CN110228852A - A method of administering aquiculture waste water - Google Patents
A method of administering aquiculture waste water Download PDFInfo
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- CN110228852A CN110228852A CN201910555999.9A CN201910555999A CN110228852A CN 110228852 A CN110228852 A CN 110228852A CN 201910555999 A CN201910555999 A CN 201910555999A CN 110228852 A CN110228852 A CN 110228852A
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- chlorella
- cfu
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
- C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/327—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae characterised by animals and plants
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
Abstract
The present invention provides a kind of methods for administering aquiculture waste water, and this method includes aquiculture waste water being introduced to cultivation to have in nacreous pond, and chlorella, bacillus subtilis and bacillus coagulans are added in breeding wastewater.This method can be obviously improved nitrogen phosphorus ligands efficiency in breeding wastewater, enhance the regulation effect of breeding wastewater.
Description
Technical field
This application involves field of waste water treatment, specifically, being related to a kind of method for administering aquiculture waste water.
Background technique
With the continuous improvement of people 's material life level, aquaculture industry has also obtained great development, but simultaneously
Since raiser covets the maximization of interests, as cultivation density is increasing, fish body excreta, residual bait etc. are deposited on
Pool bottom is more and more thicker, and siltation keeps pond available depth more and more shallow.Bed mud largely discharges ammonia nitrogen, nitrite, vulcanization
The harmful wastes such as object, sulfate cause breeding water body serious eutrophication, water environment degradation, directly destruction Cultivated water ecology
This part water is directly discharged to rivers and lakes again by balance, raiser, leads to the water quality deterioration of rivers and lakes, and considerably beyond
The self-bearing capacity of rivers and lakes makes rivers and lakes a series of serious environmental problems, aquatic ecosystem safety occur
It is seriously threatened, the environmental improvement of aquaculture system has been very urgent.
The administering method of existing aquiculture waste water is mostly to add to administer agent in breeding wastewater, and regulation effect is bad
And treatment cost is high, can not farthest strive for the support and cooperation at aquaculture family, so, it is badly in need of a kind of breeding wastewater
Processing method, both can be with the eutrophication of effective solution breeding water body the problems such as, the warp at aquaculture family can also be taken into account
Ji benefit.
Summary of the invention
For the bad problem of existing breeding wastewater regulation effect, the disclosure is designed to provide a kind of improvement aquatic products
The method of breeding wastewater, specifically, regulation effect in breeding wastewater can be obviously improved, promote nitrogen in breeding wastewater by providing one kind
The method of phosphorus ligands efficiency.
To achieve the goals above, present disclose provides it is a kind of administer aquiculture waste water method, this method it is main
Step are as follows: aquiculture waste water, which is introduced to cultivation, to be had in nacreous pond, and by chlorella, bacillus subtilis and
Bacillus coagulans are added in the breeding wastewater.
Preferably, the cultivation has in nacreous pond, and nacreous cultivation density is 800-1200 per acre;Phase
For aquiculture waste water described in every liter, the input amount of chlorella is (5-10) × 108CFU, the input amount of bacillus subtilis
For (60-120) × 108CFU, the input amount of bacillus coagulans are (50-100) × 108CFU。
It is furthermore preferred that nacreous cultivation density is 950-1100 per acre;It is useless relative to aquaculture described in every liter
Water, the input amount of chlorella are (6-8) × 108CFU, the input amount of bacillus subtilis are (80-110) × 108CFU, condensation
The input amount of bacillus is (70-90) × 108CFU。
Preferably, the chlorella is added in the form of the chlorella algae solution of logarithmic growth phase.
Preferably, the chlorella algae solution of the logarithmic growth phase passes through the method included the following steps and obtains:
S1, chlorella algae strain is pressed every liter 104-105The inoculum concentration of CFU is inoculated in liquid enriched medium, in 2000-
Under 3000lux intensity of illumination, at 25-30 DEG C after shaking flask culture 2-3 days, chlorella frond is harvested by centrifugation,
S2, using chlorella frond as the operation of chlorella algae strain return step S1, repeat 3-4 times, after being strengthened
Chlorella frond;
S3, the chlorella frond after reinforcing is pressed every liter 105-106The inoculum concentration of CFU is inoculated in liquid enriched medium,
At 3000-4000lux intensity of illumination, 25-30 DEG C after shaking flask culture 5-6 days, it is harvested by centrifugation to obtain the chlorella after light stimulus
Frond;
S4, chlorella frond is pressed every liter 106-107The inoculum concentration of CFU is inoculated in liquid enriched medium, is added to 30
In DEG C constant temperature incubation device, culture reaches 10 until cell density under aerobic condition8CFU·mL-1, obtain growth logarithmic phase
Chlorella algae solution.
Wherein, the liquid enriched medium includes following component: NaNO31900-2100mg/L, K2HPO4 0.04-
0.06mg/L, citric acid 5.5-6.5mg/L.
Wherein, the chlorella is the chlorella purchased from Xie Yuan Biotechnology Co., Ltd, strand city, the withered grass gemma
Bacillus is to create the bacillus subtilis that the article number of Biotechnology Co., Ltd is 68038-70-0, institute purchased from Xiamen Sheng Langsai
Stating bacillus coagulans is the condensation gemma bar for being far 68038-65-3 at the article number for creating Science and Technology Ltd. purchased from Wuhan
Bacterium.
Preferably, chlorella, bacillus subtilis and bacillus coagulans are being added to it in the breeding wastewater
Afterwards, the time of improvement is 30-120 days.
Through the above technical solutions, filtering food planktonic algae and organic debris using pearl freshwater mussel, the transparency of water body is improved;
Larger molecular organics conversion is decomposed into small molecule compound using bacillus subtilis and bacillus coagulans;Utilize bead
Algae absorbs small molecule compound, while discharging oxygen, promotes the dissolved oxygen in water body, produces one and is suitable for bacillus life
Long ecological environment.By the mutualism relationship between bacterium-algae-freshwater mussel, their own Ecology Action is improved, to aquatic products
Breeding wastewater carries out restoration of the ecosystem, is effectively improved eutrophication water body water quality.
Other feature and advantage of the disclosure will the following detailed description will be given in the detailed implementation section.
Specific embodiment
The specific embodiment of the disclosure is described in detail below.It should be understood that described herein specific
Embodiment is only used for describing and explaining the disclosure, is not limited to the disclosure.
Present disclose provides it is a kind of administer aquiculture waste water method, specifically: aquiculture waste water is introduced to feeding
Growing has in nacreous pond, and chlorella, bacillus subtilis and bacillus coagulans are added to the breeding wastewater
In.
Preferably, the cultivation has in nacreous pond, and nacreous cultivation density is 800-1200 per acre;Phase
For aquiculture waste water described in every liter, the input amount of chlorella is (5-10) × 108CFU, the input amount of bacillus subtilis
For (60-120) × 108CFU, the input amount of bacillus coagulans are (50-100) × 108CFU。
It is furthermore preferred that nacreous cultivation density is 950-1100 per acre;It is useless relative to aquaculture described in every liter
Water, the input amount of chlorella are (6-8) × 108CFU, the input amount of bacillus subtilis are (80-110) × 108CFU, condensation
The input amount of bacillus is (70-90) × 108CFU;It is still more preferably pearl freshwater mussel 1000, relative to described in every liter
Aquiculture waste water, chlorella 7 × 108CFU, bacillus subtilis 90 × 108CFU, bacillus coagulans 80 × 108CFU
Preferably, the chlorella is added in the form of the chlorella algae solution of logarithmic growth phase, chlorella density at this time
Height, activity are strong.
Preferably, the chlorella algae solution of the logarithmic growth phase passes through the method included the following steps and obtains:
S1, chlorella algae strain is pressed every liter 104-105The inoculum concentration of CFU is inoculated in liquid enriched medium, in 2000-
Under 3000lux intensity of illumination, at 25-30 DEG C after shaking flask culture 2-3 days, chlorella frond is harvested by centrifugation,
S2, using chlorella frond as the operation of chlorella algae strain return step S1, repeat 3-4 times, after being strengthened
Chlorella frond;
S3, the chlorella frond after reinforcing is pressed every liter 105-106The inoculum concentration of CFU is inoculated in liquid enriched medium,
At 3000-4000lux intensity of illumination, 25-30 DEG C after shaking flask culture 5-6 days, it is harvested by centrifugation to obtain the chlorella after light stimulus
Frond;
S4, chlorella frond is pressed every liter 106-107The inoculum concentration of CFU is inoculated in liquid enriched medium, is added to 30
In DEG C constant temperature incubation device, culture reaches 10 until cell density under aerobic condition8CFU·mL-1, obtain growth logarithmic phase
Chlorella algae solution.
Wherein, the liquid enriched medium includes following component: NaNO31900-2100mg/L, K2HPO4 0.04-
0.06mg/L, citric acid 5.5-6.5mg/L.
Wherein, the chlorella is the chlorella purchased from Xie Yuan Biotechnology Co., Ltd, strand city, the withered grass gemma
Bacillus is to create the bacillus subtilis that the article number of Biotechnology Co., Ltd is 68038-70-0, institute purchased from Xiamen Sheng Langsai
Stating bacillus coagulans is the condensation gemma bar for being far 68038-65-3 at the article number for creating Science and Technology Ltd. purchased from Wuhan
Bacterium.
Preferably, chlorella, bacillus subtilis and bacillus coagulans are being added to it in the breeding wastewater
Afterwards, the time of improvement is 30-120 days.
Hereinafter, the disclosure is further described by embodiment.
Chlorella algae solution in embodiment and comparative example is the chlorella algae strain bought being in of carrying out that hot housing obtains
The chlorella algae solution of logarithmic phase;Bacillus subtilis bacterium solution is withered after cultivating the bacillus subtilis strain bought
Careless bacillus bacterium solution;Bacillus coagulans bacterium solution is to condense gemma after cultivating the coagulating bacillus strain bought
Bacillus bacterium solution.
The Liquid Culture based formulas of bacillus subtilis are as follows: beef extract 2.0g, peptone 5.0g, yeast powder 3.0g,
MnSO40.005g, NaCl 2.0g, K2HPO43.0g, MgSO40.02g, pH 7.0 prepares mother liquor, high-temp steam sterilizing
Afterwards, cooling stand-by.The condition of culture of bacillus subtilis are as follows: the strain of bacillus subtilis is inoculated into mother liquor, 34-36 DEG C
Lower 100r/min shaking table culture 16 hours.
The formula of the fluid nutrient medium of bacillus coagulans are as follows: beef extract 2.0g, peptone 5.0g, yeast powder 3.0g,
MnSO40.005g, NaCl 2.0g, K2HPO43.0g, MgSO40.02g, pH 7.0 prepares mother liquor, high-temp steam sterilizing
Afterwards, cooling stand-by.The condition of culture of bacillus coagulans are as follows: the strain of bacillus coagulans is inoculated into mother liquor, 40-45 DEG C
Lower 180r/min shaking table culture 48 hours.
Embodiment 1
The method that the present embodiment illustrates the improvement aquiculture waste water of the disclosure, administers the reality of aquiculture waste water
Applying place is Hefei City, Anhui Province, and the area for choosing aquaculture pond is 10 mu, and the depth of water is 3 meters, and the time is in September, 2018.
Specific administering method are as follows: in aquaculture pond, introduce aquiculture waste water, put per acre in breeding wastewater
Enter pearl freshwater mussel 1000, relative to aquiculture waste water described in every liter, while putting into 7 × 108The chlorella algae solution of CFU, 90 ×
108The bacillus subtilis bacterium solution of CFU and 80 × 108The bacillus coagulans bacterium solution of CFU is administered 60 days.
Embodiment 2
The enforcement place that the present embodiment administers aquiculture waste water is Hefei City, Anhui Province.Other conditions and 1 phase of embodiment
Together.
Specific administering method are as follows: in aquaculture pond, introduce aquiculture waste water, put per acre in breeding wastewater
Enter pearl freshwater mussel 900, relative to aquiculture waste water described in every liter, while putting into 5.5 × 108The chlorella algae solution of CFU, 105
×108The bacillus subtilis bacterium solution of CFU and 95 × 108The bacillus coagulans bacterium solution of CFU is administered 60 days.
Embodiment 3
The enforcement place that the present embodiment administers aquiculture waste water is Hefei City, Anhui Province.Other conditions and 1 phase of embodiment
Together.
Specific administering method are as follows: in aquaculture pond, introduce aquiculture waste water, put per acre in breeding wastewater
Enter pearl freshwater mussel 1150, relative to aquiculture waste water described in every liter, while putting into 9 × 108The chlorella algae solution of CFU, 75 ×
108The bacillus subtilis bacterium solution of CFU and 62 × 108The bacillus coagulans bacterium solution of CFU is administered 60 days.
Comparative example 1
The enforcement place that this comparative example administers aquiculture waste water is Hefei City, Anhui Province.Other conditions and 1 phase of embodiment
Together.
Specific administering method are as follows: in aquaculture pond, introduce aquiculture waste water, while putting into 7 × 108CFU's
Chlorella algae solution is administered 60 days.
Comparative example 2
The enforcement place that this comparative example administers aquiculture waste water is Hefei City, Anhui Province.Other conditions and 1 phase of embodiment
Together.
Specific administering method are as follows: in aquaculture pond, aquiculture waste water is introduced, relative to aquatic products described in every liter
Breeding wastewater investment 90 × 108The bacillus subtilis bacterium solution of CFU and 80 × 108The bacillus coagulans bacterium solution of CFU administers 60
It.
Comparative example 3
The enforcement place that this comparative example administers aquiculture waste water is Hefei City, Anhui Province.Other conditions and 1 phase of embodiment
Together.
Specific administering method are as follows: in aquaculture pond, aquiculture waste water is introduced, relative to aquatic products described in every liter
Breeding wastewater investment 7 × 108The chlorella algae solution of CFU, 90 × 108The bacillus subtilis bacterium solution of CFU and 80 × 108CFU's
Bacillus coagulans bacterium solution is administered 60 days.
Test case 1
The cultivation detected in breeding wastewater and embodiment 1-3, comparative example 1-3 without improvement after 60 days administer is useless
The concentration of total nitrogen, ammonia nitrogen and total phosphorus in water, specific detection method are as follows: total nitrogen: alkaline persulfate clears up photometry;Ammonia nitrogen:
Berthelot spectrophotometry;Total phosphorus: ammonium molybdate spectrophotometric method.Embodiment 1-3 and comparative example 1- is calculated according to testing result
Nitrogen, phosphorus ligands effect ratio in 3, the results are shown in Table 1.
Wherein, nitrogen removal rate calculation method are as follows:
Nitrogen removal rate=(CTotal nitrogen concentration in breeding wastewater-CTo total nitrogen concentration in test case)/CTotal nitrogen concentration in breeding wastewater× 100%
Ammonia nitrogen removal frank calculation method are as follows:
Ammonia nitrogen removal frank=(CAmmonia nitrogen concentration in breeding wastewater-CTo ammonia nitrogen concentration in test case)/CAmmonia nitrogen concentration in breeding wastewater× 100%
Total tp removal rate calculation method are as follows:
Total tp removal rate=(CTotal phosphorus concentration in breeding wastewater-CTo total phosphorus concentration in test case)/CTotal phosphorus concentration in breeding wastewater× 100%
Table 1
It is analyzed by the data to table 1, nitrogen removal rate, ammonia nitrogen removal frank and the total tp removal rate of 3 embodiments
Obviously higher than 3 comparative examples.Therefore, compared to the list of chlorella and bacillus subtilis bacterium solution, bacillus coagulans bacterium solution
It solely uses or is used in mixed way, controlled jointly using pearl freshwater mussel, bacillus subtilis bacterium solution, bacillus coagulans bacterium solution and chlorella
The better effect of reason breeding wastewater is particularly put into pearl freshwater mussel 1000, relative to described in every liter per acre in breeding wastewater
Aquiculture waste water, while putting into 7 × 108The chlorella algae solution of CFU, 90 × 108The bacillus subtilis bacterium solution of CFU and 80
×108When the bacillus coagulans bacterium solution of CFU, administering in the time on an equal basis, regulation effect is best.It follows that the disclosure is logical
The mutualism relationship established between bacterium-algae-freshwater mussel is crossed, their own Ecology Action is improved, aquatic products is supported to realize
The restoration of the ecosystem of waste water is grown, and then eutrophication water body water quality can be effectively improved.
Above embodiments are only that the preferred embodiment of the disclosure is described, and are not defined to the scope of the present disclosure,
It, should all to all variations and modifications that the technical solution of the disclosure is made under the premise of not departing from the design spirit of the disclosure
It falls into the protection scope of the disclosure.
Claims (8)
1. a kind of method for administering aquiculture waste water, which is characterized in that this method comprises: aquiculture waste water is introduced to feeding
Growing has in nacreous pond, and chlorella, bacillus subtilis and bacillus coagulans are added to the breeding wastewater
In.
2. according to the method described in claim 1, wherein, the cultivation has in nacreous pond, nacreous cultivation density
Only for 800-1200 per acre;Relative to aquiculture waste water described in every liter, the input amount of chlorella is (5-10) × 108CFU is withered
The input amount of careless bacillus is (60-120) × 108CFU, the input amount of bacillus coagulans are (50-100) × 108CFU。
3. according to the method described in claim 2, wherein, the cultivation has in nacreous pond, nacreous cultivation density
Only for 950-1100 per acre;Relative to aquiculture waste water described in every liter, the input amount of chlorella is (6-8) × 108CFU is withered
The input amount of careless bacillus is (80-110) × 108CFU, the input amount of bacillus coagulans are (70-90) × 108CFU。
4. method according to claim 1 to 3, wherein the chlorella is with the chlorella algae solution of logarithmic growth phase
Form is added.
5. according to the method described in claim 4, wherein, the chlorella algae solution of the logarithmic growth phase is by including the following steps
Method obtain:
S1, chlorella algae strain is pressed every liter 104-105The inoculum concentration of CFU is inoculated in liquid enriched medium, in 2000-3000lux
Under intensity of illumination, at 25-30 DEG C after shaking flask culture 2-3 days, chlorella frond is harvested by centrifugation,
S2, using chlorella frond as the operation of chlorella algae strain return step S1, repeat 3-4 times, the bead after being strengthened
Algae frond;
S3, the chlorella frond after reinforcing is pressed every liter 105-106The inoculum concentration of CFU is inoculated in liquid enriched medium,
3000-4000lux intensity of illumination at 25-30 DEG C after shaking flask culture 5-6 days, is harvested by centrifugation to obtain the chlorella algae after light stimulus
Body;
S4, chlorella frond is pressed every liter 106-107The inoculum concentration of CFU is inoculated in liquid enriched medium, is added to 30 DEG C of constant temperature
In culture apparatus, cultivated under aerobic condition until cell density reaches 108CFU·mL-1, obtain the chlorella of growth logarithmic phase
Algae solution.
6. according to the method described in claim 5, wherein, the liquid enriched medium includes following component: NaNO3 1900-
2100mg/L, K2HPO40.04-0.06mg/L, citric acid 5.5-6.5mg/L.
7. method according to claim 1 to 3, wherein the chlorella is to assist source biotechnology to have purchased from strand city
The chlorella of limit company, the bacillus subtilis are that the article number for creating Biotechnology Co., Ltd purchased from Xiamen Sheng Langsai is
The bacillus subtilis of 68038-70-0, the bacillus coagulans are purchased from Wuhan far at the commodity for creating Science and Technology Ltd.
Number be 68038-65-3 bacillus coagulans.
8. according to the method described in claim 1, wherein, being added by chlorella, bacillus subtilis and bacillus coagulans
After into the breeding wastewater, the time of improvement is 30-120 days.
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CN114990018A (en) * | 2022-06-09 | 2022-09-02 | 江苏农牧科技职业学院 | Domestication method and device of bacillus subtilis for changing bottom of aquatic products |
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