CN113109554A - Meat sampling and chromatography detection integrated device - Google Patents
Meat sampling and chromatography detection integrated device Download PDFInfo
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- CN113109554A CN113109554A CN202110289564.1A CN202110289564A CN113109554A CN 113109554 A CN113109554 A CN 113109554A CN 202110289564 A CN202110289564 A CN 202110289564A CN 113109554 A CN113109554 A CN 113109554A
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- sampling
- needle
- sample
- sample loading
- detection
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- 238000005070 sampling Methods 0.000 title claims abstract description 256
- 238000001514 detection method Methods 0.000 title claims abstract description 151
- 238000004587 chromatography analysis Methods 0.000 title claims abstract description 77
- 235000013372 meat Nutrition 0.000 title claims abstract description 45
- 230000007246 mechanism Effects 0.000 claims abstract description 27
- 239000002904 solvent Substances 0.000 claims description 38
- 238000003825 pressing Methods 0.000 claims description 27
- 238000012360 testing method Methods 0.000 claims description 18
- 238000004873 anchoring Methods 0.000 claims description 14
- 239000003153 chemical reaction reagent Substances 0.000 claims description 12
- 239000003365 glass fiber Substances 0.000 claims description 10
- 238000004108 freeze drying Methods 0.000 claims description 9
- 238000005192 partition Methods 0.000 claims description 9
- 238000007789 sealing Methods 0.000 claims description 2
- 230000009471 action Effects 0.000 description 12
- 238000000034 method Methods 0.000 description 10
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 9
- 235000017491 Bambusa tulda Nutrition 0.000 description 9
- 241001330002 Bambuseae Species 0.000 description 9
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 9
- 239000011425 bamboo Substances 0.000 description 9
- 238000013461 design Methods 0.000 description 8
- 238000010586 diagram Methods 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 238000003317 immunochromatography Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 1
- 238000000861 blow drying Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
Images
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
Abstract
The invention discloses a meat sampling and chromatography detection integrated device, which relates to the technical field of meat detection and comprises the following components: the inner part of the shell is provided with an accommodating cavity, and a guide cylinder is rotatably arranged in the accommodating cavity; the sampling needle is movably arranged in the guide cylinder, one end of the sampling needle is provided with an ejection mechanism, the ejection mechanism can eject the sampling needle out of the sampling port, and a sampling needle sample loading hole is formed in the sampling needle; the chromatography detection structure is arranged on the side wall of the shell; the sample loading needle assembly comprises an ejection cavity and a sample loading needle which is arranged in the ejection cavity in a sliding manner, and the sample loading needle can enter a sample loading hole of the sampling needle and push a sample in the sampling needle into the chromatographic detection structure; the operating sleeve is movably arranged at the other end of the shell, and one end of the operating sleeve is connected with the sampling needle and can drive the correcting cylinder to rotate; the device can realize the swift operation of sampling, appearance and chromatography detection, shortens check-out time.
Description
Technical Field
The invention belongs to the technical field of meat detection, and particularly relates to a meat sampling and chromatography detection integrated device.
Background
In the field of food safety detection, the immunochromatography detection technology is more and more widely applied due to the advantages of rapid detection, intuitive signal response, convenience for field operation and the like. However, for samples containing complex matrices, the relatively complex pre-treatment steps limit further applications of this technique. Taking meat sample detection as an example: as an important part of the resident dietary structure, the meat safety is crucial, and the immunochromatography detection technology can not be used for sample pretreatment in the application of meat detection. The common pretreatment steps comprise the steps of sampling, homogenizing, extracting, blow-drying, redissolving, filtering and the like, and the simplest operation steps also comprise the steps of sampling, homogenizing and filtering. The sample pretreatment not only relates to the use of various professional instruments and equipment, but also needs to consume tens of minutes or even hours, and obviously restricts the application of the rapid detection technology.
Disclosure of Invention
The invention aims to provide a meat sampling and chromatography detection integrated device aiming at the defects in the prior art, the device is provided with a sampling needle, a sampling needle assembly and a chromatography detection structure which are required by meat sampling and chromatography detection, and the rapid operation of sampling, sampling and chromatography detection can be realized by operating an operation sleeve, so that the operation time is greatly shortened, and the application of an immunochromatography detection technology in the rapid detection of meat is promoted.
In order to achieve the above object, the present invention provides an integrated meat sampling and chromatography detection device, comprising:
the sample feeding device comprises a shell, wherein a containing cavity is formed in the shell, a guide cylinder is rotatably arranged in the containing cavity, one end of the shell is provided with a sampling port communicated with the containing cavity, the side wall of the shell is provided with a first sample feeding needle through hole, and the side wall of the guide cylinder is provided with a second sample feeding needle through hole;
the sampling needle is movably arranged in the guide cylinder, one end of the sampling needle is provided with an ejection mechanism, the ejection mechanism can eject the sampling needle out of the sampling port, and a sampling needle sample loading hole is formed in the sampling needle;
the chromatography detection structure is arranged on the side wall of the shell;
the sample loading needle assembly is arranged on the side wall of the shell and comprises an ejection cavity and a sample loading needle which is arranged in the ejection cavity in a sliding manner, and the sample loading needle can enter the sample loading hole of the sampling needle through the first sample loading needle through hole and the second sample loading needle through hole and push a sample in the sampling needle into the chromatography detection structure;
and the operation sleeve is movably arranged at the other end of the shell, and one end of the operation sleeve is connected with the sampling needle and can drive the guide cylinder to rotate.
Optionally, it leads positive groove and second to be provided with first on the inner wall that holds the chamber, first lead positive groove be for following lead the straight flute that the axis direction of just section of thick bamboo set up, the second is led positive groove and is the ring channel, the second lead positive groove with first one end of leading positive groove is connected, lead be provided with on the outer wall of just section of thick bamboo with first lead positive groove with the second is led positive groove sliding fit's and is led positive piece, sampling opening detachably is provided with the shrouding.
Optionally, a sampling needle guide groove is formed in the inner wall of the guide cylinder along the axial direction of the guide cylinder, and the sampling needle includes:
the sampling needle body is cylindrical, a sample containing cavity is formed in the sampling needle body, and a sample loading hole of the sampling needle penetrates through the side wall of the sample containing cavity;
the sampling head is conical and is arranged at one end of the sampling needle body, a sample conveying groove is formed between the edge of one side, close to the sampling needle body, of the sampling head and the sampling needle body, and the sample conveying groove is communicated with the sample containing cavity;
the locking plate is arranged at the other end of the sampling needle body;
the guide bulge is arranged on the outer wall of the sampling needle body and is in sliding fit with the guide groove of the sampling needle.
Optionally, keep away from on the shell one end of sampling port is provided with extends the sleeve, the operation sleeve cover is established extend the sleeve outside, pop-up mechanism includes:
the positioning seat comprises a base part and two elastic clamping pieces, the two elastic clamping pieces are symmetrically arranged on two sides of the base part, the sides of the two elastic clamping pieces close to each other are provided with a first buckling part and a second buckling part, and the second buckling part is arranged at one end, far away from the base part, of each elastic clamping piece and is matched with the locking plate;
the two locking plates are symmetrically arranged at one end of the correcting barrel, a third buckling part matched with the first buckling part is arranged at one end, away from the correcting barrel, of the two locking plates, an inclined plane part is arranged at one end, close to the correcting barrel, of the two locking plates, the inclined plane part is matched with one end, away from the base part, of the elastic clamping piece, a clamping groove is formed in the middle of the second buckling part, and the locking plates are embedded in the clamping groove;
a first spring disposed between the sampling needle and the base portion.
Optionally, a piston rod is slidably arranged inside the sampling needle body, a clamping joint clamped with the other end of the sampling needle body is arranged at one end of the piston rod, and the other end of the piston rod penetrates through the base part and is connected with the operating sleeve; the base part is provided with a reset spring mounting plate, a reset spring mounting groove is formed in the reset spring mounting plate, a reset spring is arranged in the reset spring mounting groove, an anchoring table is arranged on the operation sleeve, the reset spring is connected with the anchoring table, and the anchoring table is in plug-in fit with the reset spring mounting groove.
Optionally, the chromatographic detection structure comprises:
the detection cylinder is internally provided with a detection hole, the detection cylinder is arranged on one side of the shell, and the detection hole is collinear with the axis of the ejection cavity;
the solvent bottle is arranged on one side of the detection cylinder and is communicated with the detection hole, a pressing lug is arranged on one side of the solvent bottle, and the solvent bottle can be extruded by pressing the pressing lug so that the solvent in the solvent bottle enters the detection hole;
and the chromatography test paper clamping groove is arranged on one side of the shell and is communicated with the other side of the detection cylinder.
Optionally, a freeze-drying reagent, a glass fiber mat and a partition board are sequentially arranged in the detection hole from bottom to top, a through hole is formed in the partition board, one side of the detection cylinder is communicated with the solvent bottle through a first capillary tube, the first capillary tube is arranged on one side of the freeze-drying reagent, the other side of the detection cylinder is communicated with the chromatography test paper clamping groove through a second capillary tube, and the second capillary tube is arranged on one side of the glass fiber mat.
Optionally, the loading needle assembly comprises:
the ejection cavity is arranged in the sample loading needle cylinder;
and one end of the second spring is connected with the sample loading needle cylinder, the other end of the second spring is connected with the sample loading needle, and the second spring can push the sample loading needle towards the direction close to the chromatography detection structure.
Optionally, one end of the sample loading needle cylinder is detachably connected with the shell, a detachable cover plate is arranged at the other end of the sample loading needle cylinder, and the second spring is arranged between the cover plate and the sample loading needle.
Optionally, the loading needle comprises:
the sample loading needle body is cylindrical, a concave part is arranged at one end of the sample loading needle body, and a thin-wall structure is formed on the periphery of the concave part;
a plurality of sample pressing columns arranged in the concave part;
and the thread part is arranged at the other end of the sample loading needle body, and the outer diameter of the thread part is larger than that of the sample loading needle body.
The invention provides a meat sampling and chromatography detection integrated device, which has the beneficial effects that:
1. the device is provided with a sampling needle, a sampling needle assembly and a chromatography detection structure which are required by meat sampling and chromatography detection, and can realize quick operation of sampling, sampling and chromatography detection by operating the operation sleeve, so that the meat sampling, sampling and detection are integrated, the operation time is greatly shortened, and the application of an immunochromatography detection technology in the meat quick detection is promoted;
2. the device can realize automatic ejection of the sampling needle for sampling through the arrangement of the guide cylinder and the ejection mechanism, and enables the sampling needle to eject and reset along a set line, and the ejection mechanism can realize ejection and reset of the sampling needle through the design of structures such as a positioning seat, a locking plate and the like, so that multiple sampling is convenient;
3. the device is provided with a sample loading needle assembly capable of being ejected, the initial position of a sample loading needle is limited through the staggered design of a first sample loading needle through hole and a second sample loading needle through hole and a sample loading hole of a sampling needle, the first sample loading needle through hole and the second sample loading needle through hole are aligned to the sample loading hole of the sampling needle through rotating a guide cylinder when sample loading is needed, automatic ejection of the sample loading needle is realized under the action of a second spring, and automatic sample loading is realized;
4. the chromatography detection structure of the device can realize the rapid placement of chromatography test paper by arranging the detection cylinder, the solvent bottle and the chromatography test paper clamping groove, the introduction of a solvent is realized by extruding the solvent bottle after sample loading, and meanwhile, the pretreatment process of the sample before detection is simplified under the sample loading cooperation effect of the sample loading needle, the rapid chromatography detection is realized, and the detection efficiency is improved;
5. sampling and the operation that resets are realized by the operation sleeve in the device use, and the sample need not to contact external environment outside the sampling head, is favorable to avoiding the sample to pollute.
Additional features and advantages of the invention will be set forth in the detailed description which follows.
Drawings
The above and other objects, features and advantages of the present invention will become more apparent by describing in more detail exemplary embodiments thereof with reference to the attached drawings, in which like reference numerals generally represent like parts throughout.
Fig. 1 is a schematic exterior exploded view of an integrated meat sampling and chromatography detection device according to an embodiment of the present invention.
Fig. 2 is an exploded view of an integrated meat sampling and chromatography detection device according to an embodiment of the present invention.
FIG. 3 is a partially exploded view of an integrated meat sampling and chromatography detection device according to an embodiment of the present invention.
FIG. 4 is an exploded view of an integrated meat sampling and chromatography detection device according to an embodiment of the present invention.
FIG. 5 is an exploded view of an integrated meat sampling and chromatography detection device according to an embodiment of the present invention.
Fig. 6 is a schematic structural diagram of a sampling needle of the integrated meat sampling and chromatography detection device according to an embodiment of the invention.
FIG. 7 is a schematic diagram of an operation sleeve of the integrated meat sampling and chromatography detection device according to one embodiment of the invention.
FIG. 8 is a schematic structural diagram of a detection cylinder of the integrated meat sampling and chromatography detection device according to an embodiment of the invention.
FIG. 9 is a schematic structural diagram of a reagent bottle of the integrated meat sampling and chromatography detection device according to an embodiment of the invention.
FIG. 10 is a schematic structural diagram of a housing of an integrated meat sampling and chromatography detection device according to an embodiment of the invention.
FIG. 11 is a schematic structural diagram of a loading needle of an integrated meat sampling and chromatography detection device according to an embodiment of the present invention.
Fig. 12 is an exploded view of a sample loading syringe of an integrated meat sampling and chromatography detection device according to an embodiment of the present invention.
FIG. 13 is an exploded view of the housing of the integrated meat sampling and chromatography detection device, according to an embodiment of the present invention.
Description of reference numerals:
1. a housing; 2. a guide cylinder; 3. a sampling port; 4. a first loading pin through hole; 5. a second loading pin through hole; 6. a sampling needle; 7. an ejection mechanism; 8. sampling holes are formed in the sampling needles; 9. a chromatography detection structure; 10. a sample loading needle assembly; 11. a sample loading needle; 12. an operating sleeve; 13. a first pilot groove; 14. a second pilot groove; 15. a guide block; 16. closing the plate; 17. a sampling needle guide groove; 18. a sampling needle body; 19. a sampling head; 20. a sample delivery slot; 21. a locking plate; 22. a pilot boss; 23. an extension sleeve; 24. positioning seats; 25. a base part; 26. an elastic clip; 27. a first fastening part; 28. a second fastening part; 29. a locking plate; 30. a third buckle part; 31. an inclined plane part; 32. a clamping groove; 33. a first spring; 34. a piston rod; 35. a clamping head; 36. a return spring mounting plate; 37. a return spring mounting groove; 38. a return spring; 39. an anchoring table; 40. a detection cylinder; 41. a solvent bottle; 42. pressing the bump; 43. a chromatography test paper clamping groove; 44. lyophilizing the reagent; 45. a glass fiber mat; 46. a partition plate; 47. a through hole; 48. a first capillary tube; 49. a second capillary tube; 50. a sample loading needle cylinder; 51. a second spring; 52. a cover plate; 53. a sample loading needle body; 54. a recessed portion; 55. pressing a sample column; 56. a threaded portion.
Detailed Description
Preferred embodiments of the present invention will be described in more detail below. While the following describes preferred embodiments of the present invention, it should be understood that the present invention may be embodied in various forms and should not be limited by the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.
The invention provides a meat sampling and chromatography detection integrated device, which comprises:
the sample feeding device comprises a shell, a sampling port, a first sample feeding needle through hole and a second sample feeding needle through hole, wherein a containing cavity is formed in the shell, a guide cylinder is rotatably arranged in the containing cavity, the sampling port communicated with the containing cavity is formed in one end of the shell, the first sample feeding needle through hole is formed in the side wall of the shell, and the second sample feeding needle through hole is formed in the side wall of the guide cylinder;
the sampling needle is movably arranged in the guide cylinder, one end of the sampling needle is provided with an ejection mechanism, the ejection mechanism can eject the sampling needle out of the sampling port, and a sampling needle sample loading hole is formed in the sampling needle;
the chromatography detection structure is arranged on the side wall of the shell;
the sampling needle assembly is arranged on the side wall of the shell and comprises an ejection cavity and a sampling needle which is arranged in the ejection cavity in a sliding manner, the sampling needle can enter the sampling needle sampling hole through the first sampling needle through hole and the second sampling needle through hole and push a sample in the sampling needle into the chromatography detection structure;
and the operation sleeve is movably arranged at the other end of the shell, and one end of the operation sleeve is connected with the sampling needle and can drive the guide cylinder to rotate.
Specifically, the guide cylinder can be operated to rotate in the accommodating cavity through the operation sleeve, so that the first sample loading needle through hole is aligned with or staggered with the second sample loading needle through hole, when the first sample loading needle through hole is aligned with the second sample loading needle through hole, the sample loading needle can move towards the sampling needle through the first sample loading needle through hole and the second sample loading needle through hole, and when the sample loading hole of the sampling needle is aligned with the first sample loading needle through hole and the second sample loading needle through hole, the sample loading needle can enter the sampling needle, and a sample in the sampling needle is pushed into the chromatography detection structure for detection; one end of the sampling needle is matched with the ejection mechanism, the ejection mechanism is controlled by the operation sleeve, the sampling needle can be ejected out through the sampling port under the ejection action of the ejection mechanism, the sampling needle is inserted into the meat to be detected, and the sampling of the sample is completed when the sampling needle is pulled out; the device can automatically complete the sampling, sample loading and detection processes of the sample through the operation of the operation sleeve, and can improve the detection efficiency.
Optionally, be provided with on the inner wall that holds the chamber first lead positive groove and second and lead positive groove, first lead positive groove for the straight flute that sets up along the axis direction of leading positive section of thick bamboo, the second is led positive groove and is the ring channel, the second is led positive groove and is connected with the first one end of leading positive groove, lead be provided with on the outer wall of positive section of thick bamboo with first lead positive groove and second lead positive groove sliding fit lead positive piece, sampling opening detachably is provided with the shrouding.
Specifically, the guide cylinder is matched with the first guide groove and the second guide groove in a sliding mode through the guide block to realize sliding and rotating in the accommodating cavity, the guide cylinder can slide along the first guide groove under the pushing and pulling action of the operation sleeve, the guide cylinder can rotate in the second guide groove under the rotating action of the operation sleeve, and the arrangement of the first guide groove and the second guide groove can limit the moving paths of the guide cylinder and other parts connected with the guide cylinder, so that the normal operation of the ejection mechanism and the sampling needle assembly is guaranteed; the shrouding can avoid the sampling needle to receive the pollution with the shutoff of sampling mouth.
Optionally, the inner wall of leading just section of thick bamboo has been seted up along the axis direction of leading just section of thick bamboo along leading just section of thick bamboo and has been sampled the needle and lead just groove, and the sampling needle includes:
the sampling needle body is cylindrical, a sample containing cavity is formed in the sampling needle body, and a sampling hole in the sampling needle body penetrates through the side wall of the sample containing cavity;
the sampling head is conical and is arranged at one end of the sampling needle body, a sample conveying groove is formed between the edge of one side of the sampling head, which is close to the sampling needle body, and the sample conveying groove is communicated with the sample containing cavity;
the locking plate is arranged at the other end of the sampling needle body;
and the guide bulge part is arranged on the outer wall of the sampling needle body and is in sliding fit with the guide groove of the sampling needle.
Specifically, the sampling needle and the guide cylinder can rotate together due to the matching of the guide bulge and the sampling needle guide groove, in one example, the sampling needle sample loading hole is aligned with the second sample loading needle through hole, and when the guide cylinder rotates, sample loading can be carried out as long as the second sample loading needle through hole is aligned with the first sample loading needle through hole; the sampling head of taper can get into under pop-up effect of pop-up mechanism and wait to detect the sample, and through carrying the operation sleeve this moment, the pulling sampling needle withdraws to the shell in, until will sampling needle resets to the position before popping out, and at the withdrawal in-process, the edge that the sampling head is close to sampling needle body one side can cut the sample, and the sample that is downcut passes through sample conveyer trough and gets into sample and hold the intracavity and store, accomplishes the sampling of sample.
Optionally, the one end of keeping away from the sampling port on the shell is provided with extends the sleeve, and the operation sleeve cover is established in extending the sleeve outside, and pop-up mechanism includes:
the positioning seat comprises a base part and two elastic clamping pieces, the two elastic clamping pieces are symmetrically arranged on two sides of the base part, the sides of the two elastic clamping pieces close to each other are provided with a first buckling part and a second buckling part, and the second buckling part is arranged at one end, away from the base part, of each elastic clamping piece and matched with the locking plate;
the two locking plates are symmetrically arranged at one end of the correcting barrel, a third buckling part matched with the first buckling part is arranged at one end, away from the correcting barrel, of the two locking plates, an inclined plane part is arranged at one end, close to the correcting barrel, of the two locking plates, the inclined plane part is matched with one end, away from the base part, of the elastic clamping piece, a clamping groove is formed in the middle of the second buckling part, and the locking plates are embedded in the clamping groove;
the first spring is arranged between the sampling needle and the base part.
Specifically, the extension sleeve plays a guiding role for the operation sleeve, and meanwhile, the extension sleeve can contain the ejection mechanism; when the sampling needle is in an initial state, a first spring in the pop-up mechanism is in a compressed state, the elastic clamping piece is clamped with the locking plate through the matching of the first clamping part and the third clamping part, and meanwhile, the sampling needle is fixed on the positioning seat through the clamping of the second clamping part and the locking plate; when sampling is needed, the operating sleeve is moved towards the direction close to the shell, the operating sleeve pushes the positioning seat to move, the two elastic clamping pieces are opened due to the sliding fit of the inclined plane parts on the locking pieces and the groove bottoms of the clamping grooves on the elastic clamping pieces, the clamping among the first clamping part, the second clamping part and the third clamping part and the locking plate are unlocked, and the sampling needle is ejected out under the action of the first spring and inserted into a sample; and at the moment, the operation sleeve is pulled back, the operation sleeve drives the sampling needle to move until the sampling needle resets in the pop-up mechanism and clamps the sampling needle again, and the sample is collected into the sampling needle.
Optionally, a piston rod is slidably arranged inside the sampling needle body, one end of the piston rod is provided with a clamping joint clamped with the other end of the sampling needle body, and the other end of the piston rod penetrates through the base part and is connected with the operation sleeve; the base part is provided with a reset spring mounting plate, a reset spring mounting groove is formed in the reset spring mounting plate, a reset spring is arranged in the reset spring mounting groove, an anchoring table is arranged on the operation sleeve, the reset spring is connected with the anchoring table, and the anchoring table is in plug-in fit with the reset spring mounting groove.
It is specific, the joint holds chamber sliding fit with this internal sample of sampling needle to hold the one end joint cooperation of keeping away from the shell in chamber with the sample, operation sleeve drives the piston rod and removes during the pulling operation sleeve, the pulling sampling needle removes, reset until the sampling needle, operation sleeve removes to the direction that is close to the positioning seat under reset spring's effect this moment, piston rod and joint remove to the direction that is close to the sampling head, can hold the sample of intracavity with the sample and push away to sampling needle sample hole department, the convenient appearance of going up.
Optionally, the chromatographic detection structure comprises:
the detection cylinder is internally provided with a detection hole, the detection cylinder is arranged on one side of the shell, and the detection hole is collinear with the axis of the ejection cavity;
the solvent bottle is arranged on one side of the detection cylinder and is communicated with the detection hole, a pressing lug is arranged on one side of the solvent bottle, and the solvent bottle can be extruded by pressing the pressing lug to enable the solvent in the solvent bottle to enter the detection hole;
and the chromatography test paper clamping groove is arranged on one side of the shell and is communicated with the other side of the detection cylinder.
Specifically, the detection section of thick bamboo is installed in one side of shell through detecting a section of thick bamboo mounting groove, and during the appearance of going up, the needle of going up to launch to in the detection hole, push away the sample to in the detection hole, during the detection, through the lug of pressing down solvent bottle one side, can extrude the solvent in the solvent bottle to in the detection hole, then on solvent and sample mix and flow into the chromatography test paper in the chromatography test paper card wipes jointly in the detection hole, can detect through chromatography detection device at last.
Optionally, a freeze-drying reagent, a glass fiber pad and a partition board are sequentially arranged in the detection hole from bottom to top, a through hole is formed in the partition board, one side of the detection cylinder is communicated with the solvent bottle through a first capillary tube, the first capillary tube is arranged on one side of the freeze-drying reagent, the other side of the detection cylinder is communicated with the chromatography test paper clamping groove through a second capillary tube, and the second capillary tube is arranged on one side of the glass fiber pad.
Specifically, a solvent in a solvent bottle enters a detection hole through a first capillary tube, is firstly contacted and mixed with a freeze-drying reagent, then is mixed with a sample pressed by a sample loading needle through a through hole through a glass fiber pad, and then enters chromatographic detection test paper in a chromatographic test paper clamping groove through a second capillary tube; this chromatography detects structure can realize the mixture of sample and solvent and make the chromatography test paper strip automatically through simple operation, conveniently carries out the chromatography detection of sample, can simplify the pretreatment flow that the chromatography detected, effectively improves detection efficiency.
Optionally, the loading needle assembly comprises:
the ejection cavity is arranged in the sample loading needle cylinder;
one end of the second spring is connected with the sample loading needle cylinder, the other end of the second spring is connected with the sample loading needle, and the second spring can push the sample loading needle towards the direction close to the chromatography detection structure.
Specifically, the sample loading needle assembly realizes ejection of the sample loading needle through the elastic force of the second spring, and further realizes automatic sample loading of the device.
Optionally, the one end of going up the appearance cylinder can be dismantled with the shell and be connected, and the other end of going up the appearance cylinder is provided with the detachable apron, and the second spring setting is between apron and the needle of going up the appearance.
Specifically, one end of the sample loading needle cylinder is clamped with the clamping hole in the shell through the clamping structure, so that the sample loading needle cylinder is convenient to disassemble and replace and is convenient for the device to load samples for multiple times; the cover plate can be clamped with the sample loading needle cylinder through the clamping groove, and the disassembly is convenient.
Optionally, the loading needle comprises:
the sample loading needle body is cylindrical, a concave part is arranged at one end of the sample loading needle body, and a thin-wall structure is formed at the periphery of the concave part;
a plurality of sample pressing columns arranged in the concave parts;
and the thread part is arranged at the other end of the sample loading needle body, and the outer diameter of the thread part is larger than that of the sample loading needle body.
Specifically, the periphery of the concave part at one end of the sample loading needle body forms a sharp thin-walled structure, a sample can be cut, the outer ends of the sample pressing columns are pointed ends, sample cuttings can be pushed into the detection hole when the sample loading needle is ejected by the second spring and passes through the sample containing cavity on the sampling needle, and the sample is cut and crushed in the detection hole under the action of the thin-walled structure and the sample pressing columns, so that the mixing of the sample and a solution is facilitated, the pretreatment process of the sample is simplified, and the detection effect is improved; the needle body of drawing a design can be dismantled with the second spring through screw portion and be connected, also can with the fixed welding of second spring, the external diameter that the external diameter of screw portion is greater than the body of drawing a design can be at the in-process restriction of the in-process insertion depth of the needle body of drawing a design to the shell of drawing a design, the needle of drawing a design takes out after the convenient appearance of drawing a design to the next appearance of the device.
Examples
As shown in fig. 1 to 13, the present invention provides an integrated meat sampling and chromatography detection device, comprising:
the sample feeding device comprises a shell 1, wherein a containing cavity is formed in the shell 1, a guide cylinder 2 is rotatably arranged in the containing cavity, one end of the shell 1 is provided with a sampling port 3 communicated with the containing cavity, the side wall of the shell 1 is provided with a first sample feeding needle through hole 4, and the side wall of the guide cylinder 2 is provided with a second sample feeding needle through hole 5;
the sampling needle 6 is movably arranged in the guide cylinder 2, one end of the sampling needle 6 is provided with an ejection mechanism 7, the ejection mechanism 7 can eject the sampling needle 6 from the sampling port 3, and the sampling needle 6 is provided with a sampling needle sample loading hole 8;
the chromatography detection structure 9 is arranged on the side wall of the shell 1;
the sample loading needle assembly 10 is arranged on the side wall of the shell 1 and comprises an ejection cavity and a sample loading needle 11 arranged in the ejection cavity in a sliding manner, and the sample loading needle 11 can enter the sample loading hole 8 of the sampling needle through the first sample loading needle through hole 4 and the second sample loading needle through hole 5 and push a sample in the sampling needle 6 into the chromatographic detection structure 9;
and the operating sleeve 12 is movably arranged at the other end of the shell 1, and one end of the operating sleeve 12 is connected with the sampling needle 6 and can drive the guide cylinder 2 to rotate.
In this embodiment, the inner wall of the accommodating cavity is provided with a first guide groove 13 and a second guide groove 14, the first guide groove 13 is a straight groove arranged along the axis direction of the guide cylinder 2, the second guide groove 14 is an annular groove, the second guide groove 14 is connected with one end of the first guide groove 13, the outer wall of the guide cylinder 2 is provided with a guide block 15 in sliding fit with the first guide groove 13 and the second guide groove 14, and the sampling port 3 is detachably provided with a sealing plate 16.
In this embodiment, the inner wall of the guide cylinder 2 is provided with a sampling needle guide groove 17 along the axial direction of the guide cylinder 2, and the sampling needle 6 includes:
the sampling needle body 18 is cylindrical, a sample containing cavity is formed in the sampling needle body, and a sampling hole 8 in the sampling needle penetrates through the side wall of the sample containing cavity;
the sampling head 19 is conical and is arranged at one end of the sampling needle body 18, a sample conveying groove 20 is formed between the edge of one side, close to the sampling needle body 18, of the sampling head 19 and the sampling needle body 18, and the sample conveying groove 20 is communicated with the sample containing cavity;
a lock plate 21 provided at the other end of the sampling needle body 18;
and the guide bulge part 22 is arranged on the outer wall of the sampling needle body 18, and the guide bulge part 22 is in sliding fit with the sampling needle guide groove 17.
In this embodiment, the end of the housing 1 away from the sampling port 3 is provided with an extension sleeve 23, the operation sleeve 12 is sleeved on the outer side of the extension sleeve 23, and the pop-up mechanism 7 includes:
the positioning seat 24 comprises a base part 25 and two elastic clamping pieces 26, the two elastic clamping pieces 26 are symmetrically arranged on two sides of the base part 25, the sides of the two elastic clamping pieces 26 close to each other are provided with a first buckling part 27 and a second buckling part 28, and the second buckling part 28 is arranged at one end, far away from the base part 25, of the elastic clamping piece 26 and is matched with the locking plate 21;
the two locking plates 29 are symmetrically arranged at one end of the correcting barrel 2, a third buckling part 30 matched with the first buckling part 27 is arranged at one end, away from the correcting barrel 2, of each locking plate 29, an inclined plane part 31 is arranged at one end, close to the correcting barrel 2, of each locking plate 29, the inclined plane part 31 is matched with one end, away from the base part 25, of the elastic clamping piece 26, a clamping groove 32 is formed in the middle of the second buckling part 28, and the locking plates 29 are embedded in the clamping groove 32;
the first spring 33 is provided between the sampling needle 6 and the base portion 25.
In this embodiment, a piston rod 34 is slidably disposed inside the sampling needle body 18, one end of the piston rod 34 is provided with a clamping head 35 clamped with the other end of the sampling needle body 18, and the other end of the piston rod 34 penetrates through the base part 25 to be connected with the operating sleeve 12; the base portion 25 is provided with a return spring mounting plate 36, the return spring mounting plate 36 is provided with a return spring mounting groove 37, the return spring mounting groove 37 is internally provided with a return spring 38, the operating sleeve 12 is provided with an anchoring table 39, the return spring 38 is connected with the anchoring table 39, and the anchoring table 39 is in plug-in fit with the return spring mounting groove 37.
In the present embodiment, the chromatography detection structure 9 includes:
the detection cylinder 40 is internally provided with a detection hole, the detection cylinder 40 is arranged on one side of the shell 1, and the detection hole is collinear with the axis of the ejection cavity;
a solvent bottle 41 which is arranged at one side of the detection cylinder 40 and is communicated with the detection hole, a pressing lug 42 is arranged at one side of the solvent bottle 41, and the solvent bottle 41 can be extruded by pressing the pressing lug 42 so that the solvent in the solvent bottle 41 enters the detection hole;
and the chromatography test paper clamping groove 43 is arranged on one side of the shell 1 and is communicated with the other side of the detection cylinder 40.
In this embodiment, a freeze-drying reagent 44, a glass fiber mat 45 and a partition 46 are sequentially disposed in the detection hole from bottom to top, a through hole 47 is formed in the partition 46, one side of the detection cylinder 40 is communicated with the solvent bottle 41 through a first capillary tube 48, the first capillary tube 48 is disposed at one side of the freeze-drying reagent 44, the other side of the detection cylinder 40 is communicated with the chromatography test paper slot 43 through a second capillary tube 49, and the second capillary tube 49 is disposed at one side of the glass fiber mat 45.
In this embodiment, the loading needle assembly 10 includes:
the sample loading needle cylinder 50 is provided with an ejection cavity inside the sample loading needle cylinder 50;
and a second spring 51 having one end connected to the loading syringe 50 and the other end connected to the loading needle 11, wherein the second spring 51 is capable of pushing the loading needle 11 in a direction approaching the chromatographic detection structure 9.
In this embodiment, one end of the sample application cylinder 50 is detachably connected to the housing 1, the other end of the sample application cylinder 50 is provided with a detachable cover plate 52, and the second spring 51 is disposed between the cover plate 52 and the sample application needle 11.
In the present embodiment, the loading needle 11 includes:
a sample application needle body 53 which is cylindrical, wherein one end of the sample application needle body 53 is provided with a concave part 54, and the periphery of the concave part 54 forms a thin-wall structure;
a plurality of sample-pressing columns 55 provided in the recessed portion 54;
and a thread part 56 provided at the other end of the sampling needle body 53, wherein the outer diameter of the thread part 56 is larger than that of the sampling needle body 53.
In summary, the action process of the meat sampling and chromatography detection integrated device provided by the invention can be summarized as "one pressing, one lifting, one rotating and one pressing" and corresponds to different states of the device, and the operation is as follows:
1. initial state
Referring to fig. 5, wherein the sampling needle 6 is in a locked state due to the engagement of the locking plate 21 and the second locking portion 28, the first locking portion 27 and the third locking portion 30 are in snap-fit engagement to guide and position the sampling needle 6, and the first spring 33 is in a compressed state; the second spring 51 in the ejection cavity is in a compressed state, and the elastic force of the second spring 51 acts on the sample loading needle 11; the chromatography detection structure 9 is embedded in a corresponding mounting groove at one side of the shell 1;
2. sample Collection State ('one pressure')
By applying pressure to the operating sleeve 12, the positioning seat 24 drives the two elastic clamping pieces 26 to move towards the direction close to the housing 1 through the force transmission action, under the action of the inclined plane part 31 of the locking piece 29, the two elastic clamping pieces 26 are opened outwards, so that the second buckling part 28 is separated from the locking plate 21, the first buckling part 27 is separated from the third buckling part 30, and under the action of the elastic force of the first spring 33, the sampling needle 6 is popped out from the sampling port 3 into a sample to be measured;
3. reset state ('one handle')
After the sampling needle 6 enters a sample to be detected, the piston rod 34 connected with the sampling needle is driven by pulling the operation sleeve 12, the clamping joint 35 of the piston rod 34 acts on the end part of the sampling needle 6 and is clamped, and the sampling needle 6 retracts towards the inside of the shell 1 until the locking state of the sampling needle 6 in the initial state is recovered by pulling the piston rod 34; in the retraction process of the sampling needle 6, a sample cut by the sharp edge of the sampling head 19 enters a sample containing cavity in the sampling needle body 18 along the sample conveying groove 20, so that the sample is collected; then the operating sleeve 12 is released, and under the action of the return spring 38, the operating sleeve 12 is restored to the initial position, and meanwhile, the clamping joint 35 on the piston rod 34 pushes the sample in the sample containing cavity to one end close to the sampling hole 8 of the sampling needle;
4. sample loading state (one turn)
After the operation sleeve 12 is reset, the operation sleeve 12 is rotated to drive the positioning seat 24 to rotate through the matching of the anchoring table 39 and the reset spring installation groove 37, the positioning seat 24 drives the guide cylinder 2 to rotate through the matching of the clamping groove 32 and the locking plate 29 until the sampling needle sample loading hole 8 is coaxial with the first and second sampling needle through holes 5; at this time, the first spring 33 pushes the sample loading needle 11 to pass through the first and second sample loading needle through holes 5 and further to enter the sampling needle 6 through the sampling needle sample loading hole 8, and under the action of the thin-wall structure at the front end of the sample loading needle 11 and the plurality of sample pressing columns 55, the sample is separated, crushed and delivered into the detection hole;
5. detecting state ('one pressing')
After sampling, the pressing bump 42 is pressed to extrude the solvent bottle 41, the solvent enters the detection hole through the first capillary tube 48, the freeze-dried reagent 44 is dissolved and mixed with the sample, and then the solvent reaches the chromatography detection test paper in the chromatography test paper clamping groove 43 through the second capillary tube 49, and finally integrated rapid detection is realized.
The device improves sample pretreatment efficiency in order to promote the application of immunochromatography detection technology in meat detection, shortens the time from sampling to detection, and adopts the following technical measures: firstly, the sampling needle 6 which can be popped out through the pop-up mechanism 7 is arranged, so that the sampling efficiency is improved, and the pop-up device and the guide structure on the guide cylinder 2 can ensure that the sampling needle 6 is realized according to a set path in the initial, pop-up and reset states; secondly, the pop-up mechanism 7 realizes the functions of positioning, unlocking, resetting and the like through the matching of the plurality of buckling parts, is convenient for sampling for a plurality of times, has a simple structure and is beneficial to saving the cost; thirdly, the sample loading device also adopts an ejection mode to load samples, the sample loading needle 11 can realize the crushing of the samples in the sample loading process, and the processing efficiency of the samples is improved; fourthly, the arrangement of the chromatography detection device is matched with the sample loading needle assembly 10, so that integrated rapid detection can be realized.
Having described embodiments of the present invention, the foregoing description is intended to be exemplary, not exhaustive, and not limited to the embodiments disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the described embodiments.
Claims (10)
1. The utility model provides a meat sampling and chromatography detect integrated device which characterized in that, the device includes:
the sample feeding device comprises a shell, wherein a containing cavity is formed in the shell, a guide cylinder is rotatably arranged in the containing cavity, one end of the shell is provided with a sampling port communicated with the containing cavity, the side wall of the shell is provided with a first sample feeding needle through hole, and the side wall of the guide cylinder is provided with a second sample feeding needle through hole;
the sampling needle is movably arranged in the guide cylinder, one end of the sampling needle is provided with an ejection mechanism, the ejection mechanism can eject the sampling needle out of the sampling port, and a sampling needle sample loading hole is formed in the sampling needle;
the chromatography detection structure is arranged on the side wall of the shell;
the sample loading needle assembly is arranged on the side wall of the shell and comprises an ejection cavity and a sample loading needle which is arranged in the ejection cavity in a sliding manner, and the sample loading needle can enter the sample loading hole of the sampling needle through the first sample loading needle through hole and the second sample loading needle through hole and push a sample in the sampling needle into the chromatography detection structure;
and the operation sleeve is movably arranged at the other end of the shell, and one end of the operation sleeve is connected with the sampling needle and can drive the guide cylinder to rotate.
2. The meat sampling and chromatography detection integrated device of claim 1, wherein a first guide groove and a second guide groove are arranged on the inner wall of the accommodating cavity, the first guide groove is a straight groove arranged along the axis direction of the guide cylinder, the second guide groove is an annular groove, the second guide groove is connected with one end of the first guide groove, a guide block in sliding fit with the first guide groove and the second guide groove is arranged on the outer wall of the guide cylinder, and the sampling port is detachably provided with a sealing plate.
3. The meat sampling and chromatography detection integrated device of claim 1, wherein a sampling needle guide groove is formed in the inner wall of the guide cylinder along the axial direction of the guide cylinder, and the sampling needle comprises:
the sampling needle body is cylindrical, a sample containing cavity is formed in the sampling needle body, and a sample loading hole of the sampling needle penetrates through the side wall of the sample containing cavity;
the sampling head is conical and is arranged at one end of the sampling needle body, a sample conveying groove is formed between the edge of one side, close to the sampling needle body, of the sampling head and the sampling needle body, and the sample conveying groove is communicated with the sample containing cavity;
the locking plate is arranged at the other end of the sampling needle body;
the guide bulge is arranged on the outer wall of the sampling needle body and is in sliding fit with the guide groove of the sampling needle.
4. The meat sampling and chromatography detection integrated device of claim 3, wherein an extension sleeve is disposed at one end of the housing away from the sampling port, the operation sleeve is sleeved outside the extension sleeve, and the ejection mechanism comprises:
the positioning seat comprises a base part and two elastic clamping pieces, the two elastic clamping pieces are symmetrically arranged on two sides of the base part, the sides of the two elastic clamping pieces close to each other are provided with a first buckling part and a second buckling part, and the second buckling part is arranged at one end, far away from the base part, of each elastic clamping piece and is matched with the locking plate;
the two locking plates are symmetrically arranged at one end of the correcting barrel, a third buckling part matched with the first buckling part is arranged at one end, away from the correcting barrel, of the two locking plates, an inclined plane part is arranged at one end, close to the correcting barrel, of the two locking plates, the inclined plane part is matched with one end, away from the base part, of the elastic clamping piece, a clamping groove is formed in the middle of the second buckling part, and the locking plates are embedded in the clamping groove;
a first spring disposed between the sampling needle and the base portion.
5. The meat sampling and chromatography detection integrated device according to claim 4, wherein a piston rod is slidably arranged inside the sampling needle body, one end of the piston rod is provided with a clamping joint clamped with the other end of the sampling needle body, and the other end of the piston rod penetrates through the base part and is connected with the operation sleeve; the base part is provided with a reset spring mounting plate, a reset spring mounting groove is formed in the reset spring mounting plate, a reset spring is arranged in the reset spring mounting groove, an anchoring table is arranged on the operation sleeve, the reset spring is connected with the anchoring table, and the anchoring table is in plug-in fit with the reset spring mounting groove.
6. The integrated meat sampling and chromatographic detection device according to claim 1, wherein the chromatographic detection structure comprises:
the detection cylinder is internally provided with a detection hole, the detection cylinder is arranged on one side of the shell, and the detection hole is collinear with the axis of the ejection cavity;
the solvent bottle is arranged on one side of the detection cylinder and is communicated with the detection hole, a pressing lug is arranged on one side of the solvent bottle, and the solvent bottle can be extruded by pressing the pressing lug so that the solvent in the solvent bottle enters the detection hole;
and the chromatography test paper clamping groove is arranged on one side of the shell and is communicated with the other side of the detection cylinder.
7. The meat sampling and chromatography detection integrated device of claim 6, wherein a freeze-drying reagent, a glass fiber mat and a partition plate are sequentially arranged in the detection hole from bottom to top, a through hole is formed in the partition plate, one side of the detection cylinder is communicated with the solvent bottle through a first capillary tube, the first capillary tube is arranged on one side of the freeze-drying reagent, the other side of the detection cylinder is communicated with the chromatography test paper clamping groove through a second capillary tube, and the second capillary tube is arranged on one side of the glass fiber mat.
8. The integrated meat sampling and chromatography detection device of claim 1, wherein the sample needle assembly comprises:
the ejection cavity is arranged in the sample loading needle cylinder;
and one end of the second spring is connected with the sample loading needle cylinder, the other end of the second spring is connected with the sample loading needle, and the second spring can push the sample loading needle towards the direction close to the chromatography detection structure.
9. The meat sampling and chromatography detection integrated device of claim 8, wherein one end of the sample loading needle cylinder is detachably connected with the housing, a detachable cover plate is arranged at the other end of the sample loading needle cylinder, and the second spring is arranged between the cover plate and the sample loading needle.
10. The integrated meat sampling and chromatography detection device of claim 1, wherein the sample loading needle comprises:
the sample loading needle body is cylindrical, a concave part is arranged at one end of the sample loading needle body, and a thin-wall structure is formed on the periphery of the concave part;
a plurality of sample pressing columns arranged in the concave part;
and the thread part is arranged at the other end of the sample loading needle body, and the outer diameter of the thread part is larger than that of the sample loading needle body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110289564.1A CN113109554A (en) | 2021-03-12 | 2021-03-12 | Meat sampling and chromatography detection integrated device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110289564.1A CN113109554A (en) | 2021-03-12 | 2021-03-12 | Meat sampling and chromatography detection integrated device |
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| CN113109554A true CN113109554A (en) | 2021-07-13 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202110289564.1A Pending CN113109554A (en) | 2021-03-12 | 2021-03-12 | Meat sampling and chromatography detection integrated device |
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