CN110305902A - 一种在工具细胞中激活hSyn启动子的方法及其应用 - Google Patents

一种在工具细胞中激活hSyn启动子的方法及其应用 Download PDF

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CN110305902A
CN110305902A CN201910629189.3A CN201910629189A CN110305902A CN 110305902 A CN110305902 A CN 110305902A CN 201910629189 A CN201910629189 A CN 201910629189A CN 110305902 A CN110305902 A CN 110305902A
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杨佳丽
杨兴林
马佩敏
杨蕊菊
潘讴东
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Heyuan Biotechnology (shanghai) Co Ltd
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Abstract

本发明涉及基因工程领域，具体提供了一种在工具细胞中激活hSyn启动子的方法及其应用。所述在工具细胞中激活hSyn启动子的方法，采用CRISPR‑Cas9 SAM系统。本发明利用CRIPSR‑Cas9 SAM系统方法，在常见工具细胞中，激活神经元特异性启动子hSyn的活性，用于验证特异启动子在工具细胞中的表达，为检测新型神经组织特异性启动子的病毒表达载体提供体外系统，使其在体内使用提供实验数据参考。此发明不仅在神经生物学研究方面具有非常重要的意义，在利用进行神经系统疾病基因靶向治疗方面也极具应用价值。

Description

一种在工具细胞中激活hSyn启动子的方法及其应用
技术领域
本发明涉及基因工程领域，具体提供了一种在工具细胞中激活hSyn启动子的方法及其应用。
背景技术
启动子是基因的重要组成部分，主要功能是控制基因表达(转录)的起始时间和表达的程度。由于染色体不同折叠程度，转录因子不同表达水平等因素，启动子开放程度和招募的转录因子不同，从而使得大部分基因在不同类型细胞中和时间上表达水平有所差异，呈现出时间特异性和组织特异性表达。hSyn(human Synapsin I)启动子是人SYN1基因的启动子，SYN1基因表达产生Synapsin I蛋白，是特异表达在神经元内的蛋白，因此一般选择hSyn启动子作为神经元的特异性启动子。
CRISPR-Cas9 SAM(synergistic activation mediator)系统是采用切割活性缺失的dCas9蛋白融合VP64转录激活蛋白，加上融合MS2的sgRNA，招募激活辅助蛋白p65和HSF1，实现对目标基因的激活。
腺相关病毒(Adeno-Associated Viral Vector，AAV)属于微小病毒科(parvovirus)，为无包膜的单链线状DNA病毒，具有宿主范围广、安全性高、免疫原性低、表达稳定和物理性质稳定等优点，已被广泛地应用于基础研究和临床试验中，并且腺相关病毒载体已成为世界上最常用的基因治疗载体之一。然而，腺相关病毒体内表达周期长，作用效率不好判断，因此需要在进行体内实验前先进行体外基本作用效率的检测。常用工具细胞可以用于广谱性启动子的检测，但一些特异性启动子由于不表达则无法进行相关检测，因此急需发明一种新的通用的有效检测特异性启动子激活的工具细胞系统。
发明内容
本发明基于现有的CRIPSR-Cas9 SAM系统，构建特异针对hSyn启动子的gRNA，并将dCas9-VP64融合表达质粒、sgRNA(hSyn)质粒和激活辅助蛋白p65-HSF1表达质粒共同导入工具细胞，并将此系统用于检测hSyn启动荧光蛋白的表达来评价激活效率。此方法为检测hSyn特异性启动子的病毒表达载体提供体外系统，使其在体内使用提供实验数据参考，不仅在神经生物学研究方面具有非常重要的意义，在利用进行神经系统疾病基因靶向治疗方面也极具应用价值。
具体的，本发明的技术方案如下：
本发明一方面公开了一种在工具细胞中激活hSyn启动子的方法，采用CRISPR-Cas9 SAM系统。
优选的，上述方法包括：设计合成sgRNA，所述sgRNA为sgRNA1、sgRNA2或sgRNA3，其核苷酸序列分别如SEQ ID NO:1、SEQ ID NO:2和SEQ ID NO:3所示。所述sgRNA特异性的针对hSyn启动子设计并合成。
术语“sgRNA”是指向导RNA(guide RNA，gRNA)，在RNA编辑的过程中引导尿苷残基插入或缺失到动质体(kinetoplastid)中，属于一种小型非编码RNA，可与pre-mRNA配对。gRNA编辑RNA分子，长度大约60-80个核苷酸，由单独的基因转录。
应当理解，与所述SEQ ID NO:1、SEQ ID NO:2和SEQ ID NO:3所示的核苷酸序列具有90％以上的同源性且功能性相同的核苷酸序列均在本发明的保护范围之内。
术语“核苷酸序列”是指核苷酸序列，是指DNA或RNA中碱基的排列顺序。这意味着DNA中A，T，G，C的排列循序，或者mRNA中A，U，G，C的排列循序，也包括rRNA，tRNA中碱基的排列顺序。
更优选的，上述方法包括：
S1：合成权利要求2所述的sgRNA；
S2：将含荧光蛋白标记的慢病毒质粒导入工具细胞中得到工具细胞过表达稳定株；
S3：将dCas9-VP64融合表达质粒、sgRNA质粒和激活辅助蛋白p65-HSF1表达质粒共同导入工具细胞过表达稳定株中，观察荧光。
应该理解，本发明不限于上述步骤，还可以包含其他的步骤，例如在步骤S1之前、步骤S1和S2之间、步骤S2和S3之间、步骤S3之后，还包含其他额外的步骤，而不超出本发明的保护范围。
较佳的，将sgRNA导入空载载体pLenti-U6-gRNA-MS2-EF1a-dCas9-VP64-T2A-BSD中得到sgRNA质粒。在本发明的一具体实施例中，将上述特异性针对hSyn启动子序列的sgRNA1序列、sgRNA2序列和sgRNA3序列分别连入空载载体pLenti-U6-gRNA-MS2-EF1a-dCas9-VP64-T2A-BSD(核酸序列如SEQ ID NO:4所示)得到相应的sgRNA目的载体pLenti-U6-hSyn-gRNA1-MS2-EF1a-dCas9-VP64-T2A-BSD(核酸序列如SEQ ID NO:5所示)、pLenti-U6-hSyn-gRNA2-MS2-EF1a-dCas9-VP64-T2A-BSD(核酸序列如SEQ ID NO:6所示)和pLenti-U6-hSyn-gRNA3-MS2-EF1a-dCas9-VP64-T2A-BSD(核酸序列如SEQ ID NO:7所示)；
术语“空载载体”是指在基因克隆的时一般我们用质粒、病毒等载体将我们感兴趣的目的基因导入宿主细胞内部，那么没有与目的基因链接的这些载体就称之为空载载体。
优选的，所述S2包括步骤S21：构建含荧光蛋白标记的慢病毒质粒。
优选的，所述S21包括以下步骤：
S211：将hSyn-mNeonGreen核苷酸序列连入空载载体pLenti-CMV-EGFP-3FLAG-PGK-Puro中即得到含荧光蛋白标记的慢病毒质粒。应当理解，本发明S211中的载体并不局限空载载体pLenti-CMV-EGFP-3FLAG-PGK-Puro，本领域技术人员根据需要能够选择任何合适的载体来完成本发明，并且在本发明的保护范围之内。
在本发明的一具体实施例中，将hSyn-mNeonGreen核苷酸序列连入空载载体pLenti-CMV-EGFP-3FLAG-PGK-Puro，核苷酸序列如SEQ ID NO:8，得到目的质粒pLenti-hSyn-mNeonGreen-PGK-puro，核苷酸序列如SEQ ID NO:9所示。mNeonGreen是来自文昌鱼(头索)的单体绿色荧光蛋白，经证实，当在哺乳动物细胞表达时它能执行大量靶蛋白的融合标签功能。mNeonGreen在哺乳动物细胞中表达时融合标签执行。不感光的mNeonGreen是蓝绿色荧光蛋白的受体，在活体或固定细胞中作为一种荧光蛋白标签提供单色、超分辨率成像图。
应当理解，本发明中的荧光蛋白并不局限于mNeonGreen蛋白，本领域技术人员根据需要能够选择任何合适的荧光蛋白来完成本发明，并且在本发明的保护范围之内。
在本发明的一具体实施例中，将pLenti-hSyn-mNeonGreen-PGK-puro感染293T，筛选得到hSyn-mNeonGreen的293T过表达稳定株；
将dCas9-VP64融合表达质粒、pLenti-U6-hSyn-gRNA-MS2-EF1a-dCas9-VP64-T2A-BSD、和pLenti-CMV-MCP-P65-HSF1-2A-Hygro共同感染293T过表达稳定株，观察荧光；
在本发明的一具体实施例中，将慢病毒pLenti-hSyn-mNeonGreen-PGK-puro感染NIH/3T3细胞，感染48h后用2ug/ml的嘌呤霉素(puromycin,puro)处理细胞，筛选得到稳定表达hSyn-mNeonGreen的NIH/3T3细胞株。
优选的，所述工具细胞为293T细胞或NIH/3T3细胞。
应当理解，本发明中的工具细胞并不局限于293T细胞或NIH/3T3细胞，本领域技术人员根据需要能够选择任何合适的工具细胞来完成本发明，并且在本发明的保护范围之内。
本发明第二个方面公开了上述的方法在基因工程领域中的应用。
本发明第三个方面首次公开了CRISPR-Cas9 SAM系统在工具细胞中激活hSyn启动子的应用。
不同于传统的将CRISPR-Cas9 SAM系统用于内源基因的表达，本发明开创性的将CRISPR-Cas9 SAM系统用于体外检测新型神经组织特异性启动子的表达，不仅在神经生物学研究方面具有非常重要的意义，在利用进行神经系统疾病基因靶向治疗方面也极具应用价值。进一步的，该神经组织特异性启动子为hSyn启动子。
本发明第四个方面公开了一种用于激活hSyn启动子的sgRNA，所述sgRNA为靶向hSyn启动子sgRNA。通过发明人的创新设计和归纳总结，再进行大量的试验进行比较筛选，从多个sgRNA中挑选出三条性能优越的sgRNA，即sgRNA1、sgRNA2和sgRNA3，其核苷酸序列分别如SEQ ID NO：1、SEQ ID NO：2和SEQ ID NO：3所示。
本发明第五个方面公开了一种试剂盒，所述试剂盒包括上述的sgRNA。该试剂盒可用于在工具细胞中激活hSyn启动子。
在符合本领域常识的基础上，上述各优选条件，可任意组合，而不超出本发明的构思与保护范围。
本发明相对于现有技术具有如下的显著优点及效果：
本发明利用CRIPSR-Cas9 SAM系统，在常见工具细胞(例如293T细胞和NIH/3T3细胞)中，激活神经元特异性启动子hSyn的活性，用于验证特异启动子在工具细胞中的表达，为检测新型神经组织特异性启动子的病毒表达载体提供体外系统，使其在体内使用提供实验数据参考。此发明不仅在神经生物学研究方面具有非常重要的意义，在利用进行神经系统疾病基因靶向治疗方面也极具应用价值。
附图说明
图1为本发明实施例提供的pLenti-U6-gRNA-MS2-EF1a-dCas9-VP64-T2A-BSD载体结构示意图；
图2为本发明实施例提供的pLenti-U6-hSyn-gRNA-MS2-EF1a-dCas9-VP64-T2A-BSD载体结构示意图；
图3为本发明实施例提供的pLenti-CMV-EGFP-3FLAG-PGK-Puro载体结构示意图；
图4为本发明实施例提供的pLenti-hSyn-mNeonGreen-PGK-puro载体结构示意图；
图5为本发明实施例提供的pLenti-CMV-MCS-Wpre载体结构示意图；
图6为本发明实施例提供的pLenti-CMV-MCP-P65-HSF1-2A-Hygro载体结构示意图；
图7为本发明实施例提供的人胚肾细胞(293T)荧光表达图；
图8为本发明实施例提供的小鼠胚胎成纤维细胞(NIH/3T3)荧光表达图。
具体实施方式
下面结合附图和实施例对本发明的技术方案进行详细描述，但并不因此将本发明限制在所述的实施例范围之中。
下列实施例中未注明具体条件的实验方法，按照常规方法和条件，或按照商品说明书选择。本发明所用试剂和原料均市售可得。
实施例1含靶向hSyn启动子sgRNA的慢病毒表达载体构建
本实施例公开了一种含靶向hSyn启动子sgRNA的慢病毒表达载体构建方法，包括以下步骤：
(1)hSyn sgRNA寡核苷酸合成；设计并合成特异靶向hSyn启动子序列的3条sgRNAs，sgRNA1序列如SEQ ID NO:1所示，sgRNA2序列如SEQ ID NO:2所示，sgRNA3序列如SEQ ID NO:3所示；
其中，hSyn sgRNA序列表如下面表1所示。
表1 hSyn sgRNA序列表
hSyn sgRNA1 GCGCTGCCTCAGTCTGCGGTGGG SEQ ID NO:1
hSyn sgRNA2 CCTCAGTCTGCGGTGGGCAGCGG SEQ ID NO:2
hSyn sgRNA3 GGGCGCGACCATCTGCGCTGCGG SEQ ID NO:3
(2)将上述引物对用退火buffer溶解成20uM的浓度，于95℃水浴锅中孵育5min，打开水浴锅盖子慢慢自然冷却至室温；
(3)用BsmB I酶切pLenti-U6-gRNA-MS2-EF1a-dCas9-VP64-T2A-BSD质粒(结构如图1所示)，55℃中酶切4h，得到324bp和9kb的片段，使用GENRY胶回收试剂盒回收9kb的载体；
将(2)中退火产物与(3)中酶切回收的载体片段连接得到目的质粒。将连接后的质粒转化至感受态细胞DH5α中，均匀涂至LB固体培养基平板中，置于37℃培养箱中培养12-16小时，单个菌落即可出现；
(4)挑取单个菌落扩大培养并质粒小提；
(5)测序鉴定质粒构建成功，并命名为pLenti-U6-hSyn-gRNA-MS2-EF1a-dCas9-VP64-T2A-BSD(结构如图2所示)。
实施例2特异性启动子hSyn表达载体的构建
本实施例公开了一种特异性启动子hSyn表达载体的构建方法，包括以下步骤：
(1)合成带有酶切位点及上下游同源臂的hSyn-mNeonGreen核苷酸序列；
(2)将质粒pLenti-CMV-EGFP-3FLAG-PGK-Puro(结构如图3所示)用Mlu I和Xba I进行酶切。50μL酶切反应体系含pLenti-CMV-EGFP-3FLAG-PGK-Puro 10ug、10×Cutsmart(NEB)5μL、Mlu I和Xba I各1μL，用水补足至50μL，37℃酶切1h后，进行1％琼脂糖凝胶电泳，紫外灯下用刀片将大片段切下并进行回收纯化；
(3)将(2)步得到的纯化的酶切产物和(1)步的hSyn-mNeonGreen核苷酸序列进行无缝克隆。50μL反应体系分别含(2)酶切产物102.2ng，(1)合成序列14.3ng，1μL酶，25μLbuffer，用水补足至50μL，50℃反应15min后置于冰上进行转化涂板；
(4)挑取单菌落，进行质粒小提及测序鉴定。经测序，表达载体pLenti-hSyn-mNeonGreen-PGK-puro(结构如图4所示)构建成功。
实施例3 P65-HSF1表达载体的构建
本实施例公开了一种P65-HSF1表达载体的构建方法，包括以下步骤：
(1)合成带有酶切位点及上下游同源臂的MCP-P65-HSF1-2A-Hygro序列；
(2)将质粒pLenti-CMV-MCS-Wpre(结构如图5所示)用Sal I和Nhe I进行酶切。50μL酶切反应体系含pLenti-CMV-MCS-Wpre 10ug、10×Cutsmart(NEB)5μL、Sal I和Nhe I各1μL，用水补足至50μL，37℃酶切1h后，进行1％琼脂糖凝胶电泳，紫外灯下用刀片将大片段切下并进行回收纯化；
(3)将(2)步得到的纯化的酶切产物和(1)步的MCP-P65-HSF1-2A-Hygro核苷酸序列进行无缝克隆。50μL反应体系分别含(2)酶切产物102.2ng，(1)合成序列14.3ng，1μL酶，25μL buffer，用水补足至50μL，50℃反应15min后置于冰上进行转化涂板；
(4)挑取单菌落，进行质粒小提及测序鉴定。经测序，表达载体pLenti-CMV-MCP-P65-HSF1-2A-Hygro(结构如图6所示)构建成功。
实施例4检测在293T细胞中hSyn启动子sgRNA对hSyn激活
本实施例公开了一种检测在293T细胞中hSyn启动子sgRNA对hSyn是否激活的方法，其中实验组包括以下步骤：
(1)Cripsr-cas9 SAM系统相关质粒包装病毒
利用慢病毒三质粒包装系统，将构建好的pLenti-U6-hSyn-gRNA-MS2-EF1a-dCas9-VP64-T2A-BSD、pLenti-hSyn-mNeonGreen-PGK-puro和pLenti-CMV-MCP-P65-HSF1-2A-Hygro分别包装慢病毒；
(2)构建hSyn-mNeonGreen稳定表达细胞株
将慢病毒pLenti-hSyn-mNeonGreen-PGK-puro感染293T细胞，感染48h后用2ug/ml的嘌呤霉素(puromycin,puro)处理细胞，筛选得到稳定表达hSyn-mNeonGreen的293T细胞株；
(3)将分别含实施例1中所公开的3条不同hSyn gRNA的pLenti-U6-hSyn-gRNA-MS2-EF1a-dCas9-VP64-T2A-BSD慢病毒，分别与pLenti-CMV-MCP-P65-HSF1-2A-Hygro慢病毒(图6)共同感染hSyn-mNeonGreen的293T稳定细胞株；
(4)感染48h后，用荧光显微镜观察细胞荧光表达情况。
本实施例还设置有对照组，对照组与实验组的唯一区别在于：未感染含hSynSgRNA的病毒。结果如图7所示，从图中可以看到，与对照组相比，感染含hSyn SgRNA的病毒的实验组荧光明显增强，说明我们的方法可以使得hSyn能在293T细胞中启动mNeonGreen的表达。
实施例5检测在NIH/3T3细胞中hSyn启动子sgRNA对hSyn激活
本实施例公开了一种检测在NIH/3T3细胞中hSyn启动子sgRNA对hSyn是否激活的方法，其中实验组包括以下步骤：
(1)构建hSyn-mNeonGreen稳定表达细胞株
将慢病毒pLenti-hSyn-mNeonGreen-PGK-puro感染NIH/3T3细胞，感染48h后用2ug/ml的嘌呤霉素(puromycin,puro)处理细胞，筛选得到稳定表达hSyn-mNeonGreen的NIH/3T3细胞株；
(2)将分别含实施例1中所公开的3条不同hSyn gRNA的pLenti-U6-hSyn-gRNA-MS2-EF1a-dCas9-VP64-T2A-BSD慢病毒，分别与pLenti-CMV-MCP-P65-HSF1-2A-Hygro慢病毒(图6)共同感染hSyn-mNeonGreen的NIH/3T3细胞株；
(3)感染48h后，用荧光显微镜观察细胞荧光表达情况。
本实施例还设置有对照组，对照组与实验组的唯一区别在于：未感染含hSynSgRNA的病毒。结果如图8所示，从图中可以看到，与对照组相比，感染含hSyn SgRNA的病毒的实验组荧光明显增强，说明我们的方法可以使得hSyn能在NIH/3T3细胞(图8)中启动mNeonGreen的表达。
上述实施例为本发明较佳的实施方式，但本发明的实施方式并不受上述实施例的限制，其他的任何未背离本发明的精神实质与原理下所作的改变、修饰、替代、组合、简化，均应为等效的置换方式，都包含在本发明的保护范围之内。
序列表
<110> 和元生物技术(上海)股份有限公司
<120> 一种在工具细胞中激活hSyn启动子的方法及其应用
<160> 11
<170> SIPOSequenceListing 1.0
<210> 1
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1
gcgctgcctc agtctgcggt ggg 23
<210> 2
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 2
cctcagtctg cggtgggcag cgg 23
<210> 3
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 3
gggcgcgacc atctgcgctg cgg 23
<210> 4
<211> 13806
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 4
gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 60
atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 120
gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 180
tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 240
attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 300
atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 360
acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 420
tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 480
tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 540
attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 600
tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 660
ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 720
accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 780
gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 840
ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 900
aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 960
tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1020
gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1080
ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1140
ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1200
ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1260
aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1320
tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1380
caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1440
aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1500
aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1560
agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1620
gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1680
ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1740
acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1800
ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1860
ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1920
tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1980
aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2040
aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2100
aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2160
acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2220
agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2280
tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2340
gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2400
gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2460
tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2520
agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2580
agatccagtt tggttaatta gctagcgagg gcctatttcc catgattcct tcatatttgc 2640
atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga 2700
tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa 2760
aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct 2820
tggctttata tatcttgtgg aaaggacgaa acaccggaga cgggaatgca gtttaaggtt 2880
tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt 2940
gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa 3000
gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc 3060
accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac 3120
cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata ataccgtctc 3180
tgttttagag ctaggccaac atgaggatca cccatgtctg cagggcctag caagttaaaa 3240
taaggctagt ccgttatcaa cttggccaac atgaggatca cccatgtctg cagggccaag 3300
tggcaccgag tcggtgcttt ttttggatcc taggtcttga aaggagtggg aattggctcc 3360
ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg 3420
gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc 3480
gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc 3540
gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggcgta cggccaccat 3600
gaaaaggccg gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagg acaagaagta 3660
cagcatcggc ctggccatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta 3720
caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa 3780
gaacctgatc ggagccctgc tgttcgacag cggcgaaaca gccgaggcca cccggctgaa 3840
gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat 3900
cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt 3960
cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca tcgtggacga 4020
ggtggcctac cacgagaagt accccaccat ctaccacctg agaaagaaac tggtggacag 4080
caccgacaag gccgacctgc ggctgatcta tctggccctg gcccacatga tcaagttccg 4140
gggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt 4200
catccagctg gtgcagacct acaaccagct gttcgaggaa aaccccatca acgccagcgg 4260
cgtggacgcc aaggccatcc tgtctgccag actgagcaag agcagacggc tggaaaatct 4320
gatcgcccag ctgcccggcg agaagaagaa tggcctgttc ggcaacctga ttgccctgag 4380
cctgggcctg acccccaact tcaagagcaa cttcgacctg gccgaggatg ccaaactgca 4440
gctgagcaag gacacctacg acgacgacct ggacaacctg ctggcccaga tcggcgacca 4500
gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc tgagcgacat 4560
cctgagagtg aacaccgaga tcaccaaggc ccccctgagc gcctctatga tcaagagata 4620
cgacgagcac caccaggacc tgaccctgct gaaagctctc gtgcggcagc agctgcctga 4680
gaagtacaaa gagattttct tcgaccagag caagaacggc tacgccggct acattgacgg 4740
cggagccagc caggaagagt tctacaagtt catcaagccc atcctggaaa agatggacgg 4800
caccgaggaa ctgctcgtga agctgaacag agaggacctg ctgcggaagc agcggacctt 4860
cgacaacggc agcatccccc accagatcca cctgggagag ctgcacgcca ttctgcggcg 4920
gcaggaagat ttttacccat tcctgaagga caaccgggaa aagatcgaga agatcctgac 4980
cttccgcatc ccctactacg tgggccctct ggccagggga aacagcagat tcgcctggat 5040
gaccagaaag agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg tggacaaggg 5100
cgcttccgcc cagagcttca tcgagcggat gaccaacttc gataagaacc tgcccaacga 5160
gaaggtgctg cccaagcaca gcctgctgta cgagtacttc accgtgtata acgagctgac 5220
caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa 5280
aaaggccatc gtggacctgc tgttcaagac caaccggaaa gtgaccgtga agcagctgaa 5340
agaggactac ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga 5400
tcggttcaac gcctccctgg gcacatacca cgatctgctg aaaattatca aggacaagga 5460
cttcctggac aatgaggaaa acgaggacat tctggaagat atcgtgctga ccctgacact 5520
gtttgaggac agagagatga tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga 5580
caaagtgatg aagcagctga agcggcggag atacaccggc tggggcaggc tgagccggaa 5640
gctgatcaac ggcatccggg acaagcagtc cggcaagaca atcctggatt tcctgaagtc 5700
cgacggcttc gccaacagaa acttcatgca gctgatccac gacgacagcc tgacctttaa 5760
agaggacatc cagaaagccc aggtgtccgg ccagggcgat agcctgcacg agcacattgc 5820
caatctggcc ggcagccccg ccattaagaa gggcatcctg cagacagtga aggtggtgga 5880
cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg aaatggccag 5940
agagaaccag accacccaga agggacagaa gaacagccgc gagagaatga agcggatcga 6000
agagggcatc aaagagctgg gcagccagat cctgaaagaa caccccgtgg aaaacaccca 6060
gctgcagaac gagaagctgt acctgtacta cctgcagaat gggcgggata tgtacgtgga 6120
ccaggaactg gacatcaacc ggctgtccga ctacgatgtg gaccacatcg tgcctcagag 6180
ctttctgaag gacgactcca tcgacaacaa ggtgctgacc agaagcgaca aggcccgggg 6240
caagagcgac aacgtgccct ccgaagaggt cgtgaagaag atgaagaact actggcggca 6300
gctgctgaac gccaagctga ttacccagag aaagttcgac aatctgacca aggccgagag 6360
aggcggcctg agcgaactgg ataaggccgg cttcatcaag agacagctgg tggaaacccg 6420
gcagatcaca aagcacgtgg cacagatcct ggactcccgg atgaacacta agtacgacga 6480
gaatgacaag ctgatccggg aagtgaaagt gatcaccctg aagtccaagc tggtgtccga 6540
tttccggaag gatttccagt tttacaaagt gcgcgagatc aacaactacc accacgccca 6600
cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga 6660
aagcgagttc gtgtacggcg actacaaggt gtacgacgtg cggaagatga tcgccaagag 6720
cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc tacagcaaca tcatgaactt 6780
tttcaagacc gagattaccc tggccaacgg cgagatccgg aagcggcctc tgatcgagac 6840
aaacggcgaa accggggaga tcgtgtggga taagggccgg gattttgcca ccgtgcggaa 6900
agtgctgagc atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt 6960
cagcaaagag tctatcctgc ccaagaggaa cagcgataag ctgatcgcca gaaagaagga 7020
ctgggaccct aagaagtacg gcggcttcga cagccccacc gtggcctatt ctgtgctggt 7080
ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag agctgctggg 7140
gatcaccatc atggaaagaa gcagcttcga gaagaatccc atcgactttc tggaagccaa 7200
gggctacaaa gaagtgaaaa aggacctgat catcaagctg cctaagtact ccctgttcga 7260
gctggaaaac ggccggaaga gaatgctggc ctctgccggc gaactgcaga agggaaacga 7320
actggccctg ccctccaaat atgtgaactt cctgtacctg gccagccact atgagaagct 7380
gaagggctcc cccgaggata atgagcagaa acagctgttt gtggaacagc acaagcacta 7440
cctggacgag atcatcgagc agatcagcga gttctccaag agagtgatcc tggccgacgc 7500
taatctggac aaagtgctgt ccgcctacaa caagcaccgg gataagccca tcagagagca 7560
ggccgagaat atcatccacc tgtttaccct gaccaatctg ggagcccctg ccgccttcaa 7620
gtactttgac accaccatcg accggaagag gtacaccagc accaaagagg tgctggacgc 7680
caccctgatc caccagagca tcaccggcct gtacgagaca cggatcgacc tgtctcagct 7740
gggaggcgac agcgctggag gaggtggaag cggaggagga ggaagcggag gaggaggtag 7800
cggacctaag aaaaagagga aggtggcggc cgctggatcc ggacgggctg acgcattgga 7860
cgattttgat ctggatatgc tgggaagtga cgccctcgat gattttgacc ttgacatgct 7920
tggttcggat gcccttgatg actttgacct cgacatgctc ggcagtgacg cccttgatga 7980
tttcgacctg gacatgctga ttaactgtac aggcagtgga gagggcagag gaagtctgct 8040
aacatgcggt gacgtcgagg agaatcctgg cccaatggcc aagcctttgt ctcaagaaga 8100
atccaccctc attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta 8160
cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata 8220
tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc 8280
agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc 8340
ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa 8400
ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt 8460
gtgggagggc taagaattcg atatcaagct tatcggtaat caacctctgg attacaaaat 8520
ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc 8580
tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt 8640
gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg 8700
cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg 8760
tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc 8820
cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt 8880
gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct 8940
gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg 9000
cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg 9060
gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca 9120
tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc 9180
acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac 9240
ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct 9300
aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta 9360
cttccctgat tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg 9420
atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga 9480
gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt 9540
attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc 9600
ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 9660
agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 9720
ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 9780
aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc 9840
agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca 9900
ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta 9960
ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc 10020
atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta 10080
gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 10140
gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 10200
cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 10260
ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 10320
cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 10380
tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 10440
cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 10500
aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc 10560
cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 10620
gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 10680
gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 10740
cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 10800
ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 10860
caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat 10920
taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg 10980
gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag 11040
ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg 11100
gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac 11160
accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc 11220
gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg 11280
tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag 11340
gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg 11400
ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg 11460
ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc 11520
aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 11580
tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg 11640
gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 11700
aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 11760
acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 11820
cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 11880
tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 11940
tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 12000
gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 12060
aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 12120
ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 12180
gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 12240
ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 12300
ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 12360
cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 12420
attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 12480
ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 12540
aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 12600
gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 12660
tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 12720
ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 12780
taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 12840
atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 12900
actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 12960
cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 13020
agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 13080
gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 13140
gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 13200
gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 13260
gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 13320
cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 13380
ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 13440
accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 13500
aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 13560
aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 13620
caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 13680
ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 13740
gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 13800
cctgac 13806
<210> 5
<211> 13500
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 5
gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 60
atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 120
gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 180
tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 240
attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 300
atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 360
acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 420
tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 480
tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 540
attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 600
tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 660
ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 720
accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 780
gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 840
ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 900
aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 960
tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1020
gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1080
ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1140
ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1200
ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1260
aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1320
tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1380
caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1440
aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1500
aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1560
agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1620
gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1680
ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1740
acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1800
ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1860
ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1920
tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1980
aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2040
aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2100
aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2160
acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2220
agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2280
tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2340
gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2400
gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2460
tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2520
agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2580
agatccagtt tggttaatta gctagcgagg gcctatttcc catgattcct tcatatttgc 2640
atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga 2700
tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa 2760
aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct 2820
tggctttata tatcttgtgg aaaggacgaa acaccgcgct gcctcagtct gcggtgtttt 2880
agagctaggc caacatgagg atcacccatg tctgcagggc ctagcaagtt aaaataaggc 2940
tagtccgtta tcaacttggc caacatgagg atcacccatg tctgcagggc caagtggcac 3000
cgagtcggtg ctttttttgg atcctaggtc ttgaaaggag tgggaattgg ctccggtgcc 3060
cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc 3120
aattgatccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac 3180
tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg 3240
aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg cgtacggcca ccatgaaaag 3300
gccggcggcc acgaaaaagg ccggccaggc aaaaaagaaa aaggacaaga agtacagcat 3360
cggcctggcc atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt 3420
gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct 3480
gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac 3540
cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag 3600
caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt 3660
ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc 3720
ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga 3780
caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca 3840
cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca 3900
gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga 3960
cgccaaggcc atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc 4020
ccagctgccc ggcgagaaga agaatggcct gttcggcaac ctgattgccc tgagcctggg 4080
cctgaccccc aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag 4140
caaggacacc tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc 4200
cgacctgttt ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag 4260
agtgaacacc gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga 4320
gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta 4380
caaagagatt ttcttcgacc agagcaagaa cggctacgcc ggctacattg acggcggagc 4440
cagccaggaa gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga 4500
ggaactgctc gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa 4560
cggcagcatc ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga 4620
agatttttac ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg 4680
catcccctac tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag 4740
aaagagcgag gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgcttc 4800
cgcccagagc ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt 4860
gctgcccaag cacagcctgc tgtacgagta cttcaccgtg tataacgagc tgaccaaagt 4920
gaaatacgtg accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc 4980
catcgtggac ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga 5040
ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt 5100
caacgcctcc ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct 5160
ggacaatgag gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga 5220
ggacagagag atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt 5280
gatgaagcag ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat 5340
caacggcatc cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg 5400
cttcgccaac agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga 5460
catccagaaa gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct 5520
ggccggcagc cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct 5580
cgtgaaagtg atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa 5640
ccagaccacc cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg 5700
catcaaagag ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca 5760
gaacgagaag ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga 5820
actggacatc aaccggctgt ccgactacga tgtggaccac atcgtgcctc agagctttct 5880
gaaggacgac tccatcgaca acaaggtgct gaccagaagc gacaaggccc ggggcaagag 5940
cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag aactactggc ggcagctgct 6000
gaacgccaag ctgattaccc agagaaagtt cgacaatctg accaaggccg agagaggcgg 6060
cctgagcgaa ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat 6120
cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaatga 6180
caagctgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg 6240
gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc 6300
ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc tggaaagcga 6360
gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca 6420
ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa 6480
gaccgagatt accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg 6540
cgaaaccggg gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct 6600
gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa 6660
agagtctatc ctgcccaaga ggaacagcga taagctgatc gccagaaaga aggactggga 6720
ccctaagaag tacggcggct tcgacagccc caccgtggcc tattctgtgc tggtggtggc 6780
caaagtggaa aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac 6840
catcatggaa agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta 6900
caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga 6960
aaacggccgg aagagaatgc tggcctctgc cggcgaactg cagaagggaa acgaactggc 7020
cctgccctcc aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg 7080
ctcccccgag gataatgagc agaaacagct gtttgtggaa cagcacaagc actacctgga 7140
cgagatcatc gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct 7200
ggacaaagtg ctgtccgcct acaacaagca ccgggataag cccatcagag agcaggccga 7260
gaatatcatc cacctgttta ccctgaccaa tctgggagcc cctgccgcct tcaagtactt 7320
tgacaccacc atcgaccgga agaggtacac cagcaccaaa gaggtgctgg acgccaccct 7380
gatccaccag agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg 7440
cgacagcgct ggaggaggtg gaagcggagg aggaggaagc ggaggaggag gtagcggacc 7500
taagaaaaag aggaaggtgg cggccgctgg atccggacgg gctgacgcat tggacgattt 7560
tgatctggat atgctgggaa gtgacgccct cgatgatttt gaccttgaca tgcttggttc 7620
ggatgccctt gatgactttg acctcgacat gctcggcagt gacgcccttg atgatttcga 7680
cctggacatg ctgattaact gtacaggcag tggagagggc agaggaagtc tgctaacatg 7740
cggtgacgtc gaggagaatc ctggcccaat ggccaagcct ttgtctcaag aagaatccac 7800
cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt 7860
cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt 7920
tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg 7980
caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg 8040
acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag 8100
tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga 8160
gggctaagaa ttcgatatca agcttatcgg taatcaacct ctggattaca aaatttgtga 8220
aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt 8280
aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa 8340
atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt 8400
gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct 8460
cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg 8520
ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc 8580
ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg 8640
gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct 8700
gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc 8760
cctttgggcc gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc 8820
aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga 8880
ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa 8940
ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca 9000
ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc 9060
tgattggcag aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg 9120
ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac 9180
ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga 9240
gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg 9300
tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 9360
cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 9420
gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 9480
tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat 9540
ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc 9600
tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg 9660
ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg 9720
gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt 9780
atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg 9840
tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc 9900
tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc 9960
gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta 10020
gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta 10080
atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg 10140
atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa 10200
aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg 10260
ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg 10320
aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 10380
aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 10440
ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc 10500
ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa 10560
gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca 10620
tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag 10680
ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg 10740
accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg 10800
gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg 10860
gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc 10920
acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg 10980
cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag 11040
gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc 11100
ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag 11160
ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc 11220
atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 11280
ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa 11340
tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 11400
cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 11460
attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 11520
tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 11580
ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 11640
gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 11700
ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 11760
cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 11820
ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 11880
accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 11940
catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 12000
gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 12060
tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 12120
agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 12180
actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 12240
gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 12300
aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 12360
gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 12420
aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 12480
atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 12540
gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 12600
atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 12660
ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 12720
cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 12780
agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 12840
cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 12900
tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 12960
agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 13020
gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 13080
gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 13140
ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 13200
tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 13260
tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 13320
gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 13380
caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 13440
atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 13500
<210> 6
<211> 13500
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 6
gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 60
atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 120
gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 180
tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 240
attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 300
atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 360
acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 420
tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 480
tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 540
attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 600
tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 660
ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 720
accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 780
gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 840
ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 900
aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 960
tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1020
gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1080
ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1140
ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1200
ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1260
aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1320
tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1380
caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1440
aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1500
aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1560
agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1620
gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1680
ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1740
acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1800
ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1860
ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1920
tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1980
aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2040
aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2100
aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2160
acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2220
agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2280
tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2340
gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2400
gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2460
tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2520
agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2580
agatccagtt tggttaatta gctagcgagg gcctatttcc catgattcct tcatatttgc 2640
atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga 2700
tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa 2760
aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct 2820
tggctttata tatcttgtgg aaaggacgaa acacccctca gtctgcggtg ggcaggtttt 2880
agagctaggc caacatgagg atcacccatg tctgcagggc ctagcaagtt aaaataaggc 2940
tagtccgtta tcaacttggc caacatgagg atcacccatg tctgcagggc caagtggcac 3000
cgagtcggtg ctttttttgg atcctaggtc ttgaaaggag tgggaattgg ctccggtgcc 3060
cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc 3120
aattgatccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac 3180
tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg 3240
aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg cgtacggcca ccatgaaaag 3300
gccggcggcc acgaaaaagg ccggccaggc aaaaaagaaa aaggacaaga agtacagcat 3360
cggcctggcc atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt 3420
gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct 3480
gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac 3540
cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag 3600
caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt 3660
ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc 3720
ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga 3780
caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca 3840
cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca 3900
gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga 3960
cgccaaggcc atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc 4020
ccagctgccc ggcgagaaga agaatggcct gttcggcaac ctgattgccc tgagcctggg 4080
cctgaccccc aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag 4140
caaggacacc tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc 4200
cgacctgttt ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag 4260
agtgaacacc gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga 4320
gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta 4380
caaagagatt ttcttcgacc agagcaagaa cggctacgcc ggctacattg acggcggagc 4440
cagccaggaa gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga 4500
ggaactgctc gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa 4560
cggcagcatc ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga 4620
agatttttac ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg 4680
catcccctac tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag 4740
aaagagcgag gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgcttc 4800
cgcccagagc ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt 4860
gctgcccaag cacagcctgc tgtacgagta cttcaccgtg tataacgagc tgaccaaagt 4920
gaaatacgtg accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc 4980
catcgtggac ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga 5040
ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt 5100
caacgcctcc ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct 5160
ggacaatgag gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga 5220
ggacagagag atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt 5280
gatgaagcag ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat 5340
caacggcatc cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg 5400
cttcgccaac agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga 5460
catccagaaa gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct 5520
ggccggcagc cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct 5580
cgtgaaagtg atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa 5640
ccagaccacc cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg 5700
catcaaagag ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca 5760
gaacgagaag ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga 5820
actggacatc aaccggctgt ccgactacga tgtggaccac atcgtgcctc agagctttct 5880
gaaggacgac tccatcgaca acaaggtgct gaccagaagc gacaaggccc ggggcaagag 5940
cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag aactactggc ggcagctgct 6000
gaacgccaag ctgattaccc agagaaagtt cgacaatctg accaaggccg agagaggcgg 6060
cctgagcgaa ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat 6120
cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaatga 6180
caagctgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg 6240
gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc 6300
ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc tggaaagcga 6360
gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca 6420
ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa 6480
gaccgagatt accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg 6540
cgaaaccggg gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct 6600
gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa 6660
agagtctatc ctgcccaaga ggaacagcga taagctgatc gccagaaaga aggactggga 6720
ccctaagaag tacggcggct tcgacagccc caccgtggcc tattctgtgc tggtggtggc 6780
caaagtggaa aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac 6840
catcatggaa agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta 6900
caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga 6960
aaacggccgg aagagaatgc tggcctctgc cggcgaactg cagaagggaa acgaactggc 7020
cctgccctcc aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg 7080
ctcccccgag gataatgagc agaaacagct gtttgtggaa cagcacaagc actacctgga 7140
cgagatcatc gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct 7200
ggacaaagtg ctgtccgcct acaacaagca ccgggataag cccatcagag agcaggccga 7260
gaatatcatc cacctgttta ccctgaccaa tctgggagcc cctgccgcct tcaagtactt 7320
tgacaccacc atcgaccgga agaggtacac cagcaccaaa gaggtgctgg acgccaccct 7380
gatccaccag agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg 7440
cgacagcgct ggaggaggtg gaagcggagg aggaggaagc ggaggaggag gtagcggacc 7500
taagaaaaag aggaaggtgg cggccgctgg atccggacgg gctgacgcat tggacgattt 7560
tgatctggat atgctgggaa gtgacgccct cgatgatttt gaccttgaca tgcttggttc 7620
ggatgccctt gatgactttg acctcgacat gctcggcagt gacgcccttg atgatttcga 7680
cctggacatg ctgattaact gtacaggcag tggagagggc agaggaagtc tgctaacatg 7740
cggtgacgtc gaggagaatc ctggcccaat ggccaagcct ttgtctcaag aagaatccac 7800
cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt 7860
cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt 7920
tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg 7980
caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg 8040
acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag 8100
tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga 8160
gggctaagaa ttcgatatca agcttatcgg taatcaacct ctggattaca aaatttgtga 8220
aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt 8280
aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa 8340
atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt 8400
gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct 8460
cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg 8520
ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc 8580
ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg 8640
gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct 8700
gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc 8760
cctttgggcc gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc 8820
aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga 8880
ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa 8940
ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca 9000
ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc 9060
tgattggcag aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg 9120
ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac 9180
ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga 9240
gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg 9300
tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 9360
cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 9420
gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 9480
tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat 9540
ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc 9600
tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg 9660
ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg 9720
gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt 9780
atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg 9840
tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc 9900
tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc 9960
gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta 10020
gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta 10080
atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg 10140
atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa 10200
aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg 10260
ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg 10320
aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 10380
aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 10440
ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc 10500
ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa 10560
gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca 10620
tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag 10680
ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg 10740
accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg 10800
gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg 10860
gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc 10920
acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg 10980
cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag 11040
gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc 11100
ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag 11160
ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc 11220
atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 11280
ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa 11340
tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 11400
cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 11460
attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 11520
tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 11580
ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 11640
gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 11700
ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 11760
cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 11820
ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 11880
accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 11940
catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 12000
gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 12060
tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 12120
agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 12180
actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 12240
gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 12300
aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 12360
gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 12420
aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 12480
atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 12540
gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 12600
atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 12660
ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 12720
cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 12780
agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 12840
cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 12900
tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 12960
agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 13020
gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 13080
gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 13140
ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 13200
tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 13260
tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 13320
gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 13380
caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 13440
atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 13500
<210> 7
<211> 13500
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 7
gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 60
atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 120
gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 180
tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 240
attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 300
atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 360
acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 420
tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 480
tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 540
attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 600
tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 660
ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 720
accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 780
gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 840
ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 900
aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 960
tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1020
gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1080
ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1140
ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1200
ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1260
aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1320
tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1380
caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1440
aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1500
aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1560
agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1620
gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1680
ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1740
acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1800
ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1860
ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1920
tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1980
aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2040
aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2100
aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2160
acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2220
agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2280
tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2340
gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2400
gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2460
tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2520
agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2580
agatccagtt tggttaatta gctagcgagg gcctatttcc catgattcct tcatatttgc 2640
atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga 2700
tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa 2760
aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct 2820
tggctttata tatcttgtgg aaaggacgaa acacccctca gtctgcggtg ggcaggtttt 2880
agagctaggc caacatgagg atcacccatg tctgcagggc ctagcaagtt aaaataaggc 2940
tagtccgtta tcaacttggc caacatgagg atcacccatg tctgcagggc caagtggcac 3000
cgagtcggtg ctttttttgg atcctaggtc ttgaaaggag tgggaattgg ctccggtgcc 3060
cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc 3120
aattgatccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac 3180
tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg 3240
aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg cgtacggcca ccatgaaaag 3300
gccggcggcc acgaaaaagg ccggccaggc aaaaaagaaa aaggacaaga agtacagcat 3360
cggcctggcc atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt 3420
gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct 3480
gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac 3540
cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag 3600
caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt 3660
ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc 3720
ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga 3780
caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca 3840
cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca 3900
gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga 3960
cgccaaggcc atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc 4020
ccagctgccc ggcgagaaga agaatggcct gttcggcaac ctgattgccc tgagcctggg 4080
cctgaccccc aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag 4140
caaggacacc tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc 4200
cgacctgttt ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag 4260
agtgaacacc gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga 4320
gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta 4380
caaagagatt ttcttcgacc agagcaagaa cggctacgcc ggctacattg acggcggagc 4440
cagccaggaa gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga 4500
ggaactgctc gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa 4560
cggcagcatc ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga 4620
agatttttac ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg 4680
catcccctac tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag 4740
aaagagcgag gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgcttc 4800
cgcccagagc ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt 4860
gctgcccaag cacagcctgc tgtacgagta cttcaccgtg tataacgagc tgaccaaagt 4920
gaaatacgtg accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc 4980
catcgtggac ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga 5040
ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt 5100
caacgcctcc ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct 5160
ggacaatgag gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga 5220
ggacagagag atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt 5280
gatgaagcag ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat 5340
caacggcatc cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg 5400
cttcgccaac agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga 5460
catccagaaa gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct 5520
ggccggcagc cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct 5580
cgtgaaagtg atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa 5640
ccagaccacc cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg 5700
catcaaagag ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca 5760
gaacgagaag ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga 5820
actggacatc aaccggctgt ccgactacga tgtggaccac atcgtgcctc agagctttct 5880
gaaggacgac tccatcgaca acaaggtgct gaccagaagc gacaaggccc ggggcaagag 5940
cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag aactactggc ggcagctgct 6000
gaacgccaag ctgattaccc agagaaagtt cgacaatctg accaaggccg agagaggcgg 6060
cctgagcgaa ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat 6120
cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaatga 6180
caagctgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg 6240
gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc 6300
ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc tggaaagcga 6360
gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca 6420
ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa 6480
gaccgagatt accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg 6540
cgaaaccggg gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct 6600
gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa 6660
agagtctatc ctgcccaaga ggaacagcga taagctgatc gccagaaaga aggactggga 6720
ccctaagaag tacggcggct tcgacagccc caccgtggcc tattctgtgc tggtggtggc 6780
caaagtggaa aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac 6840
catcatggaa agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta 6900
caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga 6960
aaacggccgg aagagaatgc tggcctctgc cggcgaactg cagaagggaa acgaactggc 7020
cctgccctcc aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg 7080
ctcccccgag gataatgagc agaaacagct gtttgtggaa cagcacaagc actacctgga 7140
cgagatcatc gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct 7200
ggacaaagtg ctgtccgcct acaacaagca ccgggataag cccatcagag agcaggccga 7260
gaatatcatc cacctgttta ccctgaccaa tctgggagcc cctgccgcct tcaagtactt 7320
tgacaccacc atcgaccgga agaggtacac cagcaccaaa gaggtgctgg acgccaccct 7380
gatccaccag agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg 7440
cgacagcgct ggaggaggtg gaagcggagg aggaggaagc ggaggaggag gtagcggacc 7500
taagaaaaag aggaaggtgg cggccgctgg atccggacgg gctgacgcat tggacgattt 7560
tgatctggat atgctgggaa gtgacgccct cgatgatttt gaccttgaca tgcttggttc 7620
ggatgccctt gatgactttg acctcgacat gctcggcagt gacgcccttg atgatttcga 7680
cctggacatg ctgattaact gtacaggcag tggagagggc agaggaagtc tgctaacatg 7740
cggtgacgtc gaggagaatc ctggcccaat ggccaagcct ttgtctcaag aagaatccac 7800
cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt 7860
cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt 7920
tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg 7980
caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg 8040
acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag 8100
tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga 8160
gggctaagaa ttcgatatca agcttatcgg taatcaacct ctggattaca aaatttgtga 8220
aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt 8280
aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa 8340
atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt 8400
gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct 8460
cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg 8520
ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc 8580
ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg 8640
gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct 8700
gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc 8760
cctttgggcc gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc 8820
aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga 8880
ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa 8940
ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca 9000
ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc 9060
tgattggcag aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg 9120
ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac 9180
ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga 9240
gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg 9300
tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 9360
cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 9420
gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 9480
tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat 9540
ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc 9600
tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg 9660
ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg 9720
gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt 9780
atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg 9840
tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc 9900
tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc 9960
gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta 10020
gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta 10080
atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg 10140
atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa 10200
aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg 10260
ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg 10320
aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 10380
aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 10440
ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc 10500
ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa 10560
gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca 10620
tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag 10680
ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg 10740
accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg 10800
gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg 10860
gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc 10920
acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg 10980
cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag 11040
gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc 11100
ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag 11160
ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc 11220
atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 11280
ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa 11340
tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 11400
cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 11460
attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 11520
tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 11580
ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 11640
gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 11700
ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 11760
cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 11820
ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 11880
accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 11940
catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 12000
gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 12060
tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 12120
agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 12180
actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 12240
gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 12300
aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 12360
gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 12420
aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 12480
atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 12540
gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 12600
atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 12660
ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 12720
cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 12780
agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 12840
cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 12900
tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 12960
agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 13020
gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 13080
gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 13140
ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 13200
tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 13260
tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 13320
gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 13380
caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 13440
atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 13500
<210> 8
<211> 8865
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 8
tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 60
cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 120
tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 180
ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 240
agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 300
agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 360
ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 420
cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 480
gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 540
tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 600
agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 660
cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 720
caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 780
aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 840
aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 900
caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 960
gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1020
cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1080
ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1140
aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1200
tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1260
aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1320
gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1380
cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1440
gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1500
ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1560
actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1620
gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1680
aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1740
ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1800
tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1860
actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1920
cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1980
cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2040
gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2100
aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2160
acagcagaga tccagtttat cgatacgcgt gggagttccg cgttacataa cttacggtaa 2220
atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2280
ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2340
aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2400
tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc 2460
ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2520
agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2580
ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2640
acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2700
gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2760
tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2820
ctcaagcttc gaattcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt 2880
gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 2940
gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 3000
gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag 3060
ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta 3120
cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 3180
gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 3240
ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 3300
catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga 3360
ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 3420
cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa 3480
cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 3540
catggacgag ctgtacaagg gatccgacta caaggatgac gatgacaagg attacaaaga 3600
cgacgatgat aaggactata aggatgatga cgacaaatct agataattct accgggtagg 3660
ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg 3720
gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc 3780
ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt 3840
cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac 3900
tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag 3960
cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc 4020
cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg 4080
cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc 4140
tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc 4200
ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac 4260
taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg 4320
caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac 4380
ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc 4440
gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg 4500
gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc 4560
gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag 4620
gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc 4680
ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc 4740
acctggtgca tgacccgcaa gcccggtgcc tgaccgcgtc tggaacaatc aacctctgga 4800
ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 4860
tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 4920
ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 4980
gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 5040
caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 5100
actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 5160
ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 5220
ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 5280
tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 5340
gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac 5400
ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc 5460
tggctagaag cacaagagga ggaggaggtg ggtttttcca gtcacacctc aggtaccttt 5520
aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagagggg 5580
actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca 5640
cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc 5700
actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc 5760
caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc 5820
ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg 5880
agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc 5940
cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag 6000
atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct 6060
gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc 6120
cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc 6180
tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta 6240
ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct tgacattgct 6300
agcgttttac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct 6360
gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 6420
aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 6480
gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 6540
agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 6600
gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 6660
gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 6720
cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 6780
aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 6840
tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 6900
ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 6960
ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 7020
cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 7080
ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 7140
gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt 7200
atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 7260
aaacaaacca ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa 7320
aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 7380
aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 7440
ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 7500
agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 7560
atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 7620
cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 7680
aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 7740
cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 7800
aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 7860
ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 7920
gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 7980
ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 8040
tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 8100
tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 8160
ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 8220
tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 8280
agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 8340
acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 8400
ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 8460
gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt 8520
gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 8580
agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 8640
tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata 8700
ccctattacc actgccaatt acctagtggt ttcatttact ctaaacctgt gattcctctg 8760
aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttt 8820
ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt 8865
<210> 9
<211> 8617
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 9
tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 60
cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 120
tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 180
ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 240
agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 300
agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 360
ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 420
cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 480
gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 540
tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 600
agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 660
cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 720
caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 780
aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 840
aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 900
caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 960
gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1020
cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1080
ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1140
aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1200
tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1260
aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1320
gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1380
cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1440
gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1500
ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1560
actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1620
gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1680
aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1740
ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1800
tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1860
actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1920
cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1980
cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2040
gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2100
aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2160
acagcagaga tccagtttat cgatacgcgt ctgcagaggg ccctgcgtat gagtgcaagt 2220
gggttttagg accaggatga ggcggggtgg gggtgcctac ctgacgaccg accccgaccc 2280
actggacaag cacccaaccc ccattcccca aattgcgcat cccctatcag agagggggag 2340
gggaaacagg atgcggcgag gcgcgtgcgc actgccagct tcagcaccgc ggacagtgcc 2400
ttcgcccccg cctggcggcg cgcgccaccg ccgcctcagc actgaaggcg cgctgacgtc 2460
actcgccggt cccccgcaaa ctccccttcc cggccacctt ggtcgcgtcc gcgccgccgc 2520
cggcccagcc ggaccgcacc acgcgaggcg cgagataggg gggcacgggc gcgaccatct 2580
gcgctgcggc gccggcgact cagcgctgcc tcagtctgcg gtgggcagcg gaggagtcgt 2640
gtcgtgcctg agagcgcagt cgagaagaat tcgccaccat ggtgagcaag ggcgaggagg 2700
ataacatggc ctctctccca gcgacacatg agttacacat ctttggctcc atcaacggtg 2760
tggactttga catggtgggt cagggcaccg gcaatccaaa tgatggttat gaggagttaa 2820
acctgaagtc caccaagggt gacctccagt tctccccctg gattctggtc cctcatatcg 2880
ggtatggctt ccatcagtac ctgccctacc ctgacgggat gtcgcctttc caggccgcca 2940
tggtagatgg ctccggatac caagtccatc gcacaatgca gtttgaagat ggtgcctccc 3000
ttactgttaa ctaccgctac acctacgagg gaagccacat caaaggagag gcccaggtga 3060
aggggactgg tttccctgct gacggtcctg tgatgaccaa ctcgctgacc gctgcggact 3120
ggtgcaggtc gaagaagact taccccaacg acaaaaccat catcagtacc tttaagtgga 3180
gttacaccac tggaaatggc aagcgctacc ggagcactgc gcggaccacc tacacctttg 3240
ccaagccaat ggcggctaac tatctgaaga accagccgat gtacgtgttc cgtaagacgg 3300
agctcaagca ctccaagacc gagctcaact tcaaggagtg gcaaaaggcc tttaccgatg 3360
tgatgggcat ggacgagctg tacaagtaat ctagataatt ctaccgggta ggggaggcgc 3420
ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca 3480
caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt 3540
tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg 3600
ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg 3660
tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat 3720
agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg 3780
ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat 3840
tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc 3900
tttcgacctg cagcccaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac 3960
ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc 4020
cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact 4080
cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc 4140
ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg 4200
cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct 4260
cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc 4320
cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga 4380
gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga 4440
gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg 4500
catgacccgc aagcccggtg cctgaccgcg tctggaacaa tcaacctctg gattacaaaa 4560
tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg 4620
ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct 4680
tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg 4740
gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct 4800
gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg 4860
ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg 4920
tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc 4980
tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc 5040
gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc 5100
ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa 5160
acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga 5220
agcacaagag gaggaggagg tgggtttttc cagtcacacc tcaggtacct ttaagaccaa 5280
tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag 5340
ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag 5400
gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct 5460
ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag 5520
gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag 5580
aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc 5640
atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg 5700
actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca 5760
tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg 5820
gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 5880
cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 5940
ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat 6000
ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg ctagcgtttt 6060
accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 6120
ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 6180
gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 6240
gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 6300
tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 6360
gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 6420
ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 6480
ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 6540
acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 6600
tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 6660
ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 6720
ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 6780
ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 6840
actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 6900
gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 6960
tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 7020
caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 7080
tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 7140
ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 7200
aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 7260
atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 7320
ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 7380
tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 7440
agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 7500
taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 7560
tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 7620
cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 7680
ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 7740
tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 7800
tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 7860
cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 7920
tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 7980
gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 8040
tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 8100
atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 8160
tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 8220
cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg 8280
cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 8340
tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga 8400
tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta 8460
ccactgccaa ttacctagtg gtttcattta ctctaaacct gtgattcctc tgaattattt 8520
tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt ttttaaagaa 8580
attgtatttg ttaaatatgt actacaaact tagtagt 8617
<210> 10
<211> 6551
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 10
acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 60
acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 120
cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 180
attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 240
tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 300
agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 360
ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 420
cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 480
tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 540
gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 600
aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 660
aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 720
agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 780
atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 840
gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 900
taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 960
acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1020
cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1080
ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1140
tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1200
gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1260
aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1320
gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1380
aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1440
ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1500
acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1560
ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1620
agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1680
tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1740
tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1800
taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1860
aacagacata caaactaaag aattacaaaa acaaattaac aaaattcaaa attttatcga 1920
tactagtacg cgtggagcta gttattaata gtaatcaatt acggggtcat tagttcatag 1980
cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc 2040
caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg 2100
gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca 2160
tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc 2220
ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt 2280
attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata 2340
gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt 2400
ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca 2460
aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg 2520
tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg 2580
atccagcctc cgtcgacacc ggtggatccg aattcgctag cctcgagggt tatcgataat 2640
caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 2700
tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 2760
gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 2820
cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 2880
tggggcattg ccaccacctg tcagctcctt tccggaactt tcgctttccc cctccctatt 2940
gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 3000
ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc 3060
tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 3120
ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc 3180
cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgggta 3240
cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag 3300
gggggactgg aagggctaat tcactcccaa cgaagataag atctgctttt tgcttgtact 3360
gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 3420
ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 3480
tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 3540
agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca 3600
gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 3660
acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 3720
atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg 3780
cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 3840
cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca 3900
gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 3960
tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 4020
gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 4080
tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 4140
ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 4200
cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 4260
gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 4320
tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 4380
agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 4440
tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 4500
cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 4560
ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 4620
ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 4680
ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 4740
ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 4800
cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 4860
gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 4920
atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 4980
ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 5040
gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 5100
tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 5160
ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 5220
taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 5280
gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 5340
gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 5400
ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 5460
aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 5520
gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 5580
cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 5640
actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 5700
caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 5760
gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 5820
ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 5880
caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 5940
tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 6000
gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 6060
cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa 6120
ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct 6180
gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac 6240
aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg 6300
catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg 6360
taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag 6420
ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 6480
ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 6540
gtgccaagct g 6551
<210> 11
<211> 9062
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 11
acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 60
acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 120
cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 180
attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 240
tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 300
agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 360
ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 420
cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 480
tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 540
gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 600
aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 660
aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 720
agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 780
atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 840
gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 900
taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 960
acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1020
cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1080
ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1140
tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1200
gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1260
aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1320
gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1380
aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1440
ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1500
acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1560
ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1620
agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1680
tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1740
tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1800
taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1860
aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1920
tactagtacg cgtggagcta gttattaata gtaatcaatt acggggtcat tagttcatag 1980
cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc 2040
caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg 2100
gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca 2160
tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc 2220
ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt 2280
attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata 2340
gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt 2400
ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca 2460
aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg 2520
tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg 2580
atccagcctc cgtcgacgcc accatggctt caaactttac tcagttcgtg ctcgtggaca 2640
atggtgggac aggggatgtg acagtggctc cttctaattt cgctaatggg gtggcagagt 2700
ggatcagctc caactcacgg agccaggcct acaaggtgac atgcagcgtc aggcagtcta 2760
gtgcccagaa gagaaagtat accatcaagg tggaggtccc caaagtggct acccagacag 2820
tgggcggagt cgaactgcct gtcgccgctt ggaggtccta cctgaacatg gagctcacta 2880
tcccaatttt cgctaccaat tctgactgtg aactcatcgt gaaggcaatg caggggctcc 2940
tcaaagacgg taatcctatc ccttccgcca tcgccgctaa ctcaggtatc tacagcgctg 3000
gaggaggtgg aagcggagga ggaggaagcg gaggaggagg tagcggacct aagaaaaaga 3060
ggaaggtggc ggccgctgga tccccttcag ggcagatcag caaccaggcc ctggctctgg 3120
cccctagctc cgctccagtg ctggcccaga ctatggtgcc ctctagtgct atggtgcctc 3180
tggcccagcc acctgctcca gcccctgtgc tgaccccagg accaccccag tcactgagcg 3240
ctccagtgcc caagtctaca caggccggcg aggggactct gagtgaagct ctgctgcacc 3300
tgcagttcga cgctgatgag gacctgggag ctctgctggg gaacagcacc gatcccggag 3360
tgttcacaga tctggcctcc gtggacaact ctgagtttca gcagctgctg aatcagggcg 3420
tgtccatgtc tcatagtaca gccgaaccaa tgctgatgga gtaccccgaa gccattaccc 3480
ggctggtgac cggcagccag cggccccccg accccgctcc aactcccctg ggaaccagcg 3540
gcctgcctaa tgggctgtcc ggagatgaag acttctcaag catcgctgat atggacttta 3600
gtgccctgct gtcacagatt tcctctagtg ggcagggagg aggtggaagc ggcttcagcg 3660
tggacaccag tgccctgctg gacctgttca gcccctcggt gaccgtgccc gacatgagcc 3720
tgcctgacct tgacagcagc ctggccagta tccaagagct cctgtctccc caggagcccc 3780
ccaggcctcc cgaggcagag aacagcagcc cggattcagg gaagcagctg gtgcactaca 3840
cagcgcagcc gctgttcctg ctggaccccg gctccgtgga caccgggagc aacgacctgc 3900
cggtgctgtt tgagctggga gagggctcct acttctccga aggggacggc ttcgccgagg 3960
accccaccat ctccctgctg acaggctcgg agcctcccaa agccaaggac cccactgtct 4020
cctgtacagg cagtggagag ggcagaggaa gtctgctaac atgcggtgac gtcgaggaga 4080
atcctggccc aaccatgaaa aagcctgaac tcaccgctac ctctgtcgag aagtttctga 4140
tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc cgagggcgaa gaatctcggg 4200
ctttcagctt cgatgtggga gggcgtggat atgtcctgcg ggtgaatagc tgcgccgatg 4260
gtttctacaa agatcgctat gtttatcggc actttgcatc cgccgctctc cctattcccg 4320
aagtgcttga cattggggag ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac 4380
agggtgtcac cttgcaagac ctgcctgaaa ccgaactgcc cgctgttctc cagcccgtcg 4440
ccgaggccat ggatgccatc gctgccgccg atcttagcca gaccagcggg ttcggcccat 4500
tcggacctca aggaatcggt caatacacta catggcgcga tttcatctgc gctattgctg 4560
atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcc tccgtcgccc 4620
aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc 4680
acgccgattt cggctccaac aatgtcctga ccgacaatgg ccgcataaca gccgtcattg 4740
actggagcga ggccatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga 4800
ggccctggtt ggcttgtatg gagcagcaga cccgctactt cgagcggagg catcccgagc 4860
ttgcaggatc tcctcggctc cgggcttata tgctccgcat tggtcttgac caactctatc 4920
agagcttggt tgacggcaat ttcgatgatg cagcttgggc tcagggtcgc tgcgacgcaa 4980
tcgtccggtc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgctgccg 5040
tctggaccga tggctgtgtg gaagtgctcg ccgatagtgg aaacagacgc cccagcactc 5100
gtcctagggc aaaggatctg cagtaagcta gcctcgaggg ttatcgataa tcaacctctg 5160
gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 5220
tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 5280
ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 5340
aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 5400
gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 5460
gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 5520
aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc 5580
acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 5640
cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct 5700
cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgggt acctttaaga 5760
ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg 5820
gaagggctaa ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc 5880
tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 5940
cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 6000
ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt 6060
tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag 6120
aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 6180
acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 6240
tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc 6300
cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 6360
agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc 6420
caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 6480
cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 6540
aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 6600
agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 6660
ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 6720
ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 6780
tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 6840
tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 6900
gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 6960
ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 7020
tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 7080
agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 7140
atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 7200
acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 7260
actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 7320
tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 7380
tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 7440
tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 7500
tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 7560
caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 7620
cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 7680
agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 7740
acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 7800
gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 7860
ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 7920
tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 7980
ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 8040
tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 8100
attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 8160
agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 8220
ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 8280
ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 8340
cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 8400
gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 8460
tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 8520
tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 8580
tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 8640
tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc 8700
agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc 8760
agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc 8820
agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 8880
aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg 8940
tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa 9000
gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc 9060
tg 9062

Claims (10)

1.一种在工具细胞中激活hSyn启动子的方法，其特征在于，采用CRISPR-Cas9 SAM系统。
2.根据权利要求1所述的方法，其特征在于，包括：设计合成sgRNA，所述sgRNA为sgRNA1、sgRNA2或sgRNA3，其核苷酸序列分别如SEQ ID NO：1、SEQ ID NO：2和SEQ ID NO：3所示。
3.根据权利要求2所述的方法，其特征在于，包括：
S1：合成权利要求2所述的sgRNA；
S2：将含荧光蛋白标记的慢病毒质粒导入工具细胞中得到工具细胞过表达稳定株；
S3：将dCas9--VP64融合表达质粒、sgRNA质粒和激活辅助蛋白p65-HSF1表达质粒共同导入工具细胞过表达稳定株中，观察荧光。
4.根据权利要求3所述的方法，其特征在于，所述sgRNA质粒的构建方法为：将sgRNA导入空载载体pLenti-U6-gRNA-MS2-EF1a-dCas9-VP64-T2A-BSD中得到sgRNA质粒。
5.根据权利要求3所述的方法，其特征在于，所述S2包括步骤S21：构建含荧光蛋白标记的慢病毒质粒。
6.根据权利要求5所述的方法，其特征在于，所述S21包括以下步骤：
S211：将hSyn-mNeonGreen核苷酸序列连入空载载体pLenti-CMV-EGFP-3FLAG-PGK-Puro中即得到含荧光蛋白标记的慢病毒质粒。
7.根据权利要求1-6任意一项所述的方法在基因工程领域中的应用。
8.CRISPR-Cas9 SAM系统在工具细胞中激活hSyn启动子的应用。
9.一种用于激活hSyn启动子的sgRNA，其特征在于，所述sgRNA为sgRNA1、sgRNA2或sgRNA3，其核苷酸序列分别如SEQ ID NO：1、SEQ ID NO：2和SEQ ID NO：3所示。
10.一种试剂盒，其特征在于，所述试剂盒包括权利要求9所述的sgRNA。
CN201910629189.3A 2019-07-12 2019-07-12 一种在工具细胞中激活hSyn启动子的方法及其应用 Active CN110305902B (zh)

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