CN106725988A - A kind of rat trachea intubation procedure and its application - Google Patents
A kind of rat trachea intubation procedure and its application Download PDFInfo
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- CN106725988A CN106725988A CN201611090859.1A CN201611090859A CN106725988A CN 106725988 A CN106725988 A CN 106725988A CN 201611090859 A CN201611090859 A CN 201611090859A CN 106725988 A CN106725988 A CN 106725988A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
- A61D7/04—Devices for anaesthetising animals by gases or vapours; Inhaling devices
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Abstract
The invention discloses a kind of rat trachea intubation procedure and its application.The inventive method is comprised the following steps:Postanesthetic rat dorsal position is placed, is fixed, the upper lower jaw of rat is pulled open, by laryngoscope exposed Rats glottis, the tracheal tube of a length of 6 ~ 7cm is inserted directly into rat trachea, i.e. glottis opening and closing place by means of the observation of laryngoscope;Tracheal tube is inserted into thin wire and injects a trifle water column;The mobile change of water column in observation sleeve pipe, if water column is motionless in sleeve pipe or fierce ejection or suction, illustrate intubation into oesophagus, it is necessary to intubation again;If the rhythmical up and down motion of water column follower respiratory rate in sleeve pipe, illustrate to insert tracheae.The inventive method is applied to the trachea cannula when rat trachea administration, rat trachea administration modeling and pulmonary function are determined, with simplicity, hurtless measure, speed fast, modeling and administration success rate is high, pulmonary function detection accurately etc. the characteristics of.
Description
Technical field
The invention belongs to endotracheal intubation field, more particularly to a kind of rat trachea intubation procedure and its application.
Background technology
Rat trachea intubation technique be usually used in rat trachea administration, rat trachea administration modeling, pulmonary function determine with
And the experimental study related to respiratory disease.It is intended into promoting the circulation of qi when rat correlation model is replicated and pulmonary function is detected
Cannula, endotracheal intubation directly affects the success or failure of the success rate and pulmonary function detection of movable model duplication, is entirely testing
During it is very crucial.
Conventional trachea cannula method is more based on blindmate method and tracheae ablation process.
Blindmate method easily inserts oesophagus, and success rate is low, it is necessary to try to insert repeatedly, easy injury rats can pharyngeal, causes secretion greatly
The mucus of amount and influence administration, especially determine PFT when, mucus plugging trachea cannula directly result in lung function index detection
Mistake, blindmate method causes wound larger, easily causes infection, and wound is difficult to recover, and increased pathology shape to animal again on the contrary
State, increased the error of whole experiment process.
Tracheotomy method needs to cut the skin of neck of rat, is successively separated exposure tracheae and tracheostomize, intubation
After tighten, this method easily causes the generation of postoperative local infection and some complication, causes secondary insult, and due to dynamic
Can not be survived for a long time after thing intubation process, it is impossible to carry out continuity Journal of Sex Research.
The content of the invention
It is an object of the invention to provide a kind of rat trachea intubation procedure, it is intended to solve existing trachea cannula method to big
Mouse has that injury, wound and experimental result have.
The present invention is achieved in that a kind of rat trachea intubation procedure, and the method is comprised the following steps:
(1)Postanesthetic rat dorsal position is placed, fixed rat four limbs, head pull open the upper lower jaw of rat;
(2)Exposed Rats glottis in rat oral cavity is stretched into by appropriately sized laryngoscope, the moment of its air-breathing is taken advantage of, by a length of 6 ~
The tracheal tube of 7cm is taken advantage of a situation insertion rat trachea;Wherein, before rat trachea is inserted, the tracheal tube is inserted into thin wire
And a trifle water column is injected, the thin wire is coiled into annular shape, the thin iron away from the tail end in sleeve pipe insertion rat trachea direction
Filament length degree is less than casing length;
(3)The mobile change of water column in observation sleeve pipe, if water column is motionless in sleeve pipe or fierce ejection or suction, illustrate intubation entrance
Oesophagus is, it is necessary to intubation again;If the rhythmical up and down motion of water column follower respiratory rate in sleeve pipe, illustrate to insert tracheae.
Preferably, in step(3)Also include step afterwards:
(4)After sleeve pipe insertion tracheae, thin wire is extracted rapidly, the syringe that will be preinstalled with parenteral solution is connected to tracheal tube
On, tracheae is disposably pushed, sleeve pipe is extracted immediately, rat is upright, and then left rotation and right rotation 1 ~ 2 minute, makes parenteral solution in rat
Intrapulmonary is evenly distributed.
Preferably, in step(1)In, the anesthesia is specially:Rat isoflurane is allowed to be anaesthetized, and by pressing from both sides tail
Without pain reaction determines whether to anaesthetize successfully.
Preferably, in step(1)In, the mode that the upper lower jaw of the rat is pulled open is:Strangled in rat by a rubber band
Upper tooth, forward downward drawing, is pulled rearwardly and upwardly with another rubber band lower tooth that fastens.
Preferably, in step(1)In, the angle for pulling rearwardly and upwardly is 60 °.
Preferably, in step(2)In, the laryngoscope is the special laryngoscope of toy;
The exposure operation of the rat glottis is specially:The hand-held laryngoscope is stretched into rat oral cavity, and laryngoscope opening is to bottom right, larynx
Mirror dorsal segment lifts exposed Rats glottis after the gently jack-up root of the tongue upwards.
Preferably, in step(2)In, the tracheal tube is to cut the single use venous transfusion after needle portion
Pipe.
Invention further provides a kind of application of rat trachea intubation procedure, the rat trachea intubation procedure include with
Lower step:
(1)Postanesthetic rat dorsal position is placed, fixed rat four limbs, head pull open the upper lower jaw of rat;
(2)Exposed Rats glottis in rat oral cavity is stretched into by appropriately sized laryngoscope, the moment of its air-breathing is taken advantage of, by a length of 6 ~
The tracheal tube of 7cm is taken advantage of a situation insertion rat trachea;Wherein, before rat trachea is inserted, the tracheal tube is inserted into thin wire
And a trifle water column is injected, the thin wire is coiled into annular shape, the thin iron away from the tail end in sleeve pipe insertion rat trachea direction
Filament length degree is less than casing length;
(3)The mobile change of water column in observation sleeve pipe, if water column is motionless in sleeve pipe or fierce ejection or suction, illustrate intubation entrance
Oesophagus is, it is necessary to intubation again;If the rhythmical up and down motion of water column follower respiratory rate in sleeve pipe, illustrate to insert tracheae.
The above method is applied when rat trachea administration, rat trachea administration modeling and pulmonary function are determined
Trachea cannula.
The present invention overcomes the deficiencies in the prior art, there is provided a kind of rat trachea intubation procedure and its application.The inventive method
Comprise the following steps:Postanesthetic rat dorsal position is placed, is fixed, the upper lower jaw of rat pulled open, by laryngoscope exposed Rats
Glottis, rat trachea, i.e. glottis opening and closing place are inserted directly into by the tracheal tube of a length of 6 ~ 7cm by means of the observation of laryngoscope;Tracheae
Sleeve pipe is inserted into thin wire(To its supporting function of sleeve pipe)And inject a trifle water column;The mobile change of water column in observation sleeve pipe,
If water column is motionless in sleeve pipe or fierce ejection or suction, illustrate that intubation enters oesophagus, it is necessary to intubation again;If in sleeve pipe water column with
The rhythmical up and down motion of animal breath frequency, illustrates to insert tracheae.
The present invention is carried out under small animal laryngoscope guiding, can avoid cutting using small animal laryngoscope guidance tracheal intubation
The method that the skin of neck of rat is successively separated and exposes tracheae, eliminate rat the postoperative local infection of incision skin and
The inducement of some complication, it is to avoid secondary insult.
Application small animal laryngoscope guides the sewing process during can reducing classical modeling during modeling, and can reduce
The consumption of anaesthetic, efficiency is can greatly improve during a large amount of animal models, it is to avoid conventional method multiple intubation is caused
Tracheal mucus secretion and wound, applying during modeling, rat can be reduced or avoided can pharyngeal inflammation, it is to avoid because stimulating
Caused mucus plugging intubation and then influence administration and lung function tests(Mucus enters or blocks intubation for lung function index
Detection is fatal), it is the improvement to previous methods, it is high with simplicity, hurtless measure, fast speed, modeling and administration success rate
Feature.
In addition, the anesthesia of animal uses the desk-top Anesthesia machine isoflurane inhalation anesthesia of experimental animal in catheterization procedure of the present invention
Method, with good anesthetic effect, anesthesia induction and recovery are very fast.Without stomodaeal nervous system excitement phenomenon during anesthesia, in body
It is interior to be seldom decomposed, discharged by respiratory tract with original shape, and traditional yellow Jackets intraperitoneal injection of anesthesia is made to the suppression for breathing
With obvious, tracheae secreting mucus are more, are unfavorable for the carrying out of intubation.
Compared to the shortcoming and defect of prior art, the invention has the advantages that:
(1)Intubation procedure of the present invention eliminates the inducement for cutting the postoperative local infection of skin and some complication of rat, it is to avoid
Secondary insult;
(2)Intubation procedure of the present invention can reduce sewing process during classical modeling, anaesthetic applying during modeling
Consumption, it is to avoid tracheal mucus secretion and wound that conventional method multiple intubation is caused, is applying during modeling, can reduce
Or avoid rat meeting pharyngeal inflammation, it is to avoid mucus plugging intubation and then influence administration and lung function tests caused by because stimulating(It is viscous
It is fatal for the detection of lung function index that liquid enters or block intubation), with simplicity, hurtless measure, speed fast, modeling and give
The characteristics of medicine success rate is high.
Specific embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
The invention discloses a kind of rat trachea intubation procedure, the method is comprised the following steps:
(1)Conventinal breeding animal, with the desk-top Anesthesia machine isoflurane inhalation anesthesia of experimental animal, after folder tail without pain is reacted,
Rat dorsal position is fixed on mouse plate, fixing limbs, fixing head, makes head slightly to layback.Assistant is strangled with a rubber band
The tooth on rat, forward downward drawing, is pulled rearwardly and upwardly with another rubber band lower tooth that fastens(Angle is advisable with 60o);
(2)Patient's left hand holds the special laryngoscope of toy, stretches into rat oral cavity, is open to bottom right, and dorsal segment is slightly upper upwards to be lifted gently
The jack-up root of the tongue, exposes glottis, the moment of animal air-breathing is taken advantage of, by homemade tracheal tube(By single use venous detaining needle
(22G×25mm/Y-G)The part of syringe needle is cut off, the length of remaining plastics sebific duct is advisable with 6~7cm, and tail end is made of thin wire
It is equal with plastics sebific duct as nook closing member, length in into annular shape insertion sebific duct, sebific duct can not be exceeded, in order to avoid stab rat throat)
Take advantage of a situation in insertion rat trachea, in advance the small water column of the section of injection 2 ~ 3 in sleeve pipe;
(3)The mobile change of water column in observation sleeve pipe, if water column is motionless in sleeve pipe or fierce ejection or suction, illustrate intubation entrance
Oesophagus is, it is necessary to intubation again;If the rhythmical up and down motion of water column follower respiratory rate in sleeve pipe, illustrate to insert tracheae.
(4)After insertion tracheae success, nook closing member is extracted rapidly, the lml syringes that will in advance take out liquid or physiological saline connect
It is connected on tracheal tube, disposably pushes tracheae.After injection, sleeve pipe is extracted immediately, animal is upright, then left rotation and right rotation about 1
Minute, medicine is evenly distributed in intrapulmonary.After animal regains consciousness naturally, conventional SPF is raised.
Rat trachea intubation procedure of the present invention is used for rat trachea administration, rat trachea administration modeling, pulmonary function survey
The fixed and experimental study related to respiratory disease.Rat correlation model replicate and pulmonary function detect when be intended into
Promoting the circulation of qi cannula, endotracheal intubation directly affects the success or failure of the success rate and pulmonary function detection of movable model duplication, whole
It is very crucial in experimentation.
This method is carried out under small animal laryngoscope guiding, can avoid cutting using small animal laryngoscope guidance tracheal intubation
The method that the skin of neck of rat is successively separated and exposes tracheae, eliminate rat the postoperative local infection of incision skin and
The inducement of some complication, it is to avoid secondary insult.The guiding of application small animal laryngoscope can reduce classical modeling during modeling
During sewing process, and the consumption of anaesthetic can be reduced, can greatly improve efficiency during a large amount of animal models.It is
Improvement to previous methods, the characteristics of with simplicity, hurtless measure, speed fast, modeling and administration success rate high.
In addition, the anesthesia of animal uses the desk-top Anesthesia machine isoflurane inhalation anesthesia of experimental animal in catheterization procedure of the present invention
Method, with good anesthetic effect, anesthesia induction and recovery are very fast.Without stomodaeal nervous system excitement phenomenon during anesthesia, in body
It is interior to be seldom decomposed, discharged by respiratory tract with original shape, and traditional yellow Jackets intraperitoneal injection of anesthesia is made to the suppression for breathing
With obvious, tracheae secreting mucus are more, are unfavorable for the carrying out of intubation.
Presently preferred embodiments of the present invention is the foregoing is only, is not intended to limit the invention, it is all in essence of the invention
Any modification, equivalent and improvement made within god and principle etc., should be included within the scope of the present invention.
Claims (8)
1. a kind of rat trachea intubation procedure, it is characterised in that the method is comprised the following steps:
(1)Postanesthetic rat dorsal position is placed, fixed rat four limbs, head pull open the upper lower jaw of rat;
(2)Exposed Rats glottis in rat oral cavity is stretched into by appropriately sized laryngoscope, the moment of its air-breathing is taken advantage of, by a length of 6 ~
The tracheal tube of 7cm is taken advantage of a situation insertion rat trachea;Wherein, before rat trachea is inserted, the tracheal tube is inserted into thin wire
And a trifle water column is injected, the thin wire is coiled into annular shape, the thin iron away from the tail end in sleeve pipe insertion rat trachea direction
Filament length degree is less than casing length;
(3)The mobile change of water column in observation sleeve pipe, if water column is motionless in sleeve pipe or fierce ejection or suction, illustrate intubation entrance
Oesophagus is, it is necessary to intubation again;If the rhythmical up and down motion of water column follower respiratory rate in sleeve pipe, illustrate to insert tracheae.
2. rat trachea intubation procedure as claimed in claim 1, it is characterised in that in step(3)Also include step afterwards:
(4)After sleeve pipe insertion tracheae, thin wire is extracted rapidly, the syringe that will be preinstalled with parenteral solution is connected to tracheal tube
On, tracheae is disposably pushed, sleeve pipe is extracted immediately, rat is upright, and then left rotation and right rotation 1 ~ 2 minute, makes parenteral solution in rat
Intrapulmonary is evenly distributed.
3. rat trachea intubation procedure as claimed in claim 2, it is characterised in that in step(1)In, the anesthesia is specific
For:Allow rat isoflurane to be anaesthetized, and determine whether to anaesthetize successfully by pressing from both sides tail without pain and reacting.
4. rat trachea intubation procedure as claimed in claim 3, it is characterised in that in step(1)In, the upper lower jaw of the rat
The mode pulled open is:The tooth on rat is strangled by rubber band, forward downward drawing is fasten lower teeth directional with another rubber band
Back upper place pulls.
5. rat trachea intubation procedure as claimed in claim 4, it is characterised in that in step(1)In, it is described to lead rearwardly and upwardly
The angle of drawing is 60 °.
6. rat trachea intubation procedure as claimed in claim 5, it is characterised in that in step(2)In, the laryngoscope is petty action
The special laryngoscope of thing;
The exposure operation of the rat glottis is specially:The hand-held laryngoscope is stretched into rat oral cavity, and laryngoscope opening is to bottom right, larynx
Mirror dorsal segment lifts exposed Rats glottis after the gently jack-up root of the tongue upwards.
7. rat trachea intubation procedure as claimed in claim 6, it is characterised in that in step(2)In, the tracheal tube is
Remove to cut the single use intravenous after needle portion.
8. the application of the rat trachea intubation procedure described in any one of claim 1 ~ 7, the application include rat trachea administration,
Rat trachea is administered the trachea cannula in the case of modeling and pulmonary function measure.
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
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CN107875169A (en) * | 2017-11-13 | 2018-04-06 | 山东省职业卫生与职业病防治研究院 | The method for building up and special Intubaction device of silicosis pulmonary fibrosis animal model |
CN109925088A (en) * | 2019-01-10 | 2019-06-25 | 青岛大学附属医院 | A kind of fixed device of dentistry experimental white mouse living body |
CN110269040A (en) * | 2019-05-28 | 2019-09-24 | 军事科学院军事医学研究院环境医学与作业医学研究所 | A kind of method for building up of particulate matter breath exposure neurotoxicity rat model |
CN110559102A (en) * | 2019-08-30 | 2019-12-13 | 南华大学 | Rat non-exposure type tracheal instillation method |
CN112189625A (en) * | 2020-10-14 | 2021-01-08 | 苏州西山中科药物研究开发有限公司 | Rat lung injury model with different severity induced by intratracheal spraying of bleomycin with different doses |
CN116712205A (en) * | 2023-06-20 | 2023-09-08 | 中南大学湘雅二医院 | Tool set for preparing acute lung injury mouse model |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107875169A (en) * | 2017-11-13 | 2018-04-06 | 山东省职业卫生与职业病防治研究院 | The method for building up and special Intubaction device of silicosis pulmonary fibrosis animal model |
CN109925088A (en) * | 2019-01-10 | 2019-06-25 | 青岛大学附属医院 | A kind of fixed device of dentistry experimental white mouse living body |
CN110269040A (en) * | 2019-05-28 | 2019-09-24 | 军事科学院军事医学研究院环境医学与作业医学研究所 | A kind of method for building up of particulate matter breath exposure neurotoxicity rat model |
CN110559102A (en) * | 2019-08-30 | 2019-12-13 | 南华大学 | Rat non-exposure type tracheal instillation method |
CN112189625A (en) * | 2020-10-14 | 2021-01-08 | 苏州西山中科药物研究开发有限公司 | Rat lung injury model with different severity induced by intratracheal spraying of bleomycin with different doses |
CN116712205A (en) * | 2023-06-20 | 2023-09-08 | 中南大学湘雅二医院 | Tool set for preparing acute lung injury mouse model |
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Application publication date: 20170531 |