201910986572A CN 110613063 A CN110613063 A CN 110613063A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 51
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- 235000018262 Arachis monticola Nutrition 0.000 claims abstract description 13
- 235000019733 Fish meal Nutrition 0.000 claims abstract description 13
- 235000019779 Rapeseed Meal Nutrition 0.000 claims abstract description 13
- 235000021323 fish oil Nutrition 0.000 claims abstract description 13
- 239000004467 fishmeal Substances 0.000 claims abstract description 13
- 229910052500 inorganic mineral Inorganic materials 0.000 claims abstract description 13
- 235000012054 meals Nutrition 0.000 claims abstract description 13
- 239000011707 mineral Substances 0.000 claims abstract description 13
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- 239000004456 rapeseed meal Substances 0.000 claims abstract description 13
- 239000000126 substance Substances 0.000 claims abstract description 13
- 235000013343 vitamin Nutrition 0.000 claims abstract description 13
- 229940088594 vitamin Drugs 0.000 claims abstract description 13
- 229930003231 vitamin Natural products 0.000 claims abstract description 13
- 239000011782 vitamin Substances 0.000 claims abstract description 13
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 11
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 9
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 8
- 241000193388 Bacillus thuringiensis Species 0.000 claims abstract description 8
- 238000000855 fermentation Methods 0.000 claims abstract description 8
- 230000004151 fermentation Effects 0.000 claims abstract description 8
- 229940097012 bacillus thuringiensis Drugs 0.000 claims abstract description 7
- 238000002156 mixing Methods 0.000 claims description 22
- 229940088598 enzyme Drugs 0.000 claims description 15
- 238000003756 stirring Methods 0.000 claims description 15
- -1 compound vitamin Chemical class 0.000 claims description 10
- 239000008187 granular material Substances 0.000 claims description 8
- 238000004321 preservation Methods 0.000 claims description 6
- 239000002245 particle Substances 0.000 claims description 4
- 108090000145 Bacillolysin Proteins 0.000 claims description 2
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 2
- 108010059892 Cellulase Proteins 0.000 claims description 2
- 102000035092 Neutral proteases Human genes 0.000 claims description 2
- 108091005507 Neutral proteases Proteins 0.000 claims description 2
- 229940106157 cellulase Drugs 0.000 claims description 2
- 235000015097 nutrients Nutrition 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 3
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- 210000002700 urine Anatomy 0.000 abstract 1
- 230000004083 survival effect Effects 0.000 description 19
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- 235000021053 average weight gain Nutrition 0.000 description 12
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- OHVLMTFVQDZYHP-UHFFFAOYSA-N 1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)-2-[4-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidin-5-yl]piperazin-1-yl]ethanone Chemical compound N1N=NC=2CN(CCC=21)C(CN1CCN(CC1)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)=O OHVLMTFVQDZYHP-UHFFFAOYSA-N 0.000 description 3
- KZEVSDGEBAJOTK-UHFFFAOYSA-N 1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)-2-[5-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidin-5-yl]-1,3,4-oxadiazol-2-yl]ethanone Chemical compound N1N=NC=2CN(CCC=21)C(CC=1OC(=NN=1)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)=O KZEVSDGEBAJOTK-UHFFFAOYSA-N 0.000 description 3
- LDXJRKWFNNFDSA-UHFFFAOYSA-N 2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)-1-[4-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidin-5-yl]piperazin-1-yl]ethanone Chemical compound C1CN(CC2=NNN=C21)CC(=O)N3CCN(CC3)C4=CN=C(N=C4)NCC5=CC(=CC=C5)OC(F)(F)F LDXJRKWFNNFDSA-UHFFFAOYSA-N 0.000 description 3
- JQMFQLVAJGZSQS-UHFFFAOYSA-N 2-[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]piperazin-1-yl]-N-(2-oxo-3H-1,3-benzoxazol-6-yl)acetamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)N1CCN(CC1)CC(=O)NC1=CC2=C(NC(O2)=O)C=C1 JQMFQLVAJGZSQS-UHFFFAOYSA-N 0.000 description 3
- YLZOPXRUQYQQID-UHFFFAOYSA-N 3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)-1-[4-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidin-5-yl]piperazin-1-yl]propan-1-one Chemical compound N1N=NC=2CN(CCC=21)CCC(=O)N1CCN(CC1)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F YLZOPXRUQYQQID-UHFFFAOYSA-N 0.000 description 3
- 241000530454 Litopenaeus schmitti Species 0.000 description 3
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 3
- 239000006041 probiotic Substances 0.000 description 3
- 235000018291 probiotics Nutrition 0.000 description 3
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000000645 desinfectant Substances 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 230000000529 probiotic effect Effects 0.000 description 2
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 201000006549 dyspepsia Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 150000003242 quaternary ammonium salts Chemical class 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Marine Sciences & Fisheries (AREA)
- Physiology (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Environmental Sciences (AREA)
- Biochemistry (AREA)
- Insects & Arthropods (AREA)
- Microbiology (AREA)
- Birds (AREA)
- Inorganic Chemistry (AREA)
- Botany (AREA)
- Mycology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Sustainable Development (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Abstract
The invention provides a prawn fermented feed and a preparation method and application thereof, belonging to the technical field of aquatic feeds. The preparation raw materials of the fermented feed comprise: fish meal, peanut meal, rapeseed meal, fish oil, compound vitamins, compound minerals, a strain compound preparation and a compound enzyme preparation. The strain composite preparation comprises the following components: bacillus subtilis DB005, bacillus thuringiensis XW008 and bacillus licheniformis XW 015. The preparation method of the feed is simple, and the raw materials are cheap and easily available, so that the feed is low in manufacturing cost and suitable for large-scale popularization and application. Secondly, the raw materials for preparing the feed are rich in nutrient substances, and the protein and the cellulose in the raw materials can be decomposed through the fermentation of the reasonable strains and the complex enzyme preparation, so that the utilization rate of the nutrient substances of the feed and the growth speed of the prawns can be improved. In addition, the fermented feed is easier to digest and absorb, so that the emission of excrement and urine can be reduced, and the pollution to the culture environment is reduced.
Description
Technical Field
The invention relates to the technical field of aquatic feeds, in particular to a prawn fermented feed and a preparation method and application thereof.
Background
The south America white shrimps are commonly called white shrimps, and the shape of the white shrimps is exactly like Chinese shrimps. The penaeus vannamei boone has the advantages of delicious meat, high meat yield, wide salt range and high temperature resistance, and the seedlings of the penaeus vannamei boone can grow into adults with the body length of 24cm after being cultured for more than 100 days. The penaeus vannamei boone is mainly distributed in the pacific coast tropical water area in the western United states, is distributed from gulf of Mexico to the middle part of Peru, is more concentrated in the sea area near the Ecuador, and is one of three excellent varieties with the highest world culture yield.
Since 1988, the introduction, adaptive culture and large-area popularization of penaeus vannamei boone have been developed in China. After 2000, the culture scale of the penaeus vannamei boone is enlarged year by year. In 2013, the yield of the penaeus vannamei boone in China reaches more than 130 million tons. Since then, the yield of the penaeus vannamei boone in China is reduced due to frequent diseases, but the yield is still maintained to be about 100 million tons every year.
With the development of the scale and industry of the penaeus vannamei boone culture, a plurality of problems such as indigestion, low feed utilization rate, obviously reduced growth speed, frequent diseases and the like are also generated. At present, in the aspect of disease prevention and treatment of the penaeus vannamei boone, the penaeus vannamei boone is mainly prevented and treated by using a large amount of disinfectants such as antibiotics, quaternary ammonium salt and iodine preparations, so that the ecological balance of the culture environment is seriously influenced, and the antibiotics and the disinfectants are left in the bodies of the penaeus vannamei boone and harm the health of human beings.
Disclosure of Invention
The invention aims to provide the prawn fermented feed which is easy to digest, has high utilization rate of nutrient substances and can effectively inhibit pathogenic bacteria; the second purpose of the present invention is to provide a method for producing the fermented feed; the third purpose of the invention is to provide an application method of the fermented feed.
In order to achieve the purpose, the invention provides a prawn fermentation feed, which comprises the following preparation raw materials: fish meal, peanut meal, rapeseed meal, fish oil, compound vitamins, compound minerals, a strain compound preparation and a compound enzyme preparation.
Preferably, the fermented feed comprises the following raw materials in parts by weight: 15-20 parts of fish meal, 40-60 parts of peanut meal, 20-30 parts of rapeseed meal, 2-10 parts of fish oil, 5-10 parts of compound vitamin, 5-10 parts of compound mineral, 10-30 parts of strain compound preparation and 1-3 parts of compound enzyme preparation.
Preferably, the components of the strain compound preparation comprise: bacillus subtilis DB005, bacillus thuringiensis XW008 and bacillus licheniformis XW 015.
Preferably, the strain compound preparation comprises the following components in percentage by weight: b, B.subtilis DB 005: bacillus thuringiensis XW 008: bacillus licheniformis XW015=3:1: 1.
Preferably, the Bacillus subtilis DB005 (B.subtilis)Bacillus subtilis) The preservation number is CGMCC No. 15646;
bacillus thuringiensis XW008(Bacillus thuringensis) The preservation number is CGMCC No. 15647;
bacillus licheniformis XW015 (Bacillus licheniformis) The preservation number is CGMCC number 15648.
Preferably, the compound enzyme preparation comprises the following components in percentage by weight: neutral protease: cellulase =1: 1.
In addition, the invention also provides a preparation method of the prawn fermentation feed, which comprises the following steps:
(1) fermenting raw materials: uniformly mixing fish meal, peanut meal, rapeseed meal, fish oil, a strain composite preparation and a composite enzyme preparation in parts by weight, and fermenting to obtain a feed A;
(2) mixing raw materials: adding the compound vitamin and the compound mineral substance into the feed A according to the parts by weight, and uniformly stirring to obtain a feed B;
(3) and (3) granulating: and granulating the feed B by using granulating equipment to obtain the fermented feed.
Preferably, the temperature of the fermentation in step (1) is 25-35 ℃.
Preferably, the fermentation time in step (1) is 12-48 hours.
Preferably, the particle size of the particles in the step (3) is 1.2mm, and the length of the particles is 1.8 mm.
In addition, the invention also provides application of the prawn fermented feed, and the fermented feed is directly fed to the prawns.
The beneficial effects of the invention include:
1. the preparation method of the feed is simple, and the raw materials are cheap and easily available, so that the feed is low in manufacturing cost and suitable for large-scale popularization and application.
2. The feed has the advantages that the preparation raw materials are reasonably matched, the nutrient substances are rich, the protein and the cellulose in the raw materials can be fully fermented under the synergistic effect of the strain compound preparation and the complex enzyme preparation which are composed of the probiotics in a proper proportion, and the fermented fragrance of the fermented feed can promote the appetite of the prawns, so that the nutrient substance utilization rate of the feed and the growth speed of the prawns can be improved.
3. The fermented feed is easier to digest and absorb, so that the excrement discharge of the prawns is reduced, and the pollution to the culture environment is reduced. And secondly, the probiotic strains used by the invention can decompose residual baits in the culture environment, so that the pollution of the residual baits to the culture environment is effectively reduced, the morbidity probability of the prawns is reduced, and the survival rate of the prawns is improved.
4. The probiotic strains used in the feed can effectively inhibit pathogenic bacteria, improve the intestinal flora and immunity of the penaeus vannamei boone, reduce the morbidity of the penaeus vannamei boone and improve the survival rate of the penaeus vannamei boone.
Detailed Description
The present invention is further illustrated by the following examples, which are intended to be purely exemplary of the invention and are not intended to be limiting.
Example 1
1. Fermenting raw materials: mixing 20 parts of fish meal, 50 parts of peanut meal, 30 parts of rapeseed meal, 2 parts of fish oil, 30 parts of a strain composite preparation and 1 part of a composite enzyme preparation, and stirring; after being stirred evenly, the mixture is placed at 25 ℃ and fermented for 48 hours to obtain the feed A.
2. Mixing raw materials: and mixing the feed A, 5 parts of compound vitamin and 5 parts of compound mineral substance, and uniformly stirring to obtain a feed B.
3. And (3) granulating: and (3) preparing the feed B into granules with the diameter of 1.2mm and the length of 1.8mm by granulation equipment to obtain the fermented feed 1.
4. Feeding: 1200 prawns with the size of 5g are selected, are randomly and evenly distributed into 3 culture ponds, and are fed by using the fermented feed 1, wherein the feeding period is 55 days. After feeding, the average weight gain rate and survival rate of the prawns in the 3 culture ponds are detected.
Example 2
1. Fermenting raw materials: mixing 15 parts of fish meal, 55 parts of peanut meal, 20 parts of rapeseed meal, 10 parts of fish oil, 25 parts of a strain composite preparation and 1 part of a composite enzyme preparation, and stirring; after being stirred evenly, the mixture is placed at 25 ℃ and fermented for 48 hours to obtain the feed A.
2. Mixing raw materials: and mixing the feed A, 5 parts of compound vitamin and 5 parts of compound mineral substance, and uniformly stirring to obtain a feed B.
3. And (3) granulating: and (3) preparing the feed B into granules with the diameter of 1.2mm and the length of 1.8mm by using granulating equipment to obtain the fermented feed 2.
4. Feeding: 1200 prawns with the same size are selected, are randomly and evenly distributed into 3 culture ponds, and are fed by using the fermented feed 2, wherein the feeding period is 55 days. After feeding, the average weight gain rate and survival rate of the prawns in the 3 culture ponds are detected.
Example 3
1. Fermenting raw materials: mixing 20 parts of fish meal, 50 parts of peanut meal, 30 parts of rapeseed meal, 5 parts of fish oil, 20 parts of a strain composite preparation and 2 parts of a complex enzyme preparation, and stirring; after being stirred evenly, the mixture is placed at 30 ℃ and fermented for 24 hours to obtain the feed A.
2. Mixing raw materials: and mixing the feed A, 5 parts of compound vitamin and 5 parts of compound mineral substance, and uniformly stirring to obtain a feed B.
3. And (3) granulating: and (3) granulating the feed B into granules with the diameter of 1.2mm and the length of 1.8mm by using granulating equipment to obtain the fermented feed 3.
4. Feeding: 1200 prawns with the same size are selected, are randomly and evenly distributed into 3 culture ponds, and are fed by using the fermented feed 3, wherein the feeding period is 55 days. After feeding, the average weight gain rate and survival rate of the prawns in the 3 culture ponds are detected.
Example 4
1. Fermenting raw materials: mixing 15 parts of fish meal, 60 parts of peanut meal, 20 parts of rapeseed meal, 10 parts of fish oil, 15 parts of a strain composite preparation and 3 parts of a composite enzyme preparation, and stirring; after being stirred evenly, the mixture is placed at 30 ℃ and fermented for 24 hours to obtain the feed A.
2. Mixing raw materials: and mixing the feed A, 10 parts of compound vitamin and 10 parts of compound mineral substance, and uniformly stirring to obtain a feed B.
3. And (3) granulating: and (3) granulating the feed B into granules with the diameter of 1.2mm and the length of 1.8mm by using granulating equipment to obtain the fermented feed 4.
4. Feeding: 1200 prawns with the same size are selected, are randomly and evenly distributed into 3 culture ponds, and are fed by fermented feed 4, wherein the feeding period is 55 days. After feeding, the average weight gain rate and survival rate of the prawns in the 3 culture ponds are detected.
Example 5
1. Fermenting raw materials: mixing 20 parts of fish meal, 50 parts of peanut meal, 25 parts of rapeseed meal, 5 parts of fish oil, 15 parts of a strain composite preparation and 3 parts of a complex enzyme preparation, and stirring; after being stirred evenly, the mixture is placed at 35 ℃ and fermented for 12 hours to obtain the feed A.
2. Mixing raw materials: and mixing the feed A, 10 parts of compound vitamin and 10 parts of compound mineral substance, and uniformly stirring to obtain a feed B.
3. And (3) granulating: and (3) preparing the feed B into granules with the diameter of 1.2mm and the length of 1.8mm by using granulating equipment to obtain the fermented feed 5.
4. Feeding: 1200 prawns with the same size are selected, are randomly and evenly distributed into 3 culture ponds, and are fed by using fermented feed 5, wherein the feeding period is 55 days. After feeding, the average weight gain rate and survival rate of the prawns in the 3 culture ponds are detected.
Example 6
1. Fermenting raw materials: mixing 15 parts of fish meal, 60 parts of peanut meal, 20 parts of rapeseed meal, 5 parts of fish oil, 10 parts of a strain composite preparation and 3 parts of a complex enzyme preparation, and stirring; uniformly stirring, and fermenting at 35 ℃ for 12 hours to obtain the feed A.
2. Mixing raw materials: and mixing the feed A, 10 parts of compound vitamin and 10 parts of compound mineral substance, and uniformly stirring to obtain a feed B.
3. And (3) granulating: and (3) preparing the feed B into granules with the diameter of 1.2mm and the length of 1.8mm by using granulating equipment to obtain the fermented feed 6.
4. Feeding: 1200 prawns with the same size are selected, are randomly and evenly distributed into 3 culture ponds, and are fed by fermented feed 6, wherein the feeding period is 55 days. After feeding, the average weight gain rate and survival rate of the prawns in the 3 culture ponds are detected.
Control group 1
1200 prawns with the same size are selected, randomly and evenly distributed into 3 culture ponds, and fed by using a certain brand of commercial common feed 1, the other conditions are completely the same as those in examples 1-6, and the feeding period is 55 days. After feeding, the average weight gain rate and survival rate of the prawns in the 3 culture ponds are detected.
Control group 2
1200 prawns with the same size are selected, randomly and evenly distributed into 3 culture ponds, and fed by using a certain brand of commercial common feed 2, the other conditions are completely the same as those in examples 1-6, and the feeding period is 55 days. After feeding, the average weight gain rate and survival rate of the prawns in the 3 culture ponds are detected. The beneficial effects of the present invention are further illustrated below in conjunction with experimental data:
1. results of the weight gain test
Total weight of seedlings put/kg | Total final weight/kg | Weight gain/kg | Weight gain% | Average rate of weight gain/%) | |
Example 1 | 2 | 7.8 | 5.8 | 290 | |
Example 1 | 2 | 7.9 | 5.9 | 295 | 295 |
Example 1 | 2 | 8.0 | 6.0 | 300 | |
Example 2 | 2 | 7.8 | 5.8 | 290 | |
Example 2 | 2 | 7.7 | 5.7 | 285 | 290 |
Example 2 | 2 | 7.9 | 5.9 | 295 | |
Example 3 | 2 | 8.2 | 6.2 | 310 | |
Example 3 | 2 | 8.6 | 6.6 | 330 | 312 |
Example 3 | 2 | 7.9 | 5.9 | 295 | |
Example 4 | 2 | 8.2 | 6.2 | 310 | |
Example 4 | 2 | 8.0 | 6.0 | 300 | 296 |
Example 4 | 2 | 7.6 | 5.6 | 280 | |
Example 5 | 2 | 8.4 | 6.4 | 320 | |
Example 5 | 2 | 7.8 | 5.8 | 290 | 305 |
Example 5 | 2 | 8.1 | 6.1 | 305 | |
Example 6 | 2 | 8.2 | 6.2 | 310 | |
Example 6 | 2 | 8.0 | 6.0 | 300 | 302 |
Example 6 | 2 | 7.9 | 5.9 | 295 | |
Control group 1 | 2 | 7.3 | 5.3 | 265 | |
Control group 1 | 2 | 7.5 | 5.5 | 275 | 267 |
Control group 1 | 2 | 7.2 | 5.2 | 260 | |
Control group 2 | 2 | 7.0 | 5.0 | 250 | |
Control group 2 | 2 | 6.9 | 4.9 | 245 | 253 |
Control group 2 | 2 | 7.3 | 5.3 | 265 |
2. Survival rate experimental results
Initial prawn number | Final prawn number | Survival rate/% | Average survival rate/% | |
Example 1 | 400 | 384 | 96 | |
Example 1 | 400 | 384 | 96 | 96 |
Example 1 | 400 | 388 | 97 | |
Example 2 | 400 | 370 | 93 | |
Example 2 | 400 | 385 | 96 | 94 |
Example 2 | 400 | 378 | 95 | |
Example 3 | 400 | 378 | 95 | |
Example 3 | 400 | 380 | 95 | 94 |
Example 3 | 400 | 373 | 93 | |
Example 4 | 400 | 370 | 93 | |
Example 4 | 400 | 382 | 96 | 93 |
Example 4 | 400 | 366 | 92 | |
Example 5 | 400 | 386 | 97 | |
Example 5 | 400 | 384 | 96 | 97 |
Example 5 | 400 | 390 | 98 | |
Example 6 | 400 | 375 | 94 | |
Example 6 | 400 | 380 | 95 | 95 |
Example 6 | 400 | 387 | 97 | |
Control group 1 | 400 | 362 | 91 | |
Control group 1 | 400 | 360 | 90 | 90 |
Control group 1 | 400 | 356 | 89 | |
Control group 2 | 400 | 350 | 88 | |
Control group 2 | 400 | 355 | 89 | 89 |
Control group 2 | 400 | 365 | 91 |
The results show that compared with the control group 1, the average weight gain rate of the prawns fed with the feed of the invention is improved by 23-45 percent, and the average survival rate is improved by 3-7 percent. Compared with the control group 2, the average weight gain rate of the prawns fed with the feed is improved by 37-59 percent, and the average survival rate of the prawns is improved by 4-8 percent.
The weight gain effect of the embodiment 3 is the best, and compared with the control group 1, the average weight gain rate of the prawns is improved by 45 percentage points; compared with the control group 2, the average weight gain rate of the prawns is improved by 59 percent. Example 5 is the best in improving the survival rate of the prawns, and the average survival rate of the prawns is improved by 7 percent compared with the control group 1; compared with the control group 2, the average survival rate of the prawns is improved by 8 percent.
In conclusion, the feed of the invention can improve the growth speed of the prawns and the survival rate of the prawns.
Claims (10)
1. The fermented feed for prawns is characterized by comprising the following preparation raw materials: fish meal, peanut meal, rapeseed meal, fish oil, compound vitamins, compound minerals, a strain compound preparation and a compound enzyme preparation.
2. The fermented feed for prawns according to claim 1, wherein the fermented feed is prepared from the following raw materials in parts by weight: 15-20 parts of fish meal, 40-60 parts of peanut meal, 20-30 parts of rapeseed meal, 2-10 parts of fish oil, 5-10 parts of compound vitamin, 5-10 parts of compound mineral, 10-30 parts of strain compound preparation and 1-3 parts of compound enzyme preparation.
3. The fermented feed for prawns according to claim 1 or 2, wherein the strain compound preparation comprises the following components: bacillus subtilis DB005, bacillus thuringiensis XW008 and bacillus licheniformis XW 015.
4. The fermented feed for prawns according to claim 3, wherein the strain compound preparation comprises the following components in parts by weight: b, B.subtilis DB 005: bacillus thuringiensis XW 008: bacillus licheniformis XW015=3:1: 1.
5. The fermented feed for prawns according to claim 4, wherein the bacillus subtilis DB005 ((Bacillus subtilis))Bacillus subtilis) The preservation number is CGMCC No. 15646;
bacillus thuringiensis XW008 (B.thuringiensis)Bacillus thuringensis) The preservation number is CGMCC No. 15647;
bacillus licheniformis XW015 (Bacillus licheniformis) The preservation number is CGMCC number 15648.
6. The fermented feed for prawns according to claim 1 or 2, characterized in that the compound enzyme preparation comprises the following components in parts by weight: neutral protease: cellulase =1: 1.
7. A preparation method of a prawn fermentation feed is characterized by comprising the following steps:
(1) fermenting raw materials: uniformly mixing fish meal, peanut meal, rapeseed meal, fish oil, a strain composite preparation and a composite enzyme preparation in parts by weight, and fermenting to obtain a feed A;
(2) mixing raw materials: adding the compound vitamin and the compound mineral substance into the feed A according to the parts by weight, and uniformly stirring to obtain a feed B;
(3) and (3) granulating: and granulating the feed B by using granulating equipment to obtain the fermented feed.
8. The preparation method of the fermented feed for prawns according to claim 7, wherein the fermentation temperature in the step (1) is 25-35 ℃; the fermentation time in the step (1) is 12-48 hours.
9. The preparation method of the fermented feed for prawns according to claim 7, wherein the particle size of the granules in the step (3) is 1.2mm, and the length of the granules is 1.8 mm.
10. The application of the fermented feed for the prawns is characterized in that the fermented feed is directly fed to the prawns.
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CN108949615A (en) * | 2018-06-25 | 2018-12-07 | 青岛农业大学 | Probiotics for the apostichopus japonicus Selenka feed that ferments |
CN109673816A (en) * | 2019-01-18 | 2019-04-26 | 郑州绿赛农牧业有限公司 | A kind of high-activity biological fermented feed used for aquiculture and preparation method thereof |
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CN102318778A (en) * | 2011-10-18 | 2012-01-18 | 上海创博生态工程有限公司 | Special feed for penaeus vannamei breeding and preparation method thereof |
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