CN110591900A - Cell recovery device - Google Patents
Cell recovery device Download PDFInfo
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- CN110591900A CN110591900A CN201910867559.7A CN201910867559A CN110591900A CN 110591900 A CN110591900 A CN 110591900A CN 201910867559 A CN201910867559 A CN 201910867559A CN 110591900 A CN110591900 A CN 110591900A
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- 238000011084 recovery Methods 0.000 title claims abstract description 21
- 230000005540 biological transmission Effects 0.000 claims description 9
- 238000005485 electric heating Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 24
- 239000011324 bead Substances 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 38
- 238000010586 diagram Methods 0.000 description 6
- 238000010438 heat treatment Methods 0.000 description 4
- 210000005056 cell body Anatomy 0.000 description 3
- 238000007792 addition Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/20—Heating; Cooling
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Mechanical Engineering (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to the field of cells, in particular to a cell recovery device, which can put a plurality of test tubes filled with cell solution into warm water, can swing in the front-back direction and the left-right direction in the water, can swing in a reciprocating way by taking the axis of a main shaft as a shaft, accelerates the recovery of cells in the warm water, and can fully mix the cells in a culture solution. The resuscitation pond comprises a pond body, support plates and sliding chutes, the pond body is in a rectangular box shape with an open upper end, the support plates are fixedly connected to the left end and the right end of the upper end of the pond body, and the sliding chutes are formed in the two support plates; rectangular block in the middle-end fixedly connected with of pivot, the equal fixedly connected with bead in both ends around the rectangular block of well, the left end fixedly connected with motor cabinet of pivot, the upper end fixedly connected with motor II of motor cabinet, fixedly connected with carousel II on motor II's the front end output shaft, carousel II's the articulated connecting rod II that is connected with in eccentric position.
Description
Technical Field
The invention relates to the field of cells, in particular to a cell recovery device.
Background
The invention discloses a cell resuscitator with application number CN201510694871.2, which comprises a water bath kettle, and further comprises: the lifting mechanism and the bearing device are arranged on the lifting mechanism; and the controller is electrically connected with the lifting mechanism and used for controlling the movement of the lifting mechanism and putting the bearing device into the water bath kettle. The invention realizes the automatic mass cell recovery process by combining the lifting mechanism and the controller, can accurately control the recovery time and temperature, records the recovery process parameters, avoids the possible pollution of water in the water bath on the cryopreserved cells, greatly improves the cell recovery quality, lightens the burden of operators and improves the working efficiency. However, the cell resuscitator cannot shake cells in a bath, and cannot achieve the effects of rapid resuscitation and sufficient mixing of the cells in the culture solution.
Disclosure of Invention
The invention provides a cell recovery device, which has the advantages that a plurality of test tubes filled with cell solution can be placed in warm water, the test tubes can swing in the front-back direction and the left-right direction in the water, the plurality of test tubes filled with cell solution can swing in a reciprocating mode by taking the axis of a main shaft as a shaft, the recovery of cells in the warm water is accelerated, and the cells can be fully mixed in a culture solution.
The invention relates to the field of cells, in particular to a cell recovery device which comprises a recovery pool, a lifting frame, a swinging frame and a test tube rack.
The resuscitation pond comprises a pond body, support plates and sliding chutes, the pond body is in a rectangular box shape with an open upper end, the support plates are fixedly connected to the left end and the right end of the upper end of the pond body, and the sliding chutes are formed in the two support plates;
the swing frame comprises a rotating shaft, a rotary table II, a motor base, a connecting rod II, a middle rectangular block, ribs, connecting columns and a rack II, the middle end of the rotating shaft is fixedly connected with the middle rectangular block, the front end and the rear end of the middle rectangular block are fixedly connected with the ribs, the left end of the rotating shaft is fixedly connected with the motor base, the upper end of the motor base is fixedly connected with the motor II, the front end output shaft of the motor II is fixedly connected with the rotary table II, the eccentric position of the rotary table II is hinged with the connecting rod II, the right end of the rotating shaft is fixedly connected with the connecting column, the rack II is fixedly connected onto the connecting column;
the test-tube rack includes the diaphragm, gear II, gear III, front and back flitch, articulated footstock, L shape is board even, main shaft and minor axis, flitch around the equal fixedly connected with in both ends around the board upper end is even to L shape, flitch sliding connection is on two beads respectively around two, fixedly connected with articulated footstock between the upper end of flitch around two, connecting rod II's the other end articulates on articulated footstock, L shape is even the lower extreme fixedly connected with diaphragm of board, the upper end of main shaft is passed through the bearing frame and is rotated the connection on the diaphragm, the upper end fixedly connected with gear III of main shaft, the minor axis passes through the bearing frame and rotates the connection on the diaphragm, the upper end fixedly connected with gear II of minor axis, gear II and gear III meshing transmission, gear II's rear end and rack II meshing transmission, the lower extreme.
The resuscitation pond still includes the landing leg, and the landing leg is provided with four, and four landing legs are fixed connection respectively in four angle departments of the lower extreme of cell body, and the lower extreme of cell body is provided with the heating wire.
The resuscitation pond still includes fastening screw and fixed frame, the rear end fixedly connected with fixed frame of cell body, has fastening screw through threaded connection on the fixed frame.
The rocking frame further comprises a gear I, the left end and the right end of the rotating shaft are fixedly connected with the gear I, and the inner sides of the two gears I are attached to the outer sides of the two support plates.
Cell recovery device still includes the crane, the crane includes perpendicular traveller, rack I, the whippletree, the rack connecting rod, motor I, carousel I and connecting rod I, erect traveller sliding connection on fixed frame, fastening screw pushes up on erecting the traveller, the upper end fixedly connected with whippletree, the equal sliding connection in both ends has rack I about the whippletree, fixedly connected with rack connecting rod between two rack I's the rear end, the upper end fixedly connected with motor I of whippletree, motor I's fixedly connected with carousel I on having the section output shaft, connecting rod I's one end articulates at carousel I's eccentric position, connecting rod I's the other end articulates the upper end at rack connecting rod, two rack I respectively with two gear I's lower extreme meshing transmission.
The test-tube rack still includes board between disc, ring, test tube jack and connection, and the lower extreme fixed connection of main shaft is at the upper end center of disc, is the annular equipartition on the disc and has a plurality of test tube jacks, and the upper end fixed connection of board is on the disc between the connection, and the lower extreme fixed connection of board is on the ring between the connection, and the ring is located the lower extreme of a plurality of test tube jacks.
The main shaft, the disc and the circular ring are coaxial.
The cell recovery device has the beneficial effects that:
the invention provides a cell recovery device, which can put a plurality of test tubes filled with cell solution into warm water, can swing in the water in the front-back direction and the left-right direction, can swing in a reciprocating manner by taking the axis of a main shaft as an axis, accelerates the recovery of cells in the warm water, and can fully mix the cells in a culture solution.
Drawings
The invention is described in further detail below with reference to the accompanying drawings and specific embodiments.
FIG. 1 is a schematic diagram of the overall structure of a cell resuscitating device according to the present invention;
FIG. 2 is a schematic diagram of the overall structure of a cell resuscitating device according to the present invention;
FIG. 3 is a first schematic structural diagram of a resuscitation pool;
FIG. 4 is a schematic structural diagram II of a resuscitation pool;
FIG. 5 is a first structural schematic diagram of the lifting frame;
FIG. 6 is a structural schematic view II of the lifting frame;
FIG. 7 is a first schematic structural view of the rocking beam;
FIG. 8 is a second schematic structural view of the rocking beam;
FIG. 9 is a first schematic structural view of a test tube rack;
fig. 10 is a structural schematic diagram of a test tube rack.
In the figure: a resuscitation pond 1; 1-1 of a tank body; 1-2 of supporting legs; 1-3 of a support plate; 1-4 of a chute; fastening screws 1-5; a fixed frame 1-6; a lifting frame 2; 2-1 of vertical sliding column; rack I2-2; 2-3 of a horizontal bar; 2-4 of a rack connecting rod; motor I2-5; turntable I2-6; a connecting rod I2-7; a swing frame 3; gear I3-1; a rotating shaft 3-2; turntable II 3-3; motor II 3-4; 3-5 of a motor base; a connecting rod II 3-6; 3-7 parts of a middle rectangular block; 3-8 parts of convex ribs; connecting columns 3-9; rack II 3-10; a test tube rack 4; a transverse plate 4-1; gear II 4-2; gear III 4-3; 4-4 of front and rear flitch plates; 4-5 of a hinged top seat; 4-6 of L-shaped connecting plates; 4-7 of a main shaft; 4-8 of a disc; 4-9 parts of a circular ring; 4-10 parts of a test tube jack; the short shaft is 4-11; the intermediate plates 4-12 are connected.
Detailed Description
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, and are used only for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.
The first embodiment is as follows:
the present invention relates to the field of cells, and more particularly to a cell resuscitating device, which comprises a resuscitating pool 1, a lifting frame 2, a swinging frame 3 and a test tube rack 4, wherein a plurality of test tubes filled with cell solution can be placed in warm water, the test tubes can swing in the water in the front-back direction and the left-right direction, and the test tubes filled with cell solution can swing back and forth with the axis of a main shaft 4-7 as an axis, so that the resuscitating of cells in the warm water is accelerated, and the cells can be fully mixed in a culture solution.
The resuscitation pond 1 comprises a pond body 1-1, support plates 1-3 and sliding chutes 1-4, wherein the pond body 1-1 is in a rectangular box shape with an open upper end, the left end and the right end of the upper end of the pond body 1-1 are fixedly connected with the support plates 1-3, and the sliding chutes 1-4 are arranged on the two support plates 1-3; the tank body 1-1 is used for putting warm water so as to recover frozen cells.
The swing frame 3 comprises a rotating shaft 3-2, a rotary disc II3-3, a motor II3-4, a motor base 3-5, a connecting rod II3-6, a middle rectangular block 3-7, convex ribs 3-8, a connecting column 3-9 and a rack II3-10, wherein the middle end of the rotating shaft 3-2 is fixedly connected with the middle rectangular block 3-7, the front end and the rear end of the middle rectangular block 3-7 are fixedly connected with the convex ribs 3-8, the left end of the rotating shaft 3-2 is fixedly connected with the motor base 3-5, the upper end of the motor base 3-5 is fixedly connected with the motor II3-4, the front end output shaft of the motor II3-4 is fixedly connected with the rotary disc II3-3, the eccentric position of the rotary disc II3-3 is hinged with the connecting rod II3-6, and the right end of the rotating shaft 3-, the rack II3-10 is fixedly connected to the connecting column 3-9, and the left end and the right end of the rotating shaft 3-2 are respectively connected to the two sliding chutes 1-4 in a sliding manner; the left end and the right end of the rotating shaft 3-2 can rotate on the two sliding grooves 1-4 by self axes respectively, the left end and the right end of the rotating shaft 3-2 can also vertically slide on the two sliding grooves 1-4 respectively, and the output shaft of the motor II3-4 can drive the rotating disc II3-3 to rotate by taking the axis of the output shaft of the motor II3-4 as an axis when rotating.
The test tube rack 4 comprises a transverse plate 4-1, a gear II4-2, a gear III4-3, a front pasting plate 4-4, a rear pasting plate 4-4, a hinged top seat 4-5, an L-shaped connecting plate 4-6, a main shaft 4-7 and a short shaft 4-11, wherein the front end and the rear end of the upper end of the L-shaped connecting plate 4-6 are fixedly connected with the front pasting plate 4-4 and the rear pasting plate 4-4 respectively and slidably connected to two convex edges 3-8, the hinged top seat 4-5 is fixedly connected between the upper ends of the two front pasting plates 4-4 and the rear pasting plates 4-4, the other end of a connecting rod II3-6 is hinged to the hinged top seat 4-5, the lower end of the L-shaped connecting plate 4-6 is fixedly connected with the transverse plate 4-1, the upper end of the main shaft 4-7 is rotatably connected to the transverse plate 4, the short shaft 4-11 is rotatably connected to the transverse plate 4-1 through a bearing seat, the upper end of the short shaft 4-11 is fixedly connected with a gear II4-2, the gear II4-2 is in meshing transmission with a gear III4-3, the rear end of the gear II4-2 is in meshing transmission with a rack II3-10, and the lower end of the main shaft 4-7 is provided with a test tube placer. The two front and rear flitch plates 4-4 can slide left and right on the two convex edges 3-8 respectively, the rotary table II3-3 can drive the hinged footstock 4-5 and the two front and rear flitch plates 4-4 to reciprocate left and right when rotating by taking the axis of the output shaft of the motor II3-4 as a shaft, so that the whole test tube rack 4 can reciprocate left and right, when the transverse plate 4-1 reciprocates left and right, the gear II4-2 and the short shaft 4-11 can both rotate by taking the axis of the short shaft 4-11 as a shaft, and the gear II4-2 drives the gear III4-3, the main shaft 4-7 and the test tube placer to reciprocate forward and backward by taking the axis of the main shaft 4-7 as a shaft; the rotating shaft 3-2 can drive the whole test tube rack 4 to swing back and forth by taking the axis of the rotating shaft as the axis and rotate back and forth, the test tube placer is used for placing a plurality of test tubes, and the plurality of test tubes can swing back and forth, swing left and right and rotate forward and backward by taking the axis of the main shaft 4-7 as the axis. The recovery of the cells in warm water is accelerated, and the thawed cells can be fully mixed in a culture solution in a test tube.
The second embodiment is as follows:
the embodiment is described below with reference to fig. 1 to 10, and the embodiment further describes the first embodiment, the resuscitation pond 1 further includes four support legs 1-2, the four support legs 1-2 are respectively fixedly connected to four corners of the lower end of the pond body 1-1, and the lower end of the pond body 1-1 is provided with a heating wire. The supporting legs 1-2 play a supporting role, and the electric heating wires play a role in heating warm water in the tank body 1-1.
The third concrete implementation mode:
the embodiment is described below with reference to fig. 1 to 10, and the embodiment further describes the first embodiment, the resuscitation pool 1 further comprises fastening screws 1 to 5 and a fixing frame 1 to 6, the fixing frame 1 to 6 is fixedly connected to the rear end of the pool body 1 to 1, and the fastening screws 1 to 5 are connected to the fixing frame 1 to 6 through threads.
The fourth concrete implementation mode:
the third embodiment is further described with reference to fig. 1-10, the swing frame 3 further includes a gear I3-1, the left and right ends of the rotating shaft 3-2 are fixedly connected with a gear I3-1, and the inner sides of the two gears I3-1 are attached to the outer sides of the two brackets 1-3. The inner sides of the two gears I3-1 are attached to the outer sides of the two support plates 1-3, so that the rotating shaft 3-2 cannot move left and right between the two support plates 1-3.
The fifth concrete implementation mode:
the fourth embodiment is further described with reference to fig. 1-10, the cell resuscitating device further comprises a lifting frame 2, the lifting frame 2 comprises a vertical sliding column 2-1, a rack I2-2, a cross bar 2-3, a rack connecting rod 2-4, a motor I2-5, a turntable I2-6 and a connecting rod I2-7, the vertical sliding column 2-1 is slidably connected to a fixed frame 1-6, a fastening screw 1-5 is supported against the vertical sliding column 2-1, the upper end of the vertical sliding column 2-1 is fixedly connected with the cross bar 2-3, the left end and the right end of the cross bar 2-3 are both slidably connected with a rack I2-2, a rack connecting rod 2-4 is fixedly connected between the rear ends of the two racks I2-2, the upper end of the cross bar 2-3 is fixedly connected with a motor I2-5, a section output shaft of the motor I2-5 is fixedly connected with a turntable I2-6, one end of a connecting rod I2-7 is hinged at the eccentric position of the turntable I2-6, the other end of the connecting rod I2-7 is hinged at the upper end of a rack connecting rod 2-4, and two racks I2-2 are respectively in meshing transmission with the lower ends of two gears I3-1. The vertical sliding column 2-1 can vertically slide on the fixed frame 1-6, and the position of the vertical sliding column 2-1 can be fixed by rotating the fastening screw 1-5 to prop against the vertical sliding column 2-1. When the vertical sliding column 2-1 is lifted or lowered, the horizontal bar 2-3 and the two racks I2-2 are driven to be lifted or lowered, the two racks I2-2 respectively drive the two gears I3-1 and the whole swing frame 3 to be lifted or lowered, so that the test tube rack 4 is lifted or lowered along with the swing frame 3, and the depth of the test tubes immersed in warm water is adjusted according to the sizes of the test tubes. When the output shaft of the motor I2-5 rotates, the rotary table I2-6 is driven to rotate by taking the axis of the output shaft of the motor I2-5 as a shaft, the rotary table I2-6 drives the rack connecting rod 2-4 to reciprocate back and forth through the connecting rod I2-7, the rack connecting rod 2-4 drives the two racks I2-2 to reciprocate back and forth, and the two racks I2-2 respectively drive the two gears I3-1 to reciprocate back and forth when reciprocating back and forth, so that the rotary shaft 3-2 is driven to rotate back and forth by taking the axis of the rotary shaft as a shaft.
The sixth specific implementation mode:
the following describes the present embodiment with reference to fig. 1 to 10, which further describes the first embodiment, the test tube rack 4 further includes a disk 4 to 8, a ring 4 to 9, test tube jacks 4 to 10, and a connection intermediate plate 4 to 12, the lower end of the main shaft 4 to 7 is fixedly connected to the center of the upper end of the disk 4 to 8, a plurality of test tube jacks 4 to 10 are annularly and uniformly distributed on the disk 4 to 8, the upper end of the connection intermediate plate 4 to 12 is fixedly connected to the disk 4 to 8, the lower end of the connection intermediate plate 4 to 12 is fixedly connected to the ring 4 to 9, and the ring 4 to 9 is located at the lower end of the plurality of test tube jacks 4 to 10. The test tube inserting holes 4-10 are used for inserting test tubes, the bottoms of the test tubes are supported on the circular rings 4-9, and the test tubes cannot slide off the inserting holes 4-10.
The seventh embodiment:
the present embodiment is described below with reference to fig. 1 to 10, and the present embodiment further describes the first embodiment, in which the main shafts 4 to 7, the disks 4 to 8, and the rings 4 to 9 are coaxial.
The working principle of the invention is as follows: the tank body 1-1 is used for putting warm water so as to recover frozen cells. The left end and the right end of the rotating shaft 3-2 can rotate on the two sliding grooves 1-4 by self axes respectively, the left end and the right end of the rotating shaft 3-2 can also vertically slide on the two sliding grooves 1-4 respectively, and the output shaft of the motor II3-4 can drive the rotating disc II3-3 to rotate by taking the axis of the output shaft of the motor II3-4 as an axis when rotating. The two front and rear flitch plates 4-4 can slide left and right on the two convex edges 3-8 respectively, the rotary table II3-3 can drive the hinged footstock 4-5 and the two front and rear flitch plates 4-4 to reciprocate left and right when rotating by taking the axis of the output shaft of the motor II3-4 as a shaft, so that the whole test tube rack 4 can reciprocate left and right, when the transverse plate 4-1 reciprocates left and right, the gear II4-2 and the short shaft 4-11 can both rotate by taking the axis of the short shaft 4-11 as a shaft, and the gear II4-2 drives the gear III4-3, the main shaft 4-7 and the test tube placer to reciprocate forward and backward by taking the axis of the main shaft 4-7 as a shaft; the rotating shaft 3-2 can drive the whole test tube rack 4 to swing back and forth by taking the axis of the rotating shaft as the axis and rotate back and forth, the test tube placer is used for placing a plurality of test tubes, and the plurality of test tubes can swing back and forth, swing left and right and rotate forward and backward by taking the axis of the main shaft 4-7 as the axis. The recovery of the cells in warm water is accelerated, and the thawed cells can be fully mixed in a culture solution in a test tube. The supporting legs 1-2 play a supporting role, and the electric heating wires play a role in heating warm water in the tank body 1-1. The inner sides of the two gears I3-1 are attached to the outer sides of the two support plates 1-3, so that the rotating shaft 3-2 cannot move left and right between the two support plates 1-3. The vertical sliding column 2-1 can vertically slide on the fixed frame 1-6, and the position of the vertical sliding column 2-1 can be fixed by rotating the fastening screw 1-5 to prop against the vertical sliding column 2-1. When the vertical sliding column 2-1 is lifted or lowered, the horizontal bar 2-3 and the two racks I2-2 are driven to be lifted or lowered, the two racks I2-2 respectively drive the two gears I3-1 and the whole swing frame 3 to be lifted or lowered, so that the test tube rack 4 is lifted or lowered along with the swing frame 3, and the depth of the test tubes immersed in warm water is adjusted according to the sizes of the test tubes. When the output shaft of the motor I2-5 rotates, the rotary table I2-6 is driven to rotate by taking the axis of the output shaft of the motor I2-5 as a shaft, the rotary table I2-6 drives the rack connecting rod 2-4 to reciprocate back and forth through the connecting rod I2-7, the rack connecting rod 2-4 drives the two racks I2-2 to reciprocate back and forth, and the two racks I2-2 respectively drive the two gears I3-1 to reciprocate back and forth when reciprocating back and forth, so that the rotary shaft 3-2 is driven to rotate back and forth by taking the axis of the rotary shaft as a shaft. The test tube inserting holes 4-10 are used for inserting test tubes, the bottoms of the test tubes are supported on the circular rings 4-9, and the test tubes cannot slide off the inserting holes 4-10.
It is to be understood that the above description is not intended to limit the present invention, and the present invention is not limited to the above examples, and that various changes, modifications, additions and substitutions which are within the spirit and scope of the present invention and which may be made by those skilled in the art are also within the scope of the present invention.
Claims (2)
1. The utility model provides a cell recovery device, includes recovery pond (1), rocking beam (3) and test-tube rack (4), its characterized in that: the resuscitation pond (1) comprises a pond body (1-1), support plates (1-3) and sliding chutes (1-4), wherein the pond body (1-1) is in a rectangular box shape with an open upper end, the support plates (1-3) are fixedly connected to the left end and the right end of the upper end of the pond body (1-1), and the sliding chutes (1-4) are arranged on the two support plates (1-3);
the swing frame (3) comprises a rotating shaft (3-2), a rotary disc II (3-3), a motor II (3-4), a motor base (3-5), a connecting rod II (3-6), a middle rectangular block (3-7), ribs (3-8), connecting columns (3-9) and racks II (3-10), the middle end of the rotating shaft (3-2) is fixedly connected with the middle rectangular block (3-7), the front end and the rear end of the middle rectangular block (3-7) are fixedly connected with the ribs (3-8), the left end of the rotating shaft (3-2) is fixedly connected with the motor base (3-5), the upper end of the motor base (3-5) is fixedly connected with the motor II (3-4), and the front end output shaft of the motor II (3-4) is fixedly connected with the rotary disc II (3-3), the eccentric position of the rotary table II (3-3) is hinged with a connecting rod II (3-6), the right end of the rotary shaft (3-2) is fixedly connected with a connecting column (3-9), a rack II (3-10) is fixedly connected to the connecting column (3-9), and the left end and the right end of the rotary shaft (3-2) are respectively connected to the two sliding chutes (1-4) in a sliding manner;
the test tube rack (4) comprises a transverse plate (4-1), a gear II (4-2), a gear III (4-3), front and rear flitch plates (4-4), a hinged footstock (4-5), an L-shaped connecting plate (4-6), a main shaft (4-7) and a short shaft (4-11), wherein the front and rear ends of the upper end of the L-shaped connecting plate (4-6) are fixedly connected with the front and rear flitch plates (4-4), the two front and rear flitch plates (4-4) are respectively connected on the two convex edges (3-8) in a sliding manner, the hinged footstock (4-5) is fixedly connected between the upper ends of the two front and rear flitch plates (4-4), the other end of a connecting rod II (3-6) is hinged on the hinged footstock (4-5), and the lower end of the L-shaped connecting plate (4-6) is fixedly, the upper end of a main shaft (4-7) is rotatably connected to a transverse plate (4-1) through a bearing seat, the upper end of the main shaft (4-7) is fixedly connected with a gear III (4-3), a short shaft (4-11) is rotatably connected to the transverse plate (4-1) through the bearing seat, the upper end of the short shaft (4-11) is fixedly connected with a gear II (4-2), the gear II (4-2) is in meshing transmission with the gear III (4-3), the rear end of the gear II (4-2) is in meshing transmission with a rack II (3-10), and the lower end of the main shaft (4-7) is provided with a test tube placer;
the resuscitation pond (1) further comprises four support legs (1-2), the four support legs (1-2) are respectively fixedly connected to four corners of the lower end of the pond body (1-1), and the lower end of the pond body (1-1) is provided with an electric heating wire;
the swing frame (3) further comprises gears I (3-1), the left end and the right end of the rotating shaft (3-2) are fixedly connected with the gears I (3-1), and the inner sides of the two gears I (3-1) are attached to the outer sides of the two support plates (1-3);
the cell resuscitation device also comprises a lifting frame (2), the lifting frame (2) comprises vertical sliding columns (2-1), racks I (2-2), cross bars (2-3), rack connecting rods (2-4), motors I (2-5), rotary discs I (2-6) and connecting rods I (2-7), the vertical sliding columns (2-1) are connected to the fixing frames (1-6) in a sliding mode, fastening screws (1-5) are supported on the vertical sliding columns (2-1), the upper ends of the vertical sliding columns (2-1) are fixedly connected with the cross bars (2-3), the left end and the right end of each cross bar (2-3) are connected with the racks I (2-2) in a sliding mode, the rack connecting rods (2-4) are fixedly connected between the rear ends of the two racks I (2-2), the upper ends of the cross bars (2-3) are fixedly connected with the motors I (2-5), a section output shaft of the motor I (2-5) is fixedly connected with a turntable I (2-6), one end of a connecting rod I (2-7) is hinged at the eccentric position of the turntable I (2-6), the other end of the connecting rod I (2-7) is hinged at the upper end of a rack connecting rod (2-4), and two racks I (2-2) are respectively in meshing transmission with the lower ends of two gears I (3-1);
the test tube rack (4) also comprises a disc (4-8), a ring (4-9), test tube jacks (4-10) and a connecting intermediate plate (4-12), the lower end of the main shaft (4-7) is fixedly connected to the center of the upper end of the disc (4-8), a plurality of test tube jacks (4-10) are uniformly distributed on the disc (4-8) in an annular manner, the upper end of the connecting intermediate plate (4-12) is fixedly connected to the disc (4-8), the lower end of the connecting intermediate plate (4-12) is fixedly connected to the ring (4-9), the ring (4-9) is positioned at the lower end of the plurality of test tube jacks (4-10), and the disc (4-8), the ring (4-9), the test tube jacks (4-10) and the connecting intermediate plate (4-12) form the test tube placer.
2. A cell resuscitation device according to claim 1, wherein: the main shaft (4-7), the disc (4-8) and the circular ring (4-9) are coaxial.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910867559.7A CN110591900B (en) | 2018-11-22 | 2018-11-22 | Cell recovery device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910867559.7A CN110591900B (en) | 2018-11-22 | 2018-11-22 | Cell recovery device |
| CN201811399432.9A CN109401917B (en) | 2018-11-22 | 2018-11-22 | A cell resuscitation device |
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| CN201811399432.9A Division CN109401917B (en) | 2018-11-22 | 2018-11-22 | A cell resuscitation device |
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| CN110591900B CN110591900B (en) | 2022-10-25 |
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| CN201811399432.9A Active CN109401917B (en) | 2018-11-22 | 2018-11-22 | A cell resuscitation device |
| CN201910867560.XA Active CN110724624B (en) | 2018-11-22 | 2018-11-22 | Cell recovery device |
| CN201910867559.7A Active CN110591900B (en) | 2018-11-22 | 2018-11-22 | Cell recovery device |
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| CN201811399432.9A Active CN109401917B (en) | 2018-11-22 | 2018-11-22 | A cell resuscitation device |
| CN201910867560.XA Active CN110724624B (en) | 2018-11-22 | 2018-11-22 | Cell recovery device |
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Cited By (1)
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| CN116555029A (en) * | 2023-05-16 | 2023-08-08 | 宁波宇泰医疗器械有限公司 | Stem cell culture system with high survival rate |
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| CN110157599A (en) * | 2019-07-10 | 2019-08-23 | 陕西中医药大学 | A large-scale culture equipment for culturing umbilical cord mesenchymal stem cells |
| CN112899129A (en) * | 2021-01-15 | 2021-06-04 | 吴应迪 | Cell resuscitator with lifting function |
| CN112961775B (en) * | 2021-03-10 | 2023-05-12 | 秦娟 | Cell resuscitator |
| CN113073033A (en) * | 2021-03-23 | 2021-07-06 | 曾小广 | Cell rapid recovery device for medical clinical laboratory |
| CN113999771B (en) * | 2021-12-31 | 2022-04-08 | 迈赛凯尔(山东)生命科学有限公司 | Immature dendritic cell recovery device |
| CN117965305B (en) * | 2024-03-25 | 2025-07-01 | 西北师范大学 | A cell transfection device |
| CN120424746A (en) * | 2025-05-09 | 2025-08-05 | 南京三生生物技术股份有限公司 | A cell resuscitation device |
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Also Published As
| Publication number | Publication date |
|---|---|
| CN110724624A (en) | 2020-01-24 |
| CN110591900B (en) | 2022-10-25 |
| CN109401917B (en) | 2019-10-29 |
| CN109401917A (en) | 2019-03-01 |
| CN110724624B (en) | 2022-11-11 |
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