CN110591900B - Cell recovery device - Google Patents

Cell recovery device Download PDF

Info

Publication number
CN110591900B
CN110591900B CN201910867559.7A CN201910867559A CN110591900B CN 110591900 B CN110591900 B CN 110591900B CN 201910867559 A CN201910867559 A CN 201910867559A CN 110591900 B CN110591900 B CN 110591900B
Authority
CN
China
Prior art keywords
fixedly connected
rack
test tube
shaft
connecting rod
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201910867559.7A
Other languages
Chinese (zh)
Other versions
CN110591900A (en
Inventor
不公告发明人
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shandong Quanxi Biotechnology Co ltd
Original Assignee
Shandong Quanxi Biotechnology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shandong Quanxi Biotechnology Co ltd filed Critical Shandong Quanxi Biotechnology Co ltd
Priority to CN201910867559.7A priority Critical patent/CN110591900B/en
Publication of CN110591900A publication Critical patent/CN110591900A/en
Application granted granted Critical
Publication of CN110591900B publication Critical patent/CN110591900B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00Means for pre-treatment of biological substances
    • C12M45/20Heating; Cooling
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00Means for pre-treatment of biological substances
    • C12M45/02Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting

Abstract

The invention relates to the field of cells, in particular to a cell recovery device, which can put a plurality of test tubes filled with cell solution into warm water, can swing in the front-back direction and the left-right direction in the water, can swing in a reciprocating way by taking the axis of a main shaft as a shaft, accelerates the recovery of cells in the warm water, and can fully mix the cells in a culture solution. The resuscitation pond comprises a pond body, support plates and sliding chutes, the pond body is in a rectangular box shape with an open upper end, the support plates are fixedly connected to the left end and the right end of the upper end of the pond body, and the sliding chutes are formed in the two support plates; rectangular block in the middle-end fixedly connected with of pivot, the equal fixedly connected with bead in both ends around the rectangular block of well, the left end fixedly connected with motor cabinet of pivot, the upper end fixedly connected with motor II of motor cabinet, fixedly connected with carousel II on motor II's the front end output shaft, carousel II's the articulated connecting rod II that is connected with in eccentric position.

Description

Cell recovery device
Technical Field
The invention relates to the field of cells, in particular to a cell recovery device.
Background
The invention discloses a cell resuscitator with the application number of CN201510694871.2, which comprises a water bath kettle and further comprises: the lifting mechanism and the bearing device are arranged on the lifting mechanism; and the controller is electrically connected with the lifting mechanism and used for controlling the movement of the lifting mechanism and putting the bearing device into the water bath kettle. The invention realizes the automatic mass cell recovery process by combining the lifting mechanism and the controller, can accurately control the recovery time and temperature, records the recovery process parameters, avoids the possible pollution of water in the water bath on the cryopreserved cells, greatly improves the cell recovery quality, lightens the burden of operators and improves the working efficiency. However, the cell resuscitator cannot shake cells in a bath, and cannot achieve the effects of rapid resuscitation and sufficient mixing of the cells in the culture solution.
Disclosure of Invention
The invention provides a cell recovery device, which has the advantages that a plurality of test tubes filled with cell solution can be placed in warm water, the test tubes can swing in the front-back direction and the left-right direction in the water, the plurality of test tubes filled with cell solution can swing in a reciprocating mode by taking the axis of a main shaft as a shaft, the recovery of cells in the warm water is accelerated, and the cells can be fully mixed in a culture solution.
The invention relates to the field of cells, in particular to a cell recovery device which comprises a recovery pool, a lifting frame, a swinging frame and a test tube rack.
The resuscitation pond comprises a pond body, support plates and sliding chutes, the pond body is in a rectangular box shape with an open upper end, the support plates are fixedly connected to the left end and the right end of the upper end of the pond body, and the sliding chutes are formed in the two support plates;
the swing frame comprises a rotating shaft, a rotary table II, a motor base, a connecting rod II, a middle rectangular block, ribs, connecting columns and a rack II, the middle end of the rotating shaft is fixedly connected with the middle rectangular block, the front end and the rear end of the middle rectangular block are fixedly connected with the ribs, the left end of the rotating shaft is fixedly connected with the motor base, the upper end of the motor base is fixedly connected with the motor II, the front end output shaft of the motor II is fixedly connected with the rotary table II, the eccentric position of the rotary table II is hinged with the connecting rod II, the right end of the rotating shaft is fixedly connected with the connecting column, the rack II is fixedly connected onto the connecting column, and the left end and the right end of the rotating shaft are respectively connected onto two sliding chutes in a sliding manner;
the test-tube rack includes the diaphragm, gear II, gear III, front and back flitch, articulated footstock, L shape is board even, main shaft and minor axis, flitch around the equal fixedly connected with in both ends around the board upper end is even to L shape, flitch sliding connection is on two beads respectively around two, fixedly connected with articulated footstock between the upper end of flitch around two, connecting rod II's the other end articulates on articulated footstock, L shape is even the lower extreme fixedly connected with diaphragm of board, the upper end of main shaft is passed through the bearing frame and is rotated the connection on the diaphragm, the upper end fixedly connected with gear III of main shaft, the minor axis passes through the bearing frame and rotates the connection on the diaphragm, the upper end fixedly connected with gear II of minor axis, gear II and gear III meshing transmission, gear II's rear end and rack II meshing transmission, the lower extreme of main shaft is provided with the test tube placer.
The resuscitation pond still includes the landing leg, and the landing leg is provided with four, and four landing legs are fixed connection respectively in four angle departments of the lower extreme of cell body, and the lower extreme of cell body is provided with the heating wire.
The resuscitation pond still includes fastening screw and fixed frame, the rear end fixedly connected with fixed frame of cell body, has fastening screw through threaded connection on the fixed frame.
The rocking frame further comprises a gear I, the left end and the right end of the rotating shaft are fixedly connected with the gear I, and the inner sides of the two gears I are attached to the outer sides of the two support plates.
The cell resuscitation device still includes the crane, the crane includes perpendicular traveller, rack I, the whippletree, the rack connecting rod, motor I, carousel I and connecting rod I, erect traveller sliding connection on fixed frame, fastening screw pushes up on erecting the traveller, the upper end fixedly connected with whippletree crossbar, both ends all sliding connection has rack I about the whippletree, fixedly connected with rack connecting rod between two rack I's the rear end, the upper end fixedly connected with motor I of whippletree, motor I's fixedly connected with carousel I on having the section output shaft, connecting rod I's one end articulates the eccentric position at carousel I, connecting rod I's the other end articulates the upper end at rack connecting rod, two rack I mesh the transmission with two gear I's lower extreme respectively.
The test-tube rack still includes board between disc, ring, test tube jack and connection, and the lower extreme fixed connection of main shaft is at the upper end center of disc, is the annular equipartition on the disc and has a plurality of test tube jacks, and the upper end fixed connection of board is on the disc between the connection, and the lower extreme fixed connection of board is on the ring between the connection, and the ring is located the lower extreme of a plurality of test tube jacks.
The main shaft, the disc and the circular ring are coaxial.
The cell recovery device has the beneficial effects that:
the invention provides a cell recovery device, which can put a plurality of test tubes filled with cell solution into warm water, can swing in the water in the front-back direction and the left-right direction, can swing in a reciprocating manner by taking the axis of a main shaft as an axis, accelerates the recovery of cells in the warm water, and can fully mix the cells in a culture solution.
Drawings
The invention is described in further detail below with reference to the accompanying drawings and specific embodiments.
FIG. 1 is a schematic diagram of the overall structure of a cell resuscitating device according to the present invention;
FIG. 2 is a schematic diagram of the overall structure of a cell resuscitating device of the present invention;
FIG. 3 is a first schematic structural diagram of a resuscitation pool;
FIG. 4 is a schematic structural diagram II of a resuscitation pool;
FIG. 5 is a first structural schematic diagram of the lifting frame;
FIG. 6 is a structural schematic diagram II of the lifting frame;
FIG. 7 is a first schematic structural view of the rocking beam;
FIG. 8 is a second schematic view of the structure of the rocking frame;
FIG. 9 is a first schematic structural view of a test tube rack;
fig. 10 is a structural schematic diagram of a test tube rack.
In the figure: a resuscitation pond 1; 1-1 parts of a tank body; 1-2 of supporting legs; 1-3 of a support plate; 1-4 of a chute; fastening screws 1-5; a fixed frame 1-6; a lifting frame 2; 2-1 of vertical sliding column; a rack I2-2; 2-3 of a horizontal bar; 2-4 of a rack connecting rod; 2-5 of a motor I; 2-6 of a turntable I; a connecting rod I2-7; a swing frame 3; gear I3-1; a rotating shaft 3-2; 3-3 of a turntable II; 3-4 of a motor II; 3-5 of a motor base; connecting rods II3-6; 3-7 parts of a middle rectangular block; 3-8 parts of convex ribs; connecting columns 3-9; 3-10 of a rack II; a test tube rack 4; a transverse plate 4-1; gear II4-2; gear III4-3; 4-4 of front and rear flitch plates; 4-5 of a hinged top seat; 4-6 of an L-shaped connecting plate; 4-7 of a main shaft; 4-8 of a disc; 4-9 parts of a circular ring; 4-10 parts of a test tube jack; the short shaft is 4-11; the intermediate plates 4-12 are connected.
Detailed Description
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, and are used only for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.
The first embodiment is as follows:
the present invention relates to the field of cells, and more particularly to a cell resuscitating device, which comprises a resuscitating pool 1, a lifting frame 2, a swinging frame 3 and a test tube rack 4, wherein a plurality of test tubes filled with cell solution can be placed in warm water, the test tubes can swing in the water in the front-back direction and the left-right direction, and the test tubes filled with cell solution can swing back and forth with the axis of a main shaft 4-7 as an axis, so that the resuscitating of cells in the warm water is accelerated, and the cells can be fully mixed in a culture solution.
The resuscitation pond 1 comprises a pond body 1-1, support plates 1-3 and sliding chutes 1-4, wherein the pond body 1-1 is in a rectangular box shape with an open upper end, the left end and the right end of the upper end of the pond body 1-1 are fixedly connected with the support plates 1-3, and the sliding chutes 1-4 are arranged on the two support plates 1-3; the tank body 1-1 is used for putting warm water so as to recover frozen cells.
The swing frame 3 comprises a rotating shaft 3-2, a rotary disc II3-3, a motor II3-4, a motor base 3-5, a connecting rod II3-6, a middle rectangular block 3-7, convex ribs 3-8, connecting columns 3-9 and racks II3-10, wherein the middle end of the rotating shaft 3-2 is fixedly connected with the middle rectangular block 3-7, the front end and the rear end of the middle rectangular block 3-7 are fixedly connected with the convex ribs 3-8, the left end of the rotating shaft 3-2 is fixedly connected with the motor base 3-5, the upper end of the motor base 3-5 is fixedly connected with the motor II3-4, the front end output shaft of the motor II3-4 is fixedly connected with the rotary disc II3-3, the eccentric position of the rotary disc II3-3 is hinged with the connecting rod II3-6, the right end of the rotating shaft 3-2 is fixedly connected with the connecting columns 3-9, the racks II3-10 are fixedly connected with the connecting columns 3-9, and the left and right ends of the rotating shaft 3-2 are respectively connected with the two sliding chutes 1-4 in a sliding manner; the left end and the right end of the rotating shaft 3-2 can rotate on the two sliding grooves 1-4 by self axes respectively, the left end and the right end of the rotating shaft 3-2 can also vertically slide on the two sliding grooves 1-4 respectively, and the output shaft of the motor II3-4 can drive the rotating disc II3-3 to rotate by taking the axis of the output shaft of the motor II3-4 as an axis when rotating.
The test tube rack 4 comprises a transverse plate 4-1, a gear II4-2, a gear III4-3, a front pasting plate 4-4, a rear pasting plate 4-4, a hinged top seat 4-5, an L-shaped connecting plate 4-6, a main shaft 4-7 and a short shaft 4-11, wherein the front end and the rear end of the upper end of the L-shaped connecting plate 4-6 are fixedly connected with the front pasting plate 4-4 and the rear pasting plate 4-4 respectively and slidably connected to two convex edges 3-8, the upper ends of the two front pasting plates 4-4 are fixedly connected with the hinged top seat 4-5, the other end of a connecting rod II3-6 is hinged to the hinged top seat 4-5, the lower end of the L-shaped connecting plate 4-6 is fixedly connected with a transverse plate 4-1, the upper end of the main shaft 4-7 is rotatably connected to the transverse plate 4-1 through a bearing seat, the upper end of the main shaft 4-7 is fixedly connected with the gear III4-3, the short shaft 4-11 is rotatably connected to the transverse plate 4-1 through the bearing seat, the upper end of the short shaft 4-11 is fixedly connected with the gear II4-2, the gear II4-2 is meshed with the gear III-3, and the lower end of the main shaft II-7 is provided with a test tube holder. The two front and rear pasting plates 4-4 can respectively slide on the two convex edges 3-8 left and right, the rotary table II3-3 can drive the hinged footstock 4-5 and the two front and rear pasting plates 4-4 to reciprocate left and right when rotating by taking the axis of the output shaft of the motor II3-4 as an axis, so that the whole test tube rack 4 can reciprocate left and right, the gear II4-2 and the short shaft 4-11 can rotate by taking the axis of the short shaft 4-11 as an axis when the transverse plate 4-1 reciprocates left and right, and the gear II4-2 drives the gear III4-3, the main shaft 4-7 and the test tube placer to reciprocate forward and backward by taking the axis of the main shaft 4-7 as an axis; the rotating shaft 3-2 rotates forwards and backwards by taking the axis of the rotating shaft as the shaft to drive the whole test tube rack 4 to swing back and forth, the test tube placer is used for placing a plurality of test tubes, and the plurality of test tubes can swing back and forth, swing left and right and rotate forwards and reversely by taking the axis of the main shaft 4-7 as the shaft. The recovery of the cells in warm water is accelerated, and the thawed cells can be fully mixed in the culture solution in the test tube.
The second embodiment is as follows:
the embodiment is described below with reference to fig. 1 to 10, and the embodiment further describes the first embodiment, the resuscitation pond 1 further includes four support legs 1-2, the four support legs 1-2 are respectively fixedly connected to four corners of the lower end of the pond body 1-1, and the lower end of the pond body 1-1 is provided with a heating wire. The supporting legs 1-2 play a supporting role, and the electric heating wires play a role in heating warm water in the tank body 1-1.
The third concrete implementation mode:
the embodiment is described below with reference to fig. 1 to 10, and the embodiment further describes the first embodiment, the resuscitation pool 1 further comprises fastening screws 1 to 5 and a fixing frame 1 to 6, the fixing frame 1 to 6 is fixedly connected to the rear end of the pool body 1 to 1, and the fastening screws 1 to 5 are connected to the fixing frame 1 to 6 through threads.
The fourth concrete implementation mode is as follows:
the third embodiment is further described with reference to fig. 1-10, the swing frame 3 further includes a gear I3-1, the left and right ends of the rotating shaft 3-2 are fixedly connected with the gear I3-1, and the inner sides of the two gears I3-1 are attached to the outer sides of the two support plates 1-3. The inner sides of the two gears I3-1 are attached to the outer sides of the two support plates 1-3, so that the rotating shaft 3-2 cannot move left and right between the two support plates 1-3.
The fifth concrete implementation mode:
the cell resuscitating device further comprises a lifting frame 2, wherein the lifting frame 2 comprises a vertical sliding column 2-1, a rack I2-2, a cross bar 2-3, a rack connecting rod 2-4, a motor I2-5, a rotary disc I2-6 and a connecting rod I2-7, the vertical sliding column 2-1 is connected to a fixing frame 1-6 in a sliding mode, a fastening screw 1-5 abuts against the vertical sliding column 2-1, the upper end of the vertical sliding column 2-1 is fixedly connected with the cross bar 2-3, the left end and the right end of the cross bar 2-3 are both connected with the rack I2-2 in a sliding mode, a rack connecting rod 2-4 is fixedly connected between the rear ends of the two racks I2-2, the upper end of the cross bar 2-3 is fixedly connected with the motor I2-5, a rotary disc I2-6 is fixedly connected to a section output shaft of the motor I2-5, one end of the connecting rod I2-7 is hinged to the eccentric position of the rotary disc I2-6, the other end of the connecting rod I2-7 is hinged to the upper end of the rack connecting rod 2-4, and the upper end of the rack I2-3 is respectively meshed with the lower end of a gear I2-3. The vertical sliding column 2-1 can vertically slide on the fixed frame 1-6, and the position of the vertical sliding column 2-1 can be fixed by rotating the fastening screw 1-5 to prop against the vertical sliding column 2-1. When the vertical sliding column 2-1 rises or falls, the horizontal bar 2-3 and the two racks I2-2 are driven to rise or fall, the two racks I2-2 respectively drive the two gears I3-1 and the whole swing frame 3 to rise or fall, so that the test tube rack 4 rises or falls along with the swing frame 3, and the depth of the test tubes immersed in warm water is adjusted according to the sizes of the test tubes. When the output shaft of the motor I2-5 rotates, the rotary table I2-6 is driven to rotate by taking the axis of the output shaft of the motor I2-5 as a shaft, the rotary table I2-6 drives the rack connecting rod 2-4 to reciprocate back and forth through the connecting rod I2-7, the rack connecting rod 2-4 drives the two racks I2-2 to reciprocate back and forth, and the two racks I2-2 respectively drive the two gears I3-1 to reciprocate back and forth when reciprocating back and forth, so that the rotary shaft 3-2 is driven to rotate back and forth by taking the axis of the rotary table I2-5 as a shaft.
The sixth specific implementation mode:
the following describes the present embodiment with reference to fig. 1 to 10, which further describes the first embodiment, the test tube rack 4 further includes a disk 4 to 8, a ring 4 to 9, test tube jacks 4 to 10, and a connection intermediate plate 4 to 12, the lower end of the main shaft 4 to 7 is fixedly connected to the center of the upper end of the disk 4 to 8, a plurality of test tube jacks 4 to 10 are annularly and uniformly distributed on the disk 4 to 8, the upper end of the connection intermediate plate 4 to 12 is fixedly connected to the disk 4 to 8, the lower end of the connection intermediate plate 4 to 12 is fixedly connected to the ring 4 to 9, and the ring 4 to 9 is located at the lower end of the plurality of test tube jacks 4 to 10. The test tube inserting holes 4-10 are used for inserting test tubes, the bottoms of the test tubes are supported on the circular rings 4-9, and the test tubes cannot slide off the inserting holes 4-10.
The seventh embodiment:
the present embodiment is described below with reference to fig. 1 to 10, and the present embodiment further describes the first embodiment, in which the main shafts 4 to 7, the disks 4 to 8, and the rings 4 to 9 are coaxial.
The working principle of the invention is as follows: the tank body 1-1 is used for putting warm water so as to recover frozen cells. The left end and the right end of the rotating shaft 3-2 can rotate on the two sliding grooves 1-4 by self axes respectively, the left end and the right end of the rotating shaft 3-2 can also vertically slide on the two sliding grooves 1-4 respectively, and the output shaft of the motor II3-4 can drive the rotating disc II3-3 to rotate by taking the axis of the output shaft of the motor II3-4 as an axis when rotating. The two front and rear pasting plates 4-4 can respectively slide left and right on the two convex edges 3-8, the rotary table II3-3 can drive the hinged footstock 4-5 and the two front and rear pasting plates 4-4 to reciprocate left and right when rotating by taking the axis of the output shaft of the motor II3-4 as an axis, so that the whole test tube rack 4 can reciprocate left and right, when the transverse plate 4-1 reciprocates left and right, the gear II4-2 and the short shaft 4-11 can both rotate by taking the axis of the short shaft 4-11 as an axis, and the gear II4-2 drives the gear III4-3, the main shaft 4-7 and the test tube placer to reciprocate forward and backward by taking the axis of the main shaft 4-7 as an axis; the rotating shaft 3-2 can drive the whole test tube rack 4 to swing back and forth by taking the axis of the rotating shaft as the axis and rotate back and forth, the test tube placer is used for placing a plurality of test tubes, and the plurality of test tubes can swing back and forth, swing left and right and rotate forward and backward by taking the axis of the main shaft 4-7 as the axis. The recovery of the cells in warm water is accelerated, and the thawed cells can be fully mixed in a culture solution in a test tube. The supporting legs 1-2 play a supporting role, and the electric heating wires play a role in heating warm water in the tank body 1-1. The inner sides of the two gears I3-1 are attached to the outer sides of the two support plates 1-3, so that the rotating shaft 3-2 cannot move left and right between the two support plates 1-3. The vertical sliding column 2-1 can vertically slide on the fixed frame 1-6, and the position of the vertical sliding column 2-1 can be fixed by rotating the fastening screw 1-5 to prop against the vertical sliding column 2-1. When the vertical sliding column 2-1 rises or falls, the horizontal bar 2-3 and the two racks I2-2 are driven to rise or fall, the two racks I2-2 respectively drive the two gears I3-1 and the whole swing frame 3 to rise or fall, so that the test tube rack 4 rises or falls along with the swing frame 3, and the depth of the test tubes immersed in warm water is adjusted according to the sizes of the test tubes. When the output shaft of the motor I2-5 rotates, the rotary table I2-6 is driven to rotate by taking the axis of the output shaft of the motor I2-5 as a shaft, the rotary table I2-6 drives the rack connecting rod 2-4 to reciprocate back and forth through the connecting rod I2-7, the rack connecting rod 2-4 drives the two racks I2-2 to reciprocate back and forth, and the two racks I2-2 respectively drive the two gears I3-1 to reciprocate back and forth when reciprocating back and forth, so that the rotary shaft 3-2 is driven to rotate back and forth by taking the axis of the rotary table I2-5 as a shaft. The test tube inserting holes 4-10 are used for inserting test tubes, the bottoms of the test tubes are supported on the circular rings 4-9, and the test tubes cannot slide off the inserting holes 4-10.
It is to be understood that the above description is not intended to limit the present invention, and the present invention is not limited to the above examples, and that various changes, modifications, additions and substitutions which are within the spirit and scope of the present invention and which may be made by those skilled in the art are also within the scope of the present invention.

Claims (2)

1. The utility model provides a cell recovery device, includes recovery pond (1), rocking arm (3) and test-tube rack (4), its characterized in that: the resuscitation pond (1) comprises a pond body (1-1), support plates (1-3) and sliding chutes (1-4), wherein the pond body (1-1) is in a rectangular box shape with an open upper end, the support plates (1-3) are fixedly connected to the left end and the right end of the upper end of the pond body (1-1), and the sliding chutes (1-4) are arranged on the two support plates (1-3);
the swing frame (3) comprises a rotating shaft (3-2), a rotary table II (3-3), a motor II (3-4), a motor base (3-5), a connecting rod II (3-6), a middle rectangular block (3-7), ribs (3-8), connecting columns (3-9) and racks II (3-10), the middle end of the rotating shaft (3-2) is fixedly connected with the middle rectangular block (3-7), the front end and the rear end of the middle rectangular block (3-7) are fixedly connected with the ribs (3-8), the left end of the rotating shaft (3-2) is fixedly connected with the motor base (3-5), the upper end of the motor base (3-5) is fixedly connected with the motor II (3-4), the front end output shaft of the motor II (3-4) is fixedly connected with the rotary table II (3-3), the eccentric position of the rotary table II (3-3) is hinged with the connecting rod II (3-6), the right end of the rotating shaft (3-2) is fixedly connected with the connecting columns (3-9), the racks II (3-10) is fixedly connected with the connecting columns (3-9), and the left and right ends of the connecting columns (3-2) are respectively connected with two chutes (1-4) in a sliding way;
the test tube rack (4) comprises a transverse plate (4-1), a gear II (4-2), a gear III (4-3), front and rear flitch plates (4-4), a hinged footstock (4-5), an L-shaped connecting plate (4-6), a main shaft (4-7) and a short shaft (4-11), wherein the front end and the rear end of the upper end of the L-shaped connecting plate (4-6) are fixedly connected with the front and rear flitch plates (4-4), the two front and rear flitch plates (4-4) are respectively connected on two convex edges (3-8) in a sliding manner, the hinged footstock (4-5) is fixedly connected between the upper ends of the two front and rear flitch plates (4-4), the other end of a connecting rod II (3-6) is hinged on the hinged footstock (4-5), the lower end of the L-shaped connecting plate (4-6) is fixedly connected with the transverse plate (4-1), the upper end of the main shaft (4-7) is rotatably connected on the transverse plate (4-1) through a bearing seat, the upper end of the main shaft (4-7) is fixedly connected with the gear III (4-3), the short shaft (4-11) is rotatably connected on the transverse plate (4-1), the gear III) and the gear (4-2) is meshed with the gear (4-3), the rear end of the gear II (4-2) is in meshing transmission with the rack II (3-10), and the lower end of the main shaft (4-7) is provided with a test tube placer;
the resuscitation pond (1) further comprises four support legs (1-2), the four support legs (1-2) are respectively fixedly connected to four corners of the lower end of the pond body (1-1), and the lower end of the pond body (1-1) is provided with an electric heating wire;
the swing frame (3) further comprises gears I (3-1), the left end and the right end of the rotating shaft (3-2) are fixedly connected with the gears I (3-1), and the inner sides of the two gears I (3-1) are attached to the outer sides of the two support plates (1-3);
the cell recovery device also comprises a lifting frame (2), the lifting frame (2) comprises a vertical sliding column (2-1), a rack I (2-2) and a horizontal bar (2-3), the rack-and-pinion type sliding frame comprises rack connecting rods (2-4), motors I (2-5), rotating discs I (2-6) and connecting rods I (2-7), wherein a vertical sliding column (2-1) is connected to a fixed frame (1-6) in a sliding mode, fastening screws (1-5) are supported against the vertical sliding column (2-1), the upper end of the vertical sliding column (2-1) is fixedly connected with a cross bar (2-3), the left end and the right end of the cross bar (2-3) are connected with racks I (2-2) in a sliding mode, a rack connecting rod (2-4) is fixedly connected between the rear ends of the two racks I (2-2), the upper end of the cross bar (2-3) is fixedly connected with the motors I (2-5), the rotating discs I (2-6) are fixedly connected to section output shafts of the motors I (2-5), one end of each connecting rod I (2-7) is hinged to the eccentric position of the corresponding rotating disc I (2-6), the other end of each rack I (2-7) is hinged to the upper end of the corresponding connecting rod (2-4), and the two racks I (2-2) are respectively meshed with the lower ends of the corresponding gears (3-1-3);
the test tube placer is characterized in that the test tube rack (4) further comprises a disc (4-8), a ring (4-9), test tube jacks (4-10) and connecting intermediate plates (4-12), the lower end of a main shaft (4-7) is fixedly connected to the center of the upper end of the disc (4-8), a plurality of test tube jacks (4-10) are uniformly distributed on the disc (4-8) in an annular mode, the upper end of the connecting intermediate plate (4-12) is fixedly connected to the disc (4-8), the lower end of the connecting intermediate plate (4-12) is fixedly connected to the ring (4-9), the ring (4-9) is located at the lower ends of the test tube jacks (4-10), and the disc (4-8), the ring (4-9), the test tube jacks (4-10) and the connecting intermediate plates (4-12) form the test tube placer.
2. A cell resuscitation device according to claim 1, wherein: the main shaft (4-7), the disc (4-8) and the circular ring (4-9) are coaxial.
CN201910867559.7A 2018-11-22 2018-11-22 Cell recovery device Active CN110591900B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910867559.7A CN110591900B (en) 2018-11-22 2018-11-22 Cell recovery device

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201910867559.7A CN110591900B (en) 2018-11-22 2018-11-22 Cell recovery device
CN201811399432.9A CN109401917B (en) 2018-11-22 2018-11-22 A kind of cell recovery device

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201811399432.9A Division CN109401917B (en) 2018-11-22 2018-11-22 A kind of cell recovery device

Publications (2)

Publication Number Publication Date
CN110591900A CN110591900A (en) 2019-12-20
CN110591900B true CN110591900B (en) 2022-10-25

Family

ID=65474439

Family Applications (3)

Application Number Title Priority Date Filing Date
CN201910867559.7A Active CN110591900B (en) 2018-11-22 2018-11-22 Cell recovery device
CN201811399432.9A Active CN109401917B (en) 2018-11-22 2018-11-22 A kind of cell recovery device
CN201910867560.XA Active CN110724624B (en) 2018-11-22 2018-11-22 Cell recovery device

Family Applications After (2)

Application Number Title Priority Date Filing Date
CN201811399432.9A Active CN109401917B (en) 2018-11-22 2018-11-22 A kind of cell recovery device
CN201910867560.XA Active CN110724624B (en) 2018-11-22 2018-11-22 Cell recovery device

Country Status (1)

Country Link
CN (3) CN110591900B (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110157599A (en) * 2019-07-10 2019-08-23 陕西中医药大学 A kind of culture device of pilot scale culture umbilical cord mesenchymal stem cells
CN112899129A (en) * 2021-01-15 2021-06-04 吴应迪 Cell resuscitator with lifting function
CN112961775B (en) * 2021-03-10 2023-05-12 秦娟 Cell resuscitator
CN113073033A (en) * 2021-03-23 2021-07-06 曾小广 Cell rapid recovery device for medical clinical laboratory
CN113999771B (en) * 2021-12-31 2022-04-08 迈赛凯尔(山东)生命科学有限公司 Immature dendritic cell recovery device
CN116555029A (en) * 2023-05-16 2023-08-08 宁波宇泰医疗器械有限公司 Stem cell culture system with high survival rate

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2840459A (en) * 1949-05-04 1958-06-24 Blaw Knox Co Continuous solvent extractor and solvent extraction system
GB767269A (en) * 1953-08-14 1957-01-30 Lewis Berger & Sons Ltd Improvements in or relating to mixing machines for dispensing liquids
US3432268A (en) * 1964-08-28 1969-03-11 Peter Unger Method and apparatus for testing cell suspensions
WO2001038882A1 (en) * 1999-11-23 2001-05-31 Becton Dickinson And Company Apparatus and method for processing sample materials contained in a plurality of sample tubes
US10555374B2 (en) * 2014-05-16 2020-02-04 Biolife Solutions, Inc. Systems, devices, and methods for automated sample thawing
CN204779570U (en) * 2015-06-01 2015-11-18 沈阳澔源生物科技有限公司 Electronic cell resuscitator
CN105199950B (en) * 2015-10-22 2018-01-16 上海原能细胞医学技术有限公司 Cell resuscitator
WO2018021359A1 (en) * 2016-07-25 2018-02-01 宇部興産株式会社 Siphon type cultivation method
CN206082616U (en) * 2016-10-10 2017-04-12 夏全兵 Division of endocrinology uses test -tube rack
CN206476980U (en) * 2016-12-27 2017-09-08 深圳市第二人民医院 A kind of automatic recovery instrument of cell
CN206396248U (en) * 2017-01-16 2017-08-11 深圳市第二人民医院 A kind of auxiliary cell culture apparatus
CN107189931A (en) * 2017-06-26 2017-09-22 广州瑞能医学科技有限公司 A kind of cell collector
CN206897464U (en) * 2017-06-26 2018-01-19 广州瑞能医学科技有限公司 A kind of clamping device
CN207062260U (en) * 2017-08-03 2018-03-02 郑州伊美诺生物技术有限公司 Cell recovery thawing water-bath
CN107475189B (en) * 2017-10-12 2020-12-22 北京唐颐惠康生物医学技术有限公司 Dental pulp stem cell collecting set
CN109777735A (en) * 2018-03-26 2019-05-21 安徽鹰龙工业设计有限公司 A kind of cell engineering various dimensions cell rocking equipment

Also Published As

Publication number Publication date
CN110724624A (en) 2020-01-24
CN110724624B (en) 2022-11-11
CN109401917A (en) 2019-03-01
CN110591900A (en) 2019-12-20
CN109401917B (en) 2019-10-29

Similar Documents

Publication Publication Date Title
CN110591900B (en) Cell recovery device
CN209793750U (en) automatic noodle boiling equipment with multi-shaft mechanical arm
CN209941121U (en) Screw rod surface electroplating device
CN109401916B (en) Translation formula cell recovery device
CN107261956A (en) A kind of automated processing equipment of food processing field
CN210950554U (en) Adjustable projection device for training
CN112630101A (en) Cement mortar fluidity detection system capable of improving detection effect
CN108722283B (en) Mixing device is used in a kind of processing of lifting type new material
CN208967400U (en) A kind of unattended safety patrol inspection device in interior
CN207107926U (en) Lift material-strewing device
CN206915668U (en) A kind of full-automatic jacking locating reversing device
CN109730782A (en) A kind of electronic lifting tray of low cost
CN212758161U (en) Raise cattle and use fodder mixing machine
CN209276167U (en) A kind of piece of glue continous way glue aging integrated device
CN211159450U (en) Mechanical stirrer for metallurgy
CN206543566U (en) A kind of food processing of mechanical field raw material mixing apparatus
CN208163566U (en) A kind of maintenance platform for entertaining overhead falling sphere loop wheel machine
CN207137857U (en) A kind of paint mixer simple rack
CN112642960A (en) Steel bar straightening and cutting device for construction machinery
CN206963188U (en) A kind of service sink mechanism of paster rectifier bridge solvent-borne type circuit board cleaning machine
CN111229151A (en) Polycarboxylate bulky concrete high efficiency slow setting water reducing agent preparation system
CN217189161U (en) Movable agitating unit for hair conditioner
CN219877855U (en) Rack is used in mechanical equipment sales
CN213966157U (en) Powder agitator tank motor device
CN209515895U (en) Lithium ion battery formation device

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
DD01 Delivery of document by public notice
DD01 Delivery of document by public notice

Addressee: Shaping treasure

Document name: Business Review letter

TA01 Transfer of patent application right
TA01 Transfer of patent application right

Effective date of registration: 20221010

Address after: Room 01, Building 01, Block 5, Yinfeng Biological City, No. 1177, Chunlan Road, High tech Zone, Jinan, Shandong 250000

Applicant after: Shandong Quanxi Biotechnology Co.,Ltd.

Address before: 213000 Jinling North Road, Xinbei District, Changzhou City, Jiangsu Province, No. 20

Applicant before: Chen Shuangshuang

GR01 Patent grant
GR01 Patent grant