CN207062260U - Cell recovery thawing water-bath - Google Patents
Cell recovery thawing water-bath Download PDFInfo
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- CN207062260U CN207062260U CN201720960543.7U CN201720960543U CN207062260U CN 207062260 U CN207062260 U CN 207062260U CN 201720960543 U CN201720960543 U CN 201720960543U CN 207062260 U CN207062260 U CN 207062260U
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- bath
- water
- tank
- drive rod
- tube sheet
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Abstract
The utility model discloses a kind of cell recovery thawing water-bath, including water-bath casing, tank and control cabinet are provided with water-bath casing, bottom of gullet sets having heaters, its top movable is provided with pot cover, the edge of sink top is higher than water-bath casing, a pair of slide rails are symmetrically arranged with sink top edge, across being provided with drive rod on slide rail, tube sheet is centrifuged across the U-shaped being provided with along slide rail back and forth movement on drive rod both ends or sliding part, uniform intervals offer the through hole for placing centrifuge tube on the bottom plate of U-shaped centrifugation tube sheet.The utility model advantage is to promote centrifugation tube sheet to move back and forth using electric pushrod, reduces workload, effectively ensure that cell line all melted in one minute, improve operating efficiency, ensure the survival rate of cell；Pot cover can be covered on during thawing on tank, and experimenter can keep certain distance with water-bath, effectively reduce the probability of centrifuge tube booster damage experiment personnel, it is ensured that laboratory technician's safety.
Description
Technical field
Laboratory apparatus is the utility model is related to, more particularly, to a kind of cell recovery thawing water-bath.
Background technology
In monoclonal antibody preparation, generally require to deposit cell suspension point in the centrifuge tube for being 1.5mL to volume, then
Centrifuge tube is freezed（Liquid nitrogen frozen）, to meet experimental arrangement.When carrying out subsequent experimental, it is necessary to the cell recovery that will be frozen；
During cell recovery, centrifuge tube is generally placed on speed in 35 DEG C or so of water-bath and melted, and need not stop manually when speed is melted
Rock, to ensure uniform fast melt.But there is problems with above-mentioned resuscitation process：To make cell line fast melt, generally
Need manually to rock centrifuge tube, not only increase workload, while easy damage experiment personnel after centrifuge tube booster；Because cell is answered
Soviet Union's amount is big（Individual centrifuge tube is even more more up to more than 100）, cell could be completed by least needing during practical operation two people to work one day
Resuscitative efforts, time-consuming, influences experiment progress；Dynamics inequality is rocked due to artificial, the cell recovery time is different, the long period
Recovery cause intracellular ice crystal to cause secondary injury to cell, influence subsequent experimental result.
The content of the invention
The utility model purpose is to provide a kind of thawing efficiency fast and time saving and energy saving cell recovery thawing water-bath.
To achieve the above object, the utility model takes following technical proposals：
Cell recovery thawing water-bath described in the utility model, including water-bath casing, on the water-bath casing
The control cabinet for being in the tank of rectangular parallelepiped structure and being electrically connected with external power supply is provided with, the bottom of gullet sets having heaters,
Its top movable is provided with pot cover, and the edge of the sink top is higher than the water-bath casing, before sink top edge
The slide rail of a pair of Longitudinal extendings is symmetrically arranged with afterwards, by sliding part across being provided with by power source drive on the slide rail
Drive rod, the drive rod are past along slide rail across being provided with drive rod both ends or sliding part across being arranged on above tank
The U-shaped centrifugation tube sheet of motion is returned, the bottom plate of the U-shaped centrifugation tube sheet is arranged in the tank, on the bottom plate of U-shaped centrifugation tube sheet
Uniform intervals offer the through hole for placing centrifuge tube, and the through hole is arranged in rows.
The power source is to be arranged on the electric pushrod at the top of the control cabinet, and the push rod of the electric pushrod passes through pin
It is connected with the axle sleeve in the middle part of the drive rod.
The sliding part includes a pair of sliding blocks, and the sliding block is in character "door" form structure.
The through hole is at least two rows.
The utility model advantage, which is to promote using electric pushrod, centrifuges tube sheet reciprocating motion, reduces workload, effectively really
Protect cell line in one minute all to melt, improve operating efficiency, and the cell line melting rate in each centrifuge tube is basically identical,
Ensure the survival rate of cell；Pot cover can be covered on during thawing on tank, and experimenter can keep certain distance with water-bath, have
Effect reduces the probability of centrifuge tube booster damage experiment personnel, it is ensured that laboratory technician's safety.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model.
Fig. 2 is Fig. 1 left view structural formula schematic diagram（Conceal electric pushrod）.
Embodiment
As shown in Figure 1 and Figure 2, cell recovery thawing water-bath described in the utility model, set on water-bath casing 1
There is the control cabinet 3 for being in the tank 2 of rectangular parallelepiped structure and being electrically connected with external power supply, the bottom of tank 2 sets having heaters 4, and it is pushed up
Portion is movably set with pot cover, and the top sides of tank 2 are symmetrically arranged with along water-bath casing 1 is higher than before and after the top edge of tank 2
The slide rail 5 of a pair of Longitudinal extendings, slide on slide rail 5 and be provided with sliding part, sliding part include be in for a pair character "door" form structure sliding blocks
12.1st, 12.2, two sliding blocks 12.1,12.2 are slidably arranged on slide rail 5 corresponding thereto respectively, two sliding blocks 12.1,12.2 it
Between across being fixed with the drive rod 7 that is driven by power source 6, drive rod 7 is located at the top of tank 2, and power source 6 is is arranged on control cabinet
The electric pushrod at 3 tops, the push rod 10 of electric pushrod are connected by pin with the axle sleeve 11 at the middle part of drive rod 7, electric pushrod
Push rod 10 and axle sleeve 11 it is detachable；Also it is located at U in tank 2 positioned at bottom plate across being fixed between two sliding blocks 12.1,12.2
Shape centrifuges tube sheet 8, and U-shaped centrifuges tube sheet 8 and moved back and forth by sliding block 12.1,12.2 along the length direction of slide rail 5, U-shaped centrifugation tube sheet 8
Bottom plate on uniform intervals offer the through hole 9 that centrifuge tube is put in two discharges, per exhausting hole 9 quantity be 10, make centrifuge tube bottom
Portion contacts with deionized water, it is ensured that melts effect, while can be required to determine cell line recovery quantity according to practical operation.
In use, add deionized water into tank 2（The depth of deionized water is with just by the cell ice cube in centrifuge tube
Flood and be preferred, it is ensured that centrifuge tube lid avoids polluting away from deionized water）, control cabinet 3 is electrically connected with external power source, passed through
The temperature of deionized water, is added by heater 4 to deionized water in temperature control panel setting tank 2 on control cabinet 3
Heat, by water temperature heating to 37 DEG C, it then will be stored in centrifuge tube in liquid nitrogen, equipped with cell line with large size tweezers and correspondingly put
Enter in through hole 9, place and complete pot cover while start electric pushrod, drive U-shaped to centrifuge tube sheet 8 by electric pushrod and come and go
Rock centrifuge tube（Jitter time is one minute）, cell line is melted completely, then take out cell line, carry out follow-up recovery step
Suddenly；After the completion for the treatment of this group of cell line recovery, aforesaid operations are repeated, carry out the recovery of next group of cell line.This water-bath has used
Cheng Hou, deionized water is released by the delivery port of the side of tank 2, in order to which next time uses.
The advantages of the utility model, is embodied in following aspect：
1st, it is time saving and energy saving, melt efficiency high, reduce workload：The back and forth movement of drive rod 7, driving are driven by electric pushrod
Bar 7 drives U-shaped to centrifuge tube sheet 8 along the back and forth movement of slide rail 5 by sliding block 12.1,12.2, rocks uniformly, improves and melt efficiency, together
When ensure cell survival rate.
2nd, it is safe：Pot cover can be covered on tank 2 during rocking, experimenter can be away from a spacing when rocking
From avoiding centrifuge tube booster damage experiment personnel, it is ensured that experiment safety.
Claims (4)
1. a kind of cell recovery thawing water-bath, including water-bath casing（1）, the water-bath casing（1）On be provided with and be in
The tank of rectangular parallelepiped structure（2）With the control cabinet electrically connected with external power supply（3）, the tank（2）Bottom sets having heaters
（4）, its top movable is provided with pot cover, it is characterised in that：The tank（2）The edge at top is higher than the water-bath casing
（1）, in tank（2）The slide rail of a pair of Longitudinal extendings is symmetrically arranged with before and after the edge of top（5）, the slide rail（5）On pass through
Sliding part is across being provided with by power source（6）The drive rod of driving（7）, the drive rod（7）Across being arranged on tank（2）On
Side, drive rod（7）Across being provided with along slide rail on both ends or sliding part（5）The U-shaped centrifugation tube sheet of back and forth movement（8）, the U
Shape centrifuges tube sheet（8）Bottom plate be arranged on the tank（2）It is interior, U-shaped centrifugation tube sheet（8）Bottom plate on uniform intervals offer and put
Put the through hole of centrifuge tube（9）, the through hole（9）It is arranged in rows.
2. cell recovery thawing water-bath according to claim 1, it is characterised in that：The power source（6）To set
In the control cabinet（3）The electric pushrod at top, the push rod of the electric pushrod（10）Pass through pin and the drive rod（7）In
The axle sleeve in portion（11）It is connected.
3. cell recovery thawing water-bath according to claim 1, it is characterised in that：The sliding part includes a pair of cunnings
Block, the sliding block are in character "door" form structure.
4. cell recovery thawing water-bath according to claim 1, it is characterised in that：The through hole（9）At least two
Row.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720960543.7U CN207062260U (en) | 2017-08-03 | 2017-08-03 | Cell recovery thawing water-bath |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720960543.7U CN207062260U (en) | 2017-08-03 | 2017-08-03 | Cell recovery thawing water-bath |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207062260U true CN207062260U (en) | 2018-03-02 |
Family
ID=61519369
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720960543.7U Active CN207062260U (en) | 2017-08-03 | 2017-08-03 | Cell recovery thawing water-bath |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207062260U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109401917A (en) * | 2018-11-22 | 2019-03-01 | 修宝利 | A kind of cell recovery device |
| CN109590046A (en) * | 2019-02-14 | 2019-04-09 | 杨柳 | A kind of smart sample recovery box of explosion-proof jelly |
| CN109652487A (en) * | 2019-01-21 | 2019-04-19 | 国药肽谷有限公司 | A kind of extracting method with the honey peptide for improving cardiovascular and cerebrovascular disease |
-
2017
- 2017-08-03 CN CN201720960543.7U patent/CN207062260U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109401917A (en) * | 2018-11-22 | 2019-03-01 | 修宝利 | A kind of cell recovery device |
| CN109401917B (en) * | 2018-11-22 | 2019-10-29 | 山东翰康生物科技有限公司 | A kind of cell recovery device |
| CN109652487A (en) * | 2019-01-21 | 2019-04-19 | 国药肽谷有限公司 | A kind of extracting method with the honey peptide for improving cardiovascular and cerebrovascular disease |
| CN109590046A (en) * | 2019-02-14 | 2019-04-09 | 杨柳 | A kind of smart sample recovery box of explosion-proof jelly |
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