CN110521965A - A kind of hippocampus glue and preparation method thereof - Google Patents
A kind of hippocampus glue and preparation method thereof Download PDFInfo
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- CN110521965A CN110521965A CN201910873196.8A CN201910873196A CN110521965A CN 110521965 A CN110521965 A CN 110521965A CN 201910873196 A CN201910873196 A CN 201910873196A CN 110521965 A CN110521965 A CN 110521965A
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- hippocampus
- glue
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/20—Fish extracts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/60—Fish, e.g. seahorses; Fish eggs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses a kind of preparation methods of hippocampus glue, it includes the following steps: 1) to take hippocampus, crush, are wrapped up with filter bag, add water infusion, filter, and take filtrate that plastic is concentrated；2) take step 1) colloid to be condensed into medicinal extract, sequentially add yellow rice wine, sugar and edible oil mix, it is dry to get.The preparation method of hippocampus glue of the present invention is wrapped up raw material with filter bag, is mixed by adding water infusion 2 times, then with special ratios auxiliary material, and total amino acid content is significantly larger than raw material in hippocampus glue obtained, and has high antioxidant activity.The production method of hippocampus glue of the present invention is simple, has practical application promotional value.Hippocampus glue of the invention provides a kind of new selection as a kind of dosage form that nutritional ingredient is richer, for consumer.
Description
Technical field
Present invention relates particularly to a kind of hippocampus glue and preparation method thereof
Background technique
Jelly means that skin, bone, first or the angle of animal take colloid with decocting, is condensed into thick glue shape, manufactured solid after drying
Body bulk oral preparations.The glue technology of Ancient Times in China is tempered extraction basically by raw material and is concentrated again, with science and technology
It continues to develop, present adhesive-preparing technology is fairly advanced, finally various pieces of shape dry packings is made more and forms.In recent years, in original
In terms of expecting jelly, the new products such as new donkey-hide gelatin, antler gelatin, Chelonia mydas (Linnaeus) are succeeded in developing, but have prepared work about hippocampus glue not yet
The research of skill and the report of hippocampus glue product.
Chinese medicine hippocampus is that sea is dwelt fish Syngnathidae animal strain line hippocampus Hippocampus kelloggi Jordan et
Snyder, hippocampus Hippocampus histrix Kaup, Hippocampus Kuda Hippocampus kuda Bleeker, hippocampus trimaculatus Leacs
The drying of Hippocampus trimaculatus Leach or small hippocampus (extra large maggot) Hippocampus japonicus Kaup
Body.Its nature and flavor is sweet, salty, temperature；Return liver and kidney channel.With warming kidney and enhancing body, the effect of mass dissipating and swelling eliminating.For impotence, the enuresis, kidney deficiency is made
Chuan , lumps in the chest and abdomen, injury from falling down；Controlling outward carbuncle swells furunculosis.The raw medicinal animal in the precious sea of hippocampus system.In the past, medicinal hippocampus was next
Source relies primarily on wild capture, and yield is very low, is far from satisfying the needs prevented and cured diseases.In recent years, with cause of science day
The different development of crescent, the artificial breeding of hippocampus have been succeeded.This is the research and extensive clinical application of hippocampus medicinal component,
Provide extremely advantageous condition.In recent years, hippocampus had been processed to anther sac or tablet, further improved curative effect, but extra large
In process, part effective component and nutritional ingredient lost horse during the preparation process, and effective component cannot be complete
All risk insurance stays.It is some using hippocampus as the food of raw material or health care product complicated component therein, it is distinctive that hippocampus cannot be embodied completely
Medical value.
Summary of the invention
To solve the above problems, the present invention provides a kind of preparation method of hippocampus glue, it is to include the following steps:
1) hippocampus is taken, crushes, is wrapped up with filter bag, adds water logging to float 24-36h, adds water infusion, filters, obtains filtrate；
2) take step 1) filtrate, sugaring is condensed into medicinal extract, then plus wine and edible oil mix, it is dry to get.
Further, grain diameter is not less than 2.0mm after the step 1) crushing.
Further, the step 1) soaking time is for 24 hours；The infusion is to add 30 times of amount (v/w；Ml/g water), infusion 2
It is secondary, 3 hours every time.
Further, the step 1) filtrate is that latter incorporated filtrate is filtered in infusion twice.
Further, the additional amount of the step 2) sugar is the 1-5% (w/w of hippocampus weight；g/g)；The addition of the wine
Amount is the 1-5% (v/w of hippocampus weight；ml/g)；The additional amount of the edible oil is the 1-5% (w/w of hippocampus weight；g/g).
Further, the additional amount of the sugar is 2% (w/w of hippocampus weight；g/g)；The additional amount of the wine is hippocampus
3% (v/w of weight；ml/g)；The additional amount of the edible oil is 1.2% (w/w of hippocampus weight；g/g).
Further, the sugar is rock sugar；The edible oil is sesame oil；The wine is yellow rice wine.
Further, the every 1g 2.6g containing hippocampus of medicinal extract described in step 2).
Further, the method for the step 2) drying is to dry or low temperature drying.
Further, the temperature of the low temperature drying is lower than 70 DEG C, preferably 50~70 DEG C.
The present invention also provides a kind of hippocampus glue of aforementioned preparation process preparation, its total amino acid content is greater than 60%.
The present invention provides a kind of preparation method of hippocampus glue, raw material is wrapped up with filter bag, by adding water infusion 2 times, then with
The mixing of special ratios auxiliary material, hippocampus glue fishy smell obtained is small, and total amino acid content is significantly larger than raw material, and has high anti-oxidant work
Property.Production method is also simple, has practical application promotional value, and hippocampus glue of the invention is richer as a kind of nutritional ingredient
Dosage form provides a kind of new selection for consumer.
Obviously, above content according to the present invention is not being departed from according to the ordinary technical knowledge and customary means of this field
Under the premise of the above-mentioned basic fundamental thought of the present invention, the modification, replacement or change of other diversified forms can also be made.
The specific embodiment of form by the following examples remakes further specifically above content of the invention
It is bright.But the range that this should not be interpreted as to the above-mentioned theme of the present invention is only limitted to example below.It is all to be based on above content of the present invention
The technology realized all belongs to the scope of the present invention.
Detailed description of the invention
Clearance rate of Fig. 1 hippocampus glue sample to DPPH free radical
Clearance rate of Fig. 2 BHT to DPPH free radical
Specific embodiment
The preparation of embodiment 1, hippocampus glue of the present invention
1) hippocampus 20g is taken, the particle that partial size is not less than 2.0mm is ground into, is wrapped up with filter bag, water logging drift is added to be put into afterwards for 24 hours
In pot, adding 600ml water, infusion 3 hours, filtrate was poured out in filtering, by the carry out infusion of filter residue plus 600ml water again 3 hours,
Filtering merges filtrate twice；
2) step 1) filtrate is taken, 0.4g candy sugar powder is added, then is condensed into medicinal extract (every 1g medicinal extract contains raw material 2.6g), is added
0.6ml yellow rice wine, 0.24g sesame oil mix, and inject silica gel mould, sunning to dry, demoulding to get.
The preparation of embodiment 2, hippocampus glue of the present invention
1) hippocampus 20g is taken, the particle that partial size is not less than 2.0mm is ground into, is wrapped up with filter bag, water logging drift is added to be put into afterwards for 24 hours
In pot, adding 600ml water, infusion 3 hours, filtrate was poured out in filtering, by the carry out infusion of filter residue plus 600ml water again 3 hours,
Filtering merges filtrate twice；
2) step 1) filtrate is taken, 0.2g candy sugar powder is added, then be condensed into medicinal extract (every g medicinal extract raw material 2.6g containing hippocampus), then
0.4ml yellow rice wine is added, 0.2g sesame oil mixes, inject silica gel mould, sunning to dry, demoulding to get.
The preparation of embodiment 3, hippocampus glue of the present invention
1) hippocampus 20g is taken, the particle that partial size is not less than 2.0mm is ground into, is wrapped up with filter bag, is put into after adding water logging to float 36h
In pot, adding 600ml water, infusion 3 hours, filtrate was poured out in filtering, by the carry out infusion of filter residue plus 600ml water again 3 hours,
Filtering merges filtrate twice；
2) step 1) filtrate is taken, 0.6g candy sugar powder is added, then is condensed into medicinal extract (every g medicinal extract contains raw material 2.6g), is added
0.8ml yellow rice wine, 0.28g sesame oil mix, and inject silica gel mould, sunning to dry, demoulding to get.
Illustrate beneficial effects of the present invention below by way of test example.
Test example 1
1.1 reagents: distilled water, yellow rice wine, sesame oil, white sugar, hippocampus powder, whole hippocampus
1.2 instrument and equipments: filter bag, beaker, glass bar, evaporating dish, iron stand, condenser pipe, round-bottomed flask, electronic balance,
Pressure cooker, centrifuge, baking oven
2 glue processes
The preparation process flow of jelly are as follows: the selection of supplementary material and processing → take glue juice (enduring glue) → filtration clarification → dense
Contracting receives glue → gel and cuts glue → drying → quality examination → packaging.
3 preparation processes screening
3.1 endure peptizing agent
It weighs hippocampus powder or is weighed after shredding whole hippocampus, wrapped up with filter bag, water is added or yellow rice wine carries out
Infusion carries out infusion with different time and mode, is filtered and is concentrated and receive glue, the glue being concentrated is placed in evaporating dish and is put
Enter be put into drier after (105 DEG C, 3 hours) of baking oven drying it is cooling after weigh, calculate dry cream rate (dried object weight * 100%/
Raw material weight), it the results are shown in Table 1.
1 dry cream rate of table
Note: result has deducted the paste-forming rate of yellow rice wine in table.
Infusion solvent is water as seen from Table 1, and the additional amount of water is 30 times of amounts (v/w, ml/g), and the infusion time is 3 hours, is endured
Boiling number is two suboptimums.And leaching drift process should prevent powder leakage from losing as can be known from the above table, and hippocampus powder can not too thin (sieve mesh number
No more than 10 meshes, 10 mesh mesh screen partial size 2.0mm), it can shred.
The auxiliary material being added during 3.2 glues
It extracted according to screening technology, clean, be concentrated, receive glue.The liquid that 3.1 best infusion modes obtain is carried out
Concentration, when beginning, are proportionally added into candy sugar powder, the sesame oil being added at thick medicinal extract (when hanging flag or every g medicinal extract contains raw material 2.6g)
And yellow rice wine, after being slightly concentrated, it is then transferred to natural cooling in food grade silicone mold and forms, it is dry, compare the aesthetic appearance of finished product
Shape, mouldability etc..It the results are shown in Table 2.
Auxiliary material situation is added in table 2
Note: supplementary product consumption is the percentage that auxiliary material accounts for raw material, and "-" expression is not added.
Raw material glue saline taste after rinsing is thin, and the hippocampus raw material in experiment is by leaching drift processing.It can by table 2
Know, oily ratio is arranged more than 3%, and the drying and moulding time extends, and finished surface has oil stain, there is sesame oil taste.Suitable control addition Icing Sugar,
Yellow rice wine, sesame oil can achieve flavoring and rectify smelly effect, cover the fishy smell of hippocampus.When the additional amount of sugar is the 2% of hippocampus weight
(w/w；G/g), the additional amount of yellow rice wine is 3% (v/w of hippocampus weight；Ml/g), the additional amount of sesame oil is the 1.2% of hippocampus weight
(w/w；When g/g), the character of finished product is brown, and matter is hard, crisp；Fishy smell is light.
Ingredient in 3.4 hippocampus
Modern research shows that hippocampus nutritive and medicinal value with higher and a variety of chemical active ingredients.Change in hippocampus
Study point many kinds of, studying more chemical component includes amino acid, steroid, fatty acid, microelement and phosphatide etc., this
A little effective components are the important chemical fundamentals that hippocampus plays pharmacological action.According to amino acid in GB5009.124-2016 food
Measuring method is respectively raw material according to embodiment 1 to hippocampus trimaculatus Leacs and using it, but does not add hippocampus glue made of auxiliary material and surveyed
It is fixed.It the results are shown in Table 3.
3 amino acid testing result of table
By 16 kinds of amino acid contents in 3 raw material hippocampus of table and its jelly it is found that glycine, glutamic acid in raw material, third
Histidine content is higher；Glycine, alanine, proline in jelly, the content of glutamic acid are higher, and together with arginine, this is several
Kind amino acid is above the content in raw material, and tyrosine, leucine, isoleucine content are lower than the content in raw material；It is made
After jelly, total amino acid content is higher compared with material content.
The antioxidation in vitro of 3.5 hippocampus glue is tested
Free radical caused by body oxidation reaction has strong oxidizing property, many chronic diseases and aging effect with human body
It is all closely related.Oxidative stress can damage the tissue and cell of body, and blood vessel can be made by damage in a way, thus
Cause the cardiovascular diseases such as hypertension.By the chemical composition analysis to hippocampus, sterols rich in hippocampus is found
Closing ingredients, the hypoxanthine such as object, a variety of fatty acid, protein, amino acid, microelement, phospholipid is the main antioxygen of hippocampus
Change active material.To the hippocampus glue prepared according to embodiment 1, antioxidant activity external test is carried out, including to DPPH free radical
The measurement of clearance rate, the experiment such as the measurement of hydroxyl radical free radical clearance rate and reducing power measurement.
(1) measurement of DPPH free radical scavenging activity
DPPH free radical is a kind of artificial synthesized, stable organic free radical, and methanol or ethanol solution are in prune
Color has a strong absworption peak at 517nm.Antioxidant and its lone pair electrons match, and absorption peak can fade away, darkviolet
DPPH free radical is reduced into yellow DPPH-H non-free radical form, and fading extent and the electron amount received are at quantitative pass
System can carry out quantitative analysis by the variation of absorbance.
DPPH free radical scavenging activity=(A0-A1)/A0 × 100%.
A1: sample sets absorbance value；A0: control group absorbance value；
According to the clearance rate and corresponding sample additive amount, the linear regression song that sample removes DPPH free radical can be drawn
The concentration to hippocampus glue when DPPH free radical scavenging activity is 50%, i.e. IC is calculated according to regression equation in line50.In order to sea
The oxidation resistance of horse glue is intuitively evaluated, and obtains the IC of positive control using same method50, and the two is compared.
Respectively into test tube be added 0.08ml, 0.12ml, 0.16ml, 0.20ml, 0.24ml, 0.28ml, 0.32ml,
0.36ml, 0.40ml concentration are the hippocampus glue sample solution of 10mg/ml, add purified water to 2ml, then be separately added into 2ml
0.06mg/ml DPPH solution is vortexed and mixes, avoid light place 2.5 hours, with the hippocampus glue sample solution of respective concentration, adds pure
Change water to 2ml, and 2ml dehydrated alcohol is added, operated with method, as blank control, absorbance, record survey are measured 517nm at
Definite value A1.
Take 2ml 0.06mg/ml DPPH solution and 2ml purified water in test tube, avoid light place 45min uses dehydrated alcohol
As blank control, its absorbance at 517nm is surveyed, records measured value A0.
Be 0.005mg/ml, 0.010mg/ml with concentration gradient, 0.015mg/ml, 0.020mg/ml, 0.025mg/ml,
The BHT of 0.030mg/ml, 0.035mg/ml, 0.040mg/ml are measured with sample treatment same operation, as positive control.
It the results are shown in Table 4 and Fig. 1, after each concentration gradient hippocampus sol solution about 150min is added in DPPH solution, reaction is basic
Completely.Sample is y=30.66x+12.73, R to the measurement result regression equation of the clearance rate of DPPH free radical2=0.990.
Clearance rate of the 4 hippocampus sample of table to DPPH free radical
By calculating the hippocampus glue sample concentration IC acquired when elimination factor is 50%50=1.215mg/ml.
After each concentration gradient BHT solution about 120min is added in DPPH solution, reaction is substantially completely.BHT is to DPPH freedom
The clearance rate of base the results are shown in Table 5 and Fig. 2.
Clearance rate of 5 BHT of table to DPPH free radical
BHT can be obtained to the IC when clearance rate of DPPH is 50% according to fig. 250About 0.02mg/ml.
(2) measurement of hydroxyl radical free radical (OH) clearance rate
Hydroxyl radical free radical (OH) property is very active, is known strongest oxidant, makees to cell and disorganization
With maximum.Hydroxyl radical free radical can be generated by reaction, H2O2+Fe2+=OH+OH+Fe3+, the OH time-to-live is short, is reacting
Salicylic acid is added in system, can effectively capture OH, generates color products, there is strong absorption, light absorption value at 510nm wavelength
It is directly proportional to the amount of OH.If being added in reaction system has the substance for removing OH function, OH can be competed with salicylic acid, made
The production quantity of color products is reduced, and using fixed reaction time methods, the comparable antioxidant of light absorption value measured at 510nm is clear
Except the ability of OH.Spectral scan is carried out in 200~800nm wave-length coverage, it is found that this reaction system has maximum suction in 527nm
It receives, therefore measurement wavelength is changed to 527nm.
Hydroxyl radical free radical (OH) clearance rate=(A0-A1)/A0× 100%
A1: sample sets absorbance value；A0: control group absorbance value；
According to the clearance rate and corresponding hippocampus sample solution additive amount, hippocampus sample can be drawn and remove hydroxyl radical free radical
Linear regression curves, according to regression equation, be calculated to hydroxyl radical free radical clearance rate be 50% when Hippocampus extract it is dense
Degree, i.e. IC50.The IC of positive control is obtained using same method50, and the two is compared.
Sample sets: 0.1ml, 0.2ml, 0.4ml, 0.6ml, 0.8ml, 1.0ml, 1.2ml concentration is added into test tube respectively
For the hippocampus glue sample solution of 10mg/ml, then the FeSO of 0.3ml 9mmol/L is successively added into test tube4Solution, 0.3ml
The H of 0.3ml 8.8mmol/L is added with deionized water polishing to 7ml in salicylic acid-ethanol solution of 9mmol/L after shaking up2O2It is molten
Liquid starting reaction, in 37 DEG C of heat preservation 10min；H is replaced with deionized water2O2Solution is operated with sample sets, as blank control, is surveyed
Determine light absorption value A1 of the solution at 527nm.
Control group: hippocampus glue sample solution is replaced with deionized water, is operated with sample sets, is blank pair with deionized water
According to light absorption value A0 of the measurement solution at 527nm.
It is the glutathione (GSH) of 1mg/ml, 2mg/ml, 4mg/ml, 6mg/ml, 8mg/ml, same sample with concentration gradient
Processing method same operation measurement, as positive control.
As a result see, the absorbance value for measuring control group is 0.521, is added after sample, to the clearance rate of hydroxyl radical free radical
Measurement result be shown in Table 6, Hippocampus extract increases the Scavenging activity of hydroxyl radical free radical with increasing for concentration gradient, in good
Linear relationship, linear regression straight line equation be y=7.038x+7.694, R2=0.991, acquiring clearance rate by calculating is
Hippocampus extract concentration IC when 50%50=6.016mg/ml.
Positive control glutathione is in good linear relationship, warp in 1mg/ml to 8mg/ml to the clearance rate of hydroxyl radical free radical
Linear regression obtains linear equation y=7.34x-1.981, R2=0.996.GSH when clearance rate is 50% is acquired by calculating
Concentration is IC50=6.629mg/ml.
Clearance rate of the table 6 to hydroxyl radical free radical (OH)
(3) measurement of reducing power
The electronics that antioxidant can provide makes the Fe of the potassium ferricyanide3+It is reduced to Fe2+, generate potassium ferrocyanide, ferrocyanide
Potassium is further and ferric chloride reaction, generation have the Prussian blue (Fe of absorption maximum at 700nm4[Fe(CN)6]3), to make
Solution colour becomes different degrees of green to blue by original yellow.It can be with by the size of absorbance at measurement 700nm
The reducing power size of reflection antioxidant indirectly, absorbance is bigger, and reducing power is stronger, and antioxidant effect is better.
Hippocampus glue sample solution 0.4ml, 0.6ml, 0.8ml, 1ml and 20mg/ of 10mg/ml are added into 10ml centrifuge tube
Hippocampus glue sample solution 0.6ml, 0.7ml, 0.8ml, 0.9ml of ml, adds purified water to 1ml, sequentially adds 2.5ml mass
The potassium ferricyanide and 2.5ml phosphate buffer solution (0.2mol/L, pH=6.6) that concentration is 1% are kept the temperature at 50 DEG C after mixing
20min is then placed in 4 DEG C of 5min pause reactions of refrigerator, it is molten that the trichloroacetic acid that 2.5ml mass concentration is 10% is added after taking-up
Liquid is centrifuged 10min after mixing with 3000r/min, takes supernatant 2.5ml that 2.5ml purified water and 0.5mL mass concentration is added
For 0.7% FeCl3Solution stands 10min after mixing and surveys its absorbance at 700nm.It takes with above-mentioned concentration gradient
2.5mL phosphate buffer solution and 2.5ml purified water is added in hippocampus sample solution 1ml, after mixing in 50 DEG C of heat preservation 20min,
4 DEG C of 5min of refrigerator are then placed in, 2.5ml purified water is added after taking-up, is centrifuged 10min after mixing, supernatant 2.5ml is taken to add
Enter 3ml purified water, is measured as blank reference.
It is the paddy of 0.1mg/ml, 0.2mg/ml, 0.3mg/ml, 0.4mg/ml, 0.5mg/ml, 0.6mg/ml with concentration gradient
The sweet peptide of Guang is measured with sample treatment same operation, as positive control.
As a result, the measurement of the reducing power of sample is shown in Table 7, total reducing power of hippocampus glue gradually increases within the scope of concentration gradient
Greatly, regression equation y=0.048x+0.106, R2=0.985, there is similar reducing power with glutathione.
The measurement of 7 reducing power of table
The antioxidation in vitro experimental study of hippocampus glue shows that hippocampus glue can remove DPPH free radical, the IC of clearance rate50=
The IC of 1.215mg/ml, BHT50=0.02mg/ml；Hydroxyl radical free radical, the IC of clearance rate can significantly be removed50=6.016mg/ml,
The IC of GSH50=6.629mg/ml；Hippocampus glue has stronger reducing power.
To sum up, the preparation method of hippocampus glue of the present invention is mixed by adding water infusion 2 times, then with special ratios auxiliary material, is made
Hippocampus glue fishy smell it is small, and total amino acid content is significantly larger than raw material, and has high antioxidant activity.Production method is also simple,
Have a practical application promotional value, hippocampus glue made of the present invention as a kind of higher dosage form of nutritional ingredient, facilitate storage and
It uses, also provides a kind of richer new selection of nutrition to consumer.
Claims (10)
1. a kind of preparation method of hippocampus glue, it is characterised in that: it is to include the following steps:
1) hippocampus is taken, crushes, is wrapped up with filter bag, adds water logging to float 24-36h, adds water infusion, filters, obtains filtrate；
2) take step 1) filtrate, sugaring is condensed into medicinal extract, then plus wine and edible oil mix, it is dry to get.
2. preparation method according to claim 1, it is characterised in that: step 1) the leaching drift time is for 24 hours；The infusion is
Add 30 times of amount (v/w；Ml/g water), infusion 2 times, every time 3 hours.
3. preparation method according to claim 1, it is characterised in that: grain diameter is not less than after the step 1) crushing
2.0mm；The filtrate is that latter incorporated filtrate is filtered in infusion twice.
4. preparation method according to claim 1, it is characterised in that: the additional amount of the step 2) sugar is hippocampus weight
1-5% (w/w；g/g)；The additional amount of the wine is the 1-5% (v/w of hippocampus weight；ml/g)；The additional amount of the edible oil is
1-5% (the w/w of hippocampus weight；g/g).
5. preparation method according to claim 1, it is characterised in that: the additional amount of the sugar is 2% (w/ of hippocampus weight
w；g/g)；The additional amount of the wine is 3% (v/w of hippocampus weight；ml/g)；The additional amount of the edible oil is hippocampus weight
1.2% (w/w；g/g).
6. according to claim 1, preparation method described in 4 or 5, it is characterised in that: the sugar is rock sugar；The edible oil is fiber crops
Oil；The wine is yellow rice wine.
7. preparation method according to claim 1, it is characterised in that: the every 1g 2.6g containing hippocampus of the step 2) medicinal extract.
8. preparation method according to claim 1, it is characterised in that: the method for the step 2) drying is to dry or low temperature
Drying.
9. preparation method according to claim 8, it is characterised in that: the temperature of the low temperature drying is lower than 70 DEG C, preferably
50~70 DEG C.
10. the hippocampus glue of preparation method preparation described in any one of claim 1 to 9, which is characterized in that its amino acid is total
Content is greater than 60%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112369612A (en) * | 2020-10-12 | 2021-02-19 | 郑州轻工业大学 | Preparation method of donkey skin collagen peptide edible gum |
| CN114209718A (en) * | 2021-12-10 | 2022-03-22 | 海南医学院 | Preparation method and application of hippocampus extract |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101780107A (en) * | 2009-01-16 | 2010-07-21 | 海南医学院 | Hippocampus extract and preparation method thereof |
| CN102008391A (en) * | 2010-09-26 | 2011-04-13 | 江苏省中医院 | Method for solidifying traditional Chinese ointment |
| CN104147237A (en) * | 2013-05-15 | 2014-11-19 | 上海海洋大学 | Anti-fatigue sea horse buccal tablet and preparation method thereof |
| KR101627837B1 (en) * | 2015-12-30 | 2016-06-07 | 전성보 | Health supplement food comprising horse bone extracts as main ingredients and sea horse and manufacturing method for the same |
-
2019
- 2019-09-17 CN CN201910873196.8A patent/CN110521965A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101780107A (en) * | 2009-01-16 | 2010-07-21 | 海南医学院 | Hippocampus extract and preparation method thereof |
| CN102008391A (en) * | 2010-09-26 | 2011-04-13 | 江苏省中医院 | Method for solidifying traditional Chinese ointment |
| CN104147237A (en) * | 2013-05-15 | 2014-11-19 | 上海海洋大学 | Anti-fatigue sea horse buccal tablet and preparation method thereof |
| KR101627837B1 (en) * | 2015-12-30 | 2016-06-07 | 전성보 | Health supplement food comprising horse bone extracts as main ingredients and sea horse and manufacturing method for the same |
Non-Patent Citations (5)
| Title |
|---|
| 张金鼎: "《海洋药物与效方》", 31 August 1998, 中医古籍出版社 * |
| 曾宪策等: "《临床常用中药400种》", 30 June 2012, 重庆出版社 * |
| 盛琳等: "海马制剂处理方法的研究", 《现代中药研究与实践》 * |
| 赵中振: "《百药图解》", 30 June 2019, 中国中医药出版社 * |
| 陈骏骐 等: "《中药药剂学》", 31 August 1989, 山东科学技术出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112369612A (en) * | 2020-10-12 | 2021-02-19 | 郑州轻工业大学 | Preparation method of donkey skin collagen peptide edible gum |
| CN114209718A (en) * | 2021-12-10 | 2022-03-22 | 海南医学院 | Preparation method and application of hippocampus extract |
| CN114209718B (en) * | 2021-12-10 | 2023-06-23 | 海南医学院 | Preparation method and application of Hippocampus extract |
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