CN217033968U - Portable household POCT lateral chromatography detection device - Google Patents

Abstract

The utility model provides a portable lateral chromatography detection device which comprises a detection shell, wherein the detection shell is a cavity with an opening at the top, the detection shell forms a first cavity and a second cavity through a separation plate, the bottom of the separation plate is provided with a through hole, and the first cavity is communicated with the second cavity through the through hole. The reagent bottle is arranged in the first cavity and filled with detection reagents or sample treatment liquid, the bottom of the reagent bottle is sealed through a plastic packaging film, and a pricking bottle structure used for pricking the plastic packaging film is arranged at the bottom of the first cavity. The second cavity is internally provided with a detachable lateral chromatography detection test strip which can be used for detecting substances such as antigens, antibodies, nucleic acids and the like in a sample. The utility model has the following beneficial effects: the utility model can be used for rapidly detecting the lateral chromatography, and the kit has reasonable structural layout, good portability and strong practicability.

Description

Portable household POCT lateral chromatography detection device

Technical Field

The utility model relates to the technical field of lateral chromatography detection, in particular to a portable household POCT lateral chromatography detection device which is reasonable in structure and convenient to detect and operate.

Background

The lateral chromatography technology is a novel in vitro diagnosis technology developed in the 90 s of the 20 th century on the basis of a monoclonal antibody technology, a colloidal gold immunochromatography technology and a new material technology, has the advantages of rapidness, simplicity, single detection and economy, and is widely applied to the fields of medical detection, food quality monitoring, environment monitoring, agriculture and animal husbandry, entry and exit inspection and quarantine, forensic case setting and the like. The lateral chromatography technology uses a microporous filter membrane (NC membrane) with large aperture as a carrier, fixes specific binders (antigen, antibody, avidin and the like) on the NC membrane, when a sample to be detected is added on a sample pad at one end of a test strip, the sample moves laterally through capillary action, and generates specific binding reaction with a reagent marked by colloidal gold or microspheres on the binding pad, and then the sample moves on the NC membrane, is captured by the binders fixed on the surface of the NC membrane and is gathered on a detection strip, and an intuitive color development result is obtained by visually observing the density of light reflection signals of markers (colloidal gold or latex particles) on the surface of cellulose nitrate. Other unbound labels cross the detection zone and flow into the absorbent pad, so that the purpose of automatic separation is achieved, the existing lateral chromatography is applied to detection in various occasions, but the existing lateral chromatography detection equipment is not portable enough, and the equipment needs to be operated by professionals, so that the quick, convenient or household operation requirements of people cannot be met.

SUMMERY OF THE UTILITY MODEL

In order to solve the defects of the prior art, the utility model provides a portable household POCT lateral chromatography detection device.

The purpose of the utility model is realized by the following technical scheme:

the utility model provides a portable domestic POCT lateral flow chromatography detection device, includes detects the shell, it is provided with open-ended cavity for the top to detect the shell, it passes through the division board and forms first cavity and second cavity to detect the shell, first cavity with second cavity bottom intercommunicates each other.

Preferably, the detachable is provided with the reagent bottle in the first cavity, be equipped with detachable side direction chromatography test paper strip in the second cavity, be provided with detect reagent or sample treatment fluid in the reagent bottle or wait to examine the sample.

Preferably, the detection device comprises a retractable sampling rod inserted into the reagent bottle, and of course, the sampling rod can also be non-retractable, such as: a nasopharyngeal swab, or a sampling wand for other uses. The sampling rod can be arranged separately and combined with the device when in use.

Preferably, the reagent bottle includes the reagent bottle body and sets up the bottle lid in reagent bottle body top, the sampling stick with the bottle lid is for dismantlement formula or integral type connection.

Preferably, the bottom of the reagent bottle is of a pierceable structure, and the bottom of the first cavity is provided with a piercing bottle structure for piercing the bottom of the reagent bottle.

Preferably, an auxiliary cover for covering the second cavity is arranged on one side of a bottle cap of the reagent bottle, and a tearable protective ring is arranged around the bottom of the bottle cap.

Preferably, a circle of transition bulges are convexly arranged on the inner wall of the first cavity and are arranged above the thorn bottle structure.

Preferably, the outer diameter of the reagent bottle is equivalent to the inner diameter of the transition bulge.

Preferably, the lateral surface of second cavity opens has the window that is used for observing or detects, thereby the test result is judged to the visual window available naked eye observation or instrument detection.

Preferably, the portable domestic POCT lateral chromatography detection device is used for lateral chromatography detection, and the detection fields include but are not limited to antigen detection, antibody detection, nucleic acid detection and other ligands.

The utility model has the following beneficial effects: the kit can be used for quickly detecting the immunochromatography, and has the advantages of reasonable structural layout, good portability and strong practicability.

Drawings

FIG. 1: the embodiment of the utility model is a schematic diagram of an explosion structure.

FIG. 2: the reagent of the utility model is a schematic structure after assembly.

Wherein, 1 first cavity, 2 second cavities, 3 reagent bottles, 31 plastic envelope membrane, 11 transition arch, 12 thorn bottle structures, 41 sampling stick, 42 supplementary lid, 43 tearable guard circle, 4 bottle lids, 5 side direction chromatography test paper strips, C control line, T reaction line, 13 windows.

Detailed Description

The technical scheme of the utility model is described in the following by combining the embodiment and fig. 1 and 2, and the utility model discloses a portable household POCT lateral chromatography detection device which comprises a detection shell, wherein the detection shell is a cavity with an opening at the top. In this embodiment, the detection housing may be made of any material.

The detection shell forms a first cavity 1 and a second cavity 2 through a separation layer, the first cavity 1 is communicated with the bottom of the second cavity 2, and the separation layer can be in a whole plate form or a separation strip form. When the whole plate form is adopted, the bottom of the whole plate is provided with the communicating hole. The reagent bottle 3 is detachably arranged in the first cavity 1, and a detection reagent is arranged in the reagent bottle 3. The specific components of the detection reagent can be selected and replaced according to the items to be detected.

The reagent bottle 3 has a height corresponding to the first chamber 1. The bottom of the reagent bottle 3 is sealed through a plastic packaging film 31, and a protruding bottle puncturing structure 12 used for puncturing the plastic packaging film is arranged at the bottom of the first cavity. The material of the plastic film 31 is not limited herein. A circle of transition protrusions 11 are convexly arranged on the inner wall of the first cavity 1, and the transition protrusions 11 are arranged above the thorn bottle structure 12. The outside diameter of the reagent bottle 3 corresponds to the inside diameter of the transition projection 11. Reagent bottle 3 with transition arch 11 is clearance fit, controls well tolerance between them, also can be interference fit, and final purpose is through pressing the upper end of reagent bottle 3, reagent bottle 3 will make the bottom of reagent bottle 3 and thorn bottle structure 12 butt through transition arch 11. When the bottom of the reagent bottle 3 is pierced, the reagent will pass through the first chamber 1 and enter the second chamber 2. Of course, the transition protrusion 11 may also be in a convex point form to achieve the preliminary limit function. The thorn bottle structure in this embodiment is a protrusion with a sharp top end. The bottom of the reagent bottle 3 can also adopt other forms of pierceable structures, and is not limited to a plastic packaging film.

A lateral chromatography test strip 5 is detachably inserted into the second cavity 2. The detection principle of the test strip is the same as that of the lateral chromatography test strip on the market, and the detected objects comprise but are not limited to antigens, antibodies, nucleic acids and the like. The accommodating cavity of the second cavity 2 is adapted to the specification and size of the existing test strip on the market. A window 13 is further formed in the outer side face of the second cavity 2, so that results can be conveniently checked. The window 13 may be any hollow or transparent window convenient for observation, such as a circle, an ellipse, a square, a U-shape, etc.

The kit further comprises a sampling wand 41 for insertion into the reagent bottle 3. Specifically, reagent bottle 3 includes the reagent bottle body and sets up bottle lid 4 in reagent bottle body top, sampling stick 41 with bottle lid 4 is for dismantlement formula or integral type connection, sampling stick 41 form also can be different according to different sample types, telescopic that can be as an organic whole, or non-telescopic. The sampling wand 41 may terminate in a threaded port or a cotton swab.

In this embodiment, the sampling rod 41 is screwed with the reagent bottle 3 through the bottle cap 4. In order to avoid the sampling rod 41 from contacting with the reagent in the reagent bottle 3 before sampling, the threaded opening of the reagent bottle 3 is provided with a plastic package. The threaded port can also adopt other forms, for example, the reagent in the reagent bottle 3 is directly subjected to plastic package in a plastic package manner inside the reagent bottle, and then the sampling rod 41 is inserted into the reagent bottle to be mixed with the reagent.

An auxiliary cover 42 for covering the second cavity is arranged on one side of the bottle cap 4 of the reagent bottle, and a protection ring 43 capable of being torn is arranged around the bottom of the bottle cap 4. The auxiliary lid 42 may ensure cleanliness within the second chamber 2. The tearable protective ring 43 is constructed and arranged in a manner similar to the tamper-evident ring of the prior art bottle cap. The tearable protection ring 43 can prevent the reagent bottle from being pressed to the bottom of the first cavity by mistake. Before pressing, the tearable protection ring 43 needs to be torn off, and then pressed toward the first cavity.

The following illustrates the principle of use of the utility model:

the sample to be tested is collected by the sampling wand 41 which is then inserted into the reagent bottle 3 to mix with the reagent. Pressing reagent bottle 3 to first cavity 1 bottom, pricking the plastic envelope membrane of reagent bottle 3 bottom through pricking bottle structure 12 of bottom, flowing into second cavity 2 from the through-hole through the reagent of mixing with the sample that awaits measuring, it can to detect test paper's sample pad contact reagent this moment. And after a period of time, observing the detection result of the sample to be detected through the result of the test paper.

The utility model can rapidly detect the sample without mastering complex equipment operation, is more suitable for operation of families or ordinary people and has strong practicability.

The following is illustrated with reference to specific use examples:

example 1: novel coronavirus antibody detection reagent (immunochromatography)

The method comprises the following operation steps:

s1, a sample to be tested (nasopharyngeal swab or other nucleic acid amplification test solution from a sample) is taken in the reagent bottle through the sampling rod 41.

S2, shaking the reagent bottle to fully disperse the sample;

s3, pressing the reagent bottle to enable the bottle puncturing structure 12 at the bottom of the first cavity 1 to puncture the plastic packaging film 31 at the bottom of the reagent bottle, enabling the sample liquid in the bottle to flow into the sample pad of the test strip in the second cavity, judging the result within 20 minutes, and displaying the result invalid after 20 minutes.

And interpreting the result through a window.

Positive: a colored band appears on the reaction line T and the control line C at the same time, which indicates that the specimen has a new coronavirus antibody;

Negative: a colored band appears on the control line C, and a colorless band appears on the reaction line (T), which indicates that the specimen has no new coronavirus antibody;

And (4) invalidation: if no color band appears on the control line C and the reaction line T, or a color band appears on the reaction line T, the test is invalid, and a new test paper is applied for retesting.

Example 2: stool occult blood detection (immunochromatography)

The operation steps are as follows:

s1, taking out the sampling rod 41 and taking a stool sample;

s2, inserting the sampling rod 41 into the reagent bottle 3;

s3, shaking the reagent bottle 3 to fully disperse the sample, and waiting for 3 minutes to make the sample liquid uniform;

s4, pressing the reagent bottle to make the pricking bottle structure 12 at the bottom of the first cavity prick the film at the bottom of the reagent bottle, so that the sample liquid in the bottle flows into the sample pad of the test strip in the second cavity, the result is interpreted within 10 minutes, and the result is invalid after 10 minutes.

And interpreting the result through a window.

Positive: a colored tape appears on the reaction line T and the control line C at the same time, which indicates that the specimen has recessive bleeding;

Negative: a colored band appears on the control line C, and a colorless band appears on the reaction line T, which indicates that the specimen has no occult bleeding;

And (4) invalidation: if no color band appears on the control line C and the reaction line T, or a color band appears on the reaction line T, the test is invalid, and a new test paper is applied for retesting.

Of course, that there are many other specific embodiments of the utility model, and that they are not to be taken as examples herein. All technical solutions formed by using equivalent substitutions or equivalent transformations fall within the scope of the claimed invention.

Claims (9)

1. The utility model provides a portable domestic POCT lateral flow chromatography detection device which characterized in that: including detecting the shell, it is provided with open-ended cavity for the top to detect the shell, it passes through the separate layer and forms first cavity and second cavity to detect the shell, first cavity with second cavity bottom UNICOM each other.

2. The portable home-use POCT lateral flow assay device of claim 1, wherein: the detachable in the first cavity is provided with a reagent bottle, be equipped with detachable side direction chromatography test paper strip in the second cavity, be provided with detect reagent or sample treatment fluid in the reagent bottle, or wait to examine the sample.

3. A portable home POCT lateral flow assay device as claimed in claim 2, wherein: the detection device comprises a telescopic sampling rod inserted in the reagent bottle.

4. A portable home POCT lateral flow assay device as claimed in claim 3, wherein: the reagent bottle includes the reagent bottle body and sets up in the bottle lid of reagent bottle body top, the sampling stick with the bottle lid is for dismantlement formula or integral type connection.

5. The portable home-use POCT lateral flow assay device of claim 4, wherein: the bottom of the reagent bottle is of a pierceable structure, and the bottom of the first cavity is provided with a pierceable bottle structure for piercing the bottom of the reagent bottle.

6. The portable home-use POCT lateral flow assay device of claim 5, wherein: an auxiliary cover used for covering the second cavity is arranged on one side of the bottle cap of the reagent bottle, and a protection ring capable of being torn is arranged around the bottom of the bottle cap.

7. The portable home-use POCT lateral flow assay device of claim 5, wherein: the inner wall of the first cavity is convexly provided with a circle of transition bulges, and the transition bulges are arranged above the thorn bottle structure.

8. The portable home-use POCT lateral flow assay device of claim 7, wherein: the outer diameter of the reagent bottle is equivalent to the inner diameter of the transition bulge.

9. The portable home-use POCT lateral flow assay device of claim 8, wherein: and a window for observation or detection is formed on the outer side surface of the second cavity.

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