CN110564742B - 一种yebN基因改造的重组菌株及其构建方法与应用 - Google Patents
一种yebN基因改造的重组菌株及其构建方法与应用 Download PDFInfo
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- CN110564742B CN110564742B CN201910804688.1A CN201910804688A CN110564742B CN 110564742 B CN110564742 B CN 110564742B CN 201910804688 A CN201910804688 A CN 201910804688A CN 110564742 B CN110564742 B CN 110564742B
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- strain
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
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- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
成分 | 配方g/L |
葡萄糖 | 40 |
硫酸铵 | 12 |
磷酸二氢钾 | 0.8 |
七水硫酸镁 | 0.8 |
七水硫酸亚铁 | 0.01 |
一水硫酸锰 | 0.01 |
酵母提取物 | 1.5 |
碳酸钙 | 0.5 |
L-甲硫氨酸 | 0.5 |
氢氧化钾调节pH值 | pH 7.0 |
Claims (10)
Priority Applications (8)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910804688.1A CN110564742B (zh) | 2019-08-28 | 2019-08-28 | 一种yebN基因改造的重组菌株及其构建方法与应用 |
KR1020227005071A KR20220034218A (ko) | 2019-08-28 | 2020-08-27 | 대장균 기반 재조합 균주 및 이의 구축 방법과 응용 |
BR112022002535A BR112022002535A2 (pt) | 2019-08-28 | 2020-08-27 | Sequência nucleotídica, proteína recombinante, vetor recombinante, cepa recombinante, método de construção para a cepa recombinante, e, uso de uma sequência nucleotídica, de uma proteína recombinante, de um vetor recombinante ou de uma cepa recombinante |
EP20859580.1A EP3992293A4 (en) | 2019-08-28 | 2020-08-27 | RECOMBINANT ESCHERICHIA COLI-BASED STRAIN, PROCESS FOR ITS PRODUCTION AND ITS USE |
PCT/CN2020/111842 WO2021037166A1 (zh) | 2019-08-28 | 2020-08-27 | 基于大肠杆菌的重组菌株及其构建方法与应用 |
US17/753,368 US20220324919A1 (en) | 2019-08-28 | 2020-08-27 | Escherichia coli-based recombinant strain, construction method therefor and use thereof |
EP23180518.5A EP4253569A3 (en) | 2019-08-28 | 2020-08-27 | Escherichia coli-based recombinant strain, construction method therefor and use thereof |
JP2022513939A JP7471395B2 (ja) | 2019-08-28 | 2020-08-27 | 大腸菌に基づく組換え株ならびにその構築方法および使用 |
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CN201910804688.1A CN110564742B (zh) | 2019-08-28 | 2019-08-28 | 一种yebN基因改造的重组菌株及其构建方法与应用 |
Publications (2)
Publication Number | Publication Date |
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CN110564742A CN110564742A (zh) | 2019-12-13 |
CN110564742B true CN110564742B (zh) | 2020-08-21 |
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CN201910804688.1A Active CN110564742B (zh) | 2019-08-28 | 2019-08-28 | 一种yebN基因改造的重组菌株及其构建方法与应用 |
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CN (1) | CN110564742B (zh) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
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EP3992293A4 (en) * | 2019-08-28 | 2023-01-04 | Heilongjiang Eppen Biotech Co., Ltd. | RECOMBINANT ESCHERICHIA COLI-BASED STRAIN, PROCESS FOR ITS PRODUCTION AND ITS USE |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN108998462B (zh) * | 2018-08-01 | 2021-06-18 | 武汉康复得生物科技股份有限公司 | 含锰离子重组蛋白的大肠杆菌表达系统及其应用方法 |
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2019
- 2019-08-28 CN CN201910804688.1A patent/CN110564742B/zh active Active
Non-Patent Citations (1)
Title |
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"GenBank:CP011323.1";匿名;《GenBank》;20151222;第1页、第9页、第17页CDS和translation部分 * |
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GR01 | Patent grant | ||
GR01 | Patent grant | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A yebn gene modified recombinant strain and its construction method and Application Effective date of registration: 20210715 Granted publication date: 20200821 Pledgee: Heilongjiang Xinzheng financing guarantee Group Co.,Ltd. Pledgor: Heilongjiang Yipin Biotechnology Co.,Ltd. Registration number: Y2021230000019 |
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PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20220926 Granted publication date: 20200821 Pledgee: Heilongjiang Xinzheng financing guarantee Group Co.,Ltd. Pledgor: Heilongjiang Yipin Biotechnology Co.,Ltd. Registration number: Y2021230000019 |
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PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A YebN Gene Modified Recombinant Strain and Its Construction Method and Application Effective date of registration: 20220927 Granted publication date: 20200821 Pledgee: Heilongjiang Xinzheng financing guarantee Group Co.,Ltd. Pledgor: Heilongjiang Yipin Biotechnology Co.,Ltd. Registration number: Y2022230000071 |