CN110249800A - The collection of balsam pear pollen and store method - Google Patents
The collection of balsam pear pollen and store method Download PDFInfo
- Publication number
- CN110249800A CN110249800A CN201910566627.6A CN201910566627A CN110249800A CN 110249800 A CN110249800 A CN 110249800A CN 201910566627 A CN201910566627 A CN 201910566627A CN 110249800 A CN110249800 A CN 110249800A
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- CN
- China
- Prior art keywords
- balsam pear
- pollen
- collection
- store method
- pear pollen
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01D—HARVESTING; MOWING
- A01D91/00—Methods for harvesting agricultural products
- A01D91/04—Products growing above the soil
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
Abstract
The invention discloses a kind of collection of balsam pear pollen and store methods, the vigor for the balsam pear pollen that this method can be maintained effectively, successfully solve in production process because of the problem that balsam pear pollination caused by natural environment is bad and the reasons such as flowering asynchronism is difficult, to cultivate New variety of balsam pear, seed production has important application value.
Description
Technical field
The invention belongs to plant pollination technique fields, and in particular to a kind of collection of balsam pear pollen and store method.
Background technique
Balsam pear is distributed widely in the tropical and subtropical region in Africa and Asia, is a kind of vegetables of dietotherapeutic, by vast
Consumer likes, economic value with higher.Currently, the mode of crossbreeding is mostly used to obtain the excellent new product of Comprehensive Traits
Kind, the collection and preservation of pollen are to realize the important link of balsam pear artificial hybridization breeding.However, currently without about balsam pear pollen
The mature technology collected and saved.During the production of hybrid seeds, mostly use bloom the previous day press from both sides flower method, be only applicable to pollen adopt i.e. with
It pollinates between the kind of flower synchronization, and pollen activity is often influenced by weather conditions.Since balsam pear is diclinism plant, and it is each
Female, florescence differ greatly between kind, often because of difficult problem of pollinating caused by flowering asynchronism.
Summary of the invention
The purpose of the present invention is to provide a kind of collection of balsam pear pollen and store methods.
The technical solution used in the present invention is:
A kind of collection of balsam pear pollen and store method, include the following steps:
(1) bud before acquisition is bloomed removes sepal and corolla, retains holder and stamen；
(2) stamen with holder collected step (1) is dry to anther dehiscence；
(3) impurity removing is removed in sieving, collects anther, seals cryo-conservation.
It is described bloom before bud refer to the bud of the previous day of blooming, the corolla of bud in yellow, sufficiently expand but do not open
It puts.
Preferably, step (2) is dry under the conditions of 20~30 DEG C.
Preferably, the sieving referred to 100-200 mesh.
Preferably, the sieving of step (3) anther is placed on 4 DEG C or -80 DEG C are sealed.
The beneficial effects of the present invention are: present inventor is on the basis of long-term production practices, it is different by comparing
It is high in short term to form a kind of practical balsam pear pollen easy to operate for the influence of picking time and holding conditions to balsam pear pollen germination rate
Storage technique is imitated, the vigor for the balsam pear pollen that can effectively maintain successfully solves in production process because of natural environment not
The problem that the reasons such as good and flowering asynchronism cause balsam pear pollination difficult has cultivation New variety of balsam pear, seed production important
Application value.
Detailed description of the invention
Fig. 1 is that the wild type balsam pear pollen that the method for the present invention is collected saves the 5th day in 4 DEG C, pollen germination rate 63%.
Fig. 2 is that the wild type balsam pear pollen that this method is collected saves 1 month in -80 DEG C, pollen germination rate 60%.
Fig. 3 is control group, collects the wild type balsam pear pollen that the same day opens and saves the 5th day in 4 DEG C, pollen germination rate is
23%.
Fig. 4 is that the big top balsam pear pollen that the method for the present invention is collected saves the 5th day in 4 DEG C, pollen germination rate 52%.
Fig. 5 is that the big top balsam pear pollen that this method is collected saves 1 month in -80 DEG C, pollen germination rate 55%.
Fig. 6 is control group, collects the big top balsam pear pollen that the same day opens and saves the 5th day in 4 DEG C, pollen germination rate is
18%.
Specific embodiment
Below with reference to embodiment, the present invention is further illustrated, and however, it is not limited to this.
Embodiment 1: the collection of wild type balsam pear pollen and store method
1, the acquisition of bud
Acquisition standard: picking the bud for the previous day of blooming, and corolla yellow is sufficiently expanded but do not opened, and anther does not crack.
2, the collection of anther
It with tweezers or manually peels off corolla and sepal, exposes stamen, retain holder.
3, the drying of anther
The anther (band holder) of collection is laid on an A4 paper, (temperature is 25 DEG C or so) is dry in a cool and dry place
12 hours (overnight), make pollen crack.
4, the preservation of pollen
The stainless steel sieve that the anther of cracking and the pollen to shed are poured into 200 mesh, is placed on above a clean A4 matter, holds
A4 matter paper roll funnel is poured pollen in 2ml centrifuge tube into, is sealed with sealed membrane, put as needed by the pollen for taking over sieve
Enter 4 DEG C or -80 DEG C of refrigerators save.
5. pollen germination rate detects: after pollen is scattered in pollen cultures base upper 2h, being sprouted in microscopically observation pollen
Heat condition, 5 × or 10 × object, 5 visuals field under the microscope, counts its germination rate to grow pollen tube as sprouting standard.
Pollen germination rate=sprouting pollen frequence/observation pollen frequence × 100%.
Testing result shows that under the conditions of 4 DEG C, pollen can be reserved for 5-7 days, and pollen germination rate is 63% (see Fig. 1)；-80
It under the conditions of DEG C, can be reserved for 1 month, pollen germination rate is 60% (see Fig. 2).
The formula of pollen cultures base: 10% sucrose, 10mg/L boric acid, 0.5% agar.
As control, collects the wild type balsam pear pollen that the same day opens and saved in 4 DEG C, the 5th day detection pollen germination rate is
23% (see Fig. 3).
Above-mentioned experiment is repeated several times, and pollen germination rate testing result is basicly stable, using the method for the present invention collect, save
Balsam pear pollen germination rate is above 50%.
Embodiment 2: the collection of big top balsam pear pollen and store method
1, the acquisition of bud
Acquisition standard: picking the bud for the previous day of blooming, and corolla yellow is sufficiently expanded but do not opened, and anther does not crack.
2, the collection of anther
It with tweezers or manually peels off corolla and sepal, exposes stamen, retain holder.
3, the drying of anther
The anther (band holder) of collection is laid on an A4 paper, dries 12 hours (overnight) in a cool and dry place,
Pollen is set to crack.
4, the preservation of pollen
The stainless steel sieve that the anther of cracking and the pollen to shed are poured into 200 mesh, is placed on above a clean A4 matter, holds
A4 matter paper roll funnel is poured pollen in 2ml centrifuge tube into, is sealed with sealed membrane, put as needed by the pollen for taking over sieve
Enter 4 DEG C or -80 DEG C of refrigerators save.
5, pollen germination rate detects
After pollen is scattered in pollen cultures base upper 2h, in microscopically observation pollen germination situation, testing result table
Bright, under the conditions of 4 DEG C, pollen be can be reserved for 5-7 days, and pollen germination rate is 52% (see Fig. 4)；Under the conditions of -80 DEG C, 1 can be reserved for
Month, pollen germination rate is 55% (see Fig. 5).
As control, collects the big top balsam pear pollen that the same day opens and saved in 4 DEG C, the 5th day detection pollen germination rate is
18% (see Fig. 6).
Above-mentioned experiment is repeated several times, and pollen germination rate testing result is basicly stable, using the method for the present invention collect, save
Balsam pear pollen germination rate is above 50%.
Claims (5)
1. collection and the store method of a kind of balsam pear pollen, include the following steps:
(1) bud before acquisition is bloomed removes sepal and corolla, retains holder and stamen；
(2) stamen with holder collected step (1) is dry to anther dehiscence；
(3) impurity removing is removed in sieving, collects anther, seals cryo-conservation.
2. collection and the store method of balsam pear pollen according to claim 1, which is characterized in that it is described bloom before bud
Refer to that the bud for the previous day of blooming, the corolla of bud are in yellow, sufficiently expand but do not open.
3. collection and the store method of balsam pear pollen according to claim 1, which is characterized in that step (2) be 20~
It is dry under the conditions of 30 DEG C.
4. collection and the store method of balsam pear pollen according to claim 1, which is characterized in that the sieving referred to
100-200 mesh.
5. collection and the store method of balsam pear pollen according to claim 1, which is characterized in that the sieving of step (3) anther
It is placed on 4 DEG C or -80 DEG C is sealed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910566627.6A CN110249800A (en) | 2019-06-27 | 2019-06-27 | The collection of balsam pear pollen and store method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910566627.6A CN110249800A (en) | 2019-06-27 | 2019-06-27 | The collection of balsam pear pollen and store method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110249800A true CN110249800A (en) | 2019-09-20 |
Family
ID=67922264
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910566627.6A Pending CN110249800A (en) | 2019-06-27 | 2019-06-27 | The collection of balsam pear pollen and store method |
Country Status (1)
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009040703A (en) * | 2007-08-07 | 2009-02-26 | National Agriculture & Food Research Organization | Method for preserving pollen |
| CN105028391A (en) * | 2015-07-02 | 2015-11-11 | 云南农业大学 | Eustoma grandiflorum pollen ultralow temperature preservation method |
| CN105165597A (en) * | 2015-08-07 | 2015-12-23 | 江苏丘陵地区镇江农业科学研究所 | Pollination method for kiwifruit |
| CN105567783A (en) * | 2016-03-10 | 2016-05-11 | 中国农业科学院特产研究所 | Preservation and in-vitro culture vitality detection method for pollen of Actinidia arguta |
| CN105614799A (en) * | 2016-03-22 | 2016-06-01 | 福建省农业科学院果树研究所 | Preparation method for plum pollen |
| CN107897174A (en) * | 2017-11-28 | 2018-04-13 | 芜湖县海琦生态农业有限公司 | A kind of activation method of the store method of Pulp Citrulli Pollen and corresponding pollen |
-
2019
- 2019-06-27 CN CN201910566627.6A patent/CN110249800A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009040703A (en) * | 2007-08-07 | 2009-02-26 | National Agriculture & Food Research Organization | Method for preserving pollen |
| CN105028391A (en) * | 2015-07-02 | 2015-11-11 | 云南农业大学 | Eustoma grandiflorum pollen ultralow temperature preservation method |
| CN105165597A (en) * | 2015-08-07 | 2015-12-23 | 江苏丘陵地区镇江农业科学研究所 | Pollination method for kiwifruit |
| CN105567783A (en) * | 2016-03-10 | 2016-05-11 | 中国农业科学院特产研究所 | Preservation and in-vitro culture vitality detection method for pollen of Actinidia arguta |
| CN105614799A (en) * | 2016-03-22 | 2016-06-01 | 福建省农业科学院果树研究所 | Preparation method for plum pollen |
| CN107897174A (en) * | 2017-11-28 | 2018-04-13 | 芜湖县海琦生态农业有限公司 | A kind of activation method of the store method of Pulp Citrulli Pollen and corresponding pollen |
Non-Patent Citations (1)
| Title |
|---|
| 温健新等: "保存大顶苦瓜花粉方法的应用研究初报", 《长江蔬菜》 * |
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Application publication date: 20190920 |
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