CN110559365B - Compound Chinese herbal medicine, feed and feed feeding method for promoting fat utilization in circulating water culture of tilapia flume - Google Patents
Compound Chinese herbal medicine, feed and feed feeding method for promoting fat utilization in circulating water culture of tilapia flume Download PDFInfo
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- 241000411851 herbal medicine Species 0.000 title claims abstract description 66
- 150000001875 compounds Chemical class 0.000 title claims abstract description 65
- 241000276707 Tilapia Species 0.000 title claims abstract description 29
- 230000001737 promoting effect Effects 0.000 title claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title abstract description 29
- 238000000034 method Methods 0.000 title abstract description 12
- 238000009360 aquaculture Methods 0.000 claims abstract description 26
- 244000144974 aquaculture Species 0.000 claims abstract description 26
- 230000003134 recirculating effect Effects 0.000 claims abstract description 14
- 235000009051 Ambrosia paniculata var. peruviana Nutrition 0.000 claims abstract description 10
- 235000003097 Artemisia absinthium Nutrition 0.000 claims abstract description 10
- 240000001851 Artemisia dracunculus Species 0.000 claims abstract description 10
- 235000017731 Artemisia dracunculus ssp. dracunculus Nutrition 0.000 claims abstract description 10
- 235000003261 Artemisia vulgaris Nutrition 0.000 claims abstract description 10
- 244000241838 Lycium barbarum Species 0.000 claims abstract description 10
- 235000015459 Lycium barbarum Nutrition 0.000 claims abstract description 10
- 235000015463 Lycium carolinianum Nutrition 0.000 claims abstract description 10
- 240000002853 Nelumbo nucifera Species 0.000 claims abstract description 10
- 235000006508 Nelumbo nucifera Nutrition 0.000 claims abstract description 10
- 235000006510 Nelumbo pentapetala Nutrition 0.000 claims abstract description 10
- 239000001138 artemisia absinthium Substances 0.000 claims abstract description 10
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 claims abstract description 10
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- 102000015303 Fatty Acid Synthases Human genes 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/254—Acanthopanax or Eleutherococcus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/282—Artemisia, e.g. wormwood or sagebrush
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/481—Astragalus (milkvetch)
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/62—Nymphaeaceae (Water-lily family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
- A61K36/815—Lycium (desert-thorn)
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract
The invention relates to a compound Chinese herbal medicine for promoting fat utilization in tilapia flume recirculating aquaculture, a feed and a feed feeding method, and belongs to the technical field of aquaculture management and feed feeding. The compound Chinese herbal medicine comprises the following components in percentage by mass: 15-25% of boxthorn leaves, 40-50% of acanthopanax stems, 15-25% of lotus leaves, 5-15% of oriental wormwood and 3-7% of astragalus stems and leaves. The feed prepared from the compound Chinese herbal medicines can enhance the absorption and utilization capacity of the tilapia mossambica in the water flowing tank recirculating aquaculture to the feed fat, reduce the deposition of the fat, effectively improve the absorption and utilization of the feed fat, reduce the deposition of the liver fat and reduce the occurrence probability of fatty liver and related diseases.
Description
Technical Field
The invention relates to aquaculture, in particular to the technical field of aquaculture management and feed feeding, and specifically relates to a compound Chinese herbal medicine for promoting fat utilization in circulating water culture of a tilapia flume, a feed and a feed feeding method.
Background
The gutter-water recirculating aquaculture integrates high-density aquaculture, online water quality monitoring and industrial management, can realize high-density intensive aquaculture of aquaculture objects, can perform biological purification treatment on residual aquaculture wastewater, and achieves self-purification and self-restoration of aquaculture water, thereby realizing effective and stable sustainable development of aquaculture industry. The method is widely popularized and applied in Jiangsu, Fujian, Zhejiang and other provinces, and the breeding objects mainly comprise pelteobagrus fulvidraco, crucian carp, bream, weever, grass carp and other economic varieties, so that better economic benefits are obtained.
While obtaining these favorable results, we should pay attention to the fact that the culture volume of the launder is limited, the culture area is 800-1000 square meters, and the culture density can reach 1-3 thousands of fishes. The fish body has a limited active area when fed by satiation. Meanwhile, the high-dissolved oxygen water body can increase the metabolic burden of the fish body for a long time, and excessive feed protein and fat can not be converted into energy required by growth in time, so that a large amount of fat is accumulated in the abdomen, liver and intestinal tract of the fish body. Thereby causing fat metabolism disorder, and the fish body is easy to form fatty liver and other fatty diseases. Therefore, how to promote the metabolism of the liver fat of the fish under the high dissolved oxygen and high-density culture becomes a key bottleneck which needs to be broken through urgently in the circulating water culture of the launder.
Disclosure of Invention
The invention aims to provide a compound Chinese herbal medicine, a feed and a feed feeding method for promoting fat utilization in circulating water culture of a tilapia flume. The feed provided by the invention can enhance the absorption and utilization capacity of the tilapia mossambica flume circulating water culture on feed fat, reduce the deposition of fat, effectively improve the absorption and utilization of the feed fat, reduce the deposition of liver fat, and reduce the occurrence probability of fatty liver and related diseases. Meanwhile, the Chinese herbal medicine composition added into the feed belongs to a green pollution-free environment-friendly feed additive, is easy to digest and absorb by the liver and intestinal tracts of fish bodies, has less residue in metabolites, and meets the requirement of adding green pollution-free compound Chinese herbal medicines.
The invention provides a compound Chinese herbal medicine for promoting fat utilization in circulating water culture of a tilapia flume, which comprises the following components in percentage by mass: 15-25% of boxthorn leaves, 40-50% of acanthopanax stems, 15-25% of lotus leaves, 5-15% of oriental wormwood and 3-7% of astragalus stems and leaves.
Preferably, the preparation method of the compound Chinese herbal medicine comprises the following steps: freeze drying, grinding and crushing the components, and screening the components with a 80-mesh screen.
The invention also provides a feed for promoting fat utilization in the circulating water culture of the tilapia water flowing tank based on the compound Chinese herbal medicines, which comprises basic ration and the compound Chinese herbal medicines.
Preferably, the basic ration comprises 26-30% of protein and 5-10% of fat by mass.
Preferably, the mass percentage of the compound Chinese herbal medicine in the feed is 0.05-0.2%.
Preferably, the mass percentage of the compound Chinese herbal medicine in the feed is 0.25-0.4%.
The invention also provides a feed feeding method for promoting fat utilization in tilapia flume recirculating aquaculture based on the compound Chinese herbal medicines or the feed in the technical scheme, which comprises the following steps:
the fish body specification is 10-150 g, and the fish body is fed with feed containing 0.05-0.2% of compound Chinese herbal medicines by mass percentage; the fish body specification is more than 150g, and the feed containing 0.25-0.4% of compound Chinese herbal medicine is fed; feeding three times a day, wherein the total feeding amount is 3-8% of the weight of the fish.
Preferably, the feeding is carried out three times a day for a period of 7:00, 11:30 and 16:00 respectively.
Preferably, the feeding amount corresponding to three times of feeding per day is respectively 30-40%, 40-50% and 20-30% of the total feeding amount.
Preferably, the number of times and amount of feeding is reduced or no feeding is performed on rainy days.
The invention provides a compound Chinese herbal medicine for promoting fat utilization in tilapia flume recirculating aquaculture. The compound Chinese herbal medicine provided by the invention can be added into feed to effectively improve the absorption and utilization of feed fat, reduce the fat deposition of blood and liver, reduce the occurrence probability of fatty liver and promote the healthy culture of tilapia; the compound Chinese herbal medicine formula belongs to a green environment-friendly preparation, the residue of the fish body and the negative effect on the environment are small, and the requirements of a green environment-friendly feed additive are met; the compound Chinese herbal medicine feed has the advantages of good fish body absorption efficiency, no muscle residue and no environmental pollution caused by fish body excrement.
Detailed Description
The invention provides a compound Chinese herbal medicine for promoting fat utilization in circulating water culture of a tilapia flume, which comprises the following components in percentage by mass: 15-25% of boxthorn leaves, 40-50% of acanthopanax stems, 15-25% of lotus leaves, 5-15% of oriental wormwood and 3-7% of astragalus stems and leaves. In the invention, the compound Chinese herbal medicine preferably comprises the following components in percentage by mass: 20% of boxthorn leaf, 45% of acanthopanax stem, 20% of lotus leaf, 10% of oriental wormwood and 5% of astragalus stem and leaf. The sources of the boxthorn leaf, the acanthopanax stem, the lotus leaf, the oriental wormwood and the astragalus root stem and leaf are not specially limited, and the conventional products sold in the market, such as the boxthorn leaf, the acanthopanax stem, the lotus leaf, the oriental wormwood and the astragalus root stem and leaf, which are well known by the technical personnel in the field can be adopted. According to the invention, the specific compatibility of the boxthorn leaves, the acanthopanax stems, the lotus leaves, the oriental wormwood and the astragalus stems and leaves can effectively promote the absorption of excessive fat in the feed, reduce the fat deposition in the liver and blood and keep the health state of the fish body.
In the present invention, the preparation method of the compound Chinese herbal medicine preferably comprises: freeze drying the components (Lycii folium, caulis Acanthopanacis Senticosi, folium Nelumbinis, herba Artemisiae Scopariae and radix astragali stem and leaf), grinding, and sieving with 80 mesh sieve. The freeze-drying and grinding operation method is not particularly limited in the present invention, and conventional operations well known to those skilled in the art may be adopted.
The invention also provides a feed for promoting fat utilization in the circulating water culture of the tilapia water flowing tank based on the compound Chinese herbal medicines, which comprises basic ration and the compound Chinese herbal medicines. In the invention, the basic ration preferably comprises 26-30% of protein and 5-10% of fat by mass.
The basic ration comprises 26-30% of protein by mass, and is more preferably 27%. The source of the protein is not particularly limited, and the protein can be obtained by adopting a conventional fish feed protein source. The feed protein level in the range can meet the nutritional requirements of tilapia in the circulating water culture of the launder.
The basic ration comprises 5-10% of fat by mass percentage, and more preferably 8%. The source of the fat is not particularly limited, and the fat source can be a conventional fish feed fat source. The feed fat level in the range of the invention can not only meet the nutritional requirements of tilapia in the circulating water aquaculture of the launder, but also save protein and promote the absorption and utilization of the protein.
In the invention, the mass percentage of the compound Chinese herbal medicine in the feed is preferably 0.05-0.2%. Can be adjusted according to different culture specifications, has less addition amount and does not influence the nutritional requirement of the fish body. In the invention, the mass percentage of the compound Chinese herbal medicine in the feed is more preferably 0.2%. When the fish body specification is 10-150 g, the feed containing 0.05-0.2% of compound Chinese herbal medicines is preferably fed, and the feed containing 0.2% of compound Chinese herbal medicines is more preferably fed.
In the invention, the mass percentage of the compound Chinese herbal medicine in the feed is preferably 0.25-0.4%. In the adding range of the compound Chinese herbal medicine, the compound Chinese herbal medicine is less added in the feed composition in the adult tilapia culture stage, so that the nutritional requirement of a fish body is not influenced, and the problem of fish body residue of the additive is not caused. In the invention, the mass percentage of the compound Chinese herbal medicine in the feed is more preferably 0.3%. When the fish body specification is more than 150g, the feed containing 0.25-0.4% of compound Chinese herbal medicines is preferably fed in the invention, and more preferably 0.3%.
The invention also provides a feed feeding method for promoting fat utilization in tilapia flume recirculating aquaculture based on the feed, which comprises the following steps:
the fish body specification is 10-150 g, and the fish body is fed with feed containing 0.05-0.2% of compound Chinese herbal medicines by mass percentage; the fish body specification is more than 150g, and the feed containing 0.25-0.4% of compound Chinese herbal medicine is fed; feeding three times a day, wherein the total feeding amount is 3-8% of the weight of the fish. In the present invention, the feeding is preferably carried out three times a day at 7:00, 11:30 and 16:00 times, respectively. In the invention, the feeding amount corresponding to three times of feeding per day is respectively 30-40%, 40-50% and 20-30% of the total feeding amount. In the invention, a small amount of feed is fed in the morning or afternoon preferably in rainy days in cloudy days, the feeding amount is 20-40% of the total feeding amount, and an aerator is continuously started to keep sufficient dissolved oxygen in water. Heavy rain or persistent overcast rain, preferably without feeding. Proper feeding is carried out at different feeding time, so that the feed can be optimally utilized, and the feed waste and fat deposition are reduced. Meanwhile, the method is helpful for reducing the metabolic burden and survival pressure of the fish body caused by overeating.
The following describes the compound Chinese herbal medicine, feed and feed feeding method for promoting fat utilization in tilapia flume recirculating aquaculture in detail with reference to specific embodiments, and the technical scheme of the invention includes but is not limited to the following embodiments.
Example 1
A set of feed compatibility and feeding mode for promoting fat utilization in tilapia flume recirculating aquaculture comprises the following steps:
step 1, selecting 6 water flowing grooves with the square meters of 800 in Jiangsu Yangyang, and stocking 20000 tail tilapia in each water flowing groove, wherein the fish body specification is about 250 g. 3 flumes were used as control groups, and a conventional commercial fish feed was prepared before the experiment, wherein the protein level was 29% and the fat level was 4%. 3 flumes are used as experimental groups, basic daily ration feed with the protein level of 27% and the fat level of 7% is prepared before experiments, and compound Chinese herbal medicines are added. In the two groups of feeds, the feed composition is the same, the levels of protein and fat in the feeds are adjusted by fish meal and soybean oil, and the specific feed formula table is shown in table 1.
TABLE 1 feed Components and Nutrition levels
Step 2, preparing compound Chinese herbal medicines: every 100g of the compound Chinese herbal medicines comprise 20g of boxthorn leaves, 45g of acanthopanax stems, 20g of lotus leaves, 10g of oriental wormwood and 5g of astragalus stem leaves in parts by weight; in the step 2, the compound Chinese herbal medicine is prepared by the following steps: freeze drying, grinding, sieving with 80 mesh sieve, and storing at 4 deg.C.
Step 3, adding compound Chinese herbal medicines: according to the weight portion, each 100g of feed contains 0.25g of compound Chinese herbal medicine respectively, and 0.25% of compound Chinese herbal medicine addition group is prepared;
step 4, feeding time is as follows: feeding three times a day, wherein the feeding amount is 7:00, 11:30 and 16:00 respectively, the feeding amount is 5 percent of the weight of the fish, and the feeding amount of the three times is 30 percent, 40 percent and 30 percent of the total feeding amount per day respectively. In the rainy days in cloudy days, a small amount of feed is fed in the morning or afternoon, the feeding amount is 20 percent of the total feeding amount, and the aerator is continuously started to keep the dissolved oxygen in the water body sufficient. And in heavy rain or persistent overcast and rainy days, the feeding is not required. Specific results are shown in tables 2 to 4.
TABLE 2 influence of the addition of compound Chinese herbal medicine in the feed on the growth and fat deposition of adult tilapia
| Control group | Experimental group | |
| Initial body weight (g) | 254.33 | 255.91 |
| Terminal body weight (g) | 485.17 | 488.12 |
| Liver weight (g) | 16.33 | 12.11 |
| Liver triglyceride (mmol/L) | 4.75 | 3.31 |
| Liver Cholesterol (mmol/L) | 3.88 | 2.70 |
| Serum triglyceride (mmol/L) | 3.35 | 2.16 |
| Serum cholesterol (mmol/L) | 6.58 | 4.37 |
TABLE 3 primer sequences
Fatty acid synthase and lipoprotein lipase are key enzymes of the fat synthesis and metabolism pathways. Fatty acid synthetase plays an important role in the production and deposition of animal body fat. Lipoprotein lipase is a key enzyme in the regulation of fat metabolism. In the present study, the above primer sequences were used to perform PCR amplification, and the expression characteristics of fatty acid synthetase and lipoprotein lipase in the experimental group and the control group were analyzed, and the related experimental results are shown in Table 4.
TABLE 4 influence of the addition of compound Chinese herbal medicines to the feed on the expression of liver lipid metabolism enzyme gene of tilapia mossambica
As can be seen from tables 2 and 4, the contents of triglyceride and cholesterol in liver and serum of the tilapia in the experimental group are obviously lower than those in the control group, and the gene expression activities of lipoprotein lipase and fatty acid synthetase in liver of the experimental group are obviously higher than those in the control group. The compound Chinese herbal medicine provided by the invention is added into the feed, so that the liver fat metabolism can be obviously regulated and controlled, and the serum and liver fat deposition can be reduced.
Example 2
A set of feed compatibility and feeding mode for promoting fat utilization in tilapia flume recirculating aquaculture comprises the following steps:
step 1, selecting 6 water flowing grooves with the square meters of 800 in Changzhou of Jiangsu, and breeding 15000 tilapia mossambica in each water flowing groove, wherein the fish body specification is about 50 g. Preparing a basic daily ration feed with the protein level of 30% and the fat level of 7%; 3 launders are used as a control group, and only basic daily ration feed is fed; 3 launders were used as experimental groups to feed basic ration feed with compound Chinese herbal medicine.
Step 2, preparing compound Chinese herbal medicines: every 100g of the compound Chinese herbal medicines comprise 20g of boxthorn leaves, 45g of acanthopanax stems, 20g of lotus leaves, 10g of oriental wormwood and 5g of astragalus stem leaves in parts by weight; in the step 2, the compound Chinese herbal medicine is prepared by the following steps: freeze drying, grinding, sieving with 80 mesh sieve, and storing at 4 deg.C.
Step 3, adding compound Chinese herbal medicines: according to the weight portion, each 100g of feed contains 0.2g of compound Chinese herbal medicine respectively, and 0.2% of compound Chinese herbal medicine addition group is prepared;
and 4, feeding time is as follows: feeding three times a day, wherein the feeding amount is 7:00, 11:30 and 16:00 respectively, the feeding amount is 7% of the weight of the fish, and the feeding amount of the three times is 40%, 40% and 20% of the total feeding amount per day respectively. And in rainy days, the feeding frequency and the feeding amount are reduced, or feeding is not performed. The culture period is 60 days. Specific results are shown in table 5:
TABLE 5 influence of the addition of compound Chinese herbal medicine in the feed on the growth and fat deposition of young tilapia
| Control group | Experimental group | |
| Initial body weight (g) | 50.34 | 48.72 |
| Terminal body weight (g) | 312.88 | 315.91 |
| Liver weight (g) | 13.67 | 9.45 |
| Liver triglyceride (mmol/L) | 4.82 | 3.73 |
| Liver Cholesterol (mmol/L) | 3.69 | 2.84 |
| Serum triglyceride (mmol/L) | 3.61 | 2.53 |
| Serum cholesterol (mmol/L) | 5.19 | 3.62 |
Compared with a control group, the growth of the experimental group has no obvious difference, then the liver weight of the experimental group is obviously lower than that of the control group, and the contents of triglyceride and cholesterol in serum and liver are also obviously lower than that of the control group.
In conclusion, after the treatment of the invention, in the circulating water culture of the flowing water tank, tilapia ingests the compound Chinese herbal medicine of the invention, so that the contents of liver, serum cholesterol and triglyceride can be obviously reduced, and the weight of the liver is reduced. Effectively relieves liver metabolic stress caused by high density and high dissolved oxygen in the water flowing tank recirculating aquaculture, ensures the aquaculture efficiency and benefit of the water flowing tank recirculating aquaculture tilapia, and improves the healthy aquaculture of the tilapia.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Sequence listing
<110> research center of freshwater fishery of Chinese aquatic science research institute
<120> compound Chinese herbal medicine, feed and feed feeding method for promoting fat utilization in tilapia flume recirculating aquaculture
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Claims (6)
1. The compound Chinese herbal medicine for promoting fat utilization in tilapia flume recirculating aquaculture is characterized by comprising the following components in percentage by mass: 15-25% of boxthorn leaves, 40-50% of acanthopanax stems, 15-25% of lotus leaves, 5-15% of oriental wormwood and 3-7% of astragalus stems and leaves.
2. The compound Chinese herbal medicine according to claim 1, wherein the preparation method of the compound Chinese herbal medicine comprises: freeze drying, grinding and crushing the components, and screening the components with a 80-mesh screen.
3. The feed for promoting the utilization of fat in the recirculating aquaculture of the tilapia aquariums based on the compound Chinese herbal medicines of claim 1 is characterized by comprising basic ration and the compound Chinese herbal medicines.
4. The feed according to claim 3, wherein the basal diet comprises 26-30% by mass of protein and 5-10% by mass of fat.
5. The feed as claimed in claim 3, wherein the compound Chinese herbal medicine is contained in the feed in an amount of 0.05-0.2% by mass.
6. The feed as claimed in claim 3, wherein the compound Chinese herbal medicine is contained in the feed in an amount of 0.25-0.4% by mass.
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