CN101780107A - Hippocampus extract and preparation method thereof - Google Patents
Hippocampus extract and preparation method thereof Download PDFInfo
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- CN101780107A CN101780107A CN200910005010A CN200910005010A CN101780107A CN 101780107 A CN101780107 A CN 101780107A CN 200910005010 A CN200910005010 A CN 200910005010A CN 200910005010 A CN200910005010 A CN 200910005010A CN 101780107 A CN101780107 A CN 101780107A
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Abstract
The invention discloses a hippocampus extract and a preparation method thereof, belonging to the field of traditional Chinese medicines. The method comprises the following steps: coarsely smashing a hippocampus medicinal material, then carrying out enzyme hydrolysis on remaining medical slag after carrying out water-heating extraction and ethanol reflux-extraction, and finally combining hydroalcoholic extract and hydrolysis liquid to obtain the hippocampus extract. The extract can be prepared into common oral administrations such as pills, pulvis, granular formulation, oral liquid and the like. The invention has the functions of deferring senility and strengthening immunity.
Description
Technical field
The present invention relates to a kind of extraction of animal drugs, be specially Hippocampus extract and preparation method, this extract is used for slow down aging, enhance immunity etc.Belong to the field of Chinese medicines.
Background technology
Along with the raising of The development in society and economy and health care level, there has been tangible prolongation in the life-span of world population, and aged tendency of population has become worldwide trend, and the population ages of China also begins to enter veteran form.Old and feeble and defying age is the focus of world today's gerontology research, explores the effective ways and the medicine of slow down aging and life lengthening, comes into one's own just day by day.Rationally utilize Chinese medicine old and feeble this progressive process of reducing human significantly.The Chinese medicine Hippocampus all shows its good result aspect defying age on historical usage and modern pharmacological research.Hippocampus is China's famous and precious ocean of tradition Chinese crude drug, has warming the kidney to invigorate YANG, the effect of mass dissipating and swelling eliminating.Be used for sexual impotence, the enuresis is suffered from a deficiency of the kidney and is made Chuan ， lumps in the chest and abdomen, injury from falling down; External treatment carbuncle furuncle.Chemical constitution study shows that Hippocampus has higher nutrition and medical value and number of chemical active component.Protein content also has fatty acid and esters composition, steroid compound, phospholipid composition and multiple inorganic elements etc. up to more than 70%.Existing modern study provides foundation for the pharmacological action of Hippocampus defying age, has gonadotropic Effect as the Hippocampus ethanol extraction, Hippocampus also has antioxidation, delaying senility function, can regulate immunologic function, and exploitation is that the health-oriented products of raw material have good prospect with the Hippocampus.
Chinese invention patent publication number CN1478489, open day on March 3rd, 2004, Hippocampus is disclosed in the medicine of preparation treatment benign prostatic hyperplasia and the application in the health food, adopt Hippocampus complex enzyme hydrolysis product to make Hippocampus Capsule, the experiment proved that, Hippocampus has good curative effect to prostatoplasia diseases, can reach the therapeutic effect of positive control drug proscar, has the low advantage of toxicity simultaneously.
Chinese invention patent publication number CN1194143, on JIUYUE 30th, open day, a kind of sea horse oral liquor and preparation method thereof is disclosed, but the raw material that it is characterized in that used prescription mainly comprises the pure odor type Chinese liquor processes of preparing Chinese medicine of addings such as precious Chinese medicine winter worm, Ganoderma lucidum seu Japonicum of Hippocampus, shell of Chelonia mydas (Linnaeus), crystal sugar and an amount of edible medicine and forms that the ratio of its each parts by weight of raw materials of filling a prescription is: Hippocampus: shell of Chelonia mydas (Linnaeus): crystal sugar: Chinese medicine=8～15: 2～10: 20～30: 40～75.
Chinese invention patent publication number CN1858189 discloses a kind of Hippocampus japonicus liquor and preparation method thereof open day on November 8th, 2006, it is characterized in that being made by Xiaoqu rice wine, yellow wine, Hippocampus medicinal liquid, Chinese medicine soup, white sugar, the Mel of regulation umber.Described Hippocampus medicinal liquid is made by Hippocampus alive, Penis et testis cervi, Ligamentum cervi, Radix Panacis Quinquefolii, the Xiaoqu rice wine of regulation umber.Described Chinese medicine soup is made by Fructus Lycii, Herba Cistanches, the Radix Astragali, the Cortex Eucommiae, Radix Cyathulae, first GUIROU, Rhizoma Polygonati Odorati, Herba Epimedii, Radix Glycyrrhizae, the Xiaoqu rice wine of regulation umber.
Chinese invention patent publication number CN1347701 discloses day on May 8th, 2002, discloses a kind of preparation method of Seahorse powder capsule, and the Hippocampus of aquatic foods is removed internal organs, removes outside grey black epithelium, and pulverize at low temperature is encapsulated after the lyophilization.
Compare with foregoing invention, the present invention mainly obtains Hippocampus extract, improves the utilization rate of medical material, reduces taking dose, is convenient to absorb.The extract obtained slow down aging that is used for, enhance immunity.
Summary of the invention
The objective of the invention is to improve the utilization rate of Hippocampus medical material, a kind of Hippocampus extract is provided, another object of the present invention provides the preparation method (and purposes) of this Chinese medicine composition.
No matter the preparation that existing Hippocampus is used as medicine is folk prescription, compound recipe, mainly is encapsulated or steep in wine and be used as medicine by directly beating powder, gets the effect of its warming the kidney to invigorate YANG, dispersing swelling and dissipating binds.The present invention is primarily aimed at the particularity of the contained chemical constituent of animal drug Hippocampus, research Hippocampus extraction process, purpose is to make full use of raw medicinal material, protide and the extraction of phospholipid composition are entirely target as far as possible, under the prerequisite that guarantees drug effect, reduce taking dose, extract can be used for preparing the refined Haima preparation with high added value, helps absorption by human body.
In conjunction with the effect of Hippocampus use and pharmacological action as can be known, in the performance of Hippocampus drug effect, protein, amino acids are the effect that main water soluble ingredient plays nutrition, enhance immunity, and phospholipid is the effect that main liposoluble constituent plays defying age, increase body's immunity.In the research that the present invention did, respectively water and the ethanol extraction of Hippocampus are measured dried cream rate, total phospholipids content, total nitrogen content, find consistent with the solubility property of material, the fat-soluble height of phospholipid composition in the Hippocampus, could propose with certain density ethanol, and the protide composition has water solublity, and the water extraction yield is higher.Therefore two kinds of solvents of Hippocampus water alcohol are successively extracted to guarantee the yield of effective ingredient.And remaining Hippocampus medicinal residues, just can be used as the hydrolyzed protein substrate is hydrolyzed with enzyme and obtains hydrolysis amino acid and polypeptide, and proteolysis product good water solubility, utilization very easily is absorbed by the body, the patient that digestive functions such as old people preferably are tending towards weakening takes, and is consistent with adaptation population's demand of Hippocampus function.
Hippocampus extract of the present invention obtains by following measure:
The Hippocampus medicinal material coarse powder is broken, add 10-40 times of water gaging and decoct 1～3 time, each 0.5～3 hour, filter, retain behind the medicinal liquid concentrating under reduced pressure.Medicinal residues are with 8～20 times of amount alcohol reflux of 30%～90% 1～3 time, and each 0.5～3 hour, filter, collect filtrate, behind the decompression recycling ethanol, merge with water decoction, being evaporated to relative density is the medicinal liquid of 0.95～1.10 (60 ℃).Medicinal residues behind the water containing ethanol extraction, volatilize ethanol, add water to solid-to-liquid ratio 1: (1～6), regulate pH5.0～8.0, add 0.2%～2% animal proteolytic enzyme in 40 ℃～70 ℃ constant temperature hydrolysis 3～6 hours, in boiling water bath, be incubated the 5min enzyme denaturing again, take out the back squeezing and filter, filtrate and Hippocampus water containing ethanol extraction liquid merge, mix homogeneously, after centrifugal remove impurity, get Hippocampus extract.
Hydrolysising protease in the above-mentioned steps be in animal protease, neutral protease, papain, the bromelain one or more.
Preferred manufacturing procedure is as follows:
The Hippocampus medicinal material coarse powder is broken, add 30 times of water gagings and decocted 1 hour, filter, retain behind the medicinal liquid concentrating under reduced pressure.Medicinal residues filter with 15 times of amount alcohol reflux of 85% 1 hour, collect filtrate, behind the decompression recycling ethanol, merge with water decoction, and being evaporated to relative density is the medicinal liquid of 1.05 (60 ℃).Medicinal residues behind the water containing ethanol extraction, volatilize ethanol, added water to solid-to-liquid ratio 1: 2, regulate pH7.0, add 0.8% animal proteolytic enzyme in 50 ℃ of constant temperature hydrolysis 5 hours, again insulation 5min enzyme denaturing in boiling water bath, taking out the back squeezing filters, filtrate and Hippocampus water containing ethanol extraction liquid merge, and mix homogeneously gets Hippocampus extract.
The Hippocampus extract of gained of the present invention can adopt the conventional method of Chinese medicine preparation, adds suitable adjuvant, makes oral preparations such as oral liquid, granule, capsule, tablet, pill.
The Hippocampus extract of gained of the present invention has slow down aging, the effect of enhance immunity.
The specific embodiment
Below further set forth the beneficial effect of medicine of the present invention by testing example.
Hippocampus extract water of the present invention is carried [test example], the index evaluation of alcohol extraction and hydrolyzate.
Instrument and reagent
Instrument: FXB-500 type pulverizer (becoming pharmaceutical machine factory in the Hunan), kubotakr/702 centrifuge (day island proper Tianjin company), UV1600 type uv-spectrophotometric instrument (Beijing Rayleigh Instr Ltd.).
Reagent: digestive pharmaceutical (70% perchloric acid), Folch reagent (chloroform: methanol, 2: 1v/v), phosphorus colour developing liquid (3mol/L sulphuric acid: distilled water: 2.5% ammonium molybdate: 10% ascorbic acid, 1: 5: 1: 1, add by said sequence during the v/v/v/v preparation, used the same day), hydrolysising protease (Guangxi Pang Bo biological engineering company limited), other reagent are analyzes alcohol.
Experimental technique: take by weighing several parts of Hippocampus powder, use water boiling and extraction (to decoct 3 times, each 1 hour respectively, add 20 times of water gagings, extracting solution filters, concentrated standardize solution after merging), (refluxed 3 times, each 1 hour, and added 10 times of amount 75% alcohol refluxs, extracting solution filters 75% alcohol reflux, merge the back decompression recycling ethanol, standardize solution).According to chemical constituent and the effect of Hippocampus, choose total phospholipid composition and total protein class assay respectively as evaluation index, and investigate paste-forming rate.The mensuration of total phospholipid composition adopts molybdenum blue colorimetric method, and total protein class assay adopts Kjeldahl.The results are shown in Table 1.
Table 1 Hippocampus powder, decocting liquid, each index determining result of alcohol extract
The result shows after even if two kinds of solvents all extract, a lot of protide composition losses is arranged also.And the medicinal residues of Hippocampus after extraction can be used as a kind of good fish protein hydrolysis substrate just.Therefore, respectively with hydrolysising protease to the Hippocampus medical material, the medicinal residues of Hippocampus water extraction and ethanol extraction are hydrolyzed respectively.Method: take by weighing the medicinal residues (drying) after Hippocampus medical material coarse granule, Hippocampus powder, Hippocampus water are proposed medicinal residues (drying), Hippocampus alcohol extraction 3 times after 3 times, place respectively in the 100mL conical flask, all add a certain amount of water boil, be cooled to about 50 ℃, medicinal residues then adding distil water scatter, about 50 ℃ of insulations, hydrolysising condition is regulated pH, enzyme-added, insulation, 50 ℃ of hydrolysis temperatures are after hydrolysis a period of time, in boiling water bath, be incubated 5min, enzyme denaturing filters, and collects filtrate and measures dried cream rate and total nitrogen content.The results are shown in Table 2.
Dried cream rate of table 2 Hippocampus hydrolyzed solution and total nitrogen content measurement result
The result: no matter with which kind of substrate be hydrolyzed, dried cream rate and total nitrogen content that the hydrolyzed solution of gained is measured are all higher, and the Hippocampus hydrolyzed solution measurement result difference before and after extracting is not remarkable.In hydrolytic process, hydrolysising protease further decomposes substrate, and hydrolyzed solution has amino acid whose fragrance, and abnormal smells from the patient is easy to accept.Demonstration is hydrolyzed after Hippocampus is extracted again, and medical material is utilized as much as possible.With hydrolysis again after two the carrying of Hippocampus process water alcohol, can guarantee that protide composition and the phospholipid composition in the medical material has higher extraction ratio.
Come by the following examples Hippocampus extract of the present invention and preparation method thereof is described in further detail.
[embodiment 1]
The preparation of Hippocampus extract:
The Hippocampus medicinal material coarse powder is broken, add 30 times of water gagings and decocted 1 hour, filter, retain behind the medicinal liquid concentrating under reduced pressure.Medicinal residues filter with 14 times of amount alcohol reflux of 85% 1 hour, collect filtrate, behind the decompression recycling ethanol, merge with water decoction, and being evaporated to relative density is the medicinal liquid of 1.05 (60 ℃).Medicinal residues behind the water containing ethanol extraction, volatilize ethanol, added water to solid-to-liquid ratio 1: 2, regulate pH7.0, add 0.8% animal proteolytic enzyme in 50 ℃ of constant temperature hydrolysis 5 hours, again insulation 5min enzyme denaturing in boiling water bath, taking out the back squeezing filters, filtrate and Hippocampus water containing ethanol extraction liquid merge, and mix homogeneously gets Hippocampus extract.
The specific embodiment of the oral liquid preparation of [embodiment 2] medicine of the present invention.
The Hippocampus extract thin up of embodiment 1 gained to 1000ml (every mL contains the 0.1g Hippocampus), is added sucrose 20g, and fine straining is supplemented to 1000ml with distilled water, fill, and sterilization, promptly.
Using method well-known in the art, is raw material with the Hippocampus extract, makes Hippocampus Capsule.
The specific embodiment of the preparation tablets of [embodiment 3] medicine of the present invention.
The Hippocampus extract (being equivalent to contain Hippocampus 100g) of embodiment 1 gained is condensed into thick paste (about 1.10, the 60 ℃ of surveys of relative density), adds starch, mixing is granulated, drying, and granulate adds magnesium stearate, and tabletting promptly gets tablet.
The specific embodiment of the preparation tablets of [embodiment 4] medicine of the present invention.
The Hippocampus extract (being equivalent to contain Hippocampus 100g) of embodiment 1 gained is spray dried to powder, adds microcrystalline Cellulose, mixing is granulated, drying, and granulate adds magnesium stearate, and tabletting promptly gets tablet.
The specific embodiment of the capsule preparation of [embodiment 5] medicine of the present invention.
The Hippocampus extract (being equivalent to contain Hippocampus 100g) of embodiment 1 gained is condensed into thick paste (about 1.10, the 60 ℃ of surveys of relative density), adds starch, mixing is granulated, drying, and granulate in the hard capsule of packing into, promptly gets capsule.
The specific embodiment of the capsule preparation of [embodiment 6] medicine of the present invention.
The Hippocampus extract (being equivalent to contain Hippocampus 100g) of embodiment 1 gained is spray dried to powder, adds microcrystalline Cellulose, mixing is granulated, drying, and granulate in the hard capsule of packing into, promptly gets capsule.
The specific embodiment of the pill preparation of [embodiment 7] medicine of the present invention.
The Hippocampus extract (being equivalent to contain Hippocampus 100g) of embodiment 1 gained is condensed into thick paste (about 1.10, the 60 ℃ of surveys of relative density), adds micropowder silica gel, rubbing, pill.
Claims (6)
1. the preparation method of a Hippocampus extract is characterized in that: the Hippocampus medicinal material coarse powder is broken, and add 10-40 times of water gaging and decoct 1～3 time, each 0.5～3 hour, filter, retain behind the medicinal liquid concentrating under reduced pressure.Medicinal residues are with 8～20 times of amount alcohol reflux of 30%～90% 1～3 time, and each 0.5～3 hour, filter, collect filtrate, behind the decompression recycling ethanol, merge with water decoction, concentrating under reduced pressure is the medicinal liquid of 0.95～1.10 (60 ℃) to relative density.Medicinal residues behind the water containing ethanol extraction, volatilize ethanol, add water to solid-to-liquid ratio 1: (1～6), regulate pH5.0～8.0, add 0.2%～2% hydrolysising protease in 40 ℃～70 ℃ constant temperature hydrolysis 3～6 hours, again insulation 5min enzyme denaturing in boiling water bath, taking out the back squeezing filters, filtrate and Hippocampus water containing ethanol extraction liquid merge, and mix homogeneously gets Hippocampus extract.
2. Hippocampus extract according to claim 1 and preparation method thereof, it is characterized in that: the water in the step is carried with the alcohol extraction order and can be changed, promptly first ethanol extraction, medicinal residues reuse water extraction.
3. Hippocampus extract according to claim 1 and 2 and preparation method thereof is characterized in that: the hydrolysising protease in the step be in animal protease, neutral protease, papain, the bromelain one or more.
4. according to claim 1, the application of 2 or 3 Hippocampus extracts that prepare in preparation defying age health-oriented products.
5. according to claim 1, the application of 2 or 3 Hippocampus extracts that prepare in preparation enhance immunity health-oriented products.
6. can be directly medicinal according to the Hippocampus extract of claim 1,2 or 3 preparations, also can make dosage forms uses such as oral liquid, tablet, capsule, pill by adding pharmaceutic adjuvant.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101926826A (en) * | 2010-09-11 | 2010-12-29 | 海南壹号药业有限公司 | Method for extracting active ingredients from sea horse |
| CN103173512A (en) * | 2013-03-07 | 2013-06-26 | 安徽丰润生物技术有限公司 | Process for extracting Penis et Testis Canis polypeptide through enzymatic method, and obtained Penis et Testis Canis polypeptide extract |
| CN105995971A (en) * | 2016-05-13 | 2016-10-12 | 腾科宝迪（厦门）生物科技有限公司 | Nutritional liquid food for promoting diabetes recovery and preparation method thereof |
| CN106262940A (en) * | 2016-08-09 | 2017-01-04 | 福建农林大学 | A kind of gel embedding Hippocampus polypeptide microcapsule and preparation method thereof |
| CN108743915A (en) * | 2018-05-07 | 2018-11-06 | 广东海洋大学深圳研究院 | Purposes of the hippocampus polypeptide SP1 in the drug, health products or dietary supplement for preparing prevention and treatment alcoholic liver injury |
| CN110521965A (en) * | 2019-09-17 | 2019-12-03 | 海南医学院 | A kind of hippocampus glue and preparation method thereof |
| CN112315983A (en) * | 2020-11-09 | 2021-02-05 | 厦门市健康医疗大数据中心(厦门市医药研究所) | Hippocampus preparation and its preparing method and use |
| CN114209718A (en) * | 2021-12-10 | 2022-03-22 | 海南医学院 | Preparation method and application of hippocampus extract |
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2009
- 2009-01-16 CN CN200910005010A patent/CN101780107A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101926826A (en) * | 2010-09-11 | 2010-12-29 | 海南壹号药业有限公司 | Method for extracting active ingredients from sea horse |
| CN101926826B (en) * | 2010-09-11 | 2011-09-07 | 海南壹号药业有限公司 | Method for extracting active ingredients from sea horse |
| CN103173512A (en) * | 2013-03-07 | 2013-06-26 | 安徽丰润生物技术有限公司 | Process for extracting Penis et Testis Canis polypeptide through enzymatic method, and obtained Penis et Testis Canis polypeptide extract |
| CN105995971A (en) * | 2016-05-13 | 2016-10-12 | 腾科宝迪（厦门）生物科技有限公司 | Nutritional liquid food for promoting diabetes recovery and preparation method thereof |
| CN106262940A (en) * | 2016-08-09 | 2017-01-04 | 福建农林大学 | A kind of gel embedding Hippocampus polypeptide microcapsule and preparation method thereof |
| CN106262940B (en) * | 2016-08-09 | 2019-08-13 | 福建农林大学 | A kind of gel embedding hippocampus polypeptide microcapsule and preparation method thereof |
| CN108743915A (en) * | 2018-05-07 | 2018-11-06 | 广东海洋大学深圳研究院 | Purposes of the hippocampus polypeptide SP1 in the drug, health products or dietary supplement for preparing prevention and treatment alcoholic liver injury |
| CN108743915B (en) * | 2018-05-07 | 2023-02-10 | 广东海洋大学深圳研究院 | Application of hippocampus polypeptide SP1 in preparation of medicine for preventing alcoholic liver injury |
| CN110521965A (en) * | 2019-09-17 | 2019-12-03 | 海南医学院 | A kind of hippocampus glue and preparation method thereof |
| CN112315983A (en) * | 2020-11-09 | 2021-02-05 | 厦门市健康医疗大数据中心(厦门市医药研究所) | Hippocampus preparation and its preparing method and use |
| CN114209718A (en) * | 2021-12-10 | 2022-03-22 | 海南医学院 | Preparation method and application of hippocampus extract |
| CN114209718B (en) * | 2021-12-10 | 2023-06-23 | 海南医学院 | Preparation method and application of Hippocampus extract |
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Application publication date: 20100721 |