CN110269068A - A kind of macleaya cordata aqueous suspension agent biological pesticide and preparation method thereof - Google Patents
A kind of macleaya cordata aqueous suspension agent biological pesticide and preparation method thereof Download PDFInfo
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- CN110269068A CN110269068A CN201910457479.4A CN201910457479A CN110269068A CN 110269068 A CN110269068 A CN 110269068A CN 201910457479 A CN201910457479 A CN 201910457479A CN 110269068 A CN110269068 A CN 110269068A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention belongs to pesticide production technology field, especially a kind of macleaya cordata aqueous suspension agent biological pesticide and preparation method thereof；Its component, by weight percentage, comprising: 99.7%-99.8% component a, 0.20-0.30% component b；The component a, by weight percentage, comprising: 1%-5% Macleaya cordata alkaloids, 5%-20% dispersing agent, 0.5%-5% thickener, 1%-15% antifreezing agent, stabilizer 0.5%-10%, surplus are deionized water；State the mixture that component b is Penicillium notatum, nitrogen-fixing bacteria, hay bacillus.Macleaya cordata aqueous suspension agent biological pesticide provided by the invention does not use or uses less organic solvent, and generates without dust, to operator and user's safety.Macleaya cordata aqueous suspension agent biological pesticide can also uniformly mix dispersion with arbitrary proportion with water, be influenced by water quality small, and easy to use, environment not easy to pollute is a kind of water baseization formulations of pesticide of Environmental compatibility；And it is fast to play a role, strong drug action；With there is good market prospects.
Description
Technical field
The invention belongs to pesticide production technology field, especially a kind of macleaya cordata aqueous suspension agent biological pesticide and its preparation side
Method.
Background technique
Macleaya cordata belongs to Papaveraceae Macleaya plant, is a kind of biological " three medicines " product of fully natural green.In macleaya cordata
Alkaloid contained such as sanguinarine, Chelerythrine, bocconine etc. has the effect of good desinsection, antibacterial；It can be used as
Prevent and treat water fruits and vegetables, cereal crops, cotton pest and disease damage etc. biological pesticide raw material.And macleaya cordata botanical pesticide has
It is degradable to imitate ingredient, is one of optimal kind in existing biological pesticide, it is efficient, nontoxic, pollution-free, and have improvement soil
Effect.
Currently, the agent of macleaya cordata biological pesticide is mainly water dispersion granule, wettable powder, missible oil, missible oil, wettable
That there are drug effects is low for pulvis, environmental hazard is big；And dust is easy in the production and use process for water dispersion granule, wettable powder
It flies upward, organic solvent is volatile in the production and use process for missible oil, can greatly endanger caused by operator.Based on this,
The scholar of only a few studies new dosage form, as the one kind for the file that number of patent application is CN201610089104.3 contains
The composition pesticide and preparation method of sanguinarine (Macleaya cordata alkaloids), the dosage form of composition are aqua, soluble liquid, cream
Oil, water dispersion granule, wettable powder or suspending agent；When the dosage form is suspending agent, according to percent by weight, component
Specifically include that sanguinarine 0.1~30%, the second pesticide 0.1~80%, dispersing agent 5~20%, antifreezing agent 1~10%, thickener
0.5~5%, defoaming agent 0.3~5%, ultraviolet radiation protectant 0.1~10%, synergist 0.5~10%, deionized water 12.6~
92.4%；It is first to mix other auxiliary agents when preparation, is uniformly mixed through high speed shear, effective component sanguinarine and the second agriculture is added
Medicine, abrading-ball 2~3 hours in ball crusher make grain in 5mm hereinafter, can be made into the aqueous suspension formulations of the present composition.But
The patent does not consider that suspending agent enters the effect of inside plant tissues；Farm chemical ingredients in suspending agent are with molecule
Existing for form, the rate for entering inside plant tissues is slow, needs its drug effect of long period of time competence exertion, and
Farm chemical ingredients during this period of time also can external environment effect occur it is rotten.
Summary of the invention
In order to solve the above technical problems existing in the prior art, it is raw that the present invention provides a kind of macleaya cordata aqueous suspension agents
Object pesticide and preparation method thereof is realized particular by following technical scheme:
A kind of macleaya cordata aqueous suspension agent biological pesticide, by weight percentage, comprising: 99.7%-99.8% component a,
0.20-0.30% component b.
Preferably, the component a, by weight percentage, comprising: 1%-5% Macleaya cordata alkaloids, 5%-20% dispersion
Agent, 0.5%-5% thickener, 1%-15% antifreezing agent, stabilizer 0.5%-10%, surplus are deionized water.
Preferably, the dispersing agent is fatty acid methyl ester sulfonate, alkyl glycosides, fatty alcohol polyoxyethylene ether, alcohol ether carboxylic
One or more of hydrochlorate, potassium monoalkyl phosphate, polyethylene glycol, α olefin sulfonate and lauryl sodium sulfate.
Preferably, the stabilizer is one or more of bentonite, diatomite, precipitated calcium carbonate.
Preferably, the thickener is polyvinyl alcohol, xanthan gum, hydroxypropyl methyl cellulose, WJZ-04, di-2-ethylhexylphosphine oxide
One or more of naphthalene sulfonate, calcium lignosulfonate, gum arabic, polyvinyl alcohol.
Preferably, the antifreezing agent is one or more of ethylene glycol, glycerine, sodium chloride.
Preferably, the component b is the mixture of Penicillium notatum, nitrogen-fixing bacteria, hay bacillus.
The present invention also provides a kind of preparation methods of macleaya cordata aqueous suspension agent biological pesticide, including following procedure:
(4) preparation of component a: together with part deionization after Macleaya cordata alkaloids, dispersing agent, stabilizer are weighed according to the ratio
Water is added in sand mill, sanding media is added after pre-dispersed 5-15min, 0.5-1.5h is sanded；Then sequentially add thickener,
Antifreezing agent is modulated to obtain the homogeneous aqueous suspension agent of pale red to get component a；
(5) preparation of component b: a small amount of component a and MRS culture medium is taken to be mixed and made into liquid culture by the mass ratio ratio of 2-3:1
Base；Penicillium notatum, nitrogen-fixing bacteria, hay bacillus is taken to be inoculated in 3 liquid culture mediums respectively by the inoculum concentration of 3-4wt% in nothing respectively again
Collarium is cultivated in border；After strain covers with fluid nutrient medium, strain liquid is extracted from 3 liquid culture mediums respectively and presses 8-
The inoculum concentration of 10wt% is inoculated in the same fluid nutrient medium, and culture to strain covers with mixing liquid culture in gnotobasis
Base stirs evenly the mixing liquid culture medium for covering with strain to get component b.
(6) component a, component b precentagewise example are dispensed, cryo-conservation is to get macleaya cordata aqueous suspension agent biological pesticide.
Preferably, the application method of the macleaya cordata aqueous suspension agent biological pesticide are as follows: take component b that normal-temperature water dilution 2 is added
Times, after standing 10min or so, with being uniformly mixed for component a；Then 500 times or so are diluted to be sprayed on plant.
The beneficial effects of the present invention are:
The component b that the present invention uses is the mixture of Penicillium notatum, nitrogen-fixing bacteria, hay bacillus, and Penicillium notatum is macleaya cordata plant
Endogenetic fungus, nitrogen-fixing bacteria, hay bacillus are also endophyte common in plant；3 kinds of endophytes are added to macleaya cordata water
In deflocculant biological, this 3 kinds of fungies are readily permeable to arrive inside plant tissues, therefore can carry in the Pesticide Infiltration to tissue of part
Portion, can fast onset effect；And this 3 kinds of fungies can enhance the drug effect of pesticide, will not generate harm to people, animal and make
With.
Macleaya cordata aqueous suspension agent biological pesticide provided by the invention does not use or uses less organic solvent, and generates without dust,
To operator and user's safety.Macleaya cordata aqueous suspension agent biological pesticide can also uniformly mix dispersion with arbitrary proportion with water, by
The influence of water quality is small, easy to use, environment not easy to pollute, is a kind of water baseization formulations of pesticide of Environmental compatibility；And it plays
Effect is fast, strong drug action；With there is good market prospects.
Detailed description of the invention
Fig. 1 is the preparation flow figure of component a in macleaya cordata aqueous suspension agent biological pesticide of the present invention.
Specific embodiment
It is limited below with reference to specific embodiment technical solution of the present invention is further, but claimed
Range is not only limited to made description.
Example 1 group divides a to be 1% macleaya cordata aqueous suspension agent
99.7% component a of farm chemical ingredients, 0.30% component b.
Component a ingredient: Macleaya cordata alkaloids 10g, fatty alcohol polyoxyethylene ether 50g, bentonite 5g, gum arabic 5g,
Ethylene glycol 15g, surplus are water.
Preparation method:
(1) preparation of component a: connect after Macleaya cordata alkaloids 10g, fatty alcohol polyoxyethylene ether 50g, bentonite 5g are weighed
Be added in sand mill with deionized water 200ml, pre-dispersed 5min is added sanding media and 0.5h is sanded, by gum arabic 5g,
Ethylene glycol 15g and remaining water are added, and are modulated to obtain the homogeneous 1% macleaya cordata aqueous suspension agent of pale red to get component a；
(2) preparation of component b: a small amount of component a and MRS culture medium is taken to be mixed and made into liquid culture medium by the mass ratio ratio of 2:1；
Penicillium notatum, nitrogen-fixing bacteria, hay bacillus is taken to be inoculated in 3 liquid culture mediums respectively by the inoculum concentration of 3wt% in asepsis ring respectively again
It is cultivated in border；After strain covers with fluid nutrient medium, strain liquid connecing by 8wt% is extracted from 3 liquid culture mediums respectively
Kind amount is inoculated in the same fluid nutrient medium, and culture covers with mixing liquid culture medium to strain in gnotobasis, will be covered with
The mixing liquid culture medium of strain stirs evenly to get component b；
(3) by component a, component b by 99.7%, 0.30% component b percent mass ratio dispense, cryo-conservation to get
Macleaya cordata aqueous suspension agent biological pesticide.
2 component a of embodiment is 2% macleaya cordata aqueous suspension agent
Farm chemical ingredients: 99.7% component a, 0.30% component b.
Component a ingredient: Macleaya cordata alkaloids 20g, lauryl sodium sulfate 60g, diatomite 8g, polyvinyl alcohol 10g, the third three
Alcohol 10g, surplus are water.
Preparation method:
(1) it the preparation of component a: will be added in sand mill after weighing together with deionized water 300ml, pre-dispersed 10min adds
Enter to be sanded Jie's Macleaya cordata alkaloids 20g, lauryl sodium sulfate 60g, diatomite 8g matter and 1.0h is sanded, by polyvinyl alcohol 10g, third
Triol 10g and remaining water are added, and are modulated to obtain the homogeneous 2% macleaya cordata aqueous suspension agent aqueous suspension agent of pale red to get component
a；
(3) preparation of component b: a small amount of component a and MRS culture medium is taken to be mixed and made into liquid culture medium by the mass ratio ratio of 2:1；
Penicillium notatum, nitrogen-fixing bacteria, hay bacillus is taken to be inoculated in 3 liquid culture mediums respectively by the inoculum concentration of 4wt% in asepsis ring respectively again
It is cultivated in border；After strain covers with fluid nutrient medium, strain liquid connecing by 8wt% is extracted from 3 liquid culture mediums respectively
Kind amount is inoculated in the same fluid nutrient medium, and culture covers with mixing liquid culture medium to strain in gnotobasis, will be covered with
The mixing liquid culture medium of strain stirs evenly to get component b；
(7) component a, component b are dispensed by 80.0%, 20% percent mass ratio, cryo-conservation is to get macleaya cordata water
Deflocculant biological.
3 component a of embodiment is 3% macleaya cordata aqueous suspension agent
Farm chemical ingredients: 99.8% component a, 0.20% component b.
Component a ingredient: the double naphthalene sulfonate 5g of Macleaya cordata alkaloids 30g, alkyl glycosides 80g, precipitated calcium carbonate 10g, methyl,
Glycerine 15g, surplus are water.
Preparation method:
(1) preparation of component a: by Macleaya cordata alkaloids 30g, alkyl glycosides 80g, precipitated calcium carbonate 10g weigh after together with
Deionized water 300ml is added in sand mill, and pre-dispersed 10min is added sanding media and 0.5h is sanded, by di-2-ethylhexylphosphine oxide naphthalene sulfonic acids
Salt 5g, glycerine 15g and remaining water are added, and are modulated to obtain the homogeneous 3% macleaya cordata aqueous suspension agent of pale red to get component a；
(4) preparation of component b: a small amount of component a and MRS culture medium is taken to be mixed and made into liquid culture medium by the mass ratio ratio of 3:1；
Penicillium notatum, nitrogen-fixing bacteria, hay bacillus is taken to be inoculated in 3 liquid culture mediums respectively by the inoculum concentration of 3.2wt% sterile respectively again
It is cultivated in environment；After strain covers with fluid nutrient medium, strain liquid is extracted from 3 liquid culture mediums respectively by 10wt%'s
Inoculum concentration is inoculated in the same fluid nutrient medium, and culture covers with mixing liquid culture medium to strain in gnotobasis, will be grown
The mixing liquid culture medium of full strain stirs evenly to get component b；
(8) component a, component b are dispensed by 99.8% component a, 0.20% component b percent mass ratio, cryo-conservation, i.e.,
Obtain macleaya cordata aqueous suspension agent biological pesticide.
4 component a of embodiment is 5% macleaya cordata aqueous suspension agent
Farm chemical ingredients: 99.75% component a, 0.25% component b.
Component a ingredient: Macleaya cordata alkaloids 50g, lauryl sodium sulfate 100g, bentonite 15g, calcium lignosulfonate
15g, ethylene glycol 20g, surplus are water.
Preparation method:
(1) preparation of component a: connect after Macleaya cordata alkaloids 50g, lauryl sodium sulfate 100g, bentonite 15g are weighed
It is added in sand mill with deionized water 400ml, pre-dispersed 5min is added sanding media and 1.5h is sanded, by calcium lignosulfonate
15g, ethylene glycol 20g and remaining water are added, and are modulated to obtain the homogeneous 5% macleaya cordata aqueous suspension agent of pale red to get component a；
(5) preparation of component b: a small amount of component a and MRS culture medium is taken to be mixed and made into liquid culture medium by the mass ratio ratio of 3:1；
Penicillium notatum, nitrogen-fixing bacteria, hay bacillus is taken to be inoculated in 3 liquid culture mediums respectively by the inoculum concentration of 4wt% in asepsis ring respectively again
It is cultivated in border；After strain covers with fluid nutrient medium, strain liquid connecing by 10wt% is extracted from 3 liquid culture mediums respectively
Kind amount is inoculated in the same fluid nutrient medium, and culture covers with mixing liquid culture medium to strain in gnotobasis, will be covered with
The mixing liquid culture medium of strain stirs evenly to get component b；
(9) component a, component b are dispensed by 99.75% component a, 0.25% component b percent mass ratio, cryo-conservation,
Up to macleaya cordata aqueous suspension agent biological pesticide.
Comparative example 1
Comparative example 1 is not the difference from embodiment 1 is that have component b in macleaya cordata aqueous suspension agent biological pesticide.
Experimental example 1
Macleaya cordata aqueous suspension agent biological pesticide mixed with rice bran research:
Experimental method: the macleaya cordata aqueous suspension agent biological pesticide for taking 1-4 of the embodiment of the present invention and comparative example 1 to prepare is as real
Group is tested, using space management as control group, 20 plants of capsicums of every group of processing；Embodiment 1-4 group is by macleaya cordata aqueous suspension agent biology agriculture
Component b in medicine is added normal-temperature water and dilutes 2 times, after standing 10min or so, with being uniformly mixed for component a；Then 500 times are diluted
It is sprayed on capsicum.1 group of comparative example is that macleaya cordata aqueous suspension agent biological pesticide is directly diluted 500 times to be sprayed on capsicum
?.Control group is without any processing.One mu of capsicum of every group of processing is administered 1 time, and every group of spraying time is identical.Observe its control it is peppery
The state of an illness of green pepper, the results are shown in Table 1.
Diseased plant rate × 100% before preventive effect=(diseased plant rate before 1 diseased plant rate-medicine after medicine)/medication.
Table 1
As can be known from the above table, the preventive effect of 10d capsicum wilt is considerably higher in 1 group of comparative example after embodiment 1-4 group medicine；Implement
The preventive effect of 20d capsicum wilt is also above comparative example 1 after example 1-4 group medicine.Show component b used in the present invention in medication early period
Macleaya cordata alkaloids can be promoted to play sterilization functions, have the function of enhancing drug effect.
Experimental example 2
Macleaya cordata aqueous suspension agent biological pesticide prevents and treats the research of long bean aphid:
Experimental method: the macleaya cordata aqueous suspension agent biological pesticide for taking 1-4 of the embodiment of the present invention and comparative example 1 to prepare is as real
Group is tested, using space management as control group, 20 plants of capsicums of every group of processing；Embodiment 1-4 group is by macleaya cordata aqueous suspension agent biology agriculture
Component b in medicine is added normal-temperature water and dilutes 2 times, after standing 10min or so, with being uniformly mixed for component a；Then 500 times are diluted
It is sprayed on long bean.1 group of comparative example is that macleaya cordata aqueous suspension agent biological pesticide is directly diluted 500 times to be sprayed on long bean
?.Control group is without any processing.10 plants of long beans of every group of processing are administered 1 time, and every group of formulation rate is identical.5 sides of every plant of investigation
Position, each orientation use five point sampling, and the work aphid number of 20 centimeters of random searching tip top records and calculates out respectively at spray
5d, 10d are investigated after preceding and spray, and count aphid number living, calculate control efficiency.Investigation result such as table 2.
Long bean aphid insect population decrease aphid radix before rate (%)=(aphid survival number after aphid radix-medicine before medicine)/medicine ×
100%；
Preventive effect (%)=(the treatment region aphid rate of decreasing-blank district aphid decrease rate)/(100- blank district aphid
Worm decreases rate) × 100%.
Table 2
As can be known from the above table, the preventive effect of 5d is compared with comparative example 1 after embodiment 1-4 group medicine, the not no difference of conspicuousness；Implement
The preventive effect of 10d is higher than comparative example 1 after example 1-4 group medicine.Show that component b used in the present invention can not promote agriculture in medication early period
Medicine, which plays, kills pest effect, but has the function of centainly enhancing drug effect in the medication later period.
It is important to point out that, above embodiments and test example are only limitted to do further technical solution of the present invention herein
Elaboration and understanding, should not be understood as it is further to technical solution of the present invention limited, what those skilled in the art made
The innovation and creation of non-protruding essential characteristics and marked improvement still fall within protection category of the invention.
Claims (9)
1. a kind of macleaya cordata aqueous suspension agent biological pesticide, by weight percentage, comprising: 99.7%-99.8% component a, 0.20-
0.30% component b.
2. macleaya cordata aqueous suspension agent biological pesticide as described in claim 1, which is characterized in that the component a, by weight percentage
Than meter, comprising: 1%-5% Macleaya cordata alkaloids, 5%-20% dispersing agent, 0.5%-5% thickener, 1%-15% antifreezing agent,
Stabilizer 0.5%-10%, surplus are deionized water.
3. macleaya cordata aqueous suspension agent biological pesticide as claimed in claim 2, which is characterized in that the dispersing agent is fatty acid methyl
Sulfonated ester, alkyl glycosides, fatty alcohol polyoxyethylene ether, alcohol ether carboxylate, potassium monoalkyl phosphate, polyethylene glycol, α-alkene
One or more of base sulfonate and lauryl sodium sulfate.
4. macleaya cordata aqueous suspension agent biological pesticide as claimed in claim 2, which is characterized in that the stabilizer be bentonite,
One or more of diatomite, precipitated calcium carbonate.
5. macleaya cordata aqueous suspension agent biological pesticide as claimed in claim 2, which is characterized in that the thickener is polyethylene
Alcohol, xanthan gum, hydroxypropyl methyl cellulose, WJZ-04, di-2-ethylhexylphosphine oxide naphthalene sulfonate, calcium lignosulfonate, gum arabic,
One or more of polyvinyl alcohol.
6. macleaya cordata aqueous suspension agent biological pesticide as claimed in claim 2, which is characterized in that the antifreezing agent be ethylene glycol,
One or more of glycerine, sodium chloride.
7. macleaya cordata aqueous suspension agent biological pesticide as described in claim 1, which is characterized in that, the component b be Penicillium notatum,
The mixture of nitrogen-fixing bacteria, hay bacillus.
8. a kind of preparation method of the macleaya cordata aqueous suspension agent biological pesticide as described in claim 1-7, is being characterized in that, packet
Include following procedure:
(1) preparation of component a: add after Macleaya cordata alkaloids, dispersing agent, stabilizer are weighed according to the ratio together with portions of de-ionized water
Enter into sand mill, sanding media is added after pre-dispersed 5-15min, 0.5-1.5h is sanded；Then thickener, antifreeze is sequentially added
Agent is modulated to obtain the homogeneous aqueous suspension agent of pale red to get component a；
(2) preparation of component b: a small amount of component a and MRS culture medium is taken to be mixed and made into liquid culture medium by the mass ratio ratio of 2-3:1；Again
Penicillium notatum, nitrogen-fixing bacteria, hay bacillus is taken to be inoculated in 3 liquid culture mediums respectively by the inoculum concentration of 3-4wt% in asepsis ring respectively
It is cultivated in border；After strain covers with fluid nutrient medium, strain liquid is extracted from 3 liquid culture mediums respectively by 8-10wt%'s
Inoculum concentration is inoculated in the same fluid nutrient medium, and culture covers with mixing liquid culture medium to strain in gnotobasis, will be grown
The mixing liquid culture medium of full strain stirs evenly to get component b.
(3) component a, component b precentagewise example are dispensed, component b cryo-conservation is to get macleaya cordata aqueous suspension agent biological pesticide.
9. the preparation method of the macleaya cordata aqueous suspension agent biological pesticide as claimed in claim 8, is being characterized in that, institute
State the application method of macleaya cordata aqueous suspension agent biological pesticide are as follows: take component b that normal-temperature water is added and dilute 2 times, stand 10min or so
Afterwards, it is uniformly mixed with component a；Then 500 times or so are diluted to be sprayed on plant.
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| CN101352180A (en) * | 2008-09-12 | 2009-01-28 | 中国农业大学 | Macleaya cordata alkaloids for inhibiting plant pathogenic bacteria and preparation method thereof |
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