BR112022002535A2 - Sequência nucleotídica, proteína recombinante, vetor recombinante, cepa recombinante, método de construção para a cepa recombinante, e, uso de uma sequência nucleotídica, de uma proteína recombinante, de um vetor recombinante ou de uma cepa recombinante - Google Patents

Sequência nucleotídica, proteína recombinante, vetor recombinante, cepa recombinante, método de construção para a cepa recombinante, e, uso de uma sequência nucleotídica, de uma proteína recombinante, de um vetor recombinante ou de uma cepa recombinante

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Publication number
BR112022002535A2
BR112022002535A2 BR112022002535A BR112022002535A BR112022002535A2 BR 112022002535 A2 BR112022002535 A2 BR 112022002535A2 BR 112022002535 A BR112022002535 A BR 112022002535A BR 112022002535 A BR112022002535 A BR 112022002535A BR 112022002535 A2 BR112022002535 A2 BR 112022002535A2
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BR
Brazil
Prior art keywords
recombinant
strain
nucleotide sequence
vector
protein
Prior art date
Application number
BR112022002535A
Other languages
English (en)
Inventor
Aiying Wei
Chunguang Zhao
Fengyong Ma
Gang Meng
Houbo Su
Huiping Jia
Lipeng Yang
Xiaohang Gao
Xiaoqun Zhou
Original Assignee
Heilongjiang Eppen Biotech Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CN201910804688.1A external-priority patent/CN110564742B/zh
Priority claimed from CN201910804679.2A external-priority patent/CN110592109B/zh
Priority claimed from CN201910926295.8A external-priority patent/CN110804617B/zh
Application filed by Heilongjiang Eppen Biotech Co Ltd filed Critical Heilongjiang Eppen Biotech Co Ltd
Publication of BR112022002535A2 publication Critical patent/BR112022002535A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1235—Diphosphotransferases (2.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C12Y204/99012—Lipid IVA 3-deoxy-D-manno-octulosonic acid transferase (2.4.99.12)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/06—Diphosphotransferases (2.7.6)
    • C12Y207/06005—GTP diphosphokinase (2.7.6.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/07—Diphosphoric monoester hydrolases (3.1.7)
    • C12Y301/07002—Guanosine-3',5'-bis(diphosphate) 3'-diphosphatase (3.1.7.2)

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

sequência nucleotídica, proteína recombinante, vetor recombinante, cepa recombinante, método de construção para a cepa recombinante, e, uso de uma sequência nucleotídica, de uma proteína recombinante, de um vetor recombinante ou de uma cepa recombinante. são providas uma cepa recombinante baseada na cepa k12 de escherichia coli ou uma cepa derivada da mesma após mutagênese dirigida, um método de construção para as mesmas e uso das mesmas. a dita cepa recombinante é submetida a mutagênese dirigida contra o gene kdta, gene spot ou gene yebn, e pode produzir maior concentração de l-treonina em comparação com uma cepa de tipo selvagem não mutada.
BR112022002535A 2019-08-28 2020-08-27 Sequência nucleotídica, proteína recombinante, vetor recombinante, cepa recombinante, método de construção para a cepa recombinante, e, uso de uma sequência nucleotídica, de uma proteína recombinante, de um vetor recombinante ou de uma cepa recombinante BR112022002535A2 (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CN201910804688.1A CN110564742B (zh) 2019-08-28 2019-08-28 一种yebN基因改造的重组菌株及其构建方法与应用
CN201910804679.2A CN110592109B (zh) 2019-08-28 2019-08-28 一种spoT基因改造的重组菌株及其构建方法与应用
CN201910926295.8A CN110804617B (zh) 2019-09-27 2019-09-27 一种kdtA基因改造的重组菌株及其构建方法与应用
PCT/CN2020/111842 WO2021037166A1 (zh) 2019-08-28 2020-08-27 基于大肠杆菌的重组菌株及其构建方法与应用

Publications (1)

Publication Number Publication Date
BR112022002535A2 true BR112022002535A2 (pt) 2022-05-03

Family

ID=74683481

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022002535A BR112022002535A2 (pt) 2019-08-28 2020-08-27 Sequência nucleotídica, proteína recombinante, vetor recombinante, cepa recombinante, método de construção para a cepa recombinante, e, uso de uma sequência nucleotídica, de uma proteína recombinante, de um vetor recombinante ou de uma cepa recombinante

Country Status (6)

Country Link
US (1) US20220324919A1 (pt)
EP (2) EP4253569A3 (pt)
JP (1) JP7471395B2 (pt)
KR (1) KR20220034218A (pt)
BR (1) BR112022002535A2 (pt)
WO (1) WO2021037166A1 (pt)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102008063900A1 (de) * 2008-12-19 2010-06-24 Wacker Chemie Ag Verfahren zur fermentativen Herstellung von heterologen Proteinen mittels Escherichia coli
EP2628792A1 (de) * 2012-02-17 2013-08-21 Evonik Industries AG Zelle mit verringerter ppGppase-Aktivität
CN107267568A (zh) * 2017-07-21 2017-10-20 徐州工程学院 利用spoT基因缺失菌株通过发酵生产L‑氨基酸的方法
CN110804617B (zh) * 2019-09-27 2020-09-22 黑龙江伊品生物科技有限公司 一种kdtA基因改造的重组菌株及其构建方法与应用
CN110592109B (zh) * 2019-08-28 2020-10-09 黑龙江伊品生物科技有限公司 一种spoT基因改造的重组菌株及其构建方法与应用
CN110564742B (zh) * 2019-08-28 2020-08-21 黑龙江伊品生物科技有限公司 一种yebN基因改造的重组菌株及其构建方法与应用

Also Published As

Publication number Publication date
JP2022547010A (ja) 2022-11-10
KR20220034218A (ko) 2022-03-17
EP4253569A3 (en) 2024-04-24
EP3992293A4 (en) 2023-01-04
JP7471395B2 (ja) 2024-04-19
WO2021037166A1 (zh) 2021-03-04
EP3992293A1 (en) 2022-05-04
US20220324919A1 (en) 2022-10-13
EP4253569A2 (en) 2023-10-04

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