CN110496165A - A kind of Chinese medicine composition for treating periodontitis - Google Patents

A kind of Chinese medicine composition for treating periodontitis Download PDF

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CN110496165A
CN110496165A CN201910799005.8A CN201910799005A CN110496165A CN 110496165 A CN110496165 A CN 110496165A CN 201910799005 A CN201910799005 A CN 201910799005A CN 110496165 A CN110496165 A CN 110496165A
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chinese medicine
supercritical fluid
fluid extraction
periodontitis
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李艳冰
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Abstract

The invention proposes a kind of Chinese medicine compositions for treating periodontitis, belong to the field of Chinese medicines.It is made of following raw material medicaments in part by weight: 10-30 parts of pueraria lobata, 10-20 parts of bud of japanese pagoda tree, 20-30 parts of gypsum, 5-15 parts of honeysuckle, 2-10 parts of Radix Notoginseng, 5-15 parts of hawthorn, 10-20 parts of luffa and 10-30 parts of Radix zanthoxyli.Preparation method of the present invention is simple, and extract effective component is complete, and effect is obvious, and the traditional chinese medicine composition of the invention can rush down clearly stomach energy, removing pattogenic heat from the blood and toxic material from the body；Heat-clearing enriching yin；The effect of benefiting qi and nourishing blood, strong tooth support gum, has the effect for the treatment of both manifestation and root cause of disease to periodontitis.

Description

A kind of Chinese medicine composition for treating periodontitis
Technical field
The present invention relates to technical field of traditional Chinese medicines, and in particular to a kind of Chinese medicine composition for treating periodontitis.
Background technique
Periodontosis is that the mankind are most common, one of most multiple mouth disease, there is higher illness rate in world wide, Cardinal symptom is bleeding gums, and gum is red and swollen, pain, gum pyorrhea when serious, masticatory atonia, tooth mobility etc..According to third time National Oral Health Epidemiology survey data shows that the Adult Groups in China 80%~90% have different degrees of periodontal to ask Topic, account for adult total number of persons 2/3 with periodontal disease, it has also become the first killer of people's oral health.Currently, periodontosis It include mainly gingivitis and periodontitis two major classes, the former only occurs in gingiva tissue, and the latter is then to involve four kinds of periodontal supports The chronic infectious disease of (gum, parodontium, alveolar bone and cementum) is organized, the inflammatory for often causing Periodontal Supporting Tissue is broken Bad, main clinical manifestation is gingivitis, bleeding, periodontal bag formation, absorption of alveolar bone, phatnoma bone height reduction, tooth pine Dynamic, displacement, masticatory atonia, serious person's tooth can voluntarily fall off or lead to pulling out for tooth.
Clinically the treatment method of periodontosis specifically includes that 1. oral cavity is self-cleaning, electric toothbrush, scaling, subgingival debridement on gum； 2. operative treatment；3. western medicine: first nitre file, minocycline, fortimicin, for nitre file etc., but have obvious lack Point: systemic administration survival dose is big, the course for the treatment of is long, side effect is big etc.；And controlled release drug then must discharge drug at oral pocket bottom, it is necessary to It releases to the effective drug concentration of microorganism and the enough time that effective concentration must be maintained to reach in oral pocket, so that setting Medicine is difficult, periodontal lesion is big, limits the performance of curative effect.
Chinese medicine thinks that tooth is led by kidney, and deficiency of kidney essence blood is few, and tooth mistake supports, and causes sclerotin fistula soft, and with fire excess from yin deficiency, empty Inflammation is in gum meat on fire, and then tooth shakes long, root reveals；Or factor is physically weak weak, or because prolonged illness consumption hurts healthy tendency, insufficiency of vital energy and blood, gum is lost In nourishing, disease and evil is availed oneself of the opportunity to get in, thus gum meat atrophy, blood extravasation of blood, gingiva bleeding gingival hemorrhage, therefore at this disease.
Summary of the invention
The present invention provides a kind of Chinese medicine composition for treating periodontitis, it is intended that including pueraria lobata, bud of japanese pagoda tree, raw stone Cream, honeysuckle, Radix Notoginseng, hawthorn, luffa and Radix zanthoxyli.In above-mentioned traditional Chinese medicine ingredients, pueraria lobata, bud of japanese pagoda tree, gypsum, gold and silver Huadu Have the function of purging heat, moisturize, Radix Notoginseng, hawthorn, luffa and Radix zanthoxyli all have promoting blood circulation and removing obstruction in channels, wind-dispelling stagnation resolvation, alleviate toothache Effect.
The present invention provides a kind of Chinese medicine composition for treating periodontitis, is made of following raw material medicaments in part by weight: pueraria lobata 10- 30 parts, 10-20 parts of bud of japanese pagoda tree, 20-30 parts of gypsum, 5-15 parts of honeysuckle, 2-10 parts of Radix Notoginseng, 5-15 parts of hawthorn, luffa 10- 20 parts and Radix zanthoxyli 10-30 parts.
Improve as of the invention further, be made of following raw material medicaments in part by weight: 20 parts of pueraria lobata, 15 parts of bud of japanese pagoda tree, 25 parts of gypsum, 10 parts of honeysuckle, 6 parts of Radix Notoginseng, 10 parts of hawthorn, 15 parts of luffa and 20 parts of Radix zanthoxyli.
The present invention further protects a kind of preparation method of above-mentioned Chinese medicine composition, and the Chinese medicine composition is prepared as Chinese medicine Extracting solution, preparation the following steps are included:
S1. supercritical fluid extraction: the bulk pharmaceutical chemicals are weighed in proportion, after pulverizer crushes, are sieved with 100 mesh sieve, and are carried out First time supercritical fluid extraction；
The first time supercritical fluid extraction condition are as follows:
Extracting pressure: 7-10MPa；Extraction temperature: 30-50 DEG C；CO2Flow: 5-10L/h；Extraction time: 1-2h；Entrainment Agent: acetonitrile and ethyl acetate；
Second of supercritical fluid extraction is carried out after first time supercritical fluid extraction；
Second of supercritical fluid extraction condition are as follows:
Extracting pressure: 20-25MPa；Extraction temperature: 45-60 DEG C；CO2Flow: 5-10L/h；Extraction time: 1-3h；Entrainment Agent: water and ethyl alcohol；
Collect supercritical fluid extraction liquid twice and the Chinese medicine by supercritical fluid extraction twice；
S2. microwave assisted aqueous extraction extract: by by the Chinese medicine of supercritical fluid extraction twice impregnate in water for 24 hours after, it is micro- Wave aided filter repeats aforesaid operations and extracts again once, merging filtrate, and merges with supercritical fluid extraction liquid twice, low temperature It is evaporated under reduced pressure, obtaining density is 1.5-2g/cm3Concentrate to get Chinese medicine extract.
The present invention further protect a kind of above-mentioned Chinese medicine composition among the above drug composition in preparation treatment gingivitis, go out Application in the drug of bleeding from the gum that blood, tooth be aching and limp, masticatory atonia, loose shift, gum swell and chronic periodontitis.
The present invention further protect a kind of above-mentioned Chinese medicine composition and resveratrol, vitamin A and vitamin D it is any or It is a variety of combine treat aching and limp treatment gingivitis, bleeding, tooth in preparation, masticatory atonia, loose shift, gum swell bleeding from the gum and chronic Application in the drug of periodontitis.
The present invention further protects a kind of above-mentioned Chinese medicine composition to combine with minocycline hydrochloride and treats treatment tooth in preparation Application in the drug of bleeding from the gum that gingival inflammation, bleeding, tooth be aching and limp, masticatory atonia, loose shift, gum swell and chronic periodontitis.
As further improvement of the invention, the drug is exterior-applied formulation, and dosage form includes aerosol, gelling agent, gargles Aqua, toothpaste agent；The content of Chinese medicine composition is 10-15wt% in the exterior-applied formulation.
Improved as of the invention further, the drug is internal dosage form, dosage form include pill, capsule, mixture, Tablet, granule, powder；The content of Chinese medicine composition is 0.1-3wt% in the internal dosage form.
The present invention further protects a kind of aching and limp gingivitis, bleeding, tooth, masticatory atonia, loose shift, gum for the treatment of swollen The drug of bleeding from the gum and chronic periodontitis comprising above-mentioned Chinese medicine composition and pharmaceutically acceptable auxiliary material.
As further improvement of the invention, the pharmaceutic adjuvant includes ethyl alcohol, water, dextrin, microcrystalline cellulose, stearic acid Magnesium, coating agent, Arabic gum, powdery rubbing agent, wetting agent, surfactant, adhesive, fragrance, sweetener, gelatin, yellow alpine yarrow Glue, cellulose family, pool Lip river husky female 407, konjaku glucomannan, glycerol.
Pueraria lobata selected by the traditional chinese medicine composition of the invention is sweet in flavor, pungent, cool in nature, and master enters taste warp, brings down a fever with expelling pathogenic factors from muscles and skin, and promoting eruption is raw The effect of saliva quenches the thirst, Shengyang Zhixie.Bud of japanese pagoda tree is sweet in flavor, pungent, cool in nature, returns spleen, stomach meridian, brings down a fever with expelling pathogenic factors from muscles and skin, and promoting eruption is promoted the production of body fluid only Yearningly, Shengyang Zhixie effect.Gypsum is sweet in flavor, pungent, in property Great Cold, attaches to the lung and stomach meridians, and cures mainly clearing heat-fire, and relieving restlessness is quenched the thirst.For exogenous disease Pyreticosis, hyperpyrexia polydipsia, dyspnea and cough due to lung-heat, stomach fire Kang Sheng have a headache, toothache.Honeysuckle is sweet in flavor, cold in nature, return lung, the heart, stomach meridian, cures mainly clear Thermal detoxification, wind-heat dissipating.For carbuncle swells furunculosis, sore throat, erysipelas, toxic-heat and blood stasis, wind-heat cold, warm disease and fever.Radix Notoginseng is sweet, micro- Hardship, temperature, returns liver, stomach meridian, has dissipating stasis and stanching bleeding, detumescence ding-tong.For spitting blood, haematemesis, bleeding from five sense organs or subcutaneous tissue, hematochezia, metrorrhagia and metrostaxis, traumatic hemorrhage Effect.Hawthorn is sour, sweet, slightly warm in nature, and returns spleen, stomach, Liver Channel cure mainly food digesting stomach fortifying, scattered stasis.For meat stagnation, gastral cavilty Turgor, diarrhea dysentery abdominal pain, blood stasis closed, postpartum stasis, meat stagnation, dysentery are not well.Luffa is sweet in flavor, mild-natured, return lung, stomach, liver Through, there is dredging collateral, invigorates blood circulation, wind-dispelling effect.It is Radix zanthoxyli bitter, pungent, it is mild-natured, return liver, stomach meridian, there is promoting qi circulation and relieving pain, activating microcirculation and removing stasis medicinal, wind-dispelling The effect of dredging collateral.Have after said medicine compatibility and rushes down stomach energy, removing pattogenic heat from the blood and toxic material from the body clearly；Heat-clearing enriching yin；Benefiting qi and nourishing blood, strong tooth support the function of gum Effect, has the effect for the treatment of both manifestation and root cause of disease to periodontitis.Experiments have shown that traditional Chinese medicine composition for treating periodontitis of the invention, it can be effectively Periodontium local inflammation is eliminated, so that gums swelling pain and simultaneous phenomenon is alleviated, is disappeared, reaches strengthening vital QI to eliminate pathogenic factors, heat-clearing Toxin-Vanquishing, apocenosis detumescence, stop blooding permanent tooth, the purpose of deodorization analgesic therapy.
The invention has the following beneficial effects:
(1) it is of the present invention treatment periodontitis Chinese medicine composition, including pueraria lobata, bud of japanese pagoda tree, gypsum, honeysuckle, Radix Notoginseng, hawthorn, luffa and Radix zanthoxyli.In above-mentioned traditional Chinese medicine ingredients, pueraria lobata, bud of japanese pagoda tree, gypsum, gold and silver Huadu have purge heat, The effect moisturized, Radix Notoginseng, hawthorn, luffa and Radix zanthoxyli all have the function of promoting blood circulation and removing obstruction in channels, wind-dispelling stagnation resolvation, alleviate toothache.Its In Radix Notoginseng, can not only play the role of promoting blood circulation and stopping pain, go to eliminate bad breath, also there is anastalsis, promote bleeding and blood coagulation when Between significantly shorten, dencichine can play anastalsis be because it can make platelet aggregation, deformation, so that it is small to discharge bleeding The substances such as the plate factor III, calcium ion and ADP.Experiment shows that said components can cooperate when treating periodontitis, generates good Good drug effect can relieve pain faster, promote blood clotting, reduce oral pocket, kidney tonifying permanent tooth.Above-mentioned multiple-effect collective effect, The content for guaranteeing each component simultaneously can significantly increase drug effect, shorten the treatment cycle of periodontitis in range disclosed by the invention.
(2) Chinese medicine composition for the treatment of periodontitis of the present invention, is prepared into exterior-applied formulation, including Chinese medicinal toothpaste, gargles Saliva.Toothpaste can remove the bacterial plaque for being piled up in facing and gingival sulcus using the rubbing agent of toothpaste, clean to oral cavity, preferably Achieve the effect that treat periodontitis, matched with the effective component of Chinese medicine composition, can significantly alleviate the formation of dental calculus, dental plaque, And certain dental prophylaxis can be played the role of.And use Dentifrice treatment periodontitis stronger to the adaptability of patient, in daily life In the process of brushing teeth the treatment to periodontitis can be realized.Chinese medicine mouth wash can facilitate the use of patients with periodontitis, conveniently at any time It uses, and easy to carry.Peroral dosage form is fundamentally clearing heat and detoxicating, promoting blood circulation, apocenosis detumescence, and stop blooding permanent tooth, assists external application Dosage form reaches the therapeutic effect to periodontitis.
(3) extracting method of Chinese medicine of the invention extracts active ingredients from traditional Chinese medicinal using multiple supercritical fluid method, the Single extraction nonpolarity active constituent improves the dissolubility of nonpolar active constituent using acetonitrile compounding ethyl acetate as entrainer； Second of extraction polar active ingredients improves the dissolubility of polar active ingredients using water compounding ethyl alcohol as entrainer；By multiple Multiple supercritical fluid method extraction, by volatile oil most of in Chinese medicine, flavonoids, alkaloids, saponin(e, polysaccharide and quinones Etc. extracting substances come out；Using microwave radiation exaraction, by high-frequency microwave, transitory efficient extract Chinese medicine in it is remaining activity at Point, most of active ingredient of Chinese herbs is extracted, the drug activity of medicament is improved, meanwhile, preferably guarantee obtained Chinese medicine Extract can keep homogeneity after being prepared into various dosage forms for a long time.
(4) resveratrol can inhibit periodontal pathogen, hence it is evident that reduce amount of bacterial plaque, vitamin A can treat the tooth at initial stage Zhou Yan, vitamin D can promote the expression of antibacterial peptide with promoting bone growing, have auxiliary therapeutic action to periodontitis, help to maintain Oral health.The traditional chinese medicine composition of the invention combines resveratrol and vitamin A, D are used, and has more preferably therapeutic effect, faster It works.
(5) the traditional chinese medicine composition of the invention joint minocycline hydrochloride combination can be effectively improved patient's microcirculation, resist scorching Disease alleviates bleeding gums symptom, accelerates the reparation of gingiva tissue, improves clinical efficacy.
(6) preparation method of the present invention is simple, and extract effective component is complete, and effect is obvious, the traditional chinese medicine composition of the invention energy It is enough to rush down stomach energy, removing pattogenic heat from the blood and toxic material from the body clearly；Heat-clearing enriching yin；The effect of benefiting qi and nourishing blood, strong tooth support gum, has the effect for the treatment of both manifestation and root cause of disease to periodontitis Fruit.
Detailed description of the invention
Fig. 1 is the integral optical density value of MMP-13 in 1 each group rats with experimental periodontitis periodontium of test case of the present invention.
Specific embodiment
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the invention is clearly and completely described, Obviously, the embodiment described is the embodiment of part of representative of the invention, rather than whole embodiments, this field are general Other all embodiments obtained belong to protection of the invention to logical technical staff without making creative work Range.
Embodiment 1
Raw material forms (parts by weight): 10 parts of pueraria lobata, 10 parts of bud of japanese pagoda tree, 20 parts of gypsum, 5 parts of honeysuckle, 2 parts of Radix Notoginseng, mountain 5 parts of short, bristly hair or beard, 10 parts of luffa and 10 parts of Radix zanthoxyli.
The preparation method of Chinese medicine extract:
S1. supercritical fluid extraction: above-mentioned Chinese medicine is weighed in proportion, after pulverizer crushes, is sieved with 100 mesh sieve, and is carried out First time supercritical fluid extraction；
The first time supercritical fluid extraction condition are as follows:
Extracting pressure: 7MPa；Extraction temperature: 30 DEG C；CO2Flow: 5L/h；Extraction time: 1h；Entrainer: acetonitrile and second Acetoacetic ester, volume ratio 1:1；
Second of supercritical fluid extraction is carried out after first time supercritical fluid extraction；
Second of supercritical fluid extraction condition are as follows:
Extracting pressure: 20MPa；Extraction temperature: 45 DEG C；CO2Flow: 5L/h；Extraction time: 1h；Entrainer: water and second Alcohol, volume ratio 1:1；
S2. microwave assisted aqueous extraction extracts: will pass through the Chinese medicine of supercritical fluid extraction twice according to solid-to-liquid ratio 1:50g/mL Impregnate in water for 24 hours after, 500W microwave radiation exaraction 10min, filtering, repeat aforesaid operations extract again once, merging filtrate, and Merge with supercritical fluid extraction liquid twice, 35 DEG C of low-temperature reduced-pressure evaporations, obtaining density is 1.5g/cm3Concentrate to get in Medicament extracting solution.
Embodiment 2
Raw material form (parts by weight): 30 parts of pueraria lobata, 20 parts of bud of japanese pagoda tree, 30 parts of gypsum, 15 parts of honeysuckle, 10 parts of Radix Notoginseng, 15 parts of hawthorn, 20 parts of luffa and 30 parts of Radix zanthoxyli.
The preparation method of Chinese medicine extract:
S1. supercritical fluid extraction: above-mentioned Chinese medicine is weighed in proportion, after pulverizer crushes, is sieved with 100 mesh sieve, and is carried out First time supercritical fluid extraction；
The first time supercritical fluid extraction condition are as follows:
Extracting pressure: 10MPa；Extraction temperature: 50 DEG C；CO2Flow: 10L/h；Extraction time: 2h；Entrainer: acetonitrile and Ethyl acetate, volume ratio 1:1；
Second of supercritical fluid extraction is carried out after first time supercritical fluid extraction；
Second of supercritical fluid extraction condition are as follows:
Extracting pressure: 25MPa；Extraction temperature: 60 DEG C；CO2Flow: 10L/h；Extraction time: 3h；Entrainer: water and second Alcohol, volume ratio 1:1；
S2. microwave assisted aqueous extraction extracts: will pass through the Chinese medicine of supercritical fluid extraction twice according to solid-to-liquid ratio 1:50g/mL Impregnate in water for 24 hours after, 500W microwave radiation exaraction 10min, filtering, repeat aforesaid operations extract again once, merging filtrate, and Merge with supercritical fluid extraction liquid twice, 35 DEG C of low-temperature reduced-pressure evaporations, obtaining density is 2g/cm3Concentrate to get Chinese medicine Extracting solution.
Embodiment 3
Raw material forms (parts by weight): 20 parts of pueraria lobata, 15 parts of bud of japanese pagoda tree, 25 parts of gypsum, 10 parts of honeysuckle, 6 parts of Radix Notoginseng, mountain 10 parts of short, bristly hair or beard, 15 parts of luffa and 20 parts of Radix zanthoxyli.
The preparation method of Chinese medicine extract:
S1. supercritical fluid extraction: above-mentioned Chinese medicine is weighed in proportion, after pulverizer crushes, is sieved with 100 mesh sieve, and is carried out First time supercritical fluid extraction；
The first time supercritical fluid extraction condition are as follows:
Extracting pressure: 8MPa；Extraction temperature: 35 DEG C；CO2Flow: 6L/h；Extraction time: 1h；Entrainer: acetonitrile and second Acetoacetic ester, volume ratio 1:1；
Second of supercritical fluid extraction is carried out after first time supercritical fluid extraction；
Second of supercritical fluid extraction condition are as follows:
Extracting pressure: 21MPa；Extraction temperature: 50 DEG C；CO2Flow: 6L/h；Extraction time: 2h；Entrainer: water and second Alcohol, volume ratio 1:1；
S2. microwave assisted aqueous extraction extracts: will pass through the Chinese medicine of supercritical fluid extraction twice according to solid-to-liquid ratio 1:50g/mL Impregnate in water for 24 hours after, 500W microwave radiation exaraction 10min, filtering, repeat aforesaid operations extract again once, merging filtrate, and Merge with supercritical fluid extraction liquid twice, 35 DEG C of low-temperature reduced-pressure evaporations, obtaining density is 1.7g/cm3Concentrate to get in Medicament extracting solution.
Embodiment 4
Raw material forms (parts by weight): 20 parts of pueraria lobata, 15 parts of bud of japanese pagoda tree, 25 parts of gypsum, 10 parts of honeysuckle, 6 parts of Radix Notoginseng, mountain 10 parts of short, bristly hair or beard, 15 parts of luffa and 20 parts of Radix zanthoxyli.
The preparation method of Chinese medicine extract:
S1. supercritical fluid extraction: above-mentioned Chinese medicine is weighed in proportion, after pulverizer crushes, is sieved with 100 mesh sieve, and is carried out First time supercritical fluid extraction；
The first time supercritical fluid extraction condition are as follows:
Extracting pressure: 9MPa；Extraction temperature: 45 DEG C；CO2Flow: 8L/h；Extraction time: 2h；Entrainer: acetonitrile and second Acetoacetic ester, volume ratio 1:1；
Second of supercritical fluid extraction is carried out after first time supercritical fluid extraction；
Second of supercritical fluid extraction condition are as follows:
Extracting pressure: 24MPa；Extraction temperature: 55 DEG C；CO2Flow: 8L/h；Extraction time: 2h；Entrainer: water and second Alcohol, volume ratio 1:1；
S2. microwave assisted aqueous extraction extracts: will pass through the Chinese medicine of supercritical fluid extraction twice according to solid-to-liquid ratio 1:50g/mL Impregnate in water for 24 hours after, 500W microwave radiation exaraction 10min, filtering, repeat aforesaid operations extract again once, merging filtrate, and Merge with supercritical fluid extraction liquid twice, 35 DEG C of low-temperature reduced-pressure evaporations, obtaining density is 1.9g/cm3Concentrate to get in Medicament extracting solution.
The preparation of Chinese medicine mouth wash:
The additive amount for being 15wt% according to the content in Chinese medicine mouth wash by Chinese medical extract is added in mouthwash.It stirs To get to Chinese medicine mouth wash after mixing uniformly.
Embodiment 5
Raw material forms (parts by weight): 20 parts of pueraria lobata, 15 parts of bud of japanese pagoda tree, 25 parts of gypsum, 10 parts of honeysuckle, 6 parts of Radix Notoginseng, mountain 10 parts of short, bristly hair or beard, 15 parts of luffa and 20 parts of Radix zanthoxyli.
The preparation method of Chinese medicine extract:
S1. supercritical fluid extraction: above-mentioned Chinese medicine is weighed in proportion, after pulverizer crushes, is sieved with 100 mesh sieve, and is carried out First time supercritical fluid extraction；
The first time supercritical fluid extraction condition are as follows:
Extracting pressure: 8MPa；Extraction temperature: 40 DEG C；CO2Flow: 7L/h；Extraction time: 1.5h；Entrainer: acetonitrile and Ethyl acetate, volume ratio 1:1；
Second of supercritical fluid extraction is carried out after first time supercritical fluid extraction；
Second of supercritical fluid extraction condition are as follows:
Extracting pressure: 22MPa；Extraction temperature: 52 DEG C；CO2Flow: 7L/h；Extraction time: 2h；Entrainer: water and second Alcohol, volume ratio 1:1；
S2. microwave assisted aqueous extraction extracts: will pass through the Chinese medicine of supercritical fluid extraction twice according to solid-to-liquid ratio 1:50g/mL Impregnate in water for 24 hours after, 500W microwave radiation exaraction 10min, filtering, repeat aforesaid operations extract again once, merging filtrate, and Merge with supercritical fluid extraction liquid twice, 35 DEG C of low-temperature reduced-pressure evaporations, obtaining density is 1.8g/cm3Concentrate to get in Medicament extracting solution.
The preparation method of Chinese medicinal toothpaste:
Toothpaste matrix is added in the additive amount for being 10wt% according to the content in the Chinese medicinal toothpaste by Chinese medical extract In.After mixing evenly vacuum outgas to get arrive Chinese medicinal toothpaste.
Embodiment 6
Chinese medicine extract and vitamin A, vitamin D and resveratrol prepared by embodiment 3 is according to 2:0.01:0.01: 0.5 mass ratio mixing.
Embodiment 7
Chinese medicine extract and minocycline hydrochloride prepared by embodiment 3 is mixed according to the mass ratio of 2:0.01.
Comparative example 1
Raw material forms (parts by weight): 50 parts of pueraria lobata, 5 parts of bud of japanese pagoda tree, 5 parts of gypsum, 50 parts of honeysuckle, 2 parts of Radix Notoginseng, hawthorn 30 parts, 5 parts of luffa and 5 parts of Radix zanthoxyli.
The preparation method of Chinese medicine extract:
S1. supercritical fluid extraction: above-mentioned Chinese medicine is weighed in proportion, after pulverizer crushes, is sieved with 100 mesh sieve, and is carried out First time supercritical fluid extraction；
The first time supercritical fluid extraction condition are as follows:
Extracting pressure: 8MPa；Extraction temperature: 35 DEG C；CO2Flow: 6L/h；Extraction time: 1h；Entrainer: acetonitrile and second Acetoacetic ester, volume ratio 1:1；
Second of supercritical fluid extraction is carried out after first time supercritical fluid extraction；
Second of supercritical fluid extraction condition are as follows:
Extracting pressure: 21MPa；Extraction temperature: 50 DEG C；CO2Flow: 6L/h；Extraction time: 2h；Entrainer: water and second Alcohol, volume ratio 1:1；
S2. microwave assisted aqueous extraction extracts: will pass through the Chinese medicine of supercritical fluid extraction twice according to solid-to-liquid ratio 1:50g/mL Impregnate in water for 24 hours after, 500W microwave radiation exaraction 10min, filtering, repeat aforesaid operations extract again once, merging filtrate, and Merge with supercritical fluid extraction liquid twice, 35 DEG C of low-temperature reduced-pressure evaporations, obtaining density is 1.7g/cm3Concentrate to get in Medicament extracting solution.
Comparative example 2
Raw material forms (parts by weight): 20 parts of pueraria lobata, 15 parts of bud of japanese pagoda tree, 25 parts of gypsum, 10 parts of honeysuckle, 6 parts of Radix Notoginseng, mountain 10 parts of short, bristly hair or beard, 15 parts of luffa and 20 parts of Radix zanthoxyli.
The preparation method of Chinese medicine extract:
S1. supercritical fluid extraction: above-mentioned Chinese medicine is weighed in proportion, after pulverizer crushes, is sieved with 100 mesh sieve, and is carried out Supercritical fluid extraction；
The supercritical fluid extraction condition are as follows:
Extracting pressure: 8MPa；Extraction temperature: 35 DEG C；CO2Flow: 6L/h；Extraction time: 1h；Entrainer: acetonitrile and second Acetoacetic ester, volume ratio 1:1；
S2. microwave assisted aqueous extraction extracts: will pass through the Chinese medicine of supercritical fluid extraction twice according to solid-to-liquid ratio 1:50g/mL Impregnate in water for 24 hours after, 500W microwave radiation exaraction 10min, filtering, repeat aforesaid operations extract again once, merging filtrate, and Merge with supercritical fluid extraction liquid twice, 35 DEG C of low-temperature reduced-pressure evaporations, obtaining density is 1.7g/cm3Concentrate to get in Medicament extracting solution.
Comparative example 3
Raw material forms (parts by weight): 20 parts of pueraria lobata, 15 parts of bud of japanese pagoda tree, 25 parts of gypsum, 10 parts of honeysuckle, 6 parts of Radix Notoginseng, mountain 10 parts of short, bristly hair or beard, 15 parts of luffa and 20 parts of Radix zanthoxyli.
The preparation method of Chinese medicine extract:
S1. supercritical fluid extraction: above-mentioned Chinese medicine is weighed in proportion, after pulverizer crushes, is sieved with 100 mesh sieve, and is carried out Supercritical fluid extraction；
The supercritical fluid extraction condition are as follows:
Extracting pressure: 21MPa；Extraction temperature: 50 DEG C；CO2Flow: 6L/h；Extraction time: 2h；Entrainer: water and second Alcohol, volume ratio 1:1；
S2. microwave assisted aqueous extraction extracts: will pass through the Chinese medicine of supercritical fluid extraction twice according to solid-to-liquid ratio 1:50g/mL Impregnate in water for 24 hours after, 500W microwave radiation exaraction 10min, filtering, repeat aforesaid operations extract again once, merging filtrate, and Merge with supercritical fluid extraction liquid twice, 35 DEG C of low-temperature reduced-pressure evaporations, obtaining density is 1.7g/cm3Concentrate to get in Medicament extracting solution.
Comparative example 4
Raw material forms (parts by weight): 20 parts of pueraria lobata, 15 parts of bud of japanese pagoda tree, 25 parts of gypsum, 10 parts of honeysuckle, 6 parts of Radix Notoginseng, mountain 10 parts of short, bristly hair or beard, 15 parts of luffa and 20 parts of Radix zanthoxyli.
The preparation method of Chinese medicine extract:
Microwave assisted aqueous extraction extract: by Chinese medicine according to solid-to-liquid ratio 1:50g/mL impregnate in water for 24 hours after, 500W is microwave-assisted 10min is extracted, filtering repeats aforesaid operations and extracts again once, merging filtrate, and merges with supercritical fluid extraction liquid twice, 35 DEG C of low-temperature reduced-pressure evaporations, obtaining density is 1.7g/cm3Concentrate to get Chinese medicine extract.
Comparative example 5
Raw material forms (parts by weight): 20 parts of pueraria lobata, 15 parts of bud of japanese pagoda tree, 25 parts of gypsum, 10 parts of honeysuckle, 6 parts of Radix Notoginseng, mountain 10 parts of short, bristly hair or beard, 15 parts of luffa and 20 parts of Radix zanthoxyli.
The preparation method of Chinese medicine extract:
Supercritical fluid extraction: above-mentioned Chinese medicine is weighed in proportion, after pulverizer crushes, is sieved with 100 mesh sieve, and carries out first Secondary supercritical fluid extraction；
The first time supercritical fluid extraction condition are as follows:
Extracting pressure: 8MPa；Extraction temperature: 35 DEG C；CO2Flow: 6L/h；Extraction time: 1h；Entrainer: acetonitrile and second Acetoacetic ester, volume ratio 1:1；
Second of supercritical fluid extraction is carried out after first time supercritical fluid extraction；
Second of supercritical fluid extraction condition are as follows:
Extracting pressure: 21MPa；Extraction temperature: 50 DEG C；CO2Flow: 6L/h；Extraction time: 2h；Entrainer: water and second Alcohol, volume ratio 1:1；
Merge supercritical fluid extraction liquid twice, 35 DEG C of low-temperature reduced-pressure evaporations, obtaining density is 1.7g/cm3Concentrate, Up to Chinese medicine extract.
Comparative example 6
Vitamin A, vitamin D and resveratrol are mixed according to the mass ratio of 1:1:50, obtain mixture.
1 zoopery of test example
1.2 method
1.2.1 the foundation of experimental animal model of periodontitis
(1) modeling method: 256 healthy SD rats (220~240g), half male and half female, after 3% picric acid solution label It selects 24 rats as normal group using random digits table according to property ratio, weight layering, gives regular diet, it is normal to raise It supports；Remaining 224 are used as periodontitis modeling group, carry out periodontitis modeling.
Periodontitis modeling group bilateral first molar under general anesthesia ligatures correction silk, according to the daily 90mg/kg of every rat Wt. dosage, Amoxicillin (Amoxicillin, Shandong Shandong medicine limited liability company, the Ji for being 125mg/ by specification It rather) is mixed into drinking water and is fed with 3d, to inhibit to be unfavorable for porphyromonas gingivalis (Porphyromonas gingivalis, Pg) The endogenous bacteria of growth.1ml 1.5 × 10 is inoculated in the gingival sulcus of bilateral maxillary first molar12The Pg bacteria suspension of CFU/L, often 48h is inoculated with 1 time.It is inoculated with 3 times altogether.Periodontitis modeling group rat is fed with drinking water with 10% high sugar drinking-water feeding, normal rats. It is calculated after periodontitis modeling group rat maxillary first molar steel ligature, the modeling time amounts to 21d.
(2) general form is observed: after 8 rats of every group of execution, anatomical isolation goes out maxilla, is divided into arranged on left and right sides, will Left side alveolus bone specimen completely removes soft tissue, retains sclerous tissues, and normal group is observed under stereomicroscope and is modeled with periodontitis The alveolar bone situation of group rat.
(3) histological observation: after rat is put to death, it is rapidly separated maxilla, 10% neutral formalin is fixed for 24 hours, the upper jaw It is observed on the right side of bone for histotomy.Tissue pathologies change is the goldstandard that determining experimental animal model of periodontitis is successfully established, just Normal group, Periodontitis Model group randomly choose 4 right side alveolar bone tissue block (remaining 4 alveolar bones according to random digits table Tissue block is tested for follow-up immunization groupization), by the variation of pathology section examination Rat Right maxillary first molar periodontium, To determine whether modeling succeeds.
1.2.2 the traditional chinese medicine composition of the invention of various concentration treats Rat Experimental periodontitis:
(1) experimental group: Periodontal Tissues of Rat Periodontitis Model removes ligature after being successfully established, normal raising one week, using random 224 rats for being successfully established Periodontitis Model are divided into 14 groups according to random digits table: Periodontitis Model by block design Group, the traditional chinese medicine composition of the invention low dose group, middle dose group, high dose group, 16 normal rats remained after modeling successfully are made For Normal group, each group rat is 16.According to " pharmacological experimental methodology " equivalent dose coefficient Commutation Law, d rat= 6.3 × d people (d: dosage, unit: mg/kg).Medicament is used according to clinical administration dose lonvestion for 200g standard weight rat Amount is 0.8gkg-1·d-1, increase in view of rat weight in experimentation and new Chinese medicine pharmacodynamic study provide, formulate The dose gradient of 3 groups of Chinese medical extracts of the embodiment of the present invention, low dosage 0.8gkg-1·d-1, middle dosage 2gkg-1· d-1, high dose 4gkg-1·d-1, using oral gastric infusion mode.6 groups of the embodiment compositions provided for embodiment 6, Dosage is 2gkg-1·d-1, 7 groups of the embodiment compositions provided for embodiment 7, dosage 2gkg-1·d-1；Comparative example 1-6 It is all made of middle dosage 2gkg-1·d-1Stomach-filling.Positive controls select Ofloxacin, dosage 0.01gkg-1·d-1；Normally Control group and Periodontitis Model group are with 10mlkg-1·d-1Physiological saline stomach-filling.After 30d is administered, each group rat is put to death 8, remaining 8 rats continue to be administered according to the principle of grouping, all put to death after treating 60d.
(2) histological observation: to administration 30d and 60d each group rat maxilla on the right side of sample carry out immunohistochemical experiment, two A period each group sample is 8, links method using streptavidin peroxidase (streptavidinperosidase, SP) monitors expression of the MMP-13 in each experimental group gingival epithelium and parodontium.It will Know that the mouse small intestine sample for having MMP-13 strong positive to express as positive control, is made with phosphate buffer (PBS) instead of primary antibody For negative control.Positive criteria is that endochylema dyeing is yellow, and feminine gender expression is then without yellow coloring.
Image Acquisition is completed under Japanese Nikon Eclipse 80i optical microscopy, using Image-pro plus6.0 Software (Media Cybemetics, USA) carries out image analysis.Every animal selects a slice, on first molar gum The positive signal intensity of 1/3 parodontium is analyzed in skin and first molar furcation area area.With integrated absorbance (integratedabsorbance, IA) value carries out semiquantitative determination as parameter.Every slice measures its background gray scale, uses To eliminate the error that slice is shown in.
2. result
2.1 model successfully after in each group rat periodontium MMP-13 expression: the gingival epithelium and periodontal of normal rat The expression that MMP-13 is had no in film, after being successfully established Rat Experimental Periodontitis Model, gingival epithelium in Periodontitis Model group, Periodontal ligament fibroblasts in vitro and osteoblast endochylema MMP-13 are expressed in strong positive.
After 2.2 drug treatments in each group rat periodontium MMP-13 expression:
See Fig. 1, note in Fig. 1: two time point sample sizes of each group are 8；A: with identical administration time Periodontitis Model group Compared to p < 0.05, b: p < 0.05 compared with identical administration time Normal group.
After administration 30 days, Periodontitis Model group gingival epithelium MMP-13 is dyed in strong positive, and same parodontium is at fiber finer Born of the same parents and osteoblast MMP-13 are expressed in strong positive.By the high, medium and low dosage treatment of 3 Chinese medicine composition of the embodiment of the present invention Afterwards, gingival epithelium, periodontal ligament fibroblasts in vitro and osteoblast MMP-13 positive signal obviously weaken.Experimental group 1 and experiment Group 2 with vitamin A, D and resveratrol or with minocycline hydrochloride by being used in combination respectively, and the IA value of MMP-13 drops quickly To normal level, effect is better than positive controls.Compared with Example 3, bulk pharmaceutical chemicals are identical for comparative example 1, but match difference, in Dosage group is compared, and the IA value of MMP-13 significantly rises.Comparative example 2-5 is extracted using different methods, although the IA value of MMP-13 There is decline with Periodontitis Model group, but be apparently higher than the low, middle and high dose groups of embodiment 3, it is seen then that only with shooting flow Body method is extracted or microwave assisting method extracts Chinese medicine, cannot extract to obtain by all active constituents, thus the medicine influenced Effect.6 groups of comparative example compared with 6 groups of embodiment, are not added with Chinese medicine composition, and IA value is apparently higher than 6 groups of embodiment, it is seen that individually The effect of preferable treatment periodontitis cannot be played using vitamin A, vitamin D and resveratrol.By IA value it was found that, For each group compared with Periodontitis Model group and Normal group, difference has statistical significance, and the IA value of high dose group MMP-13 Substantially less than low dose group (p < 0.05).
After administration 60 days, Periodontitis Model group gingival epithelium and periodontal ligament fibroblasts in vitro endochylema MMP-13 are still in strong positive Dyeing, alveolar bone are serrated absorptions, and MMP-13 is in strong positive expression.The embodiment of the present invention 3, experimental group 1, experimental group 2 and sun The coloring degree and stained area of gingival epithelium and parodontium MMP-13 in the property high, medium and low dosage group of control group Chinese medicine composition It is substantially reduced compared with model control group, is in weakly positive, difference has statistical significance, and high dose group, experimental group 1 and experimental group The IA value of 2MMP-13 is substantially less than 1 group of comparative example (p < 0.05).
With the extension of the administration time, expression quantity decline of the MMP-13 in periodontium becomes with after 60 days within 30 days for administration Gesture.Show that the traditional chinese medicine composition of the invention can reduce inflammatory reaction, reduces the expression of Collagenase MMP-13, delay periodontium It destroys.
Test case 2
(1) observation index
This Contrast on effect example uses periodontal disease index for observation index:
0 degree: without oral pocket, tooth does not loosen, and tooth X piece shows that the tooth socket bone resorption of tooth is less than or equal to 1/5 root long.
1 degree: 3 millimeters of oral pocket, tooth does not loosen, and tooth X piece shows that the tooth socket bone resorption of tooth is less than or equal to 1/4 root It is long.
2 degree: 3-5 millimeters of oral pocket, tooth has the slight loosening that can hardly be identified, tooth X piece shows the alveolar bone of tooth Absorb the root long less than or equal to 1/3.
3 degree: 5-7 millimeters of oral pocket, for the horizontal Movable degree of tooth within 1mm, tooth X piece shows that the tooth socket bone resorption of tooth is small In or equal to 1/3 root long.
4 degree: 7-10 millimeters of oral pocket, tooth mobility is loosened in 1mm or more, and with vertical, and tooth X piece shows tooth Tooth socket bone resorption be greater than 1/2 root long.
5 degree: oral pocket is greater than 10 millimeters, and for tooth mobility in 1mm or more or to float, drifting tooth, tooth X piece shows tooth Alveolar bone be absorbed into the tip of a root.
It chooses 6 representative tooth positions 16,11,26,36,31,46 in oral cavity and is used as index tooth, if any missing then with phase adjacent teeth Substitution, measures the periodontal disease index of every tooth, and is added summation again divided by 6, obtains average periodontal disease index.
(2) experimental procedure
Respectively using the patients with periodontitis that 100 periodontal disease indexes are 4 degree as test group 1, test group 2, in addition with 100 teeth The patients with periodontitis that all disease indexes are 4 degree is control group.
Test group 1-3: the Chinese medicine of the treatment periodontitis successively prepared with the embodiment of the present invention 3, embodiment 6, embodiment 7 mentions Liquid is taken, 2 times a day, affected part is sprayed every time and keeps medicinal powder 15 minutes in mouth.
Test group 4-8: the Chinese medicine extract successively prepared using comparative example 1-5 of the invention is sprayed every time 2 times a day Affected part simultaneously keeps medicinal powder 15 minutes in mouth.
Test group 9: water is dissolved in using the mixture of comparative example 6 of the invention, independent density is 1.5-2g/cm3Liquid, 2 times a day, affected part is sprayed every time and keeps medicinal powder 15 minutes in mouth.
Control group: using powder made of tortoise plastron, Rhizoma Chuanxiong, rhizoma cyperi, lotus leaf, the root of Dahurain angelica, Chinese prickly ash, Cortex hibisus syriacus, halite, daily 2 times, affected part is sprayed every time and keeps medicinal powder 15 minutes in mouth.
Test group 1 can averagely be relieved pain using 2-3 days, substantially reduce within 4-7 days times of bleeding, oral pocket is significant within 30-40 days Reduce, 80-90 days firm teeth realize recovery from illness.
Test group 2-3 averagely uses can relieve pain for 1-2 days, substantially reduces within 2-4 days times of bleeding, oral pocket is aobvious within 10-20 days It writes and reduces, 30-40 days firm teeth realize recovery from illness.
Test group 4 is for relieving pain, reducing times of bleeding, oral pocket etc. without apparent curative effect.
Test group 5-8 averagely uses can relieve pain for 5-10 days, substantially reduces within 7-12 days times of bleeding, 50-60 days oral pockets It is substantially reduced, 120-130 days firm teeth realize recovery from illness.
Test group 9 is for relieving pain, reducing times of bleeding, oral pocket etc. without apparent curative effect.
Control group averagely uses relieve pain for 3-5 days, and times of bleeding substantially reduces within 10-15 days, and oral pocket significantly subtracts within 60-70 days Small, 150 days or so firm teeth realize recovery from illness.
Patient reaches the required treatment time that periodontal disease index is 0 degree: 1 average out to of test group 90 days, and test group 2 is average It is 60 days, 3 average out to of test group 50 days, test group 4 cannot make periodontal disease index be reduced to 0 degree, and 5 average out to of test group 100 days tries Test group 6 average out to 105 days, 7 average out to of test group 110 days, 8 average out to of test group 105 days, test group 9 cannot be such that periodontosis refers to Number is reduced to 0 degree, control group average out to 150 days.
In therapeutic process, control group has two people not to be resistant to drug tastes to exit, and has three people because troublesome in poeration final lost to follow-up； Test group 4 has two people because troublesome in poeration final lost to follow-up；Test group 5 has three people because troublesome in poeration final lost to follow-up, and test group 7 has a people Because troublesome in poeration final lost to follow-up, test group 9 has two people not to be resistant to drug tastes to exit, and the no one of test group 1-3,6,8 exits, And it indicates to continue to use.
(3) conclusion
The Chinese medicine composition for the treatment of periodontitis of the invention, curative effect are more preferable compared to the drug of existing treatment periodontitis, Treatment process is accelerated, it can be achieved that patients with periodontitis is comparatively fast fully recovered, medication is convenient, is easy to be accepted by patients.
Compared with prior art, the Chinese medicine composition for the treatment of periodontitis of the present invention, including pueraria lobata, bud of japanese pagoda tree, life Gypsum, honeysuckle, Radix Notoginseng, hawthorn, luffa and Radix zanthoxyli.In above-mentioned traditional Chinese medicine ingredients, pueraria lobata, bud of japanese pagoda tree, gypsum, honeysuckle All have the function of purging heat, moisturize, Radix Notoginseng, hawthorn, luffa and Radix zanthoxyli all have promoting blood circulation and removing obstruction in channels, wind-dispelling stagnation resolvation, alleviate tooth The effect of pain.Radix Notoginseng therein can not only play the role of promoting blood circulation and stopping pain, go to eliminate bad breath, and also have anastalsis, promote bleeding Significantly shorten with the time of blood coagulation, dencichine can play anastalsis be because it can make platelet aggregation, deformation, thus Release the substances such as platelet factor III, calcium ion and ADP.Experiment shows that said components can be mutually when treating periodontitis Cooperation, generates good drug effect, can relieve pain faster, promote blood clotting, reduces oral pocket, kidney tonifying permanent tooth.Above-mentioned multiple effect Collective effect is answered, while the content for guaranteeing each component can significantly increase drug effect in range disclosed by the invention, shorten periodontitis Treatment cycle.
The Chinese medicine composition for the treatment of periodontitis of the present invention, is prepared into exterior-applied formulation, including Chinese medicinal toothpaste, gargles Water.Toothpaste can remove the bacterial plaque for being piled up in facing and gingival sulcus using the rubbing agent of toothpaste, clean to oral cavity, preferably reach To the effect for the treatment of periodontitis, is matched with the effective component of Chinese medicine composition, can significantly alleviate the formation of dental calculus, dental plaque, and It can play the role of certain dental prophylaxis.And use Dentifrice treatment periodontitis stronger to the adaptability of patient, in daily life The process of brushing teeth the treatment to periodontitis can be realized.Chinese medicine mouth wash can facilitate the use of patients with periodontitis, convenient to make at any time With, and it is easy to carry.Peroral dosage form is fundamentally clearing heat and detoxicating, promoting blood circulation, apocenosis detumescence, and stop blooding permanent tooth, assists external preparation Type reaches the therapeutic effect to periodontitis.
The extracting method of Chinese medicine of the invention, using multiple supercritical fluid method extract active ingredients from traditional Chinese medicinal, first Secondary extracting apolar active constituent improves the dissolubility of nonpolar active constituent using acetonitrile compounding ethyl acetate as entrainer；The Reextraction polar active ingredients improve the dissolubility of polar active ingredients using water compounding ethyl alcohol as entrainer；By repeatedly more Secondary supercritical fluid method extraction, by volatile oil most of in Chinese medicine, flavonoids, alkaloids, saponin(e, polysaccharide and quinones etc. Extracting substances come out；Using microwave radiation exaraction, by high-frequency microwave, transitory efficient extract Chinese medicine in it is remaining activity at Point, most of active ingredient of Chinese herbs is extracted, the drug activity of medicament is improved, meanwhile, preferably guarantee obtained Chinese medicine Extract can keep homogeneity after being prepared into various dosage forms for a long time.
Resveratrol can inhibit periodontal pathogen, hence it is evident that reduce amount of bacterial plaque, vitamin A can treat the periodontal at initial stage Inflammation, vitamin D can promote the expression of antibacterial peptide with promoting bone growing, have auxiliary therapeutic action to periodontitis, help to maintain mouth Chamber health.The traditional chinese medicine composition of the invention combines resveratrol and vitamin A, D are used, and has more preferably therapeutic effect, faster rises Effect.
The traditional chinese medicine composition of the invention joint minocycline hydrochloride combination can be effectively improved patient's microcirculation, resist inflammation, Alleviate bleeding gums symptom, accelerate the reparation of gingiva tissue, improves clinical efficacy.
Preparation method of the present invention is simple, and extract effective component is complete, and effect is obvious, and the traditional chinese medicine composition of the invention can be clear Rush down stomach energy, removing pattogenic heat from the blood and toxic material from the body；Heat-clearing enriching yin；The effect of benefiting qi and nourishing blood, strong tooth support gum, has the effect for the treatment of both manifestation and root cause of disease to periodontitis.
Those skilled in the art is not under conditions of departing from the spirit and scope of the present invention that claims determine, also Various modifications can be carried out to the above content.Therefore the scope of the present invention is not limited in above explanation, but by The range of claims determines.

Claims (10)

1. a kind of Chinese medicine composition for treating periodontitis, which is characterized in that be made of following raw material medicaments in part by weight: pueraria lobata 10- 30 parts, 10-20 parts of bud of japanese pagoda tree, 20-30 parts of gypsum, 5-15 parts of honeysuckle, 2-10 parts of Radix Notoginseng, 5-15 parts of hawthorn, luffa 10- 20 parts and Radix zanthoxyli 10-30 parts.
2. a kind of Chinese medicine composition for treating periodontitis according to claim 1, which is characterized in that by following bulk pharmaceutical chemicals by weight Measure part composition: 20 parts of pueraria lobata, 15 parts of bud of japanese pagoda tree, 25 parts of gypsum, 10 parts of honeysuckle, 6 parts of Radix Notoginseng, 10 parts of hawthorn, luffa 15 Part and 20 parts of Radix zanthoxyli.
3. according to claim 1 or the preparation method of any Chinese medicine composition described in 2, which is characterized in that the Chinese medicine group Close object and be prepared as Chinese medicine extract, preparation the following steps are included:
S1. supercritical fluid extraction: the bulk pharmaceutical chemicals are weighed in proportion, after pulverizer crushes, are sieved with 100 mesh sieve, and carry out first Secondary supercritical fluid extraction；
The first time supercritical fluid extraction condition are as follows:
Extracting pressure: 7-10MPa；Extraction temperature: 30-50 DEG C；CO2Flow: 5-10L/h；Extraction time: 1-2h；Entrainer: second Nitrile and ethyl acetate；
Second of supercritical fluid extraction is carried out after first time supercritical fluid extraction；
Second of supercritical fluid extraction condition are as follows:
Extracting pressure: 20-25MPa；Extraction temperature: 45-60 DEG C；CO2Flow: 5-10L/h；Extraction time: 1-3h；Entrainer: water And ethyl alcohol；
Collect supercritical fluid extraction liquid twice and the Chinese medicine by supercritical fluid extraction twice；
S2. microwave assisted aqueous extraction extract: by by the Chinese medicine of supercritical fluid extraction twice impregnate in water for 24 hours after, microwave is auxiliary Filtering is helped, aforesaid operations is repeated and extracts again once, merging filtrate, and merge with supercritical fluid extraction liquid twice, low-temperature reduced-pressure Evaporation, obtaining density is 1.5-2g/cm3Concentrate to get Chinese medicine extract.
4. any Chinese medicine composition as stated in claim 1 or 2 is in preparation treatment gingivitis, bleeding, tooth are aching and limp, nozzle Chew the application in the drug of the swollen bleeding from the gum of inability, loose shift, gum and chronic periodontitis.
5. any Chinese medicine composition as stated in claim 1 or 2 in resveratrol, vitamin A and vitamin D It is any one or more combine treat aching and limp treatment gingivitis, bleeding, tooth in preparation, masticatory atonia, loose shift, gum swell tooth Application in the drug of nosebleed and chronic periodontitis.
6. any Chinese medicine composition as stated in claim 1 or 2 is combined with minocycline hydrochloride treats treatment tooth in preparation Application in the drug of bleeding from the gum that gingival inflammation, bleeding, tooth be aching and limp, masticatory atonia, loose shift, gum swell and chronic periodontitis.
7. the application according to any one of claim 4-6, which is characterized in that the drug is exterior-applied formulation, dosage form packet Include aerosol, gelling agent, aqua of gargling, toothpaste agent；The content of Chinese medicine composition is 10-15wt% in the exterior-applied formulation.
8. the application according to any one of claim 4-6, which is characterized in that the drug is internal dosage form, dosage form packet Include pill, capsule, mixture, tablet, granule, powder；The content of Chinese medicine composition is 0.1- in the internal dosage form 3wt%.
9. it is a kind of treat aching and limp gingivitis, bleeding, tooth, masticatory atonia, loose shift, gum swell bleeding from the gum and chronic periodontitis Drug comprising any Chinese medicine composition described in claims 1 or 2 and pharmaceutically acceptable auxiliary material.
10. drug according to claim 9, which is characterized in that the pharmaceutically acceptable auxiliary material be selected from ethyl alcohol, water, Dextrin, microcrystalline cellulose, magnesium stearate, coating agent, Arabic gum, powdery rubbing agent, wetting agent, surfactant, adhesive, Fragrance, sweetener, gelatin, bassora gum, cellulose family, pool Lip river husky female 407, konjaku glucomannan, glycerol.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113679767A (en) * 2021-10-13 2021-11-23 李艳冰 Traditional Chinese medicine composition for treating periodontitis

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103222947A (en) * 2013-05-08 2013-07-31 苏敏华 Traditional Chinese medicinal composition for treating periodontitis
CN103784856A (en) * 2012-10-31 2014-05-14 成都九芝堂金鼎药业有限公司 Traditional Chinese medicine composition for treating periodontitis, and preparation method and application thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103784856A (en) * 2012-10-31 2014-05-14 成都九芝堂金鼎药业有限公司 Traditional Chinese medicine composition for treating periodontitis, and preparation method and application thereof
CN103222947A (en) * 2013-05-08 2013-07-31 苏敏华 Traditional Chinese medicinal composition for treating periodontitis

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113679767A (en) * 2021-10-13 2021-11-23 李艳冰 Traditional Chinese medicine composition for treating periodontitis

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