CN106620998A - Tracheal intubation needle for mouse and tracheal intubation method for mouse - Google Patents
Tracheal intubation needle for mouse and tracheal intubation method for mouse Download PDFInfo
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- CN106620998A CN106620998A CN201610996557.4A CN201610996557A CN106620998A CN 106620998 A CN106620998 A CN 106620998A CN 201610996557 A CN201610996557 A CN 201610996557A CN 106620998 A CN106620998 A CN 106620998A
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- mouse
- trachea cannula
- pintle
- needle
- pin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M25/00—Catheters; Hollow probes
- A61M25/01—Introducing, guiding, advancing, emplacing or holding catheters
- A61M25/0102—Insertion or introduction using an inner stiffening member, e.g. stylet or push-rod
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M16/00—Devices for influencing the respiratory system of patients by gas treatment, e.g. mouth-to-mouth respiration; Tracheal tubes
- A61M16/04—Tracheal tubes
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- Health & Medical Sciences (AREA)
- Pulmonology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Heart & Thoracic Surgery (AREA)
- Anesthesiology (AREA)
- Biomedical Technology (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Emergency Medicine (AREA)
- Biophysics (AREA)
- Surgical Instruments (AREA)
Abstract
The invention relates to a tracheal intubation needle for a mouse and a tracheal intubation method for a mouse. Firstly, the tracheal intubation needle for the mouse is made, normal saline is injected into a transparent pintle on the tracheal intubation needle for the mouse to form visible liquid level in the transparent pintle, then the mouse is anesthetized after being fixed at a supine position, the neck of the mouse is irradiated with a cold lamp source, then the tongue of the mouse is clamped by using a pair of tweezers, outward traction is conducted on the tongue of the mouse, and the lower jaw of the mouse is supported; then, the tracheal intubation needle used for the mouse and forming the liquid level in the transparent pintle is inserted into the trachea, the liquid level at the pintle of the tracheal intubation needle for the mouse vertically fluctuates with the respiratory frequency of the mouse, and if the frequencies of the two parts are consistent, it is indicated that the tracheal intubation needle is correctly inserted into the trachea. The tracheal intubation needle has the advantages that the tracheal intubation needle is simple and ingenious in design and easy to manufacture, and materials are convenient to obtain; the needle is ingenious in principle and utilizes the principle that the liquid level in the closed airway easily and vertically fluctuates with respiration; intubation is performed from the mouth of the mouse without postoperative complications, and the tracheal intubation needle is safe, reliable and high in success rate.
Description
Technical field
The present invention relates to a kind of mouse trachea cannula pin and to mouse trachea cannula method, belongs to medical experiment technology neck
Domain.
Background technology
In the genome times afterwards comprehensively, mouse becomes one of requisite conventional animal in modern medicine test.Mouse is to grind
Study carefully disease mechanism and determine the relatively good tool of the validity that therapeutic scheme, checking new compound and medicine are denoted as, existing
For occupying the status that becomes more and more important in medical research.Trachea cannula is open chest surgery, respiratory function detection, pulmonary administration, animal
Committed step during model and disease research.Tracheae is carried out more than the past researcher using direct oral cavity or operation tracheotomy to insert
Pipe, because the special anatomical structure of murine oral, particularly lower jaw can not be fully opened, it is impossible to which tracheostomy is looked at straight by oral cavity,
Direct oral cavity is set directly to acquire a certain degree of difficulty as trachea cannula, success rate is extremely low, and in order to detect whether to insert tracheal strips, multiplex breathing
Machine detected, this adds increased experiment fees budget.This method of underwent operative tracheostomy tube is although easy to operate, but is
A kind of invasive operation, injures larger to mouse body, the sterility when size of otch, the degree of tissue damage, operation
And it is postoperative whether infect the recovery that can all affect postoperative mouse body, and mouse is difficult to tolerate Repeated Operation, experiment
Poor repeatability, mouse survival rate is low, and only 35% ~ 45%.And some strike DNA rat and particular module mouse is expensive, death is not
Economic loss can be only caused, experiment process is affected, serious sense of defeat can be caused when serious to experimenter, or even body and mind can be caused
Health problem.
The content of the invention
The purpose of the present invention is aiming at above-mentioned problems of the prior art, there is provided a kind of new without the need for special installation
And the mouse trachea cannula pin that can complete of position, one man operation and to mouse trachea cannula method.
The object of the present invention is achieved like this, a kind of mouse trachea cannula pin, including cannula needle, pintle, it is characterized in that,
The front end bending of the cannula needle, forms bend, and the front end bend of cannula needle is cased with protecting mouse tracheae in tracheae
The flexible pipe damaged from cannula needle when cannula needle is inserted；The pintle is nonmetallic transparent pintle.
The pintle is polypropylene pintle.
The flexible pipe is 1-1.5cm sterile blood samplings flexible pipe or Vein Tube flexible pipe for length.
One kind is directed to mouse trachea cannula method using mouse trachea cannula, it is characterized in that, comprises the following steps：
Step（1）, prepare trachea cannula pin；
A, 1 injection needle is taken, the pintle of injection needle is nonmetallic transparent pintle；
B, the needle point of injection needle is cut off, and injection needle front end is pressed from both sides into curved, injection needle front end forms injection needle bend；
C, flexible pipe is taken, and flexible pipe is pulled into the diameter matched with injection needle by the flame of aseptic alcolhol burner, then cut one
The flexible pipe of segment length 1-1.5cm, by the Hose Jacket of 1-1.5cm in the front end of injection needle bend, to protect mouse tracheae in gas
Damage from cannula needle when cannula pin is inserted, complete the preparation of trachea cannula pin；
Step（2）, with syringe to step（1）Saline injection in the transparent pintle on trachea cannula pin for preparing, directly
Form visible liquid to transparent pintle to put down；
Step（3）, will mouse dorsal position fix after anaesthetize, with cold lamp source irradiate mouse neck, then clamp mouse with pincet
Tongue, the tongue of mouse is drawn outward, by mouse lower jaw hold up, looking in from mouse oral area can see mouse epiglottis portion one
There is a bright point Zhang Yihe, centre, that is, the entrance of tracheae；
Step（4）, by step（2）The flat trachea cannula pin of liquid, the oral cavity of Jing mouse, epiglottis, glottis are formed with transparent pintle
Into tracheae, if insertion tracheae, the liquid at trachea cannula pin pintle is flat will up and down to fluctuate with mouse breathing frequency, and the two frequency
Unanimously, that is, represent that trachea cannula pin is correctly inserted into tracheal strips, and animal respirator interface can be connected to the pintle of trachea cannula pin
On, or injection Experimental agents are tested in trachea cannula pin；
If trachea cannula pin is inserted in oesophagus or stomach, liquid is flat at pintle will not up and down fluctuate with mouse respiratory rate, or only light
Microwave is moved, and can be extracted on trachea cannula pin, reinsert mouse tracheae until liquid at trachea cannula pin pintle is flat will be with mouse
Respiratory rate fluctuates up and down, and the two frequency is consistent, and trachea cannula pin is correctly inserted into tracheal strips.
Step（1）In, the pintle is polypropylene pintle.
Step（1）In, the scissors cuts off at the needle point of injection needle.
Step（1）In, the injection needle front end is curved with hemostasis clamp.
Step（1）In, the flexible pipe is sterile blood sampling flexible pipe or Vein Tube flexible pipe.
Step（2）In, the syringe is 5mL syringes.
The advanced science of the inventive method, the beneficial effects of the present invention is：
1st, design simple exquisite, draw materials conveniently, it is easy to make.When making, it is only necessary to which it is nonmetallic transparent pintle to take pintle
Injection needle, then cuts off at the needle point of injection needle, and injection needle front end is pressed from both sides into bending into injection needle bend, then in bend
The flexible pipe of 1-1.5cm is put, flexible pipe can be sterile blood sampling flexible pipe or Vein Tube flexible pipe.Either injection needle or flexible pipe,
These are all very common things, convenient to make conveniently so as to draw materials easily.
2nd, principle is ingenious, and fluctuated up and down the principle being easy to using liquid level in closure air flue with breathing.That is, mouse trachea cannula
Pin, the oral cavity of Jing mouse, epiglottis, glottis enter tracheae, if insertion tracheae, the liquid at mouse trachea cannula pin pintle is flat will be with little
Mouse respiratory rate fluctuates up and down, and the two frequency is consistent, that is, represent that mouse trachea cannula pin is correctly inserted into tracheal strips, can move
Thing lung ventilator interface is connected on the pintle of mouse trachea cannula pin, or injection Experimental agents carry out reality in mouse trachea cannula pin
Test.
3rd, Jing murine orals intubation, safe and reliable without postoperative complications, success rate is high.
Description of the drawings
Fig. 1 is the structural representation of mouse trachea cannula pin of the present invention.
In figure：1 cannula needle, 2 pintles, 3 bends, 4 flexible pipes.
Specific embodiment
With reference to specific embodiment, the present invention is expanded on further.
The present embodiment includes following operation：
First, the making of mouse trachea cannula pin.
1st, No. nine injection needles are taken（The model of pin can be determined according to mouse size）1, but pintle（Injection needle is connected with syringe
Place）Must be nonmetallic materials, and it is transparent, the present embodiment selects polypropylene pintle.
2nd, injection needle needle point is cut off with scissors, and injection needle leading portion is curved with hemostasis clamp, and its crooked radian is adapted to little
The physiological anatomic architecture of mouse bottleneck throat and tracheae.
3rd, one section of sterile blood sampling flexible pipe or Vein Tube flexible pipe are taken, diameter is pulled into by the flame of aseptic alcolhol burner with injection
Pin is correspondingly sized, cuts the flexible pipe of one section of long 1-1.5cm, is enclosed within the leading portion of injection needle bend, to protect mouse trachea cannula
When from metal needle（Mouse trachea cannula pin）Damage.
4th, a small amount of physiological saline is injected in transparent pintle with 5mL syringes so as to form visible liquid and put down, it is stand-by.
2nd, trachea cannula step.
1st, will anaesthetize after the fixation of mouse dorsal position, with cold lamp source mouse neck is irradiated, then right hand pincet clamps tongue
Head draws outward, and left hand holds up lower jaw with special instrument, and looking in from mouse oral area can see the conjunction of one, mouse epiglottis portion,
There is a bright point centre, that is, the entrance of tracheae.
2nd, off-the-shelf mouse trachea cannula pin is taken（Transparent pintle is formed with the flat trachea cannula pin of visible liquid）, orally
Chamber, epiglottis, glottis enter tracheae, if insertion tracheae, the liquid at trachea cannula pin pintle is flat will be with the upper and lower ripple of mouse breathing frequency
It is dynamic, and the two frequency is consistent, and animal respirator interface can be connected on the pintle of mouse trachea cannula pin, or to mouse tracheae
Inject Experimental agents in cannula needle to be tested.If in insertion oesophagus or stomach, liquid is flat at pintle will not be with mouse breathing frequency
Fluctuate up and down, or only slight fluctuations, can extract, again intubation is until success, will mouse trachea cannula pin reinsert it is little
Mouse tracheae will up and down fluctuate up to the liquid at mouse trachea cannula pin pintle is flat with mouse breathing frequency, and the two frequency is consistent,
Mouse trachea cannula pin is correctly inserted into tracheal strips.
3rd, dissect and confirm.
Treating that the liquid at trachea cannula pin pintle is flat will up and down fluctuate with mouse breathing frequency, and the two frequency is consistent, then recognize
For mouse trachea cannula pin insert mouse tracheae success, now, it was demonstrated that if can with syringe Jing mouse trachea cannula pins to
The mouse trachea cannula pin place of inserting injection 2mL methylene blues in mouse, then dissect mouse, by methylene blue in tracheae and lung（It is beautiful
It is blue successfully to inject in tracheae and lung）, this may certify that treat that liquid at trachea cannula pin pintle is flat will be with mouse breathing frequency
Lower fluctuation, and the two frequency is consistent, then mouse trachea cannula pin insertion mouse tracheae success；Such as, liquid is flat at pintle will not be with mouse
Respiratory rate fluctuates up and down, or only slight fluctuations, then it is assumed that mouse trachea cannula pin is not inserted into mouse tracheae, trachea cannula
Pin insertion be in oesophagus or stomach, now, it was demonstrated that if can with syringe Jing mouse trachea cannula pins to the mouse in mouse
2mL methylene blues are injected at the trachea cannula pin place of inserting, and mouse are then dissected, by methylene blue in oesophagus or stomach（Methylene blue injection is food
In pipe or stomach）.
Claims (9)
1. a kind of mouse trachea cannula pin, including cannula needle（1）, pintle（2）, it is characterized in that, the cannula needle（1）Front end it is curved
Song, forms bend（3）, cannula needle（1）Front end bend（3）It is cased with protecting mouse tracheae to insert in trachea cannula pin
When from cannula needle（1）The flexible pipe of damage（4）；The pintle（2）For nonmetallic transparent pintle.
2. a kind of mouse trachea cannula pin according to claim 1, is characterized in that, the pintle（2）For polypropylene pintle.
3. a kind of mouse trachea cannula pin according to claim 1, is characterized in that, the flexible pipe（4）It is 1- for length
1.5cm sterile blood samplings flexible pipe or Vein Tube flexible pipe.
4. it is a kind of to be directed to mouse trachea cannula method using mouse trachea cannula, it is characterized in that, comprise the following steps：
Step（1）, prepare trachea cannula pin；
A, 1 injection needle is taken, the pintle of injection needle is nonmetallic transparent pintle；
B, the needle point of injection needle is cut off, and injection needle front end is pressed from both sides into curved, injection needle front end forms injection needle bend；
C, flexible pipe is taken, and flexible pipe is pulled into the diameter matched with injection needle by the flame of aseptic alcolhol burner, then cut one
The flexible pipe of segment length 1-1.5cm, by the Hose Jacket of 1-1.5cm in the front end of injection needle bend, to protect mouse tracheae in gas
Damage from cannula needle when cannula pin is inserted, complete the preparation of mouse trachea cannula pin；
Step（2）, with syringe to step（1）Physiology salt is injected in the transparent pintle on mouse trachea cannula pin for preparing
Water, until visible liquid is formed in transparent pintle putting down；
Step（3）, will mouse dorsal position fix after anaesthetize, with cold lamp source irradiate mouse neck, then clamp mouse with pincet
Tongue, the tongue of mouse is drawn outward, by mouse lower jaw hold up, looking in from mouse oral area can see mouse epiglottis portion one
There is a bright point Zhang Yihe, centre, that is, the entrance of tracheae；
Step（4）, by step（2）Be formed with the flat mouse trachea cannula pin of liquid in transparent pintle, the oral cavity of Jing mouse, epiglottis,
Glottis enters tracheae, if insertion tracheae, the liquid at mouse trachea cannula pin pintle is flat will up and down to fluctuate with mouse breathing frequency, and
The two frequency is consistent, that is, represent that mouse trachea cannula pin is correctly inserted into tracheal strips, animal respirator interface can be connected into mouse
On the pintle of trachea cannula pin, or injection Experimental agents are tested in mouse trachea cannula pin；
If mouse trachea cannula pin is inserted in oesophagus or stomach, liquid is flat at pintle will not up and down fluctuate with mouse respiratory rate, or only
There are slight fluctuations, mouse trachea cannula pin can be extracted, reinsert mouse tracheae until at mouse trachea cannula pin pintle
Liquid is flat will up and down to fluctuate with mouse breathing frequency, and the two frequency is consistent, and mouse trachea cannula pin is correctly inserted into tracheal strips.
5. one kind according to claim 4 is directed to mouse trachea cannula method using mouse trachea cannula, it is characterized in that, walks
Suddenly（1）In, the pintle is polypropylene pintle.
6. one kind according to claim 4 is directed to mouse trachea cannula method using mouse trachea cannula, it is characterized in that, walks
Suddenly（1）In, the scissors cuts off at the needle point of injection needle.
7. one kind according to claim 4 is directed to mouse trachea cannula method using mouse trachea cannula, it is characterized in that, walks
Suddenly（1）In, the injection needle front end is curved with hemostasis clamp.
8. one kind according to claim 4 is directed to mouse trachea cannula method using mouse trachea cannula, it is characterized in that, walks
Suddenly（1）In, the flexible pipe is sterile blood sampling flexible pipe or Vein Tube flexible pipe.
9. one kind according to claim 4 is directed to mouse trachea cannula method using mouse trachea cannula, it is characterized in that, walks
Suddenly（2）In, the syringe is 5mL syringes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610996557.4A CN106620998A (en) | 2016-11-13 | 2016-11-13 | Tracheal intubation needle for mouse and tracheal intubation method for mouse |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610996557.4A CN106620998A (en) | 2016-11-13 | 2016-11-13 | Tracheal intubation needle for mouse and tracheal intubation method for mouse |
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| CN201610996557.4A Pending CN106620998A (en) | 2016-11-13 | 2016-11-13 | Tracheal intubation needle for mouse and tracheal intubation method for mouse |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109452987A (en) * | 2019-01-03 | 2019-03-12 | 温州医科大学附属第二医院、温州医科大学附属育英儿童医院 | One kind being used for mouse tracheal cannula experimental facility |
| CN110559102A (en) * | 2019-08-30 | 2019-12-13 | 南华大学 | Rat non-exposure type tracheal instillation method |
| CN110624164A (en) * | 2018-06-24 | 2019-12-31 | 吴庆景 | Preparation and use of rat trachea cannula equipment |
| CN112126660A (en) * | 2020-09-28 | 2020-12-25 | 新疆医科大学第一附属医院 | Preparation method of animal model with high expression of gene transfected in lung tissue through intratracheal way |
| CN112998899A (en) * | 2021-02-20 | 2021-06-22 | 中国人民解放军陆军军医大学 | Experimental animal trachea instillator |
| CN113876457A (en) * | 2021-09-23 | 2022-01-04 | 安徽理工大学 | Economical, quantitative and noninvasive method for establishing mouse model for occupational disease of pulmonary fibrosis caused by silicon dust and preparation device |
| CN116712205A (en) * | 2023-06-20 | 2023-09-08 | 中南大学湘雅二医院 | Tool set for preparing acute lung injury mouse model |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110624164A (en) * | 2018-06-24 | 2019-12-31 | 吴庆景 | Preparation and use of rat trachea cannula equipment |
| CN109452987A (en) * | 2019-01-03 | 2019-03-12 | 温州医科大学附属第二医院、温州医科大学附属育英儿童医院 | One kind being used for mouse tracheal cannula experimental facility |
| CN109452987B (en) * | 2019-01-03 | 2020-09-01 | 温州医科大学附属第二医院、温州医科大学附属育英儿童医院 | Be used for mouse trachea cannula experimental apparatus |
| CN110559102A (en) * | 2019-08-30 | 2019-12-13 | 南华大学 | Rat non-exposure type tracheal instillation method |
| CN112126660A (en) * | 2020-09-28 | 2020-12-25 | 新疆医科大学第一附属医院 | Preparation method of animal model with high expression of gene transfected in lung tissue through intratracheal way |
| CN112998899A (en) * | 2021-02-20 | 2021-06-22 | 中国人民解放军陆军军医大学 | Experimental animal trachea instillator |
| CN113876457A (en) * | 2021-09-23 | 2022-01-04 | 安徽理工大学 | Economical, quantitative and noninvasive method for establishing mouse model for occupational disease of pulmonary fibrosis caused by silicon dust and preparation device |
| CN116712205A (en) * | 2023-06-20 | 2023-09-08 | 中南大学湘雅二医院 | Tool set for preparing acute lung injury mouse model |
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Application publication date: 20170510 |