CN210019779U - Accurate quantitative non-exposure type trachea instillator - Google Patents
Accurate quantitative non-exposure type trachea instillator Download PDFInfo
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- CN210019779U CN210019779U CN201822008690.1U CN201822008690U CN210019779U CN 210019779 U CN210019779 U CN 210019779U CN 201822008690 U CN201822008690 U CN 201822008690U CN 210019779 U CN210019779 U CN 210019779U
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Abstract
The utility model discloses a non-formula trachea instillator that exposes of accurate ration, keep somewhere the needle including the modified vein, the vein is kept somewhere the needle and has only kept syringe needle portion, and there is the hose parcel steel needle outer end, is connected with the syringe through negative and positive joint, and the drip liquid is by the accurate ration of micropipettor in the centrifuging tube, absorbs by the syringe again, takes out one section air earlier before absorbing the drip liquid with the syringe, can guarantee to move the whole instillations entering trachea of the liquid of getting, still can squeeze into the air after instillating and make the drip liquid homodisperse. The utility model has the characteristics of convenient operation, the ration is accurate, reduces the instillation in-process animal damage, provides convenient for the research of medicine lung tissue damage, can the wide application in the trachea instillation animal model's of dosing establishment.
Description
Technical Field
The utility model belongs to animal experiment auxiliary device field relates to an accurate quantitative non-exposure formula trachea instillator.
Background
Tracheal instillation is a major method of transtracheal administration or contamination of animals and is a common exposure route in the fields of medical and environmental toxicology studies. Tracheal instillation can be divided into an exposed type and a non-exposed type according to whether the trachea is exposed or not in the instillation process. The exposed tracheal instillation method usually comprises the steps of cutting open skin and muscle of the neck of an experimental animal to expose the position of a trachea, inserting a pipettor into the trachea, ensuring the accuracy of the experiment, wherein the flathead or pointed micropipettor is too thin and too hard relative to the oral cavity, throat and trachea tissues of the tested animal, so that the respiratory tract of the experimental animal is easily damaged, and the damage caused by the cut-open neck easily causes certain influence on the result of the exposure experiment. The non-exposure type tracheal instillation method is characterized in that a blunt vein indwelling needle or a puncture needle is adopted for tracheal intubation, and the method has the advantage of non-invasiveness.
An improved inhalation type tracheal instillation method is provided, namely, after an animal is anesthetized, the mouse or rat upper incisors are hung by silk threads, the animal is naturally hung on a self-made plastic frame, the larynx is fully exposed, the operation lamp irradiates the larynx, a pipettor is used for sucking the instillation liquid, the instillation liquid is injected into an injection hole, the nasal cavity of the mouse or rat is slightly pinched for about 5s, the instillation liquid is actively inhaled into the lung through respiration, and if moist rale is heard, the instillation success is indicated. The method is simple and convenient to operate, but the success rate of drip is low, and if the esophagus is inhaled, the exposure fails; and air can not be injected into the trachea through the micropipettor after the instillation of the micropipettor is finished.
SUMMERY OF THE UTILITY MODEL
The method is characterized in that the non-invasiveness and the accuracy are difficult to be considered at the same time in the current tracheal instillation method. The utility model discloses developed a non-formula trachea instillator that exposes of accurate ration, adopted micropipettor and modified vein to keep somewhere the needle and combine, realized not having the wound and the accuracy nature of instillation experiment, be the trachea instillation means of present ideal.
The utility model aims at overcoming the defects that the prior non-exposure type trachea instillation technology can not realize the accurate quantification of the instillation liquid and can not avoid the mechanical damage of animals, and providing the trachea instillation device with accurate quantification.
In order to achieve the above object, the utility model adopts the following technical scheme:
a non-exposed tracheal instillation device comprising a straight tubular injector and an improved venous indwelling needle; the venous indwelling needle comprises a steel needle, an extension tube and a front three-way hose; the steel needle is communicated with a branch I of the front three-way hose; the extension pipe is communicated with a branch II of the front three-way hose; the improved venous indwelling needle is a partial structure with an extension tube cut off, and a branch II of a three-way hose is left with a part of about 1 cm; the needle head part of the injector is communicated with a branch II of the three-way hose.
Preferably, the needle head of the syringe is detachably communicated with the branch II of the three-way hose through a two-way joint (the two-way joint is preferably a male-female joint)
Preferably, the steel needle is provided with an outer sleeve, the inner diameter of the sleeve is 0.5mm, and the length of the sleeve is 4-5 cm.
Preferably the end of the outer sleeve has a blunt outer surface.
Preferably, the outer sleeve is made of rubber.
The micro pipettor is used for realizing accurate quantification, and the venous indwelling needle is connected with the connector and the injector to realize tracheal instillation.
The existing venous indwelling needle generally comprises a steel needle, a front tee joint, an extension tube and a rear tee joint; the steel needle is communicated with a branch I of the three-way pipe; one end of the extension pipe is communicated with a branch II of the three-way hose, and the other end of the extension pipe is communicated with the rear three-way. The conventional vein indwelling needle is connected with an injector through an extension tube, and a large amount of drip liquid is remained at a hose and the like by directly using the vein indwelling needle to realize trachea drip, so that the drip amount is inaccurate. The improved venous indwelling needle is a local structure left after an extension tube and a rear tee joint are cut off, the improved venous indwelling needle is cut from a hose, only the needle head part is reserved, a rubber sleeve is wrapped outside the needle head, the inner diameter of the rubber sleeve is 0.5mm, the length of the rubber sleeve is 4-5cm, and the length is 1-2 mm when the needle head of the venous indwelling needle is completely inserted into the sleeve. The tail end of the sleeve is passivated, so that the improved venous indwelling needle has the hardness of a steel needle to facilitate intubation, and the outer side of the steel needle is wrapped by a rubber sleeve, so that the mechanical damage of the tracheal intubation to animals can be reduced to the maximum extent.
The branch II of the three-way hose is reserved with a part of about 1cm, the hose can be connected with a plastic male-female connector, the other end of the connector can be connected with an injector, and the injector and the male-female connector can be detachably connected, so that different amounts of drip liquid can be conveniently moved.
This trachea instillation device utilizes micropipettor to realize accurate ration, and the instillation liquid is earlier by the micropipettor ration in the centrifuging tube, and the rethread syringe realizes instilling, and the micropipettor can select for use different ranges, also can move many times and get convenient and fast.
The drip liquid is precisely quantified by a micropipettor, a section of air is pumped out before the syringe sucks the drip liquid, the air is pushed into the air pipe after instillation, the liquid which is moved can be ensured to be instilled into the air pipe completely, the uniform dispersion of the drip liquid is ensured, and the air can be pumped into the syringe after instillation to ensure the uniform dispersion of the drip liquid.
The needle head is wrapped by a rubber sleeve, the inner diameter of the rubber sleeve is 0.5mm, the length of the rubber sleeve is 4-5cm, the length is that the tail end of the sleeve is exposed by 1-2 mm when the needle head of the venous indwelling needle is completely inserted, and the tail end of the sleeve is passivated.
Compared with the prior art, the technical scheme in the utility model, have advantages such as simple quick, accurate ration, good reproducibility. The part of the dripping device contacting with the experimental animal is wrapped by the hose, so that the mechanical damage to the animal can be reduced; the range of the instillation amount of the test object is wide, and the method can be used for trachea instillation of small and medium-sized animals such as mice, rabbits, dogs and the like. The micropipettor is used for quantifying accurately and easily controlled, and the accuracy and the scientificity of the instillation experiment are improved.
Drawings
Fig. 1 is a structural diagram of the device of the present invention.
Detailed Description
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
The invention will be further described with reference to the accompanying drawings and the detailed description
Accurate ration trachea instillator (fig. 1), including modified vein detain needle 1, elements such as negative and positive joint 2 and syringe 3 are constituteed, wherein vein detains the needle syringe needle parcel by rubber sleeve, and the sleeve pipe is terminal through passivation treatment, and this trachea instillator utilizes the micropipettor to realize accurate ration, and the drip liquid is by the accurate ration of micropipettor in 2mL centrifuging tube.
The improved venous indwelling needle is cut from the hose, only the needle part and the hose of 1cm are reserved, the rubber sleeve is wrapped outside the needle head, the inner diameter of the rubber sleeve is 0.5mm, the needle head can be smoothly inserted, and the instillation device is inserted into the trachea to leave enough space to enable a rat to normally breathe, and the sleeve and the needle are too thick and easily block the trachea to cause animal asphyxiation. The sleeve pipe length is 5cm, and sleeve pipe end can expose 2mm when guaranteeing that the vein is kept somewhere the needle syringe needle and is inserted completely, and the sleeve pipe end is through passivation treatment, keeps mellow and avoids fish tail trachea inner wall. The venous indwelling needle is connected with the injector through a plastic male-female connector, one section of the male-female connector is connected with a reserved 1cm hose of the venous indwelling needle, the other end of the male-female connector is connected with the injector, and the injector, the male-female connector and the indwelling needle can be conveniently detached.
Use the SD rat as the animal under test, two people cooperate earlier to rat anesthesia weighing, are fixed in on the operation panel fully to expose the throat to illumination of handle art lamp is to the throat, can see trachea opening position along with the rat breathing this moment, inserts gently to trachea depths with the one end of rubber sleeve pipe blunt circle, and the syringe is connected to the other end. Before instillation, the instillation liquid is accurately quantified in a 2mL centrifugal tube by a micropipettor in advance, the syringe is used for extracting one section of air from the centrifugal tube before the instillation liquid is moved, the air is pushed into an air pipe after instillation, and the air enters the air pipe to ensure the uniform dispersion of the instillation liquid after the instillation liquid completely enters the air pipe. The entire instillation process can be completed in 30 seconds. When carrying out the trachea instillation to the toy, adopt ether anesthesia and intravenous route anesthesia homoenergetic to satisfy the requirement, including the anesthesia, fixed and instillation's whole operation process can be accomplished in 1 ~ 2 minutes.
Adopt the utility model discloses the device carries out the trachea to 70 SD rats and drips the toxicant exposure, and the anesthesia mode is anesthesia of 10% chloral hydrate 3mL/kg weight intraperitoneal injection, and the drip liquid is normal saline or the PM of different concentrations2.5The instillation amount is carried out according to 1mL/kg body weight, moist can be heard in the instillation process, the instillation success is shown, 70 rats do not die after instillation, and the device can achieve the effects of rapidness, no wound, accuracy, high survival rate and the like on tracheal instillation exposure.
Claims (5)
1. A non-exposure type tracheal instillation device is characterized in that,
the device comprises a straight tube type injector and an improved vein indwelling needle;
the venous indwelling needle comprises a steel needle, an extension tube and a front three-way hose; the steel needle is communicated with a branch I of the front three-way hose; the extension pipe is communicated with a branch II of the front three-way hose;
the improved venous indwelling needle is a partial structure with an extension tube cut off, and a branch II of a three-way hose is left with a part of about 1 cm;
the needle head part of the injector is communicated with a branch II of the three-way hose.
2. The non-exposed tracheal instillation device of claim 1, wherein the needle of the syringe is in detachable communication with branch II of the three-way hose via a two-way.
3. The non-exposed tracheal instillation device of claim 1, wherein the tracheal instillation device comprises a lumen,
the steel needle is provided with an outer sleeve, the inner diameter of the sleeve is 0.5mm, and the length of the sleeve is 4-5 cm.
4. The non-exposed tracheal instillation device of claim 3, wherein the tracheal instillation device comprises a lumen,
the end of the outer sleeve has a passivated outer surface.
5. The non-exposed tracheal instillation device of claim 3, wherein the tracheal instillation device comprises a lumen,
the outer sleeve is made of rubber.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201822008690.1U CN210019779U (en) | 2018-11-30 | 2018-11-30 | Accurate quantitative non-exposure type trachea instillator |
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| CN201822008690.1U CN210019779U (en) | 2018-11-30 | 2018-11-30 | Accurate quantitative non-exposure type trachea instillator |
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| CN210019779U true CN210019779U (en) | 2020-02-07 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113876457A (en) * | 2021-09-23 | 2022-01-04 | 安徽理工大学 | Economical, quantitative and noninvasive method for establishing mouse model for occupational disease of pulmonary fibrosis caused by silicon dust and preparation device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113876457A (en) * | 2021-09-23 | 2022-01-04 | 安徽理工大学 | Economical, quantitative and noninvasive method for establishing mouse model for occupational disease of pulmonary fibrosis caused by silicon dust and preparation device |
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