CN110452035A - For preventing and treating the organic fertilizer and its method of administration of citrus bacterial canker disease - Google Patents
For preventing and treating the organic fertilizer and its method of administration of citrus bacterial canker disease Download PDFInfo
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- CN110452035A CN110452035A CN201910843333.3A CN201910843333A CN110452035A CN 110452035 A CN110452035 A CN 110452035A CN 201910843333 A CN201910843333 A CN 201910843333A CN 110452035 A CN110452035 A CN 110452035A
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- Prior art keywords
- organic fertilizer
- citrus
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- preventing
- canker disease
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Pest Control & Pesticides (AREA)
- Soil Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Agronomy & Crop Science (AREA)
- General Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Fertilizers (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to citrus planting technology fields, and in particular to for preventing and treating the organic fertilizer and its method of administration of citrus bacterial canker disease；Including following raw material components: organic fertilizer carrier, Bacillus subtilis and Trichoderma harzianum；A kind of for preventing and treating the method for administration of the organic fertilizer of citrus bacterial canker disease, winter applies fertilizer, and compounds application 30cm wide after microbial bacterial agent organic fertilizer is mixed with soil according to 3:1, in the deep organic fertilizer ditch of 40cm, and in surface blinding；This method prevents and treats microbial bacterial agent applied to citrus bacterial canker disease using organic fertilizer as carrier, and specifies additive amount of the microbial bacterial agent in organic fertilizer carrier, have the advantages that the lasting period is long, resistance risk is small, application is simple and have citrus growth-promoting concurrently.
Description
Technical field
The invention belongs to citrus planting technology fields, and in particular to for preventing and treating organic fertilizer and its application of citrus bacterial canker disease
Method.
Background technique
Citrus is global first big fruit and the fifth-largest trade agricultural product, and China is one of the major country of citrus plantation,
2015 year end China's citrus total cultivated areas are up to 37,700,000 mu, 36,600,000 tons of yield.Citrus bacterial canker disease is a kind of yellow single by carpet
Citrus bacterial disease caused by spore bacillus citrus pvs oryzae and oryzicola (Xanthomonas citri subsp.Citri, Xcc).Mandarin orange
The harm of tangerine canker will lead to citrus early leaf fall, fallen flowers, and citrus tree vigo(u)r is caused to fail, and fruit yield is reduced, and quality reduces, shadow
Ring the income of citrus farmer.Report there is citrus bacterial canker disease in each main citrus growing area of China at present,
Very huge economic loss is caused to the especially orange class of Citrus Industry and shaddock class industry, it is considered to be a kind of important in Orange Producing
Quarantine disease.
The prevention and treatment of citrus bacterial canker disease is mainly taken based on chemical prevention at present, but this can only be reduced to a certain extent
The disease incidence of citrus bacterial canker disease, and with the continuous reuse of chemical agent, citrus processing can be to some chemical drugs
Agent is developed drug resistance, while the chemical agent that part uses will also result in fruit pesticide residue, and to environment, there is also to a certain degree
On pollution.
Feature of the bio-control method because of it safely, effectively, lasting and environmental-friendly, citrus bacterial canker disease prevention and treatment on by
Gradually have become a hot topic of research.A large amount of antagonistic microbe is screened out, and mandarin orange is used in such a way that blade face and fruit spray
The prevention and treatment of tangerine canker.However, microbial inoculum sprays and takes a lot of work laborious, climate is affected, and biological control emphasis is still in " improvement ",
Fail to give full play to effect of the microbial bacterial agent in terms of " prevention ".
Summary of the invention
The present invention proposes the organic fertilizer and its method of administration for preventing and treating citrus bacterial canker disease, and this method is using organic fertilizer as carrier
Microbial bacterial agent is prevented and treated applied to citrus bacterial canker disease, and specifies additive amount of the microbial bacterial agent in organic fertilizer carrier, is had
Have that the lasting period is long, resistance risk is small, application is simple and the advantages of having citrus growth-promoting concurrently.
In order to achieve the above object, the present invention is achieved through the following technical solutions:
For preventing and treating the organic fertilizer of citrus bacterial canker disease, including following raw material components: organic fertilizer carrier, Bacillus subtilis and
Trichoderma harzianum.
Further, organic fertilizer is feces of livestock and poultry, stalk hybrid composting and sufficiently decomposed product, and C/N ratio is 15:1-
25:1 is air-dried to moisture content 30% hereinafter, crushed 10 meshes.
Further, Bacillus subtilis connects bacterium amount not less than 4 × 1011CFU/g in organic fertilizer carrier, is frozen with bacterium solution
The form and organic fertilizer carrier of dry powder mix.
Further, Trichoderma harzianum connects bacterium amount not less than 1 × 1010CFU/g, with bacterium solution freeze-dried powder in organic fertilizer carrier
Form and organic fertilizer carrier mix.
A kind of for preventing and treating the method for administration of the organic fertilizer of citrus bacterial canker disease, winter applies fertilizer, and it is organic to compound microbial bacterial agent
Fertilizer applies 30cm wide, in the deep organic fertilizer ditch of 40cm after mixing with soil according to 3:1, and in surface blinding.
Further, in fertilising, organic fertilizer ditch need to prolong tree crown drip periphery two sides and open straight flute or the open loop around tree crown
Shape ditch.
Compared with prior art, the invention has the following advantages:
1. the effects of microbial bacterial agent of the present invention can be by generating competition, antagonism, bacteriolyze with germ, thus
Inhibit canker germ physiological activity, and by mechanism such as Inducing plant growth resistances, plays protection and treatment double effects, In
Citrus bacterial canker disease and growth promotion the aspect significant effect compared with current other measures is prevented and treated to improve.
2. activity and time-to-live that the organic fertilizer carrier that the present invention uses can effectively improve microbe inoculation.Microorganism
After microbial inoculum colonizes 15d for the first time, living bacteria count declines within 10%.
3. the organic fertilizer carrier that the present invention uses can provide nutrition for tree body, improve tree body rhizospheric environment, enhance tree vigo(u)r,
To generate the common prevention and treatment citrus bacterial canker disease of synergistic effect with microbial bacterial agent.
4. the present invention applies compounding microbial bacterial agent organic fertilizer by the winter to prevent and treat citrus bacterial canker disease, skill is sprayed with current microbial inoculum
Art is compared, from switching to " putting prevention first " " based on improvement ".
Specific embodiment
Below in conjunction with the embodiment of the present invention, technical scheme in the embodiment of the invention is clearly and completely described,
Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based in the present invention
Embodiment, every other embodiment obtained by those of ordinary skill in the art without making creative efforts, all
Belong to the scope of protection of the invention.
Embodiment 1
Fresh cow dung is mixed with wheat stalk according to 3:2, aerobic compost and sufficiently it is decomposed after, air-dry and crushed
Sieve.Its C/N ratio is 18.7, moisture content 28%.
It is inoculated with Bacillus subtilis (Bacillus subtilis) bacterium solution freeze-dried powder, connecing bacterium amount is 5 × 1011CFU/g, is connect
Kind Trichoderma harzianum (Trichoderma harzianum) bacterium solution freeze-dried powder, connecing bacterium amount is 2 × 1010CFU/g.It is uniformly mixed and is made
Compound microbial bacterial agent organic fertilizer.It is 8 × 1010CFU/g, Trichoderma harzianum viable count that Bacillus subtilis viable count is measured after 15d
For 1.6 × 1010CFU/g.
Embodiment 2
Fresh cow dung is mixed with corn stover according to 3:2, aerobic compost and sufficiently it is decomposed after, air-dry and crushed
Sieve.Its C/N ratio is 22.1, moisture content 26%.
It is inoculated with Bacillus subtilis (Bacillus subtilis) bacterium solution freeze-dried powder, connecing bacterium amount is 4 × 1011CFU/g, is connect
Kind Trichoderma harzianum (Trichoderma harzianum) bacterium solution freeze-dried powder, connecing bacterium amount is 1 × 1010CFU/g.It is uniformly mixed and is made
Compound microbial bacterial agent organic fertilizer.It is 7 × 1010CFU/g, Trichoderma harzianum viable count that Bacillus subtilis viable count is measured after 15d
For 8 × 109CFU/g.
Embodiment 3
Fresh chicken manure is mixed with rice straw according to 1:1, aerobic compost and sufficiently it is decomposed after, air-dry and crushed
Sieve.Its C/N ratio is 25.2, moisture content 27%.
It is inoculated with Bacillus subtilis (Bacillus subtilis) bacterium solution freeze-dried powder, connecing bacterium amount is 5 × 1011CFU/g, is connect
Kind Trichoderma harzianum (Trichoderma harzianum) bacterium solution freeze-dried powder, connecing bacterium amount is 1 × 1010CFU/g.It is uniformly mixed and is made
Compound microbial bacterial agent organic fertilizer.It is 7 × 1010CFU/g, Trichoderma harzianum viable count that Bacillus subtilis viable count is measured after 15d
For 6 × 109CFU/g.
The experiment of control in field citrus bacterial canker disease:
In Yichang, Dangyang City carries out the experiment of control in field citrus bacterial canker disease.Test mandarin tree is the Wenzhou for being colonized 13 years
Mandarin orange, planting density are 70 plants/acre.The compounding microbial bacterial agent organic fertilizer that embodiment 1,2,3 obtains disposably is applied as winter top dressing
Enter, dose is 2000kg/ mus, and fertilizing method is to prolong tree crown drip to open the deep annular groove of 30cm wide, 40cm, compounds microbial bacteria
Agent organic fertilizer applies in organic fertilizer ditch after mixing with soil according to 3:1, and in surface blinding.At the same time, it is set in adjacent area
Set the control of conventional fertilizer application.
The canker of the focal investigation citrus spring tip and autumn growth prevents and treats situation, and observes influence of the embodiment to Ci trus growth.
Each embodiment and control respectively 10 plants of citruses of random searching, every plant of tree east, south, west, north, in 5 points sampling, every investigation 2 newly
Whole blades on the tip, record each grade blade and fruit, and calculate disease index and control efficiency.
Disease incidence (the %)=disease number of sheets/investigation total number of sheets × 100
Disease index={ Σ (the morbidity numbers at different levels × disease series)/(investigation sum × highest series) } × 100
Opposite control efficiency (%)=(check plot disease index-embodiment disease index)/control disease index × 100
1 embodiment of table with compare the crop field field efficacy to citrus bacterial canker disease
Note: indicated after disease incidence and disease index difference embodiment the lowercase different with compareing indicate the embodiment with
There are significant difference (p < 0.05) for control
The result shows that embodiment can significantly reduce the disease incidence of citrus bacterial canker disease under field condition, mandarin orange is significantly reduced
Tangerine canker disease index, relative comparison control efficiency is able to maintain 40% or more, and the lasting period is longer.
For art technology, it is clear that the present invention is not limited to the details of above-mentioned exemplary embodiment for this, and in this hair
In the case where bright spirit or essential attributes, the present invention can be realized in other specific forms.Which point therefore, no matter come from
See, the present embodiments are to be considered as illustrative and not restrictive, the scope of the present invention by appended claims without
It is that above description limits, accordingly, it is intended to which all changes for falling in the meaning and scope of the equivalent requirements of the claims are included
In the present invention.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Include an independent technical solution, this narrating mode of specification, it is only for for the sake of clear, those skilled in the art are answered
When considering the specification as a whole, the technical solutions in the embodiments may also be suitably combined to form those skilled in the art
The other embodiment that member is understood that.
Claims (6)
1. the organic fertilizer for preventing and treating citrus bacterial canker disease, it is characterised in that: including following raw material components: organic fertilizer carrier, withered grass
The full bacillus of bud and Trichoderma harzianum.
2. according to claim 1 for preventing and treating the organic fertilizer of citrus bacterial canker disease, it is characterised in that: organic fertilizer is animal dung
Just, stalk hybrid composting and sufficiently decomposed product, C/N ratio are 15:1-25:1, are air-dried to moisture content 30% hereinafter, crushing
Cross 10 meshes.
3. according to claim 1 for preventing and treating the organic fertilizer of citrus bacterial canker disease, it is characterised in that: Bacillus subtilis exists
Bacterium amount is connect not less than 4 × 1011CFU/g in organic fertilizer carrier, is mixed in the form of bacterium solution freeze-dried powder with organic fertilizer carrier.
4. according to claim 1 for preventing and treating the organic fertilizer of citrus bacterial canker disease, it is characterised in that: Trichoderma harzianum is organic
Bacterium amount is connect not less than 1 × 1010CFU/g in fertile carrier, is mixed in the form of bacterium solution freeze-dried powder with organic fertilizer carrier.
5. a kind of for preventing and treating the method for administration of the organic fertilizer of citrus bacterial canker disease, it is characterised in that: winter fertilising compounds microorganism
Microbial inoculum organic fertilizer applies 30cm wide, in the deep organic fertilizer ditch of 40cm after mixing with soil according to 3:1, and in surface blinding.
6. according to claim 5 for preventing and treating the method for administration of the organic fertilizer of citrus bacterial canker disease, it is characterised in that: applying
When fertile, organic fertilizer ditch need to prolong tree crown drip periphery two sides and open straight flute or open annular groove around tree crown.
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| CN201910843333.3A CN110452035A (en) | 2019-09-06 | 2019-09-06 | For preventing and treating the organic fertilizer and its method of administration of citrus bacterial canker disease |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910843333.3A CN110452035A (en) | 2019-09-06 | 2019-09-06 | For preventing and treating the organic fertilizer and its method of administration of citrus bacterial canker disease |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1901797A (en) * | 2004-01-09 | 2007-01-24 | 生物系统公司 | Bioimmune-aggressin composition for suppression of xanthomonad infections in agriculture crops |
| CN102333857A (en) * | 2009-01-27 | 2012-01-25 | 乐斯福公司 | Saccharomyces cerevisiae strains with phytosanitary capabilities |
| CN105110996A (en) * | 2015-09-29 | 2015-12-02 | 黄尤 | Fertilizer special for navel orange and preparation method thereof |
| CN108083920A (en) * | 2017-12-29 | 2018-05-29 | 大工（青岛）新能源材料技术研究院有限公司 | Microbial manure and its preparation method and application |
| CN108191480A (en) * | 2018-03-09 | 2018-06-22 | 易虹 | A kind of biological organic fertilizer and preparation method thereof |
-
2019
- 2019-09-06 CN CN201910843333.3A patent/CN110452035A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1901797A (en) * | 2004-01-09 | 2007-01-24 | 生物系统公司 | Bioimmune-aggressin composition for suppression of xanthomonad infections in agriculture crops |
| CN102333857A (en) * | 2009-01-27 | 2012-01-25 | 乐斯福公司 | Saccharomyces cerevisiae strains with phytosanitary capabilities |
| CN105110996A (en) * | 2015-09-29 | 2015-12-02 | 黄尤 | Fertilizer special for navel orange and preparation method thereof |
| CN108083920A (en) * | 2017-12-29 | 2018-05-29 | 大工（青岛）新能源材料技术研究院有限公司 | Microbial manure and its preparation method and application |
| CN108191480A (en) * | 2018-03-09 | 2018-06-22 | 易虹 | A kind of biological organic fertilizer and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 周俊辉: "《柿 桃 梅 人心果 板栗栽培》", 30 November 2004, 广州：广东科技出版社 * |
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Application publication date: 20191115 |