CN106615638A - Method for reducing fat deposition in livers of Oreochromis niloticus during high-density cultivation - Google Patents
Method for reducing fat deposition in livers of Oreochromis niloticus during high-density cultivation Download PDFInfo
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- CN106615638A CN106615638A CN201610822175.XA CN201610822175A CN106615638A CN 106615638 A CN106615638 A CN 106615638A CN 201610822175 A CN201610822175 A CN 201610822175A CN 106615638 A CN106615638 A CN 106615638A
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- chinese herb
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a method for reducing fat deposition in the livers of Oreochromis niloticus during high-density cultivation. The method comprises the following steps: preparation of a basic ration; preparation of a compound Chinese herbal medicine; addition of the compound Chinese herbal medicine; and feeding of fries. Compared with the prior art, the invention has the following advantages: (1) the method provided by the invention can effectively improve the absorption and utilization of fat in feeds, reduce fat deposition in livers, lower the incidence rate of fatty livers and promote aquaculture of healthy Oreochromis niloticus; (2) the compound Chinese herbal medicine used in the method is a green and environment-friendly preparation, is small in residues in Oreochromis niloticus and low in negative effect on environment, and conforms to requirements on green and environmental-friendly feed additives; and (3) with a compound Chinese herbal medicine feed used in the invention, the absorption efficiency of Oreochromis niloticus is good, no residue is left in the body of Oreochromis niloticus, and excrement of the Oreochromis niloticus is free of pollution to environment.
Description
Technical field
The invention belongs to aquaculture field, is related to aquaculture management and feedstuff feeding technology, and in particular to one kind reduces lucky
The method of Liver fatty deposition in rich Tilapia mossambica high-density breeding.
Background technology
High-density breeding not only can increase water body utilization rate, improve culture benefit and economic benefit, and can be conveniently
Management, reduces labour cost.
In recent years, with the lifting of Tilapia mossambica international status, its yield increases year by year.It is each that Tilapia mossambica has become south China
Save such as Guangdong, Guangxi, the main breed variety in Hainan area.Under current cultivation pattern, high density intensive culture into
For main cultivation trend.However, under high-density breeding, easily cause the non-parasitics fish disease of fish, especially for reduction feed
Cost, feeds for a long time low albumen, and higher fatty acid, the feed of high-carbohydrate causes the metabolic regulation tissue damage such as Tilapia mossambica liver, kidney, heart,
Cause hepatocellular fat deposition.
It is often extensive and comprehensive once there is fatty liver in Tilapia mossambica intensive culture.Its main harm
The fingerling of high-density breeding, adult fish, it is especially ripe individual even more serious.Fatty liver affect Growth Op Tilapia, meat with it is disease-resistant
Power, causes communicable disease and other syndromes when serious, to production huge loss is caused, and dampens the enthusiasm of raiser.
Therefore, gift tilapia Liver fatty deposition under high-density breeding how is reduced, is increased feed fat and is absorbed and utilize, so as to reach
Become our current problems in the urgent need to address to the purpose for promoting gift tilapia healthy aquaculture.
The content of the invention
The technical problem of solution：In order to overcome the defect of prior art, strengthen under gift tilapia high-density breeding for
The absorption of feed fat and Utilization ability, reduce the deposition of liver fat, the invention provides a kind of gift tilapia that improves exists
The feed formulation and feeding method of Liver fatty deposition are reduced during high-density breeding, the absorption of feed fat can be effectively improved
With utilization, Liver fatty deposition is reduced, reduce fatty liver occurrence probability.Meanwhile, the compound Chinese herb formula for being used belongs to green
Color environment-friendly type preparation, fish body is remained and less to the negative interaction of environment, meets wanting for environmental protection friendly feed addictive
Ask.
Technical scheme：A kind of method for reducing Liver fatty deposition in gift tilapia high-density breeding, comprising following step
Suddenly：
1st step, basal diet are prepared：In gift tilapia juvenile fish to adult fish culture stage, it is 28% to prepare protein level,
Fat level is 6% basal diet feed；
2nd step, compound Chinese herb are prepared：By weight, orange peel 25g, burdock are included in every 100g compound Chinese herbs
15g, chrysanthemum 15g, thyme 20g, loguat leaf 25g；
3rd step, compound Chinese herb addition：By weight, per 100g basal diets in add respectively 0.3g～0.4g,
The compound Chinese herb of 0.5g～0.7g, is configured to 0.3%～0.4%, 0.5%～0.7% compound Chinese herb addition group；
4th step, seed feed：When cultivation density is 2500～4000 tails/mu, body size is 50g～150g, feeds and adds
Plus 0.3%～feed of 0.4% compound Chinese herb, body size is more than 150g, feeds 0.5%～0.7% compound Chinese herb
Feed；When cultivation density is higher than 4000 tails/mu, the feed of 0.5%～0.7% compound Chinese herb is fed.
Preferably, in the 2nd step, the compound Chinese herb is obtained by following steps：It is freeze-dried, grind after cross 80
Mesh mesh screen, 4 DEG C of preservations.
Preferably, in the 3rd step, by weight, add the compound Chinese herb of 0.6g in every 100g basal diets respectively, match somebody with somebody
Make 0.6% compound Chinese herb addition group.
Preferably, in the 4th step, cultivation density is 4500 tails/mu, feeds the feed of 0.6% compound Chinese herb.
Preferably, in the 4th step, the time for feeding is：Feed daily twice, respectively 7:00 and 16:00, feeding volume is fish
The 3%～6% of body weight, every 10d water 1/3 is changed, and keeps pond dissolved oxygen sufficient, and water quality is good, and rainy weather is reduced and feeds number of times
With feeding volume, or do not feed.
Beneficial effect：(1) the method for the invention can effectively improve the absorption of feed fat and utilize, and reduce liver
Fat deposition, reduces fatty liver occurrence probability, promotes gift tilapia healthy aquaculture；(2) the compound Chinese herb formula for being used
Belong to environmental type preparation, fish body is remained and less to the negative interaction of environment, meet the addition of environmental protection friendly feed
The requirement of agent；(3) compound Chinese herb feed of the present invention, fish body absorption efficiency is preferable, muscle noresidue, the excreta of fish body
Environmentally safe.
Specific embodiment
Following examples further illustrate present disclosure, but should not be construed as limiting the invention.Without departing substantially from
In the case of spirit of the invention and essence, the modification made to the inventive method, step or condition and replacement belong to the present invention
Scope.If not specializing, the conventional meanses that technological means used is well known to those skilled in the art in embodiment.
Embodiment 1
A kind of method for reducing Liver fatty deposition in gift tilapia high-density breeding, comprises the steps of：
1st step, basal diet are prepared：In gift tilapia juvenile fish to adult fish culture stage, it is 28% to prepare protein level,
Fat level is 6% basal diet feed；
2nd step, compound Chinese herb are prepared：By weight, orange peel 25g, burdock are included in every 100g compound Chinese herbs
15g, chrysanthemum 15g, thyme 20g, loguat leaf 25g；It is freeze-dried, grind after cross 80 mesh mesh screens, 4 DEG C preservation；
3rd step, compound Chinese herb addition：By weight, per 100g basal diets in add respectively 0.3g～0.4g,
The compound Chinese herb of 0.5g～0.7g, is configured to 0.3%～0.4%, 0.5%～0.7% compound Chinese herb addition group；
4th step, seed feed：When cultivation density is 2500～4000 tails/mu, body size is 50g～150g, feeds and adds
Plus 0.3%～feed of 0.4% compound Chinese herb, body size is more than 150g, feeds 0.5%～0.7% compound Chinese herb
Feed；When cultivation density is higher than 4000 tails/mu, the feed of 0.5%～0.7% compound Chinese herb is fed；
The time for feeding is：Feed daily twice, respectively 7:00 and 16:00, feeding volume is the 3%～6% of fish body weight,
Water 1/3 is changed every 10d, keeps pond dissolved oxygen sufficient, water quality is good, rainy weather is reduced and feeds number of times and feeding volume, or not
Feed.
Additionally, setting is not added with the basal diet of compound Chinese herb as a control group, concrete outcome is as shown in 1～table of table 4：
The body size of table 1 is 50g～150g, and cultivation density is 4000 tails/mu
| Control group | 0.3% compound Chinese herb | 0.4% compound Chinese herb | |
| Original body mass (g) | 48.97 | 47.62 | 48.35 |
| Whole opisthosoma weight (g) | 297.53 | 304.22 | 301.81 |
| Liver weight (g) | 8.15 | 6.72 | 7.04 |
| Triglycerides (mmol/L) | 4.53 | 3.27 | 2.45 |
| Cholesterol (mmol/L) | 3.78 | 2.68 | 2.65 |
The body size of table 2 is 50g～150g, and cultivation density is 2500 tails/mu
| Control group | 0.3% compound Chinese herb | 0.4% compound Chinese herb | |
| Original body mass (g) | 32.58 | 31.54 | 31.22 |
| Whole opisthosoma weight (g) | 345.67 | 378.22 | 384.19 |
| Liver weight (g) | 10.43 | 7.45 | 7.83 |
| Triglycerides (mmol/L) | 4.36 | 3.17 | 3.35 |
| Cholesterol (mmol/L) | 4.16 | 3.78 | 3.46 |
The body size of table 3 is more than 150g, and cultivation density is 4000 tails/mu
The body size of table 4 is more than 150g, and cultivation density is 2500 tails/mu
From table 1～4, two kinds of different size Tilapia mossambicas are cultivated in the lab respectively after 60d, Chinese herbal medicine addition group liver
Dirty weight is decreased obviously relative to control group, meanwhile, triglycerides is significantly reduced with cholesterol level in liver.Adult fish culture
In the stage, under high-density breeding, the liver of 6% compound Chinese herb group weight, triglycerides and cholesterol are significantly lower than control group.
Embodiment 2
A kind of method for reducing Liver fatty deposition in gift tilapia high-density breeding, comprises the steps of：
1st step, basal diet are prepared：In gift tilapia juvenile fish to adult fish culture stage, it is 28% to prepare protein level,
Fat level is 6% basal diet feed；
2nd step, compound Chinese herb are prepared：By weight, orange peel 25g, burdock are included in every 100g compound Chinese herbs
15g, chrysanthemum 15g, thyme 20g, loguat leaf 25g；It is freeze-dried, grind after cross 80 mesh mesh screens, 4 DEG C preservation；
3rd step, compound Chinese herb addition：By weight, in adding the compound of 0.6g in every 100g basal diets respectively
Herbal medicine, is configured to 0.6% compound Chinese herb addition group；
4th step, seed feed：Cultivation density is 4500 tails/mu, feeds the feed of 0.6% compound Chinese herb；
The time for feeding is：Feed daily twice, respectively 7:00 and 16:00, feeding volume is the 3%～6% of fish body weight,
Water 1/3 is changed every 10d, keeps pond dissolved oxygen sufficient, water quality is good, rainy weather is reduced and feeds number of times and feeding volume, or not
Feed.
The culture experiment of the present embodiment is carried out respectively in Haikou with Period In Maoming.
Additionally, setting is not added with the basal diet of compound Chinese herb as a control group, concrete outcome is as shown in table 5～8：
Fish body physical sign parameters after the Haikou of table 5 cultivation 100d
| Control group | Compound Chinese herb group | |
| Original body mass (g) | 43.67 | 42.82 |
| Whole opisthosoma weight (g) | 429.78 | 449.21 |
| Liver weight (g) | 11.74 | 8.33 |
As seen from Table 5, cultivate 100d after, although control group go up again in whole opisthosoma with compound Chinese herb addition group it is not poor
It is different, but, the liver of Chinese herbal medicine addition group is decreased obviously again.
Liver biochemical indexes after the Haikou 100d of table 6
| Control group | Compound Chinese herb group | |
| Glutamic-pyruvic transaminase (U.L-1) | 14.11 | 10.63 |
| Glutamic-oxalacetic transaminease (U.L-1) | 69.65 | 56.35 |
| Hepatic glycogen (mmol/L) | 4.49 | 3.82 |
| Triglycerides (mmol/L) | 5.62 | 3.81 |
| Cholesterol (mmol/L) | 2.43 | 1.82 |
As seen from Table 6, cultivate after 100d, liver tg and the cholesterol level of compound Chinese herb group are decreased obviously,
Stress damage of the liver of reduction under high-density breeding, reduces the content of liver glutamic-pyruvic transaminase and glutamic-oxalacetic transaminease.Together
When, liver glycogen content is also decreased obviously.
Fish body physical sign parameters after the Period In Maoming of table 7 cultivation 60d
| Control group | Compound Chinese herb group | |
| Original body mass (g) | 21.62 | 22.43 |
| Whole opisthosoma weight (g) | 293.72 | 306.53 |
| Liver weight (g) | 7.11 | 5.63 |
As seen from Table 7, cultivate after 60d, the liver of the gift tilapia of 0.6% compound Chinese herb addition group is again significantly lower than right
According to group.
Liver biochemical indexes after the Period In Maoming of table 8 cultivation 60d
| Control group | Compound Chinese herb group | |
| Glutamic-pyruvic transaminase (U.L-1) | 16.27 | 14.62 |
| Glutamic-oxalacetic transaminease (U.L-1) | 87.53 | 62.47 |
| Hepatic glycogen (mmol/L) | 5.92 | 4.66 |
| Triglycerides (mmol/L) | 4.73 | 3.21 |
| Cholesterol (mmol/L) | 3.54 | 2.16 |
As seen from Table 8, the hepatic glycogen of the glutamic-oxalacetic transaminease vigor of compound Chinese herb group and liver, triglycerides and cholesterol
Content is significantly lower than control group.
In sum, through present invention process, gift tilapia can substantially drop under 4500 tails/mu high-density breeding
Low liver glycogen and triglycerides and the content of cholesterol, reduce liver weight.Effectively alleviate what is brought due to high-density breeding
The illnesss such as Liver fatty deposition, it is ensured that gift tilapia cultivation efficiency and benefit under high-density breeding, improve its immunological stress energy
Power.
Claims (5)
1. it is a kind of reduce gift tilapia high-density breeding in Liver fatty deposition method, it is characterised in that comprising following step
Suddenly：
1st step, basal diet are prepared：In gift tilapia juvenile fish to adult fish culture stage, it is 28% to prepare protein level, fat
Level is 6% basal diet feed；
2nd step, compound Chinese herb are prepared：By weight, orange peel 25g, burdock 15g are included in every 100g compound Chinese herbs,
Chrysanthemum 15g, thyme 20g, loguat leaf 25g；
3rd step, compound Chinese herb addition：By weight, per 100g basal diets in add respectively 0.3g～0.4g, 0.5g～
The compound Chinese herb of 0.7g, is configured to 0.3%～0.4%, 0.5%～0.7% compound Chinese herb addition group；
4th step, seed feed：When cultivation density is 2500～4000 tails/mu, body size is 50g～150g, feeds addition
The feed of 0.3%～0.4% compound Chinese herb, body size is more than 150g, feeds 0.5%～0.7% compound Chinese herb
Feed；When cultivation density is higher than 4000 tails/mu, the feed of 0.5%～0.7% compound Chinese herb is fed.
2. it is according to claim 1 it is a kind of reduce gift tilapia high-density breeding in Liver fatty deposition method, its
It is characterised by, in the 2nd step, the compound Chinese herb is obtained by following steps：It is freeze-dried, grind after cross 80 mesh nets
Sieve, 4 DEG C of preservations.
3. it is according to claim 1 it is a kind of reduce gift tilapia high-density breeding in Liver fatty deposition method, its
It is characterised by, in the 3rd step, by weight, adds the compound Chinese herb of 0.6g in every 100g basal diets respectively, is configured to
0.6% compound Chinese herb addition group.
4. it is according to claim 1 it is a kind of reduce gift tilapia high-density breeding in Liver fatty deposition method, its
It is characterised by, in the 4th step, cultivation density is 4500 tails/mu, feeds the feed of 0.6% compound Chinese herb.
5. in a kind of reduction gift tilapia high-density breeding according to claim 1 or 4 Liver fatty deposition method,
Characterized in that, in the 4th step, the time for feeding is：Feed daily twice, respectively 7:00 and 16:00, feeding volume is fish body weight
3%～6%, change water 1/3 every 10d, keep pond dissolved oxygen sufficient, water quality is good, and rainy weather reduces and feeds number of times and throw
Amount is fed, or is not fed.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109362613A (en) * | 2018-10-31 | 2019-02-22 | 山东省淡水渔业研究院（山东省淡水渔业监测中心） | The method for propagating low fat crucian artificially |
| CN110559365A (en) * | 2019-09-19 | 2019-12-13 | 中国水产科学研究院淡水渔业研究中心 | Compound Chinese herbal medicine, feed and feed feeding method for promoting fat utilization in circulating water culture of tilapia flume |
| CN111374074A (en) * | 2020-05-09 | 2020-07-07 | 新希望六和股份有限公司 | Nutrition method for regulating and controlling healthy development of liver of micropterus salmoides |
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| CN101889664A (en) * | 2010-04-22 | 2010-11-24 | 广东海洋大学 | Chinese herb medicinal additive for preventing tilapia fatty liver disease |
| WO2011095940A1 (en) * | 2010-02-04 | 2011-08-11 | Willem Adriaan Vermeulen | Feeding composition comprising lemon oil |
| CN104547234A (en) * | 2014-12-19 | 2015-04-29 | 张连 | Traditional Chinese medicine composition for preventing and treating fish liver-gall syndrome |
| CN104664170A (en) * | 2015-03-20 | 2015-06-03 | 广东海大集团股份有限公司 | Feed additive for preventing fatty liver of largemouth bass |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011095940A1 (en) * | 2010-02-04 | 2011-08-11 | Willem Adriaan Vermeulen | Feeding composition comprising lemon oil |
| CN101889664A (en) * | 2010-04-22 | 2010-11-24 | 广东海洋大学 | Chinese herb medicinal additive for preventing tilapia fatty liver disease |
| CN104547234A (en) * | 2014-12-19 | 2015-04-29 | 张连 | Traditional Chinese medicine composition for preventing and treating fish liver-gall syndrome |
| CN104664170A (en) * | 2015-03-20 | 2015-06-03 | 广东海大集团股份有限公司 | Feed additive for preventing fatty liver of largemouth bass |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109362613A (en) * | 2018-10-31 | 2019-02-22 | 山东省淡水渔业研究院（山东省淡水渔业监测中心） | The method for propagating low fat crucian artificially |
| CN110559365A (en) * | 2019-09-19 | 2019-12-13 | 中国水产科学研究院淡水渔业研究中心 | Compound Chinese herbal medicine, feed and feed feeding method for promoting fat utilization in circulating water culture of tilapia flume |
| CN110559365B (en) * | 2019-09-19 | 2021-09-03 | 中国水产科学研究院淡水渔业研究中心 | Compound Chinese herbal medicine, feed and feed feeding method for promoting fat utilization in circulating water culture of tilapia flume |
| CN111374074A (en) * | 2020-05-09 | 2020-07-07 | 新希望六和股份有限公司 | Nutrition method for regulating and controlling healthy development of liver of micropterus salmoides |
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| CN106615638B (en) | 2019-11-22 |
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Effective date of registration: 20200422 Address after: 214000 No.111, Gaoyun Road, Binhu Economic Development Zone, Wuxi City, Jiangsu Province Patentee after: Jiangsu Hengtai Environmental Protection Technology Development Co., Ltd Address before: 214081, No. 9, Shanshui East Road, Binhu District, Jiangsu, Wuxi Patentee before: FRESHWATER FISHERIES RESEARCH CENTER OF CHINESE ACADEMY OF FISHERY SCIENCES |