CN110476721B - Device and method for infesting hepialus larva - Google Patents
Device and method for infesting hepialus larva Download PDFInfo
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- CN110476721B CN110476721B CN201910738540.2A CN201910738540A CN110476721B CN 110476721 B CN110476721 B CN 110476721B CN 201910738540 A CN201910738540 A CN 201910738540A CN 110476721 B CN110476721 B CN 110476721B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/50—Inoculation of spawn
Abstract
The invention discloses a device and a method for infecting hepialus larvae, wherein the device comprises an infecting box, a socket A, a socket B, a partition board, a partition net and a net cover; the infection box is a box body with a cuboid structure, and the upper end of the infection box is provided with an opening; one side surface of the infection box is horizontally provided with a strip-shaped socket A and a strip-shaped socket B, and the socket B is positioned above the socket A; the partition board is of a rectangular sheet-shaped sheet structure and can be inserted into the socket B; the partition net is a rectangular sheet-shaped net piece which can be inserted into the socket A; after the partition plate and the partition net are inserted into the jack B and the jack A, the infection box is divided into an upper space, a middle space and a lower space; the net cover is of a cuboid structure with an opening at the lower end and composed of net sheets, and the net cover is covered at the opening at the upper end of the infection box.
Description
Technical Field
The invention relates to the technical field of hepialus larva infection, in particular to a hepialus larva infection device using cordyceps sinensis and a hepialus larva infection method using the device.
Background
Hepialus is the main host insect of Cordyceps sinensis, and the life habit of adults mainly breeds the offspring through mating, spawning and other reproductive activities. The hepialus life cycle is as long as 4 years or more than 4 years, the larva period is as long as more than 3 years, the adult period is shorter, about 25-28 days, and the individual survival period is generally 3-4 days. Cordyceps sinensis invades host hepialus larva in 7 and 8 months each year, and the nutrition of the larva is fully utilized to reproduce hyphae so as to fill the whole body cavity. The infected larvae are drilled into the soil for overwintering for about 10 months, and the larvae die to become stiff insects when the ground temperature is 2-9 ℃, and the stiffness degree is higher as the ground temperature is continuously reduced. At the dehiscence line of the stiff larva head, a sub-seat starts to grow. The growth of the sub-base is very slow after the earth temperature is in the range of 1.8-2.2 ℃ from 11 months to 2 months next year. When the temperature rises to 5.5 ℃ in the late 4 months, the stroma grows rapidly. At the beginning of 5 months, the ice and snow melt, the soil layer is thawed, and the sub-base drills out surface soil. The seed bed which is unearthed grows fastest a few days before, and the seed bed is brown in color for 10-20 days, and the top begins to expand to produce ascus fruits. After 25 days the stroma stopped growing. After 48 days, the linear spores are ripe, the underground insects decay, and the stroma begins to wilt. The upper part of the cordyceps fungus sporophore expands into a cylindrical sub-seat, the lower part of the cordyceps fungus sporophore is connected with the head of the cordyceps fungus sporophore through a sub-seat handle, a plurality of ascus shells are arranged in the sub-seat, ascus are arranged in the ascus shells, each ascus contains 2-8 ascospores, and the sub-seat which is uneaten of creates favorable conditions for the propagation and invasion of ascospores to insects;
the natural cordyceps sinensis is slow in the process of infecting the hepialus larva, the hepialus larva is often threatened by other external species in the natural environment, the probability of successfully infecting the hepialus larva by the cordyceps sinensis is reduced, and the formation of cordyceps sinensis is small.
Disclosure of Invention
The invention provides a device for infecting hepialus larva by using cordyceps sinensis and a method for infecting hepialus larva by using the device, which solve the problems of reduced probability of successful infection of hepialus larva by cordyceps sinensis and less formation of cordyceps sinensis in natural environment.
In order to achieve the purpose of the invention, the invention adopts the following technical scheme:
the device for infecting hepialus larva comprises an infecting box, a socket A, a socket B, a partition board, a partition net and a net cover; the infection box is a box body with a cuboid structure, and the upper end of the infection box is opened; one side surface of the infection box is horizontally provided with a strip-shaped socket A and a strip-shaped socket B, and the socket B is positioned above the socket A; the partition board is of a rectangular sheet-shaped thin plate structure and can be inserted into the socket B; the partition net is a rectangular sheet net piece which can be inserted into the socket A; the partition board and the partition net are inserted into the jack B and the jack A, and then divide the infection box into an upper space, a middle space and a lower space; the net cover is of a cuboid structure with an opening at the lower end and composed of net sheets, and the net cover is opened at the upper end of the infection box; playing a role in preventing mosquitoes.
Preferably, one side of the partition board is provided with a partition board handle; one side of the separation net is provided with a separation net handle.
Preferably, the length of the infection box is 60cm, the width is 30cm, and the height is 30cm.
Preferably, the socket A is positioned at a position 20cm away from the bottom of the infection box on the side surface of the infection box; the distance between the socket A and the socket B is 5cm.
Preferably, the net cover is 60cm long, 30cm wide and 5cm high.
The method for infecting the hepialus larva by using the device for infecting the hepialus larva comprises the following steps of:
s1: placing fungus soil with Cordyceps sinensis into the bottom of an infection box with a height of 20cm, and placing a plurality of hepialus larvae into the fungus soil;
s2: inserting a spacer net, dicing carrot and paving the diced carrot on the spacer net to form a nutrition layer with the thickness of 5cm;
s3: uniformly mixing coconut coir, turfy soil and plateau soil, sterilizing at 120deg.C for 30min, and oven drying to humidity of less than 10%; then spreading the material on a separator with the thickness of 2-5cm;
s4: and (5) pulling out the partition board, and after 10 times, pulling out the partition net after the cordyceps sinensis does not infect the hepialus larva.
Compared with the prior art, the invention has the following beneficial effects:
the device has the advantages that the Cordyceps sinensis is infected with the hepialus larva more quickly and conveniently through the three-layer structure, and compared with the infection efficiency of the hepialus larva in the cumin environment, the device has higher infection efficiency, and the efficiency of artificially culturing the Cordyceps sinensis is improved; the fungus soil layer, the nutrition layer and the dry soil layer are orderly distributed through structures such as a partition plate and a partition net, so that obvious differences of the forms of the cultivated cordyceps sinensis due to external factors are avoided; the provided screen structure can enable the lower larva to eat food on the upper nutrition layer, and limit the movement space of the hepialus larva in a certain time, so that the Cordyceps sinensis can completely infect the hepialus larva.
Drawings
Fig. 1 is a schematic diagram of a device for infesting hepialus larvae;
in the figure: infection box 1, socket A11, socket B12, baffle 2, baffle handle 21, baffle net 3, baffle net handle 31, net lid.
Detailed Description
The drawings in the embodiments of the present invention will be combined; the technical scheme in the embodiment of the invention is clearly and completely described:
as shown in fig. 1, a device for infesting hepialus larvae comprises a infesting tank 1, a socket A11, a socket B12, a partition board 2, a partition net 3 and a net cover 4; the infection box 1 is a box body with a cuboid structure, and the upper end of the infection box is open; one side surface of the infection box 1 is horizontally provided with a strip-shaped socket A11 and a strip-shaped socket B12, and the socket B12 is positioned above the socket A11; the partition board 2 is of a rectangular sheet-shaped thin plate structure and can be inserted into the insertion opening B12; the partition net 3 is a rectangular sheet-shaped net sheet which can be inserted into the socket A11; after the partition plate 2 and the partition net 3 are inserted into the inserting ports B12 and A11, the infection box 1 is divided into an upper space, a middle space and a lower space; the net cover is of a cuboid structure with an opening at the lower end and composed of net sheets, and the net cover 4 covers the opening at the upper end of the infection box 1; plays a role in preventing mosquitoes, and is particularly implemented; placing fungus soil with Cordyceps sinensis in the bottommost space, placing hepialus larva in the fungus soil, and placing hepialus larva food in the middle space; the upper layer is covered with a layer of dry soil; then sequentially pulling out the partition plate 2 and the partition net 3; forming a fungus soil layer, a nutrition layer and a dry soil layer in the box body; is used for infecting hepialus larva to form Cordyceps.
Preferably, a partition handle 21 is arranged on one side of the partition 2; one side of the separation net 3 is provided with a separation net handle 31; the partition board 2 and the partition net 3 are conveniently pulled out.
Preferably, the infection box 1 is 60cm long, 30cm wide and 30cm high.
Preferably, the socket A11 is positioned at a position 20cm away from the bottom of the infection box 1 on the side surface of the infection box 1; the distance between the socket A11 and the socket B12 is 5cm, so that the fungus soil layer is 20cm and the nutrition layer is 5cm; the dry soil layer is 2-5cm.
Preferably, the net cover 4 is 60cm long, 30cm wide and 5cm high; guaranteeing the air permeability and preventing external mosquitoes.
In a first embodiment of the method of infesting hepialus larvae with Cordyceps sinensis using one of the above embodiments, the method comprises the steps of:
s1: placing fungus soil with Cordyceps sinensis into the bottom of the infection box 1 with the height of 20cm, and placing a plurality of hepialus larvae into the fungus soil;
s2: inserting a separation net 3, dicing carrots and paving the carrots on the separation net 3 to form a nutrition layer with the thickness of 5cm;
s3: uniformly mixing coconut coir, turfy soil and plateau soil, sterilizing at 120deg.C for 30min, and oven drying to moisture of 9%; then spread it on the baffle plate 2, the thickness is 2cm; the dry soil layer prevents the nutrition layer from mildew and has better air permeability;
s4: the partition plate 2 is pulled out, after 10 times, when the cordyceps sinensis is not infected by hepialus larva, the partition net 3 is pulled out; the net 3 can enable the lower larva to eat food of the upper nutrition layer, and limit the movement space of the hepialus larva in a certain time, so that the hepialus larva is prevented from climbing out of the fungus soil layer, and the Cordyceps sinensis cannot completely infect the hepialus larva;
in a second embodiment of the method of infesting hepialus larvae with Cordyceps sinensis using one of the above embodiments of the apparatus for infesting hepialus larvae, comprising the steps of:
s1: placing fungus soil with Cordyceps sinensis into the bottom of the infection box 1 with the height of 20cm, and placing a plurality of hepialus larvae into the fungus soil;
s2: inserting a separation net 3, dicing carrots and paving the carrots on the separation net 3 to form a nutrition layer with the thickness of 5cm;
s3: uniformly mixing coconut coir, turfy soil and plateau soil, sterilizing at 120deg.C for 30min, and oven drying to moisture of 7%; then spread it on the baffle plate 2, the thickness is 3cm; the dry soil layer prevents the nutrition layer from mildew and has better air permeability;
s4: the partition plate 2 is pulled out, after 10 times, when the cordyceps sinensis is not infected by hepialus larva, the partition net 3 is pulled out; the net 3 can enable the lower larva to eat food of the upper nutrition layer, and limit the movement space of the hepialus larva in a certain time, so that the hepialus larva is prevented from climbing out of the fungus soil layer, and the Cordyceps sinensis cannot completely infect the hepialus larva;
in a third embodiment of the method of infesting hepialus larvae with Cordyceps sinensis using one of the above embodiments, the method comprises the steps of:
s1: placing fungus soil with Cordyceps sinensis into the bottom of the infection box 1 with the height of 20cm, and placing a plurality of hepialus larvae into the fungus soil;
s2: inserting a separation net 3, dicing carrots and paving the carrots on the separation net 3 to form a nutrition layer with the thickness of 5cm;
s3: uniformly mixing coconut coir, turfy soil and plateau soil, sterilizing at 120deg.C for 30min, and oven drying to humidity of 5%; then spread it on the baffle plate 2, the thickness is 5cm; the dry soil layer prevents the nutrition layer from mildew and has better air permeability;
s4: the partition plate 2 is pulled out, after 10 times, when the cordyceps sinensis is not infected by hepialus larva, the partition net 3 is pulled out; the screen 3 can enable the lower larva to eat food on the upper nutrition layer, and limit the movement space of the hepialus larva in a certain time, so that the hepialus larva is prevented from climbing out of the fungus soil layer, and the Cordyceps sinensis cannot completely infect the hepialus larva.
Claims (1)
1. A device for infecting hepialus larvae comprises an infecting box, a socket A, a socket B, a partition board, a partition net and a net cover; the method is characterized in that: the infection box is a box body with a cuboid structure, and the upper end of the infection box is opened; one side surface of the infection box is horizontally provided with a strip-shaped socket A and a strip-shaped socket B, and the socket B is positioned above the socket A; the partition board is of a rectangular sheet-shaped thin plate structure and can be inserted into the socket B; the partition net is a rectangular sheet net piece which can be inserted into the socket A; the partition board and the partition net are inserted into the jack B and the jack A, and then the infection box is divided into an upper space, a middle space and a lower space; the net cover is of a cuboid structure with an opening at the lower end and composed of net sheets, and the net cover is opened at the upper end of the infection box; a partition handle is arranged on one side of the partition; one side of the separation net is provided with a separation net handle; the length of the infection box is 60cm, the width of the infection box is 30cm, and the height of the infection box is 30cm; the socket A is positioned at a position 20cm away from the bottom of the infection box on the side surface of the infection box; the distance between the socket A and the socket B is 5cm; the length of the net cover is 60cm, the width of the net cover is 30cm, and the height of the net cover is 5cm; a method for infecting hepialus larvae, which comprises the following steps,
s1: placing fungus soil with Cordyceps sinensis into the bottom of an infection box with a height of 20cm, and placing a plurality of hepialus larvae into the fungus soil;
s2: inserting a spacer net, dicing carrots and paving the diced carrots on the spacer net to form a nutrition layer with the thickness of 5cm; inserting a partition board;
s3: uniformly mixing coconut coir, turfy soil and plateau soil, sterilizing at 120deg.C for 30min, and oven drying to humidity of less than 10%; then spreading the material on a separator with the thickness of 2-5cm;
s4: and (5) pulling out the partition board, and pulling out the partition net after the Cordyceps sinensis infects the hepialus larva.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910738540.2A CN110476721B (en) | 2019-08-12 | 2019-08-12 | Device and method for infesting hepialus larva |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910738540.2A CN110476721B (en) | 2019-08-12 | 2019-08-12 | Device and method for infesting hepialus larva |
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| Publication Number | Publication Date |
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| CN110476721A CN110476721A (en) | 2019-11-22 |
| CN110476721B true CN110476721B (en) | 2023-10-13 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005237201A (en) * | 2004-02-24 | 2005-09-08 | Tohaku Nosan Kigyo Kumiai | Artificial cultivation method for cordyceps sinensis sacc |
| CN207678485U (en) * | 2017-12-29 | 2018-08-03 | 重庆市中药研究院 | A kind of two-phase film reactor of bat moth larvae inoculum bacterium source |
| CN207754165U (en) * | 2018-01-17 | 2018-08-24 | 甘肃农业大学 | A kind of simple seedling fungal infection device |
| CN210987410U (en) * | 2019-08-12 | 2020-07-14 | 北京同仁堂健康药业（青海）有限公司 | Bat moth larva infects device |
-
2019
- 2019-08-12 CN CN201910738540.2A patent/CN110476721B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005237201A (en) * | 2004-02-24 | 2005-09-08 | Tohaku Nosan Kigyo Kumiai | Artificial cultivation method for cordyceps sinensis sacc |
| CN207678485U (en) * | 2017-12-29 | 2018-08-03 | 重庆市中药研究院 | A kind of two-phase film reactor of bat moth larvae inoculum bacterium source |
| CN207754165U (en) * | 2018-01-17 | 2018-08-24 | 甘肃农业大学 | A kind of simple seedling fungal infection device |
| CN210987410U (en) * | 2019-08-12 | 2020-07-14 | 北京同仁堂健康药业（青海）有限公司 | Bat moth larva infects device |
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| CN110476721A (en) | 2019-11-22 |
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