CN210945571U

CN210945571U - Stem cell recovery device

Info

Publication number: CN210945571U
Application number: CN201920832258.6U
Authority: CN
Inventors: 韩瑛璐; 唐健; 朱旭石
Original assignee: Aixin Suzhou Life Science Co ltd
Current assignee: Chengxi Shanghai Biotechnology Co ltd
Priority date: 2019-06-04
Filing date: 2019-06-04
Publication date: 2020-07-07
Anticipated expiration: 2029-06-04

Abstract

The utility model discloses a stem cell resuscitation device, which comprises a base, a water bath kettle, an oscillation device, a freezing tube position adjusting device and a freezing tube fixing device, wherein the water bath kettle, the oscillation device, the freezing tube position adjusting device and the freezing tube fixing device are arranged above the base; the freezing storage pipe position adjusting device comprises a lifting mechanism and a transverse displacement adjusting mechanism; the freezing and depositing pipe fixing device comprises a connecting plate and a freezing and depositing pipe fixing plate, the freezing and depositing pipe fixing plate is fixedly connected with the lower end of the transverse displacement adjusting mechanism through the connecting plate, a plurality of clamping holes are formed in the freezing and depositing pipe fixing plate, and isolating sleeves for placing the freezing and depositing pipes and isolating the freezing and depositing pipes from liquid in the water bath pot are respectively embedded in the clamping holes; the oscillating device is arranged in the water bath kettle and is in contact with the isolation sleeve on the freezing tube fixing plate to drive the freezing tube in the isolation sleeve to oscillate. This stem cell resuscitates device makes the intraductal cell of cryopreservation be heated and the oscillation more even, and degree of automation is higher, operation safety and stability.

Description

Stem cell recovery device

Technical Field

The utility model relates to a cell biology technical field, concretely relates to stem cell resuscitator.

Background

The main subjects of cell biology studies are various primary or subcultured cells or cell lines. In order to avoid the pollution caused by the cultured cells in the long-term in-vitro culture process or the gene mutation caused by multiple passages, an experimenter can freeze the cultured cells in the vigorous growth period, the frozen cells are stored in a cell freezing tube added with a cell freezing medium and then placed in liquid nitrogen at the temperature of-196 ℃, and the cells can be stored for a long time in the ultralow temperature environment. When the experiment is needed, an experimenter can resuscitate the frozen cells in warm water at about 37 ℃ to carry out the needed experiment, so that the genetic stability of the cells can be ensured to the maximum extent.

The cell recovery is to take out the freezing tube with the frozen cells from liquid nitrogen and immerse the tube in warm water at about 37 ℃ to rapidly melt the tube, so that ice crystals frozen outside the cells are rapidly melted, the ice crystals are prevented from being slowly melted and entering the cells to be recrystallized, and the cells are prevented from being damaged due to the fact that the cells are exposed in high-concentration electrolyte and cell freezing liquid for a long time due to rapid melting of the frozen cells.

In the currently common operation of recovering cells in a freezing tube, the freezing tube is directly and integrally fixed in a water bath pot and is immersed below the liquid level in the water bath pot, so that the freezing cells are easily polluted; the conventional stem cell resuscitation device lacks an oscillating device, needs manual work to shake at intervals, is inconvenient to operate and has low oscillating efficiency; in addition, the automation degree of the existing device is low, the height of the cryopreservation tube immersed into the water bath kettle needs to be manually controlled, and the cryopreservation tube cannot be adjusted to the middle position of the water bath kettle according to the size of the water bath kettle, so that the uniform heating is ensured.

SUMMERY OF THE UTILITY MODEL

In order to solve the problem that exists among the above-mentioned background art, the utility model aims to provide a stem cell resuscitator makes the intraductal cell of cryopreserving be heated and the oscillation is more even, and degree of automation is higher, operation safety and stability.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

the utility model provides a stem cell resuscitation device, which comprises a base, a water bath kettle, an oscillation device, a freezing tube position adjusting device and a freezing tube fixing device, wherein the water bath kettle, the oscillation device, the freezing tube position adjusting device and the freezing tube fixing device are arranged above the base;

the device for adjusting the position of the freezing storage pipe comprises a lifting mechanism and a transverse displacement adjusting mechanism, wherein the lifting mechanism comprises a vertically arranged support fixed above a base, the support is positioned on one side of the water bath, a sliding groove is formed in the support towards one side surface of the water bath along the vertical direction, a sliding block is connected inside the sliding groove in a sliding mode, an electric push rod is fixedly connected above the sliding block, and the upper end of the electric push rod is fixedly connected with the upper side surface of the inner wall of the sliding groove; the transverse displacement adjusting mechanism comprises an internal balancing stand, an external balancing stand and a telescopic cylinder, the internal balancing stand, the external balancing stand and the telescopic cylinder are transversely arranged along the horizontal direction, one end of the internal balancing stand is fixed on the sliding block, the external balancing stand is sleeved on the internal balancing stand, the telescopic cylinder is fixed on the outer side of the internal balancing stand close to one end of the sliding block, the telescopic end of the telescopic cylinder is connected with the external balancing stand, and the external balancing stand slides left and right along the length direction of the internal balancing stand under the driving of the telescopic cylinder;

the freezing storage pipe fixing device comprises a connecting plate and a freezing storage pipe fixing plate, the freezing storage pipe fixing plate is fixedly connected with the lower end of the built-in balance frame through the connecting plate, a plurality of clamping holes are formed in the freezing storage pipe fixing plate, and isolating sleeves used for placing the freezing storage pipes and isolating the freezing storage pipes from liquid in the water bath pot are respectively embedded in the clamping holes;

the oscillation device is arranged in the water bath kettle, and the oscillation device is in contact with the isolation sleeve on the freezing tube fixing plate to drive the freezing tube in the isolation sleeve to oscillate.

The improved structure of the water bath kettle is characterized in that the oscillation device comprises a supporting plate, an annular fixing plate, a spring and a vibration motor, the annular fixing plate is fixed on the inner side wall of the water bath kettle, an annular groove is formed in the inner side of the annular fixing plate, the supporting plate is embedded in the annular groove, the supporting plate can move up and down in the annular groove, the vibration motor is arranged between the bottom of the supporting plate and the inner bottom of the annular groove, and the spring is arranged between the top of the supporting plate and the inner top of the annular groove. The spacer sleeve contacts with the supporting plate under the effect of elevating system and lateral displacement adjustment mechanism, opens vibrating motor, and the supporting plate vibrates from top to bottom under the effect of spring and vibrating motor, drives the spacer sleeve oscillation with the supporting plate contact, and then drives the cryopreservation pipe that is located the spacer sleeve and oscillates, and oscillation frequency and the range of oscillation in-process can be controlled through vibrating motor for it is more even to freeze intraductal cell oscillation.

The further improvement lies in that the spring with vibrating motor all is provided with three, and three the spring with three vibrating motor all is triangular distribution in space.

The improved structure is characterized in that a plurality of limiting holes are formed in one side face of the built-in balancing frame at equal intervals, a plurality of limiting pin holes corresponding to the limiting holes are formed in the external balancing frame, and the positioning pins are sequentially inserted into the limiting pin holes and the limiting holes to fix the external balancing frame. The external balancing stand is fixed at the preset horizontal displacement position by the positioning pin, and the whole device is more stable and safe to operate.

The stem cell resuscitating device further comprises a controller, a timing device and an alarm, wherein the timing device and the alarm are fixed on the support, a distance sensor is arranged on the freezing tube fixing plate, a temperature sensor is arranged in the isolation sleeve, and the distance sensor, the temperature sensor, the timing device and the alarm are respectively and electrically connected with the controller. The distance sensor can transmit the relative position of the freezing tube fixing plate bearing the freezing tube and the oscillating device in the water bath pot to the controller, the controller accurately positions the height of the freezing tube in the isolation sleeve in the water bath pot by controlling the lifting mechanism and the transverse displacement adjusting mechanism, so that the isolation sleeve is contacted with the supporting plate, meanwhile, water in the water bath pot cannot sink over the top of the isolation sleeve, and when cell resuscitation is completed, the controller controls the freezing tube fixing plate to ascend from the water bath pot to a position where an operator can conveniently operate; the temperature sensor is used for monitoring the heating temperature of the cryopreservation tube in the isolation sleeve in real time, and when the temperature is detected to be higher than a preset heating temperature value, an alarm is used for reminding an operator to process; the recovery operation of the cells in the cryopreservation tube is automatically carried out through time setting by the timer.

Compared with the prior art, the utility model discloses following beneficial effect has:

according to the invention, the freezing tube on the freezing tube fixing plate is automatically and accurately regulated and controlled to be at a proper height in the water bath pot through the lifting mechanism and the transverse displacement regulating mechanism, the freezing tube can be regulated and controlled to be positioned in the middle of the water bath pot according to the size of the water bath pot, the heating is more uniform, and the automatic cell recovery in batches is realized; by arranging the heat insulation sleeve, the cryopreservation tube is isolated from water in the water bath kettle, and cells are prevented from being polluted; through set up oscillation device in the water bath to by vibrating motor control oscillation frequency and amplitude, make the cell oscillation in the cryopreserving pipe more even. The invention realizes the automatic and batch cell recovery, improves the working efficiency, obviously improves the quality and stability of cell recovery, reduces the cell pollution probability, and has very safe and stable whole operation process.

Drawings

The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.

FIG. 1 is a perspective view of a stem cell recovery device according to example 1;

FIG. 2 is a front view of the stem cell recovery apparatus according to example 1;

FIG. 3 is an enlarged view of a portion of FIG. 2 at A;

FIG. 4 is a view showing an internal structure of a water bath in example 1;

FIG. 5 is a partial enlarged view of FIG. 4 at B;

FIG. 6 is a structural view of a lateral displacement adjusting mechanism in embodiment 2;

wherein the specific reference numerals are as follows: the device comprises a base 1, a support 2, a sliding groove 3, a sliding block 4, an electric push rod 5, an internal balancing frame 6, a limiting hole 7, an external balancing frame 8, a limiting pin hole 9, a telescopic cylinder 10, a connecting plate 11, a T-shaped groove 12, a freezing tube fixing plate 13, a T-shaped block 14, an isolating sleeve 15, a water bath kettle 16, a supporting plate 17, an oscillating device 18, an annular fixing plate 19, an annular groove 20, a spring 21 and a vibrating motor 22.

Detailed Description

Example 1

Embodiment 1 of the utility model provides a stem cell recovery device, as shown in fig. 1 and fig. 2, including base 1 and the water bath 16, the oscillation device 18, the cryopreserving pipe position adjusting device and the cryopreserving pipe fixing device of setting in base 1 top.

The device for adjusting the position of the freezing storage pipe comprises a lifting mechanism and a transverse displacement adjusting mechanism, wherein the lifting mechanism comprises a vertically arranged support 2 fixed above a base 1, the support 2 is positioned on one side of a water bath 16, a sliding groove 3 is formed in one side of the support 2 facing the water bath 16 along the vertical direction, a sliding block 4 is connected in the sliding groove 3 in a sliding manner, an electric push rod 5 is fixedly connected above the sliding block 4, and the upper end of the electric push rod 5 is fixedly connected with the upper side face of the inner wall of the sliding groove 3; lateral displacement adjustment mechanism includes along the horizontal built-in gimbal 6 that sets up of horizontal direction, external gimbal 8 and

telescopic cylinder

10, 6 one end of built-in gimbal is fixed on

slider

4, 8 covers of external gimbal are established on built-in gimbal 6, telescopic cylinder 10 is fixed in the outside that built-in gimbal 6 is close to in 4 one ends of slider, telescopic cylinder 10's flexible end is connected with external gimbal 8, external gimbal 8 carries out the horizontal slip along 6 length direction of built-in gimbal under telescopic cylinder 10's the drive.

The cryopreserved pipe fixing device comprises a connecting plate 11 and a cryopreserved pipe fixing plate 13, cryopreserved pipe fixing plate 13 passes through connecting plate 11 and the lower extreme fixed connection of built-in balancing stand 6, be equipped with a plurality of screens holes on the cryopreserved pipe fixing plate 13, inlay respectively in a plurality of screens holes and be used for placing cryopreserved pipe and with cryopreserved pipe and 16 interior liquid isolation's of water bath pot spacer sleeve 15, the specification in spacer sleeve 15 and screens hole is formed according to the specification special design of cryovial, and spacer sleeve 15 adopts the material that has excellent heat conductivility to make, the separation of liquid in cryopreserved pipe and the water bath pot 16 has both been realized, and heat conductivility is good simultaneously, it is even to have guaranteed that the intraductal cell of cryopreserved is heated.

As shown in fig. 4 and 5, the oscillating device 18 is installed in the water bath 16, and the oscillating device 18 contacts with the isolation sleeve 15 on the freezing tube fixing plate 13 to drive the freezing tube in the isolation sleeve 15 to oscillate. The oscillating device 18 comprises a supporting plate 17, an annular fixing plate 19, a spring 21 and a vibrating motor 22, the annular fixing plate 19 is fixed on the inner side wall of the water bath 16, an annular groove 20 is formed in the inner side of the annular fixing plate 19, the supporting plate 17 is embedded in the annular groove 20, the supporting plate 17 can move up and down in the annular groove 20, the vibrating motor 22 is arranged between the bottom of the supporting plate 17 and the bottom of the annular groove 20, and the spring 21 is arranged between the top of the supporting plate 17 and the top of the annular groove 20. The spacer sleeve 15 contacts with layer board 17 under elevating system and lateral displacement adjustment mechanism's effect, opens vibrating motor 22, and layer board 17 vibrates from top to bottom under spring 21 and vibrating motor 22's effect, drives the spacer sleeve 15 vibration with layer board 17 contact, and then drives the cryopreservation pipe that is located spacer sleeve 15 and vibrates, and oscillation frequency and the range of oscillation in-process can be controlled through vibrating motor 22 for cell oscillation is more even in the cryopreservation pipe. Wherein, spring 21 and vibrating motor 22 all can be provided with threely, and three spring 21 and vibrating motor 22 are triangular distribution in space, and of course, spring 21 and vibrating motor 22 also can set up to other quantity. Preferably, the supporting plate 17 is uniformly provided with protrusions on the upper surface thereof for increasing friction force.

The stem cell resuscitation device also comprises a controller, and a timing device and an alarm which are fixed on the bracket 2, wherein a freezing tube fixing plate 13 is provided with a distance sensor, a temperature sensor is arranged in the isolation sleeve 15, the distance sensor, the temperature sensor, the timing device, the alarm, the electric push rod 5 and the telescopic cylinder 10 are respectively and electrically connected with the controller, and the controller is provided with a display screen for displaying temperature, time and the like; the distance sensor can transmit the relative position of a freezing tube fixing plate 13 bearing a freezing tube and a vibration device 18 in the water bath 16 to the controller, the controller accurately positions the height of the freezing tube in the isolation sleeve 15 in the water bath 16 by controlling an electric push rod 5 in the lifting mechanism and a telescopic cylinder 10 in the transverse displacement adjusting mechanism and positions the freezing tube at the middle position in the water bath 16, so that the isolation sleeve 15 is in contact with a supporting plate 17, meanwhile, water in the water bath 16 does not sink over the top of the isolation sleeve 15, and when cell recovery is completed, the controller controls the freezing tube fixing plate 13 to ascend from the water bath 16 to a position which is convenient for an operator to operate; the temperature sensor is used for monitoring the heating temperature of the freezing storage pipe in the isolation sleeve 15 in real time, and when the temperature is detected to be higher than a preset heating temperature value, an alarm is used for reminding an operator to process; the recovery operation of the cells in the cryopreservation tube is automatically carried out through time setting by the timer.

Wherein, as shown in fig. 3, the freezing tube fixing plate 13 is detachably connected with the connecting plate 11, the connecting plate 11 is far away from one end of the built-in balance frame 6, a T-shaped groove 12 is transversely formed in the end, away from the built-in balance frame, of the connecting plate 11, a T-shaped block 14 matched with the T-shaped groove 12 is arranged on one side of the freezing tube fixing plate 13, the freezing tube fixing plate 13 matched with the freezing tube fixing plate is replaced according to the size of a freezing tube to be processed, the application range is wider, and meanwhile, the freezing tube fixing plate 13 is easy to damage and can be.

Example 2

Embodiment 2 of the utility model provides a stem cell recovery device, and embodiment 2 is the same with embodiment 1's essential feature, and both have following difference technical characteristics: as shown in fig. 6, a plurality of limiting holes 7 are arranged on one side surface of the built-in balancing stand 6 at equal intervals, a plurality of limiting pin holes 9 corresponding to the limiting holes 7 are arranged on the external balancing stand 8, and positioning pins are sequentially inserted into the limiting pin holes 9 and the limiting holes 7 to fix the external balancing stand 8; the external balancing stand 8 is fixed at the preset horizontal displacement position through the positioning pin, and the whole device is more stable and safe to operate.

It is right to have used specific individual example above the utility model discloses expound, only be used for helping to understand the utility model discloses, not be used for the restriction the utility model discloses. To the technical field of the utility model technical personnel, the foundation the utility model discloses an idea can also be made a plurality of simple deductions, warp or replacement.

Claims

1. A stem cell recovery device is characterized by comprising a base, a water bath kettle, an oscillating device, a freezing tube position adjusting device and a freezing tube fixing device, wherein the water bath kettle, the oscillating device, the freezing tube position adjusting device and the freezing tube fixing device are arranged above the base;

2. The stem cell resuscitating device of claim 1, wherein the oscillating device comprises a support plate, an annular fixing plate, a spring and a vibrating motor, the annular fixing plate is fixed on the inner side wall of the water bath, an annular groove is formed inside the annular fixing plate, the support plate is embedded in the annular groove and can move up and down in the annular groove, the vibrating motor is arranged between the bottom of the support plate and the inner bottom of the annular groove, and the spring is arranged between the top of the support plate and the inner top of the annular groove.

3. The stem cell resuscitating device of claim 2, wherein there are three springs and three vibration motors, and the three springs and the three vibration motors are distributed in a triangular space.

4. The stem cell resuscitation device according to claim 1, wherein a plurality of limiting holes are equidistantly formed in one side surface of the internal balancing stand, a plurality of limiting pin holes corresponding to the limiting holes are formed in the external balancing stand, and positioning pins are sequentially inserted into the limiting pin holes and the limiting holes to fix the external balancing stand.

5. The stem cell recovery device according to claim 1, further comprising a controller, and a timing device and an alarm fixed on the bracket, wherein a distance sensor is arranged on the freezing tube fixing plate, a temperature sensor is arranged in the isolation sleeve, and the distance sensor, the temperature sensor, the timing device and the alarm are respectively electrically connected with the controller.