KR102276217B1 - Composition of feed stuff for sea cucumber containing asthma improving substance and manufacturing method thereof - Google Patents

Abstract

The present invention relates to a composition for aquaculture feed for sea cucumber, and more particularly, to a composition for aquaculture feed of sea cucumber ( Apostichopus japonicus ) comprising a marine microalgae powder with enhanced asthma improvement effect as an active ingredient, and a method for preparing the same.

Description

Sea cucumber feed composition containing asthma improving substance and manufacturing method thereof {Composition of feed stuff for sea cucumber containing asthma improving substance and manufacturing method thereof}

The present invention relates to a sea cucumber feed composition comprising an asthma improving substance, and more particularly, to a sea cucumber feed composition comprising an asthma improving substance, and a method for preparing the same.

Sea cucumber ( Apostichopus japonicus ) is an expensive food in China, but due to recent marine pollution and habitat destruction caused by coastal reclamation, production in China is insufficient. Therefore, in Korea, it has been designated as one of the top 10 strategic varieties for export of aquatic products, and is spurring sea cucumber farming such as the development of sea cucumber seed production technology and processing technology development, as well as the sea cucumber cluster formation project. In particular, sea cucumber has been used for arthritis, high blood pressure, wound healing and recovery after surgery in China since ancient times, and is known to be effective in improving asthma. Therefore, in order to increase the added value of sea cucumbers, the production of high value-added sea cucumbers with functionality is required, away from the simple processing and exporting of farmed sea cucumbers.

On the other hand, asthma (asthma) is a type of allergic respiratory disease that suffers from about 300 million people worldwide, and the number of deaths due to it is about 250,000 a year, and the number continues to increase. In addition, asthma is one of the diseases of increasing interest by the World Health Organization (WHO) as the age of onset gradually decreases due to factors such as secondhand smoke, fine dust, yellow dust, eating habits, and weakening of children's immune system. In this regard, Korean Patent Application Laid-Open No. 2013-0009425 discloses a composition for a sea cucumber feed using an eco-friendly material and a method for manufacturing the same.

However, the prior art is not a feed composition for the production of aquaculture sea cucumbers that exhibits functionality as a health food, only considering the quantitative increase by feeding, as manufacturing a sea cucumber feed in a solidified form using seaweed.

The present invention is to solve the above problems, and an object of the present invention is to provide a feed composition and a method for producing the same for producing a sea cucumber which has excellent growth compared to the conventional sea cucumber culture method and contains a large amount of an asthma improving substance. However, these problems are exemplary, and the scope of the present invention is not limited thereto.

According to one aspect of the present invention, 10 to 20 parts by weight of powder of one or two or more algae selected from the group consisting of brown seaweed, kelp and nanoclops (Nannochloropsi s sp.), Sargassum thunbergii 10 to 20 parts by weight, 5 to 10 parts by weight of soybean meal, 5 to 10 parts by weight of shell fish powder, 1 to 3 parts by weight of shell powder, 1-3 parts by weight of calcium phosphate parts, 3 to 8 parts by weight of yeast extract, 2 to 6 parts by weight of soyabean lecithin, 0.2 to 0.7 parts by weight of minerals, 0.2 to 0.7 parts by weight of vitamins, and sea mud ( sea mud), comprising 20 to 60 parts by weight, is provided a feed composition for aquaculture of sea cucumbers for the production of sea cucumbers with enhanced asthma improvement effect.

According to another aspect of the present invention, 10 to 20 parts by weight of powder of one or two or more algae selected from the group consisting of brown seaweed, kelp and nanoclops (Nannochloropsi s sp.), Sargassum thunbergii 10 to 20 parts by weight, soybean meal 5 to 10 parts by weight, shell fish powder 5 to 10 parts by weight, shell powder 1 to 3 parts by weight, calcium phosphate 1-3 parts by weight parts by weight, 3 to 8 parts by weight of yeast extract, 2 to 6 parts by weight of soyabean lecithin, 0.2 to 0.7 parts by weight of minerals, 0.2 to 0.7 parts by weight of vitamins, and sea mud (sea mud) mixing 20 to 60 parts by weight to prepare a mixture; And there is provided a method for producing a feed composition for aquaculture of sea cucumbers with enhanced asthma improvement effect comprising the step of drying and solidifying the mixture.

As described above, the sea cucumber feed composition containing the asthma improving substance of the present invention and its manufacturing method use brown algae powder with a high glycolipid content as the main raw material, and feed to farmed sea cucumber, which is known to have low or almost no asthma improvement efficacy compared to wild sea cucumber. Thus, it is possible to realize the effect of producing sea cucumbers containing a large amount of asthma improving substances. Of course, the scope of the present invention is not limited by these effects.

1 is a schematic diagram of a circulation filtration system (RAS) designed for a sea cucumber breeding experiment using the experimental feed of the present invention.
2 is a photograph of a circulation filtration system (RAS) designed for a sea cucumber breeding experiment using the experimental feed of the present invention.
3 is a graph analyzing the specific growth rate (SGR) of sea cucumbers fed the experimental feed of the present invention. Lowercase letters indicate significant differences (P <0.05) between the same treatment groups, and bars indicate standard errors.
4 is a graph analyzing the intake rate (IR) of sea cucumbers fed the experimental feed of the present invention. Lowercase letters indicate significant differences (P <0.05) between the same treatment groups, and bars indicate standard errors.
5 is a graph analyzing the fecal production rate (FPR) of sea cucumbers fed the experimental feed of the present invention. Lowercase letters indicate significant differences (P <0.05) between the same treatment groups, and bars indicate standard errors.
6 is a graph analyzing the dietary conversion efficiency (FCE) of sea cucumbers fed the experimental feed of the present invention. Lowercase letters indicate significant differences (P <0.05) between the same treatment groups, and bars indicate standard errors.
7 is a photograph showing the manufacturing process of an allergic respiratory disease-induced mouse model after administration of an extract of sea cucumber fed with the experimental feed of the present invention.
8 is a graph analyzing the expression level of the anti-inflammatory cytokine, IL-10, according to the administration of the extract of sea cucumber fed the experimental feed of the present invention to a mouse model. Lowercase letters indicate significant differences (P <0.05) between the same treatment groups, and bars indicate standard errors.

Definition of Terms:

As used herein, the term "sea cucumber ( Apostichopus japonicus )" is called a stony sea cucumber, and according to the biological classification system, it belongs to Echinodermata, Holothuroidea, Stichopodiae, and Apostichopus. Since it contains a large amount of saponin and chondroitin, which are health functional substances, it has been called ginseng of the sea since ancient times.

As used herein, the term "Interleukin 10 (IL-10)" is also known as human cytokine synthesis inhibitory factor (CSIF) and is an anti-inflammatory cytokine encoded by the IL-10 gene. IL-10 signals through a receptor complex consisting of two IL-10 receptor-1 and two IL-10 receptor-2 proteins.

DETAILED DESCRIPTION OF THE INVENTION:

According to one aspect of the present invention, 10 to 20 parts by weight of powder of one or two or more algae selected from the group consisting of brown seaweed, kelp and nanoclops (Nannochloropsi s sp.), Sargassum thunbergii 10 to 20 parts by weight, 5 to 10 parts by weight of soybean meal, 5 to 10 parts by weight of shell fish powder, 1 to 3 parts by weight of shell powder, 1-3 parts by weight of calcium phosphate parts, 3 to 8 parts by weight of yeast extract, 2 to 6 parts by weight of soyabean lecithin, 0.2 to 0.7 parts by weight of minerals, 0.2 to 0.7 parts by weight of vitamins, and sea mud ( sea mud), comprising 20 to 60 parts by weight, is provided a feed composition for aquaculture of sea cucumbers for the production of sea cucumbers with enhanced asthma improvement effect.

The feed composition for aquaculture of sea cucumbers is more preferably a powder of one or two or more algae selected from the group consisting of brown seaweed, kelp, and nanoclop s sp. 13 to 17 parts by weight, worms 13 to 17 parts by weight, 6 to 9 parts by weight of soybean meal, 6 to 9 parts by weight of shellfish powder, 1.5 to 2.5 parts by weight of shell powder, 1.5 to 2.5 parts by weight of calcium phosphate, 4 to 7 parts by weight of yeast extract, 3 to 5 parts by weight of soybean lecithin, It may contain 0.3 to 0.6 parts by weight of minerals, 0.3 to 0.6 parts by weight of vitamins, and 30 to 50 parts by weight of sea mud.

The feed composition for sea cucumber aquaculture is most preferably 14 to 16% by weight of powder of one or more algae selected from the group consisting of kelp and Nanochloropsi s sp., and 14 to 16% by weight of worms , soybean meal 7-9 wt%, shellfish powder 7-9 wt%, shell powder 1.5-2.5 wt%, calcium phosphate 1.5-2.5 wt%, yeast extract 4-6 wt%, soybean lecithin 3-5 wt%, mineral 0.4 to 0.6% by weight, vitamins 0.4 to 0.6% by weight, and the balance sea mud.

According to another aspect of the present invention, 10 to 20 parts by weight of powder of one or two or more algae selected from the group consisting of brown seaweed, kelp and nanoclops (Nannochloropsi s sp.), Sargassum thunbergii 10 to 20 parts by weight, soybean meal 5 to 10 parts by weight, shell fish powder 5 to 10 parts by weight, shell powder 1 to 3 parts by weight, calcium phosphate 1-3 parts by weight parts by weight, 3 to 8 parts by weight of yeast extract, 2 to 6 parts by weight of soyabean lecithin, 0.2 to 0.7 parts by weight of minerals, 0.2 to 0.7 parts by weight of vitamins, and sea mud (sea mud) mixing 20 to 60 parts by weight to prepare a mixture; And there is provided a method for producing a feed composition for aquaculture of sea cucumbers with enhanced asthma improvement effect comprising the step of drying and solidifying the mixture.

Most sea cucumbers are deposit feeders that consume sediment as organic matter. As the production of sea cucumber culture increases worldwide, sea cucumber culture is focused only on the quantitative increase by feeding the compound feed, and the quality of production as a health food is neglected. In addition, it has been shown that farmed sea cucumbers have lower or no asthma improvement effects than wild-caught sea cucumbers. Accordingly, the present inventors have made a conscious effort to produce sea cucumbers containing substances that can improve asthma in cultured sea cucumbers. As a result, brown algae flour (seaweed, kelp) or omega-3 fatty acids (seaweed, kelp) or omega-3 fatty acids ( By providing seawater chlorella (Nannochloropsis ) containing EPA) as a main raw material, the present invention was developed by developing a feed composition and a method for producing sea cucumber that has excellent growth compared to the conventional sea cucumber culture method and contains a large amount of asthma improving substances. completed.

Hereinafter, the present invention will be described in more detail through examples. However, the present invention is not limited to the embodiments disclosed below, but can be implemented in various different forms, and the following examples allow the disclosure of the present invention to be complete, and the scope of the invention to those of ordinary skill in the art It is provided to fully inform

Example 1: Experimental feed stuff

The present inventors prepared a total of 6 types of experimental feeds including basic feed or asthma improvement candidates to prepare a sea cucumber feed composition containing an asthma improving substance. Specifically, a feed prepared with 100% of the basic feed was used as a control group (control), the experimental group in which 15% of Ulva lactuca powder was added to 85% of the basic feed (Diet 1), and 85% of the basic feed with seaweed flour ( Undaria) pinnatifida powder) 15% (Diet 2), 85% of basic feed with 15% of Laminaria japonica powder (Diet 3), and 85% of basic feed with microalgae ( Schizochytrium powder) 15 % was added (Diet 4), and an experimental group (Diet 5) in which 15% of seawater chlorella powder (Nannochloropsis powder) was added to 85% of the basic feed (Diet 5) was prepared. Among the seaweeds of the experimental group, brown seaweed, seaweed, and kelp were purchased from commercially available dried raw materials, ground into 200-μm mesh fine powder, and added to the feed. Shijo chitrium and seawater chlorella were purchased from aquaculture feed vendors and frozen. After drying and powdering, it was stored frozen at -20°C and used. Experimental animals and feed performance tests were performed using the experimental feed prepared above for 9 weeks in the Marine Biology and Aquaculture Laboratory of Gyeongsang National University.

Composition of a total of 6 experimental feeds including basic feed or asthma improvement candidates ingredient control Diet 1 Diet 2 Diet 3 Diet 4 Diet 5 Ulva lactuca powder 0 15 0 0 0 0 Undaria pinnatifida powder 0 0 15 0 0 0 Laminaria japonica powder 0 0 0 15 0 0 Schizochytrium powder 0 0 0 0 15 0 Nanochloropsis powder 0 0 0 0 0 15 Wheat flour 15 0 0 0 0 0 basic ingredients Sargassum thunbergii powder 15 15 15 15 15 15 Soybean meal 8 8 8 8 8 8 shellfish powder 8 8 8 8 8 8 shell powder 2 2 2 2 2 2 Calcium phosphate 2 2 2 2 2 2 yeast extract 5 5 5 5 5 5 Soyabean lecithin 4 4 4 4 4 4 Mineral 0.5 0.5 0.5 0.5 0.5 0.5 Vitamin 0.5 0.5 0.5 0.5 0.5 0.5 sea mud 40 40 40 40 40 40

Example 2: General component analysis

The present inventors performed general component analysis on the sea cucumber feeds of Comparative Examples and Examples 1 to 5 prepared above. Specifically, the general component analysis was analyzed by the AOAC (Association of Official Analytical Chemists. Washington DC., AOAC. Official method of analysis. 16th ed. USA ., 1-43, 1995) method, crude protein was analyzed by Kjeldahl method, and crude fat was analyzed by the Kjeldahl method. The Bligh and Dyer extraction method (1959) was followed by using a solution obtained by mixing chloroform and methanol in a ratio of 2:1 as a solvent. Moisture was measured after drying in a dry oven at 105° C. for 6 hours by atmospheric heating and drying method, and the crude powder was quantified after burning for 4 hours in a furnace at 600° C. by dry ashing.

As a result, in the case of the control group, crude protein excluding water was 17.7%, lipid 3.4%, and raw meal 41.2%, and in Diet 1 sea cucumber feed, crude protein was 17%, crude lipid 3.7%, and raw material 44.8%, Sea cucumber feed of Diet 2 was found to contain 17.5% crude protein, 3.1% crude lipid, and 45.5% raw meal. In addition, the sea cucumber feed of Diet 3 showed the highest crude protein content among the experimental groups with 17.1% crude protein, 3.1% crude lipid, and 45.8% crude lipid, and the sea cucumber feed of Diet 4 had 17.6% crude protein, 7.4% crude lipid, and 41.5 crude meal. % showed the highest crude lipid content among the experimental groups, and Diet 5 sea cucumber feed showed the highest crude protein content among the experimental groups with 19.6% crude protein, 5.6% crude lipid, and 42.1% crude meal. The general component analysis results are summarized in Table 2 below.

Analysis of general components of a total of 6 experimental feeds General composition (%) control Diet 1 Diet 2 Diet 3 Diet 4 Diet 5 crude protein 17.74 17.03 17.48 16.87 17.59 19.64 geologic 3.39 3.72 3.11 3.32 7.44 5.64 views 41.20 44.80 45.55 45.85 41.5 42.10

Example 3: Sea cucumber breeding experiment

The present inventors performed a sea cucumber breeding experiment using a total of 6 types of experimental feed prepared in Example 1. Specifically, Apostichopus japonicus used in the present invention has an average weight of 3.0 g, and 1,000 of them are sold from a seedling producer in Sanyang-myeon, Tongyeong-si, Gyeongnam, transported to the laboratory, stocked in a 1,000 L tank, and then adapted to the experimental environment. Preliminary breeding was carried out for 5 weeks while supplying basal feed in seawater at 17°C and 32 psu. After the dead individuals were disposed of, 480 healthy sea cucumbers with an average weight of 3.0±0.1 g were selected and placed in each 96 L square tank. The breeding experiment was repeated 4 times for each experimental group in the circulating filtration system (RAS) designed for accommodating 20 specimens and rearing sea cucumbers (20 animals per tank x 4 repetitions x 6 feed groups = 480 animals). The breeding water circulation rate in each experimental tank was adjusted to 20 cycles/day, and the breeding environment during the experiment period was water temperature of 18°C, salt 32 psu, pH 7.7-8.3, dissolved oxygen 5.0-7.0 mg/L, and total ammonia of 0.25 ppm or less. It was maintained within the range, and the daily feed amount was 5% of the body weight of sea cucumbers once a day (17:00) while sufficiently supplying for 9 weeks (Figs. 1 and 2).

Example 4: Sample Collection

The present inventors collected 24 sea cucumbers as an initial sample before starting the experiment, and fed the sea cucumbers once a day (about 1700 hours) for the duration of the experiment. Uneaten feed was collected with a siphon after 24 hours and dried to a constant weight at 65°C. Sea cucumber excrement was also collected with a siphon once a day (1600 hours), and the excrement was dried at a constant weight at 65° C., and then the excrement from each tank was collected for further analysis. At the end of the 9-week experiment, all experimental sea cucumbers were stopped from feeding for 2 days to remove the intestines, weighed, and dried at 65°C until they reached a constant weight.

Example 5: Data Calculation

Survival rate (SR), specific growth rate (SGR), ingestion rate (IR), feces production rate (feces) of the experimental group according to the feeding of the sea cucumber feed prepared according to an embodiment of the present invention The production rate (FPR), and food conversion efficiency (FCE) were calculated by the following formula.

Survival Rate (SR):

In the above formula, N2 is the final number of surviving individuals, and N1 is the initial number of individuals.

Specific Growth Rate (SGR):

In the above formula, W ₁ is the initial weight, W ₂ is the final weight, I is the amount of feed fed , and T is the breeding period.

Intake Rate (IR):

In the above formula, I represents the dry weight of the total feed consumed.

Fecal Production Rate (FPR):

In the above formula, F represents the dry weight of excrement.

Dietary Conversion Efficiency (FCE):

In the above formula, W ₁ is the initial weight, and W ₂ is the final weight.

For all statistical analysis, one-way ANOVA test was performed using the SPSS 16.0 program, and then the significance (P<0.05) between the means was tested with the Duncan smultiple rang test (Duncan, 1995).

Example 6: Preparation of sea cucumber extract

To prepare a sea cucumber extract, the experimental sea cucumber was washed, internal organs were removed, and cut into small pieces and homogenized. Then, 150 g of the sample was boiled in 300 ml of distilled water for 20 minutes. After removing the solid material from the water, the boiled water was evaporated using a microwave until the mixture was reduced by 50%, the extract was centrifuged at 500 x g for 10 minutes, and 5 times the volume of 100% ethyl alcohol was added to the supernatant. The supernatant was removed after incubation at 20 °C for 24 hours. The extraction pellet was washed with 70% ethyl alcohol, centrifuged under the same conditions as above, the supernatant was removed, the pellet was evaporated in vacuo, and the final extract was prepared by resuspending the pellet in 20 ml of distilled water (Lee et al. , Am J Chin Med. 44:1663-74. 2016).

Example 7: IL-10 gene expression analysis

In order to analyze IL-10 gene expression, the present inventors stimulated mouse splenocytes for 2 hours using 10 μg/ml of an experimental feed-fed sea cucumber extract. Thereafter, total RNA was isolated with Qiazol reagent (Qiagen Science, USA) according to the manufacturer's instructions. According to the manufacturer's instructions, 2 μg of total RNA was transcribed using M-MLV reverse transcriptase (Promega, USA) and IL- 10 mRNA expression levels were synthesized by real-time PCR using iCycler™ (Bio-Rad Laboratories, Hercules, CA, USA). In addition, GAPDH was used as a reference gene. The primer information used in the real-time PCR furnace is summarized in Table 3 below.

IL-10 and GAPDH primer sequences primer base sequence (5'-->3') SEQ ID NO: GAPDH F TAC CCC CAA TGT GTC CGT C One GAPDH R AAG AGT GGG AGT TGC TGT TGA AG 2 IL-10 F GCT ATG CTG CCT GGT CTT ACT G 3 IL-10R TCC AGC TGG TCC TTT GTT TG 4

Experimental Example 1: Analysis of survival and growth

After feeding a total of 6 types of feed prepared according to an embodiment of the present invention, the performance of the feed was analyzed. All sea cucumbers of the present invention were alive until the end of the 9-week feeding period, and the growth ability of sea cucumbers was significantly different depending on the type of feed. The final dry and wet weight of sea cucumber was the highest in the Diet 1 experimental group and the lowest in the control group (see Table 4) (P <0.05).

In addition, the highest specific growth rate (SGR, 1.58 % d ^-1 ) was observed in sea cucumbers fed the Diet 1 feed, but the control group was significantly lower than Diet 1 and Diet 3 (P <0.05) and Diet 5 was There was no significant difference compared with Diet 4 (P>0.05) ( FIG. 3 ). Wet and dry weights according to the feed fed to the sea cucumber are summarized in Table 4 below.

Performance analysis results according to the feed of sea cucumber feed Item Early
Wet Weight(g) Early
Dry Weight(g) final
Wet Weight(g) final
Dry Weight(g) control 3.04±0.09 0.28 5.07±0.92 0.47 Diet 1 2.93±0.12 0.27 7.65±0.85 0.71 Diet 2 2.94±0.11 0.27 5.94±0.62 0.55 Diet 3 3.03±0.07 0.28 7.27±0.45 0.67 Diet 4 2.92±0.10 0.27 6.16±0.15 0.57 Diet 5 3.02±0.06 0.28 7.05±0.18 0.65

Experimental Example 2: Intake rate (IR), fecal production rate (FPR) and diet conversion efficiency (FCE)

The present inventors analyzed the intake rate, feces production rate and diet conversion efficiency after feeding the experimental feed to sea cucumbers. Among the experimental groups, Diet 3 and Diet 1 showed significantly higher intake rates (0.62 and 0.59 gg ^-1 d ^-1 , respectively) and fecal production rates (0.53 and 0.52 gg ^-1 d ^-1 , respectively) than sea cucumber fed other feeds ( P<0.05). On the other hand, the sea cucumber of the control group showed the lowest intake rate (0.32 gg ^-1 d ^-1 ) (P <0.05) and fecal production rate (0.26 gg ^-1 d ^-1 ) (P <0.05) among all experimental groups ( FIGS. 4 and 5 ) ( FIGS. 4 and 5 ) ). In addition, sea cucumber fed with Diet 2 showed the lowest dietary conversion efficiency (2.21%), but sea cucumber fed with Diet 5 showed significantly higher dietary conversion efficiency (3.74%) compared to sea cucumber fed with other experimental feeds ( 6) (P <0.05).

Experimental Example 3: IL-10 gene expression level

The present inventors analyzed the expression level of the IL-10 gene, an anti-inflammatory cytokine, in order to select seaweed suitable for sea cucumber feed containing an asthma improving substance.

Specifically, before inducing asthma using an allergic respiratory disease induction mouse model, 200 μl of sea cucumber extract (100 mg/ml) fed with 6 experimental feeds of the present invention was orally administered 7 times at 2-day intervals per mouse. After 1 day, 75 μg of ovalbumin (OVA) and 2 μg of alum were intraperitoneally injected once a day for 2 days, and after 6 days, once a day for 2 days, the same amount of eggs as above Albumin and alum were injected intraperitoneally. After 6 days, 50 μg of egg albumin was administered intranasally once a day for 2 days, and 6 days later, the same amount of egg albumin was administered intranasally once a day for 2 days to induce asthma. Thereafter, to determine whether asthma was induced, airway resistance was measured using methacholine, and after sacrificing mice, splenocytes were extracted to measure the expression of IL-10 gene, an asthma improving substance. (Fig. 7).

As a result, the expression level of IL-10 gene, which is an asthma improving substance, was significantly increased in the Diet 1, Diet 3 and Diet 5 experimental groups, and was the highest when Diet 1, that is, brown seaweed ( Ulva lactuca ) was fed. However, the control group, Diet 2 and Diet 4 experimental groups did not increase and did not show a significant difference (FIG. 8). The above results suggest that the seaweed contained in Diet 1 (sea cucumber), Diet 3 (kelp), and Diet 5 (chlorella), which are sea cucumber feeds of the present invention, can up-regulate IL-10 gene expression.

In conclusion, the sea cucumber feed composition containing the asthma improving substance (IL-10) of the present invention and its manufacturing method have improved growth of sea cucumber and contain a large amount of the asthma improving substance compared to the conventional sea cucumber culture method that contains almost no asthma improving substance. Because it contains sea cucumber, it can be used as feed for aquaculture of various aquatic organisms.

Although the present invention has been described with reference to the above-described embodiment, it will be understood that this is merely exemplary, and that those skilled in the art may make various modifications and equivalent other embodiments therefrom. Therefore, the true technical protection scope of the present invention should be determined by the technical spirit of the appended claims.

<110> INDUSTRY-ACADEMIC COOPERATION FOUNDATION GYEONGSANG NATIONAL UNIVERSITY <120> Composition of feed stuff for sea cucumber containing asthma improving substance and manufacturing method thereof <130> PD18-5660 <160> 4 <170> KoPatentIn 3.0 <210> 1 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> GAPDH F <400> 1 tacccccaat gtgtccgtc 19 <210> 2 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> GAPDH R <400> 2 aagagtggga gttgctgttg aag 23 <210> 3 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> IL-10 F <400> 3 gctatgctgc ctggtcttac tg 22 <210> 4 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> IL-10 R <400> 4 tccagctggt cctttgtttg 20

Claims (2)

10 to 20 parts by weight of powder of one or more algae selected from the group consisting of brown seaweed and Nanochloropsi s sp., Sargassum thunbergii 10 to 20 parts by weight, soybean meal 5 to 10 parts by weight, 5 to 10 parts by weight of shell fish powder, 1 to 3 parts by weight of shell powder, 1 to 3 parts by weight of calcium phosphate, 3 to 3 parts by weight of yeast extract 8 parts by weight, 2 to 6 parts by weight of soyabean lecithin, 0.2 to 0.7 parts by weight of minerals, 0.2 to 0.7 parts by weight of vitamins, and 20 to 60 parts by weight of sea mud , Sea cucumber aquaculture feed composition for the production of sea cucumber with enhanced asthma improvement effect. 10 to 20 parts by weight of powder of one or more algae selected from the group consisting of brown seaweed and Nanochloropsi s sp., Sargassum thunbergii 10 to 20 parts by weight, soybean meal 5 to 10 parts by weight, 5 to 10 parts by weight of shell fish powder, 1 to 3 parts by weight of shell powder, 1 to 3 parts by weight of calcium phosphate, 3 to 3 parts by weight of yeast extract 8 parts by weight, 2 to 6 parts by weight of soyabean lecithin, 0.2 to 0.7 parts by weight of minerals, 0.2 to 0.7 parts by weight of vitamins, and 20 to 60 parts by weight of sea mud preparing a mixture; and
A method for producing a feed composition for aquaculture of sea cucumbers for the production of sea cucumbers with enhanced asthma improvement effect comprising the step of drying and solidifying the mixture.

Images