CN110302111A

CN110302111A - A kind of anti-oxidant gel mask, preparation method and application

Info

Publication number: CN110302111A
Application number: CN201910672186.8A
Authority: CN
Inventors: 汪祖华; 吕享; 徐峰; 周英; 谭申宇; 尚林林
Original assignee: Guizhou University of Traditional Chinese Medicine
Current assignee: Guizhou University of Traditional Chinese Medicine
Priority date: 2019-07-24
Filing date: 2019-07-24
Publication date: 2019-10-08
Anticipated expiration: 2039-07-24
Also published as: CN110302111B

Abstract

The present invention relates to a kind of anti-oxidant gel masks, preparation method and application, the facial mask consists of the following compositions: 0.5~4.0g of extractive of perilla, 0.5~4.0g of Rhizoma Polygonati extract, III 1.0~2.0g of methylcellulose, 1.0~3.0g of sodium alginate, 4.0~10g of glycerol, 1.0~3.0g of rilanit special, 1~3ml of oiliness ammonia ketone, 1~3ml of Tween-80, ethylparaben 0.2g, distilled water 150ml, by weighing methylcellulose III, sodium alginate, distilled water is added, is dissolved by heating in magnetic stirring apparatus；It takes glycerol and rilanit special to be placed in a beaker, stirs evenly, stand overnight defoaming, obtain bare substrate；Extractive of perilla and Rhizoma Polygonati extract is added, stirs evenly, oiliness azone and tween is added, stirs evenly, with distilled water constant volume, gel mask of the invention can be obtained, it is good to no skin irritation, moistening effect that gel mask of the invention has many advantages, such as.

Description

A kind of anti-oxidant gel mask, preparation method and application

Technical field

The present invention relates to Chinese medicinal skin care product technical fields, and in particular to a kind of anti-oxidant gel mask, preparation method and answers With.

Background technique

" Li Yue Pianwen, Rhymed Discourse for Topical Remedies " medium cloud: " the i.e. interior reason controlled of the reason controlled outside, the medicine controlled outside that is, the interior medicine controlled, the different person's method of institute Ear ... and control in capable of mending not as good as this ".Face mask of traditional Chinese medicine is exactly the concrete form of Chinese medicine external, in some common maxillofacial diseases As whelk, dark sore treatment in terms of, have certain curative effect.For traditional facial mask, face mask of traditional Chinese medicine not spirituosity, swash The chemical components such as element, artificial coloring, preservative, with tcm characteristic method and reasonable prescription ratio, using in Chinese medicine effectively at Point, it is suitable for very much making cosmetics addition to reach improvement circulation, cleaning face, beautification skin, eliminate the cosmetic results such as facial defect Agent, it is, more natural, milder safer as the cosmetics of raw material compared to using synthetic, effective more longlasting.Modern Chinese herbal medicine face The market exploitation of film has prospect very much.

Secondly, pertinent literature shows that the antioxidant activity of Chinese medical extract generally compared with BHA and BHT high, therefore will have anti- The Chinese medical extract of oxidation is applied in face mask of traditional Chinese medicine, will be the important of further herbal mixture facial mask anti-oxidation efficacy Approach.The main effect or moisturizing of general facial mask, by the screening and formulation optimization of Chinese medicine, can in face mask of traditional Chinese medicine moisturizing, Make face mask of traditional Chinese medicine that there is preferable anti-oxidation function on the basis of antiallergy, the acne that disappears, whitening.It is anti-oxidant to refer to resisting oxidation free radical Abbreviation, English Antioxidant, mainly a kind of performance of flight against senium of human body, human skin with extraneous because persistently connect Touching includes that factors, the free radicals such as breathing (oxidation reaction), outside contamination, radioactive ray irradiation constantly generate in human body.Science is ground Study carefully and show cancer, aging or Other diseases are mostly relevant with the generation of excessive free radicals.Studying anti-oxidant can effectively press down System overcomes its bring to endanger, so anti-oxidant be classified as one of most important R&D direction by health care product, Management of Cosmetics Enterprises, and One of most important function demand in market.

By consulting literatures it is found that purple perilla (Perilla frutescens) is Labiatae annual herb plant, have Special fragrance, is the traditional Chinese medicine in China.It is used as medicine respectively with leaf, stalk, fruit, wherein perilla leaf has relieving exterior syndrome and dispelling cold, promoting the circulation of qi The effect of stomach function regulating is one of 60 kinds of " integration of drinking and medicinal herbs " kinds that the Ministry of Public Health, China promulgates in the first batch, has very high utility value.I State is resourceful, and there is cultivation in many areas, and Sichuan province purple perilla yield is high, and numerous in variety.It is mainly used in the north with oil Based on；South is also served as fragrance and is eaten based on medicinal.And modern pharmacology show purple perilla have anti-oxidant, anti-inflammatory antiallergy, Fat-reducing liver-protecting, anticancer, enhancing memory and other effects.Antioxidant can remove active oxygen excessive in vivo, maintain active oxygen generation The balance thanked prevents the generation of disease, moreover it is possible to prevent food spoilage, play a very important role to people's lives tool.Purple perilla is anti- Oxidation activity is more significant, and Rosmarinic acid is one of active constituent of purple perilla, has been reported that the Rosmarinic acid that it contains is removed freely The ability of base ion is better than ascorbic acid, and extract antioxidant activity is also significantly stronger than vitamin C.So purple perilla is as one The plant in the natural antioxidant source of kind, has higher utility value.Rhizoma polygonati is the traditional Chinese herbal medicine in China, early in ancient books It is just on the books in " bright doctor not record ".Rhizoma polygonati is a kind of perennial herbaceous plant, is classified as Liliaceae Polygonatum (Polygonatum).Rhizoma polygonati is distributed mainly on the ground such as Shaanxi, Gansu, Hebei, Yunnan and Guizhou in the distributed more widely of China.It is yellow Contain multiple components in the water extract of essence, wherein polysaccharide is one of important biologically active substance.Rhizoma polygonati is more Sugar have adjust immune function, antibacterial, anti-aging, it is antifatigue, antitumor, anti-oxidant the effects of.The coptis is conventional Chinese medicine, is mainly contained Have isoquinoline alkaloid, lignanoid, cumarin, flavones, terpene, steroidal, organic acid, volatile oil, polysaccharide etc. it is a variety of chemistry at Point, there are the pharmacological activity such as hypoglycemic, antibacterial, anti-oxidant, anti-inflammatory, antitumor, Adjust-blood lipid, anti-arrhythmia.

In recent years, with the high speed development of economic society, the raising of living standard and quality, people increasingly focus on skin Maintenance, face mask of traditional Chinese medicine because its composition is natural, curative effect is reliable, do not stimulate mildly, application it is simple, it is cheap and by vast User like and highly recognition.Especially for group of college students and office worker, live a fast and stressful life, poor sleep, Along with electronic product is used for a long time, skin water shortage is serious, and skin is easy to aging.And medicine cosmetic its certain medical value is won The height for obtaining them is trusted, and the medicine cosmetic with anti-oxidant, anti-aging, the acne that disappears reparation and other effects is more favored.

In China, Chinese medicine cosmetic is with a long history, in the ancient times such as the Classic of Mountains and Rivers, Shennong's Herbal, Compendium of Material Medica It cures in masterpiece about the record of Chinese medicine cosmetic.Face mask of traditional Chinese medicine is one of Chinese medicine cosmetic dosage form and Chinese medicine One of most effective Chinese medicine beauty skin care product, exclusive skin care mechanism in cosmetic make it be increasingly becoming Chinese medicine makeup Very active field in product research.Currently, Chinese medicine cosmetic market is just becoming a popular industry gradually.

Gelling agent is a kind of novel pharmaceutical formulation of rising in recent years, and external research is relatively early, development is very fast.Domestic development from Hospital preparation starting, obtains national certification there are many gelling agent, shows the more advantage effectiveness of gelling agent.Gelling agent can be grown Time mutually depends on affected part, increase effect substance Transdermal absorption, using rear easy cleaning and stability it is good, be topical agent dosage form In one preferably select, therefore gel washing facial mask on face mask of traditional Chinese medicine application have one for the facial mask of other forms Fixed advantage.

Chinese Patent Application No. CN201810380368.3, denomination of invention: a kind of facial mask organic additive formula, the present invention Disclose a kind of facial mask organic additive formula, including aloe, Poria cocos, Radix Paeoniae Alba, lotus root starch, Huang Shi, the bletilla striata, rhizoma atractylodis, cottonrose hibiscus leaf, purple Soviet Union, borneol, daybreak powder, thizoma curculiginis, the fruit of glossy privet, cordyceps sinensis and rhizoma polygonati.The present invention is organic nuisanceless, can effectively improve moistening for facial mask Effect promotes the maintainability of facial mask.

It is to invent organic nuisanceless, energy according to the content that Chinese Patent Application No. CN201810380368.3 specification is recorded The effective moisturizing effect for improving facial mask, promotes the maintainability of facial mask.But it has the following deficiencies: 1. without anti-oxidation function；② Only facial mask additive；It 3. being not gel mask, therefore cannot mutually be depended on affected part for a long time, not can increase effect accordingly The Transdermal absorption amount of substance, drug effect is impacted, and stability is also poor；4. ingredient is more, higher cost；5. specific system is not known Preparation Method, curative effect are indefinite.

In order to solve the above-mentioned technical problem team of the present invention, is guidance with traditional Chinese medical theory, based on basic theory of tcm, On the basis of following the innovation etiology of differentiation of tcm, and modern medicine method and means are used for reference, has been extracted with purple perilla Object, Rhizoma Polygonati extract, coptis extract be main medicinal material and excipient matrix appropriate carry out experiments of single factor, orthogonal experiment method with And quality evaluation is carried out to facial mask according to " Chinese Pharmacopoeia " (2015) version, preferably the optimal prescription of facial mask matches out, to prepare Compound perilla gel mask out.The gel wash facial mask have potential moisturizing, pro-skin, whitening, antiallergy, it is anti-oxidant, anti-inflammatory, Bacteriostasis.Application of this experiment to the research achievement of compound perilla facial mask to propulsion natural resources of Chinese medicinal materials and face mask of traditional Chinese medicine in medicine adornment There is substantive application value, also help the exploitation of the tcm products such as purple perilla, rhizoma polygonati, greatly promotes its surcharge.

Summary of the invention

The purpose of the present invention is overcome following defect in the prior art: 1. without anti-oxidation function；2. being not gel face Film, therefore cannot mutually be depended on affected part for a long time, not can increase the Transdermal absorption amount of effect substance accordingly, drug effect is impacted, Stability is also poor；3. ingredient is more, higher cost；4. specific preparation method is not known, curative effect is indefinite.

It is a further object of the present invention to provide one kind to have moisturizing, oxidation resistant gel mask.

The preparation method with moisturizing, oxidation resistant gel mask that it is a further object of the present invention to provide a kind of.

It is another object of the present invention to the gel masks of research and development in moisturizing, the application of anti-oxidant aspect.

A kind of anti-oxidant gel mask of the present invention is made of following raw material: 0.5~4.0g of extractive of perilla, rhizoma polygonati 0.5~4.0g of extract.

Facial mask of the present invention further includes that pharmaceutically acceptable dosage form is made with pharmaceutically acceptable auxiliary material.

Auxiliary material of the present invention are as follows: III 1.0~2.0g of methylcellulose, 1.0~3.0g of sodium alginate, glycerol 4.0~ 10g, 1.0~3.0g of rilanit special, 1~3ml of oiliness ammonia ketone, 1~3ml of Tween-80, ethylparaben 0.2g, distilled water 150ml。

Facial mask of the present invention is made of following supplementary material: 0.5~4.0g of extractive of perilla, Rhizoma Polygonati extract 0.5~ 4.0g, III 1.0~2.0g of methylcellulose, 1.0~3.0g of sodium alginate, 4.0~10g of glycerol, rilanit special 1.0~ 3.0g, 1~3ml of oiliness ammonia ketone, 1~3ml of Tween-80, ethylparaben 0.2g, distilled water 150ml.

Preferably, facial mask of the present invention is made of following supplementary material: extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g, III 1.5g of methylcellulose, sodium alginate 3.0g, glycerol 7.0g, rilanit special 2.0g, 1~3ml of oiliness ammonia ketone, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml.

Preferably, facial mask of the present invention is made of following supplementary material: extractive of perilla 1.5g, Rhizoma Polygonati extract 1.5g, III 1.5g of methylcellulose, sodium alginate 3.0g, glycerol 7.0g, rilanit special 2.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml.

Preferably, facial mask of the present invention is made of following supplementary material: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 1.5g of methylcellulose, sodium alginate 3.0g, glycerol 7.0g, rilanit special 2.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml.

120ml distilled water is added the preparation method comprises the following steps: weigh methylcellulose III, sodium alginate in facial mask of the present invention, It is dissolved by heating in 95~102 DEG C of magnetic stirring apparatus；It takes glycerol and rilanit special to be placed in a beaker, stirs evenly, stand overnight Defoaming, obtains bare substrate；Extractive of perilla and Rhizoma Polygonati extract is added, stirs evenly, oiliness azone and tween, stirring is added Uniformly, with distilled water constant volume to 150mg, gel mask of the invention can be obtained.

Preferably, the preparation method comprises the following steps: weighing methylcellulose III, sodium alginate, addition 120ml steams facial mask of the present invention Distilled water dissolves by heating in 98 DEG C of magnetic stirring apparatus；It takes glycerol and rilanit special to be placed in a beaker, stirs evenly, placed Night defoaming, obtains bare substrate；Extractive of perilla and Rhizoma Polygonati extract is added, stirs evenly, oiliness azone and tween is added, stirs It mixes uniformly, with distilled water constant volume to 150mg, gel mask of the invention can be obtained.

Extractive of perilla source of the present invention are as follows: the purple perilla of integration of drinking and medicinal herbs product 99% obtained by being extracted as perilla leaf Original powder is purchased from Sen Fu QHC producer.

Rhizoma Polygonati extract source of the present invention are as follows: the rhizoma polygonati of integration of drinking and medicinal herbs product 98% obtained by being extracted as rhizoma polygonati Powder is purchased from Sen Fu QHC producer.

Facial mask of the present invention is in moisturizing and the application of anti-oxidant aspect.

The invention has the following advantages:

1, the defect and beneficial effects of the present invention of the prior art

The content that Chinese Patent Application No. CN201810380368.3 specification is recorded are as follows: the invention is organic nuisanceless, energy The effective moisturizing effect for improving facial mask, promotes the maintainability of facial mask.But it has the following deficiencies: 1. without anti-oxidation function；② Only facial mask additive；It 3. being not gel mask, therefore cannot mutually be depended on affected part for a long time, not can increase effect accordingly The Transdermal absorption amount of substance, drug effect is impacted, and stability is also poor；4. ingredient is more, higher cost；5. specific system is not known Preparation Method, curative effect are indefinite.

The present invention can overcome the defect of CN201810380368.3,1. having the advantages that has preferable antioxygen Change function, be 2. gel mask, can mutually be depended on affected part for a long time, can increase the Transdermal absorption amount of effect substance accordingly, imitated Fruit is good, and stability is preferable；3. ingredient is less, cost is relatively low；4. specifying specific preparation method, the product under this preparation method It is significant in efficacy.

2, it is shown by the experimental result of the measurement to [DPPH-] free radical scavenging activity, different pharmaceutical extract concentrations are multiple Fang Zisu gel mask is in certain dose relationship to the clearance rate of DPPH free radical and its concentration, and as drug extract is dense The increase of degree, clearance rate are gradually increased.Compared to the blank group, compound perilla gel mask (present invention) has preferable anti-oxidant Ability, compared with comparative example, oxidation resistance of the invention is better than comparative example.

3, by apple oxidation experiment, compound perilla gel mask easy cleaning does not dye, fades, visually observes it is found that right It is weaker than the moisturizing water lock ability of column, by experiment it is found that using the time it is longer when, just it is anti-oxidant with senile-resistant efficacy for, Compound perilla gel mask is better than comparative example.

4, it is shown by external moisturizing experimental result, moisturizing rate of the present invention has been up to 76.4%, is better than comparative example.

5, by estimation of stability the results show that sample pH value of the invention is 7, suitable skin is used, the heat-resisting patience of facial mask It can stablize；For centrefuge experiment without lamination, facial mask meets " Chinese Pharmacopoeia " (2015) version to the regulation of gel mask stability.

6, it is shown by skin irritation test result, product of the present invention is coated in three on healthy human body left finesse inside skin It is secondary not generate any bad phenomenon, it was demonstrated that the compound gel facial mask is nonirritant to healthy skin.

Detailed description of the invention:

Fig. 1: drug extract

Fig. 2: the choice experiment of extractive of perilla solvent

Fig. 3: the choice experiment of Rhizoma Polygonati extract solvent

Fig. 4: the choice experiment of coptis extract solvent

Fig. 5: DPPH clearance rate result column diagram

Fig. 6: orthogonal experiment (1. A₃、B₂、C₂、D₁, it may be assumed that III sodium 2.0g of methylcellulose, sodium alginate 2.0g, glycerol 7.0g, rilanit special 1.0g；②A₂、B₃、C₃、D₁, it may be assumed that III sodium 1.5g of methylcellulose, sodium alginate 3.0g, glycerol 10g, Rilanit special 1.0g；③A₂、B₂、C₁、D₂, it may be assumed that III sodium 1.5g of methylcellulose, sodium alginate 2.0g, glycerol 4.0g, hydrogenation Castor oil 2.0g；④A₃、B₁、C₃、D₂, it may be assumed that III sodium 2.0g of methylcellulose, sodium alginate 1.0g, glycerol 10.0g, hydrogenated castor Oily 2.0g；⑤A₁、B₁、C₁、D₁, it may be assumed that III sodium 1.0g of methylcellulose, sodium alginate 1.0g, glycerol 4.0g, rilanit special 1.0g；⑥A₂、B₁、C₂、D₃, it may be assumed that III sodium 1.5g of methylcellulose, sodium alginate 1.0g, glycerol 7.0g, rilanit special 3.0g； ⑦A₁、B₂、C₃、D₃, it may be assumed that III sodium 1.0g of methylcellulose, sodium alginate 2.0g, glycerol 10.0g, rilanit special 3.0g；⑧A₃、 B₃、C₁、D₃, it may be assumed that III sodium 2.0g of methylcellulose, sodium alginate 3.0g, glycerol 4.0g, rilanit special 3.0g；⑨A₂、B₃、C₂、 D₂, it may be assumed that III sodium 1.5g of methylcellulose, sodium alginate 3.0g, glycerol 7.0g, rilanit special 2.0g)

Fig. 7: (wherein No. 1 is bare substrate: Methyl cellulose to the screening experiment for preparing of optimal prescription compound perilla gel mask III sodium 1.5g of element, sodium alginate 3.0g, glycerol 7.0g, rilanit special 2.0g, distilled water 150ml；No. 2 are compound perilla gel Facial mask high concentration: III sodium 1.5g of methylcellulose, sodium alginate 3.0g, glycerol 7.0g, rilanit special 2.0g, distilled water 150ml, extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g；No. 3 are concentration in compound perilla gel mask: methylcellulose III Sodium 1.5g, sodium alginate 3.0g, glycerol 7.0g, rilanit special 2.0g, distilled water 150ml, extractive of perilla 1.5g, rhizoma polygonati mention Take object 1.5g；No. 4 are compound perilla gel mask low concentration: III sodium 1.5g of methylcellulose, sodium alginate 3.0g, glycerol 7.0g, rilanit special 2.0g, distilled water 150ml, extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g)

The high, medium and low concentration of Fig. 8 compound perilla gel mask, measurement of the comparative example to DPPH clearance rate

The external moisturizing experiment of Fig. 9

Figure 10 centrefuge experiment (after centrifugation)

Figure 11 high temperature resistant, low temperature test (restoring to room temperature)

The test of Figure 12 skin irritation

Before Figure 13 apple oxidation experiment 6h

After Figure 14 apple oxidation experiment 6h

Specific embodiment

The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..

Example 1 group side: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 1.0g of methylcellulose, sodium alginate 1.0g, glycerol 4.0g, rilanit special 1.0g, oiliness ammonia ketone 1ml, Tween-80 1ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: weighing methylcellulose III, sodium alginate, 120ml distilled water is added, in 95 DEG C of magnetic stirring apparatus It dissolves by heating；It takes glycerol and rilanit special to be placed in a beaker, stirs evenly, stand overnight defoaming, obtain bare substrate；It is added Extractive of perilla and Rhizoma Polygonati extract, stir evenly, and oiliness azone and tween is added, stirs evenly, is arrived with distilled water constant volume Gel mask of the invention can be obtained in 150mg.

2 prescription of embodiment: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 1.0g of methylcellulose, sodium alginate 1.0g, glycerol 4.0g, rilanit special 1.0g, oiliness ammonia ketone 1ml, Tween-80 1ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: weighing methylcellulose III, sodium alginate, 120ml distilled water is added, in 102 DEG C of magnetic stirring apparatus Middle heating for dissolving；It takes glycerol and rilanit special to be placed in a beaker, stirs evenly, stand overnight defoaming, obtain bare substrate；Add Enter extractive of perilla and Rhizoma Polygonati extract, stir evenly, oiliness azone and tween is added, stirs evenly, is arrived with distilled water constant volume Gel mask of the invention can be obtained in 150mg.

3 prescription of embodiment: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 1.0g of methylcellulose, sodium alginate 1.0g, glycerol 4.0g, rilanit special 1.0g, oiliness ammonia ketone 1ml, Tween-80 1ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: weighing methylcellulose III, sodium alginate, 120ml distilled water is added, in 98 DEG C of magnetic stirring apparatus It dissolves by heating；It takes glycerol and rilanit special to be placed in a beaker, stirs evenly, stand overnight defoaming, obtain bare substrate；It is added Extractive of perilla and Rhizoma Polygonati extract, stir evenly, and oiliness azone and tween is added, stirs evenly, is arrived with distilled water constant volume Gel mask of the invention can be obtained in 150mg.

4 prescription of embodiment: extractive of perilla 4.0g, Rhizoma Polygonati extract 4.0g, III 2.0g of methylcellulose, sodium alginate 3.0g, glycerol 10g, rilanit special 3.0g, oiliness ammonia ketone 3ml, Tween-80 3ml, ethylparaben 0.2g, distilled water 150ml.Preparation method: weighing methylcellulose III, sodium alginate, and 120ml distilled water is added, and adds in 95 DEG C of magnetic stirring apparatus Heat of solution；It takes glycerol and rilanit special to be placed in a beaker, stirs evenly, stand overnight defoaming, obtain bare substrate；It is added purple Soviet Union's extract and Rhizoma Polygonati extract, stir evenly, and oiliness azone and tween is added, stirs evenly, is arrived with distilled water constant volume Gel mask of the invention can be obtained in 150mg.

5 prescription of embodiment: extractive of perilla 4.0g, Rhizoma Polygonati extract 4.0g, III 2.0g of methylcellulose, sodium alginate 3.0g, glycerol 10g, rilanit special 3.0g, oiliness ammonia ketone 3ml, Tween-80 3ml, ethylparaben 0.2g, distilled water 150ml。

6 prescription of embodiment: extractive of perilla 4.0g, Rhizoma Polygonati extract 4.0g, III 2.0g of methylcellulose, sodium alginate 3.0g, glycerol 10g, rilanit special 3.0g, oiliness ammonia ketone 3ml, Tween-80 3ml, ethylparaben 0.2g, distilled water 150ml。

7 prescription of embodiment: extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g, III 1.5g of methylcellulose, sodium alginate 3.0g, glycerol 7.0g, rilanit special 2.0g, 1~3ml of oiliness ammonia ketone, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

8 prescription of embodiment: extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g, III 1.5g of methylcellulose, sodium alginate 3.0g, glycerol 7.0g, rilanit special 2.0g, 1~3ml of oiliness ammonia ketone, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Embodiment 9 (high concentration) prescription: extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g, III 1.5g of methylcellulose, Sodium alginate 3.0g, glycerol 7.0g, rilanit special 2.0g, 1~3ml of oiliness ammonia ketone, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml.

10 prescription of embodiment: extractive of perilla 1.5g, Rhizoma Polygonati extract 1.5g, III 1.5g of methylcellulose, sodium alginate 3.0g, glycerol 7.0g, rilanit special 2.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

11 prescription of embodiment: extractive of perilla 1.5g, Rhizoma Polygonati extract 1.5g, III 1.5g of methylcellulose, sodium alginate 3.0g, glycerol 7.0g, rilanit special 2.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Embodiment 12 (middle concentration) prescription: extractive of perilla 1.5g, Rhizoma Polygonati extract 1.5g, III 1.5g of methylcellulose, Sodium alginate 3.0g, glycerol 7.0g, rilanit special 2.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, Distilled water 150ml.

13 prescription of embodiment: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 1.5g of methylcellulose, sodium alginate 3.0g, glycerol 7.0g, rilanit special 2.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

14 prescription of embodiment: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 1.5g of methylcellulose, sodium alginate 3.0g, glycerol 7.0g, rilanit special 2.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Embodiment 15 (low concentration) prescription: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 1.5g of methylcellulose, Sodium alginate 3.0g, glycerol 7.0g, rilanit special 2.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, Distilled water 150ml.

16 prescription of embodiment: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 2.0g of methylcellulose, sodium alginate 2.0g, glycerol 7.0g, rilanit special 1.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

17 prescription of embodiment: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 2.0g of methylcellulose, sodium alginate 2.0g, glycerol 7.0g, rilanit special 1.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

18 prescription of embodiment: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 2.0g of methylcellulose, sodium alginate 2.0g, glycerol 7.0g, rilanit special 1.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

19 prescription of embodiment: extractive of perilla 1.5g, Rhizoma Polygonati extract 1.5g, III 2.0g of methylcellulose, sodium alginate 2.0g, glycerol 7.0g, rilanit special 1.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 16

20 prescription of embodiment: extractive of perilla 1.5g, Rhizoma Polygonati extract 1.5g, III 2.0g of methylcellulose, sodium alginate 2.0g, glycerol 7.0g, rilanit special 1.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 17

21 prescription of embodiment: extractive of perilla 1.5g, Rhizoma Polygonati extract 1.5g, methylcellulose III 2.0, sodium alginate 2.0g, glycerol 7.0g, rilanit special 1.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 18

22 prescription of embodiment: extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g, III 2.0g of methylcellulose, sodium alginate 2.0g, glycerol 7.0g, rilanit special 1.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 16

23 prescription of embodiment: extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g, III 2.0g of methylcellulose, sodium alginate 2.0g, glycerol 7.0g, rilanit special 1.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 17

24 prescription of embodiment: extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g, III 2.0g of methylcellulose, sodium alginate 2.0g, glycerol 7.0g, rilanit special 1.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 18

25 prescription of embodiment: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 1.5g of methylcellulose, sodium alginate 3.0g, glycerol 10.0g, rilanit special 1.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 16

26 prescription of embodiment: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 1.5g of methylcellulose, sodium alginate 3.0g, glycerol 10.0g, rilanit special 1.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 17

27 prescription of embodiment: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 1.5g of methylcellulose, sodium alginate 3.0g, glycerol 10.0g, rilanit special 1.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 18

28 prescription of embodiment: extractive of perilla 1.5g, Rhizoma Polygonati extract 1.5g, III 1.5g of methylcellulose, sodium alginate 3.0g, glycerol 10.0g, rilanit special 1.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 16

29 prescription of embodiment: extractive of perilla 1.5g, Rhizoma Polygonati extract 1.5g, III 1.5g of methylcellulose, sodium alginate 3.0g, glycerol 10.0g, rilanit special 1.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 17

30 prescription of embodiment: extractive of perilla 1.5g, Rhizoma Polygonati extract 1.5g, III 1.5g of methylcellulose, sodium alginate 3.0g, glycerol 10.0g, rilanit special 1.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 18

31 prescription of embodiment: extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g, III 1.5g of methylcellulose, sodium alginate 3.0g, glycerol 10.0g, rilanit special 1.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 16

32 prescription of embodiment: extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g, III 1.5g of methylcellulose, sodium alginate 3.0g, glycerol 10.0g, rilanit special 1.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 17

33 prescription of embodiment: extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g, III 1.5g of methylcellulose, sodium alginate 3.0g, glycerol 10.0g, rilanit special 1.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 18

34 prescription of embodiment: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 1.5g of methylcellulose, sodium alginate 2.0g, glycerol 4.0g, rilanit special 2.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 16

35 prescription of embodiment: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 1.5g of methylcellulose, sodium alginate 2.0g, glycerol 4.0g, rilanit special 2.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 17

36 prescription of embodiment: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 1.5g of methylcellulose, sodium alginate 2.0g, glycerol 4.0g, rilanit special 2.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 18

37 prescription of embodiment: extractive of perilla 1.5g, Rhizoma Polygonati extract 1.5g, III 1.5g of methylcellulose, sodium alginate 2.0g, glycerol 4.0g, rilanit special 2.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 16

38 prescription of embodiment: extractive of perilla 1.5g, Rhizoma Polygonati extract 1.5g, III 1.5g of methylcellulose, sodium alginate 2.0g, glycerol 4.0g, rilanit special 2.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 17

39 prescription of embodiment: extractive of perilla 1.5g, Rhizoma Polygonati extract 1.5g, III 1.5g of methylcellulose, sodium alginate 2.0g, glycerol 4.0g, rilanit special 2.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 18

40 prescription of embodiment: extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g, III 1.5g of methylcellulose, sodium alginate 2.0g, glycerol 4.0g, rilanit special 2.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 16

41 prescription of embodiment: extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g, III 1.5g of methylcellulose, sodium alginate 2.0g, glycerol 4.0g, rilanit special 2.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 17

42 prescription of embodiment: extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g, III 1.5g of methylcellulose, sodium alginate 2.0g, glycerol 4.0g, rilanit special 2.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 18

43 prescription of embodiment: extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g, III 2.0g of methylcellulose, sodium alginate 1.0g, glycerol 10.0g, rilanit special 2.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 16

44 prescription of embodiment: extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g, III 2.0g of methylcellulose, sodium alginate 1.0g, glycerol 10.0g, rilanit special 2.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 17

45 prescription of embodiment: extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g, III 2.0g of methylcellulose, sodium alginate 1.0g, glycerol 10.0g, rilanit special 2.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 18

46 prescription of embodiment: extractive of perilla 1.5g, Rhizoma Polygonati extract 1.5g, III 2.0g of methylcellulose, sodium alginate 1.0g, glycerol 10.0g, rilanit special 2.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 16

47 prescription of embodiment: extractive of perilla 1.5g, Rhizoma Polygonati extract 1.5g, III 2.0g of methylcellulose, sodium alginate 1.0g, glycerol 10.0g, rilanit special 2.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 17

48 prescription of embodiment: extractive of perilla 1.5g, Rhizoma Polygonati extract 1.5g, III 2.0g of methylcellulose, sodium alginate 1.0g, glycerol 10.0g, rilanit special 2.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 18

49 prescription of embodiment: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 2.0g of methylcellulose, sodium alginate 1.0g, glycerol 10.0g, rilanit special 2.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 16

50 prescription of embodiment: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 2.0g of methylcellulose, sodium alginate 1.0g, glycerol 10.0g, rilanit special 2.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 17

51 prescription of embodiment: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 2.0g of methylcellulose, sodium alginate 1.0g, glycerol 10.0g, rilanit special 2.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 18

52 prescription of embodiment: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 1.0g of methylcellulose, sodium alginate 1.0g, glycerol 4.0g, rilanit special 1.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 16

53 prescription of embodiment: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 1.0g of methylcellulose, sodium alginate 1.0g, glycerol 4.0g, rilanit special 1.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 17

54 prescription of embodiment: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 1.0g of methylcellulose, sodium alginate 1.0g, glycerol 4.0g, rilanit special 1.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 18

55 prescription of embodiment: extractive of perilla 1.5g, Rhizoma Polygonati extract 1.5g, III 1.0g of methylcellulose, sodium alginate 1.0g, glycerol 4.0g, rilanit special 1.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 16

56 prescription of embodiment: extractive of perilla 1.5g, Rhizoma Polygonati extract 1.5g, III 1.0g of methylcellulose, sodium alginate 1.0g, glycerol 4.0g, rilanit special 1.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 17

57 prescription of embodiment: extractive of perilla 1.5g, Rhizoma Polygonati extract 1.5g, III 1.0g of methylcellulose, sodium alginate 1.0g, glycerol 4.0g, rilanit special 1.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 18

58 prescription of embodiment: extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g, III 1.0g of methylcellulose, sodium alginate 1.0g, glycerol 4.0g, rilanit special 1.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 16

59 prescription of embodiment: extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g, III 1.0g of methylcellulose, sodium alginate 1.0g, glycerol 4.0g, rilanit special 1.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 17

60 prescription of embodiment: extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g, III 1.0g of methylcellulose, sodium alginate 1.0g, glycerol 4.0g, rilanit special 1.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 18

61 prescription of embodiment: extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g, III 1.5g of methylcellulose, sodium alginate 1.0g, glycerol 7.0g, rilanit special 3.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 16

62 prescription of embodiment: extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g, III 1.5g of methylcellulose, sodium alginate 1.0g, glycerol 7.0g, rilanit special 3.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 17

63 prescription of embodiment: extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g, III 1.5g of methylcellulose, sodium alginate 1.0g, glycerol 7.0g, rilanit special 3.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 18

64 prescription of embodiment: extractive of perilla 1.5g, Rhizoma Polygonati extract 1.5g, III 1.5g of methylcellulose, sodium alginate 1.0g, glycerol 7.0g, rilanit special 3.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 16

65 prescription of embodiment: extractive of perilla 1.5g, Rhizoma Polygonati extract 1.5g, III 1.5g of methylcellulose, sodium alginate 1.0g, glycerol 7.0g, rilanit special 3.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 17

66 prescription of embodiment: extractive of perilla 1.5g, Rhizoma Polygonati extract 1.5g, III 1.5g of methylcellulose, sodium alginate 1.0g, glycerol 7.0g, rilanit special 3.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 18

67 prescription of embodiment: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 1.5g of methylcellulose, sodium alginate 1.0g, glycerol 7.0g, rilanit special 3.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 16

68 prescription of embodiment: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 1.5g of methylcellulose, sodium alginate 1.0g, glycerol 7.0g, rilanit special 3.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 17

69 prescription of embodiment: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 1.5g of methylcellulose, sodium alginate 1.0g, glycerol 7.0g, rilanit special 3.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 18

70 prescription of embodiment: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 1.0g of methylcellulose, sodium alginate 2.0g, glycerol 10.0g, rilanit special 3.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 16

71 prescription of embodiment: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 1.0g of methylcellulose, sodium alginate 2.0g, glycerol 10.0g, rilanit special 3.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 17

72 prescription of embodiment: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 1.0g of methylcellulose, sodium alginate 2.0g, glycerol 10.0g, rilanit special 3.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 18

73 prescription of embodiment: extractive of perilla 1.5g, Rhizoma Polygonati extract 1.5g, III 1.0g of methylcellulose, sodium alginate 2.0g, glycerol 10.0g, rilanit special 3.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 16

74 prescription of embodiment: extractive of perilla 1.5g, Rhizoma Polygonati extract 1.5g, III 1.0g of methylcellulose, sodium alginate 2.0g, glycerol 10.0g, rilanit special 3.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 17

75 prescription of embodiment: extractive of perilla 1.5g, Rhizoma Polygonati extract 1.5g, III 1.0g of methylcellulose, sodium alginate 2.0g, glycerol 10.0g, rilanit special 3.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 18

76 prescription of embodiment: extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g, III 1.0g of methylcellulose, sodium alginate 2.0g, glycerol 10.0g, rilanit special 3.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 16

77 prescription of embodiment: extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g, III 1.0g of methylcellulose, sodium alginate 2.0g, glycerol 10.0g, rilanit special 3.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 17

78 prescription of embodiment: extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g, III 1.0g of methylcellulose, sodium alginate 2.0g, glycerol 10.0g, rilanit special 3.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 18

79 prescription of embodiment: extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g, III 2.0g of methylcellulose, sodium alginate 3.0g, glycerol 4.0g, rilanit special 3.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 16

80 prescription of embodiment: extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g, III 2.0g of methylcellulose, sodium alginate 3.0g, glycerol 4.0g, rilanit special 3.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 17

81 prescription of embodiment: extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g, III 2.0g of methylcellulose, sodium alginate 3.0g, glycerol 4.0g, rilanit special 3.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 18

82 prescription of embodiment: extractive of perilla 1.5g, Rhizoma Polygonati extract 1.5g, III 2.0g of methylcellulose, sodium alginate 3.0g, glycerol 4.0g, rilanit special 3.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 16

83 prescription of embodiment: extractive of perilla 1.5g, Rhizoma Polygonati extract 1.5g, III 2.0g of methylcellulose, sodium alginate 3.0g, glycerol 4.0g, rilanit special 3.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 17

84 prescription of embodiment: extractive of perilla 1.5g, Rhizoma Polygonati extract 1.5g, III 2.0g of methylcellulose, sodium alginate 3.0g, glycerol 4.0g, rilanit special 3.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 18

85 prescription of embodiment: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 2.0g of methylcellulose, sodium alginate 3.0g, glycerol 4.0g, rilanit special 3.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 16

86 prescription of embodiment: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 2.0g of methylcellulose, sodium alginate 3.0g, glycerol 4.0g, rilanit special 3.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 17

87 prescription of embodiment: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 2.0g of methylcellulose, sodium alginate 3.0g, glycerol 4.0g, rilanit special 3.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml。

Preparation method: with embodiment 18

88 prescription of embodiment: extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g.

89 prescription of embodiment: extractive of perilla 4.0g, Rhizoma Polygonati extract 4.0g.

90 prescription of embodiment: extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g.

The method that method 1-4 is prepared as follows in the prescription of embodiment 88-90 is prepared into corresponding dosage form.

The drug of formula ratio: being added 1/10 dextrin, granulation by preparation method 1, and tablet is made in tabletting.

Preparation method 2: the drug of formula ratio is added to 1/12 dextrin, pelletizes according to a conventional method, obtains granule.

Preparation method 3: the drug of formula ratio is added to 1/10 dextrin, capsule is made according to a conventional method, obtains capsule.

Preparation method 4: taking the dextrin of the drug addition 1/8 of formula ratio to be uniformly mixed, dry, and pill is made.

In order to further verify feasibility of the invention, inventor has carried out a series of experiment, specific as follows:

1 laboratory apparatus, medicinal material and reagent

1.1 laboratory apparatus

Constant Temp. Oven (HPD-101-3A, Changzhou HuaPu reach instruments used for education Co., Ltd), household refrigerator-freezer (BCD-118TMPA, Qingdao HaiEr Co., Ltd), (SK8-210-HP, Shanghai section leads ultrasonic instrument to be had ultrasonic cleaner Limit company), digital display thermostat water bath (DRRH-S4, Shanghai Shuan Jie experimental facilities Co., Ltd), electronic balance (FA2-204-N, Shanghai Jing Hai Instrument Ltd.), heat-collecting magnetic stirring device (DF-101S, Jiangsu Ke Xi Instrument Ltd.), digital display rotation Viscosimeter (NDJ-9S, Shanghai Ping Xuan scientific instrument Co., Ltd), PH count tester (PHS-25, Shanghai instrument electricity scientific instrument stock Part Co., Ltd), desktop electric centrifuge (80-2, Changzhou Jintan beneficial friend Instrument Ltd.), ultraviolet-visible photometer (Shanghai UV-5900 Yuan Xi Instrument Ltd.).

1.2 medicinal substances extracts and reagent

Extractive of perilla (see Fig. 1-2), Rhizoma Polygonati extract (see Fig. 1-1), coptis extract (see Fig. 1-3), five oxidations two Phosphorus, -2 picryl hydrazine of 1,1- diphenyl (Shanghai DPPH Jin Sui Biotechnology Co., Ltd), sodium alginate (Sodium alginate, SA, Sinopharm Chemical Reagent Co., Ltd.), carboxymethyl cellulose III (Carboxymethyl cellulose III, Aladdin Industrial Co., USA), glycerine (Glycerol, Chongqing Chuan Dong Chemical Co., Ltd.), medical grade oiliness Azone (Azone, Tian Hong Bioisystech Co., Ltd, Zhejiang Province), Tween-80 (Tween-80, Aladdin Industrial Co., USA), rilanit special (PEG-60 triumphant code biochemistry Tianjin Co., Ltd), ethylparaben (Ethyl p- Hydrobenzoate, Sa En chemical technology Co., Ltd), it is that analysis is pure, and experimental water is that this, which tests other agents useful for same, Pure water.

2 experimental methods and result

The screening of 2.1 bare substrates

To make drug be substantially soluble in mask matrix, take carboxymethyl cellulose III, sodium alginate and polyvinyl alcohol each respectively 1.0g, respectively plus 100mL distilled water, stirring a moment, 100 DEG C of heating water bath 30min are parallel to add after matrix is sufficiently swollen or dissolves Enter same drug, stirring is dissolved to drug is visually observed, the first one-step forming of gel mask.When necessary can ultrasound 5min, stand 2h after It visually observes.And respectively taking 6mL facial mask in 4 10mL plastics small test tubes, 5000r/min is centrifuged 30min.According to the scoring mark of table 7 Standard carries out comprehensive score, comprehensive score=indices score summation, and appraisal result is shown in Table 1.

1 bare substrate the selection result of table

Data are shown in table, and the comprehensive score highest of carboxymethyl cellulose III, sodium alginate takes second place.Carboxymethyl cellulose III It is not much different with sodium alginate comprehensive score, and unchanged after gel mask centrifugation, thus carboxymethyl cellulose III and alginic acid Sodium can allow dispersion of medicine as drug matrices, and without agglomeration or precipitating after centrifugation, property is stablized.

The preparation of 2.2 gel masks

2.2.1 the selection of drug extract solvent

2..2.1.1 the selection of extractive of perilla solvent

Distilled water, 65% ethyl alcohol, 75% ethyl alcohol, each 5mL of 95% ethyl alcohol are measured respectively, and 0.5g extractive of perilla is added, stirs It mixes 5 minutes.Observe the dissolution situation of extractive of perilla.

As a result: extractive of perilla is completely dissolved water, has precipitating in other solvents.So selecting water as purple perilla The solvent of extract.See Fig. 2.

2.2.1.2 the selection of Rhizoma Polygonati extract solvent

Distilled water, 65% ethyl alcohol, 75% ethyl alcohol, each 5mL of 95% ethyl alcohol are measured respectively, and 0.5g Rhizoma Polygonati extract is added, stirs It mixes 5 minutes.Observe the dissolution situation of Rhizoma Polygonati extract.

As a result: Rhizoma Polygonati extract is completely dissolved water, has precipitating in other solvents.So selecting water as rhizoma polygonati The solvent of extract.See Fig. 3.

2.2.1.3 the selection of coptis extract solvent

Distilled water, 65% ethyl alcohol, 75% ethyl alcohol, each 5mL of 95% ethyl alcohol are measured respectively, and 0.5g coptis extract is added, stirs It mixes 5 minutes.Observe the dissolution situation of coptis extract.

As a result: coptis extract is completely dissolved water, has precipitating in other solvents.So selecting water as the coptis The solvent of extract.See Fig. 4.

In view of most of organic solvents are harmful to the human body, the safety of ethyl alcohol is relatively high, so this experiment only selects The ethyl alcohol of distilled water and various concentration carries out the selection of solvent.

2.2.2 the selection of the first prescription of purple perilla gel mask

Preliminary design five prescriptions, respectively (1): folk prescription purple perilla；(2): folk prescription rhizoma polygonati: (3) purple perilla: rhizoma polygonati (1:1)； (4) purple perilla: rhizoma polygonati: the coptis (1:1:0.5)；(5) purple perilla: rhizoma polygonati: the coptis (2:1:0.5).Each prescription 6.0g is taken respectively, is resisted Oxidisability experiment.It the results are shown in Table 2,3,4,5, Fig. 5.

Anti-oxidant experiment: taking each prescription Aqueous extracts 0.5ml respectively, is separately added into 4mlDPPH solution, stands 30min, Its absorbance value is measured under 517nm, is measured in parallel three times.

Clearance rate %=[A control-(A sample-A blank)]/A control × 100%

In formula: A control is the absorbance of DPPH reaction system；A sample is the absorbance that the DPPH of sample is added；A blank For the absorbance of sample itself.

Absorbance/A the control at 517nm of 2 DPPH solution of table

3 blank sample of table absorbance/A blank at 517nm

4 sample+DPPH of table absorbance/A control at 517nm

5 extracting solution clearance rate result of table

Table 5 and Fig. 5 data are shown, compared with bare substrate, the clearance rate of each prescription is all higher than bare substrate, wherein locating Side's (3) purple perilla: rhizoma polygonati (1:1) is maximum to DPPH free radical scavenging activity, so selecting purple perilla: rhizoma polygonati (1:1) is used as compound perilla The first prescription of facial mask.

It tentatively sums up according to the selection of prescription " 2.2.1 purple perilla gel mask just " result and after consulting relevant materials multiple Fang Zisu facial mask prescription, is specifically shown in Table 6.

The preliminary prescription of 6 compound perilla gel mask of table

2.2.3 the preparation of purple perilla gel mask

According to 6 prescription of table, III 2.0g of methylcellulose, sodium alginate 1.0g are weighed, the distillation of appropriate (120mL) is added Water dissolves by heating in 98 DEG C of magnetic stirring apparatus；It takes glycerol 7.0g and rilanit special 1.0g to be placed in a beaker, stirs evenly, Defoaming is stood overnight, bare substrate is obtained.Extractive of perilla 3.0g and Rhizoma Polygonati extract 3.0g is added, stirs evenly；Nitrogen is added Ketone and each 2mL of tween, stir evenly, with water constant volume to 150mg；Brown compound perilla facial mask can be obtained in mixing.

2.2.4 it scores purple perilla gel mask

According to 7 compound perilla gel mask standards of grading of table, comprehensive score made to the facial mask prepared, comprehensive score= The sum of indices, full marks 10 divide.

7 compound perilla gel mask matrix standards of grading of table

According to the standards of grading in table 7, the compound perilla facial mask just prepared from dissolubility, glossiness, Tu malleability and from Heart degree etc. carries out preliminary screening scoring, appraisal result is excellent (2.0-2.5), good (1.0-1.9), in (0.4-0.9) and Poor (0-0.3) four grades have comprehensive score and individual scores two to its marking mode due to respectively screening object difference in experiment Kind mode.Comprehensive score refers to score respectively from four dissolubility, glossiness, Tu malleability and centrifugation degree angles, and synthesis is commented Divide=indices score summation, full marks 10 divide；And individual scores refer to that four select one to score, full marks 2.5 divide.

The prescription of the preferred compound perilla facial mask of 2.3 experiment of single factor

2.3.1 the dosage of methylcellulose III

3 parts of gel masks, Methyl cellulose are prepared according to the preliminary prescription content of table 6 is parallel with compound perilla facial mask preparation method The dosage of element III takes 1.5g, 2.0g, 2.5g respectively, and other conditions are consistent.Comprehensive score is carried out according to standards of grading table 7, it is comprehensive Scoring=indices score summation, appraisal result are shown in Table 8.

8 compound perilla facial mask methylcellulose of table, III dosage appraisal result

The appraisal result from table it is found that III dosage of methylcellulose be 2.5g when comprehensive scores highest, and facial mask be in brown Semi-transparent gelatin-like, modest viscosity are in good condition.So primarily determining that III dosage of methylcellulose is that 2.5g is appropriate.

2.3.2 sodium alginate dosage is investigated

3 parts of gel masks, sodium alginate are prepared according to the preliminary prescription content of table 6 is parallel with compound perilla facial mask preparation method Dosage take 0.5g, 1.0g, 1.5g respectively, other conditions are consistent.Comprehensive score, comprehensive score are carried out according to standards of grading table 7 =indices score summation, appraisal result are shown in Table 9.

9 compound perilla facial mask sodium alginate dosage appraisal result of table

The appraisal result from table 9 it is found that sodium alginate dosage be 1.5g when comprehensive scores highest, and facial mask in brown it is semi-transparent Gelatin-like, modest viscosity are in good condition.So primarily determining that sodium alginate dosage is that 1.5g is appropriate.

2.3.3 the investigation of glycerol dosage

3 parts of gel masks, sodium alginate are prepared according to the preliminary prescription content of table 6 is parallel with compound perilla facial mask preparation method Dosage take 0.5g, 1.0g, 1.5g respectively, other conditions are consistent.It is evaluated using moisturizing rate.0.5g is taken to set 3M medical proof fabric On, it is placed in calcium chloride drier, it is 12 hours dry, measure moisturizing rate.It the results are shown in Table 10.

Moisturizing rate (100%)=M2/M1 × 100%

In formula: M2 is to place, the quality g of sample after 12h；M1 is the quality g of sample before placing.

The investigation result of 10 glycerol dosage of table

As can be seen from Table 10, moisturizing rate highest when glycerol dosage is 10.0g, so initial option glycerol dosage is 10.0g。

2.3.4 the investigation of rilanit special dosage

3 parts of gel masks, sodium alginate are prepared according to the preliminary prescription content of table 6 is parallel with compound perilla facial mask preparation method Dosage take 0.5g, 1.0g, 1.5g respectively, other conditions are consistent.Disappeared degree, the highly seasoned light degree of oil and exquisiteness using the difficulty of bubble Degree is evaluated.Rilanit special dosage investigates overall merit=bubble difficulty and disappears the highly seasoned light degree+exquisiteness of degree scoring+oil Degree.Each single item score value is 2.5, total score 7.5.It the results are shown in Table 11.

The investigation result of 11 rilanit special dosage of table

The appraisal result from table 11 it is found that rilanit special dosage be 1.0g when comprehensive scores highest, and facial mask be in brown Semi-transparent gelatin-like, modest viscosity are in good condition.So primarily determining that rilanit special dosage is that 1.0g is appropriate.

2.3.5 the investigation of ethylparaben dosage

Ethylparaben makees sterilization antiseptic, and dosage is to experimental result and has no significant effect, so in the normal range In view of cost problem, fixing tentatively ethylparaben is 0.2g.

2.3.6 the investigation of oiliness azone dosage

According to consulting reference materials it is found that azone effective concentration makees penetrating agent 1%~6%, fix tentatively oiliness azone dosage be 1~ 3ml。

2.3.7 the dosage of Tween-80

Tween-80 makees solubilizer, and dosage is to experimental result and has no significant effect, so considering in the normal range Cost problem, fixing tentatively 80 dosage of tween-is 1~3ml, preferably 2ml.

The prescription of the preferred compound perilla facial mask of 2.4 orthogonal experiments

According to experiment of single factor as a result, discovery methylcellulose III, sodium alginate, glycerol and rilanit special are to compound The superiority and inferiority of purple perilla facial mask shaping has a significant impact, thus by its four do it is orthogonal preferably, orthogonal design table is shown in Table 12.

12 factor of table and level

Using L₉(3⁴) orthogonal table, according to orthogonal design table experiment arrangement, with compound perilla facial mask at room temperature Solubility, glossiness, stretchability, centrifugation degree are index, and comprehensive score preferably goes out best prescription, and comprehensive score=indices obtain Point summation, experimental design group number and the results are shown in Table 13, Fig. 6, Fig. 7.

13 L9 (3 of table⁴) Orthogonal experiment results

It is very poor according to each factor R the results show that A > C > D > B, it is seen that factor A influences maximum, factor C times to experimental result It, factor B influences minimum；K value is the results show that best factor level is A respectively₂、B₃、C₂、D₂, other factors level is can Row scheme.It is III 1.5g of methylcellulose, sodium alginate 3g, glycerol 7g and rilanit special 2g up to matrix best prescription.

2.5 confirmatory experiment

Compound perilla facial mask best prescription proportion --- the i.e. A preferably gone out in orthogonal experiment₂B₃C₂D₂, by its parallel preparation 3 Part, comprehensive score, comprehensive score=indices score summation are carried out according to 7 standards of grading of table, appraisal result is shown in Table 14.

14 optimised process confirmatory experiment result of table

A as the result is shown₂B₃C₂D₂, --- i.e. III 1.5g of methylcellulose, sodium alginate 3g, glycerol 7g and hydrogenated castor Oily 2g is the best prescription of compound perilla facial mask, and facial mask is in the semi-transparent gelatin-like of brown, and apparent condition is good.

3 parts of each 150mL of compound perilla facial mask are prepared under optimizing prescriptions in parallel, 3 parts have 450ml altogether, do quality evaluation experiment Mother liquor.

2.6 compound perilla facial masks, the anti-oxidant experiment of comparative example

2.6.1 [DPPH-] radicals scavenging is tested

(1) experimental principle: diphenyl bitter taste diazanyl free radical [DPPH-] is a kind of stable freedom centered on nitrogen Base has absorption maximum at 517nm wavelength.[DPPH-] ethyl alcohol or methanol solution are purple, and concentration is linearly closed with absorbance System.

It is added in [DPPH-] ethanol solution after there is oxidation resistant preferred facial mask extracting solution, facial mask can be with [DPPH-] Free radical is combined or is substituted, and reduces [DPPH-] free radical quantity, and solution colour shoals, and shows themselves in that it in 517nm wave Strong point detection, to the clear effect of [DPPH-] free radical, is calculated its oxidation resistance by test product.

(2) DPPH-ethanol solution preparation

4.7mgDPPH is accurately weighed, with dehydrated alcohol dissolution (ultrasonic dissolution 20min) and is settled to 10mL, sealing is protected from light It saves (0~4 DEG C), when use is diluted to 120umol/L, ready-to-use.

(3) senior middle school's low concentration gel mask extracting solution, measurement of the comparative example to [DPPH-] free radical scavenging activity

By preferred bare substrate and the present invention high (embodiment 9), in (embodiment 12), low (embodiment 15) three concentration, The product wiring solution-forming of comparative example (application number CN201810380368.3) is drawn 1mL sample in test tube, is added 120umol/L[DPPH-] ethanol solution, it sufficiently shakes up, absorbance is measured at 517nm wavelength, measure 30min, start to react Preceding 15min is surveyed once every 1min, primary every 5min measurement later, is measured in parallel three times.15 are shown in Table, is calculated according to the following formula Each sample is to [DPPH-] free radical scavenging activity:

Clearance rate/%=[A2- (A1-A3)]/A2*100%

In formula, the absorbance of A1:1mL facial mask solution+2mL [DPPH-] ethanol solution

The absorbance of A2:1mL distilled water+2mL [DPPH-] ethanol solution

The absorbance of A3:1mL facial mask solution+2mL ethanol solution

Blank control: 1mL distilled water+2mL dehydrated alcohol

Blank feminine gender/positive control: 1mL distilled water+2mL [DPPH-] ethanol solution

The high, medium and low concentration of 15 compound perilla gel mask of table, comparative example are to DPPH clearance rate measurement result

(4) drafting of [DPPH-] radicals scavenging curve

According to 15 measurement result of table with time (min) for abscissa, compound perilla facial mask is vertical to DPPH clearance rate (%) Coordinate makees DPPH free radical scavenging activity (%) curve such as Fig. 8:

By table 15 and Fig. 8 it is found that being tested by [DPPH-] radicals scavenging, different pharmaceutical extract concentrations compound perilla Gel mask is in certain dose relationship to the clearance rate of DPPH free radical and its concentration, and with the increasing of drug extract concentration Add, clearance rate is gradually increased.It is proved compared with blank, compound perilla gel mask has preferable oxidation resistance and its concentration Higher, oxidation resistance is better.The oxidation resistance of compound perilla gel mask known to simultaneously is substantially better than comparison column.

2.6.2 apple oxidation experiment

After apples peeling or incision, apple notch aoxidizes the phenomenon that becoming color and is called enzymatic browning, mainly in apple Polyphenol oxidase (PPO) phenols substrate oxidation be quinones, quinone polymerization become brown pigment and cause Tissue Browning, i.e., daily life Observable apple is oxidized phenomenon in work.

Enzymatic browning reaction mechanism: containing phenolic substances in plant tissue, as breathing transmitting object in complete cell Matter remains dynamic equilibrium in phenol-quinone, and after cell tissue is destroyed, oxygen just a large amount of intrusions cause the formation of quinone to go back with it Imbalance between original reaction, then has occurred the accumulation of quinone, and quinone further oxidation polymerization is formed brown pigment, claims For melanin or melanoidin.

The method that plant cell influences enzymatic browning has very much, but approach is mainly four following:

1. the activity for being passivated phenolase (adjusts temperature, inhibitor etc..)

2. changing the condition (pH value, water activity etc.) of phenolase effect

3. the contact of starvation

4. using antioxidant (ascorbic acid, SO2 etc.).

Due to this experimental study be compound perilla gel mask antioxygenic property, this experiment is by fresh apple Fruit is cut into the uniform slice of size, thickness, and the oxidization condition of apple is observed by the method to apple face mask film, with blank pair Photograph, which is compared, makes analysis, to obtain experimental result.

Experimental design is blank group: non-face mask film, natural exposing is in air；Bare substrate group: bare substrate is uniform It is coated on apple section；Embodiment 15 (low concentration group): the low concentration compound perilla gel mask of preparation is uniformly coated on apple Fruit section；Concentration group in (embodiment 12): the middle concentration compound perilla gel mask of preparation is uniformly coated on apple section； (embodiment 9) high concentration group: the high concentration compound perilla gel mask of preparation is uniformly coated on apple section；Compare column group: This experiment is using the ocean Po Laiya energy facial mask mud living as comparative example, and it was evenly coated on apple sections.Each test group is parallel Three parts of preparation is observed after placing 6h in air with clear water is clean.See Figure 13；

Experimental result is shown in Figure 14.

Blank group: it is deeper that naked eyes obviously observe that apple is oxidized degree, and entire slice becomes brown, and apple slice water Divide and tails off；

Bare substrate group: the clearly visible apple slice of naked eyes becomes brown, but still retains large quantity of moisture；

Low concentration group: apple slice still keeps the freshness of about 80%-95%；

Middle concentration group；Apple slice still keeps the freshness of about 80%-95%；

High concentration group: apple slice still keeps the freshness of about 80%-95%；

Comparative example group: easy cleaning, apple slice are not aoxidized comparative example slightly by slight stain, apple slice, and moisture has stream It loses.

Conclusion:

1. the apple slice of non-face mask film is easy dehydration, oxidation in the short time, becomes stale, it is brown to be easy to happen enzymatic Become；

2. the apple slice of the bare substrate without drug extract has been applied, it in a short time can be with the apple of non-face mask film Apparent enzymatic browning phenomenon equally occurs for slice；

3. the compound perilla gel mask of this experiment preparation has certain oxidation resistance；

4. in the short time, the compound perilla gel mask of basic, normal, high various concentration can make apple slice keep fresh, It is not oxidized；

5. compound perilla gel mask easy cleaning does not dye, fades compared with comparative example, slightly it is better than the antioxygen of comparative example The property changed visually observes it is found that the moisturizing water lock ability for comparing column is weaker.To sum up, using the time it is longer when, just it is anti-oxidant and anti-ageing For old effect, compound perilla gel mask is better than comparative example.

2.7 compound perilla facial mask (present invention) quality evaluations experiment

Parallel preparation embodiment 9, embodiment 12, the product of embodiment 15 be 3 parts each, every part of 100mL, does quality evaluation experiment Mother liquor.

2.7.1 appearance character and sophistication

Each 5g of finished product of Example 9, embodiment 12, embodiment 15, is placed in the test tube of 10mL, observes its appearance Character；It takes finished product appropriate, is coated on the back of the hand, its sophistication is evaluated by organoleptic indicator.

2.7.2 pH value

Each 2.5g of finished product of Example 9, embodiment 12, embodiment 15, is dissolved in 50mL distilled water, 80 DEG C of constant temperature 30min is cooled to room temperature, measures pH value.

2.7.3 viscosimetric analysis (providing to carry out according to 2015 editions " Chinese Pharmacopoeias " (four) general rule)

Parallel Example 9, embodiment 12, each 3 parts of product, every part of 100mg of embodiment 15, according to 2015 editions " China Pharmacopeia " 0633 method of (four) general rule, at room temperature using rotor-type rotational viscometer (No. 4 rotors, revolving speed 6.0r/min) measurement The viscosity of gel mask.

2.7.4 external moisturizing experiment

It selects in order to preferably simulate human skin, in experiment and to manufacture simulator in one layer of 3M medical air-permeable adhesive tape. Temperature is maintained at room temperature in experiment, weighs embodiment 9, embodiment 12, the product of embodiment 15 and comparative example (application number CN201810380368.3) each 0.5g facial mask is coated on simulator surface, places at room temperature, weighs after 12h, calculates moisturizing Rate.

Moisturizing rate (100%)=M2/M1 × 100%

In formula: M2 is to place, the quality g of sample after 12h；

M1 is the quality g of sample before placing.

2.7.5 high temperature resistant, low temperature test

Example 9, embodiment 12, embodiment 15 each 70g of product be fitted into centrifuge tube, be placed in 100 DEG C of thermostatted water In bath and -5, -10 DEG C of refrigerator, keep for 24 hours, restoring whether have notable difference before observing and test to room temperature.

2.7.6 centrefuge experiment

At room temperature, the product 70g of Example 15 is fitted into the centrifuge tube of 100mL, be placed in a centrifuge with 1000, 2000, the speed rotation 10min of 3000,4000,5000rpm, observation sample with or without moisture are precipitated.

2.7.7 skin irritation is tested

Parallel Example 9, embodiment 12, the product of embodiment 15 are each three parts (appropriate), are coated in a healthy human body left side respectively It on wrist inside skin, applies in three times, primary holding visually observes after 24 hours, 24 hours.

2.8 quality evaluation result

2.8.1 the appearance character and viscosity of compound perilla facial mask

This research embodiment 9, embodiment 12, embodiment 15 product be the semi-transparent gelatin-like of brown, uniform and smooth has light Pool, sense of touch lubrication and modest viscosity；And comparative example has a fine particle, matt, sense of touch is coarse and without viscosity.

2.8.2 external moisturizing experimental result, is shown in Table 16

The external moisturizing experimental result of table 16

Moisturizing evaluation result: table 16 shows that compound perilla gel mask (basic, normal, high concentration) prepared by the present invention has Excellent moisture retention, and better than comparison column.See Fig. 9.

2.8.3 the estimation of stability result of purple perilla compound facial mask

Obtained purple perilla compound facial mask is brown, is evenly distributed, is readily cleaned, without granular sensation, exquisiteness after smearing skin It is soft and smooth, salubrious wet.The indices for measuring purple perilla compound facial mask, are recorded in table 17.

According to 17 result of table and Figure 10,11 it is found that the pH of sample is 7, it is suitble to skin.The heat-resisting cold tolerance of facial mask is stablized； For centrefuge experiment without lamination, facial mask meets " Chinese Pharmacopoeia " (2015) version to the regulation of gel mask stability.

17 Evaluation results of table

2.8.4 skin irritation test result

Parallel Example 9, embodiment 12, the product of embodiment 15 are each three parts (appropriate), are coated in a healthy human body left side respectively On wrist inside skin, every part of product applies in three times, and primary holding visually observes after 24 hours, 24 hours.It does not generate three times Any bad phenomenon, the preliminary proof compound gel facial mask are nonirritant to healthy skin.Seeing Figure 12, (choosing figure is from embodiment 9 Product experiment).

2.8 research experiment conclusions

This research and development is probed into moisturizing antioxidant activity facial mask and preparation method, as the result is shown purple perilla: when rhizoma polygonati 1: 1, Its antioxidant activity is best, and the research of solubility, glossiness, stretchability, centrifugation degree has been carried out by orthogonal experiment, screening The proportion of optimal matrix and Chinese medical extract out, sodium carboxymethylcellulose 1.0g, sodium alginate 3.0g, glycerol 7.0mL, hydrogenation Castor oil 2.0g, extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g prepare a brown and are readily cleaned the face to work well Film.PH value is detected as 7, and has carried out irritation evaluation to product, and experiment indices meet the quality standard of facial mask.

Although above having used general explanation, specific embodiment and experiment, the present invention is made to retouch in detail State, but on the basis of the present invention, it can be made it is some modify or improve, this is aobvious and easy to those skilled in the art See.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to claimed Range.

Claims

1. a kind of anti-oxidant gel mask, which is characterized in that the facial mask is made of following raw material: extractive of perilla 0.5~ 4.0g, 0.5~4.0g of Rhizoma Polygonati extract.

2. anti-oxidant gel mask according to claim 1, which is characterized in that the facial mask further includes with can pharmaceutically connect Pharmaceutically acceptable dosage form is made in the auxiliary material received.

3. anti-oxidant gel mask according to claim 2, which is characterized in that the auxiliary material are as follows: methylcellulose III 1.0 ~2.0g, it 1.0~3.0g of sodium alginate, 4.0~10g of glycerol, 1.0~3.0g of rilanit special, 1~3ml of oiliness ammonia ketone, spits - 80 1~3ml of temperature, ethylparaben 0.2g, distilled water 150ml.

4. anti-oxidant gel mask according to claim 3, which is characterized in that the facial mask is made of following supplementary material: 0.5~4.0g of extractive of perilla, 0.5~4.0g of Rhizoma Polygonati extract, III 1.0~2.0g of methylcellulose, sodium alginate 1.0~ 3.0g, 4.0~10g of glycerol, 1.0~3.0g of rilanit special, 1~3ml of oiliness ammonia ketone, 1~3ml of Tween-80, nipalgin second Ester 0.2g, distilled water 150ml.

5. anti-oxidant gel mask according to claim 4, which is characterized in that the facial mask is made of following supplementary material: Extractive of perilla 3.0g, Rhizoma Polygonati extract 3.0g, III 1.5g of methylcellulose, sodium alginate 3.0g, glycerol 7.0g, hydrogenated castor Oily 2.0g, 1~3ml of oiliness ammonia ketone, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml.

6. anti-oxidant gel mask according to claim 4, which is characterized in that the facial mask is made of following supplementary material: Extractive of perilla 1.5g, Rhizoma Polygonati extract 1.5g, III 1.5g of methylcellulose, sodium alginate 3.0g, glycerol 7.0g, hydrogenated castor Oily 2.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml.

7. anti-oxidant gel mask according to claim 4, which is characterized in that the facial mask is made of following supplementary material: Extractive of perilla 0.5g, Rhizoma Polygonati extract 0.5g, III 1.5g of methylcellulose, sodium alginate 3.0g, glycerol 7.0g, hydrogenated castor Oily 2.0g, oiliness ammonia ketone 2ml, Tween-80 2ml, ethylparaben 0.2g, distilled water 150ml.

8. a kind of method for preparing any one of claim 1-7 facial mask, which is characterized in that the facial mask the preparation method comprises the following steps: Methylcellulose III, sodium alginate are weighed, 120ml distilled water is added, is dissolved by heating in 95~102 DEG C of magnetic stirring apparatus；It takes Glycerol and rilanit special are placed in a beaker, and are stirred evenly, and are stood overnight defoaming, are obtained bare substrate；Extractive of perilla is added It with Rhizoma Polygonati extract, stirs evenly, oiliness azone and tween is added, stirs evenly, with distilled water constant volume to 150mg, can obtain To gel mask of the invention.

9. gel mask preparation method according to claim 8, which is characterized in that the facial mask is the preparation method comprises the following steps: weigh Methylcellulose III, sodium alginate are added 120ml distilled water, dissolve by heating in 98 DEG C of magnetic stirring apparatus；Take glycerol and hydrogenation Castor oil is placed in a beaker, and is stirred evenly, and is stood overnight defoaming, is obtained bare substrate；Extractive of perilla is added and rhizoma polygonati extracts Object stirs evenly, and oiliness azone and tween is added, stirs evenly, with distilled water constant volume to 150mg, can be obtained of the invention Gel mask.

10. facial mask according to claim 1-8, it is characterised in that the facial mask is at moisturizing and anti-oxidant aspect Using.