CN110559102A - Rat non-exposure type tracheal instillation method - Google Patents
Rat non-exposure type tracheal instillation method Download PDFInfo
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- CN110559102A CN110559102A CN201910818094.6A CN201910818094A CN110559102A CN 110559102 A CN110559102 A CN 110559102A CN 201910818094 A CN201910818094 A CN 201910818094A CN 110559102 A CN110559102 A CN 110559102A
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Abstract
The rat non-exposure type tracheal instillation method comprises the following steps: 1, manufacturing a cannula; 2, anaesthetizing; 3, fixing; 4, trachea intubation; 5, instillation; 6, injecting gas; and 7, recovering. In the rat non-exposure type tracheal instillation method provided by the invention, the tracheal intubation operation is simple and easy to operate, the success rate is high, the requirements on instruments and equipment are low, expensive equipment is not required, the probability of injury of the tested rat in the tracheal intubation and instillation process is low, and the rat non-exposure type tracheal instillation method can be widely applied to respiratory tract administration or contamination tests and the establishment of various disease models.
Description
Technical Field
The invention relates to the field of respiratory system disease experimental research, in particular to a rat non-exposure type tracheal instillation method.
Background
The rat tracheal instillation technology is commonly used for rat tracheal administration, rat tracheal administration modeling, rat lung function determination and experimental research related to respiratory diseases. The trachea instillation is carried out during rat related model replication and rat lung function detection, the trachea instillation technology directly influences the success rate of kinetic model replication and success or failure of lung function detection, and the method is very critical in the whole experimental process.
Tracheal instillation is a method of delivering drugs into the lungs through a tracheal tube, which does not require exposure of the trachea through a surgical incision, and is less harmful to the subject being administered, as compared to administration through a tracheal tube. For larger animals (e.g., rabbits or dogs), the tracheal instillation procedure can be accomplished using a standard pediatric laryngoscope. However, for rats, due to the special anatomical conditions of oral cavity and pharynx, no standard intubation device is available for rats at present, instruments used by other species need to be adjusted generally, professional training is needed to perform manual operation, the actual operation difficulty is high, and the intubation success rate is difficult to guarantee.
At present, the following methods are mainly used for evaluating whether rat trachea intubation is successful: 1. placing a mirror at the front end of the intubation tube, and checking whether fog is formed on the mirror when the rat exhales; 2. connecting the cannula to a rodent lung ventilator and observing whether the thoracic cage of the rat can fluctuate regularly with the mechanical ventilation; 3. dripping a drop of water into an extension tube (a vein indwelling needle hose) and connecting the extension tube with the tail end of the cannula, and observing whether the water in the extension tube synchronously vibrates along with the respiration of the rat; 4. constructing an automatic video drip chamber (AVI) to visually evaluate whether the intubation is successful; 5. a rat cilium is fixed at the intubation port in an adhering mode, and whether the rat cilium swings along with the breathing of the rat is observed.
The above methods have the following disadvantages: in method 1, the mist on the mirror generally dissipates faster, making the method unreliable; in the method 2, the rodent lung breathing machine is expensive, and most laboratories do not have the equipment, so that the method is difficult to popularize; in the method 3, the operation steps are more complicated, and the time required for evaluation is longer compared with other methods; in the method 4, expensive hardware equipment is required for supporting the construction of an automatic video drip chamber (AVI), and corresponding equipment is not available in most laboratories, so that the method is difficult to popularize; in the method 5, the inner diameter of the intubation tube of the rat is small, the ventilation volume of the rat during respiration is small, the swinging amplitude of cilia of the rat is small, and the cilia are difficult to observe by naked eyes, so that the method is unreliable.
in conclusion, the research of the rat trachea instillation method which is simple and convenient to operate, high in success rate and low in requirement on expensive equipment and the rat trachea cannula success or failure evaluation method which is simple and convenient to operate, good in reliability and low in requirement on expensive equipment have very important significance.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a rat non-exposure type tracheal instillation method. The device solves the problems that in the existing rat tracheal instillation operation, the difficulty of tracheal intubation is high, the intubation success rate is low, and whether the tracheal intubation is successful or not is difficult to judge.
The technical scheme of the invention is as follows: the rat non-exposure type tracheal instillation method comprises the following steps:
S01, manufacturing a cannula: selecting a No. 9 disposable sterile syringe needle, grinding the tip of the No. 9 disposable sterile syringe needle to be round and blunt, bending the needle at a position 10mm away from one end of the needle ground to be round and blunt, wherein the bending angle a is 14-16 degrees, and the needle is named as a rat trachea cannula, one end of the rat trachea cannula is a liquid outlet end, and the other end of the rat trachea cannula is a syringe connecting end;
S02, anesthesia: selecting adult rats, anesthetizing the adult rats by using diethyl ether, and entering the next step by using the adult rats with relaxed muscles, disappearance of eyelid closing reflex and no contraction reaction of pinching skin or toes by using toothed forceps;
S03, fixing: placing the anesthetized rat on an operating table to a supine position, and fixing incisors on the rat on the operating table by rubber bands and four limbs by cotton ropes;
s04, endotracheal intubation: aligning a light source of a transmission flashlight to the lower jaw of a tested rat, wrapping the tip end of a tissue forceps by a medical adhesive tape, slightly pulling the tongue of the tested rat out of the oral cavity towards one side of the corner of the mouth by using the tissue forceps, adjusting the light angle of the transmission flashlight to enable the laryngeal and laryngeal pipelines of the throat of the tested rat to be visible, then wiping off secretion in the throat of the tested rat by using a sterile cotton swab, extending the liquid outlet end of a tracheal cannula of the rat into the oral cavity from the position between the upper incisor and the lower incisor of the tested rat, and slightly pushing the liquid outlet end of the tracheal cannula of the rat towards the fornix of the hard palate along the curve of the oral cavity so as to avoid damaging the oral cavity and the throat of the rat, so that the tracheal cannula of the rat is inserted into the trachea of the rat, wherein the insertion depth is based on the tracheal cannula;
S05, instillation: sucking 0.6-1.2 ml of infusion solution by using a disposable sterile syringe, then connecting the infusion solution to the needle head connecting end of the rat trachea cannula, and slowly injecting the infusion solution at a constant speed so that the infusion solution gradually drops into the rat trachea;
s06, gas injection: after instillation is completed, taking down the disposable sterile syringe, extracting 0.4-0.6 ml of air by using another disposable sterile syringe, then connecting the disposable sterile syringe to the needle head connecting end of the rat trachea cannula, and injecting the air rapidly, wherein the injection time is controlled within 1 second, so that the instillation liquid can be better diffused to the deep part of the trachea;
S07, resuscitation: and (3) pulling out the rat trachea cannula within 5 seconds after the gas injection, holding the tail of the tested rat, hanging the rat for 4-6 seconds, and then putting down the rat until the anesthesia effect disappears.
the further technical scheme of the invention is as follows: in step S01, the bending angle a is 15 °.
The further technical scheme of the invention is as follows: in the step S03, the operating table is tilted 74 to 76 ° with respect to the horizontal plane, and the head of the rat is upward after the rat is fixed.
the invention further adopts the technical scheme that: in step S03, the operating table is tilted 75 ° with respect to the horizontal plane.
the invention further adopts the technical scheme that: in step S04, the insertion depth is determined by the remaining 25% of the length of the rat' S trachea cannula outside the upper incisors of the rat.
The further technical scheme of the invention is as follows: in step S04, during the process of inserting the rat tracheal cannula into the rat trachea, the thickened respiratory sound is heard through the stethoscope, which indicates the success of the tracheal cannula.
The further technical scheme of the invention is as follows: and S04, in the process of inserting the rat trachea cannula into the rat trachea, if thickened breath sounds are not heard through the stethoscope and the rat trachea cannula is mistakenly inserted into the rat esophagus, performing a second intubation operation, namely, extending the liquid outlet end of the second rat trachea cannula into the oral cavity from the position between the upper incisor and the lower incisor of the tested rat, slightly advancing the liquid outlet end of the second rat trachea cannula along the curve of the oral cavity and towards the fornix of the hard palate, so that the second rat trachea cannula is inserted into the rat trachea, and finally, pulling out the rat trachea cannula mistakenly inserted into the rat esophagus.
the further technical scheme of the invention is as follows: in step S05, the amount of the instillation solution was 1.0ml by aspiration with a disposable sterile syringe.
The further technical scheme of the invention is as follows: in step S06, another disposable sterile syringe was used to draw 0.5ml of air.
Compared with the prior art, the invention has the following advantages:
1. In the rat non-exposure type tracheal instillation method provided by the invention, the tracheal intubation operation is simple and easy to operate, the success rate is high, the requirements on instruments and equipment are low, expensive equipment is not required, the probability of injury of the tested rat in the tracheal intubation and instillation process is low, and the rat non-exposure type tracheal instillation method can be widely applied to respiratory tract administration or contamination tests and the establishment of various disease models.
2. In the rat non-exposure type tracheal instillation method provided by the invention, the method for judging whether the tracheal intubation succeeds is simple, rapid and good in reliability, expensive equipment is not required, and good guarantee is provided for smooth implementation of tracheal instillation.
The invention is further described below with reference to the figures and examples.
Drawings
FIG. 1 is a schematic structural view of a rat trachea cannula;
FIG. 2 is a graph showing the observation result of pathological examination of rat lung tissues in a specific application example of the present invention.
Detailed Description
Example 1:
the rat non-exposure type tracheal instillation method comprises the following steps:
s01, manufacturing a cannula: a No. 9 disposable sterile syringe needle is selected, the tip of the No. 9 disposable sterile syringe needle is ground to be round and blunt, the needle is bent at a position 10mm away from one end of the needle ground to be round and blunt, the bending angle a is 14-16 degrees, the needle is named as a rat trachea cannula (see figure 1), one end of the rat trachea cannula 1 is a liquid outlet end 11, and the other end of the rat trachea cannula is a syringe connecting end 12.
s02, anesthesia: adult rats were selected and anesthetized with ether, and the next step was performed with muscle relaxation, disappearance of eyelid closure reflex, no contraction response of skin or toes with nipper with dental forceps.
S03, fixing: the anesthetized rat is placed on an operating table to be in a supine position, and the incisors on the rat are fixed on the operating table through rubber bands and cotton ropes for four limbs.
S04, endotracheal intubation: aligning a light source of a transmission flashlight to the lower jaw of a tested rat, wrapping the tip end of a tissue forceps by a medical adhesive tape, slightly pulling the tongue of the tested rat out of the oral cavity towards one side of the corner of the mouth by using the tissue forceps, stopping pulling when resistance is sensed, adjusting the light angle of the transmission flashlight to enable the throat pipeline of the tested rat to be visible, then wiping off secretion in the throat of the tested rat by using a sterile cotton swab, extending the liquid outlet end of a tracheal cannula of the rat into the oral cavity from the position between the upper incisor and the lower incisor of the tested rat, slightly pushing the tracheal cannula towards the fornix of the hard palate along the curve of the oral cavity, slightly lifting the tracheal cannula of the rat when the tracheal cannula of the rat touches the root of the tongue of the rat to avoid damaging the oral cavity and the throat of the rat, and inserting the tracheal cannula of the rat into the trachea, wherein the insertion depth is based on the tracheal cannula of the rat with the length of 20-30%.
S05, instillation: 0.6-1.2 ml of instillation liquid is absorbed by a disposable sterile syringe, then the instillation liquid is connected to the needle head connecting end of the rat trachea cannula, and then the instillation liquid is injected slowly and at a constant speed, so that the instillation liquid gradually enters the rat trachea.
S06, gas injection: after the instillation is completed, the disposable sterile syringe is taken down, 0.4-0.6 ml of air is extracted by the other disposable sterile syringe, then the needle head connecting end of the rat trachea cannula is connected, the injection is carried out rapidly, the injection time is controlled in 1 second, and the instillation liquid is better diffused to the deep part of the trachea.
s07, resuscitation: and (3) pulling out the rat trachea cannula within 5 seconds after the gas injection, holding the tail of the tested rat, hanging the rat for 4-6 seconds, and then putting down the rat until the anesthesia effect disappears.
Preferably, in the step S01, the bending angle a is 15 °.
Preferably, in step S03, the operating table is tilted 75 ° with respect to the horizontal plane, and the subject rat is fixed with its head facing upward.
Preferably, in step S04, the insertion depth is based on the remaining 25% of the length of the rat' S trachea cannula outside the rat incisors.
preferably, in step S04, during the rat tracheal intubation inserting into the rat trachea, the thickened respiratory sound is heard through the stethoscope, i.e. it indicates successful tracheal intubation.
preferably, in step S04, if the thickened breath sound is not heard by the stethoscope during the rat tracheal intubation is inserted into the rat trachea, indicating that the rat tracheal intubation is inserted into the rat esophagus by mistake, a second intubation operation is performed, that is, the liquid outlet end of the second rat tracheal intubation extends into the oral cavity from between the upper incisor and the lower incisor of the tested rat, and is gently pushed along the curve of the oral cavity toward the fornix of the hard palate, so that the second rat tracheal intubation is inserted into the rat trachea, and finally the rat tracheal intubation inserted into the rat esophagus by mistake is pulled out.
Preferably, in the step S05, the amount of the instillation solution sucked up by a disposable sterile syringe is 1.0 ml.
Preferably, in the step of S06, the amount of air drawn by the other disposable sterile syringe is 0.5 ml.
Briefly describing the application of the invention: selecting 10 adult rats, carrying out tracheal instillation by adopting the rat non-exposure type tracheal instillation method provided by the invention, wherein the instillation liquid is sterilized ultrapure water, and after instillation is finished, 10 rats do not die or have any respiratory problem and can be quickly recovered to a normal state, and the success rate of tracheal instillation is 100%.
The instillation was completed by routine histopathological examination of rat lung tissue and was found to be: the rat lung tissue morphology was normal, the alveolar and alveolar epithelium was intact and clear, the alveolar space was normal in size, and there was no inflammatory cell infiltration around the alveolar space (see fig. 2).
Compared with the prior art, the rat non-exposure type tracheal instillation method provided by the invention has the advantages that: 1. the required device has low cost and simple and easy manufacture; 2. the operation is simple, the popularization is convenient, and the operation can be mastered after simple knowledge or demonstration; 3. the time consumption is short, the whole tracheal instillation process (a single rat) only needs about 2min, and after the instillation is finished, the rat can wake up quickly without influencing normal physiological activity; 4. the method for judging whether the tracheal intubation succeeds is simple and reliable, can be synchronously performed with the tracheal intubation, namely instillation operation, does not consume redundant time, immediately performs the second tracheal intubation when the first tracheal intubation does not enter the trachea, extracts the first tracheal intubation after the second tracheal intubation succeeds, can succeed by performing the second tracheal intubation at most, and avoids the rat from being damaged due to repeated intubation.
Claims (9)
1. The rat non-exposure type tracheal instillation method is characterized in that: the method comprises the following steps:
S01, manufacturing a cannula: selecting a No. 9 disposable sterile syringe needle, grinding the tip of the No. 9 disposable sterile syringe needle to be round and blunt, bending the needle at a position 10mm away from one end of the needle ground to be round and blunt, wherein the bending angle a is 14-16 degrees, and the needle is named as a rat trachea cannula, one end of the rat trachea cannula is a liquid outlet end, and the other end of the rat trachea cannula is a syringe connecting end;
s02, anesthesia: selecting adult rats, anesthetizing the adult rats by using diethyl ether, and entering the next step by using the adult rats with relaxed muscles, disappearance of eyelid closing reflex and no contraction reaction of pinching skin or toes by using toothed forceps;
s03, fixing: placing the anesthetized rat on an operating table to a supine position, and fixing incisors on the rat on the operating table by rubber bands and four limbs by cotton ropes;
s04, endotracheal intubation: aligning a light source of a transmission flashlight to the lower jaw of a tested rat, wrapping the tip end of a tissue forceps by a medical adhesive tape, slightly pulling the tongue of the tested rat out of the oral cavity towards one side of the corner of the mouth by using the tissue forceps, adjusting the light angle of the transmission flashlight to enable the laryngeal and laryngeal pipelines of the throat of the tested rat to be visible, then wiping off secretion in the throat of the tested rat by using a sterile cotton swab, extending the liquid outlet end of a tracheal cannula of the rat into the oral cavity from the position between the upper incisor and the lower incisor of the tested rat, and slightly pushing the liquid outlet end of the tracheal cannula of the rat towards the fornix of the hard palate along the curve of the oral cavity so as to avoid damaging the oral cavity and the throat of the rat, so that the tracheal cannula of the rat is inserted into the trachea of the rat, wherein the insertion depth is based on the tracheal cannula;
S05, instillation: sucking 0.6-1.2 ml of infusion solution by using a disposable sterile syringe, then connecting the infusion solution to the needle head connecting end of the rat trachea cannula, and slowly injecting the infusion solution at a constant speed so that the infusion solution gradually drops into the rat trachea;
S06, gas injection: after instillation is completed, taking down the disposable sterile syringe, extracting 0.4-0.6 ml of air by using another disposable sterile syringe, then connecting the disposable sterile syringe to the needle head connecting end of the rat trachea cannula, and injecting the air rapidly, wherein the injection time is controlled within 1 second, so that the instillation liquid can be better diffused to the deep part of the trachea;
S07, resuscitation: and (3) pulling out the rat trachea cannula within 5 seconds after the gas injection, holding the tail of the tested rat, hanging the rat for 4-6 seconds, and then putting down the rat until the anesthesia effect disappears.
2. The rat non-exposed tracheal instillation method of claim 1, wherein: in step S01, the bending angle a is 15 °.
3. the rat non-exposed tracheal instillation method of claim 1, wherein: in the step S03, the operating table is tilted 74 to 76 ° with respect to the horizontal plane, and the head of the rat is upward after the rat is fixed.
4. a rat non-exposed tracheal instillation method according to claim 3, wherein: in step S03, the operating table is tilted 75 ° with respect to the horizontal plane.
5. A rat non-exposed tracheal instillation method according to claim 3, wherein: in step S04, the insertion depth is determined by the remaining 25% of the length of the rat' S trachea cannula outside the upper incisors of the rat.
6. The rat non-exposed tracheal instillation method of claim 5, wherein: in step S04, during the process of inserting the rat tracheal cannula into the rat trachea, the thickened respiratory sound is heard through the stethoscope, which indicates the success of the tracheal cannula.
7. The rat non-exposed tracheal instillation method of claim 6, wherein: and S04, in the process of inserting the rat trachea cannula into the rat trachea, if thickened breath sounds are not heard through the stethoscope and the rat trachea cannula is mistakenly inserted into the rat esophagus, performing a second intubation operation, namely, extending the liquid outlet end of the second rat trachea cannula into the oral cavity from the position between the upper incisor and the lower incisor of the tested rat, slightly advancing the liquid outlet end of the second rat trachea cannula along the curve of the oral cavity and towards the fornix of the hard palate, so that the second rat trachea cannula is inserted into the rat trachea, and finally, pulling out the rat trachea cannula mistakenly inserted into the rat esophagus.
8. The rat non-exposed tracheal instillation method of any one of claims 1-7, wherein: in step S05, the amount of the instillation solution was 1.0ml by aspiration with a disposable sterile syringe.
9. The rat non-exposed tracheal instillation method of claim 8, wherein: in step S06, another disposable sterile syringe was used to draw 0.5ml of air.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112998899A (en) * | 2021-02-20 | 2021-06-22 | 中国人民解放军陆军军医大学 | Experimental animal trachea instillator |
| CN116236314A (en) * | 2023-04-26 | 2023-06-09 | 右江民族医学院 | Improved novel noninvasive mouse trachea instillation molding method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104146792A (en) * | 2014-08-04 | 2014-11-19 | 天津中医药大学第二附属医院 | Intratracheal dosing device for rat |
| CN106620998A (en) * | 2016-11-13 | 2017-05-10 | 徐兴祥 | Tracheal intubation needle for mouse and tracheal intubation method for mouse |
| CN106725988A (en) * | 2016-12-01 | 2017-05-31 | 甘肃中医药大学 | A kind of rat trachea intubation procedure and its application |
| CN107736951A (en) * | 2017-10-27 | 2018-02-27 | 北京大学第三医院 | A kind of large and small mouse trachea cannula method |
| CN108542549A (en) * | 2018-05-17 | 2018-09-18 | 宁波大学 | Tracheae epiglottis opening site-specific delivery of drugs method suitable for the noninvasive respiratory tract administration of rat |
| CN109364343A (en) * | 2018-11-13 | 2019-02-22 | 菏泽学院 | A kind of rat Physiological Experiment respiratory tract microstimulation vented cannula |
-
2019
- 2019-08-30 CN CN201910818094.6A patent/CN110559102A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104146792A (en) * | 2014-08-04 | 2014-11-19 | 天津中医药大学第二附属医院 | Intratracheal dosing device for rat |
| CN106620998A (en) * | 2016-11-13 | 2017-05-10 | 徐兴祥 | Tracheal intubation needle for mouse and tracheal intubation method for mouse |
| CN106725988A (en) * | 2016-12-01 | 2017-05-31 | 甘肃中医药大学 | A kind of rat trachea intubation procedure and its application |
| CN107736951A (en) * | 2017-10-27 | 2018-02-27 | 北京大学第三医院 | A kind of large and small mouse trachea cannula method |
| CN108542549A (en) * | 2018-05-17 | 2018-09-18 | 宁波大学 | Tracheae epiglottis opening site-specific delivery of drugs method suitable for the noninvasive respiratory tract administration of rat |
| CN109364343A (en) * | 2018-11-13 | 2019-02-22 | 菏泽学院 | A kind of rat Physiological Experiment respiratory tract microstimulation vented cannula |
Non-Patent Citations (5)
| Title |
|---|
| 冯淬灵等: "清金化痰汤对慢性阻塞性肺疾病模型大鼠肺组织中性粒细胞弹性蛋白酶及黏蛋白基因表达的影响", 《中国中医药信息杂志》 * |
| 张伟等: "肺纤方对博莱霉素致肺间质纤维化大鼠肺系数与肺组织病理的影响", 《北京中医药大学学报》 * |
| 晋乐飞等: "吸入式气管滴注法的建立", 《郑州大学学报(医学版)》 * |
| 杨春燕等: "经呼吸道给药实验应注意的问题", 《中国药理学与毒理学杂志》 * |
| 练毅刚等: "扶正剔邪搜络方对肺纤维化大鼠肺功能的影响", 《中医杂志》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112998899A (en) * | 2021-02-20 | 2021-06-22 | 中国人民解放军陆军军医大学 | Experimental animal trachea instillator |
| CN116236314A (en) * | 2023-04-26 | 2023-06-09 | 右江民族医学院 | Improved novel noninvasive mouse trachea instillation molding method |
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Application publication date: 20191213 |