CN108542549A - Tracheae epiglottis opening site-specific delivery of drugs method suitable for the noninvasive respiratory tract administration of rat - Google Patents
Tracheae epiglottis opening site-specific delivery of drugs method suitable for the noninvasive respiratory tract administration of rat Download PDFInfo
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- CN108542549A CN108542549A CN201810471892.1A CN201810471892A CN108542549A CN 108542549 A CN108542549 A CN 108542549A CN 201810471892 A CN201810471892 A CN 201810471892A CN 108542549 A CN108542549 A CN 108542549A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
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Abstract
The invention discloses the tracheae epiglottis opening site-specific delivery of drugs methods suitable for the noninvasive respiratory tract administration of rat, include the following steps：Step 1: after rat anesthesia, by rat vertical hanging on glass plate, rat oral cavity is in the experimenter visual field hereinafter, using shadowless lamp for transmitted light source；Step 2: an experimenter, which involves rat tongue after fixing rat head, is exposed to oral external, and by light-resource fousing in rat oral cavity, another one experimenter is then slowly inserted into locator by oral cavity until tracheae epiglottis, when observing white bubble caused by rat trachea breathing and floating up and down, indicate that rat trachea exposure portion is correct, Step 3: using administration device that drug is slowly dropped into pipe via locator at this time, after drug drip, locator is removed.The advantage of the invention is that：Easy to operate, safety, is suitable for high-volume zoopery, and administration accuracy is good.
Description
Technical field
The present invention relates to laboratory rat medicine-feeding technology fields, are especially suitable for the tracheae of the noninvasive respiratory tract administration of rat
Epiglottis opening site-specific delivery of drugs method.
Background technology
In contemporary life science, zoopery is indispensable.Various physiological phenomenons and pathomechanism need mostly
It to illustrate or confirm by zoopery.Safe and effective medication is to ensure that the key of Success in Experiment, is experiment knot
Fruit is accurate, true and reliable premise.With the development of science and technology, the progress of human civilization, animal welfare is also increasingly by people
Attention, ensure that experimental animal enjoys due welfare, reduce animal suffering to the greatest extent during the experiment, provided for it easypro
Suitable, painless experimental situation respects it and gives birth to the tremendous contribution that medical scientific undertakings is made for the mankind, is embodied in zoopery
One of main path of humanistic care.
Tracheal instillation is the main experimental methods of intrarterial or contamination, and most of experimental animal Intratracheal administration is at present
Invasive, and esophagus can not be avoided to be strayed into.In addition, to ensure that the accuracy of administration, experimenter use tracheotomy toward contact,
Tracheae is set to expose rat neck skin and Muscle disruption, the notch that drug is exposed via tracheae instils, or with sharp sample introduction
Device punctures tracheae to realize the purpose of tracheal instillation administration.Such method has open wound, is easy to damage experimental animal, and
With the extension of time, zoogenetic infection rate increases, it is possible to cause animal dead, it is also possible to the accuracy of experimental result is influenced,
And it is unfavorable for long-time Intratracheal administration repeatedly；In addition, tracheotomy method is complicated, larger manpower and time are needed, it can not
Suitable for high-volume zoopery.
Invention content
The technical problem to be solved by the invention for the present situation of prior art is to provide easy to operate, safety, are suitable for
High-volume zoopery, the good tracheae epiglottis opening site-specific delivery of drugs side suitable for the noninvasive respiratory tract administration of rat of administration accuracy
Method.
Technical solution is used by the present invention solves above-mentioned technical problem：
Suitable for the tracheae epiglottis opening site-specific delivery of drugs method of the noninvasive respiratory tract administration of rat, include the following steps：
Step 1: after rat anesthesia, by rat vertical hanging on glass plate, rat oral cavity in the experimenter visual field hereinafter, using
Shadowless lamp is transmitted light source；
Step 2: an experimenter, which involves rat tongue after fixing rat head, is exposed to oral external, and by light source
Rat oral cavity is focused on, another one experimenter is then slowly inserted into locator by oral cavity until tracheae epiglottis, big when observing
White bubble caused by mouse tracheal respiration and when floating up and down, indicates that rat trachea exposure portion is correct,
Step 3: use administration device that drug is slowly dropped into pipe via locator at this time, it, will after drug drip
Locator removes.
The technical measures of optimization further include：
In above-mentioned step three, after drug drip, rat lung sound is listened with stethoscope, when hearing the moist hello of rat lung
When sound, indicate that drug can smoothly enter into tracheae.
Above-mentioned administration device is microsyringe or blunt nosed sample introduction needle.
Above-mentioned locator is made of retainer body and handle, and retainer body is in up big and down small conical hollow knot
Structure, handle are laterally fixedly connected on the upper end of the retainer body, the lower end diameter of retainer body be 0.2cm extremely
0.4cm。
The lower end of the longitudinal cross-section of above-mentioned retainer body is spherical structure.
The upper end diameter of above-mentioned retainer body is 1cm to 1.2cm.
The height of above-mentioned retainer body is 5cm to 5.5cm.
The length of above-mentioned handle is 2cm to 3cm.
The width of above-mentioned handle is 0.8cm to 1.2cm.
Above-mentioned locator is made of food-grade high molecular polymer.
The present invention is suitable for the tracheae epiglottis opening site-specific delivery of drugs method of the noninvasive respiratory tract administration of rat, uses noninvasive behaviour
Make, will not injury rats tracheae, air pipe inlet is high-visible in administration process, and drug can be avoided to be strayed into esophagus, this gives prescription
Method drip-injection method is simple, and dosage is accurate, can freely control dosage；Survival rate is high after animal tracheal instillation.To sum up
Described, not only drip-injection method is accurate, conveniently, safely quick and not damaged to rat trachea for this tracheal instillation method, can be extensive
Applied to rat trachea dropleting medicine-feeding.
Description of the drawings
Fig. 1 is the structural schematic diagram of locator in the present invention；
Fig. 2 is the cutaway view Amplified image of locator longitudinal cross-section lower end in the present invention.
Specific implementation mode
Below in conjunction with attached drawing embodiment, present invention is further described in detail.
It is as shown in Figure 1 to Figure 2 the structural schematic diagram of the present invention,
Reference numeral therein is：Height H, length L, retainer body 1, spherical structure 11, handle 2.
Suitable for the tracheae epiglottis opening site-specific delivery of drugs method of the noninvasive respiratory tract administration of rat, include the following steps：
Step 1: after rat anesthesia, by rat vertical hanging on glass plate, rat oral cavity in the experimenter visual field hereinafter, using
Shadowless lamp is transmitted light source；
Step 2: an experimenter, which involves rat tongue after fixing rat head, is exposed to oral external, and by light source
Rat oral cavity is focused on, another one experimenter is then slowly inserted into locator by oral cavity until tracheae epiglottis, big when observing
White bubble caused by mouse tracheal respiration and when floating up and down, indicates that rat trachea exposure portion is correct,
Step 3: using microsyringe or blunt nosed sample introduction needle to be slowly dropped into drug via locator as administration device at this time
Pipe after drug drip, listens rat lung sound with stethoscope, when hearing rat lung moist rales, indicates
Drug can smoothly enter into tracheae, remove locator after the completion of drug drip.
In embodiment, locator is made of retainer body 1 and handle 2, and retainer body 1 is in up big and down small hollow cone
Shape structure, handle 2 are laterally fixedly connected on the upper end of the retainer body 1, and the lower end diameter of retainer body 1 is 0.2cm
To 0.4cm.
In embodiment, the lower end of the longitudinal cross-section of retainer body 1 is spherical structure 11.The setting of spherical structure 11, energy
When enough locator being avoided to be inserted into, the oral cavity of rat is caused to scratch.
In embodiment, the upper end diameter of retainer body 1 is 1cm to 1.2cm.
In embodiment, the height H of retainer body 1 is 5cm to 5.5cm.
In embodiment, the length L of handle 2 is 2cm to 3cm.
In embodiment, the width of handle 2 is 0.8cm to 1.2cm.
In embodiment, locator is made of food-grade high molecular polymer.Such as food-grade PP, food-grade PE etc..
Using the retainer body 1 of up big and down small conical hollow structure, facilitate the irradiation of transmitted light source, rat can be made
Tracheae fully exposes, and drug drip process is enable to be completed rapidly and accurately；And the setting of handle 2, not only facilitate locator
It holds, is also convenient for being inserted into rat oral procedures to be fixed by locator adjusting with direction.Coordinate rat physiological characteristic, be
Rat trachea is set fully to expose, the lower end diameter of retainer body 1 is 0.2cm to 0.4cm；In addition coordinate rat
The height of oral cavity structure, retainer body 1 is 5cm to 5.5cm.
The tracheal instillation administration method of the present invention, using noninvasive operation, will not injury rats tracheae, operating method is accurate,
Conveniently, safely；During instillation, tracheae is high-visible, and drug can be avoided to be strayed into esophagus, and drip-injection method is simple, dosage
Accurately, dosage can be freely controlled, micro- dosage and larger dose are all applicable.
Highly preferred embodiment of the present invention has illustrated, and the various change or remodeling made by those of ordinary skill in the art are not
It can depart from the scope of the present invention.
Claims (10)
- The site-specific delivery of drugs method 1. the tracheae epiglottis suitable for the noninvasive respiratory tract administration of rat is open, it is characterized in that：Including following step Suddenly：Step 1: after rat anesthesia, by rat vertical hanging on glass plate, rat oral cavity in the experimenter visual field hereinafter, using Shadowless lamp is transmitted light source；Step 2: an experimenter, which involves rat tongue after fixing rat head, is exposed to oral external, and by light source Rat oral cavity is focused on, another one experimenter is then slowly inserted into locator by oral cavity until tracheae epiglottis, big when observing White bubble caused by mouse tracheal respiration and when floating up and down, indicates that rat trachea exposure portion is correct,Step 3: use administration device that drug is slowly dropped into pipe via locator at this time, it, will after drug drip Locator removes.
- The site-specific delivery of drugs method 2. the tracheae epiglottis according to claim 1 suitable for the noninvasive respiratory tract administration of rat is open, It is characterized in that：In the step three, after drug drip, rat lung sound is listened with stethoscope, when hearing rat lung When moist rales, indicate that drug can smoothly enter into tracheae.
- The site-specific delivery of drugs method 3. the tracheae epiglottis according to claim 2 suitable for the noninvasive respiratory tract administration of rat is open, It is characterized in that：The administration device is microsyringe or blunt nosed sample introduction needle.
- The site-specific delivery of drugs method 4. the tracheae epiglottis according to claim 3 suitable for the noninvasive respiratory tract administration of rat is open, It is characterized in that：The locator is made of retainer body (1) and handle (2), under the retainer body (1) is presented greatly Small conical hollow structure, the handle (2) are laterally fixedly connected on the upper end of the retainer body (1), and described determines The lower end diameter of position device main body (1) is 0.2cm to 0.4cm.
- The site-specific delivery of drugs method 5. the tracheae epiglottis according to claim 4 suitable for the noninvasive respiratory tract administration of rat is open, It is characterized in that：The lower end of the longitudinal cross-section of the retainer body (1) is spherical structure (11).
- The site-specific delivery of drugs method 6. the tracheae epiglottis according to claim 5 suitable for the noninvasive respiratory tract administration of rat is open, It is characterized in that：The upper end diameter of the retainer body (1) is 1cm to 1.2cm.
- The site-specific delivery of drugs method 7. the tracheae epiglottis according to claim 6 suitable for the noninvasive respiratory tract administration of rat is open, It is characterized in that：The height (H) of the retainer body (1) is 5cm to 5.5cm.
- The site-specific delivery of drugs method 8. the tracheae epiglottis according to claim 7 suitable for the noninvasive respiratory tract administration of rat is open, It is characterized in that：The length (L) of the handle (2) is 2cm to 3cm.
- The site-specific delivery of drugs method 9. the tracheae epiglottis according to claim 8 suitable for the noninvasive respiratory tract administration of rat is open, It is characterized in that：The width of the handle (2) is 0.8cm to 1.2cm.
- The site-specific delivery of drugs method 10. the tracheae epiglottis according to claim 9 suitable for the noninvasive respiratory tract administration of rat is open, It is characterized in that：The locator is made of food-grade high molecular polymer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810471892.1A CN108542549A (en) | 2018-05-17 | 2018-05-17 | Tracheae epiglottis opening site-specific delivery of drugs method suitable for the noninvasive respiratory tract administration of rat |
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|---|---|---|---|
| CN201810471892.1A CN108542549A (en) | 2018-05-17 | 2018-05-17 | Tracheae epiglottis opening site-specific delivery of drugs method suitable for the noninvasive respiratory tract administration of rat |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110559102A (en) * | 2019-08-30 | 2019-12-13 | 南华大学 | Rat non-exposure type tracheal instillation method |
| CN111281598A (en) * | 2020-03-05 | 2020-06-16 | 纳菲(深圳)制药科技有限公司 | Anesthesia control system and method |
| CN112842605A (en) * | 2021-01-12 | 2021-05-28 | 中国人民解放军海军特色医学中心 | Rat double-sleeve trachea cannula device |
-
2018
- 2018-05-17 CN CN201810471892.1A patent/CN108542549A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110559102A (en) * | 2019-08-30 | 2019-12-13 | 南华大学 | Rat non-exposure type tracheal instillation method |
| CN111281598A (en) * | 2020-03-05 | 2020-06-16 | 纳菲(深圳)制药科技有限公司 | Anesthesia control system and method |
| CN111281598B (en) * | 2020-03-05 | 2023-12-08 | 纳菲(深圳)制药科技有限公司 | Anesthesia control system and method |
| CN112842605A (en) * | 2021-01-12 | 2021-05-28 | 中国人民解放军海军特色医学中心 | Rat double-sleeve trachea cannula device |
| CN112842605B (en) * | 2021-01-12 | 2023-11-10 | 中国人民解放军海军特色医学中心 | Double-sleeve tracheal intubation device for rats |
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Application publication date: 20180918 |
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