CN109765363A - Chemiluminescence-immune chromatography test paper and its detection method - Google Patents
Chemiluminescence-immune chromatography test paper and its detection method Download PDFInfo
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- CN109765363A CN109765363A CN201910071113.3A CN201910071113A CN109765363A CN 109765363 A CN109765363 A CN 109765363A CN 201910071113 A CN201910071113 A CN 201910071113A CN 109765363 A CN109765363 A CN 109765363A
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Abstract
Chemiluminescence-immune chromatography test paper, comprising: the chromatographic test paper with shell and with the matching used liquid-feeding tube of chromatographic test paper, liquid-feeding tube is provided with the solution for reaction after being added dropwise on test paper and chemiluminescence colour developing.Its advantage is that: 1, the structure design of of the present invention chromatographic test paper gathered the convenience of immune chromatography test paper, and with the chemiluminescent jamproof advantage of high sensitivity；2, test paper and liquid-feeding tube are used cooperatively in the present invention, can be understood luminous situation immediately, be improved the sensitivity of diagnosis, easy to operate, at low cost；3, the detection that shines is carried out by chemical reagent method in the present invention, cooperates shine multiplier tube, timer etc., light intensity can be read automatically, and understood luminous intensity and detect the concentration relationship of substance, quantitative detection can be carried out to sample to be tested.
Description
Technical field
The present invention relates to field of medical examination, specifically a kind of chemiluminescence-immune chromatography test paper and its detection side
Method.
Background technique
Immune chromatography test paper common at present is colloidal gold, latex or fluorescence.But its sensitivity and anti-interference are not changed
It is advantageous to learn luminescence method.
Common chemiluminescence at present is then mainly wet process then solution reaction in test tube, there is the advantages of high sensitivity,
But the requirement to equipment is relatively high, also unsuitable single part operation；
Chromatographic test paper is colloid gold label or fluorescent marker, needs additionally to give certain light source, colloidal gold when detecting
For visible light, fluorescence is exciting light.These light are issued by LED light, and the difference being easy to appear between lamp and lamp is then led
Cause the difference of testing result.
Summary of the invention
The object of the present invention is to provide a kind of chemiluminescence-immune chromatography test paper and its detection methods, make it after the reaction
Self-luminescence is not necessarily to additional light source, and has gathered immune chromatography test paper convenience, and has had both chemiluminescent highly sensitive anti-
The advantage of interference improves the sensitivity of diagnostic kit.
First aspect present invention protects a kind of chemiluminescence-immune chromatography test paper, comprising: the chromatographic test paper with shell and
With the matching used liquid-feeding tube of chromatographic test paper, liquid-feeding tube is provided with for reacting simultaneously chemiluminescence colour developing after being added dropwise on test paper
Solution.
Second aspect of the present invention protects the detection method of the first aspect chromatographic test paper, comprising the following steps:
S1, sample to be tested is added dropwise on chromatographic test paper, when sample is positive, compound can be generated in detection line；
S2, pressing liquid-feeding tube so that in liquid-feeding tube solution mixing after instill chromatographic test paper sample pad on, and to inhale sample
It is mobile to pad direction, shines after being reacted with the compound in detection line；
S3, the step S2 chromatographic test paper to shine is put into the magazine of fluorescence detecting system, is then turned on power switch, timing
Device starts timing, collects the light in detection line by photomultiplier tube, and by the control of cpu pcb, display screen shows phase
Information is closed, the content that standard curve calculates determined antigen is drawn.
A kind of chemiluminescence-immune chromatography test paper and its detection method, its advantage is that:
1, the convenience of immune chromatography test paper has been gathered in the structure design of chromatographic test paper of the present invention, and with chemiluminescence
The jamproof advantage of high sensitivity；
2, test paper and liquid-feeding tube are used cooperatively in the present invention, can be understood luminous situation immediately, be improved the sensitive of diagnosis
Degree, it is easy to operate, at low cost；
3, the detection that shines is carried out by chemical reagent method in the present invention, cooperates shine multiplier tube, timer etc., it can be automatic
The light intensity on test paper is read, and understands luminous intensity and detects the concentration relationship of substance, quantitative detection can be carried out to sample to be tested.
Detailed description of the invention
Fig. 1 is chemiluminescence-immune chromatography test paper assembling schematic diagram；
Fig. 2 is the part side structure schematic view that chromatographic test paper is arranged in interior of shell；
Fig. 3 is that the test paper after present invention drop sample carries out the part side structure schematic view detected that shines；
Fig. 4 is the circuit connection diagram of detection structure in the method for the present invention；
Wherein:
Shell 1, well 11, detection form 12, sample suction pad form 13；
Chromatographic test paper 2, sample pad 21, nitrocellulose filter 22, sample suction pad 23, bottom plate 24；
Liquid-feeding tube 3, press-side 31, dropping liquid end 32, liquid storage chamber 33, pricker 34；
Power switch 4, timer 5, photomultiplier tube 6, cpu pcb 7, display screen 8.
Specific embodiment
According to Fig. 1: chemiluminescence-immune chromatography test paper, comprising: the chromatographic test paper 2 with shell 1 and with chromatography try
The matching used liquid-feeding tube 3 of paper 2, liquid-feeding tube 3 are provided with the solution for reaction after being added dropwise on test paper and chemiluminescence colour developing.
Preferably, the chromogenic reaction reagent that the solution in the liquid-feeding tube 3 is two kinds or more；
The upper and lower end parts of the liquid-feeding tube 3 are respectively press-side 31 and dropping liquid end 32, the press-side 31 and dropping liquid end 32
Between be disposed with the liquid storage chambers 33 of multiple independent storage chromogenic reaction reagents, the inner face of the press-side 31 is disposed with pricker 34,
Multiple liquid storage chambers 33 and dropping liquid end 32 are punctured simultaneously for disposable, and can quickly be mixed；
The main body of the liquid-feeding tube 3 is cylinder, and lower body portion is tapered in an inverted cone for dropping liquid end 32, the liquid-feeding tube
Multiple liquid storage chambers 33 in 3 are arranged in parallel along axial slices.
Further, as shown in Figure 2 and Figure 3: shell 1 includes the well 11 successively opened up, detection form 12, sample suction pad
Form 13, well 11 is corresponding with the sample pad 21 on chromatographic test paper 2, nitrocellulose on detection form 12 and chromatographic test paper 2
The coated detection line of film 22, nature controlling line are corresponding, and sample suction pad form 13 is corresponding with the sample suction pad 23 on chromatographic test paper 2, for observing
Whether sample reaches sample suction pad 23, when prepare liquid is by after the reaction was completed, passing through liquid-feeding tube on the instillation chromatographic test paper 2 of well 11
3 the chromophoric solution that shines is added dropwise in the sample pad 21 under well 11, so that detection line, nature controlling line develop the color.
Further, 32 aperture of dropping liquid end and 11 size of well are coincide.
Liquid-feeding tube 3 is soft material, convenient for squeezing.
Further, the sample pad 21 on chromatographic test paper 2, nitrocellulose filter 22, sample suction pad 23 successively overlap, and nitric acid
Overlapped that length is 1~2cm with sample pad 21, sample suction pad 23 respectively at cellulose membrane 22 both ends；
Sample pad 21, nitrocellulose filter 22, sample suction pad 23 are adhered on bottom plate 24, and bottom plate 24 is PVC adhesive sticker
Backer board.Inside the fixing of chromatographic test paper 2 and shell 1.
The antibody 1 (or antigen) of acridinium ester or luminous catalysis enzyme label is coated in sample pad 21；And sample pad 21 is
Glass fibre membrane；
Antibody 2, secondary antibody have been drawn respectively in detection line, nature controlling line on nitrocellulose filter 22.
A kind of detection method of chemiluminescence-immune chromatography test paper, comprising the following steps:
S1, sample to be tested is added dropwise on chromatographic test paper 2, when sample is positive, compound can be generated in detection line；
S2, pressing liquid-feeding tube 3, so that the solution in liquid-feeding tube 3 instills in the sample pad 21 of chromatographic test paper 2 after mixing, and
It is mobile to 23 direction of sample suction pad, it shines after being reacted with the compound in detection line；
S3, the step S2 chromatographic test paper to shine is put into the magazine of fluorescence detecting system, is then turned on power switch 4, counts
When device 5 start timing, the light in detection line is collected by photomultiplier tube 6, and pass through the control of cpu pcb 7, display screen 8
It shows relevant information, draws the content that standard curve calculates determined antigen.
Photomultiplier tube 6, cpu pcb 7 are set in magazine；
Preferably, in step S1, when the antibody 1 for being coated with acridinium ester label in sample pad 21, nitrocellulose filter 22
Coated antibody 2 in detection line generate fixed antibody 1- antigen in the detection line of nitrocellulose filter 22 when sample is positive
Sample-acridinium ester label antibody 1 compound；In step S2, what is stored respectively in two liquid storage chambers 33 of liquid-feeding tube 3 is
50mM sodium hydroxide solution, 100 μ l, 0.9% hydrogen peroxide of 100 μ of μ l~1000 l；
Preferably, in step S1, when the antibody (or antigen) for being coated with alkali phosphatase enzyme mark in sample pad 21, nitric acid is fine
Coated antibody 2 in the detection line of plain film 22 is tieed up, when sample is positive, generates solid phase packet in the detection line of nitrocellulose filter 22
By antibody-determined antigen-enzymic-labelled antibody compound；In step S2, a liquid storage chamber 33 storage of liquid-feeding tube 3 is AMPPD
(3- (2'- spiral adamantane) -4- methoxyl group -4- (3 "-phosphinylidyne oxygroup) benzene -1,2- dioxetane) luminous agent buffer,
Alkaline phosphatase makes AMPPD slough phosphate groups and shine；
Preferably, in step S1, when sprayed in sample pad 21 useful horseradish peroxidase (HRP) label antibody (or
Antigen), after being immunoreacted with the sample and solid phase carrier to be measured in reaction system, it is to be measured to form solid-phase coating antibody-
Antigen-enzyme (HRP) labelled antibody compound；In step S2, what is stored respectively in three liquid storage chambers 33 of liquid-feeding tube 3 is luminol
Luminous agent, H2O2, luminescence enhancer；
Further, luminescence enhancer is preferably to iodophenol.
Temporal information is set on cpu pcb 7, and timer 5 carries out timing, while when display screen 8 intuitively shows correlation
Between information, after reaching the time, power switch 4 carry out it is corresponding open or close, control photomultiplier tube 6, which enters, working condition or to stop
Only work；After reaching the setting reaction time, chromophoric solution is added, detection line, nature controlling line light intensity after reaction can be read automatically,
The luminous intensity is related to the detection concentration of substance.
The working principle of photomultiplier tube 6 is photoelectric effect, is that two second sons is recycled to double on the basis of photoelectric effect
Phenomenon and it is manufactured.
Power switch 4, timer 5, photomultiplier tube 6, cpu pcb 7, the composition Test paper glazed thread of display screen 8 are strong
The detection structure of degree, connection schematic diagram are as shown in Figure 4.
Unspecified part herein is the prior art, therefore is not repeated them here.For example, the test paper after shining is carried out
Detection, to draw the content etc. that standard curve calculates determined antigen.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all in spirit of the invention and
Within principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (9)
1. chemiluminescence-immune chromatography test paper, it is characterised in that: tried including the chromatographic test paper (2) with shell (1) and with chromatography
Paper (2) matching used liquid-feeding tube (3), liquid-feeding tube (3) are provided with for reaction after being added dropwise on test paper and chemiluminescence colour developing
Solution.
2. chemiluminescence-immune chromatography test paper according to claim 1, it is characterised in that: molten in the liquid-feeding tube (3)
The chromogenic reaction reagent that liquid is two kinds or more.
3. chemiluminescence-immune chromatography test paper according to claim 2, it is characterised in that: above and below the liquid-feeding tube (3)
End is respectively press-side (31) and dropping liquid end (32), is disposed with multiple independences between the press-side (31) and dropping liquid end (32)
The liquid storage chamber (33) of chromogenic reaction reagent is stored, the inner face of the press-side (31) is disposed with pricker (34), for disposable same
When puncture multiple liquid storage chambers (33) and dropping liquid end (32).
4. chemiluminescence-immune chromatography test paper according to claim 3, it is characterised in that: the main body of the liquid-feeding tube (3)
For cylinder, lower body portion it is tapered it is in an inverted cone be dropping liquid end (32), multiple liquid storage chambers (33) edge in the liquid-feeding tube (3)
Axial slices parallel arrangement.
5. chemiluminescence-immune chromatography test paper according to claim 3, it is characterised in that: shell (1) includes successively opening up
Well (11), detection form (12), sample suction pad form (13), the sample pad on well (11) and chromatographic test paper (2)
(21) corresponding, detection form (12) is corresponding with the coated detection line of nitrocellulose filter (22), nature controlling line on chromatographic test paper (2),
Sample suction pad form (13) is corresponding with sample suction pad (23) on chromatographic test paper (2), for observing whether sample reaches sample suction pad (23),
When prepare liquid is instilled on chromatographic test paper (2) after the reaction was completed by well (11), by liquid-feeding tube (3) under well (11)
Sample pad (21) on be added dropwise shine chromophoric solution so that detection line, nature controlling line develop the color.
6. any chemiluminescence-immune chromatography test paper detection method according to claim 1~5, it is characterised in that: packet
Include following steps
S1, sample to be tested is added dropwise on chromatographic test paper (2), when sample is positive, compound can be generated in detection line；
S2, pressing liquid-feeding tube (3), so that the solution in liquid-feeding tube (3) instills the sample pad (21) of chromatographic test paper (2) after mixing
On, and it is mobile to sample suction pad (23) direction, it shines after being reacted with the compound in detection line；
S3, the step S2 chromatographic test paper to shine is put into the magazine of fluorescence detecting system, is then turned on power switch (18), timing
Device (16) starts timing, collects the light in detection line by photomultiplier tube (14), and pass through the control of cpu pcb (15),
Display screen (17) shows relevant information, draws the content that standard curve calculates determined antigen.
7. the detection method of chemiluminescence-immune chromatography test paper according to claim 6, it is characterised in that: in step S1,
When coated antibody 2, sample on the antibody 1 for being coated with acridinium ester label on sample pad (21), the detection line of nitrocellulose filter (22)
When this is positive, fixed antibody 1- antigen sample-acridinium ester label antibody 1 is generated in the detection line of nitrocellulose filter (22)
Compound；In step S2, that store respectively in two liquid storage chambers (33) of liquid-feeding tube (3) is the 50mM of 100 μ of μ l~1000 l
Sodium hydroxide solution, 100 μ l0.9% hydrogen peroxide.
8. the detection method of chemiluminescence-immune chromatography test paper according to claim 6, it is characterised in that: in step S1,
It is coated with when on the antibody or antigen for being coated with alkali phosphatase enzyme mark on sample pad (21), the detection line of nitrocellulose filter (22)
Antibody 2 generates solid-phase coating antibody-determined antigen-enzyme mark in the detection line of nitrocellulose filter (22) when sample is positive
Remember antibody complex；In step S2, a liquid storage chamber 33 storage of liquid-feeding tube (3) is AMPPD luminous agent buffer, alkaline phosphorus
Sour enzyme makes AMPPD slough phosphate groups and shine.
9. the detection method of chemiluminescence-immune chromatography test paper according to claim 6, it is characterised in that: in step S1,
When the antibody or antigen for spraying useful horseradish peroxidase-labeled on sample pad (21), with the sample to be measured in reaction system
After being immunoreacted with solid phase carrier, solid-phase coating antibody-determined antigen-enzymic-labelled antibody compound is formed；In step S2,
What is stored respectively in three liquid storage chambers (33) of liquid-feeding tube (3) is luminol luminous agent, H2O2, luminescence enhancer.
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Cited By (3)
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| CN110488002A (en) * | 2019-08-30 | 2019-11-22 | 重庆康巨全弘生物科技有限公司 | A kind of device for immunochromatography convenient for adding liquid |
| CN112834747A (en) * | 2021-03-03 | 2021-05-25 | 山东康华生物医疗科技股份有限公司 | Kit for rapidly detecting neutralizing antibody of novel coronavirus |
| CN115097151A (en) * | 2022-06-22 | 2022-09-23 | 福州大学 | Full-automatic chemiluminescence immunochromatography detector and working method thereof |
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Application publication date: 20190517 |