CN110305867A - 调控脂肪干细胞干性维持能力的方法及其试剂 - Google Patents
调控脂肪干细胞干性维持能力的方法及其试剂 Download PDFInfo
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Abstract
Description
编号 | 序列 | 起始 |
siRNA1 | tgctcttgggtaaggaatgc | 421 |
siRNA2 | ggcagatcaacaagacagaa | 902 |
siRNA3 | tagagaagcaagagcaaaca | 1408 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111808861A (zh) * | 2020-07-22 | 2020-10-23 | 青岛思拓新源细胞医学有限公司 | 一种lncRNA在氧化应激引起的脐血间充质干细胞凋亡中的应用 |
Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9441224B1 (en) * | 2013-06-27 | 2016-09-13 | California Institute Of Technology | Long noncoding RNAS and cell reprogramming and differentiation |
CN107320726A (zh) * | 2017-07-11 | 2017-11-07 | 山东大学 | LncRNA‑TUG1在制备调控牙周膜干细胞的干性维持能力的药物中的应用 |
CN107354127A (zh) * | 2017-07-11 | 2017-11-17 | 山东大学 | LncRNA‑TUG1在调控PDLSCs成骨分化及组织再生中的作用 |
CN107475402A (zh) * | 2017-09-11 | 2017-12-15 | 青海七彩花生物科技有限公司 | 一种用于早期预示骨髓间充质干细胞成骨分化潜能的基因检测试剂盒 |
US9862943B1 (en) * | 2013-06-27 | 2018-01-09 | California Institute Of Technology | Long noncoding RNAs and cell reprogramming and differentiation |
CN108441548A (zh) * | 2018-04-01 | 2018-08-24 | 南京千年健干细胞基因工程有限公司 | 一种基于长链非编码rna的鉴定脐带间充质干细胞传代次数的基因检测试剂盒 |
CN109224130A (zh) * | 2018-10-09 | 2019-01-18 | 中国人民解放军陆军军医大学 | 长链非编码RNA lnc-HCAR在制备骨修复体系中的应用及骨修复体系和制备方法 |
CN109402248A (zh) * | 2018-11-08 | 2019-03-01 | 中国医学科学院北京协和医院 | 一种青少年特发性脊柱侧凸的lncRNA标志物lncAIS及其应用 |
CN109568343A (zh) * | 2018-11-08 | 2019-04-05 | 中国医学科学院北京协和医院 | 促进骨髓间充质干细胞成骨分化的生物制剂 |
KR20190063333A (ko) * | 2017-11-29 | 2019-06-07 | 연세대학교 산학협력단 | 유도만능 줄기세포 분화 특이적 lncRNA, 및 이의 용도 |
-
2019
- 2019-07-10 CN CN201910622120.8A patent/CN110305867B/zh active Active
Patent Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9441224B1 (en) * | 2013-06-27 | 2016-09-13 | California Institute Of Technology | Long noncoding RNAS and cell reprogramming and differentiation |
US9862943B1 (en) * | 2013-06-27 | 2018-01-09 | California Institute Of Technology | Long noncoding RNAs and cell reprogramming and differentiation |
CN107320726A (zh) * | 2017-07-11 | 2017-11-07 | 山东大学 | LncRNA‑TUG1在制备调控牙周膜干细胞的干性维持能力的药物中的应用 |
CN107354127A (zh) * | 2017-07-11 | 2017-11-17 | 山东大学 | LncRNA‑TUG1在调控PDLSCs成骨分化及组织再生中的作用 |
CN107475402A (zh) * | 2017-09-11 | 2017-12-15 | 青海七彩花生物科技有限公司 | 一种用于早期预示骨髓间充质干细胞成骨分化潜能的基因检测试剂盒 |
KR20190063333A (ko) * | 2017-11-29 | 2019-06-07 | 연세대학교 산학협력단 | 유도만능 줄기세포 분화 특이적 lncRNA, 및 이의 용도 |
CN108441548A (zh) * | 2018-04-01 | 2018-08-24 | 南京千年健干细胞基因工程有限公司 | 一种基于长链非编码rna的鉴定脐带间充质干细胞传代次数的基因检测试剂盒 |
CN109224130A (zh) * | 2018-10-09 | 2019-01-18 | 中国人民解放军陆军军医大学 | 长链非编码RNA lnc-HCAR在制备骨修复体系中的应用及骨修复体系和制备方法 |
CN109402248A (zh) * | 2018-11-08 | 2019-03-01 | 中国医学科学院北京协和医院 | 一种青少年特发性脊柱侧凸的lncRNA标志物lncAIS及其应用 |
CN109568343A (zh) * | 2018-11-08 | 2019-04-05 | 中国医学科学院北京协和医院 | 促进骨髓间充质干细胞成骨分化的生物制剂 |
Non-Patent Citations (3)
Title |
---|
MAJEWSKA等: "Homo sapiens clone TCONS_00183316 lncRNA, complete sequence", 《GENEBANK》 * |
MARTA MAJEWSKA等: "Preliminary RNA-Seq Analysis of Long Non-Coding RNAs Expressed in Human Term Placenta", 《INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES》 * |
罗金莲等: "间充质干细胞干性维持及多向分化相关LncRNA的研究进展", 《重庆理工大学学报(自然科学)》 * |
Cited By (2)
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CN111808861A (zh) * | 2020-07-22 | 2020-10-23 | 青岛思拓新源细胞医学有限公司 | 一种lncRNA在氧化应激引起的脐血间充质干细胞凋亡中的应用 |
CN111808861B (zh) * | 2020-07-22 | 2021-04-13 | 青岛思拓新源细胞医学有限公司 | 一种lncRNA在氧化应激引起的脐血间充质干细胞凋亡中的应用 |
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