CN107189931A - A kind of cell collector - Google Patents
A kind of cell collector Download PDFInfo
- Publication number
- CN107189931A CN107189931A CN201710492615.4A CN201710492615A CN107189931A CN 107189931 A CN107189931 A CN 107189931A CN 201710492615 A CN201710492615 A CN 201710492615A CN 107189931 A CN107189931 A CN 107189931A
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- rotating
- clamping device
- liquid
- test tube
- table apparatus
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- 238000012360 testing method Methods 0.000 claims abstract description 62
- 239000007788 liquid Substances 0.000 claims abstract description 59
- 238000003756 stirring Methods 0.000 claims abstract description 22
- 238000005086 pumping Methods 0.000 claims abstract description 13
- 238000001514 detection method Methods 0.000 claims description 10
- 238000005119 centrifugation Methods 0.000 claims description 9
- 230000005540 biological transmission Effects 0.000 claims description 6
- 230000003534 oscillatory effect Effects 0.000 claims description 5
- 238000013019 agitation Methods 0.000 claims description 3
- 238000007689 inspection Methods 0.000 claims 1
- 238000010276 construction Methods 0.000 abstract description 4
- 230000006641 stabilisation Effects 0.000 description 16
- 238000011105 stabilization Methods 0.000 description 16
- 230000007246 mechanism Effects 0.000 description 13
- 229920001971 elastomer Polymers 0.000 description 8
- 239000000806 elastomer Substances 0.000 description 8
- 230000000007 visual effect Effects 0.000 description 7
- 238000000034 method Methods 0.000 description 5
- 238000002156 mixing Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 description 3
- 238000000354 decomposition reaction Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000000703 high-speed centrifugation Methods 0.000 description 2
- 230000008520 organization Effects 0.000 description 2
- 230000010355 oscillation Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 238000005299 abrasion Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000005693 optoelectronics Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
Abstract
The present invention is intended to provide a kind of cell collector, including a frame and the self-balancing rotating disk being arranged in frame, the liquid-adding device of adding liquid into test tube, apparatus for combining liquids and the pumping device for extracting liquid in test tube, self-balancing rotating disk includes rotating-table apparatus and the rotating blade drive rotated for driving rotating disk device, apparatus for combining liquids includes some agitating devices for the clamping device of holding test tubes and for stirring clamping device, clamping device is arranged on rotating-table apparatus, agitating device is arranged on below clamping device, liquid-adding device and pumping device are arranged on above clamping device.The present invention is simple in construction, can it is automatic, efficiently, quickly complete cell collection, overcome artificially collect cell it is cumbersome, it is inefficient with it is error-prone etc. the problem of.
Description
Technical field
The present invention relates to a kind of cell collector.
Background technology
Cell collects the mission requirements for being scientific research and being frequently referred in experiment, and cell is collected general complete in test tube
Into, and cell collects the operation repeatedly for often relating to the processes such as liquid mixing, liquid centrifugal analysis.Presently, cell is received
What collection was often completed stage by stage, each stage is required for cell gatherer constantly to follow up and operate in person, and this is not only added
The workload of cell gatherer, and cell collection process is cumbersome, error-prone, inefficiency, it is impossible to scientific research is met well
With the demand of experiment, associated biomolecule and the demand of medical technology promotion and application can not be particularly met.
The content of the invention
To overcome the defect of above-mentioned prior art, the embodiments of the invention provide a kind of cell collector, its is simple in construction,
Energy is automatic, efficient, quickly complete cell collection.
In order to achieve the above object there is provided a kind of cell collector, including a frame and the self-balancing that is arranged in frame
Rotating disk, the pumping device toward liquid in the liquid-adding device of adding liquid, apparatus for combining liquids and extraction test tube in test tube are certainly flat
The rotating disk that weighs includes rotating-table apparatus and the rotating blade drive rotated for driving rotating disk device, and apparatus for combining liquids includes some use
In the clamping device of holding test tubes and agitating device for stirring clamping device, clamping device is arranged on rotating-table apparatus, stirred
Mix device to be arranged on below clamping device, liquid-adding device and pumping device are arranged on above clamping device.
It is preferred that, clamping device includes a mounting seat, a swing component and a fastener for carrying test tube, installs
Seat is arranged on rotating-table apparatus, and swing component is connected with mounting seat by the first swinging axle so that swing component can be mounted opposite seat pendulum
Dynamic, fastener is connected with swing component by the second swinging axle so that fastener can the swing of opposing oscillatory part, and fastener
It is connected with agitating device.
It is preferred that, the first swinging axle is in vertical distribution with the second swinging axle.
It is preferred that, an attachment means are provided between agitating device and clamping device, when need stir test tube in liquid
When, agitating device is connected with clamping device, and after the aqueous agitation in test tube is finished, agitating device is separated with clamping device, even
Connection device includes the pin end that a female joint being arranged on agitating device is arranged on one on clamping device.
It is preferred that, agitating device includes an eccentric wheel and the stirring motor rotated with movable eccentric wheel, and female joint sets inclined
On heart wheel so that stirring motor can drive female joint to do eccentric circular motion.
It is preferred that, a connection locator is provided with agitating device, when agitating device is connected with clamping device, connection
Positioner causes stirring motor to go to set angle.
It is preferred that, clamping device is symmetrically distributed on using the rotating shaft of rotating-table apparatus as the circumference in the center of circle.
It is preferred that, self-balancing rotating disk includes a balance detecting device, when balance detecting device is detected with rotating-table apparatus
Rotating shaft is symmetrical for the test tube on the circumference in the center of circle, and rotating blade drive drives rotating-table apparatus to do centrifugal motion.
It is preferred that, cell collector also includes liquid level detection device, and liquid level detection device is taken out to detect from test tube
Whether the liquid volume taken is consistent.
It is preferred that, rotating blade drive includes a centrifugation motor, transmission device and a gear unit, and centrifugation motor passes through
Transmission device drives gear unit to rotate, and gear unit is connected with rotating-table apparatus.
Present invention advantage compared with prior art is：
The present invention is simple in construction, can it is automatic, efficiently, quickly complete cell collection, overcome artificially collect cell it is cumbersome,
Inefficient and error-prone etc. the problem of.Meanwhile, the self-balancing rotating disk in the present invention takes into full account when structure design is with running
Rotating disk does the balance and stability during high speed centrifugation motion, and the effect of cell separation is ensure that well.In addition, in the present invention
Apparatus for combining liquids the swing that clamping device is driven by the centrifugal motion of agitating device is set, and clamping device is provided with the
One swinging axle and the second swinging axle, have fully ensured the mixing of the liquid in clamping device in test tube.
Brief description of the drawings
Fig. 1 is the schematic diagram at cell collector overall structure one visual angle；
Fig. 2 is cell collector overall structure secondly the schematic diagram at visual angle；
Fig. 3 is the structural representation at rotating-table apparatus one visual angle；
Fig. 4 is rotating-table apparatus secondly the structural representation at visual angle；
Fig. 5 is the decomposition texture schematic diagram of rotating-table apparatus；
Fig. 6 is the structural representation of rotating blade drive；
Fig. 7 is the structural representation of the gear unit of rotating blade drive；
Fig. 8 is the structural representation at clamping device one visual angle；
Fig. 9 is clamping device secondly the structural representation at visual angle；
Figure 10 is clamping device thirdly the structural representation at visual angle；
Figure 11 is the structural representation of fastener；
Figure 12 is the decomposition texture schematic diagram of fastener；
Figure 13 is the structural representation of the locking member of fastener；
Figure 14 is the structural representation of apparatus for combining liquids；
Figure 15 is the structural representation of agitating device；
Figure 16 is the structural representation of attachment means；
Figure 17 is the structural representation of autobalanced detection device；
Figure 18 is the structural representation of stabilization carrier unit；
Figure 19 is the decomposed structural representation of stabilization carrier unit；
Figure 20 is the structural representation of liquid-adding device；
Figure 21 is the structural representation of pumping device.
Embodiment
In the present description, the orientation or position relationship of the instruction such as term " on ", " under ", " preceding " and " rear " are based on attached
Orientation or position relationship shown in figure, are for only for ease of the description present invention rather than require that the present invention must be with specific orientation
Construction and operation, therefore be not considered as limiting the invention.
The embodiment to the present invention is described further below in conjunction with the accompanying drawings.
Referring to figs. 1 to Figure 21, in particular with reference to Fig. 1 and Fig. 2, a kind of cell collector, including a frame 1 are with being arranged on machine
Liquid in self-balancing rotating disk on frame 1, the liquid-adding device 5 toward adding liquid in test tube, apparatus for combining liquids and extraction test tube
Pumping device 6, self-balancing rotating disk include rotating-table apparatus 3 and for driving rotating disk device 3 rotate rotating blade drive 4, liquid
Body mixing arrangement includes some agitating devices 8 for the clamping device 7 of holding test tubes and for stirring clamping device 7, clamping
Device 7 is arranged on rotating-table apparatus 3, and agitating device 8 is arranged on the lower section of clamping device 7, and liquid-adding device 5 and pumping device 6 are set
In the top of clamping device 7.As preferred embodiment, a workbench 2 and heating tape (not shown) are provided with frame 1.
Self-balancing rotating disk passes through workbench 2 from bottom to top, the clamping device 7 on rotating-table apparatus 3 is can only see from top to bottom, it is easy to work
While the operation and observation of personnel, it is ensured that cell collector it is attractive in appearance；And heating tape ensure that the temperature of cell collector,
Advantageously ensure that the survival rate of cell collector cell during cell is collected.
Thus, the course of work of cell collector is as follows：Test tube is arranged on clamping device 7, it is past by liquid-adding device 5
Liquid feeding in test tube, agitating device 8 drives clamping device 7 to stir the liquid mixing caused in test tube, and the driving of rotating blade drive 4 turns
Disk device 3 is rotated so that clamping device 7 is rotated, so that the liquid in test tube does centrifugal motion, centrifugal motion is finished,
Pumping device 6 extracts the waste liquid in test tube, and then liquid-adding device 5 is toward liquid-adding device 5 toward liquid feeding in test tube, after being so repeated several times
Cell is completed to collect.
Referring to figs. 1 to Fig. 7, clamping device 7 is symmetrically distributed on using the rotating shaft of rotating-table apparatus 3 as the circumference in the center of circle, as
Preferred scheme, the quantity of clamping device 7 is 8, is uniformly and symmetrically distributed circumferentially.Meanwhile, self-balancing rotating disk also includes a balance
Detection means 9, when the test tube that balance detecting device 9 is detected on using the rotating shaft of rotating-table apparatus 3 as the circumference in the center of circle is symmetrical point
Cloth, rotating blade drive 4 drives rotating-table apparatus 3 to do centrifugal motion, and rotating blade drive 4 includes a centrifugation motor 43, passed
The dynamic gear unit 41 of device 42 and one, centrifugation motor 43 drives gear unit 41 to rotate by transmission device 42, gear unit
41 are connected with rotating-table apparatus 3.In addition, self-balancing rotating disk also includes liquid level detection device, liquid level detection device is to detect drawing liquid
Whether the liquid volume that device 6 is extracted from test tube is consistent, specifically, and some duplicate containers are provided with frame 1,
After pumping device 6 comes out the Liquid extracting on test tube, the liquid in each test tube is individually transferred in container, level sensing
Whether the liquid level in device detection container is consistent, if inconsistent, and cell is collected process and redo, if consistent cell is received
Collection process is consistent, so as to ensure that the liquid volume in clamping device 7 in test tube is the same, so as to ensure that self-balancing rotating disk is turning
Balance in dynamic process, with reach cell collect during liquid in each test tube by identical centrifugal action, from
And ensure the uniformity of result.
As the presently preferred embodiments, reference picture 3 is to Fig. 5, and rotating-table apparatus 3 includes a housing 31, turning in housing 31
Disk 32, rotating disk mounting seat 33, the mounting bracket 35 for installing gear unit 41.Housing 31 is arranged in frame 1, and the side of housing 31
While being provided with some air vents 311, hot gas is blown into air vent 311, it is ensured that and the temperature of regulation cell collector, further
Ensure the survival rate of cell collector cell during cell is collected.Rotating disk 32 is arranged in rotating disk mounting seat 33, rotating disk
Mounting seat 33 is connected to one with the output shaft 411 of gear unit 41 by way of motion thread hole 413 is matched with drive screw
Rise, meanwhile, the side of rotating disk mounting seat 33 is provided with a hollow boss matched with output shaft 411 and a gear unit 41 is pacified
Bearing 34 is filled, to be further ensured that reliability that rotating disk mounting seat 33 is connected with output shaft 411, it is ensured that output shaft 411 turns at a high speed
The stability and balance of rotating disk 32 when dynamic.In addition, balance positioner is provided with rotating-table apparatus 3, when active cell is collected
During instrument, balance positioner causes gear unit 41 to go to the angle of setting in order to which balance detecting device 9 starts to detect rotating disk
On 32 in test tube liquid distribution situation.Balance positioner is arranged on gear unit 41 including one and installs infrared on bearing 34
Line equipment 36 and the infrared ray catch 37 being arranged in rotating disk mounting seat 33.
As the presently preferred embodiments, reference picture 6 is to Fig. 7, and centrifugation motor 43 is arranged in frame 1, the preferred skin of transmission device 42
V belt translation mode, gear unit 41 by dynamic head main spindle structure, gear unit 41 include input shaft 415, bearing of input shaft
414th, output shaft 411, be provided with motion thread hole 413, the diameter of axle of output shaft 411 on output shaft bearing 412, and output shaft 411
More than input shaft 414, gear unit 41 ensure that output shaft 411 turns in high speed well by the structure of dynamic head main spindle
Good balance and stability is remained to when dynamic.
Reference picture 8 is to Figure 10, and clamping device 7, which includes a mounting seat 71, a swing component 72 and one, to be used to carry test tube
Fastener, mounting seat 71 is arranged on rotating-table apparatus 3, and swing component 72 is connected with mounting seat 71 by the first swinging axle 74, is made
Swing component 72 can be mounted opposite seat 71 and swing, fastener is connected with swing component 72 by the second swinging axle 75 so that fastened
Device can opposing oscillatory part 72 swing, and fastener is connected with agitating device 8.Preferably, the first swinging axle 74 with
Second swinging axle 75 is in vertical distribution.Because clamping device 7 is provided with the first swinging axle 74 and the second swinging axle 75, when stirring dress
When putting the swing of 8 drive fasteners, fastener opposing oscillatory part 72 is swung, and swing component 72 is mounted opposite seat 71 and swung, and by
It is different in the direction of both opposing oscillatories so that the liquid in test tube is fully stirred, it is ensured that cell is sufficiently mixed.
Meanwhile, in order to ensure that clamping device 7 has more preferable stirring effect under the drive of agitating device 8, set in the lower end of swing component 72
Balancing weight 76 is equipped with, balancing weight 76 is arranged on the lower section of the first swinging axle 74 and the second swinging axle 75；In order to ensure clamping device 7
In test tube during high speed centrifugation motion is done, it is to avoid the amplitude of oscillation is excessive because of centrifugalization for swing component 72, causes test tube
In liquid outflow test tube, centrifugation postive stop baffle 711 is provided with mounting seat 71, it is ensured that the amplitude of oscillation of swing component 72 is no more than 90 °.
Reference picture 11 is to Figure 13, and fastener includes the rack for test tube 73 for being used to carry test tube, and rack for test tube 73 is in cylinder, examination
Some mounting holes 77 are symmetrically arranged with the circumference of pipe support 73, the diameter of mounting hole 77, which is less than, sets the outer of thread on test tube outer wall
Footpath, test tube is so as in mounting hole 77.Preferably, the quantity of mounting hole 77 is 8, and is evenly distributed on circle
Zhou Shang.
Especially, in order to prevent that, in 3 high-speed rotation of rotating-table apparatus, the test tube on rack for test tube 73 is thrown out of, fastening dress
Putting also includes a locking device for being used to lock test tube, and locking device, which includes a locking lid 781, one, makes locking lid 781 and test tube
Frame 73 keeps the locking bed 787 and one of certain distance that locking lid 781 is locked to the locking member 783 on rack for test tube 73.Locking
Lid 781 is to be provided with to open less than test tube with the corresponding through hole of mounting hole 77, the diameter of the through hole in a cylinder, locking lid 781
The external diameter at mouth end, so that during preventing that swing component 72 from swinging, test tube takes off from mounting hole 77, and locking lid 781
Middle part be provided with a locking through hole, when locking lid 781 is locked on locking bed 787 by locking member 783, locking lid 781
Lower surface is against the open end of test tube.Locking bed 787 is fixedly mounted on the upper center of rack for test tube 73, the middle part of locking bed 787
It is provided with ladder hole, ladder hole and is provided with an attachment screw 786.Locking member 783 includes a main body 7831, main body 7831
One end be provided with the screwed hole matched with attachment screw 786 so that locking member 783 can shoulder hole slide but can not skid off,
The other end of main body 7831 is provided with a tight lock part for exposing locking bed 787, and tight lock part includes some mutually with certain intervals
Elastomer block 7832, the outer middle side part of elastomer block 7832 is outwardly, preferably, and the quantity of elastomer block 7832 is 4, elasticity
The outer wall of block 7832 for "<" type.When not having external force effect, tight lock part is more than locking in the flowers are in blossom shape, the periphery at the middle part of elastomer block 7832
The diameter of through hole so that locking lid 781 cannot pass through tight lock part, so as to which locking lid 781 is pinned；In the presence of external force,
Elastomer block 7832 is to contract so that the periphery at the middle part of elastomer block 7832 is less than the diameter of locking through hole so that the energy of locking lid 781
Through tight lock part, so as to which locking lid 781 is opened；Remove after external force, elastomer block 7832 is in the presence of elastic force to abduction
Open, recover the flowers are in blossom shape.Preferably, a spring (not shown) is provided between attachment screw 786 and rack for test tube 73,
So when locking member 783 pins locking lid 781, locking member 783 is pressed directly down, you can so that elastomer block 7832 is inside
Shrink, so as to open locking lid 781.In addition, locking device also includes a support block 785 being arranged on rack for test tube 73 and connected
Block 784 is connect, locking lid 781 can be rotated by the shaft connecting mode of bolt and nut 782 relative to contiguous block 784.
Reference picture 14 is provided with an attachment means to Figure 16 between agitating device 8 and clamping device 7, tried when needing to stir
During liquid in pipe, agitating device 8 is connected with clamping device 7, after the aqueous agitation in test tube is finished, agitating device 8 and folder
Hold device 7 to separate, attachment means are arranged on clamping device 7 including a female joint 81 and one being arranged on agitating device 8
Pin end 792.
Preferably, female joint 81 is " U " type structure, and it is arranged on a pedestal 82, and pin end 792 is a circle
Rod structure, its external diameter and the matching grooves of female joint 81 " U " type structure, and height of the depth more than the cylindrical structure of the groove
Degree, pin end 792 is connected by an elongated swing arm 791 with fastener, and can be slided up and down along swing arm 791.Swing arm
791 lower end is provided with a lock bolt 793 for preventing pin end 792 from skidding off, while it is public that a restriction is provided with swing arm 791
The block 794 of the upward sliding of joint 792.Move up when pedestal 82 moves up drive female joint 81 and will wrap pin end 792
When, agitating device 8 completes to be connected with clamping device 7.During connection, even if female joint 81 and the axle center of pin end 792
In the presence of certain deviation, because pin end 792 can move up a certain distance, female joint 81 still can successfully wrap public access
First 792 so that operation becomes simple, efficient.In addition, preferably, female joint 81 can be with opposite base 82 around its axle
Line is rotated, and so when agitating device 8 does eccentric circular motion, can reduce the abrasion between female joint 81 and pin end 792.
Preferably, agitating device 8 includes an eccentric wheel 83 and the stirring motor rotated with movable eccentric wheel 83 (figure
Not shown in), pedestal 82 is arranged on eccentric wheel 83 so that stirring motor can drive female joint 81 to do eccentric circular motion.It is excellent
Choosing, a connection locator is provided with agitating device 8, when agitating device 8 needs to be connected with clamping device 7, connection is fixed
Position device causes stirring motor to go to set angle so that the axis of female joint 81 is overlapped with the axis of pin end 792.Connection is fixed
The position preferred optoelectronic induction sensing device of device is controlled to stirring motor control parameter so that when stirring motor starts,
It is automatically brought to set angle.Stirring motor is when band moving base 82 does eccentric motion, due between pin end 792 and female joint 81
With certain gap, simultaneously because the meeting in the case where the effect of centrifugation interacts with female joint 81 with pin end 792 of female joint 81
Rotation, when the drive pin end 792 of female joint 81 does eccentric motion, female joint 81 can beat pin end 792 at random, in addition tightly
It is fixedly mounted with and puts and can be rotated relative to the first swinging axle 74 with the second swinging axle 75 so that the liquid in fastener can cmpletely
Mixing.
Reference picture 17, balance detecting device 9 includes a base 91, some support bars 92 being arranged on base 91, support
Set respectively on the quantity of bar 92 and position and the quantity of installation screwed hole 77 and position consistency on clamping device 7, support bar 92
There is a detection whether to install in screwed hole 77 equipped with the sensor 93 for whether having liquid in test tube and test tube.It is used as preferred side
Case, balance detecting device 9 also includes being connected positioning joint 94, the connection of positioning joint 94 and pin end 792 with a pin end 792
Tired state is done as the connection of pin end 792, not with female joint 81.The setting of positioning joint 94 can guarantee that balance detecting device 9
During work, clamping device 7 is not moved.
Reference picture 18 is to Figure 21, the invention discloses a kind of stabilization toter, including at least one stabilization carrier unit
10, stabilization carrier unit 10 includes a guide rail 102, the sliding block 104 being slidably arranged on guide rail 102, the gas moved with movable slider
The direction moved on cylinder 101, guide rail 102 along sliding block 104, which is provided with raised line 103, sliding block 104, is provided with a delivery platform 105, fortune
It is provided with microscope carrier 105 on bootstrap block 106, bootstrap block 106 and sets fluted, the groove is matched with raised line 103 so that work as cylinder
During 101 reciprocating motion, the linear motion for delivering platform 105 is more steady.
As a kind of embodiment of stabilization toter, reference picture 20, it is single that stabilization toter includes a stabilization delivery
Member 10, guide rail 102 is vertically arranged, and a horizontal rotary mechanism is provided with delivery platform 105, and horizontal rotary mechanism includes an electric rotating
Machine 51 and one is arranged on the level frame 52 of the output end of electric rotating machine 51.The two ends of level frame 52 are symmetrically arranged with liquid feeding convolution
Mechanism, the liquid feeding convolution motor 53 that liquid feeding cyclotron mechanism includes liquid-feeding tube 54 and drives liquid-feeding tube 54 to circle round.Stabilization toter
The corresponding organization names of the embodiment be referred to as to have used stabilization carrier unit 10 in liquid-adding device 5, liquid-adding device 5 so that
Liquid-feeding tube 54 is more steady toward what is moved during test tube adding liquid.
As another embodiment of stabilization toter, reference picture 21, stabilization toter stabilization carrier mechanism includes
Two stabilization carrier units 10, one is referred to as horizontal rectilinear motion mechanism 10 ' ', secondly it is referred to as vertically movable mechanism 10 ',
The guide rail of horizontal rectilinear motion mechanism 10 ' ' is horizontally disposed with, and the guide rail of vertically movable mechanism 10 ' is vertically arranged, and vertically movable machine
The guide rail of structure 10 ' is arranged on the delivery platform of horizontal rectilinear motion mechanism.One is provided with the delivery platform of vertically movable mechanism 10 '
Drawing liquid cyclotron mechanism, the drawing liquid convolution motor 61 that drawing liquid cyclotron mechanism includes liquid suction pipe 62 and drives liquid suction pipe 62 to circle round.Stabilization
The corresponding organization names of the embodiment of toter are referred to as to have used stabilization carrier unit in pumping device 6, pumping device 6
10 so that liquid suction pipe
The announcement and teaching of book according to the above description, those skilled in the art in the invention can also be to above-mentioned embodiment party
Formula is changed and changed.Therefore, the invention is not limited in embodiment disclosed and described above, to the present invention's
Some modifications and changes should also be as falling into the scope of the claims of the present invention.Although in addition, being used in this specification
Some specific terms, but these terms are merely for convenience of description, do not constitute any limitation to the present invention.
Claims (10)
1. a kind of cell collector, it is characterised in that：It includes a frame and the self-balancing rotating disk, past being arranged in the frame
The liquid-adding device of adding liquid, apparatus for combining liquids and the pumping device for extracting liquid in test tube, the self-balancing in test tube
Rotating disk includes rotating-table apparatus and the rotating blade drive for driving the rotating-table apparatus to rotate, and the apparatus for combining liquids includes
Some agitating devices for the clamping device of holding test tubes and for stirring the clamping device, the clamping device is arranged on
On the rotating-table apparatus, the agitating device is arranged on below the clamping device, and the liquid-adding device and pumping device are set
Above the clamping device.
2. cell collector as claimed in claim 1, it is characterised in that：The clamping device includes a mounting seat, a swing
Part and a fastener for carrying test tube, the mounting seat are arranged on the rotating-table apparatus, the swing component and peace
Dress seat connect by the first swinging axle so that swing component can be mounted opposite seat swing, and the fastener and swing component pass through the
Two swinging axles are connected so that fastener can opposing oscillatory part swing, and the fastener is connected with the agitating device.
3. cell collector as claimed in claim 2, it is characterised in that：First swinging axle and the second swinging axle are perpendicular
Distribution.
4. the cell collector as described in claim any one of 1-3, it is characterised in that：The agitating device and clamping device it
Between be provided with an attachment means, when need stir test tube in liquid when, agitating device is connected with clamping device, when in test tube
After aqueous agitation is finished, agitating device is separated with clamping device, and the attachment means are arranged on the agitating device including one
Female joint be arranged on pin end on the clamping device with one.
5. cell collector as claimed in claim 4, it is characterised in that：The agitating device includes an eccentric wheel and drive
The stirring motor that the eccentric wheel is rotated, the female joint is set on the eccentric wheel so that the stirring motor can drive institute
State female joint and do eccentric circular motion.
6. cell collector as claimed in claim 5, it is characterised in that：Connection positioning dress is provided with the agitating device
Put, when the agitating device is connected with clamping device, connection locator causes the stirring motor to go to set angle.
7. the cell collector as described in claim any one of 1-3, it is characterised in that：The clamping device be symmetrically distributed in
The rotating shaft of the rotating-table apparatus is on the circumference in the center of circle.
8. cell collector as claimed in claim 7, it is characterised in that：The self-balancing rotating disk is filled including a balance detection
Put, when the test tube that the balance detecting device is detected on using the rotating shaft of the rotating-table apparatus as the circumference in the center of circle is symmetrical
, the rotating blade drive drives the rotating-table apparatus to do centrifugal motion.
9. cell collector as claimed in claim 8, it is characterised in that：It also includes liquid level detection device, the liquid level inspection
Whether the liquid volume that survey device is extracted to detect from test tube is consistent.
10. cell collector as claimed in claim 1, it is characterised in that：The rotating blade drive include one centrifugation motor,
Transmission device and a gear unit, the centrifugation motor drive the gear unit to rotate by the transmission device, described
Gear unit is connected with the rotating-table apparatus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710492615.4A CN107189931A (en) | 2017-06-26 | 2017-06-26 | A kind of cell collector |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710492615.4A CN107189931A (en) | 2017-06-26 | 2017-06-26 | A kind of cell collector |
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| CN107189931A true CN107189931A (en) | 2017-09-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710492615.4A Pending CN107189931A (en) | 2017-06-26 | 2017-06-26 | A kind of cell collector |
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| Country | Link |
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| CN (1) | CN107189931A (en) |
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| CN108311038A (en) * | 2018-03-18 | 2018-07-24 | 钟建筑 | A kind of medicine test tube reagent evenly mixing device |
| CN109628287A (en) * | 2018-12-28 | 2019-04-16 | 科兴（大连）疫苗技术有限公司 | Cell collects shaking table and cell collection method |
| CN110724624A (en) * | 2018-11-22 | 2020-01-24 | 陈双双 | Cell recovery device |
| CN114433364A (en) * | 2022-04-07 | 2022-05-06 | 深圳人体密码基因科技有限公司 | Centrifugal equipment for adjusting radius of rotor for gene detection |
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| CN108311038A (en) * | 2018-03-18 | 2018-07-24 | 钟建筑 | A kind of medicine test tube reagent evenly mixing device |
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| CN114433364B (en) * | 2022-04-07 | 2022-06-17 | 深圳人体密码基因科技有限公司 | Centrifugal equipment for adjusting radius of rotor for gene detection |
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