CN113999771B

CN113999771B - Immature dendritic cell recovery device

Info

Publication number: CN113999771B
Application number: CN202111650561.2A
Authority: CN
Inventors: 李春雨; 纪元; 王学动; 闫奔; 任美佳; 覃婷; 王志媛; 冯丽敏; 张静
Original assignee: Maisaikele Shandong Life Science Co ltd
Current assignee: Maisaikele Shandong Life Science Co ltd
Priority date: 2021-12-31
Filing date: 2021-12-31
Publication date: 2022-04-08
Anticipated expiration: 2041-12-31
Also published as: CN113999771A

Abstract

The invention discloses an immature dendritic cell resuscitation device, which relates to the technical field of biological experiment equipment and comprises a base, wherein the left side of the top of the base is connected with a fixed seat, the right side of the top of the base is connected with a heat insulation shell, the right side wall of the fixed seat is provided with a guide groove, a screw rod is rotationally connected in the guide groove, the upper end of the screw rod is provided with a driving motor, the outer wall of the screw rod is in threaded connection with a nut seat, the right end of the nut seat is connected with a supporting table, the right part of the supporting table is rotationally connected with a deflection shaft, the lower end of the deflection shaft is connected with a U-shaped seat, and the lower end of the U-shaped seat is detachably provided with a positioning table; the device has the advantages of reasonable structural design, simple operation and high automation degree, does not need manual hand-held oscillation, reduces the labor intensity and improves the recovery efficiency of immature dendritic cells.

Description

Immature dendritic cell recovery device

Technical Field

The invention relates to the technical field of biological experimental equipment, in particular to an immature dendritic cell recovery device.

Background

Dendritic cells are known to be antigen presenting cells with the strongest functions at present, and are nerve cells, and the dendritic cells are named because a plurality of dendritic-like or pseudopodous-like protrusions extend out when the dendritic cells are mature, the starting of cell experiments usually needs to culture and passage the frozen cells after cell recovery, but the cell recovery is carried out by manual operation at present, the existing method for recovering the cells manually is to tightly clamp a freezing tube which is provided with a cell specimen in a liquid nitrogen tube by using tweezers, the taken freezing tube is placed in a preheated water bath kettle at 37 ℃ to carry out violent back-and-forth oscillation and shaking for a plurality of minutes, and when the cells in the freezing tube are completely dissolved, the cell recovery is completed. However, the long-time violent shaking of the artificial resuscitation cells often causes weakness of wrists of workers, further easily causes that the cryopreservation tube is immersed below the water surface, and easily causes pollution and damage to the cells, and therefore a novel immature dendritic cell resuscitation device is provided.

Disclosure of Invention

The invention aims to provide an immature dendritic cell recovery device, which solves the existing technical problems.

In order to achieve the technical purpose and achieve the technical effect, the invention provides the following technical scheme:

an immature dendritic cell recovery device comprises a base, wherein the left side of the top of the base is connected with a fixed seat, the right side of the top of the base is connected with a heat insulation shell, the right side wall of the fixed seat is provided with a guide groove, a lead screw is rotationally connected in the guide groove, the upper end of the lead screw is provided with a driving motor, the outer wall of the lead screw is in threaded connection with a nut seat, the right end of the nut seat is connected with a supporting table, the right part of the supporting table is rotationally connected with a deflection shaft, the lower end of the deflection shaft is connected with a U-shaped seat, and the lower end of the U-shaped seat is detachably provided with a positioning table;

the right side at the top of brace table is connected with the transmission shell, the one end that the deflection axle stretched into the transmission shell is connected with the straight-teeth gear, the spur rack is connected in the rear end meshing of straight-teeth gear, the left end of spur rack is connected with reciprocating rod, the guiding hole that supplies reciprocating rod to pass is seted up to transmission shell left end, the top left side of brace table inlays and is equipped with steering motor, steering motor's top power take off end is connected with the carousel, the top of carousel articulates through first round pin axle has the traction lever, the right-hand member of traction lever passes through the articulated reciprocating rod of second round pin axle.

Preferably, in the immature dendritic cell resuscitator, the locating platform has a plurality of locating holes along vertical direction through, it has the cell test tube to peg graft in the locating hole, the inner wall bottom of locating hole is connected with the sealing washer, the storage tank has been seted up in the inner wall middle section of locating hole, the embedded dehumidification cotton layer that is equipped with of storage tank, the notch has been seted up on the inner wall upper portion of locating hole, the notch in-connection has reset spring, reset spring's expansion end is connected with the arc piece, arc piece butt cell test tube outer wall, the spout has been seted up at both ends about the locating platform, the upper end of spout has run through the anticreep hole.

Preferably, in the immature dendritic cell resuscitator, the perpendicular portion inner wall of U type seat is connected with the cutting, cutting activity grafting spout, the horizontal portion inner wall of U type seat is connected with electric putter one, electric putter one's bottom is connected with the buckler, the outer wall left and right sides of buckler is connected with L shape pin rod, the perpendicular end of L shape pin rod stretches into the anticreep hole.

Preferably, in the immature dendritic cell resuscitating device, the bottom left side of the supporting platform is connected with a triangular seat, the left end of the triangular seat is provided with a universal ball head, and the universal ball head is abutted to the right side wall of the fixing seat.

Preferably, in an immature dendritic cell resuscitator, the inner chamber bottom of thermal-insulated shell is connected with the mounting bracket, the upper end of mounting bracket is connected with the water bath, constant temperature heating ware is installed to the inner chamber bottom of water bath, the water bath is located the location platform under, bilateral symmetry articulates around the top of thermal-insulated shell has half opening the door, two half opening the door between the symmetry has been seted up the semicircle mouth, the deflection axis runs through the semicircle mouth, it has electric putter two to articulate between half opening the door and the thermal-insulated shell outer wall.

Preferably, in the immature dendritic cell recovery device, moving wheels are installed at four corners of the bottom of the base, and brake pads are arranged on the moving wheels.

Compared with the prior art, the invention has the beneficial effects that:

1. the device has the advantages of reasonable structural design, simple operation and high automation degree, does not need manual hand-held oscillation, reduces the labor intensity and improves the recovery efficiency of immature dendritic cells;

2. the invention is convenient to move, the constant temperature heater is used for heating the clear water at constant temperature, the screw rod rotates, the nut seat moves downwards along the guide groove, the depth of the cell test tube on the positioning table entering the liquid level of the water bath box is convenient to control, the electric push rod II is used for driving the half-open door to deflect, the half-open door leans against the outer part of the deflection shaft, the upper end of the water bath box can be covered, the heat loss is reduced, and the energy-saving effect is better;

3. according to the invention, the arc block is tightly attached to the pipe wall by utilizing the elastic action of the return spring, clamping and fixing can be carried out, the sliding groove extends into along the inserting strip, the positioning table enters the lower end of the opening of the U-shaped seat, the L-shaped pin rod extends into the anti-falling hole when the electric push rod drives the waterproof cover to descend, and the installation of the positioning table can be rapidly completed;

4. according to the invention, the steering motor drives the turntable to rotate, the reciprocating rod is pushed and pulled in a left-right reciprocating mode at the right end of the traction rod, the spur rack is used for meshing and driving the spur gear, and the cell test tube can oscillate back and forth in the liquid level after the deflection shaft deflects in a reciprocating mode, so that the recovery progress of immature dendritic cells is accelerated.

Drawings

In order to more clearly illustrate the technical solution of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained based on these drawings without inventive labor.

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a side view structural diagram of the present invention;

FIG. 3 is a schematic view of the internal structure of the present invention;

FIG. 4 is a schematic top view of a support stage according to the present invention.

FIG. 5 is a schematic view of the internal structure of the positioning table of the present invention.

In the figure: 1. a base; 2. a fixed seat; 3. a thermally insulating shell; 4. a guide groove; 5. a screw rod; 6. a drive motor; 7. a nut seat; 8. a support table; 9. a yaw axis; 10. a U-shaped seat; 11. a positioning table; 12. a drive housing; 13. a spur gear; 14. straight rack; 15. a reciprocating lever; 16. a guide hole; 17. a steering motor; 18. a turntable; 19. a first pin shaft; 20. a draw bar; 21. a second pin shaft; 301. a mounting frame; 302. a water bath tank; 303. a constant temperature heater; 304. half-opening the door; 305. a semicircular opening; 306. a second electric push rod; 801. a triangular base; 802. a universal ball head; 101. cutting; 102. an electric push rod I; 103. a waterproof cover; 104. an L-shaped pin; 111. positioning holes; 112. a seal ring; 113. a containing groove; 114. a layer of moisture-removing cotton; 115. a recess; 116. a return spring; 117. an arc block; 118. a chute; 119. and (5) anti-drop holes.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example one

Referring to fig. 1-5, the present embodiment is an immature dendritic cell resuscitating device, which includes a base 1, a fixing base 2 is connected to the left side of the top of the base 1, a heat insulation shell 3 is connected to the right side of the top of the base 1, a guide groove 4 is formed in the right side wall of the fixing base 2, a screw rod 5 is rotatably connected to the guide groove 4, a driving motor 6 is installed at the upper end of the screw rod 5, a nut seat 7 is connected to the outer wall of the screw rod 5 through a thread, a supporting table 8 is connected to the right end of the nut seat 7, a deflection shaft 9 is rotatably connected to the right portion of the supporting table 8, a U-shaped seat 10 is connected to the lower end of the deflection shaft 9, and a positioning table 11 is detachably installed at the lower end of the U-shaped seat 10.

The inner chamber bottom of thermal-insulated shell 3 is connected with mounting bracket 301, and the upper end of mounting bracket 301 is connected with water bath 302, and constant temperature heating ware 303 is installed to the inner chamber bottom of water bath 302, and water bath 302 is located the location platform 11 under, and bilateral symmetry articulates around the top of thermal-insulated shell 3 has half opening door 304, and half mouth 305 has been seted up to the symmetry between two half opening doors 304, and deflection axle 9 runs through half mouth 305, and it has two electric putter 306 to articulate between half opening door 304 and the thermal-insulated shell 3 outer wall.

The bottom four corners of base 1 is installed and is removed the wheel, removes to take turns to and is equipped with the brake block, is convenient for remove the device.

The specific implementation manner of this embodiment is:

this device external power supply when using, utilize to remove the wheel and remove base 1 to work area, carry out spacing fixed to the device through the brake block, pour into quantitative clear water into water bath 302, heat the clear water to 37 ℃ through constant temperature heating ware 303, utilize locating platform 11 to fix the cell test tube that carries immature dendritic cell, it rotates to drive lead screw 5 through driving motor 6, nut seat 7 can be followed guide way 4 downstream, brace table 8 descends along with nut seat 7 is synchronous, make locating platform 11 get into water bath 302 in, drive half-open door 304 through electric putter two 306 and deflect, two half-openings 305 are to leaning on in deflection shaft 9 outsidely, the cell test tube gets into in the liquid, half-open door 304 can cover water bath 302 upper end, reduce the heat and scatter, be convenient for carry out the thawing recovery of immature dendritic cell.

Example two

On the basis of the first embodiment, the right side of the top of the supporting table 8 is connected with a transmission shell 12, one end, extending into the transmission shell 12, of the deflection shaft 9 is connected with a spur gear 13, the rear end of the spur gear 13 is connected with a spur rack 14 in a meshed mode, the left end of the spur rack 14 is connected with a reciprocating rod 15, the left end of the transmission shell 12 is provided with a guide hole 16 through which the reciprocating rod 15 penetrates, the left side of the top of the supporting table 8 is embedded with a steering motor 17, the top power output end of the steering motor 17 is connected with a rotary table 18, the top of the rotary table 18 is hinged to a traction rod 20 through a first pin shaft 19, and the right end of the traction rod 20 is hinged to the reciprocating rod 15 through a second pin shaft 21.

Locating station 11 has run through along vertical direction has a plurality of locating holes 111, the cell test tube has been inserted to the locating hole 111 interpolation, the inner wall bottom of locating hole 111 is connected with sealing washer 112, storage tank 113 has been seted up in the inner wall middle section of locating hole 111, the embedded cotton layer 114 that dehumidifies that is equipped with of storage tank 113, notch 115 has been seted up on the inner wall upper portion of locating hole 111, notch 115 in-connection has reset spring 116, reset spring 116's expansion end is connected with arc piece 117, arc piece 117 butt cell test tube outer wall, spout 118 has been seted up at both ends about locating station 11, spout 118's upper end has run through anticreep hole 119.

The vertical portion inner wall of U type seat 10 is connected with cutting 101, and cutting 101 activity grafting spout 118, the horizontal portion inner wall of U type seat 10 are connected with electric putter 102, and the bottom of electric putter 102 is connected with buckler 103, and the outer wall left and right sides of buckler 103 is connected with L shape pin 104, and the vertical end of L shape pin 104 stretches into anticreep hole 119.

The bottom left side of brace table 8 is connected with triangle seat 801, and universal bulb 802 is installed to the left end of triangle seat 801, and universal bulb 802 butt 2 right side walls of fixing base can carry out auxiliary stay to brace table 8.

The specific implementation manner of this embodiment is:

in the embodiment, the cell test tube extends into the positioning hole 111, the arc block 117 is tightly attached to the tube wall by the elastic force of the return spring 116, the cell test tube can be clamped and fixed, the water flow is prevented from invading through the sealing ring 112, the dehumidifying cotton layer 114 is embedded in the accommodating groove 113, the residual water drops on the outer wall can be removed when the cell test tube is taken out, the sliding groove 118 extends into the sliding strip 101, the positioning table 11 enters the lower end of the opening of the U-shaped seat 10, the waterproof cover 103 is driven to descend through the electric push rod one 102, the vertical end of the L-shaped pin rod 104 extends into the anti-falling hole 119, the waterproof cover 103 abuts against the upper end of the positioning table 11, and the fixing of the positioning table 11 can be completed quickly;

the rotating disc 18 is driven to rotate by the steering motor 17, the left end of the traction rod 20 makes circular motion along with the first pin shaft 19, the reciprocating rod 15 is guided by the guide hole, the reciprocating rod 15 can be pushed and pulled in a left-right reciprocating mode by the right end of the traction rod 20, the straight rack 14 moves synchronously along with the reciprocating rod 15, the straight rack 14 is meshed with the transmission straight gear 13, the straight gear 13 drives the deflection shaft 9 to deflect in a reciprocating mode, the U-shaped seat 10 drives the positioning table 11 to deflect, cell test tubes can oscillate back and forth in the liquid level, and the recovery progress of immature dendritic cells is accelerated.

In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. An immature dendritic cell resuscitation device comprising a base (1), characterized in that: the heat insulation device is characterized in that a fixed seat (2) is connected to the left side of the top of the base (1), a heat insulation shell (3) is connected to the right side of the top of the base (1), a guide groove (4) is formed in the right side wall of the fixed seat (2), a lead screw (5) is connected in the guide groove (4) in a rotating mode, a driving motor (6) is installed at the upper end of the lead screw (5), a nut seat (7) is connected to the outer wall of the lead screw (5) in a threaded mode, a supporting table (8) is connected to the right end of the nut seat (7), a deflection shaft (9) is connected to the right portion of the supporting table (8) in a rotating mode, the lower end of the deflection shaft (9) is connected with a U-shaped seat (10), and a positioning table (11) is detachably installed at the lower end of the U-shaped seat (10);

the right side of the top of the supporting table (8) is connected with a transmission shell (12), one end, extending into the transmission shell (12), of the deflection shaft (9) is connected with a straight gear (13), the rear end of the straight gear (13) is meshed with a straight rack (14), the left end of the straight rack (14) is connected with a reciprocating rod (15), the left end of the transmission shell (12) is provided with a guide hole (16) for the reciprocating rod (15) to penetrate through, the left side of the top of the supporting table (8) is embedded with a steering motor (17), the top power output end of the steering motor (17) is connected with a rotary table (18), the top of the rotary table (18) is hinged with a traction rod (20) through a first pin shaft (19), and the right end of the traction rod (20) is hinged with the reciprocating rod (15) through a second pin shaft (21);

the cell test tube positioning device is characterized in that a plurality of positioning holes (111) penetrate through the positioning table (11) in the vertical direction, cell test tubes are inserted into the positioning holes (111), sealing rings (112) are connected to the bottoms of the inner walls of the positioning holes (111), a containing groove (113) is formed in the middle section of the inner wall of the positioning holes (111), a dehumidifying cotton layer (114) is embedded into the containing groove (113), notches (115) are formed in the upper portions of the inner walls of the positioning holes (111), return springs (116) are connected into the notches (115), the movable ends of the return springs (116) are connected with arc blocks (117), the arc blocks (117) abut against the outer walls of the cell test tubes, sliding grooves (118) are formed in the left end and the right end of the positioning table (11), and anti-falling holes (119) penetrate through the upper ends of the sliding grooves (118);

an inserting strip (101) is connected to the inner wall of the vertical portion of the U-shaped seat (10), a sliding groove (118) is movably inserted into the inserting strip (101), a first electric push rod (102) is connected to the inner wall of the horizontal portion of the U-shaped seat (10), a waterproof cover (103) is connected to the bottom of the first electric push rod (102), L-shaped pin rods (104) are connected to the left side and the right side of the outer wall of the waterproof cover (103), and the vertical end of each L-shaped pin rod (104) extends into an anti-falling hole (119);

the bottom left side of brace table (8) is connected with triangular seat (801), universal bulb (802) are installed to the left end of triangular seat (801), universal bulb (802) butt fixing base (2) right side wall.

2. The immature dendritic cell resuscitation device of claim 1, wherein: the inner chamber bottom of thermal-insulated shell (3) is connected with mounting bracket (301), the upper end of mounting bracket (301) is connected with water bath (302), constant temperature heating ware (303) are installed to the inner chamber bottom of water bath (302), water bath (302) are located location platform (11) under, bilateral symmetry articulates around the top of thermal-insulated shell (3) has half-open door (304), two half-opening door (304) between the symmetry seted up semicircle orifice (305), deflection axle (9) run through semicircle orifice (305), it has electric putter two (306) to articulate between half-open door (304) and thermal-insulated shell (3) outer wall.

3. The immature dendritic cell resuscitation device of claim 1, wherein: the bottom four corners of base (1) are installed and are removed the wheel, it is equipped with the brake block to remove to take turns.