CN105494380A - Pesticide composition containing sanguinarine (macleaya alkaloids) and preparation method thereof - Google Patents
Pesticide composition containing sanguinarine (macleaya alkaloids) and preparation method thereof Download PDFInfo
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- CN105494380A CN105494380A CN201610089104.3A CN201610089104A CN105494380A CN 105494380 A CN105494380 A CN 105494380A CN 201610089104 A CN201610089104 A CN 201610089104A CN 105494380 A CN105494380 A CN 105494380A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
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Abstract
The invention relates to the technical field of pesticide, in particular to a pesticide composition containing sanguinarine (macleaya alkaloids). The pesticide composition is prepared from, by weight, 0.1-30% of first pesticide, 0.1-80% of second pesticide, 5-80% of auxiliaries and 14.9-94.8% of a solvent or filler. The first pesticide is sanguinarine. The second pesticide comprises one of amino-oligosaccharin, cnidium lactone, spinosad, santonin, azadirachtin, wilforgine, neochamaejasmin, hyoscyamine and matrine. The auxiliaries comprise one or more of surfactant, a synergistic agent, an emulsifier, an anti-freezing agent, a dispersing agent, a wetting agent, a thickening agent, an ultraviolet protecting agent, a defoaming agent and an adhering agent. The pesticide belongs to plant source compound pesticide, the compound synergistic effect is remarkable, the pesticide composition can be used for preventing and treating various plant diseases, and pollution of chemical pesticide to the environment is reduced.
Description
Technical field
The present invention relates to technical field of pesticide, contain composition pesticide and the preparation method of sanguinarine (Macleaya cordata alkaloids) in particular to one.
Background technology
Over the past thousands of years, people have employed multiple method to protect the crop of oneself from the infringement of sick, worm and other pest, wherein, use agricultural chemicals to be one of effective method the most.Early stage agricultural chemicals is mostly from plant.After the forties in 20th century, chemical synthetic pesticide gains great popularity because of the feature such as efficient, quick-acting, insecticidal spectrum is wide, and this makes the research of botanical pesticide and use once to be absorbed in low ebb.
In recent years along with national economy is fast-developing, people more and more focus on the sustainable development of agricultural production and the coordinated development of human and environment.In traditional fruits and vegetables, grain and medicinal plant are produced, pest control mainly relies on chemical pesticide, but chemical medications a large amount of for a long time causes the appearance creating the problems such as pesticide resistance, environmental pollution, harm humans health, causes country to have to limit the use of many chemical pesticides; After entry into the World Trade Organization, market competition is fiercer, the impact that the export of farm produce meets with repeatly " green barrier ", the outlet of serious restriction agricultural products in China.
How to reduce the chemicals residual quantity in fruits and vegetables, grain, medicinal plant, become the focus that governments at all levels and scholar and peasant pay close attention to.Therefore, research and development novel biopesticide seems particularly important, and oneself becomes the important trend ensureing human health and agricultural sustainable development to develop biopesticide.Botanical pesticide causes the concern of people again because of features such as it are efficient, low toxicity, low-residual, selectivity are high, pest not easily develops immunity to drugs, again the focus of current pesticide research is become, but now on the market can plant-derived agriculture kind also very rare, be difficult to meet needed for agricultural production.
In view of this, special proposition the present invention.
Summary of the invention
The first object of the present invention is to provide a kind of composition pesticide containing sanguinarine, and belong to plant source compound agricultural chemicals, composite mutual synergistic effect is obvious, can solve the problem that existing chemical pesticide contamination hazard is serious.
The second object of the present invention is the preparation method providing a kind of composition pesticide containing sanguinarine, this agricultural chemicals be can be made into multiple formulation, can carry out many-sided application according to agricultural production.
The third object of the present invention is the application providing a kind of composition pesticide containing sanguinarine, and this composition pesticide can be used for control tomato virus disease, watermelon blight, Wolfberry Aphid, matrimony vine thrips, Paratrioza sinica, matrimony vine two spotted spider mite, jujube tree jujube cecidomyiia, tomato trialeurodes vaporariorum, cabbage pieris, cucumber downy mildew.
In order to realize above-mentioned purpose of the present invention, spy by the following technical solutions:
Containing a composition pesticide for sanguinarine, according to percent by weight, its component comprises:
First agricultural chemicals 0.1 ~ 30%, second agricultural chemicals 0.1 ~ 80%, auxiliary agent 5 ~ 80%, solvent or filler 14.9 ~ 94.8%;
Described first agricultural chemicals is sanguinarine; Described second agricultural chemicals comprises the one in amino-oligosaccharide, Osthole, multiple killing teichomycin, fennel artemisin, nimbin, wilforgine, chamaejasmine, hyoscyamine, matrine;
Described auxiliary agent comprise in surfactant, synergist, emulsifier, antifreezing agent, dispersant, wetting agent, thickener, ultraviolet radiation protectant, defoamer and binding agent one or more;
Preferably, the formulation of described composition is aqua, soluble liquid, missible oil, water dispersion granule, wetting powder or suspending agent.
Sanguinarine (Sanguinarine), [C of molecular formula
20h
14nO
4]+, relative molecular mass is 332.The application's sanguinarine used is one of principle active component in the extract macleaya cordata biology total alkali of bloodroot macleaya cordata (Macleayacordata (Willd) R.Br).
One in sanguinarine and amino-oligosaccharide, Osthole, multiple killing teichomycin, fennel artemisin, nimbin, wilforgine, chamaejasmine, hyoscyamine, matrine is preferably carried out composite by the application, and have significant synergies, desinsection/bactericidal effect is good.
Concrete, described solvent can be organic solvent and/or deionized water, and organic solvent can be the mixture of one or more in ethyl acetate, cyclohexanone, methyl oleate, ethanol, methyl alcohol, acetone, dimethyl formamide;
Described filler can be white carbon, potter's clay, one or more in kaolin.
Ultraviolet radiation protectant is that the application is particularly preferred, and other auxiliary agents are selected from this area frequent species, concrete:
Described surfactant is one or more mixture in alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers and similar substance, castor oil and ethylene oxide adduct, aralkyl phenol polyethenoxy ether formaldehyde condensation products;
Described synergist is the mixture of one or more in organosilicon, Butacide, Octacide 264, alkyl carbonate, azone;
Described emulsifier is the mixture of one or more in alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers and similar substance thereof, Well201, Well205, M30, this Pan's series, tween;
Described antifreezing agent is ethylene glycol or propane diols;
Described dispersant is di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate, and polycarboxylate code name is LG-3, GY-D1252, GY-D1256, SNWGF-01, and lignosulfonates code name is one or more in 201107,201108;
Described wetting agent is soapberry powder, silkworm excrement, neopelex, pull open in powder one or more;
Described thickener is xanthans, carboxymethyl cellulose, carboxyethyl cellulose, methylcellulose, Magnesiumaluminumsilicate, in polyvinyl alcohol one or more;
Described defoamer is silicone oil, silicone compound, C
10-20saturated fat acid compounds, C
8-10one or more of fatty alcohol;
Described binding agent is xanthans.
Preferably, the composition pesticide containing sanguinarine as above, when described formulation is aqua, according to percent by weight, its component comprises:
Sanguinarine 0.1 ~ 30%, second agricultural chemicals 0.1 ~ 80%, surfactant 5 ~ 20%, synergist 0.1 ~ 20%, antifreezing agent 0.1 ~ 10%, ultraviolet radiation protectant 0.1 ~ 10%, organic solvent 0.1 ~ 20%, deionized water 14.9 ~ 94.4%.
Preferably, the composition pesticide containing sanguinarine as above, when described formulation is soluble liquid, according to percent by weight, its component comprises:
Sanguinarine 0.1 ~ 30%, second agricultural chemicals 0.1 ~ 80%, surfactant 5 ~ 20%, ultraviolet radiation protectant 0.1 ~ 10%, synergist 0.1 ~ 20%, organic solvent 14.9 ~ 94.6%.
Preferably, the composition pesticide containing sanguinarine as above, when described formulation is missible oil, according to percent by weight, its component mainly comprises:
Sanguinarine 0.1 ~ 30%, second agricultural chemicals 0.1 ~ 80%, emulsifier 5 ~ 10%, ultraviolet radiation protectant 0.1 ~ 10%, synergist 0.1 ~ 20%, organic solvent 14.9 ~ 94.6%.
Preferably, the composition pesticide containing sanguinarine as above, when described formulation is water dispersion granule, according to percent by weight, its component mainly comprises:
Sanguinarine 0.1 ~ 30%, second agricultural chemicals 0.1 ~ 80%, dispersant 3 ~ 12%, wetting agent 2 ~ 8%, disintegrant 0.1 ~ 12%, binding agent 0.1 ~ 5%, ultraviolet radiation protectant 0.1 ~ 10%, filler 8 ~ 84.5%.
Preferably, the composition pesticide containing sanguinarine as above, when described formulation is wetting powder, according to percent by weight, its component mainly comprises:
Sanguinarine 0.1 ~ 30%, second agricultural chemicals 0.1 ~ 80%, dispersant 3 ~ 12%, wetting agent 3 ~ 15%, ultraviolet radiation protectant 0.1 ~ 10%, filler 8 ~ 83.7%.
Preferably, the composition pesticide containing sanguinarine as above, when described formulation is suspending agent, according to percent by weight, its component mainly comprises:
Sanguinarine 0.1 ~ 30%, second agricultural chemicals 0.1 ~ 80%, dispersant 5 ~ 20%, antifreezing agent 1 ~ 10%, thickener 0.5 ~ 5%, defoamer 0.3 ~ 5%, ultraviolet radiation protectant 0.1 ~ 10%, synergist 0.5 ~ 10%, deionized water 12.6 ~ 92.4%.
Composition pesticide containing sanguinarine as above:
Described ultraviolet radiation protectant comprise in carboxymethyl cellulose, ascorbic acid, urea, starch, dextrin, Congo red, xanthans, plain chocolate one or more.
The preparation method of the composition pesticide containing sanguinarine as above, comprising:
Sanguinarine, the second agricultural chemicals, auxiliary agent are mixed with solvent or filler, pulverize, and carry out preparation, obtains the composition pesticide containing sanguinarine.
The application of composition pesticide in control tomato virus disease, watermelon blight, Wolfberry Aphid, matrimony vine thrips, Paratrioza sinica, matrimony vine two spotted spider mite, jujube tree jujube cecidomyiia, tomato trialeurodes vaporariorum, cabbage pieris, cucumber downy mildew containing sanguinarine as above.
Compared with prior art, beneficial effect of the present invention is:
1), the composition pesticide containing sanguinarine provided by the invention belongs to plant source compound agricultural chemicals, and composite mutual synergistic effect is obvious, reduces the pesticide resistance of germ and insect, to crop and people and Environmental security, can significantly improve preventive effect.
2), the composition pesticide containing sanguinarine provided by the invention can high malicious chemical pesticide in Substitute For Partial, reduces chemical pesticide dosage, reduces the residual of environmental pollution and agricultural product.
3), the composition pesticide containing sanguinarine provided by the invention can effectively reduce chemical pesticide usage amount, reduces chemical pesticide spraying times and reduces dispenser cost, reducing chemical pesticide and remain and reduce the pollution of chemical pesticide to environment.
Embodiment
Below in conjunction with embodiment, embodiment of the present invention are described in detail, but it will be understood to those of skill in the art that the following example only for illustration of the present invention, and should not be considered as limiting the scope of the invention.Unreceipted actual conditions person in embodiment, the condition of conveniently conditioned disjunction manufacturer suggestion is carried out.Agents useful for same or the unreceipted production firm person of instrument, be and can buy by commercially available the conventional products obtained.
Experimental example 1 prevents and treats tomato virus disease Toxicity Determination
For examination pathogen: tomato virus disease
Test method: adopt live body pot-culture method, each process 10 times, classification investigation disease index after 7d, calculate EC50 co-toxicity coefficient, result of the test is as shown in table 1.In result of the test: co-toxicity coefficient (CTC) >=120 indicates synergistic effect; 80 < CTC < represent summation action; CTC≤80 indicate antagonism.
Table 1 sanguinarine, amino-oligosaccharide and mixture thereof are to the toxicity test of tomato virus disease
As can be seen from table 1 result, the result of the test of different proportion proportioning shows, wherein sanguinarine and amino-oligosaccharide aqua all show stronger synergistic effect by effective dilution proportion, wherein sanguinarine: when amino-oligosaccharide is 1:20 ~ 20:1, synergistic effect is obvious.Sanguinarine and amino-oligosaccharide mixture have reasonability and feasibility, are worth land for growing field crops further genralrlization application.
The composite effect of sanguinarine amino-oligosaccharide is further illustrated below by embodiment 1 ~ 5.
Embodiment 1:5% sanguinarine amino-oligosaccharide aqua
0.5g sanguinarine, 4.5g amino-oligosaccharide, 10g methyl alcohol, 6g castor oil, 15g Butacide, 5g ethylene glycol, 8g ascorbic acid, deionized water supplies 100g;
Embodiment 2:8% sanguinarine amino-oligosaccharide aqua
2g sanguinarine, 6g amino-oligosaccharide, 10g cyclohexanone, 8g triphen ethyl phenol polyoxyethylene ether phosphate, 10g Octacide 264,5g propane diols, 8% carboxymethyl cellulose, deionized water supply 100g;
Embodiment 3:10% sanguinarine amino-oligosaccharide aqua
3g sanguinarine, 7g amino-oligosaccharide, 10g methyl alcohol, 10g sodium alkyl benzene sulfonate, 5g organosilicon, 5g ethylene glycol, 10g urea, deionized water supply 100g;
Embodiment 4:15% sanguinarine amino-oligosaccharide aqua
7g sanguinarine, 8g amino-oligosaccharide, 10g ethyl acetate, 8g phenethyl phenol polyethenoxy ether polyethenoxy ether, 5g azone, 5g ethylene glycol, 10g ascorbic acid, deionized water supply 100g;
Embodiment 5:20% sanguinarine amino-oligosaccharide aqua
10g sanguinarine, 10g amino-oligosaccharide, 20g methyl alcohol, 10g sodium alkyl benzene sulfonate, 10g azone, 5g propane diols, 10g carboxymethyl cellulose, deionized water supply 100g;
The preparation method of embodiment 1 ~ 5 is: mixed by each raw material, is stirring transparent in a kettle., after quality inspection is qualified, is making finished product and be embodiment 1 ~ 5.
Experimental example 2 sanguinarine amino-oligosaccharide aqua control tomato virus disease
Shaanxi Xiannong Bio-Technology Co., Ltd. has carried out the drug effect test of field zone of sanguinarine amino-oligosaccharide aqua control tomato virus disease in Jingyang County, Shaanxi Province in 2015.
Test article: syringe (10ml) 5, knapsack hand sprayer two, drop (200), Enamel jar (1000ml) 1, pencil 3, minute book 1, slide measure 1, pulling needle is some, and one, camera.
For studying thing: tomato (protecting field)
Experimental scheme and method:
1% sanguinarine wetting powder, 2% amino-oligosaccharide aqua and blank be totally 8 process, often processes repetition 3 times, often repeats area 20m
2, randomized arrangement.Test sprays 3 times altogether, is on October 24th, 2015, October 31, November 7 respectively; It is all consistent that the cultivation condition testing all communities comprises land type, cultivation management, tomato variety and upgrowth situation etc.At the tomato result initial stage at a time when test, carry out constant foliar spray with reagent agent dilution 500,1000,1500 times of liquid, every community is determined strain and is investigated 30 strains, records occurring degree on every strain blade, and calculates control efficiency.Calculate control efficiency.Investigation result is as table 1.
The killed grade scale in leaf portion is:
0 grade: asymptomatic;
1 grade: bright arteries and veins, light floral leaf;
3 grades: lobus cardiacus and middle leaf sheet floral leaf;
5 grades: lobus cardiacus and middle leaf sheet floral leaf, minority leaf malformation, shrinkage or plant are slightly downgraded;
7 grades: heavy floral leaf, most leaf malformation, shrinkage or plant are downgraded;
9 grades: heavy floral leaf, blade obviously deformity, show as line leaf, plant is seriously downgraded, even dead;
Investigate 1 time before dispenser, investigation diseased plant rate and disease index, then after the 3rd dispenser, 5d, 10d investigate diseased plant rate and control efficiency, investigate 3 times altogether;
Sick leaf rate (%)=sick number of sheets/investigate the total number of sheets × 100%;
Disease index=∑ (the sick number of sheets × relative level numerical value at different levels)/(investigating the total number of sheets × 9) × 100%;
Control efficiency (%)=(1-(before blank district medicine after disease index × treatment region medicine disease index)/(after blank district medicine before disease index × treatment region medicine disease index)) × 100%.
Table 2 sanguinarine amino-oligosaccharide aqua control tomato virus disease field control effectiveness test
As seen from Table 2, present composition embodiment 1 ~ 5 composition pesticide 30g/ mu, 60g/ mu, 90g/ mu are to control tomato virus disease, at 60-90 gram/acre, single dose kind is obviously better than for tomato virus disease control efficiency, and to tomato growth safety, field does not find bad reaction.
The composite effect of sanguinarine amino-oligosaccharide is further illustrated below by embodiment 6 ~ 10.
Embodiment 6:10% sanguinarine amino-oligosaccharide aqua
6g sanguinarine, 4g amino-oligosaccharide, 10g methyl alcohol, 6g castor oil, 15g Butacide, 5g ethylene glycol, 8g ascorbic acid, deionized water supplies 100g;
Embodiment 7:15% sanguinarine amino-oligosaccharide aqua
6g sanguinarine, 9g amino-oligosaccharide, 10g cyclohexanone, 8g triphen ethyl phenol polyoxyethylene ether phosphate, 10g Octacide 264,5g propane diols, 8% carboxymethyl cellulose, deionized water supply 100g;
Embodiment 8:20% sanguinarine amino-oligosaccharide aqua
10g sanguinarine, 10g amino-oligosaccharide, 10g methyl alcohol, 10g sodium alkyl benzene sulfonate, 5g organosilicon, 5g ethylene glycol, 10g urea, deionized water supply 100g;
Embodiment 9:25% sanguinarine amino-oligosaccharide aqua
5g sanguinarine, 20g amino-oligosaccharide, 10g ethyl acetate, 8g phenethyl phenol polyethenoxy ether polyethenoxy ether, 5g azone, 5g ethylene glycol, 10g ascorbic acid, deionized water supply 100g;
Embodiment 10:30% sanguinarine amino-oligosaccharide aqua
10g sanguinarine, 20g amino-oligosaccharide, 20g methyl alcohol, 10g sodium alkyl benzene sulfonate, 10g azone, 5g propane diols, 10g carboxymethyl cellulose, deionized water supply 100g;
The preparation method of embodiment 6 ~ 10 is: mixed by each raw material, is stirring transparent in a kettle., after quality inspection is qualified, is making finished product and be embodiment 1 ~ 10.
Experimental example 3 sanguinarine amino-oligosaccharide control watermelon blight
1% sanguinarine wetting powder, 2% amino-oligosaccharide aqua and blank be totally 8 process, and often process repetition 3 times, often process 20 strains, invention formulation consumption every mu is watered 50 kilograms and carries out even spraying, altogether dispenser 3 times.After one month, pull up watermelon seedling, vertical profile vascular bundle, observe incidence, investigate the state of an illness of each process according to the grade scale of watermelon blight, according to following formulae discovery disease index and relative control effect, investigation result is as table 3.
Disease index=Σ [sick progression × this grade of diseased plant number]/(investigation sum × the highest sick progression) × 100%;
Relative control effect (%)=(contrast disease index-process disease index)/contrast disease index × 100%.
The grade scale of watermelon blight:
0 grade: in stem, vascular bundle is normal, without variable color;
1 grade: vascular bundle less than 20% variable color in stem;
2 grades: vascular bundle 20%-40% variable color in stem;
3 grades: vascular bundle 40%-60% variable color in stem;
4 grades: vascular bundle 60%-80% variable color in stem;
5 grades: vascular bundle more than 80% variable color in stem.
Poisoning investigation method
Whether after dispenser, continuous 7d estimates medicament has poisoning to crop.
Table 3 sanguinarine amino-oligosaccharide aqua control watermelon blight field control effectiveness test
Find out from result of the test table 3, present composition embodiment 6 ~ 10 composition pesticide 30g/ mu is to control watermelon blight, composition pesticide sanguinarine amino-oligosaccharide aqua control watermelon blight successful is better than single dose kind, preventive effect is apparently higher than contrast medicament, and to watermelon growing safety, field does not find bad reaction.
Experimental example 4 sanguinarine and fructus cnidii complex are to Wolfberry Aphid Toxicity Determination
Materials and methods
Examination worm: Wolfberry Aphid indoor are for many generations raised with matrimony vine seedling, laboratory picking aphid of the same size.
Reagent agent: A.20.0% sanguinarine mother liquor; B.10.0% Osthole mother liquor; There is provided by Shaanxi Xiannong Bio-Technology Co., Ltd..
Assay method: adopt the method that NY/T1154.7-2006 recommends, segment two-sided tape being cut into 3cm long is attached to slide one end, then the scraps of paper on viscose glue are torn off with tweezers, choose the aphid that Individual Size is basically identical, healthy, provoke with little writing brush, its back to be attached on viscose glue and its feeler and foot are not stuck, sticky 30 of every slide.First transfer 4h in moisturizing condition, check with bitubular anatomical lens, aphid of checkmating is chosen and discards, then mends sticky.Then the slide leaching being stained with aphid is into the liquidly shaken gently, sucks adhesive surface and liquid around through taking-up in 5 seconds blotting paper, often process 4 times and repeat, often repeat 30, and establish not containing medicament corresponding organic solvent process in contrast.
Virulence is evaluated: sod cultivation is the same.Obtain the co-toxicity coefficient (CTC value) of various complex preparation by SunShi method, and pass judgment on the synergistic effect of mixture with CTC value.This patent is thought: be synergistic effect as CTC > 120, it is antagonism during CTC < 80,80 < CTC < 120 are summation action, then have remarkable synergistic effect during CTC > 200.
Results and analysis: the method adopting NY/T1154.7-2006 to recommend determines sanguinarine and Osthole to the indoor virulence of Wolfberry Aphid, the results are shown in Table 5.
Table 4 sanguinarine, Osthole and mixture thereof are to the virulence of Wolfberry Aphid
| Reagent agent | Regression equation | LC50(mg/L) | Correlation coefficient | Co-toxicity coefficient (CTC) |
| Sanguinarine | Y＝3.4231+1.7926x | 7.60 | 0.9947 | —— |
| Osthole | Y＝3.1082+1.4365x | 18.98 | 0.9278 | —— |
| Sanguinarine: Osthole (1:10) | Y＝3.5391+1.6522x | 15.75 | 0.9648 | 111.39 |
| Sanguinarine: Osthole (1:5) | Y＝3.4642+1.3548x | 13.82 | 0.9806 | 118.60 |
| Sanguinarine: Osthole (1:1) | Y＝3.4175+1.5772x | 10.15 | 0.9937 | 139.64 |
| Sanguinarine: Osthole (5:1) | Y＝3.4108+1.3781x | 9.54 | 0.9964 | 152.80 |
| Sanguinarine: Osthole (10:1) | Y＝3.42136+1.2888x | 9.48 | 0.9982 | 164.12 |
As can be seen from Table 4, sanguinarine, Osthole and mixture thereof are to Wolfberry Aphid, wherein sanguinarine and Osthole 1:1,5:1,10:1 mixture are respectively 139.64,152.80,164.12 to Wolfberry Aphid co-toxicity coefficient, all be greater than 120, there is obvious synergistic effect, all the other proportioning mixtures are respectively 111.39,118.60 to Wolfberry Aphid co-toxicity coefficient, are all less than 120.This shows that sanguinarine and Osthole 1:1,5:1,10:1 mixture have certain synergistic effect to Wolfberry Aphid.
Below further illustrate by embodiment 11 ~ 15.
Embodiment 11:5% sanguinarine Osthole soluble liquid
100g supplied by 0.5g sanguinarine, 4.5g Osthole, 10g benzene sodium alkyl sulfonate, 8g ascorbic acid, 15g Butacide, propane diols;
Embodiment 12:10% sanguinarine Osthole soluble liquid
2g sanguinarine, 8g Osthole, 10g castor oil, 8g carboxymethyl cellulose, 5g organosilicon, acetone supply 100g;
Embodiment 13:10% sanguinarine Osthole soluble liquid
5g sanguinarine, 5g Osthole, 10g styrylphenol polyoxyethylene ether, 10g ascorbic acid, 10g azone, acetone supply 100g;
Embodiment 14:15% sanguinarine Osthole soluble liquid
100g supplied by 12g sanguinarine, 3 Ostholes, 10g sodium alkyl benzene sulfonate, 10g CMC, 10g azone, ethylene glycol;
Embodiment 15:20% sanguinarine Osthole soluble liquid
100g supplied by 18g sanguinarine, 2g Osthole, 10g sodium alkyl benzene sulfonate, 10g CMC, 7g azone, propane diols;
The preparation method of embodiment 11 ~ 15 is: mixed by each raw material, is stirring transparent in a kettle., after quality inspection is qualified, is making finished product and be embodiment 11 ~ 15.
Experimental example 5 sanguinarine osthole for preventing and treating Wolfberry Aphid
Shaanxi Xiannong Bio-Technology Co., Ltd. has carried out the drug effect test of field zone of sanguinarine Osthole soluble liquid control Wolfberry Aphid in 2015 at Yinchuan of Ningxia Province.
Test article: syringe (10ml) 5, adopts Singapore profit agriculture HD-400 knapsack hand sprayer two, drop (200), Enamel jar (1000ml) 1, pencil 3, minute book 1, slide measure 1, pulling needle is some, and one, camera.
For studying thing: matrimony vine
Experimental scheme and method: 1% sanguinarine, 1% osthole water-emulsion agent and blank be totally 8 process, often process repetition 3 times, often repeats area 30m
2, randomized arrangement.It is all consistent that the cultivation condition testing all communities comprises land type, cultivation management, matrimony vine kind and upgrowth situation etc.During test, matrimony vine enters fruiting period, individual plante rate with aphis more than 90%, oil branches and leaves rate 21%, carry out constant foliar spray with reagent agent dilution 500,1000,1500 times of liquid, 5 orientation are investigated in every strain, and each orientation adopts five point samplings, the aphid number alive of random searching tip top 20 centimeters or 5 leaves, record and calculate and investigate respectively at 3d, 7d, 10d before spray medicine and after spray medicine, the aphid number and statistics is lived, calculates control efficiency.Investigation result is as table 6.
Wolfberry Aphid insect population decreases aphid radix × 100% before rate (%)=(before medicine after aphid radix-medicine aphid survival number)/medicine;
Preventive effect (%)=(the treatment region aphid rate of decreasing-blank district aphid decrease rate)/(100-blank district aphid decrease rate) × 100%;
Table 5 sanguinarine Osthole soluble liquid control Wolfberry Aphid field control effectiveness test
As seen from Table 5, present composition embodiment 11 ~ 15 composition pesticide dilutes 500 times of liquid to control Wolfberry Aphid, all more than 79% was reached to Wolfberry Aphid lice preventive effect in 3 days, all more than 83% was reached to Wolfberry Aphid preventive effect in 7 days, all more than 89% was reached to Wolfberry Aphid preventive effect in 10 days; Present composition embodiment 11 ~ 15 composition pesticide dilutes 1000 times of liquid to control Wolfberry Aphid, all more than 76% was reached to Wolfberry Aphid preventive effect in 3 days, all more than 80% was reached to Wolfberry Aphid preventive effect in 7 days, all more than 84% was reached to Wolfberry Aphid preventive effect in 10 days, apparently higher than contrast medicament, and to matrimony vine growth safety, field does not find bad reaction.
Experimental example 6 sanguinarine and multiple killing teichomycin is composite studies matrimony vine thrips indoor virulence
Materials and methods
Examination worm: matrimony vine thrips indoor are for many generations raised with matrimony vine seedling, laboratory picking aphid of the same size.
Reagent agent:
A.20.0% sanguinarine mother liquor; B.90.0% Osthole mother liquor; There is provided by Shaanxi Xiannong Bio-Technology Co., Ltd..
Experimental condition:
Temperature: 25 ± 1 DEG C, humidity: RH60-80%, illumination: L: D=(16: 8) h
Test method:
Electricity consumption analytical balance accurately takes reagent agent respectively, after former medicine is made into mother liquor with organic solvent, is diluted to the serial liquid of tool finite concentration gradient with water by experimental scheme dosage respectively.
Assay method:
Test method, with reference to " People's Republic of China (PRC) agricultural industry criteria NY/T1154.9-2008 ", adopts spray-on process, selects that raise continuously on matrimony vine seedling, that physiological status is consistent thrips as examination worm.The atomisation pressure of Potter spray tower is stabilized in 1.47 × 105Pa, and fog-spray nozzle first cleans 2 times with acetone, then cleans 2 times with distilled water.First choose the consistent examination worm of physiological status with writing brush to be no less than 10 and to put in people's culture dish, then culture dish is placed in Potter spray tower chassis and carries out metered dose, spouting liquid is 1mL, takes out examination worm, raise after liquid sedimentation 1min.Often process is no less than 4 repetitions, and establishes the process not containing medicament (containing all solvents and emulsifier) not make blank.
After process, 48h checks examination worm death condition, records total borer population and dead borer population respectively.
Virulence is evaluated:
Sod cultivation is the same.Obtain the co-toxicity coefficient (CTC value) of various complex preparation by SunShi method, and pass judgment on the synergistic effect of mixture with CTC value.This patent is thought: be synergistic effect as CTC > 120, it is antagonism during CTC < 80,80 < CTC < 120 are summation action, then have remarkable synergistic effect during CTC > 200.
Results and analysis
The method adopting NY/T1154.7-2006 to recommend determines sanguinarine and multiple killing teichomycin to the indoor virulence of matrimony vine thrips, the results are shown in Table 6.
Table 6 sanguinarine, multiple killing teichomycin and mixture thereof are to the virulence of matrimony vine thrips
As can be seen from Table 6, sanguinarine, multiple killing teichomycin and mixture thereof are to matrimony vine thrips, and wherein sanguinarine and multiple killing teichomycin mixture are all greater than 120 to matrimony vine thrips co-toxicity coefficient, have obvious synergistic effect.This shows that sanguinarine and multiple killing teichomycin mixture have certain synergistic effect to matrimony vine thrips.
Below further illustrate by embodiment 16 ~ 20:
Embodiment 16:6% sanguinarine multiple killing teichomycin suspending agent
1g sanguinarine, 5g multiple killing teichomycin, 10g sodium methylene bis-naphthalene sulfonate, 5g ethylene glycol, 2g xanthans, 3g silicone oil, 5g CMC, 5g Butacide, deionized water supply 100g;
Embodiment 17:15% sanguinarine multiple killing teichomycin suspending agent
1.6g sanguinarine, 13.4g multiple killing teichomycin, 8g sodium methylene bis-naphthalene sulfonate, 5g ethylene glycol, 3g xanthans, 3gC
10-20saturated fat acid compounds, 5g ascorbic acid, 5g Octacide 264, deionized water supply 100g;
Embodiment 18:26% sanguinarine multiple killing teichomycin suspending agent
2g sanguinarine, 24g multiple killing teichomycin, 8g sodium methylene bis-naphthalene sulfonate, 5g ethylene glycol, 5g xanthans, 3gC
10-20saturated fat acid compounds, 5g ascorbic acid, 5g organosilicon, water, deionized water supplies 100g;
Embodiment 19:6% sanguinarine multiple killing teichomycin suspending agent
5g sanguinarine, 1g multiple killing teichomycin, 10g sodium methylene bis-naphthalene sulfonate, 5g ethylene glycol, 2g xanthans, 3g silicone oil, 5g CMC, 5g Butacide, deionized water supply 100g;
Embodiment 20:15% sanguinarine multiple killing teichomycin suspending agent
13.4g sanguinarine, 1.6g multiple killing teichomycin, 8g sodium methylene bis-naphthalene sulfonate, 5g ethylene glycol, 3g xanthans, 3gC
10-20saturated fat acid compounds, 5g ascorbic acid, 5g Octacide 264, deionized water supply 100g;
Other auxiliary agents are first mixed, mix through high speed shear by the formula of embodiment 16 ~ 20, add active ingredient sanguinarine and multiple killing teichomycin, and in ball crusher, abrading-ball 2 ~ 3 hours, makes grain at below 5mm, namely can be made into the aqueous suspension formulations of the present composition.
Experimental example 7 sanguinarine multiple killing teichomycin control matrimony vine thrips
Xi Xian agriculture bio tech ltd has carried out the drug effect test of field zone of sanguinarine multiple killing teichomycin suspending agent control matrimony vine thrips in 2015 at Yinchuan of Ningxia Province.
Test article: syringe (10ml) 5, adopts Singapore profit agriculture HD-400 knapsack hand sprayer two, drop (200), Enamel jar (1000ml) 1, pencil 3, minute book 1, slide measure 1, pulling needle is some, and one, camera.
For studying thing: matrimony vine.
Experimental scheme and method: 1% sanguinarine wetting powder, 2.5% suspending agent and blank be totally 8 process, often process repetition 3 times, often repeats area 30m2, randomized arrangement.It is all consistent that the cultivation condition testing all communities comprises land type, cultivation management, matrimony vine kind and upgrowth situation etc.During test, matrimony vine enters the thrips hazard boom phase, adopts conventional spray-on process, and 5 orientation are investigated in every strain, and record and calculate and investigate respectively at 1d, 3d, 7d before spray medicine and after spray medicine, the thrips number and statistics is lived, calculates control efficiency.Investigation result is as table 7.
Preventive effect (%)=(the treatment region thrips rate of decreasing-blank district thrips decrease rate)/(100-blank district thrips decrease rate) × 100%
Table 7 sanguinarine multiple killing teichomycin suspending agent is to matrimony vine thrips field control effectiveness test result
As seen from Table 7, present composition embodiment 16 ~ 20 composition pesticide 30 grams/acre is to control matrimony vine thrips, and composition pesticide control matrimony vine thrips preventive effect of the present invention is all obviously better than single dose kind, and control efficiency is all remarkable, can delay insect and develop immunity to drugs; And to matrimony vine growth safety, field does not find bad reaction, is worth applying from now on.
Experimental example 8 sanguinarine and fennel artemisin are to Paratrioza sinica Toxicity Determination
Materials and methods
Examination worm: Paratrioza sinica (Paratriozasinica) picks up from field, laboratory picking examination of the same size worm.
Reagent agent: A.20.0% sanguinarine mother liquor; B.20% the female powder of fennel artemisin; There is provided by the first agriculture biotechnology in Shaanxi is limited.
Test method: electricity consumption analytical balance accurately takes reagent agent respectively, after former medicine is made into mother liquor with organic solvent, is diluted to the serial liquid of tool finite concentration gradient with water by experimental scheme dosage respectively.
Assay method: adopt the method that NY/T1154.7-2006 recommends, segment two-sided tape being cut into 3cm long is attached to slide one end, then the scraps of paper on viscose glue are torn off with tweezers, choose the examination worm that Individual Size is basically identical, healthy, provoke with little writing brush, its back to be attached on viscose glue and its feeler and foot are not stuck, sticky 30 of every slide.First transfer 4h in moisturizing condition, check with bitubular anatomical lens, examination worm of checkmating is chosen and discards, then mends sticky.Then the slide leaching being stained with examination worm is into the liquidly shaken gently, sucks adhesive surface and liquid around through taking-up in 5 seconds blotting paper, often process 4 times and repeat, often repeat 30, and establish not containing medicament corresponding organic solvent process in contrast.
Results and analysis: the method adopting NY/T1154.70-2006 to recommend determines matrine and sanguinarine to the indoor virulence of Paratrioza sinica, the results are shown in Table 8.
Table 8 sanguinarine, fennel artemisin and mixture thereof are to the virulence of Paratrioza sinica
As can be seen from Table 8, sanguinarine, fennel artemisin and mixture thereof are to Paratrioza sinica, and wherein sanguinarine and fennel artemisin mixture are all greater than 120 to Paratrioza sinica co-toxicity coefficient, have obvious synergistic effect.This shows that sanguinarine and fennel artemisin mixture have certain synergistic effect to Paratrioza sinica.
Below further illustrate by embodiment 21 ~ 25
Embodiment 21:3% sanguinarine fennel artemisin missible oil
0.5g sanguinarine, 2.5g fennel artemisin, 8g sodium alkyl benzene sulfonate, 8g ascorbic acid, 15g Butacide, dimethylbenzene supply 100g;
Embodiment 22:8% sanguinarine fennel artemisin missible oil
2g sanguinarine, 6g fennel artemisin, 10g sodium alkyl benzene sulfonate, 10g ascorbic acid, 15g azone, acetone supply 100g;
Embodiment 23:10% sanguinarine fennel artemisin missible oil
5g sanguinarine, 5g fennel artemisin, 10g phenethyl phenol polyethenoxy ether polyethenoxy ether, 10g are Congo red, 15g Butacide, dimethylbenzene supply 100g;
Embodiment 24:12% sanguinarine fennel artemisin missible oil
9g sanguinarine, 3g fennel artemisin, 10g phenethyl phenol polyethenoxy ether polyethenoxy ether, 10g are Congo red, 15g Butacide, dimethylbenzene supply 100g;
Embodiment 25:18% sanguinarine fennel artemisin missible oil
100g supplied by 15g sanguinarine, 3g fennel artemisin, 15g sodium alkyl benzene sulfonate, 10g CMC, 15g azone, ethylene glycol;
The formula of embodiment 21 ~ 25, mixed by each component, stir in a kettle., after quality inspection is qualified, gained finished product is embodiment 21 ~ 25.
Experimental example 9 sanguinarine fennel artemisin control Paratrioza sinica
Shaanxi Xiannong Bio-Technology Co., Ltd. has carried out the drug effect test of field zone of sanguinarine fennel artemisin EC matrimony vine thrips in 2015 at Yinchuan of Ningxia Province.
Test article: syringe (10ml) 5, adopts Singapore profit agriculture HD-400 knapsack hand sprayer two, drop (200), Enamel jar (1000ml) 1, pencil 3, minute book 1, slide measure 1, pulling needle is some, and one, camera.
For studying thing: matrimony vine.
Experimental scheme and method: 1% sanguinarine wetting powder, 3% fennel artemisin missible oil and blank be totally 8 process, often process repetition 3 times, often repeats area 30m
2, randomized arrangement.It is all consistent that the cultivation condition testing all communities comprises land type, cultivation management, matrimony vine kind and upgrowth situation etc.During test, Paratrioza sinica enters the hazard boom phase, adopts conventional spray-on process, and 5 orientation are investigated in every strain, and record and calculate and investigate respectively at 1d, 3d, 7d before spray medicine and after spray medicine, the wood louse number and statistics is lived, calculates control efficiency.Investigation result is as table 9.
Preventive effect (%)=(the treatment region wood louse rate of decreasing-blank district wood louse decrease rate)/(100-blank district wood louse decrease rate) × 100%.
Table 9 sanguinarine fennel artemisin aqua control Paratrioza sinica field control effectiveness test
As seen from Table 9, present composition embodiment 21 ~ 25 composition pesticide dilution 500,1000,1500 times of liquid are to control Paratrioza sinica, composition pesticide control Paratrioza sinica preventive effect of the present invention is all obviously better than single dose kind, insect can be delayed develop immunity to drugs, apparently higher than contrast medicament, and to matrimony vine growth safety, field does not find bad reaction.Be worth applying from now on.
Embodiment 26:3% sanguinarine nimbin soluble liquid
100g supplied by 0.5g sanguinarine, 2.5g nimbin, 10g benzene sodium alkyl sulfonate, 8g ascorbic acid, 15g Butacide, propane diols;
Embodiment 27:5% sanguinarine nimbin soluble liquid
1g sanguinarine, 4g nimbin, 10g castor oil, 8g carboxymethyl cellulose, 5g organosilicon, acetone supply 100g;
Embodiment 28:10% sanguinarine nimbin soluble liquid
5g sanguinarine, 5g nimbin, 10g styrylphenol polyoxyethylene ether, 10g ascorbic acid, 10g azone, acetone supply 100g;
Embodiment 29:15% sanguinarine nimbin soluble liquid
100g supplied by 12g sanguinarine, 3g nimbin, 10g sodium alkyl benzene sulfonate, 10g CMC, 10g azone, ethylene glycol;
Embodiment 30:18% sanguinarine nimbin soluble liquid
100g supplied by 15g sanguinarine, 3g nimbin, 10g sodium alkyl benzene sulfonate, 10g CMC, 7g azone, propane diols;
According to the formula of embodiment 25 ~ 30, each material component is mixed, stir transparent in a kettle., after quality inspection is qualified, make finished product.
Experimental example 10 sanguinarine nimbin soluble liquid control matrimony vine two spotted spider mite
Shaanxi Xiannong Bio-Technology Co., Ltd. has carried out the drug effect test of field zone of sanguinarine nimbin soluble liquid control matrimony vine two spotted spider mite in 2015 at Yinchuan of Ningxia Province.
Test article: syringe (10ml) 5, adopts Singapore profit agriculture HD-400 knapsack hand sprayer two, drop (200), Enamel jar (1000ml) 1, pencil 3, minute book 1, slide measure 1, pulling needle is some, and one, camera.
For studying thing: matrimony vine.
Experimental scheme and method: 1% sanguinarine wetting powder, 0.7% nimbin emulsion and blank be totally 8 process, often process repetition 3 times, often repeats area 30m2, randomized arrangement.It is all consistent that the cultivation condition testing all communities comprises land type, cultivation management, matrimony vine kind and upgrowth situation etc.Constant foliar spray is carried out, investigation complete stool worm amount with reagent agent dilution 500,1000,1500 times of liquid.Investigation two spotted spider mite radix before medicine, 1,3,7 day investigation two spotted spider mite borer population alive after medicine.By worm amount before and after each chemicals treatment dispenser, calculate two spotted spider mite decline rate, compared with blank district, calculate the control efficiency of each chemicals treatment, and carry out significance test by " DMRT " method.Calculate two spotted spider mite decline rate and Rectifying efficacies investigation result as shown in table 2.
Computing formula is as follows:
Matrimony vine two spotted spider mite decreases two spotted spider mite radix × 100% before rate (%)=(before medicine after two spotted spider mite radix-medicine two spotted spider mite survival number)/medicine;
Preventive effect (%)=(the treatment region two spotted spider mite rate of decreasing-blank district two spotted spider mite decrease rate)/(100-blank district two spotted spider mite decrease rate) × 100%.
Table 10 sanguinarine nimbin soluble liquid control matrimony vine two spotted spider mite field control effectiveness test
As seen from Table 10, present composition embodiment 26 ~ 30 composition pesticide dilutes 500 times of liquid to control matrimony vine two spotted spider mite, all more than 80% was reached to matrimony vine two spotted spider mite preventive effect in 1 day, all more than 90% was reached to matrimony vine two spotted spider mite preventive effect in 3 days, all more than 92% was reached to matrimony vine two spotted spider mite preventive effect in 7 days; Present composition embodiment 31,32,33,34,35 botanical composition dilutes 1000 times of liquid to control matrimony vine two spotted spider mite, all more than 78% was reached to matrimony vine two spotted spider mite preventive effect in 1 day, all more than 81% was reached to matrimony vine two spotted spider mite preventive effect in 3 days, all more than 85% was reached to matrimony vine two spotted spider mite preventive effect in 7 days.The present composition is all remarkable to the control efficiency of matrimony vine two spotted spider mite, and apparently higher than contrast medicament, and to matrimony vine growth safety, field does not find bad reaction.
The composite effect of 31 ~ 35 explanation sanguinarine and thunder godvine by the following examples
Embodiment 31:5% sanguinarine wilforgine missible oil
0.5g sanguinarine, 4.5g wilforgine, 5g sodium alkyl benzene sulfonate, 8g ascorbic acid, 15g Butacide, dimethylbenzene supply 100g;
Embodiment 32:12% sanguinarine wilforgine missible oil
3g sanguinarine, 9g wilforgine, 10g sodium alkyl benzene sulfonate, 10g ascorbic acid, 15g azone, acetone supply 100g;
Embodiment 33:15% sanguinarine wilforgine missible oil
7.5g sanguinarine, 7.5g wilforgine, 10g phenethyl phenol polyethenoxy ether polyethenoxy ether, 10g are Congo red, 15g Butacide, dimethylbenzene supply 100g;
Embodiment 34:20% sanguinarine wilforgine missible oil
15g sanguinarine, 5g wilforgine, 10g phenethyl phenol polyethenoxy ether polyethenoxy ether, 10g are Congo red, 15g Butacide, dimethylbenzene supply 100g;
Embodiment 35:24% sanguinarine wilforgine missible oil
100g supplied by 20g sanguinarine, 4g wilforgine, 15g sodium alkyl benzene sulfonate, 10g CMC, 15g azone, ethylene glycol;
According to the formula of embodiment 31 ~ 35, each component is mixed, stirs in a kettle., after quality inspection is qualified, get product;
Experimental example 11 sanguinarine wilforgine EC jujube tree jujube cecidomyiia
Shaanxi Xiannong Bio-Technology Co., Ltd. has carried out the drug effect test of field zone of sanguinarine wilforgine EC jujube tree jujube cecidomyiia in Aksu of Xinjiang in 2015.
Test article: syringe (10ml) 5, adopts Singapore profit agriculture HD-400 knapsack hand sprayer two, drop (200), Enamel jar (1000ml) 1, pencil 3, minute book 1, slide measure 1, pulling needle is some, and one, camera.
For studying thing: jujube tree.
Experimental scheme and method: 1% sanguinarine wetting powder, 1% wilforgine missible oil and blank be totally 8 process, often process repetition 3 times, often repeats area 50m2, randomized arrangement.It is all consistent that the cultivation condition testing all communities comprises land type, cultivation management, jujube cultivars and upgrowth situation etc.Constant foliar spray is carried out, investigation complete stool worm amount with reagent agent dilution 500,1000,1500 times of liquid.Investigation jujube cecidomyiia radix before medicine, 1,3,7 day investigation jujube cecidomyiia borer population alive after medicine.By worm amount before and after each chemicals treatment dispenser, calculate jujube cecidomyiia decline rate, compared with blank district, calculate the control efficiency of each chemicals treatment, and carry out significance test by " DMRT " method.Calculate jujube cecidomyiia decline rate and Rectifying efficacies investigation result as shown in table 11.
Computing formula is as follows:
Jujube tree jujube cecidomyiia decreases jujube cecidomyiia radix × 100% before rate (%)=(before medicine after jujube cecidomyiia radix-medicine jujube cecidomyiia survival number)/medicine;
Preventive effect (%)=(the treatment region jujube cecidomyiia rate of decreasing-blank district jujube cecidomyiia decrease rate)/(100-blank district jujube cecidomyiia decrease rate) × 100%.
Table 11 sanguinarine wilforgine EC jujube tree jujube cecidomyiia field control effectiveness test
As seen from Table 11, present composition embodiment 31 ~ 35 composition pesticide dilution 500,1000,1500 times of liquid are to control jujube tree jujube cecidomyiia, and all higher than single preparation variety preventive effect, and to jujube growth safety, field does not find bad reaction.
The composite effect of 36 ~ 40 explanation sanguinarine and chamaejasmine by the following examples:
Embodiment 36:3% sanguinarine chamaejasmine water dispersion granule
100g supplied by 1.4g sanguinarine, 1.6g chamaejasmine, 10g sodium methylene bis-naphthalene sulfonate, 10g soapberry powder, 8g ammonium sulfate, 3g xanthans, 5g CMC, white carbon;
Embodiment 37:8% sanguinarine chamaejasmine water dispersion granule
3g sanguinarine, 5g chamaejasmine, 10g sodium lignin sulfonate, 10g neopelex, 8g ammonium sulfate, 3g xanthans, 5g CMC, potter's clay supply 100g;
Embodiment 38:10% sanguinarine chamaejasmine water dispersion granule
2g sanguinarine, 8g chamaejasmine, 10g sodium lignin sulfonate, 10g neopelex, 8g aluminium chloride, 3g xanthans, 5g ascorbic acid, potter's clay supply 100g;
Embodiment 39:15% sanguinarine chamaejasmine water dispersion granule
5g sanguinarine, 10g chamaejasmine, 15g di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate, 10g neopelex, 10g citric acid, 5g xanthans, 8g ascorbic acid, potter's clay supply 100g;
Embodiment 40:20% sanguinarine chamaejasmine water dispersion granule
10g sanguinarine, 10g chamaejasmine, 15g di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate, 10g neopelex, 10g sodium bicarbonate, 5g xanthans, 8g ascorbic acid, potter's clay supply 100g;
According to the formula of embodiment 36 ~ 40, each component is mixed, obtain about 800 object particle diameters through air-flow crushing, then add binding agent mixing, obtain granulation materials.Item is quantitatively sent in fluidized bed granulating dryer through granulating and after drying, obtaining the water dispersible granules that sanguinarine chamaejasmine is composite, after quality inspection is qualified, get product;
Experimental example 12 sanguinarine chamaejasmine water dispersion granule control cabbage pieris
Shaanxi Xiannong Bio-Technology Co., Ltd. has carried out the drug effect test of field zone of sanguinarine chamaejasmine water dispersion granule control cabbage pieris in Jingyang County, Shaanxi Province in 2015.
Test article: syringe (10ml) 5, adopts Singapore profit agriculture HD-400 knapsack hand sprayer two, drop (200), Enamel jar (1000ml) 1, pencil 3, minute book 1, slide measure 1, pulling needle is some, and one, camera.
For studying thing: wild cabbage.
Experimental scheme and method: 1% sanguinarine wetting powder, 1.6% stellerin emulsion and blank be totally 8 process, often process repetition 3 times, often repeats area 30m2, randomized arrangement.It is all consistent that the cultivation condition testing all communities comprises land type, cultivation management, wild cabbage and upgrowth situation etc.Constant foliar spray is carried out, investigation complete stool worm amount with reagent agent dilution 500,1000,1500 times of liquid.Investigation cabbage caterpillar radix before medicine, 1,3,7 day investigation cabbage caterpillar borer population alive after medicine.By worm amount before and after each chemicals treatment dispenser, calculate cabbage caterpillar decline rate, compared with blank district, calculate the control efficiency of each chemicals treatment, and carry out significance test by " DMRT " method.Calculate cabbage caterpillar decline rate, investigation result is as shown in table 12.
Computing formula is as follows:
Cabbage pieris decreases cabbage caterpillar radix × 100% before rate (%)=(before medicine after cabbage caterpillar radix-medicine cabbage caterpillar survival number)/medicine
Preventive effect (%)=(the treatment region cabbage caterpillar rate of decreasing-blank district cabbage caterpillar decrease rate)/(100-blank district cabbage caterpillar decrease rate) × 100%
Table 12 sanguinarine chamaejasmine water dispersion granule control cabbage pieris field control effectiveness test
As seen from Table 12, present composition embodiment 36 ~ 40 composition pesticide dilution 500,1000,1500 times of liquid are to preventing and treating cabbage pieris all higher than single preparation variety and blank, and to wild cabbage growth safety, field does not find bad reaction.
The composite effect of 41 ~ 45 explanation sanguinarine and hyoscyamine by the following examples:
Embodiment 41:5% sanguinarine hyoscyamine wetting powder
100g supplied by 4g sanguinarine, 1g hyoscyamine, 10g neopelex, 8g di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate, 15g kaolin, 8g urea, white carbon;
Embodiment 42:10% sanguinarine hyoscyamine wetting powder
7g sanguinarine, 3g hyoscyamine, 12g soapberry powder, 8g di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate, 15g white carbon, 8g CMC, kaolin supply 100g;
Embodiment 43:15% sanguinarine hyoscyamine wetting powder
10g sanguinarine, 5g hyoscyamine, 12g soapberry powder, 8g di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate, 15g white carbon, 8g ascorbic acid, kaolin supply 100g;
Embodiment 44:20% sanguinarine hyoscyamine wetting powder
15g sanguinarine, 5g hyoscyamine, 12g soapberry powder, 8g di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate, 15g white carbon, 8g ascorbic acid, kaolin supply 100g;
Embodiment 45:25% sanguinarine hyoscyamine wetting powder
17g sanguinarine, 8g hyoscyamine, 12g soapberry powder, 8g di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate, 15g white carbon, 8g ascorbic acid, kaolin supply 100g;
According to the formula of embodiment 41 ~ 45, according to formula ratio each component mixed and stir half an hour, obtain about 800 object particle diameters through air-flow crushing, again mix half an hour, the wetting powder that obtained sanguinarine, hyoscyamine are composite, after quality inspection is qualified, gets product.
Experimental example 13 sanguinarine hyoscyamine wetting powder control cucumber trialeurodes vaporariorum
Shaanxi Xiannong Bio-Technology Co., Ltd. has carried out the drug effect test of field zone of sanguinarine hyoscyamine wetting powder control cucumber trialeurodes vaporariorum in Jingyang County, Shaanxi Province in 2015.
Test article: syringe (10ml) 5, adopts Singapore profit agriculture HD-400 knapsack hand sprayer two, drop (200), Enamel jar (1000ml) 1, pencil 3, minute book 1, slide measure 1, pulling needle is some, and one, camera.
For studying thing: cucumber.
Experimental scheme and method: 1% sanguinarine wetting powder, 1% hyoscyamine wetting powder and blank be totally 8 process, often process repetition 3 times, often repeats area 30m2, randomized arrangement.It is all consistent that the cultivation condition testing all communities comprises land type, cultivation management, cucumber variety and upgrowth situation etc.Constant foliar spray is carried out, investigation complete stool trialeurodes vaporariorum amount with reagent agent dilution 500,1000,1500 times of liquid.Investigation trialeurodes vaporariorum radix before medicine, 1,3,7 day investigation trialeurodes vaporariorum borer population alive after medicine.By worm amount before and after each chemicals treatment dispenser, calculate trialeurodes vaporariorum decline rate, compared with blank district, calculate the control efficiency of each chemicals treatment, and carry out significance test by " DMRT " method.Calculate trialeurodes vaporariorum decline rate, investigation result is as shown in table 13
Computing formula is as follows:
Cucumber trialeurodes vaporariorum decreases trialeurodes vaporariorum radix × 100% before rate (%)=(before medicine after trialeurodes vaporariorum radix-medicine trialeurodes vaporariorum survival number)/medicine
Preventive effect (%)=(the treatment region trialeurodes vaporariorum rate of decreasing-blank district trialeurodes vaporariorum decrease rate)/(100-blank district trialeurodes vaporariorum decrease rate) × 100%
Table 13 sanguinarine hyoscyamine wetting powder control cucumber trialeurodes vaporariorum field control effectiveness test
As seen from Table 13, present composition embodiment 41 ~ 45 composition pesticide dilution 500,1000,1500 times of liquid are to preventing and treating cucumber trialeurodes vaporariorum all higher than single preparation variety, and to cucumber growth safety, field does not find bad reaction.
The composite effect of 46 ~ 50 explanation sanguinarine and matrine by the following examples:
Embodiment 46:1% sanguinarine aqueous matrine solution
0.5g sanguinarine, 0.5g matrine, 10g methyl alcohol, 6g castor oil, 15g Butacide, 5g ethylene glycol, 5g ascorbic acid, deionized water supplies 100g;
Embodiment 47:3% sanguinarine aqueous matrine solution
1.2g sanguinarine, 1.8g matrine, 10g cyclohexanone, 8g triphen ethyl phenol polyoxyethylene ether phosphate, 10g Octacide 264,5g propane diols, 5% carboxymethyl cellulose, deionized water supply 100g;
Embodiment 48:5% sanguinarine aqueous matrine solution
1g sanguinarine, 4g matrine, 10g methyl alcohol, 10g sodium alkyl benzene sulfonate, 5g organosilicon, 5g ethylene glycol, 10g urea, deionized water supply 100g;
Embodiment 49:8% sanguinarine aqueous matrine solution
3g sanguinarine, 5g matrine, 10g ethyl acetate, 8g phenethyl phenol polyethenoxy ether polyethenoxy ether, 5g azone, 5g ethylene glycol, 5g ascorbic acid, deionized water supply 100g;
Embodiment 50:10% sanguinarine aqueous matrine solution
4g sanguinarine, 6g matrine, 20g methyl alcohol, 10g sodium alkyl benzene sulfonate, 10g azone, 5g propane diols, 5g carboxymethyl cellulose, deionized water supply 100g;
According to the formula of enforcement 46 ~ 50, each composition is mixed, stir transparent in a kettle., after quality inspection is qualified, make finished product.
Experimental example 14 illustrates the excellent results of above-mentioned medicament below by way of test
Table 14 sanguinarine, matrine and mixture thereof are to cucumber downy mildew virulence test
| Chemicals treatment | Virulence regression equation | Correlation coefficient (r) | Significance level | EC50(mg/L) |
| Sanguinarine | Y＝0.5895x+6.5323 | 0.984 | 0.000 | 35.9669 |
| Matrine | Y＝0.2602x+3.1967 | 0.864 | 0.007 | 31.5496 |
| Embodiment 46 | Y＝0.2368x+2.9323 | 0.882 | 0.005 | 34.6041 |
| Embodiment 47 | Y＝0.4341x+4.9815 | 0.913 | 0.101 | 32.2816 |
| Embodiment 48 | Y＝0.4940x+5.6365 | 0.967 | 0.003 | 30.5646 |
| Embodiment 49 | Y＝0.2104x+2.7088 | 0.982 | 0.002 | 27.5889 |
| Embodiment 50 | Y＝0.2133x+2.5204 | 0.991 | 0.000 | 25.8922 |
As can be seen from Table 14, sanguinarine, matrine and mixture thereof are to cucumber downy mildew, wherein sanguinarine and matrine mixture are to cucumber downy mildew control efficiency apparently higher than single dose kind, and this illustrates that sanguinarine and matrine mixture have obvious synergistic effect to cucumber downy mildew.
Experimental example 15 sanguinarine aqueous matrine solution control cucumber downy mildew
Shaanxi Xiannong Bio-Technology Co., Ltd. has carried out the drug effect test of field zone of sanguinarine aqueous matrine solution control cucumber downy mildew in Yangling District, Shaanxi Province in 2015.
Test article: syringe (10ml) 5, adopts Singapore profit agriculture HD-400 knapsack hand sprayer two, drop (200), Enamel jar (1000ml) 1, pencil 3, minute book 1, slide measure 1, pulling needle is some, and one, camera.
For studying thing: cucumber.
Experimental scheme and method: 1% sanguinarine wetting powder, 0.5% aqueous matrine solution and blank be totally 8 process, often process repetition 3 times, often repeats area 30m2, randomized arrangement.It is all consistent that the cultivation condition testing all communities comprises land type, cultivation management, cucumber variety and upgrowth situation etc.During test, cucumber enters young fruit period, reagent agent carries out constant foliar spray, the scab number of random searching 5 leaves, records and calculates and within 10 days, investigate after 10 days, the 3rd time spray medicine after 10 days, the 2nd time spray medicine respectively at before spray medicine and after the 1st spray medicine, and add up scab number, calculate control efficiency.Investigation result is as table 15.
Record occurring degree by staging, grade scale is as follows:
0 grade: anosis;
1 grade: sorus area accounts for less than 5% of whole leaf area;
3 grades: sorus area accounts for below the 6%-10% of whole leaf area;
5 grades: sorus area accounts for below the 11%-20% of whole leaf area;
7 grades: sorus area accounts for below the 21%-50% of whole leaf area;
9 grades: sorus area accounts for more than 50% of whole leaf area.
Drug effect computational methods
Sick leaf rate (%)=sick number of sheets/investigate the total number of sheets × 100%;
Disease index=∑ (the sick number of sheets × relative level numerical value at different levels)/(investigating the total number of sheets × 9) × 100%;
Control efficiency (%)=(1-(before blank district medicine after disease index × treatment region medicine disease index)/(after blank district medicine before disease index × treatment region medicine disease index)) × 100%.
Poisoning investigation method
Whether after dispenser, continuous 7d estimates medicament has poisoning to crop.
Result of the test and analysis
The effect of each process control cucumber downy mildew is in table 15.
Table 15 sanguinarine aqueous matrine solution control cucumber downy mildew field control effectiveness test
The Mixed Pharmacy of different proportion as can be seen from Table 15, tests by different consumptions, and after medicine, it is all better than contrasting medicament to the control of cucumber downy mildew, and bactericidal effect increases progressively with the increase of dosage.According to field range estimation, within the scope of test dose, plant growth is normal, and the poisoning phenomenon to cucumber does not all appear in each treatment agent, illustrates that it is safe to cucumber.
Although illustrate and describe the present invention with specific embodiment, however it will be appreciated that can to make when not deviating from the spirit and scope of the present invention many other change and amendment.Therefore, this means to comprise all such changes and modifications belonged in the scope of the invention in the following claims.
Claims (10)
1., containing a composition pesticide for sanguinarine, it is characterized in that, according to percent by weight, its component comprises:
First agricultural chemicals 0.1 ~ 30%, second agricultural chemicals 0.1 ~ 80%, auxiliary agent 5 ~ 80%, solvent or filler 14.9 ~ 94.8%;
Described first agricultural chemicals is sanguinarine; Described second agricultural chemicals comprises the one in amino-oligosaccharide, Osthole, multiple killing teichomycin, fennel artemisin, nimbin, wilforgine, chamaejasmine, hyoscyamine, matrine;
Described auxiliary agent comprise in surfactant, synergist, emulsifier, antifreezing agent, dispersant, wetting agent, thickener, ultraviolet radiation protectant, defoamer and binding agent one or more;
Preferably, the formulation of described composition is aqua, soluble liquid, missible oil, water dispersion granule, wetting powder or suspending agent.
2. the composition pesticide containing sanguinarine as claimed in claim 1, it is characterized in that, when described formulation is aqua, according to percent by weight, its component comprises:
Sanguinarine 0.1 ~ 30%, second agricultural chemicals 0.1 ~ 80%, surfactant 5 ~ 20%, synergist 0.1 ~ 20%, antifreezing agent 0.1 ~ 10%, ultraviolet radiation protectant 0.1 ~ 10%, organic solvent 0.1 ~ 20%, deionized water 14.9 ~ 94.4%.
3. the composition pesticide containing sanguinarine as claimed in claim 1, it is characterized in that, when described formulation is soluble liquid, according to percent by weight, its component comprises:
Sanguinarine 0.1 ~ 30%, second agricultural chemicals 0.1 ~ 80%, surfactant 5 ~ 20%, ultraviolet radiation protectant 0.1 ~ 10%, synergist 0.1 ~ 20%, organic solvent 14.9 ~ 94.6%.
4. the composition pesticide containing sanguinarine as claimed in claim 1, it is characterized in that, when described formulation is missible oil, according to percent by weight, its component mainly comprises:
Sanguinarine 0.1 ~ 30%, second agricultural chemicals 0.1 ~ 80%, emulsifier 5 ~ 10%, ultraviolet radiation protectant 0.1 ~ 10%, synergist 0.1 ~ 20%, organic solvent 14.9 ~ 94.6%.
5. the composition pesticide containing sanguinarine as claimed in claim 1, it is characterized in that, when described formulation is water dispersion granule, according to percent by weight, its component mainly comprises:
Sanguinarine 0.1 ~ 30%, second agricultural chemicals 0.1 ~ 80%, dispersant 3 ~ 12%, wetting agent 2 ~ 8%, disintegrant 0.1 ~ 12%, binding agent 0.1 ~ 5%, ultraviolet radiation protectant 0.1 ~ 10%, filler 8 ~ 84.5%.
6. the composition pesticide containing sanguinarine as claimed in claim 1, it is characterized in that, when described formulation is wetting powder, according to percent by weight, its component mainly comprises:
Sanguinarine 0.1 ~ 30%, second agricultural chemicals 0.1 ~ 80%, dispersant 3 ~ 12%, wetting agent 3 ~ 15%, ultraviolet radiation protectant 0.1 ~ 10%, filler 8 ~ 83.7%.
7. the composition pesticide containing sanguinarine as claimed in claim 1, it is characterized in that, when described formulation is suspending agent, according to percent by weight, its component mainly comprises:
Sanguinarine 0.1 ~ 30%, second agricultural chemicals 0.1 ~ 80%, dispersant 5 ~ 20%, antifreezing agent 1 ~ 10%, thickener 0.5 ~ 5%, defoamer 0.3 ~ 5%, ultraviolet radiation protectant 0.1 ~ 10%, synergist 0.5 ~ 10%, deionized water 12.6 ~ 92.4%.
8. the composition pesticide containing sanguinarine as described in any one of claim 1 ~ 7, is characterized in that:
Described ultraviolet radiation protectant comprise in carboxymethyl cellulose, ascorbic acid, urea, starch, dextrin, Congo red, xanthans, plain chocolate one or more.
9., containing a preparation method for the composition pesticide of sanguinarine, it is characterized in that, comprising:
Sanguinarine, the second agricultural chemicals, auxiliary agent are mixed with solvent or filler, pulverize, and carry out preparation, obtains the composition pesticide containing sanguinarine.
10. the application of composition pesticide in control tomato virus disease, watermelon blight, Wolfberry Aphid, matrimony vine thrips, Paratrioza sinica, matrimony vine two spotted spider mite, jujube tree jujube cecidomyiia, tomato trialeurodes vaporariorum, cabbage pieris, cucumber downy mildew containing sanguinarine described in any one of claim 1 ~ 8.
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| CN110961231A (en) * | 2019-12-23 | 2020-04-07 | 金晓飞 | Preparation method and application of macleaya cordata traditional Chinese medicinal material ultramicro wall-breaking powder |
| CN114885951A (en) * | 2022-04-13 | 2022-08-12 | 安徽丰乐农化有限责任公司 | Potato pesticide fertilizer and application thereof |
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