CN105434081A - Accurate, quick and noninvasive method for instilling medicine into rat - Google Patents
Accurate, quick and noninvasive method for instilling medicine into rat Download PDFInfo
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- CN105434081A CN105434081A CN201510847643.4A CN201510847643A CN105434081A CN 105434081 A CN105434081 A CN 105434081A CN 201510847643 A CN201510847643 A CN 201510847643A CN 105434081 A CN105434081 A CN 105434081A
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Abstract
The invention provides an accurate, quick and noninvasive method for instilling a medicine into a rat. According to the method, the rat is narcotized by aether and hung on an iron stand, a tongue of the rat is clamped and pulled out of a mouth by tweezers coating with gauze, a glottis is found under the exposure of a laryngoscope, a nose of the rate is squeezed to enable the rat to breathe by the mouth, a pipette with the sucked instillation medicine is inserted into the glottis quickly with the opening and closing of the glottis, and the medicine is instilled into a trachea. The pipette is taken out, a user squeeze the nose continuously for 3-8 seconds to enable the instillation medicine to be inhaled automatically through the breath of the rat, and the instillation is successful when the user hears moist rale. The accurate, quick and noninvasive method for instilling the rat has the advantages of being noninvasive, free of pain, economical, quick and accurate and has remarkable advantages compared with other instillation methods.
Description
Technical field
The present invention relates to clinical medicine and zoopery field, specifically, relate to a kind of precisely quick non-invasive rat drip-injection method.
Background technology
It is clinical medicine that respiratory tract exposes, toxicology, one of of paramount importance route of exposure in the area researches such as environmental medicine, along with the develop rapidly of each disciplinary study in recent years, through respiratory tract administration, contamination, the zooperal demand of monitoring of respiration is in rising trend, rat is international standardized laboratory animal, at plurality of medical, extensive use in biological test research, assisted mechanical ventilator is needed at rat open chest surgery, in the zoopery such as respiratory function detection or intrarterial, rat trachea intubate is the prerequisite of experiment success or failure smoothly, at present, conventional intubation procedure has: cervical incision intubation (Qiu Yongming, Tian Xin, the easy tracheal intubation method [J] of rat. Shanghai Second Emdical University's journal, 2001, 21 (6): 567), cervical incision steel wire guide cannula method (Tao Yunming, Mo Xuming, Qi Jirong etc., a kind of new rat trachea intubation procedure [J], China's experimental surgery magazine, 2004, 21 (4)): 504, WekslerB, NgB.LenertJ.Asimplifiedmethodforendotrochealintubationin therat [J] .JoumalofAppliedPhysiology.1994.76 (4): 1823), blindmate method, Endoscope-assisted intubation (ClaryEM.OHalloranEK, delaFuente.SG.etal.Videonendoscopicendortachealintubatio noftherat [J] .LabAnim, 2004.38 (2): 158), direct vision orotracheal intubation method and through cervical region transillumination endotracheal intubation (Wang Xiangying, Wu Shuyan, Li Suan, Deng, rat direct-view tracheal intubation method [J]. Shanghai Laboratory Animal Science, 2004, 24 (2): 107) etc.
Tracheal instillation method can be divided into exposure formula and non-exposed formula two kinds, namely rat trachea is come out by operation by exposure formula, and otch carries out intubate again; Non-exposed formula mainly do not need expose trachea, mainly by oral cavity by the aid such as diaphotoscope, laryngoscope, utilize cold light source to find trachea, then with conduit insert trachea instil.Exposure formula tracheal instillation in the past needs to cut the skin of rat neck and muscle to expose trachea, again micro-sample injector is inserted trachea, the accuracy that historical facts or anecdotes is tested is guaranteed, but micro-sample injector of tip easily causes rats breathing road to damage, though intubate is fast and convenient after tracheotomy, larger to animal body injury.Tissue injury and postoperative infection all can affect the recovery of postoperative Organism of Rats, add the impact of non-empirical factor on experimental result.Non-exposed formula tracheal instillation adopts the venous detaining needle of blunt or puncture needle to carry out intubate, although have Non-Invasive advantage, but because these cannula needles often mate with ordinary syringe, and the degree of accuracy of ordinary syringe is not as micro-sample injector, and non-exposed formula tracheal instillation method is difficult to take into account non-invasive and accuracy.Research display, nasal cavity instils and can reach the object of respiratory tract administration or contamination, but still not relevant research is evaluated it.
In recent years, respirator assisted ventilation techniques starts to be applied to rodent, for the Research of Animal Model for Study of the specialties such as cardiothoracic surgery, Anesthesia Department, pharmacy provides Breathing Suppotion means.Fast and effectively tracheal intubation is the prerequisite of application assisted ventilation techniques, exposure formula tracheal instillation in the past needs to cut the skin of rat neck and muscle to expose trachea, again micro-sample injector is inserted trachea, the accuracy that historical facts or anecdotes is tested is guaranteed, but micro-sample injector of tip easily causes rats breathing road to damage, though intubate is fast and convenient after tracheotomy, larger to animal body injury.Tissue injury and postoperative infection all can affect the recovery of postoperative Organism of Rats, add the impact of non-empirical factor on experimental result.Non-exposed formula tracheal instillation adopts the venous detaining needle of blunt or puncture needle to carry out intubate, although have Non-Invasive advantage, because these cannula needles often mate with ordinary syringe, and the degree of accuracy of ordinary syringe is not as micro-sample injector.Therefore, there is the intubation procedure of wound can cause damage to trachea and bottleneck throat surrounding tissue, thus affect repeating of intubate, and then affect the result of whole experiment; And adopt lower guide wire guide cannula (VLGIT) method of direct-view, and although be non-invasive tracheal instillation, operation of instiling, every animal time used is long; Dropped amount is inaccuracy still, and intubation can increase the misery of mice equally with uncomfortable.
Summary of the invention
The present invention is in view of the defect existed in existing tracheal instillation method and deficiency, for ensure rat instil can reach fast, precisely, noinvasive and the effect such as survival rate is high, start with from the aspect such as equipment, anesthesia, operating procedure of tracheal instillation and carry out Innovation, a kind of accurate quick noninvasive rat drip-injection method is provided.
In order to realize the object of the invention, one of the present invention is quick non-invasive rat drip-injection method precisely, described method is after rat etherization, is suspended on iron stand, lives outside pull-out oral cavity with the tweezers of looping gauze by Rat Tongue head-clamp, glottis is found under laryngoscope irradiates, then pinch rat nose, rat mouth is breathed, along with glottis folding, to inhale the liquid-transfering gun quick insertion glottis of instillation medicine, by drug drip to trachea; Exit liquid-transfering gun, continue to pinch nose 3-8s, make it pass through to breathe automatically by instillation medicine suction pulmonary, moist rales to be heard, shows to instil successfully.
After instillation terminates, rat is continued to be suspended on 5-10s on iron stand, keep health naturally to hang, slowly rotate rat, make medicaments uniformity be distributed to rat lung.
Aforesaid method, is placed in anesthesia tank (such as glass jar or transparent plastic can) with cover and anaesthetizes by rat, be placed with the Cotton Gossypii or gauze of dipping in full ether in anesthesia tank.
Aforesaid method, after rat etherization, hitches rat front tooth with cord, is suspended on iron stand.
Preferably, each instillation dosage control is within 300 μ l, and dropped amount, at 300 more than μ l, can be divided into and slowly instiling for twice.
Object of the present invention can also be further achieved by the following technical measures.
1, instillation early-stage preparations
Equipment needed thereby in instillation and instrument are ready in advance, comprise: iron stand, liquid-transfering gun, rifle head, children's's laryngoscope, thin wire or cord, anesthesia tank, absolute ether, Cotton Gossypii or gauze, timer.
2, anaesthetize the making of tank: for the effect of rat anesthesia can be observed intuitively, select a clear glass or plastic tank, select anesthesia tank size according to rat build.Cotton Gossypii or gauze are put into tank, and most major general anaesthetizes pot bottom and covers, and then, absolute ether is injected tank, for avoiding rat body to be infected with too many ether, it is suitable that injection rate does not ooze ether with Cotton Gossypii or gauze.General rat can reach the effect of holonarcosis in 5-10s, after operation repeatedly, optionally can add absolute ether, to ensure to anaesthetize efficiency.
3, the making of simplified operating table: get iron stand one will iron clamp or iron ring be a cord that can bear rat weight, raises the height of iron clamp or iron ring, opens to the rat tensible that front tooth can be made to hang on rope.
4, rat anesthesia: capture the rat for instiling, fast sealing is in anesthesia tank, cover tightly anesthesia tank, rat is anaesthetized in 5-10s, and anesthesia standard is motionless for falling down, and breathes slower, seemingly to sleep soundly shape, get from tank except rat fast, upper for rat front tooth is suspended on the cord of iron stand, makes it naturally hang.
5, trachea is located: after operating according to step 4, Rat Tongue head-clamp is lived outside pull-out oral cavity by one people's right hand tweezers of bag one deck mull, forward tongue is pulled out gently with left hand thumb, forefinger, the right hand puts down tweezers, irradiate along the root of the tongue from oral cavity by children's's laryngoscope, clearly can see that the glottis of rat is opened along with breathing and closes as fish mouth.
6, drug drip: after operating by step 5, another people's left hand pinches rat nose, and rat mouth is breathed, and one one, visible glottis closes, and the right hand holds the liquid-transfering gun quick insertion glottis having inhaled instillation medicine, by drug drip to trachea.Exit liquid-transfering gun, left hand continues to pinch nose 3-8s, makes it pass through to breathe initiatively by instillation medicine suction pulmonary, if hear moist rales, then illustrates and instil successfully.
7, medicaments uniformity distribution: rat continues to be suspended on 5-10s on cord, slowly rotates rat, make medicaments uniformity be distributed to rat lung after instiling and terminating.
Rat drip-injection method provided by the invention has noinvasive, no pain, economy, the feature such as quick, accurate, has good effect and advantage compared to other instillation modes:
(1) through facts have proved in a large number, this method success rate is high, has carried out 5 modelings in test to 25 SD rats, and instil accumulative 120 times to rat, success 116 times, success rate is up to 96.67%.
(2) anesthesia of the present invention belongs to non-invasive, the anesthesia of no sequel formula, anesthesia method is in conjunction with atropine substantially with pentobarbital sodium or chloral hydrate before this, need double injection, increase the misery of laboratory animal, simultaneously, also injection will be made to accidentally injure rat probability improve, further, the anaesthetic effect of this medicine is not easy to control, anaesthetized shallow rat easily in operation clear-headed struggle cause accidental injury, too dark rats breathing speed is too low, easily suffocates or choke to death after instillation medicine.If be not combined atropine in anesthesia, rat is after pentobarbital sodium or chloral hydrate, and tracheal strips produces more mucus, also can cause the danger suffocated.The present invention adopts ether to combine self-control anesthesia tank can avoid above-mentioned drawback, reaches the desirable anaesthetic effect of noinvasive, no sequel.
(3) instillation of the present invention belongs to the instillation of hurtless measure safety type, instillation mode in the past, the endotracheal intubation of exposure formula and pars cervicalis tracheae incision cannula method (TTIT) are directly cut skin, are exposed trachea, certain mechanical trauma is caused to rat in operation, postoperative also exist infection risk, and guide wire guide cannula method (VLGIT) under the direct-view of non-exposed formula, need hard steel wire to import intubate equally, there is the possibility of the rat trachea that stabs.
(4) drip-injection method of the present invention belong to fast, precisely formula instil, exposure formula in the past and non-exposed formula, because of trachea exposure, tracheorrhaphy, the step that the insertion of conduit etc. are loaded down with trivial details, consumes a large amount of time, generally every rat time of instiling needs 40s-300s, the present invention only needs 15s-30s the time of instiling, and previous methods major part adopts syringe or injector, assisting of additional conduit, add the consumption of medication amount, the present invention directly adopt liquid-transfering gun to settle at one go, swift and convenient to operate.
(5) also to have required equipment simple and easy in the present invention, easily obtains, makes simple, economical rationality; Intubate is not needed in instillation process, simple to operate, easily grasp popularization; Rat adopts iron stand naturally to hang, and decreases the misery of rat with uncomfortable.
Accompanying drawing explanation
Fig. 1 is the anesthesia tank used in embodiment of the present invention 1-5.
Fig. 2 is the liquid-transfering gun and rifle head that use in embodiment of the present invention 1-5.
Fig. 3 is the iron stand used in embodiment of the present invention 1-5.
Detailed description of the invention
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.If do not specialize, the conventional means that technological means used in embodiment is well known to those skilled in the art, is raw materials usedly commercial goods.
Embodiment 1 is quick non-invasive rat drip-injection method precisely
1, materials and methods
1.1 material
Iron stand, liquid-transfering gun, rifle head, children's's laryngoscope, cord, anesthesia tank, absolute ether, Cotton Gossypii, timer.
Male and healthy SD rat 25, the scale of construction is 230-260g, (SPF level, Dong Chuan Animal Science service department provides by Changsha Kaifu District, credit number: SCXK (Hunan) 2009-0012).Laboratory animal divides cage to feed, and indoor temperature remains on 22 ± 2 DEG C, keeps good ventilation, within every 2 days, changes bedding and padding 1 time, carry out disinfection weekly with Bath disinfectant solution, and every 2 weeks cleaning cage tools once.
1.2 rat instillation operational approach
1.2.1 the making of tank is anaesthetized: in this test, select a transparent plastic can as anesthesia tank.Cotton Gossypii is put into tank, anesthesia pot bottom is covered, appropriate absolute ether is injected tank.
1.2.2 the making of simplified operating table: get iron stand one, can bear the cord of rat weight by one, iron clamp system, raise iron clamp height, opens to the rat tensible that front tooth can be made to hang on rope.
1.2.3 rat anesthesia: capture the rat for instiling, fast sealing is in anesthesia tank, cover tightly anesthesia tank, rat is anaesthetized in 5-10s, and anesthesia standard is motionless for falling down, and breathes slower, seemingly to sleep soundly shape, get from tank except rat fast, upper for rat front tooth is hung on the cord on an iron stand, makes it naturally hang.
1.2.4 trachea is located: hung to iron stand cord by anesthetized rat, Rat Tongue head-clamp is lived outside the oral cavity of pull-out by one people's right hand tweezers of bag one deck mull, forward tongue is pulled out gently with left hand thumb, forefinger, the right hand puts down tweezers, irradiate along the root of the tongue from oral cavity by children's's laryngoscope, clearly can see that the glottis of rat is opened along with breathing and closes as fish mouth.
1.2.5 drug drip: another people's left hand pinches rat nose, rat mouth is breathed, one one, visible glottis closes, the right hand holds the liquid-transfering gun quick insertion glottis having inhaled instillation medicine 100 μ l, by drug drip to trachea, exits liquid-transfering gun, left hand continues to pinch nose 6s, make it pass through to breathe initiatively by instillation medicine suction pulmonary, if hear moist rales, then illustrate and instil successfully.
1.2.6 medicaments uniformity distribution: rat continues to be suspended on 8s on cord, slowly rotates rat, make medicaments uniformity be distributed to rat lung after instiling and terminating.
2 results and analysis
2.1 instillation effect analyses
This test rat amounts to 25, and instillation medicine is suspension, and instillation dosage is 100 μ l/, instillation time average 28.6s consuming time, whole modeling process, from capturing rat, anaesthetizing, being instilled into the completely clear-headed 78.4s consuming time of rat, is observed after one day, instillation rat phenomenon without exception, success rate 100%.
In this instillation process, remain consummate not in the linking of links, slightly aobvious jerky, therefore, whole flow process is consuming time on average reaches 78.4s to clear-headed from capturing, anaesthetizing, instil, be uniformly distributed medicine for every rat, by loose test operation in a large number, operator's technology maturation, proficiency in action, reaction fast, tacit cooperation when, the whole modeling time can improve greatly, completely may within foreshortening to 60s.
Embodiment 2 is quick non-invasive rat drip-injection method precisely
1, materials and methods
1.1 material
Iron stand, liquid-transfering gun, rifle head, children's's laryngoscope, cord, anesthesia tank, absolute ether, Cotton Gossypii, timer
Male and healthy SD rat 25, the scale of construction is 245-290g, (SPF level, Dong Chuan Animal Science service department provides by Changsha Kaifu District, credit number: SCXK (Hunan) 2009-0012).Laboratory animal divides cage to feed, and indoor temperature remains on 22 ± 2 DEG C, keeps good ventilation, within every 2 days, changes bedding and padding 1 time, carry out disinfection weekly with Bath disinfectant solution, and every 2 weeks cleaning cage tools once.
1.2 rat instillation operational approach
1.2.1 the making of tank is anaesthetized: in this test, select a transparent plastic can as anesthesia tank.Cotton Gossypii is put into tank, anesthesia pot bottom is covered, appropriate absolute ether is injected tank.
1.2.2 the making of simplified operating table: get iron stand one, can bear the cord of rat weight by one, iron clamp system, raise iron clamp height, opens to the rat tensible that front tooth can be made to hang on rope.
1.2.3 rat anesthesia: capture the rat for instiling, fast sealing is in anesthesia tank, cover tightly anesthesia tank, rat is anaesthetized in 5-10s, and anesthesia standard is motionless for falling down, and breathes slower, seemingly to sleep soundly shape, get from tank except rat fast, upper for rat front tooth is hung on the cord on an iron stand, makes it naturally hang.
1.2.4 trachea is located: hung to iron stand cord by anesthetized rat, Rat Tongue head-clamp is lived outside the oral cavity of pull-out by one people's right hand tweezers of bag one deck mull, forward tongue is pulled out gently with left hand thumb, forefinger, the right hand puts down tweezers, irradiate along the root of the tongue from oral cavity by children's's laryngoscope, clearly can see that the glottis of rat is opened along with breathing and closes as fish mouth.
1.2.5 drug drip: another people's left hand pinches rat nose, rat mouth is breathed, one one, visible glottis closes, the right hand holds the liquid-transfering gun quick insertion glottis having inhaled instillation medicine 200 μ l, by drug drip to trachea, exits liquid-transfering gun, left hand continues to pinch nose 6s, make it pass through to breathe initiatively by instillation medicine suction pulmonary, if hear moist rales, then illustrate and instil successfully.
1.2.6 medicaments uniformity distribution: rat continues to be suspended on 8s on cord, slowly rotates rat, make medicaments uniformity be distributed to rat lung after instiling and terminating.
2 results and analysis
2.1 instillation effect analyses
This test rat amounts to 25, and instillation medicine is suspension, and instillation dosage is 200 μ l/, and instillation time average 24.7s consuming time, observes after one day, instillation rat phenomenon without exception, success rate 100%.
This modeling is carried out for the 3rd day after first time modeling, in instillation process, operator's technology is comparatively consummate, action is comparatively skilled, reaction is quick, tacit cooperation, therefore, every rat shortens about 10s from capturing, anaesthetizing, instil, be uniformly distributed medicine to clear-headed whole flow process average specific first time consuming time, is 68.4s.
Embodiment 3 is quick non-invasive rat drip-injection method precisely
1, materials and methods
1.1 material
Iron stand, liquid-transfering gun, rifle head, children's's laryngoscope, cord, anesthesia tank, absolute ether, Cotton Gossypii, timer
Male and healthy SD rat 25, the scale of construction is 265-326g, (SPF level, Dong Chuan Animal Science service department provides by Changsha Kaifu District, credit number: SCXK (Hunan) 2009-0012).Laboratory animal divides cage to feed, and indoor temperature remains on 22 ± 2 DEG C, keeps good ventilation, within every 2 days, changes bedding and padding 1 time, carry out disinfection weekly with Bath disinfectant solution, and every 2 weeks cleaning cage tools once.
1.2 rat instillation operational approach
1.2.1 the making of tank is anaesthetized: in this test, select a transparent plastic can as anesthesia tank.Cotton Gossypii is put into tank, anesthesia pot bottom is covered, appropriate absolute ether is injected tank.
1.2.2 the making of simplified operating table: get iron stand one, can bear the cord of rat weight by one, iron clamp system, raise iron clamp height, opens to the rat tensible that front tooth can be made to hang on rope.
1.2.3 rat anesthesia: capture the rat for instiling, fast sealing is in anesthesia tank, cover tightly anesthesia tank, rat is anaesthetized in 5-10s, and anesthesia standard is motionless for falling down, and breathes slower, seemingly to sleep soundly shape, get from tank except rat fast, upper for rat front tooth is hung on the cord on an iron stand, makes it naturally hang.
1.2.4 trachea is located: hung to iron stand cord by anesthetized rat, Rat Tongue head-clamp is lived outside the oral cavity of pull-out by one people's right hand tweezers of bag one deck mull, forward tongue is pulled out gently with left hand thumb, forefinger, the right hand puts down tweezers, irradiate along the root of the tongue from oral cavity by children's's laryngoscope, clearly can see that the glottis of rat is opened along with breathing and closes as fish mouth.
1.2.5 drug drip: another people's left hand pinches rat nose, rat mouth is breathed, one one, visible glottis closes, the right hand holds the liquid-transfering gun quick insertion glottis having inhaled instillation medicine 300 μ l, by drug drip to trachea, exits liquid-transfering gun, left hand continues to pinch nose 6s, make it pass through to breathe initiatively by instillation medicine suction pulmonary, if hear moist rales, then illustrate and instil successfully.
1.2.6 medicaments uniformity distribution: rat continues to be suspended on 8s on cord, slowly rotates rat, make medicaments uniformity be distributed to rat lung after instiling and terminating.
2 results and analysis
2.1 instillation effect analyses
This test rat amounts to 25, instillation medicine is suspension, instillation dosage is 300 μ l/, instillation time average 22.5s consuming time, whole modeling process is from capturing rat, anaesthetizing, being instilled into the completely clear-headed 58.2s consuming time of rat, and rats death 1 in instillation process, observed after one day, all the other rats phenomenon without exception, success rate 96.0%.
This modeling is carried out for the 3rd day after second time modeling, in instillation process, operator is skilled, proficiency in action, reaction fast, tacit cooperation, rats death reason is likely that the dosage that once instils is too large, rats breathing is short of power, and suffocates and dies, therefore, the drug drip dosage range be applicable to for finding the present invention, test further of still needing.
Embodiment 4 is quick non-invasive rat drip-injection method precisely
1, materials and methods
1.1 material
Iron stand, liquid-transfering gun, rifle head, children's's laryngoscope, cord, anesthesia tank, absolute ether, Cotton Gossypii, timer
Male and healthy SD rat 25, the scale of construction is 285-345g, (SPF level, Dong Chuan Animal Science service department provides by Changsha Kaifu District, credit number: SCXK (Hunan) 2009-0012).Laboratory animal divides cage to feed, and indoor temperature remains on 22 ± 2 DEG C, keeps good ventilation, within every 2 days, changes bedding and padding 1 time, carry out disinfection weekly with Bath disinfectant solution, and every 2 weeks cleaning cage tools once.
1.2 rat instillation operational approach
1.2.1 the making of tank is anaesthetized: in this test, select a transparent plastic can as anesthesia tank.Cotton Gossypii is put into tank, anesthesia pot bottom is covered, appropriate absolute ether is injected tank.
1.2.2 the making of simplified operating table: get iron stand one, can bear the cord of rat weight by one, iron clamp system, raise iron clamp height, opens to the rat tensible that front tooth can be made to hang on rope.
1.2.3 rat anesthesia: capture the rat for instiling, fast sealing is in anesthesia tank, cover tightly anesthesia tank, rat is anaesthetized in 5-10s, and anesthesia standard is motionless for falling down, and breathes slower, seemingly to sleep soundly shape, get from tank except rat fast, upper for rat front tooth is hung on the cord on an iron stand, makes it naturally hang.
1.2.4 trachea is located: hung to iron stand cord by anesthetized rat, Rat Tongue head-clamp is lived outside the oral cavity of pull-out by one people's right hand tweezers of bag one deck mull, forward tongue is pulled out gently with left hand thumb, forefinger, the right hand puts down tweezers, irradiate along the root of the tongue from oral cavity by children's's laryngoscope, clearly can see that the glottis of rat is opened along with breathing and closes as fish mouth.
1.2.5 drug drip: another people's left hand pinches rat nose, rat mouth is breathed, one one, visible glottis closes, the right hand holds the liquid-transfering gun quick insertion glottis having inhaled instillation medicine 400 μ l, by drug drip to trachea, exits liquid-transfering gun, left hand continues to pinch nose 6s, make it pass through to breathe initiatively by instillation medicine suction pulmonary, if hear moist rales, then illustrate and instil successfully.
1.2.6 medicaments uniformity distribution: rat continues to be suspended on 8s on cord, slowly rotates rat, make medicaments uniformity be distributed to rat lung after instiling and terminating.
2 results and analysis
2.1 instillation effect analyses
This test rat amounts to 24, instillation medicine is suspension, instillation dosage is 400 μ l/, instillation time average 23.8s consuming time, whole modeling process is from capturing rat, anaesthetizing, being instilled into the completely clear-headed 61.4s consuming time of rat, and rats death 3 in instillation process, observed after one day, all the other rats phenomenon without exception, success rate 87.5%.
This modeling is carried out for the 3rd day after third time modeling, in instillation process, operator is skilled, proficiency in action, reaction fast, tacit cooperation, but, instil relative to third time, this consumes the increase of note time, and main cause is that dropped amount increases, for avoiding rat to suffocate, drip velocity slows down to some extent; In addition, compare first three instillation, this rats death 3, mortality rate increases, and instil before summary experience, and be speculated as the disposable instillation dosage of rat too large, dyspnea, suffocates and die.For verifying that whether this supposition is correct further, in next one test, 400 μ l medicines can be instiled at twice, finally comparing instillation effect and mortality rate.
Embodiment 5 is quick non-invasive rat drip-injection method precisely
1, materials and methods
1.1 material
Iron stand, liquid-transfering gun, rifle head, children's's laryngoscope, cord, anesthesia tank, absolute ether, Cotton Gossypii, timer
Male and healthy SD rat 25, the scale of construction is 305-365g, (SPF level, Dong Chuan Animal Science service department provides by Changsha Kaifu District, credit number: SCXK (Hunan) 2009-0012).Laboratory animal divides cage to feed, and indoor temperature remains on 22 ± 2 DEG C, keeps good ventilation, within every 2 days, changes bedding and padding 1 time, carry out disinfection weekly with Bath disinfectant solution, and every 2 weeks cleaning cage tools once.
1.2 rat instillation operational approach
1.2.1 the making of tank is anaesthetized: in this test, select a transparent plastic can as anesthesia tank.Cotton Gossypii is put into tank, anesthesia pot bottom is covered, appropriate absolute ether is injected tank.
1.2.2 the making of simplified operating table: get iron stand one, can bear the cord of rat weight by one, iron clamp system, raise iron clamp height, opens to the rat tensible that front tooth can be made to hang on rope.
1.2.3 rat anesthesia: capture the rat for instiling, fast sealing is in anesthesia tank, cover tightly anesthesia tank, rat is anaesthetized in 5-10s, and anesthesia standard is motionless for falling down, and breathes slower, seemingly to sleep soundly shape, get from tank except rat fast, upper for rat front tooth is hung on the cord on an iron stand, makes it naturally hang.
1.2.4 trachea is located: hung to iron stand cord by anesthetized rat, Rat Tongue head-clamp is lived outside the oral cavity of pull-out by one people's right hand tweezers of bag one deck mull, forward tongue is pulled out gently with left hand thumb, forefinger, the right hand puts down tweezers, irradiate along the root of the tongue from oral cavity by children's's laryngoscope, clearly can see that the glottis of rat is opened along with breathing and closes as fish mouth.
1.2.5 drug drip: another people's left hand pinches rat nose, rat mouth is breathed, one one, visible glottis closes, the right hand holds the liquid-transfering gun quick insertion glottis having inhaled instillation medicine 400 μ l, is slowly instiled to trachea at twice by medicine, exits liquid-transfering gun, left hand continues to pinch nose 8s, make it pass through to breathe initiatively by instillation medicine suction pulmonary, if hear moist rales, then illustrate and instil successfully.
1.2.6 medicaments uniformity distribution: rat continues to be suspended on 8s on cord, slowly rotates rat, make medicaments uniformity be distributed to rat lung after instiling and terminating.
2 results and analysis
2.1 instillation effect analyses
This test rat amounts to 21, and instillation medicine is suspension, instillation dosage be 400 μ l/ only, once suck 400 μ l and to instil at twice a rat trachea.Instillation time average 27.3s consuming time, whole modeling process, from capturing rat, anaesthetizing, being instilled into the completely clear-headed 66.7s consuming time of rat, without rats death in instillation process, is observed after one day, all the other rats phenomenon without exception, success rate 100%.
This modeling is carried out for the 3rd day after the 4th modeling, in instillation process, operator is skilled, proficiency in action, reaction fast, tacit cooperation, instillation dosage is similarly 400 μ l, be divided into twice, slowly inject a rat trachea, the no asphyxia phenomena of mortality in instillation, second day phenomenon without exception, success rate 100%.Because dosage increases, instillation gradation and slowly, the instillation time compare second time, for the third time, the 4th time lengthening, but ensure that success rate.
By above embodiment, can determine that the present invention instils dosage at 300 below μ l, can once instil, safe and efficient, dropped amount, at 300 more than μ l, is divided into and slowly instils for twice, can guarantee the success rate of rat equally.
In addition, in instillation process, note also following item: the first, must the motion of close observation animal and breathing state during anesthesia, prevent from anaesthetizing dark or excessively shallow; The second, carry out preparation of instiling before anesthesia, make anesthesia and instillation operation accomplish slitless connection, raise the efficiency; Three, prepare the cotton mass being moistened with ether in advance, in case rat revives in instiling, if revived, rapidly cotton mass is blocked rat nostril, make it continue anesthesia, ensure to instil and carry out smoothly.
Fig. 1-Fig. 3 is shown in respectively by the schematic diagram of the anesthesia tank used in embodiment 1-5, liquid-transfering gun and rifle head and iron stand.
Test rat dropped amount, instillation time and Success Rate Analysis in embodiment 1-5 and the results are shown in Table 1.
Table 1 tests rat dropped amount, instillation time and Success Rate Analysis
| Project | Dropped amount (μ l) | Instillation time (s) | Modeling time (s) | Instillation quantity (only) | Success rate (%) |
| Embodiment one | 100 | 28.6 | 78.4 | 25 | 100.0 |
| Embodiment two | 200 | 24.7 | 65.1 | 25 | 100.0 |
| Embodiment three | 300 | 22.5 | 58.2 | 25 | 96.0 |
| Embodiment four | 400 once instil | 23.8 | 61.4 | 24 | 87.5 |
| Embodiment five | 400 twice instillation | 26.3 | 66.7 | 21 | 100.0 |
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (6)
1. an accurate non-invasive rat drip-injection method fast, it is characterized in that, described method is after rat etherization, is suspended on iron stand, lives outside pull-out oral cavity with the tweezers of looping gauze by Rat Tongue head-clamp, glottis is found under laryngoscope irradiates, then pinch rat nose, rat mouth is breathed, along with glottis folding, to inhale the liquid-transfering gun quick insertion glottis of instillation medicine, by drug drip to trachea; Exit liquid-transfering gun, continue to pinch nose 3-8s, make it pass through to breathe automatically by instillation medicine suction pulmonary, moist rales to be heard, shows to instil successfully.
2. method according to claim 1, is characterized in that, instils after terminating, rat is continued to be suspended on 5-10s on iron stand, keeps health naturally to hang, slowly rotates rat, make medicaments uniformity be distributed to rat lung.
3. method according to claim 1, is characterized in that, rat is placed in anesthesia tank with cover and anaesthetizes, and is placed with the Cotton Gossypii or gauze of dipping in full ether in anesthesia tank.
4. method according to claim 3, is characterized in that, described anesthesia tank is glass jar or transparent plastic can.
5. method according to claim 1, is characterized in that, after rat etherization, hitches rat front tooth, be suspended on iron stand with cord.
6. the method according to any one of claim 1-5, is characterized in that, each instillation dosage control is within 300 μ l.
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| CN201510847643.4A CN105434081A (en) | 2015-11-27 | 2015-11-27 | Accurate, quick and noninvasive method for instilling medicine into rat |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107174370A (en) * | 2017-07-05 | 2017-09-19 | 海南医学院 | A kind of experimental rat holder for suction-type tracheal instillation |
| CN109893289A (en) * | 2017-12-08 | 2019-06-18 | 李莎 | A kind of noninvasive intrarterial device and medication applied to experiment mice |
| CN111281598A (en) * | 2020-03-05 | 2020-06-16 | 纳菲(深圳)制药科技有限公司 | Anesthesia control system and method |
| CN112674031A (en) * | 2020-12-03 | 2021-04-20 | 同济大学 | Rodent model infected by novel coronavirus SARS-CoV-2 and construction method and use thereof |
-
2015
- 2015-11-27 CN CN201510847643.4A patent/CN105434081A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107174370A (en) * | 2017-07-05 | 2017-09-19 | 海南医学院 | A kind of experimental rat holder for suction-type tracheal instillation |
| CN109893289A (en) * | 2017-12-08 | 2019-06-18 | 李莎 | A kind of noninvasive intrarterial device and medication applied to experiment mice |
| CN111281598A (en) * | 2020-03-05 | 2020-06-16 | 纳菲(深圳)制药科技有限公司 | Anesthesia control system and method |
| CN111281598B (en) * | 2020-03-05 | 2023-12-08 | 纳菲(深圳)制药科技有限公司 | Anesthesia control system and method |
| CN112674031A (en) * | 2020-12-03 | 2021-04-20 | 同济大学 | Rodent model infected by novel coronavirus SARS-CoV-2 and construction method and use thereof |
| CN112674031B (en) * | 2020-12-03 | 2021-11-30 | 同济大学 | Construction method and application of novel coronavirus-infected rodent model |
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Application publication date: 20160330 |