CN110357704A - A kind of soil remediation microbial inoculum preparation method and applications - Google Patents

A kind of soil remediation microbial inoculum preparation method and applications Download PDF

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Publication number
CN110357704A
CN110357704A CN201910543748.9A CN201910543748A CN110357704A CN 110357704 A CN110357704 A CN 110357704A CN 201910543748 A CN201910543748 A CN 201910543748A CN 110357704 A CN110357704 A CN 110357704A
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China
Prior art keywords
soil remediation
microbial inoculum
raw material
soil
remediation microbial
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Pending
Application number
CN201910543748.9A
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Chinese (zh)
Inventor
袁和玲
袁隆平
田彦超
赵华伟
李梦
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Shenzhen Yuan Da Gaoke Agricultural Development Co Ltd
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Shenzhen Yuan Da Gaoke Agricultural Development Co Ltd
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Priority to CN201910543748.9A priority Critical patent/CN110357704A/en
Publication of CN110357704A publication Critical patent/CN110357704A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C21/00Methods of fertilising, sowing or planting
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00Other inorganic fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Soil Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Inorganic Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Processing Of Solid Wastes (AREA)
  • Fertilizers (AREA)

Abstract

The invention discloses a kind of soil remediation microbial inoculum preparation method and applications, it is related to soil improvement recovery technique field, it is characterized in that, described includes following raw material composition: agricultural crop straw (wheat stalk, corn stover etc.), furfural dregs, N P and K particle, flyash, active powdered carbon and other soil remediation strains, and the soil remediation microbial inoculum preparation method is the following steps are included: raw material selection → raw material cleaning, drying → raw material be crushed → is stirred → strain fermentation → synthesis proportion.A kind of soil remediation microbial inoculum preparation method and applications of the invention, many of thallus is provided with conducive to soil remediation and soil improvement, and antibacterial pest-resistant disease-resistant effect can be played, it can also avoid soil hardening, acidification enhances the gas permeability of soil, and the thallus fermentation of preparation method uses composite fermentation technique, rich in bioactive substances such as exocellular polysaccharides, enables to high crop yield high-quality and guarantee the sustainable efficient farming of soil.

Description

A kind of soil remediation microbial inoculum preparation method and applications
Technical field
The present invention relates to soil remediation microbial inoculum processing technique fields, more particularly to a kind of soil remediation microbial inoculum preparation method And its application.
Background technique
Microbial inoculum, the product being made of one or several kinds the microorganism of specific function and culture medium, additive (carrier), extensively General to be applied to feed addition, organic material composting, soil improvement and repair process, the dirty water decontamination handles etc., microbial inoculum is manured into soil After, wherein the survival rate of thallus and active size by soil variables (edaphon situation, soil water regime, The content of organic matter and pH) and microbial inoculum sole mass influence.
Modern agriculture applied a large amount of industrial chemical fertilizer, cause anthropogenic soil soil fertility to decline, agglomeration is serious, under crop yield Drop, moreover, as industrial pollution, water pollution, atmosphere pollution increasingly sharpen, agricultural cultivating soil while soil fertility declines, Different degrees of pollution is also suffered from, it is therefore desirable to soil remediation microbial inoculum is used, for this purpose, it is proposed that a kind of soil remediation microbial inoculum Preparation method and applications.
Summary of the invention
In order to achieve the above object, the present invention is achieved by the following technical programs: a kind of soil remediation microbial inoculum preparation side Method and its application, including following raw material composition: agricultural crop straw (wheat stalk, corn stover etc.), furfural dregs, N P and K Grain, flyash, active powdered carbon and other soil remediation strains, the soil remediation microbial inoculum preparation method the following steps are included:
(1), raw material is chosen: it is main component that raw material, which should select agricultural crop straw wheat stalk, corn stover etc., and is equipped with chaff Aldehyde slag, N P and K particle, flyash, active powdered carbon and other soil remediation strains.
(2), raw material cleaning, drying: agricultural crop straw wheat stalk, corn stover etc. are cleaned first, then high temperature Drying residual moisture content, while high temperature being made to generate sterilization effect.
(3), raw material is broken: being crushed to agricultural crop straw wheat stalk, corn stover etc., its particle should be made when broken Less than 0.2cm, in closed space be crushed, reduce floating dust wave and the waste of raw material.
(4), be stirred: by the raw materials such as the agricultural crop straw wheat stalk of broken powdering, corn stover and furfural dregs, N P and K particle, flyash and active powdered carbon are proportionally placed in blender and are stirred mixing, so that furfural dregs, N P and K Particle, flyash and active powdered carbon can be uniformly dispersed in powdery agricultural crop straw raw material, by observing its tool when mixing Volume morphing judges whether to be uniformly mixed.
(5), bacillus polymyxa, bacillus licheniformis, bacillus megaterium, bacillus subtilis strain fermentation: are chosen With five kinds of Trichoderma, using Zymolysis Equipment to strain carry out fermenting and producing, finally five kinds of strain special ratios are mixed to get mixed Combined bacteria body, and the mixing thallus is soluble powder, wherein living bacteria count >=2 × 1010cfu/g.
(6), comprehensive proportion: the classes raw material such as agricultural crop straw wheat stalk, corn stover after the completion of being stirred is made It for carrier, is proportionally placed in be stirred in batch mixer with strain raw material after fermentation and mixes, and observe in batch mixer The material in portion mixes situation, until the material mixed-forming inside batch mixer, obtained mixture is soil remediation microbial inoculum.
Preferably, the agricultural crop straw wheat stalk, corn stover etc., furfural dregs, N P and K particle, flyash and work Property powdered carbon mass ratio be 8:5:2:1:1.
Preferably, the bacillus polymyxa, bacillus licheniformis, bacillus megaterium, bacillus subtilis and trichoderma The thallus mixing mass ratio of bacterium is 8:7:10:3:5.
Preferably, it is described be stirred after the completion of agricultural crop straw raw material carrier and thallus mass ratio and 6:1.
Preferably, it includes following application method that the soil remediation microbial inoculum, which is applied,;
(1), base is applied: 500~1000g of soil remediation microbial inoculum is used per acre, dilutes 300-500 times, and can press 1 with organic fertilizer: The ratio mixing of 50~1:100 imposes in furrow, applies rear earthing;
(2), cave is applied: soil remediation microbial inoculum 100g~300g is applied in every cave, dilutes 300-500 times, serious plot can take the circumstances into consideration dosage;
(3), it row replacement or spreads fertilizer over the fields: per acre using soil remediation microbial inoculum 1000g, diluting 300-500 times, and need to keep soil wet Profit, applies rear earthing;
(4), seed dressing, root dipping: add clear water to be adjusted to paste appropriate soil remediation microbial inoculum, with seed mixing, sow, cover after slightly drying Soil.
Soil remediation microbial inoculum preparation method and applications of the present invention have the advantage that
1, raw material agricultural crop straw wheat stalk, corn stover etc. are main component, and are equipped with and are equipped with furfural dregs, N P and K Particle, flyash and active powdered carbon, wherein cost is relatively low for agricultural crop straw wheat stalk, corn stover etc., while furfural dregs Also there is certain natural fertility, and N P and K particle is capable of providing nutrient needed for crop growth in soil, favorably In the fertility for enhancing soil while soil remediation, it should be made when agricultural crop straw wheat stalk, corn stover etc. are broken Grain is less than 0.2cm, and convenient for the natural degradation in later period, the addition of flyash, active carbon improves convenient for improveing to acid soil Soil permeability promotes slightly solubility mineral matter element in soil to become soluble nutriment.
2, bacillus polymyxa can induce plant and generate disease resistance, has apparent promotion growth, improves yield, improves product The effect of matter, bacillus licheniformis are convenient for that body is promoted to generate antibacterial substance, kill pathogenic bacteria, moreover it is possible to generate resistant activity object Matter, and there is unique biology take oxygen mechanism, the growth and breeding of pathogenic bacteria can be inhibited, bacillus megaterium can use next life It produces phosphorus decomposing and consolidates potash fertilizer, bacillus megaterium has effect organic phosphorus in degradation soil well, and Trichoderma can produce a variety of right Plant pathogenic fungi, bacterium and insect have the bioactive substance of antagonism, and can improve the resistance of crops, promote Plant growth and raising agricultural output.
3, the thallus fermentation of preparation method uses composite fermentation technique, can be rich in the bioactive substances such as exocellular polysaccharide Breeding becomes dominant microflora rapidly in soil, forms soil and the benign micro-ecological environment of rhizosphere based on beneficial bacteria, makes to make Object high yield and high quality and the guarantee sustainable efficient farming of soil, being provided with for many of thallus change conducive to soil remediation and soil It is good, and antibacterial pest-resistant disease-resistant effect can be played, additionally it is possible to soil hardening is avoided, is acidified, the gas permeability of soil is enhanced.
Specific embodiment
A kind of soil remediation microbial inoculum preparation method and applications, described includes following raw material composition: agricultural crop straw (wheat Stalk, corn stover etc.), furfural dregs, N P and K particle, flyash, active powdered carbon and other soil remediation strains, the soil Remediation microbial inoculum preparation method the following steps are included:
(1), raw material is chosen: it is main component that raw material, which should select agricultural crop straw wheat stalk, corn stover etc., and is equipped with chaff Aldehyde slag, N P and K particle, flyash, active powdered carbon and other soil remediation strains.
(2), raw material cleaning, drying: agricultural crop straw wheat stalk, corn stover etc. are cleaned first, then high temperature Drying residual moisture content, while high temperature being made to generate sterilization effect.
(3), raw material is broken: being crushed to agricultural crop straw wheat stalk, corn stover etc., its particle should be made when broken Less than 0.2cm, in closed space be crushed, reduce floating dust wave and the waste of raw material.
(4), be stirred: by the raw materials such as the agricultural crop straw wheat stalk of broken powdering, corn stover and furfural dregs, N P and K particle, flyash and active powdered carbon are proportionally placed in blender and are stirred mixing, so that furfural dregs, N P and K Particle, flyash and active powdered carbon can be uniformly dispersed in powdery agricultural crop straw raw material, by observing its tool when mixing Volume morphing judges whether to be uniformly mixed.
(5), bacillus polymyxa, bacillus licheniformis, bacillus megaterium, bacillus subtilis strain fermentation: are chosen With five kinds of Trichoderma, using Zymolysis Equipment to strain carry out fermenting and producing, finally five kinds of strain special ratios are mixed to get mixed Combined bacteria body, and the mixing thallus is soluble powder, wherein living bacteria count >=2 × 1010cfu/g.
(6), comprehensive proportion: the classes raw material such as agricultural crop straw wheat stalk, corn stover after the completion of being stirred is made It for carrier, is proportionally placed in be stirred in batch mixer with strain raw material after fermentation and mixes, and observe in batch mixer The material in portion mixes situation, until the material mixed-forming inside batch mixer, obtained mixture is soil remediation microbial inoculum.
It includes following application method that the soil remediation microbial inoculum, which is applied,;
(1), base is applied: 500~1000g of soil remediation microbial inoculum is used per acre, dilutes 300-500 times, and can press 1 with organic fertilizer: The ratio mixing of 50~1:100 imposes in furrow, applies rear earthing;
(2), cave is applied: soil remediation microbial inoculum 100g~300g is applied in every cave, dilutes 300-500 times, serious plot can take the circumstances into consideration dosage;
(3), it row replacement or spreads fertilizer over the fields: per acre using soil remediation microbial inoculum 1000g, diluting 300-500 times, and need to keep soil wet Profit, applies rear earthing;
(4), seed dressing, root dipping: add clear water to be adjusted to paste appropriate soil remediation microbial inoculum, with seed mixing, sow, cover after slightly drying Soil.
The basic principles, main features and advantages of the invention have been shown and described above.The technical staff of the industry should Understand, the present invention is not limited to the above embodiments, and the above embodiments and description only describe originals of the invention Reason, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes and improvements It all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by appending claims and equivalents circle It is fixed.

Claims (5)

1. a kind of soil remediation microbial inoculum preparation method and applications, which is characterized in that described includes following raw material composition: crops Stalk (wheat stalk, corn stover etc.), furfural dregs, N P and K particle, flyash, active powdered carbon and other soil remediation bacterium Kind, the soil remediation microbial inoculum preparation method the following steps are included:
(1), raw material is chosen: it is main component that raw material, which should select agricultural crop straw (wheat stalk, corn stover etc.), and is equipped with Furfural dregs, N P and K particle, flyash, active powdered carbon and other soil remediation strains;
(2), raw material cleaning, drying: first cleaning agricultural crop straw (wheat stalk, corn stover etc.), and then high temperature dries Dry residual moisture content, while high temperature being made to generate sterilization effect;
(3), raw material is broken: being crushed to agricultural crop straw (wheat stalk, corn stover etc.), its particle should be made small when broken In 0.2cm, in closed space be crushed, reduce floating dust wave and the waste of raw material;
(4), it is stirred: by agricultural crop straw (the wheat stalk, corn stover etc.) raw material and furfural dregs, nitrogen of broken powdering Phosphorus k particle, flyash and active powdered carbon are proportionally placed in blender and are stirred mixing, so that furfural dregs, N P and K Grain, flyash and active powdered carbon can be uniformly dispersed in powdery agricultural crop straw raw material, and when mixing is specific by observing it Form judges whether to be uniformly mixed;
(5), bacillus polymyxa, bacillus licheniformis, bacillus megaterium, bacillus subtilis and wood strain fermentation: are chosen Five kinds of mould, fermenting and producing is carried out to strain using Zymolysis Equipment, five kinds of strain special ratios are finally mixed to get Mixed Microbes Body, and the mixing thallus is soluble powder, wherein living bacteria count >=2 × 1010cfu/g;
(6), comprehensive proportion: using (wheat stalk, corn stover etc.) the class raw material of the agricultural crop straw after the completion of being stirred as Carrier, is proportionally placed in be stirred in batch mixer and mixes with strain raw material after fermentation, and observes inside batch mixer Material mix situation, until batch mixer inside material mixed-forming, obtained mixture is soil remediation microbial inoculum.
2. a kind of soil remediation microbial inoculum preparation method according to claim 1, which is characterized in that including the crops straw Stalk (wheat stalk, corn stover etc.), furfural dregs, N P and K particle, flyash and active powdered carbon mass ratio be 8:5:2:1: 1。
3. a kind of soil remediation microbial inoculum preparation method according to claim 1, it is characterised in that: more viscous gemma bars Bacterium, bacillus licheniformis, bacillus megaterium, bacillus subtilis and Trichoderma thallus mixing mass ratio be 8:7:10:3: 5。
4. a kind of soil remediation microbial inoculum preparation method according to claim 1, it is characterised in that: described to be stirred completion The mass ratio and 6:1 of agricultural crop straw raw material carrier and thallus afterwards.
5. a kind of soil remediation microbial inoculum preparation method and applications according to claim 1-4, it is characterised in that: It includes following application method that the soil remediation microbial inoculum, which is applied,;
(1), base is applied: 500~1000g of soil remediation microbial inoculum is used per acre, dilutes 300-500 times, and can press 1 with organic fertilizer: The ratio mixing of 50~1:100 imposes in furrow, applies rear earthing;
(2), cave is applied: soil remediation microbial inoculum 100g~300g is applied in every cave, dilutes 300-500 times, serious plot can take the circumstances into consideration dosage;
(3), it row replacement or spreads fertilizer over the fields: per acre using soil remediation microbial inoculum 1000g, diluting 300-500 times, and need to keep soil wet Profit, applies rear earthing;
(4), seed dressing, root dipping: add clear water to be adjusted to paste appropriate soil remediation microbial inoculum, with seed mixing, sow, cover after slightly drying Soil.
CN201910543748.9A 2019-06-21 2019-06-21 A kind of soil remediation microbial inoculum preparation method and applications Pending CN110357704A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113368835A (en) * 2021-05-12 2021-09-10 重庆文理学院 Environment repairing material and preparation method and application thereof
CN115044516A (en) * 2022-07-15 2022-09-13 上海山恒生态科技股份有限公司 Soil remediation microbial inoculum and preparation method thereof
CN116656376A (en) * 2023-05-31 2023-08-29 辽宁省微生物科学研究院 Preparation method and application of microbial conditioner

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113368835A (en) * 2021-05-12 2021-09-10 重庆文理学院 Environment repairing material and preparation method and application thereof
CN115044516A (en) * 2022-07-15 2022-09-13 上海山恒生态科技股份有限公司 Soil remediation microbial inoculum and preparation method thereof
CN116656376A (en) * 2023-05-31 2023-08-29 辽宁省微生物科学研究院 Preparation method and application of microbial conditioner
CN116656376B (en) * 2023-05-31 2024-01-05 辽宁省微生物科学研究院 Preparation method and application of microbial conditioner

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Application publication date: 20191022