CN110499215A - A kind of extracting method of olibanum essential oil and its application in preparation proliferation of mammary gland drug - Google Patents
A kind of extracting method of olibanum essential oil and its application in preparation proliferation of mammary gland drug Download PDFInfo
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Abstract
The invention belongs to the extractive technique fields of medicinal material effective component, and in particular to a kind of extracting method of olibanum essential oil and its application in preparation proliferation of mammary gland drug.By the synergistic effect of steam distillation and microwave abstracting, efficiently olibanum extraction of essential oil is come out, the extracting method simple process, extraction efficiency is high, and manufactured olibanum essential oil can be used for preparing the medicament for treating the proliferation of mammary gland；Olibanum and myrrh, rheum officinale, Radix Angelicae Sinensis, radix paeoniae rubra, Southern Star, the coptis, dandelion, cactus interaction are made plaster hydrogel, can effectively treat the proliferation of mammary gland by mixing after fried, concentration with hot melt adhesive.
Description
Technical field
The invention belongs to the extractive technique fields of medicinal material effective component, and in particular to a kind of extracting method of olibanum essential oil and
Its application in preparation proliferation of mammary gland drug.
Background technique
Cyclomastopathy is the most common benign disease of women, neither tumour, is also not belonging to inflammation, from histological appearance
It sees: being mammoplasia and retrogression, it is closely related with endocrine dysfunction.This disease is apt to occur in middle-aged women, and blueness is few
Year and postmenopausal women also have generation, and 50%-70% has the different degrees of proliferation of mammary gland in current big city career woman.Cream
Gland hyperplasia disease often shows as breast pain and mammary gland touches tubercle.
Mazoitis is the common disease of women, can be divided into acute purulent mammitis, by mammary areola according to the difference of the cause of disease
Fistula, plasma cell mastitis etc., wherein most commonly acute purulent mammitis.Acute purulent mammitis often betides the food in one's mouth
In newborn phase, especially primipara's postpartum 1-2 month, therefore it is called acute nursing period or puerperium suppurative mastitis, Chinese medicine are known as
" acute mastitis ".The disease incidence of primipara's acute mastitis is up to 2%-4%, 1 times more than multipara mazoitis.Not only puerpera after the onset
My pain is abnormal, and cannot continue lactation, influences the health of baby.Due to factors such as current environment and life stress, suffer from
Mazoitis crowd is gradually expanded, and in addition to the women of the gestational period and nursing period, women in Development in Puberty is sitting in computer for a long time
Preceding white collar women, women that is older, not giving birth to child have the possibility for suffering from mazoitis, and number of patients rises year by year.
Western medical treatment mazoitis mostly uses operation and antibiotic medication, and not only pain is big by patient, costly and secondary
Effect is big.It is sticked with plaster, without pain, short treating period, curative effect is high.But drug at present on the market is largely treatment simultaneously
The drug of mazoitis, there are no have prevention to the proliferation of mammary gland and releive；It is therefore necessary to study a kind of pair of proliferation of mammary gland, mazoitis
There is the external drug of fabulous prevention and effect of releiving and is easy to use.
Modern pharmacological research olibanum has the pharmacology such as anti-inflammatory, antitumor, antiulcer, anti-asthma, anti-oxidant, antidepression living
Property, there are potential development and application values, and the main component of medicinal olibanum is volatile oil, resin and natural gum, wherein volatile oil
3-8% is accounted for, containing the substances such as octyl acetate and linalool, natural linalool has the function of calming the nerves calm.Some researches show that
Somalia's Olibanum volatile oil main component is firpene, and Gum Olibanum Ethiopia volatile oil main component is octyl acetate.Volatile oil
It is a kind of important component of Chinese medicine, property is special, readily volatilized lost, oxidation deterioration, to influence its curative effect.
The concocting method of olibanum is numerous, and being summed up substantially has: roast method, bran fry method, fry method, stir-baking with vinegar etc., different
Concocting method will affect oil yield, and studies have found that olibanum is using loss of volatile oil rate highest after roast method.
107267288 A of Chinese patent application CN discloses that a kind of the invention discloses a kind of the efficient of Olibanum volatile oil
Extracting method includes the following steps: that (1) cleans, cleans, and (2) steam distillation extracts, (3) volatile oil inclusion.The present invention utilizes
Steam distillation extracts Olibanum volatile oil, and is prevented using inclusion agent immediately to collected volatile oil progress inclusion is extracted
Volatile oil escapes during extraction operation and in transfer process.But the extraction efficiency of volatile oil is not high.
105586151 A of Chinese patent application CN discloses a kind of extracting method of Olibanum volatile oil, including following step
It is rapid: to take frankincense powder, be placed in extraction kettle, using supercritical CO2Extraction, extraction kettle extracting pressure 30-36MPa, extraction temperature
40-45 DEG C, extraction time 60-90min；CO2Flow velocity 200-300kg/hr；The pressure of separating still I is 8-12MPa, extraction temperature
40-50 DEG C, the pressure of separating still II is 4-6MPa, 35-40 DEG C of extraction temperature.But due to the limitation of its extracting method, answer it
With by certain limitation, meanwhile, extraction efficiency is unsatisfactory, cannot play the drug effect of Olibanum volatile oil well.
Summary of the invention
To overcome the above technical problem, the present invention provides a kind of extracting method of olibanum essential oil, preparation method is simply easy
It realizes, the recovery rate of effective component is higher, can effectively play it and prevent and treating the drug effect in proliferation of mammary gland drug.
In order to achieve the above object, technical solution provided by the invention is as follows:
A kind of extracting method of olibanum essential oil, comprising the following steps:
S1: olibanum is taken to crush；Modifying agent 1 is added, is placed in water, with extraction by steam distillation, filters to obtain residue and oil
Water mixed liquid；By oil mixing with water fluid dewatering, volatile oil 1 is obtained；
S2: residue is added in solvent, adds modifying agent 2, microwave abstracting, and filtering obtains microwave abstracting liquid；
S3: by microwave abstracting liquid rectifying, fraction is collected, volatile oil 2 is obtained；
S4: Mixed chaotic sequences 1 and volatile oil 2 are to get olibanum essential oil.
Preferably, in step S1, the dosage of the modifying agent 1 is the 0.5-1% of olibanum quality；
Preferably, the modifying agent 1 is the mixture of citric acid and acetic acid；
Preferably, the ratio of the citric acid and acetic acid is 1-3:1；
Preferably, in step S2, the solvent is ethyl alcohol or methanol, preferably ethyl alcohol；
Preferably, in step S2, the dosage of the solvent is 3-5 times of mass of residue；
Preferably, in step S2, the modifying agent 2 is propylene glycol or glycerine；
Preferably, in step S2, the dosage of the modifying agent 2 is the 5-20% of solvent quality；
Preferably, in step S3, the microwave abstracting carries out 1-2h at 500-800MHz；
Preferably, in step S4, in the distillation process, it is described collect fraction and be 150-160 DEG C and 210-220 DEG C evaporate
Point.
It include said extracted side in said preparation another object of the present invention is to provide a kind of preparation for treating the proliferation of mammary gland
The olibanum essential oil of method preparation.
Preferably, the preparation of the treatment proliferation of mammary gland, including following raw material:
Olibanum, myrrh, rheum officinale, Radix Angelicae Sinensis, radix paeoniae rubra, Southern Star, the coptis, dandelion, cactus.
Preferably, in parts by weight, the preparation of the treatment proliferation of mammary gland, including following raw material: 5-20 parts of olibanum,
5-25 parts of myrrh, 30-70 parts of rheum officinale, 10-40 parts of Radix Angelicae Sinensis, 20-50 parts of radix paeoniae rubra, 10-40 parts of Southern Star, 20-40 parts of the coptis, dandelion
40-70 parts, 150-220 parts of cactus；
Preferably, in parts by weight, the preparation of the treatment proliferation of mammary gland, including following raw material: olibanum 10-20
Part, 10-20 parts of myrrh, 50-70 parts of rheum officinale, 20-40 parts of Radix Angelicae Sinensis, 20-40 parts of radix paeoniae rubra, 20-40 parts of Southern Star, 25-40 parts of the coptis, Pu
Public English 50-70 parts, 160-200 parts of cactus；
Preferably, in parts by weight, the preparation of the treatment proliferation of mammary gland, including following raw material: 15 parts of olibanum, end
15 parts of medicine, 60 parts of rheum officinale, 30 parts of Radix Angelicae Sinensis, 30 parts of radix paeoniae rubra, 30 parts of Southern Star, 30 parts of the coptis, 30 parts of dandelion.
Preferably, the preparation of the treatment proliferation of mammary gland, further includes auxiliary material；
Preferably, the auxiliary material is hot melt adhesive；
Preferably, the raw material can be made into medicinal extract, and the dosage of the auxiliary material is that the medicinal extract gross mass after medicinal extract is made in raw material
2-3 times.
The purpose of the present invention is to provide the preparation methods of the preparation of the treatment proliferation of mammary gland, comprising the following steps:
(1) by rheum officinale, Radix Angelicae Sinensis, radix paeoniae rubra, Southern Star, the coptis, dandelion, myrrh, olibanum crushes respectively；
(2) olibanum essential oil is prepared according to the extracting method of above-mentioned olibanum essential oil；The myrrh of crushing is taken, modifying agent is added, sets
Yu Shuizhong, with extraction by steam distillation, filtering is dehydrated to obtain myrrh essential oil；
(3) cactus is squeezed the juice, smashed rheum officinale is added, Radix Angelicae Sinensis, radix paeoniae rubra, Southern Star, the coptis, dandelion is heated, dense
Contracting, obtains mixing medicinal material；
(4) medicinal material decocting will be mixed；It is concentrated to give medicinal extract；
(5) hot melt adhesive is taken, is heated, then medicinal extract, myrrh essential oil, olibanum essential oil are added, mixed, it is cooling, plaster is made
Hydrogel.
Preferably, in step (2), the dosage of the modifying agent is the 1-2% of myrrh quality；
Preferably, the modifying agent is the mixture of citric acid and acetic acid；
Preferably, the mass ratio of the citric acid and acetic acid is 1:1-2；
Preferably, in step (3), the temperature of the heating is 60-80 DEG C；
Preferably, in step (4), the quality of decocting water is 3-5 times for mixing quality of medicinal material；
Preferably, in step (4), decocting elder generation high fire (intense fire) carries out 5-12h to boiling, then under small fire (mild fire)
To ointment；
Preferably, in step (4), the decocting carries out receiving cream operation using drug thickener；
Preferably, in step (4), the decocting carries out under ultrasound condition；
Preferably, the condition of the ultrasound is 80-120KHz, is carried out under the conditions of 200-400W,
Preferably, in step (4), the specific operation process of the ultrasound are as follows: mixing medicinal material is added to the water, ultrasound is placed in
Under the conditions of, it is warming up to 40-70 DEG C, carries out 1-2h；Stop ultrasound, then is warming up to and boils；Continue 0.5-1h；
Preferably, in step (5), the quality of the hot melt adhesive is 2-3 times of medicinal extract gross mass；
Preferably, in step (5), the hot melt adhesive that is heated to is completely melt.
The object of the invention is also to provide olibanum essential oil made from the extracting method of the olibanum essential oil or the treatments
Application of the preparation of the proliferation of mammary gland in preparation proliferation of mammary gland drug.
Compared with the prior art, the technical advantages of the present invention are that:
(1) present invention assists extraction by steam distillation olibanum essential oil by using microwave abstracting, can sufficiently extract cream
Essential oil；Modifying agent is added during the extraction process can preferably separate essential oil from olibanum cell, play more preferable
Extraction effect；
(2) in the present invention, olibanum and myrrh, Southern Star etc. have synergistic effect, play the preferable inhibition proliferation of mammary gland, anti-inflammatory
Antineoplastic action；
(3) olibanum of the present invention and the extraction of effective component in myrrh are simple and efficient, can effectively and rheum officinale, Radix Angelicae Sinensis, radix paeoniae rubra, south
Star, the coptis, effective component interaction in dandelion better play inhibition and treat the effect of the proliferation of mammary gland；
(4) in the present invention, using hot melt adhesive as carrier, the plaster hydrogel of external application is made, can be made into plaster application, it can also
Promote to absorb by the method for massage again after being directly coated with, easy to use, Small side effects.
Specific embodiment
Embodiment 1
The preparation of the proliferation of mammary gland is treated in the present embodiment, in parts by weight, including following raw material: 15 parts of olibanum, end
15 parts of medicine, 60 parts of rheum officinale, 30 parts of Radix Angelicae Sinensis, 30 parts of radix paeoniae rubra, 30 parts of Southern Star, 30 parts of the coptis, 30 parts of dandelion.
Wherein, the extracting method of olibanum essential oil, comprising the following steps:
S1: olibanum is taken to crush；Be added olibanum quality 0.6% citric acid and acetic acid mixture (citric acid and acetic acid
Mass ratio is 2:1), it is placed in water, with extraction by steam distillation, filters to obtain residue and oil-water mixture；By oil-water mixture
Dehydration, obtains volatile oil 1；
S2: residue being added in 4 times of ethyl alcohol, adds 10% glycerine, and the microwave abstracting 1.5h at 600MHz is filtered,
Obtain microwave abstracting liquid；
S3: by microwave abstracting liquid rectifying, 150-160 DEG C and 210-220 DEG C of fraction is collected, volatile oil 2 is obtained；
S4: Mixed chaotic sequences 1 and volatile oil 2 are to get olibanum essential oil.
The preparation method of the preparation of the proliferation of mammary gland is treated in the present embodiment, comprising the following steps:
(1) by rheum officinale, Radix Angelicae Sinensis, radix paeoniae rubra, Southern Star, the coptis, dandelion, myrrh, olibanum crushes respectively；
(2) olibanum essential oil is prepared according to the extracting method of above-mentioned olibanum essential oil；The myrrh of crushing is taken, myrrh quality is added
2% citric acid and the mixture (mass ratio 1:1.5) of acetic acid, are placed in water, and with extraction by steam distillation, filter, take off
Water obtains myrrh essential oil；
(3) cactus is squeezed the juice, is added smashed rheum officinale, Radix Angelicae Sinensis, radix paeoniae rubra, Southern Star, the coptis, dandelion, at 70 DEG C plus
Heat, concentration, obtains mixing medicinal material；
(4) medicinal material will be mixed and decocting in 4 times of water is added；First high fire (intense fire) to boiling, then under small fire (mild fire) into
Row 10h obtains medicinal extract to ointment；
(5) it is taken as 2.5 times of hot melt adhesive of medicinal extract gross mass, hot melt adhesive is heated to and is completely melt, then by medicinal extract, myrrh
Essential oil, olibanum essential oil are added, and mix, cooling, and plaster hydrogel is made.
Embodiment 2
The preparation of the proliferation of mammary gland is treated in the present embodiment, in parts by weight, including following raw material: 5 parts of olibanum, myrrh
5 parts, 30 parts of rheum officinale, 40 parts of Radix Angelicae Sinensis, 50 parts of radix paeoniae rubra, 10 parts of Southern Star, 40 parts of the coptis, 40 parts of dandelion, 220 parts of cactus；
Wherein, the extracting method of olibanum essential oil, comprising the following steps:
S1: olibanum is taken to crush；0.5% citric acid of olibanum quality and mixture (citric acid and the acetic acid of acetic acid is added
Mass ratio be 1:1), be placed in water, with extraction by steam distillation, filter to obtain residue and oil-water mixture；By oil mixing with water
Fluid dewatering obtains volatile oil 1；
S2: residue being added in 3 times of ethyl alcohol or methanol, adds 5% propylene glycol or glycerine, and microwave extracts at 500MHz
1h is taken, filters, obtains microwave abstracting liquid；
S3: by microwave abstracting liquid rectifying, 150-160 DEG C and 210-220 DEG C of fraction is collected, volatile oil 2 is obtained；
S4: Mixed chaotic sequences 1 and volatile oil 2 are to get olibanum essential oil.
The preparation method of the preparation of the proliferation of mammary gland is treated in the present embodiment, comprising the following steps:
(1) by rheum officinale, Radix Angelicae Sinensis, radix paeoniae rubra, Southern Star, the coptis, dandelion, myrrh, olibanum crushes respectively；
(2) olibanum essential oil is prepared according to the extracting method of above-mentioned olibanum essential oil；The myrrh of crushing is taken, myrrh quality is added
1% citric acid and the mixture (mass ratio 1:1) of acetic acid, are placed in water, and with extraction by steam distillation, filter, dehydration
Obtain myrrh essential oil；
(3) cactus is squeezed the juice, is added smashed rheum officinale, Radix Angelicae Sinensis, radix paeoniae rubra, Southern Star, the coptis, dandelion, at 60 DEG C plus
Heat, concentration, obtains mixing medicinal material；
(4) it will mix in the water of 3 times of medicinal material addition and cream is received using drug thickener, and obtain medicinal extract；
(5) it is taken as 2 times of hot melt adhesive of medicinal extract gross mass, hot melt adhesive is heated to and is completely melt, then by medicinal extract, myrrh essence
Oil, olibanum essential oil are added, and mix, cooling, and plaster hydrogel is made.
Embodiment 3
The preparation of the proliferation of mammary gland is treated in the present embodiment, in parts by weight, including following raw material: 20 parts of olibanum, end
25 parts of medicine, 70 parts of rheum officinale, 10 parts of Radix Angelicae Sinensis, 20 parts of radix paeoniae rubra, 40 parts of Southern Star, 20 parts of the coptis, 70 parts of dandelion, 150 parts of cactus；
Wherein, the extracting method of olibanum essential oil, comprising the following steps:
S1: olibanum is taken to crush；Be added olibanum quality 1% citric acid and acetic acid mixture (citric acid and acetic acid
Mass ratio is 3:1), it is placed in water, with extraction by steam distillation, filters to obtain residue and oil-water mixture；By oil-water mixture
Dehydration, obtains volatile oil 1；
S2: residue being added in 5 times of methanol, adds 20% propylene glycol, the microwave abstracting 2h at 800MHz, and filtering obtains
Microwave abstracting liquid；
S3: by microwave abstracting liquid rectifying, 150-160 DEG C and 210-220 DEG C of fraction is collected, volatile oil 2 is obtained；
S4: Mixed chaotic sequences 1 and volatile oil 2 are to get olibanum essential oil.
The preparation method of the preparation of the proliferation of mammary gland is treated in the present embodiment, comprising the following steps:
(1) by rheum officinale, Radix Angelicae Sinensis, radix paeoniae rubra, Southern Star, the coptis, dandelion, myrrh, olibanum crushes respectively；
(2) olibanum essential oil is prepared according to the extracting method of above-mentioned olibanum essential oil；The myrrh of crushing is taken, myrrh quality is added
2% citric acid and the mixture (mass ratio 1:2) of acetic acid, are placed in water, and with extraction by steam distillation, filter, dehydration
Obtain myrrh essential oil；
(3) cactus is squeezed the juice, is added smashed rheum officinale, Radix Angelicae Sinensis, radix paeoniae rubra, Southern Star, the coptis, dandelion, at 80 DEG C plus
Heat, concentration, obtains mixing medicinal material；
(4) it will mix and be placed in 80KHz in the water of 5 times of medicinal material addition, and under the ultrasound condition of 200W, be warming up to 40 DEG C, carry out
2h；Stop ultrasound, then is warming up to and boils；Continue 1h；It is concentrated to give medicinal extract；
(5) it is taken as 3 times of hot melt adhesive of medicinal extract gross mass, hot melt adhesive is heated to and is completely melt, then by medicinal extract, myrrh essence
Oil, olibanum essential oil are added, and mix, cooling, and plaster hydrogel is made.
Embodiment 4
The preparation of the proliferation of mammary gland is treated in the present embodiment, in parts by weight, including following raw material: 10 parts of olibanum, end
20 parts of medicine, 50 parts of rheum officinale, 40 parts of Radix Angelicae Sinensis, 20 parts of radix paeoniae rubra, 20 parts of Southern Star, 40 parts of the coptis, 50 parts of dandelion, 160 parts of cactus；
Wherein, the extracting method of olibanum essential oil, with embodiment 1；
The preparation method of the preparation of the proliferation of mammary gland is treated in the present embodiment, comprising the following steps:
(1) by rheum officinale, Radix Angelicae Sinensis, radix paeoniae rubra, Southern Star, the coptis, dandelion, myrrh, olibanum crushes respectively；
(2) olibanum essential oil is prepared according to the extracting method of above-mentioned olibanum essential oil；The myrrh of crushing is taken, myrrh quality is added
2% citric acid and the mixture (mass ratio 1:2) of acetic acid, are placed in water, and with extraction by steam distillation, filter, dehydration
Obtain myrrh essential oil；
(3) cactus is squeezed the juice, is added smashed rheum officinale, Radix Angelicae Sinensis, radix paeoniae rubra, Southern Star, the coptis, dandelion, at 70 DEG C plus
Heat, concentration, obtains mixing medicinal material；
(4) it will mix and be placed in 120KHz in the water of 5 times of medicinal material addition, and under the ultrasound condition of 400W, be warming up to 70 DEG C, carry out
1h；Stop ultrasound, then is warming up to and boils；Continue 0.5h；It is concentrated to give medicinal extract；
(5) it is taken as 3 times of hot melt adhesive of medicinal extract gross mass, hot melt adhesive is heated to and is completely melt, then by medicinal extract, myrrh essence
Oil, olibanum essential oil are added, and mix, cooling, and plaster hydrogel is made.
Embodiment 5
The preparation of the proliferation of mammary gland is treated in the present embodiment, in parts by weight, including following raw material: 20 parts of olibanum, end
10 parts of medicine, 70 parts of rheum officinale, 20 parts of Radix Angelicae Sinensis, 40 parts of radix paeoniae rubra, 40 parts of Southern Star, 25 parts of the coptis, 70 parts of dandelion, 200 parts of cactus；
Wherein, the extracting method of olibanum essential oil, with embodiment 4；
The preparation method that the preparation of the proliferation of mammary gland is treated in the present embodiment, with embodiment 4.
Comparative example 1
Compared with Example 1, the component for preparing raw material is different, and total number is constant, but does not contain myrrh.
The preparation of the proliferation of mammary gland is treated in the present embodiment, in parts by weight, including following raw material: 30 parts of olibanum, greatly
It is 60 parts, 30 parts of Radix Angelicae Sinensis, 30 parts of radix paeoniae rubra, 30 parts of Southern Star, 30 parts of the coptis, 30 parts of dandelion yellow.
Wherein, the extracting method of olibanum essential oil, with embodiment 1.
The preparation method of the preparation of the proliferation of mammary gland is treated in the present embodiment, comprising the following steps:
(1) by rheum officinale, Radix Angelicae Sinensis, radix paeoniae rubra, Southern Star, the coptis, dandelion, olibanum crushes respectively；
(2) olibanum essential oil is prepared according to the extracting method of above-mentioned olibanum essential oil；
(3) cactus is squeezed the juice, is added smashed rheum officinale, Radix Angelicae Sinensis, radix paeoniae rubra, Southern Star, the coptis, dandelion, at 70 DEG C plus
Heat, concentration, obtains mixing medicinal material；
(4) medicinal material will be mixed and decocting in 4 times of water is added；First high fire (intense fire) to boiling, then under small fire (mild fire) into
Row 10h obtains medicinal extract to ointment；
(5) it is taken as 2.5 times of hot melt adhesive of medicinal extract gross mass, hot melt adhesive is heated to and is completely melt, then by medicinal extract, olibanum
Essential oil is added, and mixes, cooling, and plaster hydrogel is made.
Comparative example 2
Compared with Example 1, it prepares in raw material without containing Southern Star.
The preparation of the proliferation of mammary gland is treated in the present embodiment, in parts by weight, including following raw material: 15 parts of olibanum, end
15 parts of medicine, 60 parts of rheum officinale, 30 parts of Radix Angelicae Sinensis, 30 parts of radix paeoniae rubra, 30 parts of the coptis, 30 parts of dandelion.
Wherein, the extracting method of olibanum essential oil, with embodiment 1；
The preparation method of the preparation of the proliferation of mammary gland is treated in the present embodiment, comprising the following steps:
(1) by rheum officinale, Radix Angelicae Sinensis, radix paeoniae rubra, the coptis, dandelion, myrrh, olibanum crushes respectively；
(2) olibanum essential oil is prepared according to the extracting method of above-mentioned olibanum essential oil；The myrrh of crushing is taken, myrrh quality is added
2% citric acid and the mixture (mass ratio 1:1.5) of acetic acid, are placed in water, and with extraction by steam distillation, filter, take off
Water obtains myrrh essential oil；
(3) cactus is squeezed the juice, is added smashed rheum officinale, Radix Angelicae Sinensis, radix paeoniae rubra, the coptis, dandelion is heated at 70 DEG C, dense
Contracting, obtains mixing medicinal material；
(4) medicinal material will be mixed and decocting in 4 times of water is added；First high fire (intense fire) to boiling, then under small fire (mild fire) into
Row 10h obtains medicinal extract to ointment；
(5) it is taken as 2.5 times of hot melt adhesive of medicinal extract gross mass, hot melt adhesive is heated to and is completely melt, then by medicinal extract, myrrh
Essential oil, olibanum essential oil are added, and mix, cooling, and plaster hydrogel is made.
Comparative example 3
Compared with Example 1, the extracting method of olibanum is different.
The preparation of the proliferation of mammary gland is treated in the present embodiment, raw material composition is same as Example 1.
The extracting method of olibanum essential oil, comprising the following steps:
S1: olibanum is taken to crush；Be added olibanum quality 0.6% citric acid and acetic acid mixture (citric acid and acetic acid
Mass ratio is 2:1), it is placed in water, with extraction by steam distillation, filters to obtain residue and oil-water mixture；By oil-water mixture
Dehydration, obtains volatile oil to get olibanum essential oil.
The preparation method that the preparation of the proliferation of mammary gland is treated in the present embodiment, with embodiment 1.
Comparative example 4
Compared with Example 1, the extracting method of olibanum is different.
The preparation of the proliferation of mammary gland is treated in the present embodiment, raw material composition is same as Example 1.
Wherein, the extracting method of olibanum essential oil, comprising the following steps:
S1: olibanum is taken to crush；It is placed in water, with extraction by steam distillation, filters to obtain residue and oil-water mixture；It will be oily
Water mixed liquid dehydration, obtains volatile oil 1；
S2: residue being added in 4 times of ethyl alcohol, adds 10% glycerine, and the microwave abstracting 1.5h at 600MHz is filtered,
Obtain microwave abstracting liquid；
S3: by microwave abstracting liquid rectifying, 150-160 DEG C and 210-220 DEG C of fraction is collected, volatile oil 2 is obtained；
S4: Mixed chaotic sequences 1 and volatile oil 2 are to get olibanum essential oil.
The preparation method that the preparation of the proliferation of mammary gland is treated in the present embodiment, with embodiment 1.
Comparative example 5
Compared with Example 1, the preparation method for treating the preparation of the proliferation of mammary gland is different, does not add and changes in myrrh extraction process
Property agent.
The preparation of the proliferation of mammary gland is treated in the present embodiment, raw material composition is same as Example 1.
Wherein, the extracting method of olibanum essential oil is the same as embodiment 1；
The preparation method of the preparation of the proliferation of mammary gland is treated in the present embodiment, comprising the following steps:
(1) by rheum officinale, Radix Angelicae Sinensis, radix paeoniae rubra, Southern Star, the coptis, dandelion, myrrh, olibanum crushes respectively；
(2) olibanum essential oil is prepared according to the extracting method of above-mentioned olibanum essential oil；The myrrh of crushing is taken, with steam distillation
It extracts, filtering is dehydrated to obtain myrrh essential oil；
(3) cactus is squeezed the juice, is added smashed rheum officinale, Radix Angelicae Sinensis, radix paeoniae rubra, Southern Star, the coptis, dandelion, at 70 DEG C plus
Heat, concentration, obtains mixing medicinal material；
(4) medicinal material will be mixed and decocting in 4 times of water is added；First high fire (intense fire) to boiling, then under small fire (mild fire) into
Row 10h obtains medicinal extract to ointment；
(5) it is taken as 2.5 times of hot melt adhesive of medicinal extract gross mass, hot melt adhesive is heated to and is completely melt, then by medicinal extract, myrrh
Essential oil, olibanum essential oil are added, and mix, cooling, and plaster hydrogel is made.
Effect example
Mammary gland of mouse hyperplasia modeling: taking kunming mice 120, between 20 ± 2g of weight, is randomly divided into 12 groups, every group 10
Only, respectively embodiment 1-5 and comparative example 1-5, model group and blank control group, to mouse every in blank control group daily after thigh
0.9% sodium chloride injection 0.2ml of intramuscular injection, continuous injection 25 days, remaining 11 groups, with the benzene first of olive oil dilution (1:9)
Sour estradiol suspension,sterile 0.5mgkg-1, continuous 20d, be changed within the 21st day olive oil dilution (1:19) progesterone injection
5mg·kg-1, continuous 5d.
It is right in the plaster hydrogel made of 0.30g/kg embodiment 1-5 and comparative example 1-5 that is respectively coated for the 5th day of modeling
The physiological saline of blank control group coating equivalent.Continuous use 30 days, the 35th day, after 1h is administered in last time, eye socket took blood
(after 30min, 4000 turns of centrifugation 5min take serum), the level of E2, P in serum measured by radioimmunoassay.Result data is shown in Table 1:
E in 1 mice serum of table2With the variation of P content
Remarks: there is significant difference (P < 0.05) between different letters in same row.
Influence to mice serum E2 and P level compared with the control group, model group mice serum E2 it is horizontal it is significant increase, blood
Clear P level significantly reduces, and illustrates modeling success.Compared with model group, the plaster hydrogel of 1-5 of the embodiment of the present invention can make mouse
Blood serum E2 level significantly reduces, blood-serum P is horizontal significantly raised, and it is preferable to illustrate that plaster hydrogel of the invention has the proliferation of mammary gland
Therapeutic effect, and raw material composition and preparation method large effect is produced to its effect.
The experiment of breast tissue morphological change: cervical dislocation method puts to death each group mouse, complete breast is removed, with 10%
Formalin is fixed, conventional film-making, and HE dyeing does pathological analysis in light microscopic observation；To the lobule of mammary gland of experiment each group mouse
With in leaflet acinus and leaflet interstitial (every pathological section take 3 different the visual field) carry out three-dimensional meterological measurement, observation
Measuring index is the volume density (Vv) of lobule of mammary gland volume and interstitial, the specific surface (S) of mammary gland alveolus.Volume density is reaction observation
Object and shared ratio (%) in reference system, specific surface area are one sphere of reaction or a fascicular texture limitans and volume
The ratio between (%).It the results are shown in Table 2:
2 mammary gland of mouse leaflet morphology situation of change of table
Remarks: there is significant difference (P < 0.05) between different letters in same row.
Compared with the control group, the lobule of mammary gland volume density of model group, acinus specific surface dramatically increase；Plastid between leaflet
Density is substantially reduced, and illustrates modeling success.Compared with model group, the plaster hydrogel of 1-5 of the embodiment of the present invention makes mammary gland small
Leaf volume density, acinus specific surface are all substantially reduced, and leaflet interstitial volume density significantly increases.Illustrate plaster hydrogel pair of the invention
The proliferation of mammary gland has preferable therapeutic effect.And raw material composition and preparation method produce large effect to its effect.
Above-mentioned detailed description is illustrating for one of them possible embodiments of the present invention, the embodiment not to
The scope of the patents of the invention is limited, all equivalence enforcements or change without departing from carried out by the present invention are intended to be limited solely by the technology of the present invention
In the range of scheme.
Claims (10)
1. a kind of extracting method of olibanum essential oil, comprising the following steps:
S1: olibanum is taken to crush；Modifying agent 1 is added, is placed in water, with extraction by steam distillation, filter residue and grease are mixed
Close liquid；By oil mixing with water fluid dewatering, volatile oil 1 is obtained；
S2: residue is added in solvent, adds modifying agent 2, microwave abstracting, and filtering obtains microwave abstracting liquid；
S3: by microwave abstracting liquid rectifying, fraction is collected, volatile oil 2 is obtained；
S4: Mixed chaotic sequences 1 and volatile oil 2 are to get olibanum essential oil.
2. the extracting method of olibanum essential oil as described in claim 1, which is characterized in that in step S1, the dosage of the modifying agent 1
For the 0.5-1% of olibanum quality.
3. the extracting method of olibanum essential oil as described in claim 1, which is characterized in that the modifying agent 1 is citric acid and acetic acid
Mixture.
4. the extracting method of olibanum essential oil as claimed in claim 3, which is characterized in that the ratio of the citric acid and acetic acid be
1-3:1。
5. the extracting method of olibanum essential oil as described in claim 1, which is characterized in that in institute step S2, the solvent is ethyl alcohol
Or methanol；The dosage of the solvent is 3-5 times of mass of residue.
6. the extracting method of olibanum essential oil as described in claim 1, which is characterized in that in step S2, the modifying agent 2 is the third two
Alcohol or glycerine, the dosage of the modifying agent 2 are the 5-20% of solvent quality.
7. the extracting method of olibanum essential oil as described in claim 1, which is characterized in that in step S3, the microwave abstracting exists
1-2h is carried out under 500-800MHZ.
8. the extracting method of olibanum essential oil as described in claim 1, which is characterized in that in step S4, in the distillation process, institute
It states and collects the fraction that fraction is 150-160 DEG C and 210-220 DEG C.
9. a kind of preparation for treating the proliferation of mammary gland, which is characterized in that include any extraction of claim 1-8 in the preparation
The olibanum essential oil of method preparation.
10. being controlled as described in olibanum essential oil made from the extracting method of olibanum essential oil as described in claim 1-8 is any or claim 9
Treat application of the preparation of the proliferation of mammary gland in preparation proliferation of mammary gland drug.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110075246A (en) * | 2019-05-31 | 2019-08-02 | 颜苏洲 | It is a kind of to treat the nodulocystic Chinese materia medica preparation of the proliferation of mammary gland |
| CN112618532A (en) * | 2020-12-30 | 2021-04-09 | 颇黎芳香医药科技（上海）有限公司 | External medicine composition for improving hyperplasia of mammary glands |
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2019
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110075246A (en) * | 2019-05-31 | 2019-08-02 | 颜苏洲 | It is a kind of to treat the nodulocystic Chinese materia medica preparation of the proliferation of mammary gland |
| CN112618532A (en) * | 2020-12-30 | 2021-04-09 | 颇黎芳香医药科技（上海）有限公司 | External medicine composition for improving hyperplasia of mammary glands |
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