CN105614799A - Preparation method for plum pollen - Google Patents
Preparation method for plum pollen Download PDFInfo
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- CN105614799A CN105614799A CN201610162995.0A CN201610162995A CN105614799A CN 105614799 A CN105614799 A CN 105614799A CN 201610162995 A CN201610162995 A CN 201610162995A CN 105614799 A CN105614799 A CN 105614799A
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- Prior art keywords
- pollen
- bud
- anther
- flower
- drying
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Abstract
The invention belongs to the field of pollen collection and particularly relates to a preparation method for plum pollen. The preparation method comprises the following steps: 1) collecting flower buds: selecting a proper male parent according to a breeding target and collecting the flower buds in a large-bud stage together with flower stalks before 10:00 in the morning after a plum tree enters an initial flowering stage; 2) collecting anther: cutting off the upper half parts of the flower buds from the flower buds along the middle of each flower bud by using scissors, collecting the cut parts together, filtering out petals by using a 140-mesh sieve, and collecting the plum anther; 3) drying the anther: putting the collected anther into a culture dish and drying the anther in a constant-temperature culture box with the temperature of 30 DEG C; shaking the culture dish for multiple times during drying to promote the drying of the anther; and 4) storing pollen: sub-packaging the dried pollen in 1.5ml centrifugal pipes and storing the pipes in a refrigerator with the temperature of 4 DEG C for later use. The method is convenient to operate, simple, feasible and favorable for quickly obtaining a large amount of high-quality plum pollen.
Description
Technical field
The invention belongs to the assembling sphere of pollen, it is specifically related to the preparation method of a kind of Lee's pollen.
Background technology
Lee is Rosaceae Lee's platymiscium, has long cultivation history in China, and its taste of fruit is sweet, full of fragrance, very popular. Therefore, the seed selection of Lee's new variety is one of problem of domestic and international research worker Lee concern always. Cross-breeding is a kind of simplicity, easy breeding method, can, according to breeding objective on purpose Selection parent, be the important channel cultivating Lee's new variety. Obtaining a large amount of high-quality pollen is the important step carrying out Lee's cross-breeding.
Summary of the invention
It is an object of the invention to for the deficiencies in the prior art, it is provided that the preparation method of a kind of Lee's pollen. The present invention sieves after directly flower pesticide and petal upper part together being cut with scissors collection flower pesticide, it is not necessary to remove petal, convenient to operation, simple, is conducive to obtaining a large amount of high-quality Lee's pollen fast.
For achieving the above object, the present invention adopts following technical scheme:
A preparation method for Lee's pollen, comprises the following steps:
1) collection of bud: select suitable male parent according to breeding objective, enters after initial bloom stage until Japanese plum, before morning 10, adopts down together with the bud of anthocaulus by the big flower bud phase;
2) collection of flower pesticide: bud upper part cut from bud in the middle part of bud with scissors, collects the part sheared off after together, and screens out petal with 140 object sieves, collects Lee's flower pesticide;
3) drying of flower pesticide: the flower pesticide being collected is placed in culture dish, and-be put in the constant incubator of 30 DEG C to be dried 2 days; Rock culture dish between dry epoch for several times, promote that flower pesticide is dry;
4) preservation of pollen: the pollen of drying is dispensed in the centrifuge tube of 1.5ml, and for subsequent use in 4 DEG C of Refrigerator stores.
The useful effect of the present invention is:
Lee provided by the present invention spends powder, preparation method thereof, it is not necessary to remove petal, convenient to operation, simple, is conducive to obtaining a large amount of high-quality Lee's pollen fast.
Accompanying drawing explanation
Lee's bud of Fig. 1 collection;
The pollen that Fig. 2 collects.
Embodiment
The present invention's the following example illustrates the present invention further, but protection scope of the present invention is not limited to the following example.
Embodiment 1
A preparation method for Lee's pollen, concrete steps are:
1) collection of bud: after lotus Japanese plum enters initial bloom stage, before morning 10, adopts down together with the bud of anthocaulus by the big flower bud phase;
2) collection of flower pesticide: in the middle part of Fu-Rong plums flower bud, bud upper part is cut from bud with scissors, the part sheared off is collected after together, screens out petal with 140 object sieves, collect Fu-Rong plums flower pesticide
3) drying of flower pesticide: the flower pesticide being collected is placed in culture dish, and be put in the constant incubator of 30 DEG C and be dried 2 days; Rock culture dish between dry epoch for several times, promote that flower pesticide is dry;
4) preservation of pollen: the pollen of drying is dispensed in the centrifuge tube of 1.5ml, and for subsequent use in 4 DEG C of Refrigerator stores.
The foregoing is only the better embodiment of the present invention, all impartial changes done according to the present patent application patent scope, with modifying, all should belong to the covering scope of the present invention.
Claims (1)
1. the preparation method of Lee's pollen, it is characterised in that: comprise the following steps:
1) collection of bud: select suitable male parent according to breeding objective, enters after initial bloom stage until Japanese plum, before morning 10, adopts down together with the bud of anthocaulus by the big flower bud phase;
2) collection of flower pesticide: bud upper part cut from bud in the middle part of bud with scissors, collects the part sheared off after together, and screens out petal with 140 object sieves, collects Lee's flower pesticide;
3) drying of flower pesticide: the flower pesticide being collected is placed in culture dish, and be put in the constant incubator of 30 DEG C and be dried 2 days; Rock culture dish between dry epoch for several times, promote that flower pesticide is dry;
4) preservation of pollen: the pollen of drying is dispensed in the centrifuge tube of 1.5ml, and for subsequent use in 4 DEG C of Refrigerator stores.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610162995.0A CN105614799A (en) | 2016-03-22 | 2016-03-22 | Preparation method for plum pollen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610162995.0A CN105614799A (en) | 2016-03-22 | 2016-03-22 | Preparation method for plum pollen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105614799A true CN105614799A (en) | 2016-06-01 |
Family
ID=56030496
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610162995.0A Pending CN105614799A (en) | 2016-03-22 | 2016-03-22 | Preparation method for plum pollen |
Country Status (1)
| Country | Link |
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| CN (1) | CN105614799A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110249800A (en) * | 2019-06-27 | 2019-09-20 | 广东省农业科学院蔬菜研究所 | The collection of balsam pear pollen and store method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103222424A (en) * | 2013-05-16 | 2013-07-31 | 湖北省农业科学院果树茶叶研究所 | Method for quickly preparing pear flower powder |
| CN103719076A (en) * | 2013-12-25 | 2014-04-16 | 广东省农业科学院果树研究所 | Litchi pollen collecting and storing method |
| CN104322216A (en) * | 2013-11-19 | 2015-02-04 | 辽宁省农业科学院园艺分院 | Method for collecting and storing tulip pollen |
| CN105265304A (en) * | 2014-07-30 | 2016-01-27 | 河北省农林科学院昌黎果树研究所 | Chinese chestnut cross pollination method |
-
2016
- 2016-03-22 CN CN201610162995.0A patent/CN105614799A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103222424A (en) * | 2013-05-16 | 2013-07-31 | 湖北省农业科学院果树茶叶研究所 | Method for quickly preparing pear flower powder |
| CN104322216A (en) * | 2013-11-19 | 2015-02-04 | 辽宁省农业科学院园艺分院 | Method for collecting and storing tulip pollen |
| CN103719076A (en) * | 2013-12-25 | 2014-04-16 | 广东省农业科学院果树研究所 | Litchi pollen collecting and storing method |
| CN105265304A (en) * | 2014-07-30 | 2016-01-27 | 河北省农林科学院昌黎果树研究所 | Chinese chestnut cross pollination method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110249800A (en) * | 2019-06-27 | 2019-09-20 | 广东省农业科学院蔬菜研究所 | The collection of balsam pear pollen and store method |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160601 |
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| RJ01 | Rejection of invention patent application after publication |