CN110226451A - A kind of edible fungus industrial formula of beanstalk stalk preparation - Google Patents
A kind of edible fungus industrial formula of beanstalk stalk preparation Download PDFInfo
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- CN110226451A CN110226451A CN201910583094.2A CN201910583094A CN110226451A CN 110226451 A CN110226451 A CN 110226451A CN 201910583094 A CN201910583094 A CN 201910583094A CN 110226451 A CN110226451 A CN 110226451A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Abstract
The invention discloses a kind of edible fungus industrial formulas of beanstalk stalk preparation, it is related to edible fungus industrial cultivation technical field, the edible fungus industrial formula of beanstalk stalk preparation, its raw material includes: 4-6 parts of beanstalk stalk by weight, 32-36 parts of corncob, 25-30 parts of rice bran, 10-14 parts of wheat bran, 4-6 parts of brewex's grains, 5-8 parts of soybean skin, 1-3 parts of conch meal, 1-3 parts of precipitated calcium carbonate, 6-8 parts of cotton seed hulls, 5-7 parts of megasse, the following steps are included: S1, raw material preparation: by beanstalk stalk, corncob, rice bran, wheat bran, brewex's grains, soybean skin, conch meal, precipitated calcium carbonate, cotton seed hulls and megasse are mixed into compost according to the ratio of regulation.The present invention is by addition beanstalk stalk as part material, it solves and is rapidly developed recently as edible fungus industrial, the problem of prices of raw materials of offer nitrogen source rise steadily and start the phenomenon for supply deficiency occur in the market, the cost of edible fungus industrial cultivation is caused integrally to increase substantially.
Description
Technical field
The present invention relates to edible fungus industrial cultivation technical field, specially a kind of edible fungus industrial of beanstalk stalk preparation
Formula.
Background technique
Edible fungus industrial cultivation refers to the environment for creating edibility bacterium different developmental phases, carries out the anti-season of scale
Annually cultivating, since annual continuous production can be reached regardless of positive anti-season with the growing environment condition of manual adjustment edible mushroom,
Fruiting everyday, whole year listing, to ensure that the steady production of edible mushroom, as the continuous development of economy and consumption demand increases
By force, the factorial production is the inexorable trend of Edible Fungi Industry Development, mainly have agaricus bisporus, needle mushroom, true pleurotus cornucopiae, Pleurotus eryngii,
The kinds such as Pleurotus nebrodensis and straw mushroom.
Edible fungus industrial cultivation mainly has that bottle is planted and bag plants two kinds of the factorial production modes, in bottle cultivation system, spice, dress
Bottle and mycelium stimulation are all made of do mechanization operation.It is built erect from compost preparation, spice, bottling, sterilizing, cooling, inoculation, culture,
Mycelium stimulation, flower bud, inhibition, fertility to a whole set of standardized production technique of harvesting.
Beanstalk stalk refers to the stem and leaf after legume crop threshing, generally referred to as soybean stalks.In rural area, soya bean threshing
After harvesting, remaining beanstalk stalk has been used as conventional fuel, causes serious influence to environment.The edible mushroom of artificial cultivation
Entity is to possess higher yield and preferable quality, and nitrogen content is had within the specified scope in culture medium, and nitrogen content is inclined
The low health degree that will affect fructification, and shorten the shelf life of edible mushroom.In order to guarantee that the quality of edible mushroom is stablized, culture
Nutriment has to good according to scientific proportions in matrix.It is rapidly developed recently as edible fungus industrial, nitrogen is provided
The price of the raw material such as the raw material in source such as wheat bran or soybean skin rises steadily and starts supply occur in the market insufficient
The cost of edible fungus industrial cultivation is greatly improved in phenomenon, factors above, constrains the development of edible fungus industrial, because
This finds high-quality, valence is low and nutrient content is high raw material when producing acupuncture needle edible mushroom for the hair of edible fungus industrial
Exhibition and progress become particularly important, and we provide a kind of edible fungus industrial formulas of beanstalk stalk preparation thus.
Summary of the invention
(1) the technical issues of solving
In view of the deficiencies of the prior art, the present invention provides a kind of edible fungus industrial formulas of beanstalk stalk preparation, solve
It is rapidly developed recently as edible fungus industrial, the prices of raw materials for providing nitrogen source rise steadily and start to occur in the market
The phenomenon of supply deficiency, the problem of causing the cost of edible fungus industrial cultivation integrally to increase substantially.
(2) technical solution
To achieve the above object, the invention provides the following technical scheme: a kind of edible fungus industrial of beanstalk stalk preparation is matched
Side, raw material include: 4-6 parts of beanstalk stalk by weight, and 32-36 parts of corncob, 25-30 parts of rice bran, 10-14 parts of wheat bran, beer
It is 4-6 parts poor, 5-8 parts of soybean skin, 1-3 parts of conch meal, 1-3 parts of precipitated calcium carbonate, 6-8 parts of cotton seed hulls, 5-7 parts of megasse.
A kind of edible fungus industrial formula of beanstalk stalk preparation, comprising the following steps:
S1, raw material preparation: by beanstalk stalk, corncob, rice bran, wheat bran, brewex's grains, soybean skin, conch meal, lightweight carbonic acid
Calcium, cotton seed hulls and megasse are mixed into compost according to the ratio of regulation.
S2, mixing: feed intake --- dry mixing --- wet-mixing, is uniformly mixed compost, detects the moisture content of compost
Control is configured to culture medium in 67-69%, pH 6.2-6.8.
S3, bottling: prepared culture medium is filled in culture bottle using fully automatic bottle filling machine, culture bottle capacity
1200ml, every bottle of charge are 960-1050g, and every 16 culture bottles are one basket.
S4, punching and closing lid: it is punched among charge level with surrounding after filling by automatic punch, altogether 5 holes
Hole, and hole extends to bottom of bottle, it is desirable that culture medium charge level is smooth, after the completion of punching, tightly cultivates bottle cap by automatic cover lid cover
Upper matched bottle cap.
S5, high pressure steam sterilization: the cultivation basket equipped with culture bottle is placed on tote cart, and push-in vacuumizes steam sterilizing
It sterilizes in device, sterilising temp is 121 DEG C, sterilization time 90min.
S6, cooling are simultaneously inoculated with: after sterilizing, force cold by culture bottle push-in cooling chamber, material temperature is reduced to 15-23
DEG C when be inoculated with, flammulina velutipes liquid strains, every bottle of inoculum concentration are accessed into culture bottle under laminar flow hood with fully automatic inoculating machine
For 25-35ml.
S7, cultural hypha: after inoculation, culture bottle is moved into culturing room and carries out cultural hypha, culture environment are as follows: room temperature
13-15 DEG C, air humidity 75-85%, be protected from light and air circulation is good, mycelia can cover with culture bottle after culture 22-24 days.
S8, mycelium stimulation: moving into mycelium stimulation workshop for the culture bottle for covering with mycelia, carries out mycelium stimulation processing, removal with automatic mycelium stimulation machine
The mycelia of charge level aging is formed simultaneously mechanical stimulus, promotes the formation and differentiation of needle mushroom fruit body primordium.
S9 plus water spray wet charge level: spraying 10-15ml water in charge level after mycelium stimulation, charge level is made to keep wet.
S10, flower bud and inhibition: moving into fertility room for culture bottle, in 15-17 DEG C of room temperature, the air humidity of 85-95%,
Flower bud processing is carried out under the conditions of the gas concentration lwevel of 3000-4000ppm, charge level can be covered with mushroom flower bud after 4-6 days, and flower bud terminates
Afterwards, illumination and the 150m of 30-50lx intensity are carried out in due course daily3The blowing of/h and 1-3m/s carries out Xanthophyll cycle and wind to mushroom bud
Inhibit, and gas concentration lwevel control is made into the growth of mushroom bud neatly, unanimously under the conditions of 3000-8000ppm, while temperature is by day
Decline, final temperature maintain 4-6 DEG C, and small mushroom bud can grow to cultivation bottleneck within 10-12 days.
S11, breeding time culture: when mushroom bud grows cultivation bottleneck 1-2cm, plastic bag mushroom piece is wrapped around bottleneck, improves piece
Gas concentration lwevel in cylinder promotes the elongation of needle mushroom stem, suitably carries out light according to mushroom uniformity to 10000ppm or more
According to intensity of illumination is the blowing of 30-50lx and 1-3m/s, carries out Xanthophyll cycle to needle mushroom and wind inhibits, make each mushroom bud of needle mushroom
Between layering reduce, growing way is consistent as far as possible.
S12, harvesting: when needle mushroom it is long to 16-17cm when, reach harvesting and require, harvested.
Preferably, in step s 2, blender is successively put into according to feeding sequence, first dry mixing 10-15 minutes, added water,
Wet-mixing is carried out after adding water, after raw material all uniformly mixes, detects the moisture content and pH value of compost.
Preferably, in step s3, culture bottle is polypropylene plastics culture bottle.
Preferably, in the step s 7, culturing room uses automatic control system, by material temperature control at 17-20 DEG C, space humidity
Control ensures spatial loop in 3000-5000ppm, while by high-efficiency air filtering in 70-80%, gas concentration lwevel control
The cleanliness in border.
Preferably, in step S10-S12, entire cultivation period about 26-28 days for giving birth to room.
(3) beneficial effect
The present invention provides a kind of beanstalk stalk preparation edible fungus industrial formula, have it is following the utility model has the advantages that
The present invention, as part material, is worked as with beanstalk stalk and corncob instead of former edible mushroom formula by addition beanstalk stalk
In part corn flour and soybean skin raw material, the processing transport price of beanstalk stalk has well below corn flour and soybean skin etc.
Effect reduces the cultivation cost of edible fungus industrial, productivity effect is improved, and beanstalk stalk is full of nutrition, wherein containing thick protein
15.5%, crude fat 2.7%, carbohydrate 45.8%, wherein the crude protein content in beanstalk leaf is up to 24%, and beanstalk stalk is
Beanstalk stalk is applied in edible fungus culturing matrix by the by-product of crops, cheap, abundance, has been reached to useless
Object recycle, preserve the ecological environment and be effectively reduced the purpose of the cultivation cost of edible fungus industrial, solve in recent years with
Edible fungus industrial rapidly develop, the prices of raw materials for providing nitrogen source rise steadily and start to occur supply deficiency in the market
The phenomenon that, the problem of causing the cost of edible fungus industrial cultivation integrally to increase substantially.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's every other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
The present invention provides a kind of technical solution: a kind of edible fungus industrial formula of beanstalk stalk preparation, raw material is by weight
It part include: 4-6 parts of beanstalk stalk, 32-36 parts of corncob, 25-30 parts of rice bran, 10-14 parts of wheat bran, 4-6 parts of brewex's grains, soybean skin 5-
8 parts, 1-3 parts of conch meal, 1-3 parts of precipitated calcium carbonate, 6-8 parts of cotton seed hulls, 5-7 parts of megasse, part raw material market price is such as
Table 1:
Table 1: part raw material market price list
A kind of edible fungus industrial formula of beanstalk stalk preparation, comprising the following steps:
S1, raw material preparation: by beanstalk stalk, corncob, rice bran, wheat bran, brewex's grains, soybean skin, conch meal, lightweight carbonic acid
Calcium, cotton seed hulls and megasse are mixed into compost according to the ratio of regulation.
S2, mixing: feed intake --- dry mixing --- wet-mixing, is uniformly mixed compost, detects the moisture content of compost
Control is configured to culture medium in 67-69%, pH 6.2-6.8.
S3, bottling: prepared culture medium is filled in culture bottle using fully automatic bottle filling machine, culture bottle capacity
1200ml, every bottle of charge are 960-1050g, and every 16 culture bottles are one basket.
S4, punching and closing lid: it is punched among charge level with surrounding after filling by automatic punch, altogether 5 holes
Hole, and hole extends to bottom of bottle, it is desirable that culture medium charge level is smooth, after the completion of punching, tightly cultivates bottle cap by automatic cover lid cover
Upper matched bottle cap.
S5, high pressure steam sterilization: the cultivation basket equipped with culture bottle is placed on tote cart, and push-in vacuumizes steam sterilizing
It sterilizes in device, sterilising temp is 121 DEG C, sterilization time 90min.
S6, cooling are simultaneously inoculated with: after sterilizing, force cold by culture bottle push-in cooling chamber, material temperature is reduced to 15-23
DEG C when be inoculated with, flammulina velutipes liquid strains, every bottle of inoculum concentration are accessed into culture bottle under laminar flow hood with fully automatic inoculating machine
For 25-35ml.
S7, cultural hypha: after inoculation, culture bottle is moved into culturing room and carries out cultural hypha, culture environment are as follows: room temperature
13-15 DEG C, air humidity 75-85%, be protected from light and air circulation is good, mycelia can cover with culture bottle after culture 22-24 days.
S8, mycelium stimulation: moving into mycelium stimulation workshop for the culture bottle for covering with mycelia, carries out mycelium stimulation processing, removal with automatic mycelium stimulation machine
The mycelia of charge level aging is formed simultaneously mechanical stimulus, promotes the formation and differentiation of needle mushroom fruit body primordium.
S9 plus water spray wet charge level: spraying 10-15ml water in charge level after mycelium stimulation, charge level is made to keep wet.
S10, flower bud and inhibition: moving into fertility room for culture bottle, in 15-17 DEG C of room temperature, the air humidity of 85-95%,
Flower bud processing is carried out under the conditions of the gas concentration lwevel of 3000-4000ppm, charge level can be covered with mushroom flower bud after 4-6 days, and flower bud terminates
Afterwards, illumination and the 150m of 30-50lx intensity are carried out in due course daily3The blowing of/h and 1-3m/s carries out Xanthophyll cycle and wind to mushroom bud
Inhibit, and gas concentration lwevel control is made into the growth of mushroom bud neatly, unanimously under the conditions of 3000-8000ppm, while temperature is by day
Decline, final temperature maintain 4-6 DEG C, and small mushroom bud can grow to cultivation bottleneck within 10-12 days.
S11, breeding time culture: when mushroom bud grows cultivation bottleneck 1-2cm, plastic bag mushroom piece is wrapped around bottleneck, improves piece
Gas concentration lwevel in cylinder promotes the elongation of needle mushroom stem, suitably carries out light according to mushroom uniformity to 10000ppm or more
According to intensity of illumination is the blowing of 30-50lx and 1-3m/s, carries out Xanthophyll cycle to needle mushroom and wind inhibits, make each mushroom bud of needle mushroom
Between layering reduce, growing way is consistent as far as possible.
S12, harvesting: when needle mushroom it is long to 16-17cm when, reach harvesting and require, harvested.
Embodiment one
A kind of edible fungus industrial formula of beanstalk stalk preparation, comprising the following steps:
S1, raw material preparation: by 4 parts of beanstalk stalk, 32 parts of corncob, 25 parts of rice bran, 10 parts of wheat bran, 4 parts of brewex's grains, soybean skin
5 parts, 1 part of conch meal, 1 part of precipitated calcium carbonate, 6 parts of cotton seed hulls, 5 parts of megasse are mixed into compost according to the ratio of regulation.
S2, mixing: feed intake --- dry mixing --- wet-mixing, is uniformly mixed compost, detects the moisture content of compost
Control is configured to culture medium in 67-69%, pH 6.2-6.8.
S3, bottling: prepared culture medium is filled in culture bottle using fully automatic bottle filling machine, culture bottle capacity
1200ml, every bottle of charge are 960-1050g, and every 16 culture bottles are one basket.
S4, punching and closing lid: it is punched among charge level with surrounding after filling by automatic punch, altogether 5 holes
Hole, and hole extends to bottom of bottle, it is desirable that culture medium charge level is smooth, after the completion of punching, tightly cultivates bottle cap by automatic cover lid cover
Upper matched bottle cap.
S5, high pressure steam sterilization: the cultivation basket equipped with culture bottle is placed on tote cart, and push-in vacuumizes steam sterilizing
It sterilizes in device, sterilising temp is 121 DEG C, sterilization time 90min.
S6, cooling are simultaneously inoculated with: after sterilizing, force cold by culture bottle push-in cooling chamber, material temperature is reduced to 15-23
DEG C when be inoculated with, flammulina velutipes liquid strains, every bottle of inoculum concentration are accessed into culture bottle under laminar flow hood with fully automatic inoculating machine
For 25-35ml.
S7, cultural hypha: after inoculation, culture bottle is moved into culturing room and carries out cultural hypha, culture environment are as follows: room temperature
13-15 DEG C, air humidity 75-85%, be protected from light and air circulation is good, mycelia can cover with culture bottle after culture 22-24 days.
S8, mycelium stimulation: moving into mycelium stimulation workshop for the culture bottle for covering with mycelia, carries out mycelium stimulation processing, removal with automatic mycelium stimulation machine
The mycelia of charge level aging is formed simultaneously mechanical stimulus, promotes the formation and differentiation of needle mushroom fruit body primordium.
S9 plus water spray wet charge level: spraying 10-15ml water in charge level after mycelium stimulation, charge level is made to keep wet.
S10, flower bud and inhibition: moving into fertility room for culture bottle, in 15-17 DEG C of room temperature, the air humidity of 85-95%,
Flower bud processing is carried out under the conditions of the gas concentration lwevel of 3000-4000ppm, charge level can be covered with mushroom flower bud after 4-6 days, and flower bud terminates
Afterwards, illumination and the 150m of 30-50lx intensity are carried out in due course daily3The blowing of/h and 1-3m/s carries out Xanthophyll cycle and wind to mushroom bud
Inhibit, and gas concentration lwevel control is made into the growth of mushroom bud neatly, unanimously under the conditions of 3000-8000ppm, while temperature is by day
Decline, final temperature maintain 4-6 DEG C, and small mushroom bud can grow to cultivation bottleneck within 10-12 days.
S11, breeding time culture: when mushroom bud grows cultivation bottleneck 1-2cm, plastic bag mushroom piece is wrapped around bottleneck, improves piece
Gas concentration lwevel in cylinder promotes the elongation of needle mushroom stem, suitably carries out light according to mushroom uniformity to 10000ppm or more
According to intensity of illumination is the blowing of 30-50lx and 1-3m/s, carries out Xanthophyll cycle to needle mushroom and wind inhibits, make each mushroom bud of needle mushroom
Between layering reduce, growing way is consistent as far as possible.
S12, harvesting: when needle mushroom it is long to 16-17cm when, reach harvesting and require, harvested.
Embodiment two
A kind of edible fungus industrial formula of beanstalk stalk preparation, comprising the following steps:
S1, raw material preparation: by 6 parts of beanstalk stalk, 32 parts of corncob, 25 parts of rice bran, 10 parts of wheat bran, 4 parts of brewex's grains, soybean skin
5 parts, 1 part of conch meal, 1 part of precipitated calcium carbonate, 6 parts of cotton seed hulls, 5 parts of megasse are mixed into compost according to the ratio of regulation.
S2, mixing: feed intake --- dry mixing --- wet-mixing, is uniformly mixed compost, detects the moisture content of compost
Control is configured to culture medium in 67-69%, pH 6.2-6.8.
S3, bottling: prepared culture medium is filled in culture bottle using fully automatic bottle filling machine, culture bottle capacity
1200ml, every bottle of charge are 960-1050g, and every 16 culture bottles are one basket.
S4, punching and closing lid: it is punched among charge level with surrounding after filling by automatic punch, altogether 5 holes
Hole, and hole extends to bottom of bottle, it is desirable that culture medium charge level is smooth, after the completion of punching, tightly cultivates bottle cap by automatic cover lid cover
Upper matched bottle cap.
S5, high pressure steam sterilization: the cultivation basket equipped with culture bottle is placed on tote cart, and push-in vacuumizes steam sterilizing
It sterilizes in device, sterilising temp is 121 DEG C, sterilization time 90min.
S6, cooling are simultaneously inoculated with: after sterilizing, force cold by culture bottle push-in cooling chamber, material temperature is reduced to 15-23
DEG C when be inoculated with, flammulina velutipes liquid strains, every bottle of inoculum concentration are accessed into culture bottle under laminar flow hood with fully automatic inoculating machine
For 25-35ml.
S7, cultural hypha: after inoculation, culture bottle is moved into culturing room and carries out cultural hypha, culture environment are as follows: room temperature
13-15 DEG C, air humidity 75-85%, be protected from light and air circulation is good, mycelia can cover with culture bottle after culture 22-24 days.
S8, mycelium stimulation: moving into mycelium stimulation workshop for the culture bottle for covering with mycelia, carries out mycelium stimulation processing, removal with automatic mycelium stimulation machine
The mycelia of charge level aging is formed simultaneously mechanical stimulus, promotes the formation and differentiation of needle mushroom fruit body primordium.
S9 plus water spray wet charge level: spraying 10-15ml water in charge level after mycelium stimulation, charge level is made to keep wet.
S10, flower bud and inhibition: moving into fertility room for culture bottle, in 15-17 DEG C of room temperature, the air humidity of 85-95%,
Flower bud processing is carried out under the conditions of the gas concentration lwevel of 3000-4000ppm, charge level can be covered with mushroom flower bud after 4-6 days, and flower bud terminates
Afterwards, illumination and the 150m of 30-50lx intensity are carried out in due course daily3The blowing of/h and 1-3m/s carries out Xanthophyll cycle and wind to mushroom bud
Inhibit, and gas concentration lwevel control is made into the growth of mushroom bud neatly, unanimously under the conditions of 3000-8000ppm, while temperature is by day
Decline, final temperature maintain 4-6 DEG C, and small mushroom bud can grow to cultivation bottleneck within 10-12 days.
S11, breeding time culture: when mushroom bud grows cultivation bottleneck 1-2cm, plastic bag mushroom piece is wrapped around bottleneck, improves piece
Gas concentration lwevel in cylinder promotes the elongation of needle mushroom stem, suitably carries out light according to mushroom uniformity to 10000ppm or more
According to intensity of illumination is the blowing of 30-50lx and 1-3m/s, carries out Xanthophyll cycle to needle mushroom and wind inhibits, make each mushroom bud of needle mushroom
Between layering reduce, growing way is consistent as far as possible.
S12, harvesting: when needle mushroom it is long to 16-17cm when, reach harvesting and require, harvested.
Embodiment three
A kind of edible fungus industrial formula of beanstalk stalk preparation, comprising the following steps:
S1, raw material preparation: by 6 parts of beanstalk stalk, 32 parts of corncob, 25 parts of rice bran, 10 parts of wheat bran, 4 parts of brewex's grains, soybean skin
5 parts, 2 parts of conch meal, 2 parts of precipitated calcium carbonate, 6 parts of cotton seed hulls, 6 parts of megasse are mixed into compost according to the ratio of regulation.
S2, mixing: feed intake --- dry mixing --- wet-mixing, is uniformly mixed compost, detects the moisture content of compost
Control is configured to culture medium in 67-69%, pH 6.2-6.8.
S3, bottling: prepared culture medium is filled in culture bottle using fully automatic bottle filling machine, culture bottle capacity
1200ml, every bottle of charge are 960-1050g, and every 16 culture bottles are one basket.
S4, punching and closing lid: it is punched among charge level with surrounding after filling by automatic punch, altogether 5 holes
Hole, and hole extends to bottom of bottle, it is desirable that culture medium charge level is smooth, after the completion of punching, tightly cultivates bottle cap by automatic cover lid cover
Upper matched bottle cap.
S5, high pressure steam sterilization: the cultivation basket equipped with culture bottle is placed on tote cart, and push-in vacuumizes steam sterilizing
It sterilizes in device, sterilising temp is 121 DEG C, sterilization time 90min.
S6, cooling are simultaneously inoculated with: after sterilizing, force cold by culture bottle push-in cooling chamber, material temperature is reduced to 15-23
DEG C when be inoculated with, flammulina velutipes liquid strains, every bottle of inoculum concentration are accessed into culture bottle under laminar flow hood with fully automatic inoculating machine
For 25-35ml.
S7, cultural hypha: after inoculation, culture bottle is moved into culturing room and carries out cultural hypha, culture environment are as follows: room temperature
13-15 DEG C, air humidity 75-85%, be protected from light and air circulation is good, mycelia can cover with culture bottle after culture 22-24 days.
S8, mycelium stimulation: moving into mycelium stimulation workshop for the culture bottle for covering with mycelia, carries out mycelium stimulation processing, removal with automatic mycelium stimulation machine
The mycelia of charge level aging is formed simultaneously mechanical stimulus, promotes the formation and differentiation of needle mushroom fruit body primordium.
S9 plus water spray wet charge level: spraying 10-15ml water in charge level after mycelium stimulation, charge level is made to keep wet.
S10, flower bud and inhibition: moving into fertility room for culture bottle, in 15-17 DEG C of room temperature, the air humidity of 85-95%,
Flower bud processing is carried out under the conditions of the gas concentration lwevel of 3000-4000ppm, charge level can be covered with mushroom flower bud after 4-6 days, and flower bud terminates
Afterwards, illumination and the 150m of 30-50lx intensity are carried out in due course daily3The blowing of/h and 1-3m/s carries out Xanthophyll cycle and wind to mushroom bud
Inhibit, and gas concentration lwevel control is made into the growth of mushroom bud neatly, unanimously under the conditions of 3000-8000ppm, while temperature is by day
Decline, final temperature maintain 4-6 DEG C, and small mushroom bud can grow to cultivation bottleneck within 10-12 days.
S11, breeding time culture: when mushroom bud grows cultivation bottleneck 1-2cm, plastic bag mushroom piece is wrapped around bottleneck, improves piece
Gas concentration lwevel in cylinder promotes the elongation of needle mushroom stem, suitably carries out light according to mushroom uniformity to 10000ppm or more
According to intensity of illumination is the blowing of 30-50lx and 1-3m/s, carries out Xanthophyll cycle to needle mushroom and wind inhibits, make each mushroom bud of needle mushroom
Between layering reduce, growing way is consistent as far as possible.
S12, harvesting: when needle mushroom it is long to 16-17cm when, reach harvesting and require, harvested.
As a kind of technical optimization scheme of the invention, in step s 2, blender is successively put into according to feeding sequence, first
It dry mixing 10-15 minutes, adds water, carries out wet-mixing after adding water, after raw material all uniformly mixes, detect containing for compost
Water rate and pH value.
As a kind of technical optimization scheme of the invention, in step s3, culture bottle is polypropylene plastics culture bottle.
As a kind of technical optimization scheme of the invention, in the step s 7, culturing room uses automatic control system, by material temperature
Control is at 17-20 DEG C, and in 70-80%, gas concentration lwevel is controlled in 3000-5000ppm, while passing through height for space humidity control
Air filtration is imitated to ensure the cleanliness of space environment.
As a kind of technical optimization scheme of the invention, in step S10-S12, the entire cultivation period for giving birth to room is about
26-28 days.
It can to sum up obtain, the present invention, as part material, is solved recently as edible mushroom factory by addition beanstalk stalk
Change and rapidly develop, the prices of raw materials for providing nitrogen source rise steadily and start the phenomenon for supply deficiency occur in the market, cause
The problem of cost of edible fungus industrial cultivation integrally increases substantially.
It should be noted that, in this document, such as the terms "include", "comprise" or its any other variant are intended to
Non-exclusive inclusion, so that the process, method, article or equipment including a series of elements is not only wanted including those
Element, but also including other elements that are not explicitly listed, or further include for this process, method, article or equipment
Intrinsic element.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (5)
1. a kind of edible fungus industrial formula of beanstalk stalk preparation, it is characterised in that: its raw material includes: beanstalk stalk 4- by weight
6 parts, 32-36 parts of corncob, 25-30 parts of rice bran, 10-14 parts of wheat bran, 4-6 parts of brewex's grains, 5-8 parts of soybean skin, conch meal 1-3
Part, 1-3 parts of precipitated calcium carbonate, 6-8 parts of cotton seed hulls, 5-7 parts of megasse；
A kind of edible fungus industrial formula of beanstalk stalk preparation, it is characterised in that: the following steps are included:
S1, raw material preparation: by beanstalk stalk, corncob, rice bran, wheat bran, brewex's grains, soybean skin, conch meal, precipitated calcium carbonate, cotton
Seed shell and megasse are mixed into compost according to the ratio of regulation；
S2, mixing: feed intake --- dry mixing --- wet-mixing, is uniformly mixed compost, detects the moisture control of compost
Culture medium is configured in 67-69%, pH 6.2-6.8；
Prepared culture medium: being filled in culture bottle, culture bottle capacity 1200ml by S3, bottling using fully automatic bottle filling machine,
Every bottle of charge is 960-1050g, and every 16 culture bottles are one basket；
S4, punching and closing lid: being punched among charge level with surrounding after filling by automatic punch, altogether 5 holes, and
Hole extends to bottom of bottle, it is desirable that culture medium charge level is smooth, after the completion of punching, covers matching by the tight culture bottle of automatic cover lid cover
Bottle cap；
S5, high pressure steam sterilization: the cultivation basket equipped with culture bottle is placed on tote cart, and push-in vacuumizes in steam sterilizer
It sterilizes, sterilising temp is 121 DEG C, sterilization time 90min；
S6, cooling are simultaneously inoculated with: after sterilizing, by culture bottle push-in cooling chamber force it is cold, when material temperature is reduced to 15-23 DEG C
It is inoculated with, flammulina velutipes liquid strains is accessed into culture bottle under laminar flow hood with fully automatic inoculating machine, every bottle of inoculum concentration is 25-
35ml；
S7, cultural hypha: after inoculation, culture bottle is moved into culturing room and carries out cultural hypha, culture environment are as follows: room temperature 13-
15 DEG C, air humidity 75-85%, be protected from light and air circulation is good, mycelia can cover with culture bottle after culture 22-24 days；
S8, mycelium stimulation: the culture bottle for covering with mycelia is moved into mycelium stimulation workshop, mycelium stimulation processing is carried out with automatic mycelium stimulation machine, removes charge level
The mycelia of aging is formed simultaneously mechanical stimulus, promotes the formation and differentiation of needle mushroom fruit body primordium；
S9 plus water spray wet charge level: spraying 10-15ml water in charge level after mycelium stimulation, charge level is made to keep wet；
S10, flower bud and inhibition: culture bottle is moved into fertility room, in 15-17 DEG C of room temperature, the air humidity of 85-95%, 3000-
Flower bud processing is carried out under the conditions of the gas concentration lwevel of 4000ppm, charge level can be covered with mushroom flower bud after 4-6 days, after flower bud, daily
Illumination and the 150m of 30-50lx intensity are carried out in due course3The blowing of/h and 1-3m/s carries out Xanthophyll cycle to mushroom bud and wind inhibits, and
It is neat, consistent that gas concentration lwevel control is made into the growth of mushroom bud under the conditions of 3000-8000ppm, while temperature declines by day, most
Finishing temperature maintains 4-6 DEG C, and small mushroom bud can grow to cultivation bottleneck within 10-12 days；
S11, breeding time culture: when mushroom bud grows cultivation bottleneck 1-2cm, plastic bag mushroom piece is wrapped around bottleneck, is improved in piece cylinder
Gas concentration lwevel to 10000ppm or more, promote the elongation of needle mushroom stem, illumination, light suitably carried out according to mushroom uniformity
The blowing for being 30-50lx and 1-3m/s according to intensity carries out Xanthophyll cycle to needle mushroom and wind inhibits, and makes point between each mushroom bud of needle mushroom
Layer is reduced, and growing way is consistent as far as possible；
S12, harvesting: when needle mushroom it is long to 16-17cm when, reach harvesting and require, harvested.
2. a kind of edible fungus industrial formula of beanstalk stalk preparation according to claim 1, it is characterised in that: in step S2
In, blender is successively put into according to feeding sequence, first dry mixing 10-15 minutes, adds water, wet-mixing is carried out after adding water, to original
After material all uniformly mixes, the moisture content and pH value of compost are detected.
3. a kind of edible fungus industrial formula of beanstalk stalk preparation according to claim 1, it is characterised in that: in step S3
In, culture bottle is polypropylene plastics culture bottle.
4. a kind of edible fungus industrial formula of beanstalk stalk preparation according to claim 1, it is characterised in that: in step S7
In, culturing room uses automatic control system, and by material temperature control at 17-20 DEG C, space humidity is controlled in 70-80%, carbon dioxide
Concentration is controlled in 3000-5000ppm, while ensuring the cleanliness of space environment by high-efficiency air filtering.
5. a kind of edible fungus industrial formula of beanstalk stalk preparation according to claim 1, it is characterised in that: in step
In S10-S12, entire cultivation period about 26-28 days for giving birth to room.
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| CN110679390A (en) * | 2019-09-16 | 2020-01-14 | 江苏华绿生物科技股份有限公司 | Needle mushroom composite culture medium prepared from soybean dietary fiber powder and application thereof |
| CN111296173A (en) * | 2020-03-27 | 2020-06-19 | 江苏华绿生物科技股份有限公司 | Industrialized cultivation medium for velvet antler mushroom and method for cultivating edible fungi |
| CN111480514A (en) * | 2020-05-08 | 2020-08-04 | 江苏华绿生物科技股份有限公司 | Culture medium for industrial cultivation of hypsizigus marmoreus |
| CN111492895A (en) * | 2020-04-24 | 2020-08-07 | 江苏华绿生物科技股份有限公司 | Poplar sawdust composite culture medium suitable for hypsizigus marmoreus planting and preparation method thereof |
| CN111527992A (en) * | 2020-05-06 | 2020-08-14 | 江苏华绿生物科技股份有限公司 | Culture medium for hypsizigus marmoreus |
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