CN114990018A - Domestication method and device of bacillus subtilis for changing bottom of aquatic products - Google Patents
Domestication method and device of bacillus subtilis for changing bottom of aquatic products Download PDFInfo
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- C—CHEMISTRY; METALLURGY
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/18—Flow directing inserts
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- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
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- C12M27/24—Draft tube
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention discloses a method and equipment for domesticating bacillus subtilis for improving the bottom of aquatic products, and relates to the technical field of improving the bottom of aquatic products. The bottom-changing product aims to solve the problems that the existing bottom-changing products on the market have no pertinence, the bottom-changing effect is general, specific bacillus strains can not be put in according to different pond bottom environments, and organic matters in bottom mud can not be effectively oxidized. A method and equipment for domesticating bacillus subtilis for changing bottom of aquatic products are disclosed, wherein bacillus subtilis is activated in a culture medium by preparing the culture medium, a good environment is provided for a mechanism for domesticating bacillus in aquaculture wastewater, the culture medium can achieve the effect of high-density growth of organic matters in the aquaculture wastewater, the bacillus subtilis has a specific culture effect, bacillus strains specifically adapting to the bottom environment of an aquaculture pond are obtained by gradual domestication, organic matters in bottom mud can be oxidized more effectively, the bottom environment of the pond is improved, and the pertinence is strong.
Description
Technical Field
The invention relates to the technical field of aquatic product bottom changing, in particular to a method and equipment for domesticating bacillus subtilis for aquatic product bottom changing.
Background
The ammonia nitrogen, nitrite, hydrogen sulfide and the like in the pond exceed the standard due to the oxygen deficiency at the bottom of the pond, so that the aquaculture yield is low and the output value is low. However, the existing aquatic product bottom-improving method still has the following problems:
1. the existing bottom-modifying products on the market have no pertinence, have general bottom-modifying effect, cannot carry out specific bacillus strain feeding according to different pond bottom environments, and cannot effectively oxidize organic matters in bottom mud;
2. the aquaculture wastewater contains various strains or sludge, and part of microorganisms are easily inhibited by toxic substances in the wastewater, so that the sludge or the strains in the aquaculture wastewater are required to be domesticated for a long time before adding a bottom-improving product, but the microorganisms in the aquaculture wastewater are difficult to utilize and degrade;
3. the culture medium and the simulated wastewater can not be quantitatively added in the preparation process, the process is not precisely measured, the addition amount can not be ensured, the error is large, and the domestication effect is greatly influenced due to the fact that the addition is concentrated at one position to cause uneven concentration.
Disclosure of Invention
The invention aims to provide a method and equipment for domesticating bacillus subtilis for changing aquatic products into bottom, which activate bacillus subtilis in a culture medium by preparing the culture medium, provide a good environment for a mechanism for domesticating bacillus in aquaculture wastewater further, ensure that the culture medium can achieve the effect of high-density growth of organic matters in the aquaculture wastewater, have a specific culture effect on the bacillus, gradually domesticate to obtain bacillus strains specifically adapted to the bottom environment of an aquaculture pond, are non-toxic and harmless, are environment-friendly, and can more effectively oxidize organic matters in bottom mud, thereby improving the bottom environment of the pond, and have strong pertinence so as to solve the problems provided in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
a domestication method of bacillus subtilis for improving bottom of aquatic products comprises the following steps:
s1: activating bacillus, preparing a culture medium, and activating the bacillus subtilis in the culture medium;
s2: domesticating bacillus, preparing simulated aquaculture wastewater, and gradually increasing the content of the simulated aquaculture wastewater in a culture medium;
s3: obtaining bacillus, gradually domesticating to obtain bacillus strain specifically adapted to the bottom environment of the aquaculture pond.
Further, in the step S1, the culture medium is composed of the following raw material components in parts by weight: the using amount of distilled water is 1L, the using amount of glucose is 20g, the using amount of peptone is 15g, the using amount of sodium chloride is 5g, the using amount of beef extract is 0.5g, and the using amount of agar is 20 g.
Further, for S1, the ph of the medium was 7 and the temperature was 32 ℃.
Further, in S2, the preparation of the simulated aquaculture wastewater comprises the following steps:
s201: taking f/2 culture medium as base liquid, and adding ammonium chloride and sodium dihydrogen phosphate to simulate initial PO of aquaculture wastewater 4 3- -P(NaH 2 PO 4 ) Concentration 1.5mg/L, NH 4 +-N(NH 4 Cl) concentrations were 0mg/L (f/2 medium), 20mg/L, 40mg/L, 80mg/L, 100mg/L, respectively, and COD (glucose) concentration was 500 mg/L.
Further, in S201, a trace element liquid is added into the simulated aquaculture wastewater in the preparation process, and the trace element liquid comprises the following raw material components in parts by weight: h 3 BO 3 In an amount of 2.9 x 10 -3 g/L，MnCI 2 -4H 2 The amount of O is 1.8 x 10 -5 g/L，ZnSO 4 -7H 2 The amount of O is 2.2 x 10 -4 g/L，Na 2 MoO 4 -2H 2 The amount of O is 3.9 × 10 -4 g/L，CuSO 4 -5H 2 The amount of O is 8.0 x 10 -5 g/L，Co(NO 3 ) 2 -6H 2 The amount of O is 5.0 x 10 -5 g/L。
The invention provides another technical scheme, and the device comprises an incubator for placing a culture medium and allocating simulated aquaculture wastewater, wherein the center of the upper surface of the incubator is fixedly connected with a liquid feeding pressure barrel through a bolt, the liquid feeding pressure barrel is arranged to be a transparent glass barrel, the side wall of the liquid feeding pressure barrel is provided with a scale, the lower surface of the inner wall of the incubator is fixedly connected with a culture dish through a bolt, the center of the upper surface of the inner wall of the incubator is fixedly connected with an upper liquid feeding pipe through a bolt, the lower end of the upper liquid feeding pipe is connected with a lower liquid feeding pipe in a nested manner, the periphery of the upper surface of the inner wall of the incubator is respectively fixedly connected with guide columns through bolts, the lower surface of each guide column is fixedly connected with the periphery of a supporting plate through a bolt, each guide column is arranged to be a telescopic column, the center of the supporting plate is fixedly connected with the outer wall of the lower liquid feeding pipe, and the outer wall of the culture dish is sleeved with a heating wire.
Further, lower feed liquor pipe lower surface passes through the bearing and divides the flow box to rotate to be connected, divides the flow box to rotate through bolt and reposition of redundant personnel pipe fixed connection all around respectively, divides the flow box inner wall lower surface to rotate through bearing and reposition of redundant personnel axle to be connected, divides the axle lateral wall to be provided with evenly distributed's reposition of redundant personnel flabellum respectively, and lower feed liquor pipe lower surface is provided with the liquid outlet respectively, and the liquid outlet is the slope form respectively and below feed liquor pipe lower surface centre of a circle central symmetry distributes to same direction slope setting respectively.
Furthermore, the center of the lower surface of the flow distribution box is rotatably connected with a transmission shaft through a bearing, the upper surface of the transmission shaft is fixedly connected with a flow distribution shaft, a guide shaft sleeve is sleeved on the side wall of the transmission shaft, the periphery of the guide shaft sleeve is fixedly connected with the side wall of the flow distribution guide pipe through a connecting rod respectively, the lower surface of the transmission shaft is fixedly connected with a central liquid level floating ball through a bolt, the periphery of the central liquid level floating ball is fixedly connected with a side liquid level floating ball through a floating plate, one end of the floating plate is provided with a limit groove matched with the side wall of the side liquid level floating ball respectively, and the limit groove is connected with the side liquid level floating ball in a nested buckling mode.
Furthermore, the side liquid level floating balls and the shunt pipes are respectively arranged in a staggered mode, and the lower surfaces of the shunt pipes are provided with flow guide ports which are obliquely arranged.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the domestication method and equipment of the bacillus subtilis for changing bottom of the aquatic product, provided by the invention, the bacillus subtilis is activated in the culture medium by preparing the culture medium, so that a good environment is provided for a mechanism for further domesticating the bacillus in the aquaculture wastewater, the culture medium can achieve the effect of high-density growth of organic matters in the aquaculture wastewater, the culture effect of specificity on the bacillus is achieved, bacillus strains which are specifically adapted to the bottom environment of an aquaculture pond are obtained by gradual domestication, the method and equipment are non-toxic, harmless, environment-friendly and capable of more effectively oxidizing the organic matters in bottom mud, so that the bottom environment of the pond is improved, and the pertinence is strong.
2. According to the domestication method and equipment of the bacillus subtilis for changing bottom of the aquatic product, the configured simulated aquaculture wastewater is added into the liquid adding pressure barrel, the scale is convenient to observe the adding amount of the simulated aquaculture wastewater, the quantitative adding effect is achieved, the scale is accurate, the required dosage is accurate, the adding amount precision is improved, and errors are reduced.
3. According to the domestication method and the domestication equipment of the bacillus subtilis for changing bottom of the aquatic products, simulated aquaculture wastewater is conveyed to one side of the shunting fan blades through the liquid outlet and drives the shunting shaft to rotate, so that the shunting guide pipes rotate and simultaneously convey the simulated aquaculture wastewater into the culture dish respectively, the uniformity of added wastewater is improved, the simulated aquaculture wastewater is uniformly mixed with a culture medium, the domestication effect is improved, the shunting guide pipes are adjusted according to the height of the liquid level in the culture dish, the supporting plates are vertically translated along with the shunting guide pipes, the guide columns effectively play a role in guiding, the phenomenon that the guide columns are inclined in the vertical moving process is avoided, and pollution caused by the fact that a guide port at the lower end of the shunting guide pipe is in contact with the culture medium is avoided.
Drawings
FIG. 1 is a flow chart of a method for acclimating Bacillus subtilis according to the present invention;
FIG. 2 is an isometric view of an incubator construction of the invention;
FIG. 3 is an isometric view of the internal structure of the incubator of the present invention;
FIG. 4 is a perspective view of the connecting shaft of the culture dish and the shunt tube of the present invention;
FIG. 5 is an exploded perspective view of the connection of the lower inlet pipe and the diverter box of the present invention;
fig. 6 is an isometric view of a partial structure of the present invention.
In the figure: 1. an incubator; 11. an upper liquid inlet pipe; 12. a lower liquid inlet pipe; 13. a guide post; 14. a supporting plate; 15. a liquid outlet; 2. adding a hydraulic barrel; 3. a culture dish; 31. heating wires; 4. a shunt box; 41. a shunt conduit; 42. a shunt shaft; 43. shunting fan blades; 5. a drive shaft; 51. a guide shaft sleeve; 52. a connecting rod; 53. a central liquid level float ball; 54. a floating plate; 55. a side liquid level floating ball; 56. a limiting groove.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1, a method for domesticating bacillus subtilis for improving bottom of aquatic products comprises the following steps:
s1: activating bacillus, preparing a culture medium, and activating the bacillus subtilis in the culture medium;
the culture medium comprises the following raw material components in parts by weight: the usage amount of distilled water is 1L, the usage amount of glucose is 20g, the usage amount of peptone is 15g, the usage amount of sodium chloride is 5g, the usage amount of beef extract is 0.5g, the usage amount of agar is 20g, the pH value of the culture medium is 7, the temperature is 32 ℃, the culture medium contains strain factors which have high specificity and strong representativeness to the aquaculture wastewater environment, the interference of other factors which are not related to the aquaculture wastewater environment is reduced, and a good environment is provided for a mechanism for further domesticating bacillus in the aquaculture wastewater;
s2: domesticating bacillus, preparing simulated aquaculture wastewater, and gradually increasing the content of the simulated aquaculture wastewater in a culture medium;
s201: taking f/2 culture medium as base liquid, and adding ammonium chloride and sodium dihydrogen phosphate to simulate initial PO of aquaculture wastewater 4 3- -P(NaH 2 PO 4 ) Concentration 1.5mg/L, NH 4 +-N(NH 4 Cl) concentrations were 0mg/L (f/2 medium), 20mg/L, 40mg/L, 80mg/L, 100mg/L, respectively, and COD (glucose) concentration was 500 mg/L;
the simulated aquaculture wastewater is added with a trace element liquid in the preparation process, wherein the trace element liquid comprises the following raw material components in parts by weight: h 3 BO 3 In an amount of 2.9 x 10 -3 g/L，MnCI 2 -4H 2 The amount of O is 1.8 x 10 -5 g/L，ZnSO 4 -7H 2 The amount of O is 2.2 x 10 -4 g/L，Na 2 MoO 4 -2H 2 The amount of O is 3.9 × 10 -4 g/L，CuSO 4 -5H 2 The amount of O is 8.0 x 10 -5 g/L，Co(NO 3 ) 2 -6H 2 The amount of O is 5.0 x 10 -5 g/L, the components of the simulated aquaculture wastewater added with the trace element liquid are similar to those of the current aquaculture wastewater, so that the culture medium can achieve the effect of high-density growth of organic matters in the aquaculture wastewater, controllably promote the growth of certain organic matters, study the tendencies of different organic matters to the same strain and have the specific culture effect on bacillus;
s3: the bacillus is obtained and gradually domesticated to obtain bacillus strains specifically adapted to the bottom environment of the aquaculture pond, the bacillus strains are non-toxic, harmless and environment-friendly, organic matters in the bottom mud can be more effectively oxidized, so that the bottom environment of the pond is improved, the pertinence is strong, and different bacillus strains are domesticated according to the bottom environments of different aquaculture ponds.
Referring to fig. 2-4, an apparatus for acclimatization of bacillus subtilis for bottom-changing of aquatic products comprises an incubator 1 for placing a culture medium and dispensing simulated aquaculture wastewater, the center of the upper surface of the incubator 1 is fixedly connected with a liquid feeding pressure barrel 2 through a bolt, the liquid feeding pressure barrel 2 is a transparent glass cylinder, the side wall of the liquid feeding pressure barrel 2 is provided with a scale, the lower surface of the inner wall of the incubator 1 is fixedly connected with a culture dish 3 through a bolt, the center of the upper surface of the inner wall of the incubator 1 is fixedly connected with an upper liquid feeding pipe 11 through a bolt, the lower end of the upper liquid feeding pipe 11 is nested with a lower liquid feeding pipe 12, the periphery of the upper surface of the inner wall of the incubator 1 is fixedly connected with a guide post 13 through a bolt, the lower surface of the guide post 13 is fixedly connected with the periphery of a supporting plate 14 through a bolt, the guide post 13 is a telescopic post, the center of the supporting plate 14 is fixedly connected with the outer wall of the lower liquid feeding pipe 12, a heating wire 31 is sleeved on the outer wall of the culture dish 3, the culture dish 3 is internally provided with a culture medium, the heating wire 31 effectively ensures that the temperature of liquid in the culture dish 3 is stably kept at a required temperature, the configured simulated aquaculture wastewater is added into the liquid adding pressure barrel 2, the liquid adding pressure barrel 2 pressurizes the simulated aquaculture wastewater into the upper liquid inlet pipe 11 and the lower liquid inlet pipe 12, the graduated scale is convenient for observing the adding amount of the simulated aquaculture wastewater, the effect of quantitative adding is achieved, the scales are accurate, the accuracy of the required dosage is ensured, the adding amount precision is improved, the error is reduced, the supporting plate 14 is vertically translated along with the liquid level in the culture dish 3, the guide column 13 effectively plays a role in guiding, and the inclination in the vertical moving process is avoided;
referring to fig. 5-6, the lower surface of the lower liquid inlet pipe 12 is rotatably connected with the flow distribution box 4 through a bearing, the periphery of the flow distribution box 4 is fixedly connected with the flow distribution guide pipe 41 through bolts, the lower surface of the inner wall of the flow distribution box 4 is rotatably connected with the flow distribution shaft 42 through a bearing, the side walls of the flow distribution shaft 42 are respectively provided with flow distribution fan blades 43 which are uniformly distributed, the lower surface of the lower liquid inlet pipe 12 is respectively provided with liquid outlets 15, the liquid outlets 15 are respectively inclined and are respectively arranged in the same direction in a manner of being symmetrically distributed below the center of the circle of the lower surface of the liquid inlet pipe 12, the center of the lower surface of the flow distribution box 4 is rotatably connected with the transmission shaft 5 through a bearing, the upper surface of the transmission shaft 5 is fixedly connected with the flow distribution shaft 42, the side wall of the transmission shaft 5 is sleeved with a guide shaft sleeve 51, the periphery of the guide shaft sleeve 51 is fixedly connected with the side wall of the flow distribution guide pipe 41 through a connecting rod 52, and the lower surface of the transmission shaft 5 is fixedly connected with a central liquid level floating ball 53 through a bolt, the periphery of the central liquid level floating ball 53 is fixedly connected with the side liquid level floating ball 55 through a floating plate 54, one end of the floating plate 54 is provided with a limiting groove 56 matched with the side wall of the side liquid level floating ball 55, the limiting groove 56 is connected with the side liquid level floating ball 55 in a nested and buckled manner, the side liquid level floating ball 55 and the shunt pipe 41 are arranged in a staggered manner, the lower surface of the shunt pipe 41 is provided with a flow guide port, the flow guide port is arranged in an inclined manner, the simulated aquaculture wastewater is conveyed to one side of the shunt fan blade 43 through the liquid outlet 15 and drives the shunt shaft 42 to rotate, so that the transmission shaft 5 drives the shunt pipe 41 to rotate in the culture dish 3 through the guide shaft sleeve 51, the shunt pipe 41 conveys the simulated aquaculture wastewater into the culture dish 3 while rotating, the simulated aquaculture wastewater flows into a culture medium along the inner wall of the culture dish 3, the height of the central liquid level floating ball 53, the floating plate 54 and the side liquid level floating ball 55 adjust the height of the shunt pipe 41 according to the liquid level in the culture dish 3, avoid the pollution caused by the contact of the lower diversion port of the shunt conduit 41 and the culture medium.
In conclusion, the domestication method and the domestication equipment of the bacillus subtilis for changing bottom of the aquatic product activate the bacillus subtilis in the culture medium by preparing the culture medium, provide a good environment for a mechanism for further domesticating the bacillus in the aquaculture wastewater, enable the culture medium to achieve the effect of high-density growth of organic matters in the aquaculture wastewater, have a specific culture effect on the bacillus, gradually domesticate to obtain bacillus strains specifically adapted to the bottom environment of an aquaculture pond, are non-toxic, harmless, environment-friendly, and can more effectively oxidize organic matters in bottom mud, thereby improving the bottom environment of the pond, have strong pertinence, conveniently observe the addition amount of the simulated aquaculture wastewater by adding the configured simulated aquaculture wastewater into the liquid adding pressure barrel 2, achieve the effect of quantitative addition, and have precise scales, thereby guarantee the accuracy of required dosage, improve the precision of addition, reduce the error, simulation aquaculture waste water is carried to reposition of redundant personnel flabellum 43 one side through liquid outlet 15, and drive reposition of redundant personnel axle 42 and rotate, thereby reposition of redundant personnel pipe 41 is rotating simultaneously and is carrying simulation aquaculture waste water in to culture dish 3 respectively, simulation aquaculture waste water flows in the culture medium along culture dish 3 inner wall, central liquid level floater 53, floating plate 54 and side liquid level floater 55 are according to the liquid level height adjustment reposition of redundant personnel pipe 41 height in the culture dish 3, fagging 14 carries out the up-and-down translation along with the liquid level height in the culture dish 3, guide post 13 effectively plays the effect of direction, avoid reciprocating the in-process and taking place the slope, avoid reposition of redundant personnel pipe 41 lower extreme water conservancy diversion mouth and culture medium contact to cause the pollution.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be able to cover the technical solutions and the inventive concepts of the present invention within the technical scope of the present invention.
Claims (9)
1. A domestication method of bacillus subtilis for changing bottom of aquatic products is characterized by comprising the following steps: the method comprises the following steps:
s1: activating bacillus, preparing a culture medium, and activating the bacillus subtilis in the culture medium;
s2: domesticating bacillus, preparing simulated aquaculture wastewater, and gradually increasing the content of the simulated aquaculture wastewater in a culture medium;
s3: obtaining bacillus, gradually domesticating to obtain bacillus strain specifically adapted to the bottom environment of the aquaculture pond.
2. The domestication method of bacillus subtilis for improving the bottom of aquatic products as claimed in claim 1, wherein: in S1, the culture medium consists of the following raw material components in parts by weight: the using amount of distilled water is 1L, the using amount of glucose is 20g, the using amount of peptone is 15g, the using amount of sodium chloride is 5g, the using amount of beef extract is 0.5g, and the using amount of agar is 20 g.
3. The domestication method of bacillus subtilis for improving the bottom of aquatic products as claimed in claim 2, wherein: for S1, the ph of the medium was 7 and the temperature was 32 degrees celsius.
4. The domestication method of bacillus subtilis for improving the bottom of aquatic products as claimed in claim 1, wherein: aiming at S2, the preparation of the simulated aquaculture wastewater comprises the following steps:
s201: taking f/2 culture medium as base liquid, adding ammonium chloride and sodium dihydrogen phosphate to simulate initial PO of the aquaculture wastewater 4 3- -P(NaH 2 PO 4 ) Concentration 1.5mg/L, NH 4 +-N(NH 4 Cl) concentrations were 0mg/L (f/2 medium), 20mg/L, 40mg/L, 80mg/L, 100mg/L, respectively, and COD (glucose) concentration was 500 mg/L.
5. The domestication method of bacillus subtilis for improving the bottom of aquatic products as claimed in claim 4, wherein: aiming at S201, trace element liquid is added into the simulated aquaculture wastewater in the preparation process, and the trace element liquid comprises the following raw material components in parts by weight: h 3 BO 3 In an amount of 2.9 x 10 -3 g/L，MnCI 2 -4H 2 The amount of O is 1.8 x 10 -5 g/L，ZnSO 4 -7H 2 The amount of O is 2.2 x 10 -4 g/L，Na 2 MoO 4 -2H 2 The amount of O is 3.9 x 10 -4 g/L，CuSO 4 -5H 2 The amount of O is 8.0 x 10 -5 g/L，Co(NO 3 ) 2 -6H 2 The amount of O is 5.0 x 10 -5 g/L。
6. The apparatus for acclimatization method of bacillus subtilis for aquaculture according to claim 1, comprising an incubator (1) for placing culture medium and dispensing simulated aquaculture wastewater, characterized in that: incubator (1) upper surface center passes through bolt and liquid feeding pressure bucket (2) fixed connection, liquid feeding pressure bucket (2) set up to transparent glass section of thick bamboo, liquid feeding pressure bucket (2) lateral wall is provided with the scale, incubator (1) inner wall lower surface passes through bolt and culture dish (3) fixed connection, incubator (1) inner wall upper surface center passes through bolt and last feed pipe (11) fixed connection, go up feed pipe (11) lower extreme and lower feed pipe (12) nested connection, incubator (1) inner wall upper surface is all around respectively through bolt and guide post (13) fixed connection, guide post (13) lower surface passes through bolt and fagging (14) fixed connection all around, guide post (13) set up to flexible post, fagging (14) center and lower feed pipe (12) outer wall fixed connection, culture dish (3) outer wall cover has heater strip (31).
7. The domestication method and equipment of bacillus subtilis for improving bottom of aquatic products as claimed in claim 6, wherein: lower feed liquor pipe (12) lower surface passes through the bearing and divides flow box (4) to rotate to be connected, divide flow box (4) to rotate through bolt and reposition of redundant personnel pipe (41) fixed connection all around respectively, divide flow box (4) inner wall lower surface to pass through the bearing and divide flow shaft (42) to rotate to be connected, divide flow shaft (42) lateral wall to be provided with evenly distributed's reposition of redundant personnel flabellum (43) respectively, lower feed liquor pipe (12) lower surface is provided with liquid outlet (15) respectively, liquid outlet (15) are the slope form respectively and below feed liquor pipe (12) lower surface centre of a circle central symmetry distributes and slope setting to same direction respectively.
8. The domestication method and equipment of bacillus subtilis for improving bottom of aquatic products as claimed in claim 7, wherein: the center of the lower surface of the flow distribution box (4) is rotatably connected with a transmission shaft (5) through a bearing, the upper surface of the transmission shaft (5) is fixedly connected with a flow distribution shaft (42), a guide shaft sleeve (51) is sleeved on the side wall of the transmission shaft (5), the periphery of the guide shaft sleeve (51) is fixedly connected with the side wall of a flow distribution guide pipe (41) through a connecting rod (52), the lower surface of the transmission shaft (5) is fixedly connected with a central liquid level floating ball (53) through a bolt, the periphery of the central liquid level floating ball (53) is fixedly connected with a side liquid level floating ball (55) through a floating plate (54), one end of the floating plate (54) is provided with a limiting groove (56) matched with the side wall of the side liquid level floating ball (55), and the limiting groove (56) is connected with the side liquid level floating ball (55) in a nested and buckled mode.
9. The domestication method and equipment of bacillus subtilis for improving bottom of aquatic products as claimed in claim 8, wherein: the side liquid level floating balls (55) and the shunt pipes (41) are arranged in a staggered mode respectively, and the lower surfaces of the shunt pipes (41) are provided with flow guide openings which are arranged in an inclined mode.
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