CN110488002B - Immunochromatography device convenient to add liquid - Google Patents
Immunochromatography device convenient to add liquid Download PDFInfo
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- CN110488002B CN110488002B CN201910815215.1A CN201910815215A CN110488002B CN 110488002 B CN110488002 B CN 110488002B CN 201910815215 A CN201910815215 A CN 201910815215A CN 110488002 B CN110488002 B CN 110488002B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
Abstract
The invention relates to the technical field of biological detection devices, in particular to an immunochromatography device convenient for adding liquid, which comprises a base, wherein a liquid mixing mechanism is arranged on the base and is used for automatically mixing and preparing a color development liquid for immunochromatography detection; the reaction mechanism is used for obtaining the color developing solution mixed by the solution mixing mechanism and simultaneously enabling the color developing solution to automatically react with the test paper added with the sample to be detected in a sealing manner; the detection mechanism is used for automatically detecting the reaction result, and analyzing and storing the reaction result; and the control system is used for controlling the operations of liquid mixing, reaction and detection in the immunochromatography detection to be sequentially carried out. According to the invention, the immunochromatography device is used for automatically reacting and detecting the sample, and the developing solution can be obtained by automatically mixing the solution, so that the problems of easy error and low detection efficiency of manual detection in the prior art are solved, the detection efficiency of immunochromatography is improved, and the detection accuracy is improved.
Description
Technical Field
The invention relates to the technical field of biological detection devices, in particular to an immunochromatography device convenient for adding liquid.
Background
Immunochromatography (immunochromatography) is a rapid diagnostic technique based on the principle that specific antibodies are first immobilized in a zone on a solid support on which capillary action is caused. When the dried solid support is immersed in a sample at one end, the sample will move forward along the membrane due to capillary action, and when moving to the area where the antibody is fixed, the corresponding antigen in the sample will specifically bind to the antibody, and if the area is stained with immune colloidal gold or immune enzyme, the area will show a certain color, thereby realizing specific immunodiagnosis.
Most of diagnosis devices in the market are semi-automatic devices at present, and auxiliary operation needs to be carried out manually in the diagnosis process, for example, in the color developing solution preparation stage, some color developing solutions need to be prepared currently, different liquids need to be mixed manually to obtain the color developing solutions in order to ensure the detection accuracy, the base number of detection samples is large during actual detection, the quantity of the color developing solutions is large, the color developing solutions are prepared only by manual on-site, and the problems of inaccurate preparation proportion, uneven mixing and color developing solution waste easily occur in manual operation; meanwhile, because the cardinal number of the detection sample is large, the test paper dropping color developing solution of the dropping detection sample is required to be continuously dropped during chromatography, the dropping is inaccurate and forgets the dropping, and other problems exist during the manual dropping color developing solution, the detection result is directly influenced by the various problems, and in order to make the detection result more accurate, a detection device capable of automatically mixing, reacting and detecting is urgently needed to be designed.
Disclosure of Invention
In order to solve the problems, the invention provides an immunochromatographic device convenient for adding liquid, solves the problem that immunochromatographic detection in the prior art cannot automatically mix liquid, react and detect, and improves the accuracy of a detection result.
In order to achieve the purpose, the invention adopts the technical scheme that: an immunochromatography device convenient for adding liquid comprises a base, wherein a liquid mixing mechanism, a reaction mechanism, a detection mechanism and a control system are arranged on the base,
the liquid mixing mechanism is used for automatically mixing and preparing color developing liquid for immunochromatography detection, and comprises a liquid suction cup, two mixing cavities are arranged in the liquid suction cup, liquid suction needles which can be inserted into the mixing cavities and are mutually communicated are arranged in each mixing cavity, and a mixing driving piece for driving the liquids in the two mixing cavities to be instantly exchanged and mixed is connected to each liquid suction needle;
the reaction mechanism is used for obtaining the color developing solution mixed by the solution mixing mechanism and simultaneously enabling the color developing solution to automatically react with the test paper added with the sample to be detected in a sealing manner;
the detection mechanism is used for automatically detecting the reaction result, and analyzing and storing the reaction result;
and the control system is used for controlling the operations of liquid mixing, reaction and detection in the immunochromatography detection to be sequentially carried out.
The principle of the invention is as follows: the liquid mixing mechanism is used for mixing liquid to prepare color developing liquid, A, B liquid and two kinds of liquid for preparing the color developing liquid are respectively poured into two mixing cavities of a liquid suction cup at the initial stage of liquid mixing, the two mixing cavities are communicated by a liquid suction needle, a liquid suction needle is driven by a mixing driving piece to suck liquid, such as liquid A, in one mixing cavity, the sucked liquid is discharged into the other mixing cavity through the liquid suction needle, namely the mixing cavity for containing liquid B is filled, the two kinds of liquid are preliminarily mixed, all the preliminarily mixed liquid is sucked into the mixing cavity filled with the liquid A by the mixing driving piece (at the moment, the liquid A in the mixing cavity for originally transferring the liquid A is completely sucked into the mixing cavity filled with the liquid B), so that the liquid A, B is mixed again, the liquid suction needle is driven by the mixing driving piece to suck and spit repeatedly, the liquid A, B liquid is continuously and repeatedly mixed between the two mixing cavities, and the liquid A, the liquid A and the liquid B are finally mixed, Uniformly mixing the solution B to form a color development solution for immunochromatography detection; after the color developing solution is prepared, the control system controls the reaction mechanism to extract the color developing solution from the solution mixing mechanism, the color developing solution and the test paper added with the sample to be detected are subjected to automatic sealing reaction, the chromatography time is accurately controlled through the control system, then the control system controls the detection mechanism to automatically detect the reaction result, and the detection result is analyzed and recorded.
The scheme has the advantages that:
1. the automation degree and the diagnosis efficiency are high; compared with the manual diagnosis and detection in the prior art, the manual operation is low in efficiency, in the application, the configuration from the color developing solution to the immunochromatography detection and the analysis and the storage of the detection result are automatically completed by depending on the cotton-padded clothes chromatography device, the automation degree is high during the detection, and the detection efficiency is high.
2. The detection result is more accurate: compared with manual immunochromatography detection in the prior art, the immunochromatography detection method has the problems of uneven liquid mixing, liquid waste, inaccurate detection time and the like during manual operation, and reduces the accuracy of detection results. In the application, the stages of color development liquid configuration, detection reaction and detection result analysis are automatically completed in the immunochromatography device, so that the condition of manual error operation is avoided; meanwhile, the chromatography time is strictly controlled by using the control system, so that the chromatography reaction time is more in accordance with the standard, the detection result is more accurate, and the detection result in the application is more accurate.
3. When the color developing solution is prepared, the color developing solution can be prepared at present and can be uniformly mixed: when the color developing solution is prepared, the color developing solution needs to be prepared at present, A, B solution needs to be uniformly mixed during the preparation, if the preparation is carried out manually, a long time is needed for dropwise adding and mixing A, B solution back and forth, and when a large number of samples are detected, the color developing solution cannot be prepared manually and rapidly. In this application, at first pour A, B liquid into respectively in two hybrid chambers of imbibition cup, the configuration proportion of A, B liquid can be regulated and control accurately, utilizes hybrid drive spare to make a round trip to inhale A, B liquid in two hybrid chambers of imbibition cup simultaneously and tell to replace artifical mixing to A, B liquid, the misce bene just can satisfy the immunochromatography and detect the needs.
Further, be equipped with first drive unit on the base, first drive unit includes that the board is placed to the imbibition, first imbibition drive spare, second imbibition driving piece, and the imbibition cup can be dismantled and connect on the board is placed to the imbibition, and first imbibition drive spare is used for placing the board horizontal drive with the imbibition, and second imbibition driving piece is used for driving the imbibition needle to insert and withdraw from the hybrid chamber.
When the color developing liquid is required to be prepared, A, B can be mixed after the liquid sucking needles are inserted by the second liquid sucking driving piece; and when adding A, B liquid, can utilize the second imbibition driving piece to withdraw from the mixing chamber with the imbibition needle, then utilize first imbibition driving piece to place the board drive with the imbibition and keep away from the imbibition needle, the imbibition needle is kept away from to the imbibition cup this moment, makes the imbibition needle not influence A, B liquid's addition to can conveniently add A, B liquid in the mixing chamber in the imbibition cup.
Further, the reaction mechanism comprises a reagent clamping groove connected to the base in a sliding manner, a reagent card box is detachably connected in the reagent clamping groove, a test strip is arranged in the reagent card box, and a liquid feeding hole and a waste liquid hole are formed in the reagent card box; the base is connected with a liquid feeding needle which can be opposite to the liquid feeding hole in a sliding way, and the liquid feeding needle is communicated with the liquid sucking needle.
The A, B liquid is uniformly mixed by the mixed driving piece to form the color developing liquid, then the color developing liquid is discharged to the liquid adding needle by the mixed driving piece, the color developing liquid is dripped into the liquid adding hole by the liquid adding needle, and the color developing liquid reacts with the sample to be detected on the test paper after the color developing liquid is respectively added to the test paper by the liquid adding hole because the sample to be detected is already added on the test paper in the reagent card box, thereby realizing the detection of the immunochromatography.
Furthermore, a waste liquid needle capable of facing the waste liquid hole is connected to the base in a sliding mode, a waste liquid pump communicated with the waste liquid needle is fixedly connected to the base, and the waste liquid pump is connected with the control system.
Utilize opening or closing of control system control waste liquid pump, when the waste liquid pump was opened, the waste liquid pump ordered about the waste liquid needle and siphoned away the waste liquid through the reaction in the reagent cassette from the waste liquid hole to the reaction time of control sample and color development liquid that awaits measuring makes the testing result more accurate, utilizes the waste liquid needle to siphone away the waste liquid simultaneously, avoids the waste liquid to cause the influence to follow-up detection mechanism's detection, further promotes the accuracy of testing result.
Further, reaction mechanism still includes first reaction driving piece and second reaction driving piece of fixed connection on the base, and first reaction driving piece is used for ordering about the reagent draw-in groove and slides on the base, and the board is placed in fixedly connected with reaction on the second reaction driving piece, and liquid feeding needle and waste liquid needle all can be dismantled and connect on the board is placed in the reaction.
The reagent clamping groove is driven to slide on the base by the first reaction driving part, when color developing liquid needs to be dripped into the reagent clamping groove, the reagent clamping groove is driven to the position below the liquid adding needle, and then the liquid adding needle and the waste liquid needle are pushed to the positions corresponding to the reagent clamping groove by the second reaction driving part, so that liquid adding and waste liquid absorption are realized; and after the test was accomplished, the second reaction driving piece promoted to add liquid needle and waste liquid needle and kept away from reagent card box, then utilizes first reaction driving piece to promote the reagent card box to the position of keeping away from with liquid needle and waste liquid needle to conveniently change the reagent card box in the reagent card box, so that carry out next sample detection fast.
Further, the reaction is placed and is opened on the board and is had the centre gripping through-hole that is used for placing liquid feeding needle and waste liquid needle, and the reaction is placed and is opened on the board and have the activity groove that is the L type and run through the reaction and place the board, and the one end of activity groove is placed the lateral wall of board with the reaction and is link up, the other end and centre gripping through-hole link up.
After the reaction placing plate is provided with the clamping through hole for clamping the liquid adding needle and the waste liquid needle, the reaction placing plate is provided with the L-shaped movable groove which penetrates through the reaction placing plate, one end of the movable groove is communicated with the side edge of the reaction placing plate, the other end of the movable groove is communicated with the clamping through hole, when the clamping through hole is manually extruded or pulled, the size of the clamping through hole can be changed, after the action of external force disappears, the clamping through hole recovers to the original size under the action of the self restoring force of the reaction placing plate, after the movable groove is arranged, when the liquid adding needle and the waste liquid needle need to be fixed, the aperture of the clamping through hole is increased manually, then the liquid adding needle or the waste liquid needle is placed, then the external force disappears, the liquid adding needle or the waste liquid needle can be clamped when the clamping through hole recovers to the original size, compared with the fixation of the liquid adding needle or the waste liquid needle by external parts such as screws and the like, it is very convenient to add the fixed of liquid needle and waste liquid needle in this scheme, the aperture change that also enables external force simultaneously makes the centre gripping through-hole makes the reaction place the board temporarily disappear to the clamping action of liquid feeding needle or waste liquid needle, thereby the convenience is adjusted the position of liquid feeding needle or waste liquid needle, after the position of liquid feeding needle or waste liquid needle has been adjusted, the external force of release to the centre gripping through-hole makes its quick recovery to original size, make liquid feeding needle or waste liquid needle fixed again fast, it is very convenient to add liquid needle and waste liquid needle position control.
Furthermore, the second driving piece comprises a second reaction motor fixedly connected to the base, a lead screw is fixedly connected to the second reaction motor, a guide shaft is fixedly connected to the base and arranged in parallel with the lead screw, and a threaded hole matched with the lead screw in a threaded mode and a guide hole matched with the guide shaft are formed in the reaction placing plate.
Compare in using belt or gear to carry out the transmission, in this scheme, utilize the electronic lead screw of motor to rotate, because the guide effect of guiding axle, the lead screw rotates and drives the reaction and places the board along the endwise slip of lead screw, thereby the removal of liquid needle and waste liquid needle on the board is placed in the drive reaction, utilize screw drive, can effectively keep the precision of feeding of liquid needle or waste liquid needle, thereby guarantee to add the liquid needle, the waste liquid needle can with the accurate cooperation of reagent card box, realize accurate liquid feeding and absorption waste liquid.
Furthermore, the detection mechanism comprises a camera, and an image analysis system and a storage system are connected outside the camera.
After carrying out chromatography reaction on the test paper with color development liquid dropwise add in the reagent card box, utilize second reaction actuating mechanism to order about liquid feeding needle and waste liquid needle and keep away from the reagent card box, utilize first reaction driving piece to push away reagent card box and reagent card box to the camera below together simultaneously, utilize the camera to shoot the test paper after the reaction in the reagent card box, will shoot the result and send to image analysis system simultaneously, utilize image analysis system to judge the testing result, and the automatic storage to storage system of result that obtains picture and the analysis of will shooing.
Further, a light source for supplementing light to the camera is fixedly connected to the base.
Light is supplemented to the camera by the light source, so that the pictures shot by the camera are clearer, and the accuracy of the detection result is improved.
Further, fixedly connected with diffusion sheet on the base, diffusion sheet is located between light source and the camera, and the diffusion sheet can surround camera and reagent card box.
The diffusion sheet has good luminousness and light diffusion effect, utilizes the diffusion sheet to surround camera and reagent card box, and the light that the diffusion sheet jetted into in reagent card box and the camera to outside refracts, reflects and scatters for even, the incident angle of light on the incident immunochromatography test paper strip is abundant, is favorable to eliminating the shadow of shooing, thereby further promotes the camera and shoots the quality, promotes the degree of accuracy of testing result.
Drawings
FIG. 1 is a right side elevation view of an immunochromatographic device for facilitating addition of a liquid in accordance with one embodiment of the present invention.
Fig. 2 is a schematic view of the right side view in fig. 1.
Fig. 3 is a schematic view of the left side view in fig. 1.
Fig. 4 is a partially enlarged view of a portion a in fig. 2.
Fig. 5 is a partially enlarged view of fig. 3 at B.
Fig. 6 is a schematic view of fig. 1 in a rear-side view.
FIG. 7 is a schematic view of the engagement of a reagent card slot with a reagent cartridge.
Fig. 8 is a partially enlarged view of fig. 2 at C.
Fig. 9 is a partial enlarged view of fig. 6 at D.
FIG. 10 is a schematic view of a reaction-placing plate.
FIG. 11 is a schematic view of a detection mechanism of an immunochromatographic device facilitating addition of a liquid in the second embodiment of the present invention.
Fig. 12 is an exploded view of fig. 11.
Detailed Description
The following is further detailed by way of specific embodiments:
reference numerals in the drawings of the specification include: the device comprises a base 1, a first liquid suction motor 2, a first liquid suction belt 3, a support plate 4, a first sliding block 5, a liquid suction cup 6, a liquid suction needle 7, a hybrid drive pump 8, a second liquid suction motor 9, a second liquid suction belt 10, a second sliding block 11, a liquid suction fixing plate 12, a first reaction belt 13, a reagent clamping groove 14, a first reaction motor 15, a third sliding block 16, a reagent card box 17, a liquid suction needle 18, a waste liquid needle 19, a second reaction motor 20, a second reaction belt 21, a guide shaft 22, a reaction placing plate 23, a clamping through hole 24, a movable groove 25, a waste liquid pump 26, a camera 27, an LED lamp 28, a light diffusion plate 29 and a screw rod 30.
The first embodiment is as follows:
embodiment one referring to fig. 1, fig. 2, fig. 3 and fig. 4, an immunochromatography device for facilitating the addition of liquid comprises a base 1, and a liquid mixing mechanism, a reaction mechanism, a detection mechanism and a control system (not shown) are arranged on the base 1.
With reference to fig. 2 and 3, the liquid mixing mechanism includes a first driving unit provided on the base 1, and the first driving unit includes a liquid suction placing plate, a first liquid suction driving member, and a second liquid suction driving member.
In this embodiment, first imbibition drive spare includes first imbibition motor 2 and connects the first imbibition belt 3 on the output of first imbibition motor 2, through screw fixedly connected with backup pad 4 on the base 1, first imbibition motor 2 passes through bolt fixed connection in backup pad 4, first imbibition belt 3 level sets up and its direction of transfer is along the preceding of figure 1, the rear direction, combine shown in figure 4, first imbibition belt 3 is last to be connected with first slider 5 through screw fixed connection, and be used for the guide arm for first slider 5 direction through screw fixedly connected with in backup pad 4, it places the hole to open on first slider 5, place the imbibition cup 6 that can take at will in the hole, utilize first imbibition motor 2 drive first imbibition belt 3 forward or the antiport, can make imbibition cup 6 along guide bar axle forward, the back slides.
In combination with fig. 2 and 4, the liquid suction cup 6 is provided with two independent mixing chambers capable of containing liquid, a liquid suction needle 7 can be inserted into each mixing chamber, the two liquid suction needles 7 inserted into the two mixing chambers are communicated with each other, the base 1 is connected with a mixing driving member for driving the two mixing chambers to exchange and mix liquid instantly, in this embodiment, the mixing driving member comprises a mixing driving pump 8 fixed on the base 1 through a bolt, and the mixing driving pump 8 is communicated with the liquid suction needles 7.
In this embodiment, referring to fig. 3 and 5, the second liquid suction driving member includes a second liquid suction motor 9 and a second liquid suction belt 10 connected to an output end of the second liquid suction motor 9, the second liquid suction motor 9 is fixedly connected to the base 1 through a screw, the second liquid suction belt 10 is vertically disposed, a second slider 11 is fixedly connected to the second liquid suction belt 10 through a screw, a liquid suction fixing plate 12 is fixedly connected to the second slider 11 through a screw, a fixing hole for fixing the liquid suction needle 7 is formed in the liquid suction fixing plate 12, the second liquid suction motor 9 rotates forwards or reversely to drive the second sliding block 11 to slide vertically upwards and downwards, thereby leading the second slide block 11 to drive the imbibition fixed plate 12 and the imbibition needle 7 to synchronously move up and down, meanwhile, the first liquid suction motor 2 is combined to drive the liquid suction cup 6 to move forwards and backwards, so that the liquid suction needle 7 can be inserted into or withdrawn from the mixing cavity in the liquid suction cup 6.
Referring to fig. 2 and fig. 6, the reaction mechanism includes a first reaction driving member, a first reaction belt 13 and a reagent card slot 14, in this embodiment, the first reaction driving member includes a first reaction motor 15 fixedly connected to the base 1 through a bolt, the first reaction belt 13 is connected to an output end of the first reaction motor 15, the first reaction belt 13 is horizontally disposed, the first reaction belt 13 is fixedly connected to a third sliding block 16 through a bolt, the third sliding block 16 is provided with a card slot for engaging with the reagent card slot 14, and the first reaction belt 13 is driven to drive the third sliding block 16 and the reagent card slot 14 to move horizontally through forward transmission or reverse rotation of the first reaction motor 15.
A reagent card box 17 is clamped in the reagent card slot 14, an immunochromatographic test paper to which a sample to be tested is dripped is placed in the reagent card box 17, as shown in fig. 7, a liquid feeding hole and a waste liquid hole are formed in the reagent card box 17, with reference to fig. 2, a liquid feeding needle 18 and a waste liquid needle 19 are arranged on a transverse sliding path of the reagent card slot 14, with reference to fig. 8 and 9, a second reaction driving member is arranged on the base 1, in this embodiment, the second reaction driving member comprises a second reaction motor 20 fixedly connected to the base 1 through screws, a second reaction belt 21 is connected to an output shaft of the second reaction motor 20, a vertically arranged screw rod 30 is rotatably connected to the base 1 through bearings, a guide shaft 22 arranged in parallel to the screw rod 30 is fixedly connected to the base 1 through screws, a reaction placing plate 23 is vertically slidably connected to the guide shaft 22 through a shaft hole matching manner, a threaded hole matched with the screw rod 30 is formed in the reaction placing plate 23, the second belt is rotated to drive the screw rod 30 to rotate, so that the screw rod 30 drives the reaction placing plate 23 to vertically move.
As shown in fig. 10, the reaction placing plate 23 is provided with holding through holes 24 for holding the liquid feeding needle 18 and the waste liquid needle 19, each holding through hole 24 is provided with an L-shaped movable groove 25, the movable groove 25 penetrates through the reaction placing plate 23, the front end of the L-shaped movable groove 25 is communicated with the front side of the reaction front plate, the rear end of the movable groove 25 is communicated with the holding through hole 24, and after the movable groove 25 is provided, in combination with fig. 8, the aperture of the holding through hole 24 can be enlarged by external force, so that the liquid feeding needle 18 or the waste liquid needle 19 is placed in the holding through hole 24, and after the external force is released, the holding through hole 24 is restored to the original size under the self-restoring force of the reaction placing plate 23, so that the liquid feeding needle 18 or the waste liquid needle 19 can be fixed.
Referring to fig. 2 and 6, the base 1 is fixedly connected with a waste liquid pump 26 communicated with the waste liquid needle 19 through a screw, when the liquid adding needle 18 drops the color developing solution into the reagent cartridge 17, the color developing solution reacts with a sample to be detected on the immunochromatographic test paper, and then the waste liquid pump 26 is used for sucking away the residual waste liquid, so that the color developing solution is prevented from reacting with the immunochromatographic test paper for too much time, and meanwhile, the waste liquid is prevented from influencing the detection of a subsequent detection mechanism.
As shown in fig. 2, the detection mechanism includes a camera 27, the camera 27 is connected to an image analysis system and a storage system (not shown in the figure, for example, an esequal quantitative lateral chromatography detection system developed by tiangen biochemical technology (beijing) ltd, which has analysis and storage functions), the camera 27 is located above the motion track of the reagent cartridge 17, and when the reagent cartridge 17 moves below the camera 27, the camera 27 can photograph the reagent cartridge 17, so as to obtain the reaction result of the immunochromatographic test paper in the reagent cartridge 17.
The specific implementation process is as follows:
1. liquid mixing stage:
when the liquid mixing mechanism is used for mixing liquid, the liquid suction needle 7 moves to the upper limit position under the driving of the second liquid suction motor 9, then the first liquid suction motor 2 is started to transmit positively, the first liquid suction belt 3 drives the first sliding block 5 to slide backwards, so that the first sliding block 5 drives the liquid suction cup 6 to move backwards as shown in figure 2 to the outside of the liquid suction needle 7, and A, B liquid can be conveniently added into two mixing cavities of the liquid suction cup 6 respectively because no shielding object is arranged above the liquid suction cup 6 at the moment; and finally, the first liquid suction motor 2 is reversely rotated to enable the liquid suction cup 6 to move to the front as shown in the figure 2 to the position right below the liquid suction needle 7, then the second liquid suction motor 9 is utilized to drive the second sliding block 11 to move downwards, so that the liquid suction fixing plate 12 fixedly connected to the second sliding block 11 and the liquid suction needle 7 fixed on the liquid suction fixing plate 12 synchronously vertically move downwards, and finally the two liquid suction needles 7 are respectively inserted into the two mixing cavities.
After the liquid suction needle 7 is inserted into the mixing cavity, the mixing driving pump 8 is started, one of the liquid suction needles 7 sucks the liquid (for example, liquid A) in the corresponding mixing cavity into the other mixing cavity through the liquid suction needle 7, so that the A, B liquid is mixed, then the mixing driving pump 8 runs reversely, mixed liquid obtained after A, B liquid mixing is sucked into the mixing cavity originally containing the liquid A, the mixed liquid is continuously sucked and spitted back and forth in the two mixing cavities through the circulating forward and reverse motion of the mixing driving pump 8, and finally the A, B liquid is uniformly mixed to form color developing liquid.
2. A reaction stage:
during reaction, a sample to be detected is placed in the reagent card box 17, then the first reaction motor 15 is used for driving the first reaction belt 13 to rotate, the first reaction belt 13 drives the reagent card slot 14 to move towards the left side as shown in fig. 2, the reagent card box 17 is made to move to the position below the liquid suction needle 7 and the waste liquid needle 19, meanwhile, the liquid adding needle 18 is made to face the liquid adding hole, the waste liquid needle 19 is made to face the waste liquid hole, then the second reaction motor 20 is used for driving the second reaction belt 21 to rotate, the second reaction belt 21 rotates to drive the screw rod 30 to rotate, the screw rod 30 drives the reaction placing plate 23 and the liquid adding needle 18 and the waste liquid needle 19 which are fixed on the reaction placing plate 23 to synchronously vertically move downwards, so that the liquid adding needle 18 is close to the liquid adding hole, and the waste liquid needle 19 is close to the waste liquid hole.
When A, B liquid is uniformly mixed into color development liquid, the color development liquid is driven by a mixing driving pump 8 to enter a liquid adding needle 18, the color development liquid is dripped into a liquid adding hole by the liquid adding needle 18, the color development liquid is contacted with the immunochromatographic test paper to react, the reaction time is timed by a control system, when the preset reaction time is reached, a waste liquid pump 26 is started, the waste liquid in a reagent card box 17 is completely absorbed by a waste liquid needle 19, and then the liquid adding needle 18 and the waste liquid needle 19 are pushed upwards by a second reaction motor 20, so that the liquid adding needle 18 and the waste liquid needle 19 are far away from the reagent card box 17; the first reaction motor 15 is then activated to continue to push the reagent card slot 14 and actual card into the left, so that the reagent card box 17 is positioned directly below the camera 27.
3. A detection stage:
when the reagent card box 17 is positioned below the camera 27, the camera 27 shoots the reagent card box 17 and sends the shot picture to the image analysis system, and the image analysis system automatically analyzes the reaction result of the immunochromatographic test paper in the reagent card box 17 and sends the analysis result to the storage system for storage.
After the shooting is finished, the first reaction motor 15 rotates reversely, the reagent clamping groove 14 and the reagent card box 17 are driven to move rightwards to the right limit position, the reagent card box 17 is drawn out of the reagent clamping groove 14, and therefore the detection of a sample to be detected is finished.
And (3) repeating the operation 2-3 to finish batch sample detection, and repeating the operation 1 to obtain the prepared color developing solution after the color developing solution is used up.
Example two
The difference between the second embodiment and the first embodiment is that: as shown in fig. 11 and 12, a light shielding box is fixedly connected to the base 1 by screws, the camera 27 is fixedly connected to the top of the light shielding box by screws, and a light source is fixedly connected to the light shielding box by screws, in this embodiment, the light source is an LED lamp 28. Be connected with the board 29 that diffuses through screw fixed connection on the shading case, board 29 that diffuses is located between light source and the camera 27, and when reagent card box 17 moved to camera 27 below, board 29 that diffuses can surround camera 27 and reagent card box 17, prevents that external light direct irradiation from reagent card box 17's immunity chromatography test paper and influence the shooting of camera 27.
The specific implementation mode is as follows: compare in embodiment one, when reagent card box 17 moved to camera 27's below to the left, control system control LED lamp 28 is bright, effect through diffusion sheet 29, make camera 27 can obtain a better shooting environment, then utilize camera 27 to shoot reagent card box 17, and send the picture of shooing to image analysis system, image analysis system is to immunochromatography test paper autoanalysis in reagent card box 17, and send the analysis result to storage system and preserve, after setting up LED lamp 28 and diffusion sheet 29, make camera 27's shooting more clear, thereby obtain more accurate testing result, promote sample detection's the degree of accuracy.
The foregoing is merely an example of the present invention and common general knowledge of known specific structures or characteristics of the embodiments is not described herein in any greater detail. It should be noted that, for those skilled in the art, without departing from the structure of the present invention, several changes and modifications can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be defined by the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.
Claims (8)
1. An immunochromatographic device facilitating addition of liquid, comprising a base, characterized in that: the base is provided with a liquid mixing mechanism, a reaction mechanism, a detection mechanism and a control system,
the liquid mixing mechanism is used for automatically mixing and preparing color developing liquid for immunochromatography detection, and comprises a liquid suction cup, two mixing cavities are arranged in the liquid suction cup, liquid suction needles which can be inserted into the mixing cavities and are mutually communicated are arranged in each mixing cavity, and a mixing driving piece for driving the liquids in the two mixing cavities to be instantly exchanged and mixed is connected to each liquid suction needle;
the reaction mechanism is used for obtaining the color developing solution mixed by the solution mixing mechanism and simultaneously enabling the color developing solution to automatically react with the test paper added with the sample to be detected in a sealing manner;
the detection mechanism is used for automatically detecting the reaction result, and analyzing and storing the reaction result;
the control system is used for controlling the operations of liquid mixing, reaction and detection in the immunochromatography detection to be sequentially carried out;
the base is provided with a first driving unit, the first driving unit comprises a liquid absorption placing plate, a first liquid absorption driving part and a second liquid absorption driving part, the liquid absorption cup is detachably connected to the liquid absorption placing plate, the first liquid absorption driving part is used for horizontally driving the liquid absorption placing plate, and the second liquid absorption driving part is used for driving a liquid absorption needle to be inserted into and withdrawn from the mixing cavity;
reaction mechanism still includes first reaction driving piece and second reaction driving piece of fixed connection on the base, first reaction driving piece is used for ordering about the reagent draw-in groove and slides on the base, the board is placed in fixedly connected with reaction on the second reaction driving piece, and liquid feeding needle and waste liquid needle all can be dismantled and connect on the board is placed in the reaction.
2. The immunochromatographic device for facilitating addition of a liquid according to claim 1, wherein: the reaction mechanism comprises a reagent clamping groove connected to the base in a sliding mode, a reagent card box is detachably connected in the reagent clamping groove, a test strip is arranged in the reagent card box, and a liquid feeding hole and a waste liquid hole are formed in the reagent card box; the base is connected with a liquid feeding needle which can be opposite to the liquid feeding hole in a sliding mode, and the liquid feeding needle is communicated with the liquid sucking needle.
3. An immunochromatographic device for facilitating addition of a liquid according to claim 2, wherein: the base is connected with a waste liquid needle capable of facing the waste liquid hole in a sliding mode, the base is fixedly connected with a waste liquid pump communicated with the waste liquid needle, and the waste liquid pump is connected with a control system.
4. An immunochromatographic device for facilitating addition of a liquid according to claim 3, wherein: the reaction is placed and is opened the centre gripping through-hole that is used for placing liquid feeding needle and waste liquid needle on the board, and the reaction is placed and is opened on the board and have the activity groove that is the L type and run through the reaction and place the board, the one end of activity groove is placed the lateral wall of board with the reaction and is link up, the other end and centre gripping through-hole link up.
5. An immunochromatographic device for facilitating addition of a liquid according to claim 4, wherein: the second reaction driving part comprises a second reaction motor fixedly connected to the base, a lead screw is fixedly connected to the second reaction motor, a guide shaft is fixedly connected to the base and arranged in parallel with the lead screw, and a threaded hole matched with the lead screw in a threaded mode and a guide hole matched with the guide shaft are formed in the reaction placing plate.
6. An immunochromatographic device for facilitating addition of a liquid according to claim 4, wherein: the detection mechanism comprises a camera, and an image analysis system and a storage system are connected outside the camera.
7. An immunochromatographic device for facilitating addition of a liquid according to claim 6, wherein: and the base is fixedly connected with a light source for supplementing light to the camera.
8. An immunochromatographic device for facilitating addition of a liquid according to claim 7, wherein: fixedly connected with light scattering plate on the base, light scattering plate is located between light source and the camera, and light scattering plate can surround camera and reagent card box.
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| CN112557678B (en) * | 2020-12-25 | 2024-02-23 | 重庆康巨全弘生物科技有限公司 | Full-automatic chromatographic analyzer |
| CN112557648B (en) * | 2020-12-25 | 2024-04-16 | 重庆康巨全弘生物科技有限公司 | POCT fluorescence immunoassay appearance |
| CN113109554A (en) * | 2021-03-12 | 2021-07-13 | 天津一安生物技术有限公司 | Meat sampling and chromatography detection integrated device |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1492233A (en) * | 2003-09-12 | 2004-04-28 | 四川大学 | Automatic analysis method for COD in sea water |
| CN205374482U (en) * | 2016-01-27 | 2016-07-06 | 广州万孚生物技术股份有限公司 | Full -automatic fluorescent quantitation immunoassay appearance |
| KR20160127876A (en) * | 2015-04-27 | 2016-11-07 | 주식회사 수젠텍 | Device for Detecting Colored Reaction or Fluorescence Reaction of Immunochromatography |
| CN106596989A (en) * | 2017-02-05 | 2017-04-26 | 深圳市活水床旁诊断仪器有限公司 | Full-automatic immunization analyzer and detection method |
| WO2017128806A1 (en) * | 2016-01-27 | 2017-08-03 | 广州万孚生物技术股份有限公司 | Automatic fluorescence quantitative immunoassay analyzer and detection method |
| CN207457261U (en) * | 2017-11-15 | 2018-06-05 | 北京乐普医疗科技有限责任公司 | A kind of full-automatic fluorescence immune chromatography analytical equipment |
| CN108845154A (en) * | 2018-06-25 | 2018-11-20 | 重庆中元汇吉生物技术有限公司 | A kind of immune quantitative analyzer |
| CN109765363A (en) * | 2019-01-25 | 2019-05-17 | 武汉璟泓万方堂医药科技股份有限公司 | Chemiluminescence-immune chromatography test paper and its detection method |
-
2019
- 2019-08-30 CN CN201910815215.1A patent/CN110488002B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1492233A (en) * | 2003-09-12 | 2004-04-28 | 四川大学 | Automatic analysis method for COD in sea water |
| KR20160127876A (en) * | 2015-04-27 | 2016-11-07 | 주식회사 수젠텍 | Device for Detecting Colored Reaction or Fluorescence Reaction of Immunochromatography |
| CN205374482U (en) * | 2016-01-27 | 2016-07-06 | 广州万孚生物技术股份有限公司 | Full -automatic fluorescent quantitation immunoassay appearance |
| WO2017128806A1 (en) * | 2016-01-27 | 2017-08-03 | 广州万孚生物技术股份有限公司 | Automatic fluorescence quantitative immunoassay analyzer and detection method |
| CN106596989A (en) * | 2017-02-05 | 2017-04-26 | 深圳市活水床旁诊断仪器有限公司 | Full-automatic immunization analyzer and detection method |
| CN207457261U (en) * | 2017-11-15 | 2018-06-05 | 北京乐普医疗科技有限责任公司 | A kind of full-automatic fluorescence immune chromatography analytical equipment |
| CN108845154A (en) * | 2018-06-25 | 2018-11-20 | 重庆中元汇吉生物技术有限公司 | A kind of immune quantitative analyzer |
| CN109765363A (en) * | 2019-01-25 | 2019-05-17 | 武汉璟泓万方堂医药科技股份有限公司 | Chemiluminescence-immune chromatography test paper and its detection method |
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