CN110571005B - 一种固定化金属离子-磁性脂质体及其制备方法和应用 - Google Patents
一种固定化金属离子-磁性脂质体及其制备方法和应用 Download PDFInfo
- Publication number
- CN110571005B CN110571005B CN201910923026.6A CN201910923026A CN110571005B CN 110571005 B CN110571005 B CN 110571005B CN 201910923026 A CN201910923026 A CN 201910923026A CN 110571005 B CN110571005 B CN 110571005B
- Authority
- CN
- China
- Prior art keywords
- magnetic
- liposome
- metal ion
- magnetic liposome
- immobilized metal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000002502 liposome Substances 0.000 title claims abstract description 121
- 229910052751 metal Inorganic materials 0.000 title claims abstract description 51
- 239000002184 metal Substances 0.000 title claims abstract description 51
- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- 238000003756 stirring Methods 0.000 claims abstract description 21
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 19
- 150000003904 phospholipids Chemical class 0.000 claims abstract description 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N EtOH Substances CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 13
- 229910001428 transition metal ion Inorganic materials 0.000 claims abstract description 13
- 229910021577 Iron(II) chloride Inorganic materials 0.000 claims abstract description 12
- 238000006243 chemical reaction Methods 0.000 claims abstract description 12
- NMCUIPGRVMDVDB-UHFFFAOYSA-L iron dichloride Chemical compound Cl[Fe]Cl NMCUIPGRVMDVDB-UHFFFAOYSA-L 0.000 claims abstract description 12
- 230000008878 coupling Effects 0.000 claims abstract description 5
- 238000010168 coupling process Methods 0.000 claims abstract description 5
- 238000005859 coupling reaction Methods 0.000 claims abstract description 5
- 239000012528 membrane Substances 0.000 claims abstract description 5
- SZVJSHCCFOBDDC-UHFFFAOYSA-N ferrosoferric oxide Chemical compound O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 claims abstract description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 54
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 54
- 239000008367 deionised water Substances 0.000 claims description 36
- 229910021641 deionized water Inorganic materials 0.000 claims description 36
- 239000002245 particle Substances 0.000 claims description 30
- 230000009471 action Effects 0.000 claims description 29
- 229910001868 water Inorganic materials 0.000 claims description 18
- 238000000926 separation method Methods 0.000 claims description 13
- 238000005406 washing Methods 0.000 claims description 13
- 125000000217 alkyl group Chemical group 0.000 claims description 9
- 229910000363 nickel(II) sulfate Inorganic materials 0.000 claims description 9
- 229910052757 nitrogen Inorganic materials 0.000 claims description 9
- 102000004169 proteins and genes Human genes 0.000 claims description 8
- 108090000623 proteins and genes Proteins 0.000 claims description 7
- 238000005303 weighing Methods 0.000 claims description 4
- NRJAVPSFFCBXDT-HUESYALOSA-N 1,2-distearoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCCCCCCCCCCC NRJAVPSFFCBXDT-HUESYALOSA-N 0.000 claims description 2
- LVNGJLRDBYCPGB-UHFFFAOYSA-N 1,2-distearoylphosphatidylethanolamine Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(COP([O-])(=O)OCC[NH3+])OC(=O)CCCCCCCCCCCCCCCCC LVNGJLRDBYCPGB-UHFFFAOYSA-N 0.000 claims description 2
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 claims description 2
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 claims description 2
- JZNWSCPGTDBMEW-UHFFFAOYSA-N Glycerophosphorylethanolamin Natural products NCCOP(O)(=O)OCC(O)CO JZNWSCPGTDBMEW-UHFFFAOYSA-N 0.000 claims description 2
- MWRBNPKJOOWZPW-CLFAGFIQSA-N dioleoyl phosphatidylethanolamine Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OCC(COP(O)(=O)OCCN)OC(=O)CCCCCCC\C=C/CCCCCCCC MWRBNPKJOOWZPW-CLFAGFIQSA-N 0.000 claims description 2
- 238000010438 heat treatment Methods 0.000 claims description 2
- 229940067606 lecithin Drugs 0.000 claims description 2
- 235000010445 lecithin Nutrition 0.000 claims description 2
- 239000000787 lecithin Substances 0.000 claims description 2
- 238000010907 mechanical stirring Methods 0.000 claims description 2
- -1 palmitoyl oleoyl phosphatidylethanolamine Chemical class 0.000 claims description 2
- WTJKGGKOPKCXLL-RRHRGVEJSA-N phosphatidylcholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCC=CCCCCCCCC WTJKGGKOPKCXLL-RRHRGVEJSA-N 0.000 claims description 2
- 239000008347 soybean phospholipid Substances 0.000 claims description 2
- 229910021645 metal ion Inorganic materials 0.000 abstract description 6
- 238000001179 sorption measurement Methods 0.000 abstract description 6
- 238000000034 method Methods 0.000 abstract description 5
- 229910021578 Iron(III) chloride Inorganic materials 0.000 abstract description 3
- 210000000170 cell membrane Anatomy 0.000 abstract description 3
- 230000009977 dual effect Effects 0.000 abstract description 3
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 abstract description 3
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 abstract description 2
- 235000011114 ammonium hydroxide Nutrition 0.000 abstract description 2
- 238000011065 in-situ storage Methods 0.000 abstract description 2
- AFPRBBQKUYJMJD-UHFFFAOYSA-N 2-[carboxymethyl(hexadecyl)amino]acetic acid Chemical compound CCCCCCCCCCCCCCCCN(CC(O)=O)CC(O)=O AFPRBBQKUYJMJD-UHFFFAOYSA-N 0.000 abstract 1
- 229910001873 dinitrogen Inorganic materials 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 38
- 239000000203 mixture Substances 0.000 description 18
- 238000005516 engineering process Methods 0.000 description 8
- 239000002253 acid Substances 0.000 description 7
- LGQLOGILCSXPEA-UHFFFAOYSA-L nickel sulfate Chemical compound [Ni+2].[O-]S([O-])(=O)=O LGQLOGILCSXPEA-UHFFFAOYSA-L 0.000 description 7
- 229910000069 nitrogen hydride Inorganic materials 0.000 description 6
- 238000007885 magnetic separation Methods 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000003760 magnetic stirring Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 102000018832 Cytochromes Human genes 0.000 description 1
- 108010052832 Cytochromes Proteins 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 108091005461 Nucleic proteins Proteins 0.000 description 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
- 239000003012 bilayer membrane Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000000975 co-precipitation Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000026058 directional locomotion Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000635 electron micrograph Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000009775 high-speed stirring Methods 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 239000006249 magnetic particle Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 108091005981 phosphorylated proteins Proteins 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000013341 scale-up Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3804—Affinity chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01F—MAGNETS; INDUCTANCES; TRANSFORMERS; SELECTION OF MATERIALS FOR THEIR MAGNETIC PROPERTIES
- H01F1/00—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties
- H01F1/0036—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties showing low dimensional magnetism, i.e. spin rearrangements due to a restriction of dimensions, e.g. showing giant magnetoresistivity
- H01F1/0045—Zero dimensional, e.g. nanoparticles, soft nanoparticles for medical/biological use
- H01F1/0054—Coated nanoparticles, e.g. nanoparticles coated with organic surfactant
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01F—MAGNETS; INDUCTANCES; TRANSFORMERS; SELECTION OF MATERIALS FOR THEIR MAGNETIC PROPERTIES
- H01F41/00—Apparatus or processes specially adapted for manufacturing or assembling magnets, inductances or transformers; Apparatus or processes specially adapted for manufacturing materials characterised by their magnetic properties
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Power Engineering (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Nanotechnology (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Manufacturing & Machinery (AREA)
- Inorganic Chemistry (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Medicinal Preparation (AREA)
- Manufacturing Of Micro-Capsules (AREA)
Abstract
Description
Claims (5)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910923026.6A CN110571005B (zh) | 2019-09-27 | 2019-09-27 | 一种固定化金属离子-磁性脂质体及其制备方法和应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910923026.6A CN110571005B (zh) | 2019-09-27 | 2019-09-27 | 一种固定化金属离子-磁性脂质体及其制备方法和应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN110571005A CN110571005A (zh) | 2019-12-13 |
CN110571005B true CN110571005B (zh) | 2021-01-01 |
Family
ID=68782758
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201910923026.6A Active CN110571005B (zh) | 2019-09-27 | 2019-09-27 | 一种固定化金属离子-磁性脂质体及其制备方法和应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN110571005B (zh) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111362427B (zh) * | 2020-04-17 | 2021-06-04 | 华禹源环保科技(淄博)有限公司 | 一种载微生物重金属污水处理3d打印材料及其制备方法和应用 |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101564380A (zh) * | 2009-05-26 | 2009-10-28 | 西北工业大学 | 磁性脂质体及其制备方法 |
CN102000351A (zh) * | 2010-12-02 | 2011-04-06 | 上海纳米技术及应用国家工程研究中心有限公司 | 壳聚糖修饰的磁性脂质体的制备方法 |
CN103126990A (zh) * | 2011-11-23 | 2013-06-05 | 苏州苏大赛尔免疫生物技术有限公司 | 一种靶向磁性载药脂质体的制备方法 |
CN104023793A (zh) * | 2011-10-31 | 2014-09-03 | 马林克罗特有限公司 | 用于治疗癌症的联合脂质体组合物 |
CN105188675A (zh) * | 2013-03-13 | 2015-12-23 | 马林克罗特有限公司 | 经修饰多西他赛脂质体制剂 |
CN105434355A (zh) * | 2015-12-09 | 2016-03-30 | 燕山大学 | 一种Fe3O4磁性尼莫地平脂质体及其制备方法 |
CN108033892A (zh) * | 2017-12-27 | 2018-05-15 | 广西科技大学 | 一种n-烷基亚氨基二乙酸的制备方法 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20090297626A1 (en) * | 2006-11-03 | 2009-12-03 | The Trustees Of Columbia University In The City Of New York | Methods for preparing metal oxides |
-
2019
- 2019-09-27 CN CN201910923026.6A patent/CN110571005B/zh active Active
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101564380A (zh) * | 2009-05-26 | 2009-10-28 | 西北工业大学 | 磁性脂质体及其制备方法 |
CN102000351A (zh) * | 2010-12-02 | 2011-04-06 | 上海纳米技术及应用国家工程研究中心有限公司 | 壳聚糖修饰的磁性脂质体的制备方法 |
CN104023793A (zh) * | 2011-10-31 | 2014-09-03 | 马林克罗特有限公司 | 用于治疗癌症的联合脂质体组合物 |
CN103126990A (zh) * | 2011-11-23 | 2013-06-05 | 苏州苏大赛尔免疫生物技术有限公司 | 一种靶向磁性载药脂质体的制备方法 |
CN105188675A (zh) * | 2013-03-13 | 2015-12-23 | 马林克罗特有限公司 | 经修饰多西他赛脂质体制剂 |
CN105434355A (zh) * | 2015-12-09 | 2016-03-30 | 燕山大学 | 一种Fe3O4磁性尼莫地平脂质体及其制备方法 |
CN108033892A (zh) * | 2017-12-27 | 2018-05-15 | 广西科技大学 | 一种n-烷基亚氨基二乙酸的制备方法 |
Non-Patent Citations (1)
Title |
---|
Fe3O4磁性亚微球金属螯合亲和介质的制备及应用研究;高华峰;《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》;20180415;正文第一、二章 * |
Also Published As
Publication number | Publication date |
---|---|
CN110571005A (zh) | 2019-12-13 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Wang et al. | Magnetic nanoparticles grafted with amino-riched dendrimer as magnetic flocculant for efficient harvesting of oleaginous microalgae | |
Cheng et al. | The GO/rGO–Fe 3 O 4 composites with good water-dispersibility and fast magnetic response for effective immobilization and enrichment of biomolecules | |
US6355166B1 (en) | Magnetically enhanced composite materials and methods for making and using the same | |
Zhao et al. | Gold atom-decorated CoSe 2 nanobelts with engineered active sites for enhanced oxygen evolution | |
CN105771908B (zh) | 一种用于重金属吸附的磁性二氧化硅核壳复合材料及其制备方法 | |
CN102280634B (zh) | 一种多孔结构锰酸锂电极材料和制备方法及应用 | |
CN110571005B (zh) | 一种固定化金属离子-磁性脂质体及其制备方法和应用 | |
CN113999399B (zh) | 一种双功能化mof材料及其制备和应用 | |
CN102838984A (zh) | 一种胰凝乳蛋白酶保护金纳米团簇荧光材料的制备方法 | |
CN107140644A (zh) | 一种室温具有流体行为的SiO2多孔液体及制备方法 | |
Wang et al. | Space-confined pyrolysis for fabrication of peacods-like Fe3O4@ C-Ni nanostructures for catalysis and protein adsorption | |
CN102174195B (zh) | 一种核-壳式磁性导电聚合物微球的制备方法及其应用 | |
Chen et al. | EDTA-directed self-assembly and enhanced catalytic properties of sphere-constructed platinum nanochains | |
CN114045522A (zh) | NiMo6-S@HCS纳米复合材料、制备方法及电催化制氢中的应用 | |
CN107417588B (zh) | 一种新型离子液体及采用其电解制备纳米铝的方法 | |
Oleinikova et al. | Self‐Association Based on Interfacial Structured Water Leads to {Mo154}≈ 1165 Super Clusters: A Dielectric Study | |
CN108043371A (zh) | 一种双模板导向印迹复合膜的制备方法及其应用 | |
CN110702759A (zh) | 一种检测甲胎蛋白的zif-8复合材料电化学免疫传感器及其制备方法和应用 | |
CN110189921A (zh) | 一种镍钴氧化物/氮掺杂石墨烯复合材料的制备方法 | |
CN107342173B (zh) | 一种柔性超级电容器电极及其制备方法 | |
Shen et al. | Upcycling of spent NiMH battery material—reconditioned battery alloys show faster activation and reaction kinetics than pristine alloys | |
CN111690149B (zh) | 一种24核有机磷铜团簇有机纳米框架封装多金属氧酸盐材料及其制备方法和应用 | |
CN111551619B (zh) | 一种磺氨生物素修饰氮化铟糊电极传感器的制备方法 | |
CN107256950A (zh) | 磁性金属磷化物/石墨烯纳米复合材料的制备方法、锂离子电池负极、锂离子电池 | |
CN107603591B (zh) | 一种荧光微胶囊及其制备方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20240116 Address after: 710000 Factory Building 5, South Zone 1, Hongshengxing Aviation Technology Industrial Park, Dunhua Road, Airport New City, Xixian New District, Xi'an City, Shaanxi Province Patentee after: Xi'an Meinan Biotechnology Co.,Ltd. Address before: No. 99 Xinfeng Avenue, Jinfeng Town, Chongqing High tech Zone, Jiulongpo District, Chongqing, 404100 Patentee before: Chongqing Science City Intellectual Property Operation Center Co.,Ltd. Effective date of registration: 20240116 Address after: No. 99 Xinfeng Avenue, Jinfeng Town, Chongqing High tech Zone, Jiulongpo District, Chongqing, 404100 Patentee after: Chongqing Science City Intellectual Property Operation Center Co.,Ltd. Address before: 545006 Guangxi University of science and technology, 268 Donghuan Avenue, Liuzhou City, Guangxi Zhuang Autonomous Region Patentee before: GUANGXI University OF SCIENCE AND TECHNOLOGY |
|
TR01 | Transfer of patent right |