CN110313619A - A kind of compound peptide and preparation method thereof with liver protecting - Google Patents
A kind of compound peptide and preparation method thereof with liver protecting Download PDFInfo
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- CN110313619A CN110313619A CN201910718592.3A CN201910718592A CN110313619A CN 110313619 A CN110313619 A CN 110313619A CN 201910718592 A CN201910718592 A CN 201910718592A CN 110313619 A CN110313619 A CN 110313619A
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- 230000007246 mechanism Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 235000021239 milk protein Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 229960005181 morphine Drugs 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 230000004792 oxidative damage Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- -1 oxygen radical Chemical class 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000002685 pulmonary effect Effects 0.000 description 1
- 239000003507 refrigerant Substances 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 239000003549 soybean oil Substances 0.000 description 1
- 201000011549 stomach cancer Diseases 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 150000003505 terpenes Chemical class 0.000 description 1
- 235000007586 terpenes Nutrition 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 102000014898 transaminase activity proteins Human genes 0.000 description 1
- 208000025301 tympanitis Diseases 0.000 description 1
- 208000019206 urinary tract infection Diseases 0.000 description 1
- 230000002227 vasoactive effect Effects 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
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- 229930003231 vitamin Natural products 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
- A23L29/37—Sugar alcohols
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The present invention relates to a kind of compound peptides and preparation method thereof with liver protecting, belong to food processing field, content meter by weight, including following components: 5~9 parts of tree peony peptide, 11~17 parts of peanut peptide, 15~23 parts of Semen Maydis polypeptide, 8~13 parts of air bladder polypeptide, 0.5~1 part of dandelion, 1~4 part of fructus lycii, 3~7 parts of Semen Coicis, 1~8 part of xylitol；Preparation method of the invention, it will first be pre-processed after protein dissolution, make protease site exposure, can be improved enzymolysis efficiency, then with multienzyme fractional hydrolysis method, protein is digested, content of peptides in hydrolysate can be increased and reduce free aminoacid content, tree peony peptide, peanut peptide, Semen Maydis polypeptide and air bladder polypeptide are respectively obtained after enzymatic hydrolysis, then other compositions in formula are pulverized and sieved, it is mixed with protein peptides, obtains compound peptide；Gained compound peptide of the invention has effects that liver protecting.
Description
Technical field
The present invention relates to a kind of compound peptides and preparation method thereof with liver protecting, belong to food processing field.
Background technique
Liver is internal most important metabolic organ, but there are some pairs of livers in the Nature and human industry's production process
Dirty virose substance, referred to as " hepatotropic poison ", these poisonous substances in crowd it is universal it is susceptible, incubation period is short, the process of lesion with
The dosage of infection is directly related, necrosis of liver cells, fat deformation, cirrhosis and the liver cancer that liver can be caused different degrees of；Liver
Disease nowadays has become one of most common disease of the mankind, seriously threatens human health；Therefore, seek some natural products
Ingredient can efficiently protect liver to have become the topic of extensive concern.
Early in the 50-60 age, treatment and liver protection measure to hepatitis are in high sugar, high protein, homovitamin and low fat
" three high and one low " principle under one of the main means treated, it is believed that as long as the drug for being conducive to " three high and one low "
There is liver protection, then supplements a large amount of vitamins, inosine, inositol, liver parent pleasure, dissopropylamine ascorbate etc. to patient, pass through facing for many years
Bed observation seem now this therapeutic modality be passively, it is passive, incomplete；After the eighties, with the free radical cause of disease
The foothold of foundation, liver protecting is changed, take energetically " to because of property " protect, effectively eliminate free radical,
The mobility and integrality of cell membrane are protected, liver enzyme and energy synthesis etc. are promoted, liver protecting drug of new generation is just met the tendency of as a result,
And it is raw.
In recent years, the height that the biologically active peptide in food protein enzymolysis product causes domestic and foreign scholars is closed
Note, the especially anti-oxidation peptide with delaying senility function have become the noticeable research direction in current biological active peptide field
One of, such as fish protein polypeptide, egg protein polypeptide, milk protein peptides, soya-bean polypeptides and Peanut Polypeptide；In recent years, and have more
Item is studies have shown that these anti-oxidation peptides all have one to acute liver caused by galactosamine, carbon tetrachloride and alcohol
Fixed protective effect, and prompt the realization of these functions that may depend on its antioxidant activity, some scholars develop work
Property polypeptide can promote the maturing process (cell is made to be grown to serve as mature cell from puerilism) of cell to be continuously replenished by
Damage liver cell；It can repair and be damaged incomplete mitochondria in liver cell, restore (ATP) energy circulation；It can also repair molten in liver cell
Enzyme body removes intracorporal toxin.Therefore, how a variety of biologically active peptide compoundings with liver protection function to be arrived together, and
It realizes maximum bioactivity value, becomes the research emphasis of present each researcher.
Summary of the invention
It is an object of the invention to overcome the problems of the prior art, providing a kind of has effects that the compound of liver protecting
Peptide.
It is another object of the present invention to provide a kind of preparation methods of compound peptide with liver protecting.
The purpose of the present invention is achieved by the following technical programs:
A kind of compound peptide with liver protecting, content meter, including following components by weight: tree peony peptide 5~9
Part, 11~17 parts of peanut peptide, 15~23 parts of Semen Maydis polypeptide, 8~13 parts of air bladder polypeptide, 0.5~1 part of dandelion, 1~4 part of fructus lycii,
3~7 parts of Semen Coicis, 1~8 part of xylitol.
Contain IGF-I growth factor in tree peony peptide, there is hypoglycemic effect, tree peony peptide has vasoactive Angiotensin Converting Enzyme to turn
Change the site exposure of enzyme, can with blood pressure lowering, by adjust hormone, class morphine sample activity, the transport for improving minerals and
It absorbs, adjustable taste, improves human qi and blood running.
Peanut peptide rich in nutrition content, amino acid classes are complete, it is necessary to which amino acid content is up to 38.29%, has complete
Independent mechanism of absorption and it is better than free amino acid, the peptide fragment formed after different aminoacids permutation and combination has the function of different work
Property.The more hypotensive activity for peanut peptide, antioxidation activity and bacteriostasis antibiosis, immunological regulation etc. are studied at present
Functional activity, some researches show that the accumulation that peanut peptide can obviously to total bile acid in anti-oxidative damage rat blood serum inhibits paddy third
The raising of transaminase and alkaline phosphatase activities improves superoxide dismutase from liver, glutathione peroxidase and mistake
Hydrogen oxide enzyme activity, and can reduce the lipofuscin content in rat liver, effectively mitigate the histopathology form of rat liver
Variation is learned, shows that the Liver Damage in Rats that peanut peptide induces D- galactolipin has certain protective effect.
Semen Maydis polypeptide is the product after zein enzymatic hydrolysis, these Semen Maydis polypeptides are made of special acid, is such as rich in paddy
Propylhomoserin, proline, leucine and alanine, these four amino acid contents account for about 60%, are rich in hydrophobic amino acid, Zhan Suoyou ammonia
The 50%~60% of base acid residue, these features assign Semen Maydis polypeptide multiple biological function, improve liver venereal disease symptom, reduce blood
Clearing gallbladder sterol, anti peroxidation of lipid supplement protein, antifatigue, inhibit hypertensin conversion enzyme activity, corn peptide contains about
13% alanine, is 3 times of soybean peptide, 5 times of wheat peptide, and the speed that alanine can make body absorb ethyl alcohol slows down, and
It can promote alcohol metabolism.
Air bladder polypeptide is the mixture for the short peptide chain that fish maw protein generates after hydrolysis, because it is with inoxidizability, drop blood
Pressure reduces the different physiological roles such as cholesterol and is paid more and more attention, and research shows ﹒ O2 -The induction of He ﹒ OH isoreactivity oxygen radical
Oxidative damage have always been considered as being one of the reason of causing aging, cellular damage and canceration, air bladder peptide is rich in hydrogen donor, has
The ability of Hydrogen Proton is provided, the radical reduction with high oxidation can be made, to terminate free radical chain reactions, is played clear
Remove or inhibit the purpose of free radical.
Dandelion alias grandmother fourth, Pu Gongcao etc.；Dandelion is as a kind of traditional Chinese medicine, biological active matter rich in
Matter, including sterol, alkaloid, terpene, glycoprotein, oligosaccharide etc.；Dandelion has clearing heat and detoxicating, dispersing swelling and dissipating binds, dampness removing, diuresis
Treating stranguria, Hepatoprotective cholagogue and other effects, modern pharmacological studies have shown that it is with anti-oxidant, antifatigue, antibacterial and a variety of works such as anti-inflammatory
With, and it is cheap, toxic side effect is small, be widely used in furunculosis swelling and pain, acute mastitis, ophthalmodynia hot eyes, pulmonary abscess, puckery pain of heat gonorrhea, urgency
The treatment of the inflammations diseases such as property mazoitis, flu.
Fructus lycii is the dry mature fruit of China's matrimony vine of solanaceae plant, is traditional rare traditional Chinese medicine, is health ministry
A batch is classified as one of integration of drinking and medicinal herbs kind, sweet in flavor, mild-natured, has the effect of nourishing liver and kidney, benefiting shrewd head and adjusts immunity of organism
The functions such as power, anti-aging, anti-fatty liver, the polysaccharides in fructus lycii is one of liver-protective main active of fructus lycii,
It is main that by improving oxidation resistance, the expression of inhibition inflammation, lipid peroxidation and cromoci YP2E1 gene plays a role,
There is significant protective effect to alcohol, heavy metal and drug-induced chemical damage.
The nutritive value of Semen Coicis is very high, is known as " king of world gramineae plant ".Semen Coicis can be used as grain and eat, taste and
Rice is similar, and absorption easy to digest, cooks congee, makees Tang Junke.Summer and autumn and wax gourd cooking can not only go with rice or bread edible, but also can clear heat benefit
It is wet.Since Semen Coicis is full of nutrition, for weakness due to chronic disease, reconvalescent, old man, puerpera, children are relatively good medicines after being ill
With food, can often take.No matter effect all more mitigates, and slightly cold without injuring one's stomach, beneficial spleen is not for nourishing or for curing the disease
It grows greasy.The composition coixenolide that Semen Coicis is cured the disease not only has tonic effect, but also is a kind of anticancer agent, can inhibit Emhorn abdomen
Water cancer cell can be used for gastric cancer and cervix cancer.Semen Coicis alcohol contained in the root of Semen Coicis, except the work with above-mentioned coixenolide
With outer, there are also decompression, diuresis, antipyretic and ascarifuge effects, suitable for hypertension, lithangiuria, urinary tract infections, roundworm disease
Deng.The leaf of Semen Coicis can decoct water and make tea-drinking, taste faint scent, drink can be with diuresis.
Xylitol is a kind of multi-sugar alcohol substance, is white crystalline particle or powder, has refrigerant sweet taste, sugariness
As sucrose, thermal stability is good and Maillard reaction will not occur, and nutritive value with higher is in polyalcohols product
The sweetener of most sweet tea and the normal intermediate of human body carbohydate metabolism.Xylitol can be used as the nutritional agents and sugar of diabetic
The regulator of metabolism has good preventing caries function, can prevent pulmonary infection, tympanitis and osteoporosis.
Multiple protein peptide synergistic function, compensates for single peptide chain, has a single function, the unconspicuous disadvantage of curative effect, simultaneously
The dandelion and fructus lycii with liver protection function, invigorating spleen and reinforcing stomach are compounded, the adlay and sweetener xylitol of inducing diuresis for removing edema are enriched
Nutritional ingredient improves mouthfeel, plays the role of heightening the effect of a treatment.
Preferably, content meter by weight, the compound peptide includes following components: 6~8 parts of tree peony peptide, peanut peptide 13~
15 parts, 17~20 parts of Semen Maydis polypeptide, 10~12 parts of air bladder polypeptide, 0.7~0.9 part of dandelion, 2~3 parts of fructus lycii, Semen Coicis 4~6
Part, 3~6 parts of xylitol.
It is furthermore preferred that content meter by weight, the compound peptide includes following components: 7.32 parts of tree peony peptide, peanut peptide
14.11 parts, 18.94 parts of Semen Maydis polypeptide, 11.5 parts of air bladder polypeptide, 0.81 part of dandelion, 2.51 parts of fructus lycii, 5.17 parts of Semen Coicis, wood
4.32 parts of sugar alcohol.
Have effects that the preparation method of the compound peptide of liver protecting described in a kind of, comprising the following steps:
S1. tree peony protein 24~28 part are taken；
S2. deionized water dissolving is used, solid-liquid ratio is 1:8~12, then is pre-processed；
S3. liquid obtained by step S2 is heated to 40~50 DEG C, adjusts pH to 7.0~8.0 with NaOH, carbohydrase 1~2 is added
Part, 0.5~1.5 part of amylase, magnetic agitation hydrolyzes 4~6h, and NaOH is continuously added in enzymolysis process, maintains pH value of solution, enzyme
After having solved, high-temperature inactivation；
S4. 40~50 DEG C are cooled to, pH to 8.0~9.0 is adjusted with NaOH, is added 0.5~1 part of cellulase, withered grass bar
1~2 part of mycoproteinase, 2~3h of magnetic agitation, high-temperature inactivation；
S5. pH to 4.0~5.0 is adjusted with HCl, 4000~5000r/min is centrifuged 10~20min, dry；
S6. tree peony peptide is obtained；
S7. 28~33 parts of peanut protein are taken, S2~S5 is repeated, obtains peanut peptide；
S8. 24~28 parts of zein are taken, S2~S5 is repeated, obtains Semen Maydis polypeptide；
S9. 20~25 parts of fish maw protein are taken, S2~S5 is repeated, obtains air bladder polypeptide；
S10. dandelion, fructus lycii, Semen Coicis, xylitol are crushed, sieving, with tree peony peptide, flower prepared by step S6~S9
Raw peptide, Semen Maydis polypeptide and the mixing of air bladder polypeptide, stir evenly, obtain compound peptide.
Preferably, pretreatment described in the step S2 can be in Short Time Heating, microwave treatment, ultrasonic wave, super-pressure
One kind.
Pretreatment can destroy protein conformation, and protein structure is made to become loose, make protease site exposure, Ke Yiti
High enzymolysis efficiency；Since action site of the different enzymes on substrate is different, using multienzyme fractional hydrolysis, hydrolysate can be increased
Middle content of peptides simultaneously reduces amino acid content.
Preferably, drying mode described in the step S5 is vacuum freeze drying, spray drying, one in heated-air drying
Kind.
Preferably, the high-temperature inactivation condition is persistently to inactivate 5~15min at a temperature of 90~100 DEG C.
Preferably, 80~100 meshes are crossed in the step S10.
The present invention has following technical effect that compared with prior art
A kind of compound peptide and preparation method thereof with liver protecting, using multienzyme fractional hydrolysis, due to different enzymes
Action site on substrate is different, can increase content of peptides in hydrolysate and reduce amino acid content；To individually have
Polypeptide, tree peony peptide, peanut peptide, Semen Maydis polypeptide, the air bladder polypeptide of liver protection function combine use, have brought it about association
With the effect of synergy, hepatic injury, chemical damage, irritability hepatic injury, alcoholic hepatic injury etc. can be caused various galactolipin
Hepatic injury has certain protective effect, and compounding is the same as bioactive substance dandelion and the integration of drinking and medicinal herbs for having liver protection function
Substance fructus lycii and invigorating spleen and reinforcing stomach, the adlay and sweetener xylitol of inducing diuresis for removing edema, have additional nutrients ingredient, improves mouthfeel, enhancing
The effect of liver protecting.
Specific embodiment
It to make the object, technical solutions and advantages of the present invention clearer, combined with specific embodiments below will with comparative example
Technical solution of the present invention is described in detail.Obviously, described embodiments are only a part of the embodiments of the present invention,
Instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not making creative labor
Obtained all other embodiment under the premise of dynamic belongs to the range that the present invention is protected.
Except specified otherwise, equipment used in the present embodiment, comparative example and experimental example is routine experiment equipment, used
Reagent be commercially available.
Embodiment 1
A kind of compound peptide with liver protecting, content meter, including following components by weight: tree peony peptide 7.32
Part, 14.11 parts of peanut peptide, 18.94 parts of Semen Maydis polypeptide, 11.5 parts of air bladder polypeptide, 0.81 part of dandelion, 2.51 parts of fructus lycii, Semen Coicis
5.17 parts, 4.32 parts of xylitol.
A kind of preparation method of the compound peptide with liver protecting, comprising the following steps:
S1. tree peony protein 26 part is taken；
S2. deionized water dissolving, solid-liquid ratio 1:10 are used, then is pre-processed；
S3. liquid obtained by step S2 is heated to 45 DEG C, adjusts pH to 7.5 with NaOH, is added 1.5 parts of carbohydrase, amylase
0.8 part, magnetic agitation hydrolyzes 5h, and NaOH is continuously added in enzymolysis process, maintains pH value of solution, after having digested, high-temperature inactivation；
S4. 45 DEG C are cooled to, pH to 8.5 is adjusted with NaOH, is added 0.7 part of cellulase, subtilopeptidase A 1.5
Part, magnetic agitation 2.5h, high-temperature inactivation；
S5. pH to 4.5,4500r/min is adjusted with HCl be centrifuged 15min, vacuum freeze drying；
S6. tree peony peptide is obtained；
S7. 30 parts of peanut protein are taken, S2~S5 is repeated, obtains peanut peptide；
S8. 27 parts of zein are taken, S2~S5 is repeated, obtains Semen Maydis polypeptide；
S9. 20 parts of fish maw protein are taken, S2~S5 is repeated, obtains air bladder polypeptide；
S10. dandelion, fructus lycii, Semen Coicis, xylitol are crushed, sieving, with tree peony peptide, flower prepared by step S6~S9
Raw peptide, Semen Maydis polypeptide and the mixing of air bladder polypeptide, stir evenly, obtain compound peptide.
Embodiment 2
A kind of compound peptide with liver protecting, content meter, including following components by weight: 5 parts of tree peony peptide, flower
Raw 11 parts of peptide, 15 parts of Semen Maydis polypeptide, 8 parts of air bladder polypeptide, 0.5 part of dandelion, 1 part of fructus lycii, 3 parts of Semen Coicis, 1 part of xylitol.
A kind of preparation method of the compound peptide with liver protecting, comprising the following steps:
S1. tree peony protein 26 part is taken；
S2. deionized water dissolving, solid-liquid ratio 1:10 are used, then is pre-processed；
S3. liquid obtained by step S2 is heated to 45 DEG C, adjusts pH to 7.5 with NaOH, is added 1.5 parts of carbohydrase, amylase
0.8 part, magnetic agitation hydrolyzes 5h, and NaOH is continuously added in enzymolysis process, maintains pH value of solution, after having digested, high-temperature inactivation；
S4. 45 DEG C are cooled to, pH to 8.5 is adjusted with NaOH, is added 0.7 part of cellulase, subtilopeptidase A 1.5
Part, magnetic agitation 2.5h, high-temperature inactivation；
S5. pH to 4.5,4500r/min is adjusted with HCl be centrifuged 15min, spray drying；
S6. tree peony peptide is obtained；
S7. 30 parts of peanut protein are taken, S2~S5 is repeated, obtains peanut peptide；
S8. 27 parts of zein are taken, S2~S5 is repeated, obtains Semen Maydis polypeptide；
S9. 23 parts of fish maw protein are taken, S2~S5 is repeated, obtains air bladder polypeptide；
S10. dandelion, fructus lycii, Semen Coicis, xylitol are crushed, sieving, with tree peony peptide, flower prepared by step S6~S9
Raw peptide, Semen Maydis polypeptide and the mixing of air bladder polypeptide, stir evenly, obtain compound peptide.
Embodiment 3
A kind of compound peptide with liver protecting, content meter, including following components by weight: 9 parts of tree peony peptide, flower
Raw 17 parts of peptide, 23 parts of Semen Maydis polypeptide, 13 parts of air bladder polypeptide, 1 part of dandelion, 4 parts of fructus lycii, 7 parts of Semen Coicis, 8 parts of xylitol.
A kind of preparation method of the compound peptide with liver protecting, comprising the following steps:
S1. tree peony protein 26 part is taken；
S2. deionized water dissolving, solid-liquid ratio 1:10 are used, then is pre-processed；
S3. liquid obtained by step S2 is heated to 45 DEG C, adjusts pH to 7.5 with NaOH, is added 1.5 parts of carbohydrase, amylase
0.8 part, magnetic agitation hydrolyzes 5h, and NaOH is continuously added in enzymolysis process, maintains pH value of solution, after having digested, high-temperature inactivation；
S4. 45 DEG C are cooled to, pH to 8.5 is adjusted with NaOH, is added 0.7 part of cellulase, subtilopeptidase A 1.5
Part, magnetic agitation 2.5h, high-temperature inactivation；
S5. pH to 4.5,4500r/min is adjusted with HCl be centrifuged 15min, heated-air drying；
S6. tree peony peptide is obtained；
S7. 30 parts of peanut protein are taken, S2~S5 is repeated, obtains peanut peptide；
S8. 27 parts of zein are taken, S2~S5 is repeated, obtains Semen Maydis polypeptide；
S9. 25 parts of fish maw protein are taken, S2~S5 is repeated, obtains air bladder polypeptide；
S10. dandelion, fructus lycii, Semen Coicis, xylitol are crushed, sieving, with tree peony peptide, flower prepared by step S6~S9
Raw peptide, Semen Maydis polypeptide and the mixing of air bladder polypeptide, stir evenly, obtain compound peptide.
Comparative example 1
A kind of compound peptide with liver protecting, content meter, including following components by weight: tree peony peptide 3.33
Part, 9.21 parts of peanut peptide, 13.14 parts of Semen Maydis polypeptide, 6.44 parts of air bladder polypeptide, 0.74 part of dandelion, 2.17 parts of fructus lycii, Semen Coicis
4.53 parts, 3.86 parts of xylitol.
A kind of preparation method of the compound peptide with liver protecting, comprising the following steps:
S1. tree peony protein 26 part is taken；
S2. deionized water dissolving, solid-liquid ratio 1:10 are used, then is pre-processed；
S3. liquid obtained by step S2 is heated to 45 DEG C, adjusts pH to 7.5 with NaOH, is added 1.5 parts of carbohydrase, amylase
0.8 part, magnetic agitation hydrolyzes 5h, and NaOH is continuously added in enzymolysis process, maintains pH value of solution, after having digested, high-temperature inactivation；
S4. 45 DEG C are cooled to, pH to 8.5 is adjusted with NaOH, is added 0.7 part of cellulase, subtilopeptidase A 1.5
Part, magnetic agitation 2.5h, high-temperature inactivation；
S5. pH to 4.5,4500r/min is adjusted with HCl be centrifuged 15min, vacuum freeze drying；
S6. tree peony peptide is obtained；
S7. 30 parts of peanut protein are taken, S2~S5 is repeated, obtains peanut peptide；
S8. 27 parts of zein are taken, S2~S5 is repeated, obtains Semen Maydis polypeptide；
S9. 20 parts of fish maw protein are taken, S2~S5 is repeated, obtains air bladder polypeptide；
S10. dandelion, fructus lycii, Semen Coicis, xylitol are crushed, sieving, with tree peony peptide, flower prepared by step S6~S9
Raw peptide, Semen Maydis polypeptide and the mixing of air bladder polypeptide, stir evenly, obtain compound peptide.
Compared with Example 1, tree peony peptide, peanut peptide, Semen Maydis polypeptide, air bladder content of peptides contain this comparative example lower than the present invention
Amount.
Comparative example 2
A kind of compound peptide with liver protecting, content meter, including following components by weight: tree peony peptide 11.31
Part, 19.24 parts of peanut peptide, 25.43 parts of Semen Maydis polypeptide, 14.58 parts of air bladder polypeptide, 0.81 part of dandelion, 2.51 parts of fructus lycii, Semen Coicis
5.17 parts, 4.32 parts of xylitol.
A kind of preparation method of the compound peptide with liver protecting, comprising the following steps:
S1. tree peony protein 26 part is taken；
S2. deionized water dissolving, solid-liquid ratio 1:10 are used, then is pre-processed；
S3. liquid obtained by step S2 is heated to 45 DEG C, adjusts pH to 7.5 with NaOH, is added 1.5 parts of carbohydrase, amylase
0.8 part, magnetic agitation hydrolyzes 5h, and NaOH is continuously added in enzymolysis process, maintains pH value of solution, after having digested, high-temperature inactivation；
S4. 45 DEG C are cooled to, pH to 8.5 is adjusted with NaOH, is added 0.7 part of cellulase, subtilopeptidase A 1.5
Part, magnetic agitation 2.5h, high-temperature inactivation；
S5. pH to 4.5,4500r/min is adjusted with HCl be centrifuged 15min, vacuum freeze drying；
S6. tree peony peptide is obtained；
S7. 30 parts of peanut protein are taken, S2~S5 is repeated, obtains peanut peptide；
S8. 27 parts of zein are taken, S2~S5 is repeated, obtains Semen Maydis polypeptide；
S9. 20 parts of fish maw protein are taken, S2~S5 is repeated, obtains air bladder polypeptide；
S10. dandelion, fructus lycii, Semen Coicis, xylitol are crushed, sieving, with tree peony peptide, flower prepared by step S6~S9
Raw peptide, Semen Maydis polypeptide and the mixing of air bladder polypeptide, stir evenly, obtain compound peptide.
Compared with Example 1, tree peony peptide, peanut peptide, Semen Maydis polypeptide, air bladder content of peptides contain this comparative example higher than the present invention
Amount.
Comparative example 3
A kind of compound peptide with liver protecting, content meter, including following components by weight: tree peony peptide 7.32
Part, 14.11 parts of peanut peptide, 18.94 parts of Semen Maydis polypeptide, 11.5 parts of air bladder polypeptide, 0.33 part of dandelion, 0.56 part of fructus lycii, Semen Coicis
5.17 parts, 4.32 parts of xylitol.
A kind of preparation method of the compound peptide with liver protecting, comprising the following steps:
S1. tree peony protein 26 part is taken；
S2. deionized water dissolving, solid-liquid ratio 1:10 are used, then is pre-processed；
S3. liquid obtained by step S2 is heated to 45 DEG C, adjusts pH to 7.5 with NaOH, is added 1.5 parts of carbohydrase, amylase
0.8 part, magnetic agitation hydrolyzes 5h, and NaOH is continuously added in enzymolysis process, maintains pH value of solution, after having digested, high-temperature inactivation；
S4. 45 DEG C are cooled to, pH to 8.5 is adjusted with NaOH, is added 0.7 part of cellulase, subtilopeptidase A 1.5
Part, magnetic agitation 2.5h, high-temperature inactivation；
S5. pH to 4.5,4500r/min is adjusted with HCl be centrifuged 15min, vacuum freeze drying；
S6. tree peony peptide is obtained；
S7. 30 parts of peanut protein are taken, S2~S5 is repeated, obtains peanut peptide；
S8. 27 parts of zein are taken, S2~S5 is repeated, obtains Semen Maydis polypeptide；
S9. 20 parts of fish maw protein are taken, S2~S5 is repeated, obtains air bladder polypeptide；
S10. dandelion, fructus lycii, Semen Coicis, xylitol are crushed, sieving, with tree peony peptide, flower prepared by step S6~S9
Raw peptide, Semen Maydis polypeptide and the mixing of air bladder polypeptide, stir evenly, obtain compound peptide.
Compared with Example 1, dandelion and fructus lycii content are lower than content of the present invention to this comparative example.
Comparative example 4
A kind of compound peptide with liver protecting, content meter, including following components by weight: tree peony peptide 7.32
Part, 14.11 parts of peanut peptide, 18.94 parts of Semen Maydis polypeptide, 11.5 parts of air bladder polypeptide, 1.34 parts of dandelion, 5.54 parts of fructus lycii, Semen Coicis
5.17 parts, 4.32 parts of xylitol.
A kind of preparation method of the compound peptide with liver protecting, comprising the following steps:
S1. tree peony protein 26 part is taken；
S2. deionized water dissolving, solid-liquid ratio 1:10 are used, then is pre-processed；
S3. liquid obtained by step S2 is heated to 45 DEG C, adjusts pH to 7.5 with NaOH, is added 1.5 parts of carbohydrase, amylase
0.8 part, magnetic agitation hydrolyzes 5h, and NaOH is continuously added in enzymolysis process, maintains pH value of solution, after having digested, high-temperature inactivation；
S4. 45 DEG C are cooled to, pH to 8.5 is adjusted with NaOH, is added 0.7 part of cellulase, subtilopeptidase A 1.5
Part, magnetic agitation 2.5h, high-temperature inactivation；
S5. pH to 4.5,4500r/min is adjusted with HCl be centrifuged 15min, vacuum freeze drying；
S6. tree peony peptide is obtained；
S7. 30 parts of peanut protein are taken, S2~S5 is repeated, obtains peanut peptide；
S8. 27 parts of zein are taken, S2~S5 is repeated, obtains Semen Maydis polypeptide；
S9. 20 parts of fish maw protein are taken, S2~S5 is repeated, obtains air bladder polypeptide；
S10. dandelion, fructus lycii, Semen Coicis, xylitol are crushed, sieving, with tree peony peptide, flower prepared by step S6~S9
Raw peptide, Semen Maydis polypeptide and the mixing of air bladder polypeptide, stir evenly, obtain compound peptide.
Compared with Example 1, dandelion and fructus lycii content are higher than content of the present invention to this comparative example.
Comparative example 5
A kind of compound peptide with liver protecting, content meter, including following components by weight: tree peony peptide 7.32
Part, 14.11 parts of peanut peptide, 18.94 parts of Semen Maydis polypeptide, 11.5 parts of air bladder polypeptide, 0.81 part of dandelion, 2.51 parts of fructus lycii, Semen Coicis
5.17 parts, 4.32 parts of xylitol.
A kind of preparation method of the compound peptide with liver protecting, comprising the following steps:
S1. tree peony protein 26 part is taken；
S2. deionized water dissolving, solid-liquid ratio 1:10 are used, then is pre-processed；
S3. liquid obtained by step S2 is heated to 70 DEG C, adjusts pH to 7.5 with NaOH, is added 1.5 parts of carbohydrase, amylase
0.8 part, magnetic agitation hydrolyzes 5h, and NaOH is continuously added in enzymolysis process, maintains pH value of solution, after having digested, high-temperature inactivation；
S4. 70 DEG C are cooled to, pH to 8.5 is adjusted with NaOH, is added 0.7 part of cellulase, subtilopeptidase A 1.5
Part, magnetic agitation 2.5h, high-temperature inactivation；
S5. pH to 4.5,4500r/min is adjusted with HCl be centrifuged 15min, vacuum freeze drying；
S6. tree peony peptide is obtained；
S7. 30 parts of peanut protein are taken, S2~S5 is repeated, obtains peanut peptide；
S8. 27 parts of zein are taken, S2~S5 is repeated, obtains Semen Maydis polypeptide；
S9. 20 parts of fish maw protein are taken, S2~S5 is repeated, obtains air bladder polypeptide；
S10. dandelion, fructus lycii, Semen Coicis, xylitol are crushed, sieving, with tree peony peptide, flower prepared by step S6~S9
Raw peptide, Semen Maydis polypeptide and the mixing of air bladder polypeptide, stir evenly, obtain compound peptide.
Compared with Example 1, step S2 and step S3 reaction temperature are higher than temperature of the present invention to the present invention.
Comparative example 6
A kind of compound peptide with liver protecting, content meter, including following components by weight: tree peony peptide 7.32
Part, 14.11 parts of peanut peptide, 18.94 parts of Semen Maydis polypeptide, 11.5 parts of air bladder polypeptide, 0.81 part of dandelion, 2.51 parts of fructus lycii, Semen Coicis
5.17 parts, 4.32 parts of xylitol.
A kind of preparation method of the compound peptide with liver protecting, comprising the following steps:
S1. tree peony protein 26 part is taken；
S2. deionized water dissolving, solid-liquid ratio 1:10 are used, then is pre-processed；
S3. liquid obtained by step S2 is heated to 30 DEG C, adjusts pH to 7.5 with NaOH, is added 1.5 parts of carbohydrase, amylase
0.8 part, magnetic agitation hydrolyzes 5h, and NaOH is continuously added in enzymolysis process, maintains pH value of solution, after having digested, high-temperature inactivation；
S4. 30 DEG C are cooled to, pH to 8.5 is adjusted with NaOH, is added 0.7 part of cellulase, subtilopeptidase A 1.5
Part, magnetic agitation 2.5h, high-temperature inactivation；
S5. pH to 4.5,4500r/min is adjusted with HCl be centrifuged 15min, vacuum freeze drying；
S6. tree peony peptide is obtained；
S7. 30 parts of peanut protein are taken, S2~S5 is repeated, obtains peanut peptide；
S8. 27 parts of zein are taken, S2~S5 is repeated, obtains Semen Maydis polypeptide；
S9. 20 parts of fish maw protein are taken, S2~S5 is repeated, obtains air bladder polypeptide；
S10. dandelion, fructus lycii, Semen Coicis, xylitol are crushed, sieving, with tree peony peptide, flower prepared by step S6~S9
Raw peptide, Semen Maydis polypeptide and the mixing of air bladder polypeptide, stir evenly, obtain compound peptide.
Compared with Example 1, step S2 and step S3 reaction temperature are lower than temperature of the present invention to the present invention.
Experimental example 1
Mouse is randomly divided into 11 groups, and every group 10, half male and half female；Laboratory room temperature adaptable fed 3d, into experiment periods；
The daily stomach-filling physiological saline 20mL/kg of blank control group, model group, 1~6 gained of experimental group stomach-filling Examples 1 to 3 and comparative example
Compound peptide, given low 50.5mL/kg, continuous gavage 30d, model group and experimental group are primary after last dose fasting 16h
1%CCl is given in property stomach-filling480mg/kg bw, blank control group stomach-filling edible soybean oil.
Glutamic-pyruvic transaminase (ALT), glutamic-oxalacetic transaminease (AST), lactic dehydrogenase (LDH) vigor inspection in serum: fasting is for 24 hours
Afterwards, orbital vein takes blood, and 3000r/min is centrifuged 15min and separates serum, and interior automatic clinical chemistry analyzer checks in serum for 24 hours
ALT, AST, LDH vigor.
All data are all made ofIt indicates, group difference is judged using one-way analysis of variance, p < 0.05 is considered being examined
The difference of survey has statistical significance.
1 mice serum ALT, AST, LDT measurement result of table
Note*P < 0.05,**P < 0.01 is compared with blank group.
Malonaldehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) in liver organization
Viability examination: the disconnected neck of mouse is put to death, and liver is taken to weigh, and physiological saline homogenate, centrifuging and taking supernatant is added, and MDA vigor uses TBA
Method measurement, is carried out by assay kit operating instruction；SOD vigor use colorimetric method for determining, by assay kit operating instruction into
Row；GSH-Px vigor uses colorimetric method for determining, carries out by assay kit operating instruction.
All data are all made ofIt indicates, group difference is judged using one-way analysis of variance, p < 0.05 is considered being examined
The difference of survey has statistical significance.
2 mouse liver tissue MDA, SOD, GSH-Px index determining result of table
Note*P < 0.05,**P < 0.01 is compared with blank group.
CCl4CCl is generated after the activation of hepatomicrosome cytochrome p 4503, CCl3Phospholipid molecule on liver plasma membrane can be attacked, is caused
Lipid peroxidation, or covalent bond occurs with hepatomicrosome lipid and protein, damage the complete of liver plasma membrane structure and function
Property, it penetrates into lyoenzyme AST, ALT, LDH in endochylema in blood, related enzyme activity is caused to reduce, energy production is reduced, and is influenced
Cellular respiration causes the change of cell sample and necrosis.
Tables 1 and 2 the experimental results showed that, Examples 1 to 3 can inhibit CCl4It causes in acute liver model
ALT, AST, LDH are increased, and reduce MDA content in liver, SOD and GSH-Px activity in liver are improved, to CCl4Caused hepatic injury
There is the first protective effect；Comparative example 2 and 4, the increase of Contents of Main Components will not be such that liver-protective function and effect are increasing；
Comparative example 1 and 3, the reduction of Contents of Main Components, comparative example 5 and 6, temperature is raised and lowered, make to ALT, AST, LDH and MDA,
The function and effect of SOD, GSH-Px weaken, and liver-protective function and effect weaken.
The present invention disclose involved in such as "include", "comprise", " having " etc. word be open vocabulary, refer to
" including but not limited to ", it and can be used interchangeably with it.Vocabulary "or" and "and" used herein above refer to vocabulary "and/or", and can
It is used interchangeably with it, unless it is not such that context, which is explicitly indicated,.Vocabulary " such as " used herein above refers to phrase " such as but not
It is limited to ", and can be used interchangeably with it.
In addition, as used herein, the "or" instruction separation used in the enumerating of the item started with "at least one"
It enumerates, such as enumerating for " at least one of A, B or C " means A or B or C or AB or AC or BC or ABC (i.e. A and B and C).
In addition, wording " exemplary " does not mean that the example of description is preferred or more preferable than other examples.
The above description of disclosed aspect is provided so that any person skilled in the art can make or use this
It is open.Various modifications in terms of these are readily apparent to those skilled in the art, and are defined herein
General Principle can be applied to other aspect without departing from the scope of the present disclosure.Therefore, the present invention discloses schematic diagram and is limited
To the aspect being shown here, but according to principle disclosed herein and the consistent widest range of novel feature.
Above description is had been presented for for purposes of illustration and description.In addition, this description is not intended to disclose the present invention
Embodiment be restricted to form disclosed herein.Although already discussed above multiple exemplary aspects and embodiment, ability
Its certain modifications, modification, change, addition and sub-portfolio will be recognized in field technique personnel.
Claims (8)
1. a kind of compound peptide with liver protecting, which is characterized in that content meter by weight, including following components: male
5~9 parts of red peptide, 11~17 parts of peanut peptide, 15~23 parts of Semen Maydis polypeptide, 8~13 parts of air bladder polypeptide, 0.5~1 part of dandelion, Chinese holly
1~4 part of Qi, 3~7 parts of Semen Coicis, 1~8 part of xylitol.
2. having effects that the compound peptide of liver protecting according to claim 1, which is characterized in that content meter by weight, packet
Include following components: 6~8 parts of tree peony peptide, 13~15 parts of peanut peptide, 17~20 parts of Semen Maydis polypeptide, 10~12 parts of air bladder polypeptide, Pu Gong
0.7~0.9 part of English, 2~3 parts of fructus lycii, 4~6 parts of Semen Coicis, 3~6 parts of xylitol.
3. having effects that the compound peptide of liver protecting according to claim 1, which is characterized in that content meter by weight, packet
Include following components: 7.32 parts of tree peony peptide, 14.11 parts of peanut peptide, 18.94 parts of Semen Maydis polypeptide, 11.5 parts of air bladder polypeptide, dandelion
0.81 part, 2.51 parts of fructus lycii, 5.17 parts of Semen Coicis, 4.32 parts of xylitol.
4. having effects that the preparation method of the compound peptide of liver protecting described in a kind of any one of claims 1 to 3, feature exists
In, comprising the following steps:
S1. tree peony protein 24~28 part are taken；
S2. deionized water dissolving is used, solid-liquid ratio is 1:8~12, then is pre-processed；
S3. liquid obtained by step S2 is heated to 40~50 DEG C, adjusts pH to 7.0~8.0 with NaOH, carbohydrase 1~2 is added
Part, 0.5~1.5 part of amylase, magnetic agitation hydrolyzes 4~6h, and NaOH is continuously added in enzymolysis process, maintains pH value of solution, enzyme
After having solved, high-temperature inactivation；
S4. 40~50 DEG C are cooled to, pH to 8.0~9.0 is adjusted with NaOH, is added 0.5~1 part of cellulase, hay bacillus egg
1~2 part, 2~3h of magnetic agitation of white enzyme, high-temperature inactivation；
S5. pH to 4.0~5.0 is adjusted with HCl, 4000~5000r/min is centrifuged 10~20min, dry；
S6. tree peony peptide is obtained；
S7. 28~33 parts of peanut protein are taken, S2~S5 is repeated, obtains peanut peptide；
S8. 24~28 parts of zein are taken, S2~S5 is repeated, obtains Semen Maydis polypeptide；
S9. 20~25 parts of fish maw protein are taken, S2~S5 is repeated, obtains air bladder polypeptide；
S10. by dandelion, fructus lycii, Semen Coicis, xylitol crush, sieving, with tree peony peptide, peanut peptide prepared by step S6~S9,
Semen Maydis polypeptide and the mixing of air bladder polypeptide, stir evenly, obtain compound peptide.
5. having effects that the preparation method of the compound peptide of liver protecting according to claim 4, which is characterized in that the step
Pretreatment can be one of Short Time Heating, microwave treatment, ultrasonic wave, super-pressure described in S2.
6. having effects that the preparation method of the compound peptide of liver protecting according to claim 4, which is characterized in that the step
Drying mode described in S5 is one of vacuum freeze drying, spray drying, heated-air drying.
7. having effects that the preparation method of the compound peptide of liver protecting according to claim 4, which is characterized in that the high temperature
Inactivation condition is persistently to inactivate 5~15min at a temperature of 90~100 DEG C.
8. having effects that the preparation method of the compound peptide of liver protecting according to claim 4, which is characterized in that the step
80~100 meshes are crossed in S10.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113142589A (en) * | 2021-02-25 | 2021-07-23 | 广东维尔嘉康生命科技有限公司 | Liver-protecting hexapeptide composition and preparation method thereof |
| CN113679051A (en) * | 2021-08-24 | 2021-11-23 | 广州丹荟生物科技有限公司 | Peony polypeptide powder, solid beverage and application thereof |
| CN115226862A (en) * | 2022-06-23 | 2022-10-25 | 北京宝贝优鲜科技有限公司 | Fish double-protein peptide and preparation method and composition thereof |
-
2019
- 2019-08-05 CN CN201910718592.3A patent/CN110313619A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113142589A (en) * | 2021-02-25 | 2021-07-23 | 广东维尔嘉康生命科技有限公司 | Liver-protecting hexapeptide composition and preparation method thereof |
| CN113679051A (en) * | 2021-08-24 | 2021-11-23 | 广州丹荟生物科技有限公司 | Peony polypeptide powder, solid beverage and application thereof |
| CN115226862A (en) * | 2022-06-23 | 2022-10-25 | 北京宝贝优鲜科技有限公司 | Fish double-protein peptide and preparation method and composition thereof |
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