CN110624164A - Preparation and use of rat trachea cannula equipment - Google Patents
Preparation and use of rat trachea cannula equipment Download PDFInfo
- Publication number
- CN110624164A CN110624164A CN201810656458.0A CN201810656458A CN110624164A CN 110624164 A CN110624164 A CN 110624164A CN 201810656458 A CN201810656458 A CN 201810656458A CN 110624164 A CN110624164 A CN 110624164A
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- China
- Prior art keywords
- rat
- needle
- guide wire
- guide
- trachea cannula
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- 210000003437 trachea Anatomy 0.000 title claims abstract description 41
- 238000002360 preparation method Methods 0.000 title description 3
- 238000002627 tracheal intubation Methods 0.000 claims abstract description 22
- 238000001802 infusion Methods 0.000 claims abstract description 13
- 241000252185 Cobitidae Species 0.000 claims abstract description 6
- 210000004351 coronary vessel Anatomy 0.000 claims abstract description 4
- BRUQQQPBMZOVGD-XFKAJCMBSA-N Oxycodone Chemical compound O=C([C@@H]1O2)CC[C@@]3(O)[C@H]4CC5=CC=C(OC)C2=C5[C@@]13CCN4C BRUQQQPBMZOVGD-XFKAJCMBSA-N 0.000 claims abstract description 3
- 241000700159 Rattus Species 0.000 claims description 46
- 230000029058 respiratory gaseous exchange Effects 0.000 claims description 9
- 241001465754 Metazoa Species 0.000 claims description 4
- 235000002505 Centaurea nigra Nutrition 0.000 claims description 2
- 208000004852 Lung Injury Diseases 0.000 claims description 2
- 241001073742 Mylopharodon conocephalus Species 0.000 claims description 2
- 206010069363 Traumatic lung injury Diseases 0.000 claims description 2
- 235000013305 food Nutrition 0.000 claims description 2
- 210000004072 lung Anatomy 0.000 claims description 2
- 231100000515 lung injury Toxicity 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- 239000012530 fluid Substances 0.000 claims 1
- 238000001990 intravenous administration Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 206010000891 acute myocardial infarction Diseases 0.000 abstract description 5
- 230000006378 damage Effects 0.000 description 5
- 208000027418 Wounds and injury Diseases 0.000 description 3
- 208000014674 injury Diseases 0.000 description 3
- 206010002091 Anaesthesia Diseases 0.000 description 2
- 230000037005 anaesthesia Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 206010051867 Tracheal injury Diseases 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 210000000683 abdominal cavity Anatomy 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 239000002390 adhesive tape Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000000845 cartilage Anatomy 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 238000003304 gavage Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229960001412 pentobarbital Drugs 0.000 description 1
- WEXRUCMBJFQVBZ-UHFFFAOYSA-N pentobarbital Chemical compound CCCC(C)C1(CC)C(=O)NC(=O)NC1=O WEXRUCMBJFQVBZ-UHFFFAOYSA-N 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000004202 respiratory function Effects 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 208000023504 respiratory system disease Diseases 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M16/00—Devices for influencing the respiratory system of patients by gas treatment, e.g. mouth-to-mouth respiration; Tracheal tubes
- A61M16/04—Tracheal tubes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2250/00—Specially adapted for animals
Landscapes
- Health & Medical Sciences (AREA)
- Pulmonology (AREA)
- Emergency Medicine (AREA)
- Engineering & Computer Science (AREA)
- Anesthesiology (AREA)
- Biomedical Technology (AREA)
- Heart & Thoracic Surgery (AREA)
- Hematology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Media Introduction/Drainage Providing Device (AREA)
Abstract
The invention belongs to the manufacture and use of rat trachea cannula equipment, and particularly relates to the manufacture and use of the rat trachea cannula equipment for manufacturing an acute myocardial infarction model. The needle core and the guide wires in the needle core are respectively a guide needle and a loach guide wire (Japanese Tylox super-slippery M-shaped loach guide wire) used for coronary artery intervention, the soft head end of the guide wire penetrates through the tail end of the guide needle, the head end of the guide needle is exposed by 0.4cm, and the redundant guide wire at the tail end of the guide needle is cut off. The outer layer hose is made of an infusion needle (0.5 multiplied by 20 type infusion set of Shanghai metallurgical medical apparatus Co., Ltd.) of a disposable venous infusion set, the length of the outer layer hose is 0.3cm shorter than that of the catheter needle, and the root of the outer layer hose is obliquely cut into 1 flap occupying 1/3 of the circumference of the hose. After anaesthetizing, the rat is fixed and preserved, the tongue of the rat is clamped out by a small forceps, the tongue tip is pulled by the left hand, and the trachea cannula is held by the right hand. The harder catheter needle at the head end of the tracheal intubation needle core is arranged at the root part of the tongue of a rat, and the tracheal opening of the rat is found by means of fine adjustment self-made tracheal intubation under direct vision. The trachea opening is expanded and is instantly fed into a guide wire at the head end of the trachea cannula, then the guide wire is inserted by about 1cm in a rotating mode, the catheter needle is pulled out, and if the rat breathes uniformly and smoothly, the intubation is successful.
Description
Technical Field
The invention belongs to the manufacture and use of rat trachea cannula equipment, and particularly relates to the manufacture and use of the rat trachea cannula equipment for manufacturing an acute myocardial infarction model.
Background
The trachea cannula is an essential device for respiratory function determination, respiratory tract administration and drug effect identification, respiratory system disease modeling and other experiments needing a breathing machine to support. Research proves that trachea cannula is one of the main influencing factors of death of a rat acute myocardial infarction model all the time, and the traditional trachea cannula cut through the neck has large damage and complicated operation. The damage of the trachea cannula under the condition of direct vision through the mouth is small, the operation is convenient, the trachea cannula is widely applied, but the traditional gavage needle for No. 7 mice is not easy to succeed in the trachea cannula through the mouth, and researchers research shows that the death rate of the trachea cannula through the mouth is higher than that of the trachea cannula cut through the neck, and the possibility of the study is related to the inexperience of the operation and the selection of trachea cannula equipment. The mouse stomach irrigation needle is hard, and the head end is thick and blunt, and the inventor finds the following defects in the previous experiment: the injury to the throat and trachea of a rat is large in the intubation process; moving the rat in the operation to increase the tracheal injury; the contact with the trachea is poor, and the tidal volume of the breathing machine is not easy to control. Through the verification of 150 SPF rats, the trachea intubation equipment has the one-time success rate of trachea intubation of more than 90 percent and the survival rate of an acute myocardial infarction model of the rat of more than 90 percent which is higher than that of the traditional trachea intubation method.
Disclosure of Invention
The rat trachea cannula equipment is developed by taking the clinical experience in coronary artery intervention and the trachea cannula experience in rat acute myocardial infarction model making operation as reference, and is continuously improved in the later period to form the existing perfect equipment. The equipment comprises the following parts: a guide wire ((r) in fig. 1); a transfusion needle hose (② in figure 1); the cut part of the valve ((c) in figure 1); catheter needle ((r) in fig. 1); the anti-slip tape is placed on the handle (in fig. 1). The invention has the following advantages: (1) easily-accessible raw materials and simple preparation. The needle core and the guide wires in the needle core are respectively a guide needle and a loach guide wire (Japanese Tylox super-slippery M-shaped loach guide wire) used for coronary artery intervention, the soft head end of the guide wire penetrates through the tail end of the guide needle, the head end of the guide needle is exposed by 0.4cm, and the redundant guide wire at the tail end of the guide needle is cut off. The outer layer of hose is made of an infusion needle (0.5 multiplied by 20 type infusion set of Shanghai metallurgical medical apparatus Co., Ltd.) of a disposable venous infusion set, the length of the outer layer of hose is about 0.3cm shorter than that of a catheter needle, and the root of the hose is obliquely cut into a flap occupying 1/3 of the circumference of the hose. (2) Convenient to use, trachea injury is less. When the trachea is intubated, the guide needle lightly presses the tongue root, the annular tracheal cartilage is clearly visible, the head end of the soft guide wire is inserted into the trachea under direct vision to guide the insertion of the guide steel needle, the intubatton success rate is high, and the injury of the throat and the trachea is small; when closing the chest, the flap at the starting end of the hose can prevent lung injury caused by overlarge tidal volume, the opening can be pinched according to needs when closing the chest, the lung expansion degree is controlled, accumulated liquid and accumulated gas in the chest can be better discharged, the operation can be independently completed by a single person, and the time difference for operating the breathing machine can be reduced and the pollution in the operation can be reduced. (3) The hose has good air-tube contact, reduces the leakage of air from the periphery of the intubation tube, and is easier to control the tidal volume of the respirator.
Drawings
FIG. 1 is a schematic structural diagram of the present invention.
The reference sign indicates that firstly, a guide wire is guided; a flexible tube of the infusion apparatus needle; cutting the valve; catheter needle; the anti-skid adhesive tape is held by hand.
Detailed Description
(1) Rats were deprived of food for 12 hours, water was withheld, and weighed.
(2) Abdominal cavity anesthesia: the lower abdomen and the lateral side of the abdominal midline of the rat are inserted with 0.5cm, the inclined plane of the needle head faces upwards, the angle between the needle head and the skin is 45 degrees, the depth of the insertion is about 0.5cm, and the injection is slowly injected with 0.4ml/100g of 1% sodium pentobarbital after the blood is not returned.
(3) And after 5-10 min, finishing anesthesia. The rat was fixed to a rat plate, a 10ml syringe tube was placed at the back of the neck to hold the head of the rat, and the rat plate at the head end of the rat was raised to 45 ° using a previously prepared paper box (i use small paper boxes with a length, width and height of 15cm, 7cm and 7cm, respectively) to observe the opening of the trachea.
(4) The tongue of the rat is clipped out by a small forceps, the tongue tip is pulled by the left hand, and the trachea cannula is held by the right hand.
(5) The catheter needle (the fourth part in the figure 1) with the hard head end of the tracheal intubation needle core is arranged at the root part of the tongue of the rat, and the tracheal opening of the rat is found by means of fine adjustment self-made tracheal intubation under the direct vision. The trachea opening is expanded and instantly fed into a guide wire (phi in figure 1) at the head end of a self-made trachea cannula, then the guide wire is inserted by about 1cm in a rotating mode, a catheter needle is pulled out, and if the rat breathes uniformly and smoothly, the cannula is successfully inserted.
(6) If the success of the tracheal intubation cannot be determined, a thin wire can be placed at the tail end of the tracheal intubation, and the intubation succeeds if swinging along with breathing exists; if the rat breathes hard or the thin line at the tail end of the tracheal cannula is not moved, the cannula is pulled out quickly, and the 2 nd operation or the 3 rd operation is carried out. 1 rat repeated tracheal intubation a maximum of 3 times, with 3 rd unsuccessful abandonment. After successful intubation, the 10ml syringe under the rat neck and the rat cardboard box were removed.
(7) Setting parameters of an animal breathing machine: tidal volume 9ml, breathing ratio 1.5:1, frequency 60-70 times/min. The trachea cannula is fixed at the mouth edge of the rat when the animal respirator is connected, so that the trachea cannula is prevented from being separated due to the later moving of the rat or the autonomous movement of the rat.
Claims (8)
1. The equipment comprises the following parts: guide wire, infusion apparatus needle hose, valve incision part, and catheter needle.
2. According to the claim 1, the needle core and the guide wire in the needle core are respectively a guide needle and a loach guide wire (Japanese Tylox super-slippery M-shaped loach guide wire) used for coronary artery intervention, the soft end of the guide wire penetrates through the tail end of the guide needle, the head end of the guide needle is exposed by 0.4cm, and the redundant guide wire at the tail end of the guide needle is cut off.
3. The device of claim 1, wherein the outer tube is made of infusion needle of disposable intravenous infusion set (Shanghai Yuan medical instruments Co., Ltd., 0.5X 20 type infusion set), and the length of the outer tube is about 0.3cm shorter than that of the catheter needle.
4. The infusion set hose according to claim 1, wherein a flap is obliquely cut at the root of the infusion set hose and occupies 1/3 of the circumference of the hose.
5. The device of claims 1 and 4, wherein the valve at the starting end of the tube can prevent lung injury caused by excessive tidal volume when closing the chest, and can pinch the opening according to the requirement to control the lung expansion degree and better discharge accumulated fluid and air in the chest, which can be independently completed by a single person, and can reduce the time difference for operating the respirator and reduce the pollution during operation.
6. The application steps of the device are as follows: (1) rats were deprived of food for 12 hours, water was withheld, and weighed. (2) Anaesthetizing, fixing and preparing skin. (3) The tongue of the rat is clipped out by a small forceps, the tongue tip is pulled by the left hand, and the trachea cannula is held by the right hand. (4) The catheter needle (the fourth part in the figure 1) with the hard head end of the tracheal intubation needle core is arranged at the root part of the tongue of the rat, and the tracheal opening of the rat is found by means of fine adjustment self-made tracheal intubation under the direct vision. The trachea opening is expanded and instantly fed into a guide wire (phi in figure 1) at the head end of a self-made trachea cannula, then the guide wire is inserted by about 1cm in a rotating mode, a catheter needle is pulled out, and if the rat breathes uniformly and smoothly, the cannula is successfully inserted. (5) If the success of the tracheal intubation cannot be determined, a thin wire can be placed at the tail end of the tracheal intubation, and the intubation succeeds if swinging along with breathing exists; if the rat breathes hard or the thin line at the tail end of the tracheal cannula is not moved, the cannula is pulled out quickly, and the 2 nd operation or the 3 rd operation is carried out. 1 rat repeated tracheal intubation a maximum of 3 times, with 3 rd unsuccessful abandonment. After successful intubation, the 10ml syringe under the rat neck and the rat cardboard box were removed. (6) Setting parameters of an animal breathing machine: tidal volume 9ml, breathing ratio 1.5:1, frequency 60-70 times/min.
7. The trachea cannula is fixed at the mouth edge of the rat when the animal respirator is connected, so that the trachea cannula is prevented from being separated due to the later moving of the rat or the autonomous movement of the rat.
8. According to claim 6, after anaesthetizing the rat, the rat is fixed on the rat board, the back of the neck is padded with a 10ml syringe tube to keep the head of the rat, and the rat board at the head end of the rat is padded with a prepared paper box (I use small paper boxes with the length, width and height of 15cm, 7cm and 7cm respectively) at 45 degrees so as to observe the trachea opening.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810656458.0A CN110624164A (en) | 2018-06-24 | 2018-06-24 | Preparation and use of rat trachea cannula equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810656458.0A CN110624164A (en) | 2018-06-24 | 2018-06-24 | Preparation and use of rat trachea cannula equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110624164A true CN110624164A (en) | 2019-12-31 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810656458.0A Pending CN110624164A (en) | 2018-06-24 | 2018-06-24 | Preparation and use of rat trachea cannula equipment |
Country Status (1)
| Country | Link |
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| CN (1) | CN110624164A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112126660A (en) * | 2020-09-28 | 2020-12-25 | 新疆医科大学第一附属医院 | Preparation method of animal model with high expression of gene transfected in lung tissue through intratracheal way |
| CN113425443A (en) * | 2021-06-17 | 2021-09-24 | 湖南丰晖生物科技有限公司 | Construction method of myocardial ischemia model of SD rat |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020195103A1 (en) * | 2001-03-05 | 2002-12-26 | Government Of The United States, As Represented By The Secretary Of The Army | Intubation device and method |
| CN202876007U (en) * | 2012-09-17 | 2013-04-17 | 温浩 | Rat and mouse trachea intubation device |
| CN203341849U (en) * | 2013-06-14 | 2013-12-18 | 中国人民解放军第四军医大学 | Mouse oral trachea cannula |
| CN106620998A (en) * | 2016-11-13 | 2017-05-10 | 徐兴祥 | Tracheal intubation needle for mouse and tracheal intubation method for mouse |
-
2018
- 2018-06-24 CN CN201810656458.0A patent/CN110624164A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020195103A1 (en) * | 2001-03-05 | 2002-12-26 | Government Of The United States, As Represented By The Secretary Of The Army | Intubation device and method |
| CN202876007U (en) * | 2012-09-17 | 2013-04-17 | 温浩 | Rat and mouse trachea intubation device |
| CN203341849U (en) * | 2013-06-14 | 2013-12-18 | 中国人民解放军第四军医大学 | Mouse oral trachea cannula |
| CN106620998A (en) * | 2016-11-13 | 2017-05-10 | 徐兴祥 | Tracheal intubation needle for mouse and tracheal intubation method for mouse |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112126660A (en) * | 2020-09-28 | 2020-12-25 | 新疆医科大学第一附属医院 | Preparation method of animal model with high expression of gene transfected in lung tissue through intratracheal way |
| CN113425443A (en) * | 2021-06-17 | 2021-09-24 | 湖南丰晖生物科技有限公司 | Construction method of myocardial ischemia model of SD rat |
| CN113425443B (en) * | 2021-06-17 | 2023-07-07 | 湖南丰晖生物科技有限公司 | Construction method of SD rat myocardial ischemia model |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20191231 |
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