CN110208511B - Elastography instrument and visualized elastography equipment - Google Patents
Elastography instrument and visualized elastography equipment Download PDFInfo
- Publication number
- CN110208511B CN110208511B CN201910623306.5A CN201910623306A CN110208511B CN 110208511 B CN110208511 B CN 110208511B CN 201910623306 A CN201910623306 A CN 201910623306A CN 110208511 B CN110208511 B CN 110208511B
- Authority
- CN
- China
- Prior art keywords
- sample
- unit
- sample cup
- cup
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000002091 elastography Methods 0.000 title claims description 46
- 238000012360 testing method Methods 0.000 claims abstract description 83
- 239000007788 liquid Substances 0.000 claims abstract description 62
- 235000014676 Phragmites communis Nutrition 0.000 claims abstract description 50
- 238000010168 coupling process Methods 0.000 claims abstract description 50
- 238000005859 coupling reaction Methods 0.000 claims abstract description 50
- 230000008878 coupling Effects 0.000 claims abstract description 49
- 239000003153 chemical reaction reagent Substances 0.000 claims description 43
- 239000008280 blood Substances 0.000 claims description 15
- 210000004369 blood Anatomy 0.000 claims description 15
- 238000010438 heat treatment Methods 0.000 claims description 11
- 238000011534 incubation Methods 0.000 claims description 11
- 238000005259 measurement Methods 0.000 claims description 10
- 230000023555 blood coagulation Effects 0.000 claims description 9
- 206010053567 Coagulopathies Diseases 0.000 claims description 7
- 230000035602 clotting Effects 0.000 claims description 7
- 238000012546 transfer Methods 0.000 claims description 5
- 229910000906 Bronze Inorganic materials 0.000 claims description 4
- 239000010974 bronze Substances 0.000 claims description 4
- KUNSUQLRTQLHQQ-UHFFFAOYSA-N copper tin Chemical compound [Cu].[Sn] KUNSUQLRTQLHQQ-UHFFFAOYSA-N 0.000 claims description 4
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 2
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 230000000007 visual effect Effects 0.000 claims description 2
- 239000012530 fluid Substances 0.000 claims 1
- 238000010586 diagram Methods 0.000 abstract description 14
- 238000001514 detection method Methods 0.000 abstract description 12
- 239000000523 sample Substances 0.000 description 162
- 238000000034 method Methods 0.000 description 46
- 230000008569 process Effects 0.000 description 16
- 230000015271 coagulation Effects 0.000 description 10
- 238000005345 coagulation Methods 0.000 description 10
- 230000007246 mechanism Effects 0.000 description 8
- 208000007536 Thrombosis Diseases 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- 230000003287 optical effect Effects 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 6
- 230000008859 change Effects 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 238000004082 amperometric method Methods 0.000 description 3
- 239000011324 bead Substances 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 102000009123 Fibrin Human genes 0.000 description 2
- 108010073385 Fibrin Proteins 0.000 description 2
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 239000000306 component Substances 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 229950003499 fibrin Drugs 0.000 description 2
- 230000020764 fibrinolysis Effects 0.000 description 2
- 230000023597 hemostasis Effects 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 102000008946 Fibrinogen Human genes 0.000 description 1
- 108010049003 Fibrinogen Proteins 0.000 description 1
- 206010018910 Haemolysis Diseases 0.000 description 1
- 230000005355 Hall effect Effects 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 206010023126 Jaundice Diseases 0.000 description 1
- 206010051077 Post procedural haemorrhage Diseases 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 229940127218 antiplatelet drug Drugs 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000002306 biochemical method Methods 0.000 description 1
- 210000001268 chyle Anatomy 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 239000008358 core component Substances 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000008157 edible vegetable oil Substances 0.000 description 1
- 230000005489 elastic deformation Effects 0.000 description 1
- 229940012952 fibrinogen Drugs 0.000 description 1
- 239000003502 gasoline Substances 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 230000000951 immunodiffusion Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000010705 motor oil Substances 0.000 description 1
- 238000004848 nephelometry Methods 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 239000000106 platelet aggregation inhibitor Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000004879 turbidimetry Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
Abstract
The invention provides an elasticity chart instrument and visualized elasticity chart equipment, and relates to the technical field of medical detection, wherein the elasticity chart instrument comprises a coupling reed, an elastic element, a deformation measuring unit, a sample cup vibration unit and a controller; the controller is connected with the deformation measuring unit; one end of the coupling reed is connected with the elastic element, and the other end of the coupling reed is immersed into the liquid to be detected; the sample cup vibration unit is used for driving the sample cup filled with the liquid to be tested to vibrate; the deformation measuring unit is used for measuring the deformation amount of the elastic element when the sample cup vibrates; the controller is used for calculating and obtaining the elastic force value of the liquid to be measured according to the deformation amount. The elastic diagram instrument provided by the embodiment of the invention can improve the automation degree of the elastic diagram test, reduce the influence of human factors, does not need to carry out level adjustment treatment on the test instrument, is not easily influenced by vibration interference, and further improves the stability and accuracy of test data.
Description
Technical Field
The invention relates to the technical field of medical detection, in particular to an elastography instrument and visualized elastography equipment.
Background
The blood coagulation detection is of great clinical significance, and the thromboelastography is used as a blood coagulation detection device for evaluating the coagulation and fibrinolysis states, risks and the like of patients.
At present, more than 95% of the devices for measuring thrombus elasticity on the market use a viscosity method, which measures the coagulation function according to the viscosity change during the coagulation of a blood sample. However, thromboelastography, measured by the viscosimetry method, also has the following drawbacks:
(1) The test process needs manual operation, and a sample cup, a reagent and a sample are added manually, so that the operation process is complex and is easily influenced by human factors;
(2) The suspension wire is easily interfered by external vibration in the measuring process, and the vibration of the ground and the desktop can cause jump of the measured data, so that the stability of the measured data is poor;
(3) When the instrument is used, the instrument needs to be subjected to level adjustment treatment so as to keep the instrument in a level state, otherwise, the suspension wire easily touches the taper sleeve in the measuring process, and the accuracy of measuring data is further affected.
Disclosure of Invention
Therefore, the invention aims to provide the elasticity chart instrument and the visualized elasticity chart equipment, which can improve the automation degree of the elasticity chart test, reduce the influence of human factors, do not need to carry out level adjustment treatment on the testing instrument, are not easy to be influenced by vibration interference, and further improve the stability and the accuracy of test data.
In a first aspect, an embodiment of the present invention provides an elastography apparatus, including: the device comprises a coupling reed, an elastic element, a deformation measuring unit, a sample cup vibration unit and a controller; the controller is connected with the deformation measuring unit; one end of the coupling reed is connected with the elastic element, and the other end of the coupling reed is immersed into the liquid to be detected; the sample cup vibration unit is used for driving the sample cup filled with the liquid to be tested to vibrate; the deformation measuring unit is used for measuring the deformation amount of the elastic element when the sample cup vibrates; the controller is used for calculating and obtaining the elastic force value of the liquid to be measured according to the deformation amount.
With reference to the first aspect, an embodiment of the present invention provides a first possible implementation manner of the first aspect, wherein the deformation measurement unit includes a hall sensor or a photoelectric sensor.
With reference to the first aspect, the embodiment of the present invention provides a second possible implementation manner of the first aspect, wherein the elastic element includes an elastic rod, an elastic wire or an elastic sheet.
With reference to the first aspect, an embodiment of the present invention provides a third possible implementation manner of the first aspect, where the elastography apparatus further includes: a sample adding device; the sample adding device comprises a sample adding needle and a mechanical arm unit; the mechanical arm unit is connected with the controller, and the sample adding needle is arranged on the mechanical arm unit; the mechanical arm unit is used for moving the sample adding needle to the position of the sample cup so as to add the liquid to be measured into the sample cup through the sample adding needle.
With reference to the third possible implementation manner of the first aspect, an embodiment of the present invention provides a fourth possible implementation manner of the first aspect, where the elastography apparatus further includes: an incubation device; the incubation device comprises a first cup placing groove and a heating unit; the first sample cup placing groove is arranged on the sample cup vibrating unit; the first sample cup placing groove is used for placing a sample cup containing liquid to be tested; the heating unit is used for heating the sample cup so as to maintain the constant temperature of the liquid to be measured.
With reference to the third possible implementation manner of the first aspect, an embodiment of the present invention provides a fifth possible implementation manner of the first aspect, where the elastography apparatus further includes: a sample cup supply device; the sample cup supply device comprises: a second sample cup placing groove and a cup feeding unit; the second sample cup placing groove is used for placing an empty sample cup; the cup feeding unit is used for acquiring an empty sample cup from the second sample cup placing groove and placing the empty sample cup at a first preset position.
With reference to the fifth possible implementation manner of the first aspect, an embodiment of the present invention provides a sixth possible implementation manner of the first aspect, where the elastography apparatus further includes: a sample cup transfer device; this sample cup conveyer includes: a sample cup gripping unit and a conveying guide rail; the sample cup grabbing unit is movably arranged on the conveying guide rail; the sample cup grabbing unit is used for grabbing a sample cup from a first preset position; the conveying guide rail is used for conveying the sample cups grabbed by the sample cup grabbing unit to a second preset position.
With reference to the first aspect and one of the first to fifth possible implementation manners of the first aspect, an embodiment of the present invention provides a seventh possible implementation manner of the first aspect, wherein the liquid to be tested is a mixed liquid of a blood sample and a coagulation reagent.
With reference to the seventh possible implementation manner of the first aspect, an embodiment of the present invention provides an eighth possible implementation manner of the first aspect, wherein the elastography apparatus further includes: sample and reagent supply means; the sample and reagent supply device includes: test tube storage disc, rotation unit and code scanning unit; a sample test tube and a reagent test tube are placed on the test tube storage tray, a blood sample is placed in the sample test tube, a blood coagulation reagent is placed in the reagent test tube, and corresponding identification codes are arranged on the sample test tube and the reagent test tube; the code scanning unit is arranged at one side of the test tube storage disc; the rotating unit is used for driving the test tube storage disc to do rotating motion so as to rotate different test tubes into the scanning range of the code scanning unit; the code scanning unit is used for scanning and reading the identification codes in the scanning range so as to acquire the information of the current test tube.
In a second aspect, an embodiment of the present invention further provides a visualized elastography apparatus, including the elastography apparatus provided in one of the first aspect and its possible embodiments, and further including a display; the display is connected with the controller; the display is used for displaying the elasticity value of the liquid to be tested.
The embodiment of the invention has the following beneficial effects:
the embodiment of the invention provides an elasticity chart instrument and visualized elasticity chart equipment, wherein the elasticity chart instrument comprises a coupling reed, an elastic element, a deformation measuring unit, a sample cup vibration unit and a controller; the controller is connected with the deformation measuring unit; one end of the coupling reed is connected with the elastic element, and the other end of the coupling reed is immersed into the liquid to be detected; the sample cup vibration unit is used for driving the sample cup filled with the liquid to be tested to vibrate; the deformation measuring unit is used for measuring the deformation amount of the elastic element when the sample cup vibrates; the controller is used for calculating and obtaining the elastic force value of the liquid to be measured according to the deformation amount. The elastic force graph instrument replaces a torsion wire in a conventional elastic force graph instrument with a coupling reed, calculates the elastic force value of the liquid to be measured by measuring the vibration amplitude of the coupling reed, is different from a mode of calculating the elastic force value of the liquid to be measured by measuring the rotation angle of the torsion wire in a conventional test instrument, is a brand new measurement mode, does not need to carry out leveling treatment on the instrument before testing, and can effectively reduce the influence of vibration interference. And moreover, through setting up mechanical control structure, make the acquisition of appearance cup, add the sample, convey appearance cup, incubate to the test, whole process realizes the automation, improves the degree of automation of elasticity diagram test, has reduced the influence of human factor, and then improves test data's stability and accuracy.
Additional features and advantages of the disclosure will be set forth in the description which follows, or in part will be obvious from the description, or may be learned by practice of the techniques of the disclosure.
The foregoing objects, features and advantages of the disclosure will be more readily apparent from the following detailed description of the preferred embodiments taken in conjunction with the accompanying drawings.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present invention, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of an elastography apparatus according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of another elastography machine according to an embodiment of the present invention;
FIG. 3 is a schematic diagram of the correspondence relationship between the measured parameters of the elastography apparatus and the TEG5000 apparatus provided by the invention;
fig. 4 is a schematic structural diagram of a visual elastic diagram device according to an embodiment of the present invention.
Icon: 10-coupling a reed; 11-an elastic element; 12-a deformation measurement unit; 13-a sample cup vibration unit; 14-a controller; 15-liquid to be measured; 16-sample cup; 21-a sample cup supply; 22-sample cup transfer means; 23-sample and reagent supply means; 24-sample adding device; 25-incubation means; 26-measuring means; 41-elastography; 42-a display; 100-visualizing elastography device.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The blood coagulation detection has important clinical significance, and the thromboelastography instrument is used as blood coagulation detection equipment and has the following functions: assessing the state and risk of coagulation and fibrinolysis in a patient; monitoring the influence of anticoagulant drugs such as heparin; guiding preoperative preparation, monitoring blood coagulation dynamic in the whole process, and analyzing the cause of postoperative bleeding; guiding blood transfusion; treatment with antiplatelet agents was evaluated.
Among the methodologies for detecting coagulation are immunological methods, substrate chromogenic methods and clotting methods. The coagulation method is also classified into an amperometric method, an optical method and a magnetic bead method (also called a viscosity method). The immunological method is to take a solid-color detected substance as an antigen, prepare an antibody, and then react by using the antigen and the antibody, and is commonly used as an immunodiffusion method, an arrow electrophoresis method, an ELLSA method and an immunoturbidimetry method. The immune diffusion method has long test time and low sensitivity; the arrow electrophoresis method is complex in operation and is not suitable for clinical application; the ELLSA method is mainly suitable for clinical hemostasis and thrombus component detection; immunoturbidimetry reagents are expensive. Substrate chromogenic methods, which can also be referred to as biochemical methods and are used clinically mainly for hemostasis and thrombus constituent detection, infer the content and activity of the substance being measured by measuring the change in absorbance of a colored substrate.
The coagulation method includes an amperometric method, an optical method (nephelometry and turbidimetry by projection), and a magnetic bead method (also called viscosity method). Blood forms fibrinogen and fibrin during clotting, which is electrically conductive, and amperometric tests are based on this irrational feature. However, the amperometric method is poor in reliability and single, and is gradually replaced by the optical method and the magnetic bead method. Optical methods are based on the detection of changes in the absorption and scattering of light caused by changes in the turbidity of the blood sample during plasma coagulation. The detection result of the optical method is easily affected by factors such as optical abnormality of the sample (e.g. jaundice, chyle, hemolysis, turbidity, etc.), the finish of the test cup, bubbles generated during sample addition, and the like.
The majority of the current measurements of thrombus elasticity on the market use the viscosity method, which measures the clotting function based on the change in viscosity during the clotting process of a blood sample. A group of driving devices are arranged on two sides of the test cup, the test cup rotates left and right at a fixed frequency, fibrin is continuously increased in the blood coagulation process, the viscosity of a blood sample is increased, a cup cover placed in the sample drives a probe to rotate left and right, and an instrument detects the blood sample by detecting the motion state of the probe. It is based on this principle (viscosity method) that TEG5000, a major market holder of thromboelastography, HAEMONETICS, usa. Long-term researches show that the stability of the traditional instrument is poor, and a great part of reasons are that the detection core component is twisted, the processing diameter of the twisted wire is between 0.2mm and 0.4mm, the stability of the testing state is kept only by gravity, and in the vertical movement process, the suspended wire is disturbed by the horizontal effect and the surrounding environment.
In general, the thromboelastography apparatus measured by the viscosity method in the prior art has the problems that the level adjustment is needed when in use, and the testing process is easily affected by human factors and easily interfered by external vibration, so that the stability of the testing data is poor and the accuracy is not high.
Based on the above, the elasticity chart instrument and the visualized elasticity chart equipment provided by the embodiment of the invention can improve the stability and the accuracy of test data.
For the convenience of understanding the present embodiment, an elastography apparatus disclosed in the present embodiment will be described in detail.
Embodiment one:
fig. 1 is a schematic structural diagram of an elastography machine according to an embodiment of the present invention, and as can be seen from fig. 1, the elastography machine includes a coupling reed 10, an elastic element 11, a deformation measuring unit 12, a sample cup vibrating unit 13, and a controller 14. Wherein the controller 14 is connected to the deformation measuring unit 12, and one end of the coupling reed 10 is connected to the elastic member 11, and the other end of the coupling reed 10 is immersed in the liquid 15 to be measured.
Here, the liquid 15 to be measured is a liquid for preparing to measure its elastic force, and is usually a liquid having a certain viscoelasticity, and may be, for example, oils (edible oil, gasoline, engine oil, etc.), aqueous solutions, gels, etc.
In actual operation, the liquid 15 to be measured is contained in the sample cup 16, and the material of the sample cup 16 may be glass, plastic, etc. And, a cuvette 16 containing a liquid 15 to be measured is placed on the cuvette shaking unit 13. In one embodiment, the vibratory unit is provided with a fastener for securing the cuvette 16.
The coupling reed 10 is a spring sheet, which is easy to bend in one direction, namely the minimum rigidity plane, and has large tensile rigidity and bending rigidity in the other direction, and the coupling reed 10 is less susceptible to vibration interference than torsion wires, wherein the material of the coupling reed can be phosphor bronze, tin bronze and the like.
When the test is started, the sample cup vibration unit 13 drives the sample cup 16 containing the liquid 15 to be tested to vibrate, wherein the vibration mode can be to-and-fro motion along a certain direction on the horizontal plane, for example, back and forth reciprocating motion, and a certain frequency is reached, so that the vibration effect is realized. When the sample cup 16 vibrates, the liquid 15 to be measured in the sample cup 16 is driven to vibrate, and a viscous force exists between the liquid 15 to be measured and the coupling reed 10 due to the fact that the liquid 15 to be measured is in contact with a part of the coupling reed 10. If the viscoelasticity of the liquid 15 to be measured is larger, the viscous force between the liquid 15 to be measured and the coupling reed 10 is larger, and accordingly, the deformation amount of the coupling reed 10 is larger.
In the elastograph, the elastic element 11 acts to receive and transmit vertical loads, and to alleviate and suppress impacts caused by uneven road surfaces. The elastic element 11 has two basic effects in operation: elastic effects, which refer to the properties exhibited by the elastic element 11 due to the influence of load alone, and inelastic effects, whose specific parameters are stiffness and sensitivity to manifest load and deformation. In at least one possible embodiment, the elastic element 11 may be an elastic rod, an elastic wire or an elastic sheet. In the present embodiment, the elastic element 11 is connected to one end of the coupling reed 10, and the deformation of the coupling reed 10 is transmitted to the elastic element 11, so that the deformation amount of the coupling reed 10 is obtained by measuring the deformation amount of the elastic element 11.
In addition, the deformation measuring unit 12 is a device for measuring deformation amount, and the deformation measuring unit 12 measures deformation amount of the elastic member 11 when the cuvette shaking unit 13 shakes the cuvette 16 containing the liquid 15 to be measured. In one possible embodiment, the deformation measuring unit 12 may be a hall sensor, where the hall sensor is a magnetic field sensor made according to the hall effect; in another possible embodiment, the deformation measuring unit 12 may also be a photoelectric sensor, which is a device that converts an optical signal into an electrical signal. After the deformation measuring unit 12 measures the deformation data, the controller 14 obtains the deformation data, and obtains the elastic force value of the liquid 15 to be measured according to the deformation data.
In this way, the elastic force diagram instrument provided by the invention replaces the torsion wire in the conventional elastic force diagram instrument with the coupling reed 10, and calculates the elastic force value of the liquid 15 to be measured by measuring the vibration amplitude of the coupling reed 10, which is different from the way of calculating the elastic force value of the liquid 15 to be measured by measuring the rotation angle of the torsion wire in the conventional test instrument. In addition, the coupling reed 10 is not easy to be interfered by vibration, and the problem that a taper sleeve is encountered in the measuring process does not exist, so that the elastic force graph instrument does not need to carry out leveling treatment on the instrument before testing, and the influence of vibration interference can be effectively reduced.
The elastography instrument provided by the embodiment of the invention comprises a coupling reed, an elastic element, a deformation measuring unit, a sample cup vibration unit and a controller; the controller is connected with the deformation measuring unit; one end of the coupling reed is connected with the elastic element, and the other end of the coupling reed is immersed into the liquid to be detected; the sample cup vibration unit is used for driving the sample cup filled with the liquid to be tested to vibrate; the deformation measuring unit is used for measuring the deformation amount of the elastic element when the sample cup vibrates; the controller is used for calculating and obtaining the elastic force value of the liquid to be measured according to the deformation amount. The elasticity chart instrument does not need to carry out level adjustment treatment on the instrument before testing, is simpler and more convenient to operate, can effectively reduce the influence of vibration interference, and further improves the stability and accuracy of test data.
Embodiment two:
the present embodiment provides another elastograph, which is implemented on the basis of the elastograph shown in fig. 1.
In this embodiment, the elastography apparatus includes a coupling reed, an elastic element, a deformation measuring unit, a sample cup vibration unit, and a controller, wherein the controller is connected to the deformation measuring unit, one end of the coupling reed is connected to the elastic element, and the other end of the coupling reed is immersed in the liquid to be measured. On the basis, the elastography instrument further comprises a sample adding device; the sample adding device comprises a sample adding needle and a mechanical arm unit.
The mechanical arm unit is connected with the controller, and the sample adding needle is arranged on the mechanical arm unit. In actual operation, the controller controls the mechanical arm unit to correspondingly operate so as to move the sample adding needle to the position of the sample cup, and the liquid to be detected is added into the sample cup through the sample adding needle, so that automation of sample adding operation is realized.
In some cases, the elastic force of the liquid to be tested is affected by the temperature of the liquid, and in order to measure a more stable elastic force, the temperature of the liquid to be tested needs to be kept relatively stable. In one possible embodiment, an incubation device may be added to the elastography apparatus, wherein the incubation device comprises a first cuvette holder and a heating unit.
Here, the first cuvette placement groove is provided on the above-mentioned cuvette vibration unit, and the first cuvette placement groove is used for placing a cuvette containing a liquid to be measured. That is, a first cuvette placement groove is further provided on the cuvette vibration unit, and a sample which has been subjected to sample addition is prepared to be placed in the first cuvette placement groove to be tested. And the heating unit is used for heating the sample cup so as to maintain the constant temperature of the liquid to be measured. In this way, in the process of testing the liquid to be tested, the liquid to be tested always keeps constant temperature, so that the stability of test data is improved.
In order to improve the testing efficiency, in one possible implementation manner, a sample cup supply device and a sample cup conveying device can be further arranged in the elastography instrument so as to realize automatic acquisition and automatic conveying of the sample cups. Wherein, this appearance cup supply device includes: the second sample cup placing groove and the cup feeding unit, wherein the second sample cup placing groove is used for placing an empty sample cup; the cup feeding unit is used for acquiring an empty sample cup from the second sample cup placing groove and placing the empty sample cup at a first preset position. And, this sample cup conveyer includes: the sample cup grabbing unit and the conveying guide rail, wherein the sample cup grabbing unit is movably arranged on the conveying guide rail. The sample cup grabbing unit is used for grabbing a sample cup from a first preset position; the conveying guide rail is used for conveying the sample cups grabbed by the sample cup grabbing unit to a second preset position.
In another possible embodiment, the liquid to be tested is a mixture of a blood sample and a coagulation reagent. And, this elastography appearance still includes: sample and reagent supply means. Wherein the sample and reagent supply means comprises: test tube tray, rotary unit and sweep a yard unit. The sample test tube and the reagent test tube are placed on the test tube storage disc, the blood sample is placed in the sample test tube, the blood coagulation reagent is placed in the reagent test tube, the corresponding identification codes are arranged on the sample test tube and the reagent test tube, here, the identification codes and the test tubes are in one-to-one correspondence, each test tube corresponds to a unique identification code, and different test tubes and samples or reagents therein can be distinguished according to the identification codes.
In addition, the code scanning unit is arranged on one side of the test tube storage disc. The rotating unit is used for driving the test tube storage disc to do rotating motion so as to rotate different test tubes into the scanning range of the code scanning unit. And the code scanning unit is used for scanning and reading the identification code in the scanning range so as to acquire the information of the current test tube. That is, when a sample or reagent is required, the code scanning unit reads the identification code on the test tube within the scanning range by rotating the test tube storage tray, thereby identifying the current information of the test tube including the type of the liquid in the test tube until the required sample or reagent is found, so that it can be provided to the sample adding device to add the required sample or reagent to the sample cup.
According to the elastography instrument provided by the embodiment, through the arrangement of the sample cup supply device, the sample cup conveying device, the sample and reagent supply device, the sample adding device, the incubation device and other mechanical control structures, the whole process of sample cup acquisition, sample addition, sample cup conveying and sample incubation to test is automated, personnel operation is not needed, the degree of automation of elastography test is improved, the influence of human factors is reduced, and further the stability and accuracy of test data are improved.
Embodiment III:
the present embodiment provides another elastography apparatus for detecting thrombus, referring to fig. 2, which is a schematic structural diagram of the elastography apparatus, and as can be seen from fig. 2, the elastography apparatus includes: a cuvette supply unit 21, a cuvette transfer unit 22, a sample and reagent supply unit 23, a loading unit 24, an incubation unit 25 and a measurement unit 26.
In this embodiment, the cuvette supply means 21 comprises base means for holding a cuvette, positioning means for the cuvette and cuvette feeding means for the cuvette. The sample cup base is used for containing a plurality of sample cups, and the limiting structure of the sample cups is fixed on the sample cup base so as to ensure that the sample cups have relative fixed positions in the moving process. In addition, the cup feeding device of the sample cup is used for ensuring that the sample cup placed on the sample cup base can move up and down or horizontally to a designated position so that the movable arm assembly can grasp the sample cup.
Furthermore, the cuvette conveyor 22 comprises a rail base, a moving rail in the transverse and longitudinal directions, a drive motor and a gripping cuvette mechanism. The guide rail base is used for fixing the moving guide rail; the transverse and longitudinal movement guide rails are used for ensuring that the position of the reaction cup grabbing mechanism is accurate and stable when the reaction cup is grabbed. In addition, the drive motor provides power for the grabbing reaction cup mechanism. The reaction cup grabbing mechanism conveys the reaction cup from the sample cup containing base device to a sample adding position, and is responsible for conveying the sample cup to the sample cup placing groove for detection after the sample and the reagent are added.
For this sample and reagent supply means 23, it comprises a sample and reagent disk, a rotation mechanism and a code scanning mechanism. Furthermore, the sample and the reagent disk are circumferentially distributed, and a plurality of grooves are formed on the sample and the reagent disk for placing the sample tube and the reagent tube. Wherein, rotary mechanism provides power in order to drive sample and reagent dish and do circular motion. The code scanning mechanism is responsible for scanning the identification codes on the code sample and the reagent to distinguish different samples and reagents. In this embodiment, the sample is a blood sample, and the reagent is a clotting reagent for accelerating clotting of blood.
Furthermore, the loading device 24 includes a loading needle and a rotating mechanical arm. And the sample adding needle is responsible for adding samples and reagents into the sample cup according to a certain quantity, and the rotary mechanical arm has a horizontal rotation function and can vertically move so as to ensure that the reagents and the samples are accurately added into the sample cup.
In addition, the incubation means 25 comprises a cuvette holder, a cuvette heating means and a cuvette driving means. The sample cup placing grooves are transversely arranged, a plurality of reaction cups can be placed at the same time, and the sample cup heating device ensures that the temperature of a sample is constant in the testing process. The sample cup driving device drives the sample cup to do linear motion by the power provided by the electric motor.
Further, the measuring device 26 includes a coupling reed, an elastic element, a deformation measuring unit, a cuvette vibrating unit, and a controller; the controller is connected with the deformation measuring unit; one end of the coupling reed is connected with the elastic element, and the other end of the coupling reed is immersed in the liquid to be measured. The measuring device 26 further comprises a measuring element moving unit connected with the deformation measuring unit, and the measuring element moving unit can provide vertical movement to drive the deformation measuring unit to move up and down, so that the coupling reed can be immersed in or separated from the liquid to be measured.
In actual measurement, after receiving the start measurement signal, the measuring piece moving unit moves downwards to send the coupling reed into the sample cup. The sample cup moves back and forth, because the sample is not solidified yet, the generated viscoelasticity is smaller, the viscous force between the coupling reed and the sample is also smaller in the initial measurement stage, the coupling reed is basically in a static state, along with the solidification start of the sample, the viscous force born by the coupling reed is larger and larger, the forced vibration elastic deformation of the coupling reed is larger, the deformation is recorded by the deformation measurement unit, a group of corresponding relation curves of the viscous force and the deformation quantity of the coupling reed are formed, the change of the amplitude and the change of the elasticity accord with a certain fixed relation, the positive correlation relation is formed, the relation curve of the elasticity and the deformation quantity can be further corresponding, and the elasticity of the liquid to be measured can be measured by continuously collecting the forced amplitude of the elastic element.
In order to verify the performance of the elastography machine in this embodiment, TEG5000 of Haemonetics is used as a calibration source, and the same sample is measured to obtain the corresponding relationship between the invention and the measured parameters of the U.S. TEG5000 machine as shown in the figure3, wherein the abscissa of fig. 3 is a TEG5000 elastic force test value, and the ordinate is elastic force original data measured by the elastograph of the invention, and the two represent a certain linear relationship. Proved by verification, the measured value x of the elastography instrument provided by the invention 0 The positive correlation with the A value measured by TEG5000 can be expressed by the following formula:
x 0 ＝C 1 A+C 2 A 3 。
wherein C is 1 、C 2 Is constant, A is the elasticity test value of TEG5000, x 0 Raw data representing the elasticity measured by an elastograph, typically a voltage value.
Therefore, the elastograph provided by the embodiment of the invention has better test performance, and the test result is reliable.
Compared with a thrombus elasticity measuring device in the prior art, the elasticity force measuring device provided by the embodiment is used for detecting the elasticity of thrombus, the elasticity force measuring device replaces a torsion wire in a conventional elasticity force measuring device with a coupling reed, and calculates the elasticity value of liquid to be measured by measuring the vibration amplitude of the coupling reed. And moreover, through setting up mechanical control structure, make the acquisition of appearance cup, add the sample, convey appearance cup, incubate to the test, whole process realizes the automation, improves the degree of automation of elasticity diagram test, has reduced the influence of human factor, and then improves test data's stability and accuracy.
Embodiment four:
the embodiment of the present invention further provides a visualized elastography device, as shown in fig. 4, which is a schematic structural diagram of the visualized elastography device, and as seen in fig. 4, the visualized elastography device 100 includes the elastography device 41 provided in one of the above-mentioned first embodiment, the second embodiment, the third embodiment and the possible implementation manners thereof, and further includes a display 42. The display 42 is connected to the controller, and the display 42 is used for displaying the elasticity value of the liquid to be measured. Thus, when the elastic force value of the liquid to be measured is measured by the elastic force chart instrument 41, the elastic force data value can be timely obtained from the display 42, and the liquid to be measured is more convenient.
The implementation principle and the generated technical effects of the visualized elastography device provided by the embodiment of the invention are the same as those of the embodiment of the elastography device, and for the sake of brief description, reference is made to corresponding contents in the embodiment of the elastography device where the embodiment of the visualized elastography device is not mentioned.
The relative steps, numerical expressions and numerical values of the components and steps set forth in these embodiments do not limit the scope of the present invention unless it is specifically stated otherwise.
Any particular values in all examples shown and described herein are to be construed as merely illustrative and not a limitation, and thus other examples of exemplary embodiments may have different values.
In addition, in the description of embodiments of the present invention, unless explicitly stated and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
In the description of the present invention, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
Finally, it should be noted that: the above examples are only specific embodiments of the present invention, and are not intended to limit the scope of the present invention, but it should be understood by those skilled in the art that the present invention is not limited thereto, and that the present invention is described in detail with reference to the foregoing examples: any person skilled in the art may modify or easily conceive of the technical solution described in the foregoing embodiments, or perform equivalent substitution of some of the technical features, while remaining within the technical scope of the present disclosure; such modifications, changes or substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention, and are intended to be included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (7)
1. An elastograph, comprising: the device comprises a coupling reed, an elastic element, a deformation measuring unit, a sample cup vibration unit and a controller; the controller is connected with the deformation measuring unit; one end of the coupling reed is connected with the elastic element, and the other end of the coupling reed is immersed in the liquid to be detected;
the sample cup vibration unit is used for driving the sample cup filled with the liquid to be tested to vibrate;
the deformation measuring unit is used for measuring the deformation amount of the elastic element when the sample cup vibrates;
the controller is used for calculating and obtaining the elastic force value of the liquid to be measured according to the deformation;
the coupling reed is made of phosphor bronze and tin bronze;
the elastic element comprises an elastic rod, an elastic wire or an elastic sheet;
the elastograph further comprises: a sample adding device; the sample adding device comprises a sample adding needle and a mechanical arm unit; the mechanical arm unit is connected with the controller, and the sample adding needle is arranged on the mechanical arm unit;
the mechanical arm unit is used for moving the sample adding needle to the position of the sample cup so as to add the liquid to be tested into the sample cup through the sample adding needle;
the elastograph further comprises: an incubation device; the incubation device comprises a first cup placing groove and a heating unit; the first sample cup placing groove is arranged on the sample cup vibrating unit;
the first sample cup placing groove is used for placing the sample cup containing the liquid to be tested;
the heating unit is used for heating the sample cup so as to maintain the constant temperature of the liquid to be detected.
2. The elastography machine of claim 1, wherein the deformation measurement unit comprises a hall sensor or a photoelectric sensor.
3. The elastograph of claim 1, further comprising: a sample cup supply device; the cuvette supply apparatus includes: a second sample cup placing groove and a cup feeding unit;
the second sample cup placing groove is used for placing an empty sample cup;
the cup feeding unit is used for acquiring the empty sample cup from the second sample cup placing groove and placing the empty sample cup at a first preset position.
4. The elastograph of claim 1, further comprising: a sample cup transfer device; the sample cup transfer apparatus includes: a sample cup gripping unit and a conveying guide rail; the sample cup grabbing unit is movably arranged on the conveying guide rail;
the sample cup grabbing unit is used for grabbing a sample cup from a first preset position;
the conveying guide rail is used for conveying the sample cups grabbed by the sample cup grabbing unit to a second preset position.
5. The elastography apparatus of any of claims 1-4, wherein the fluid to be tested is a mixture of a blood sample and a clotting reagent.
6. The elastograph of claim 5, further comprising: sample and reagent supply means; the sample and reagent supply device includes: test tube storage disc, rotation unit and code scanning unit;
a sample test tube and a reagent test tube are placed on the test tube storage tray, a blood sample is placed in the sample test tube, a blood coagulation reagent is placed in the reagent test tube, and corresponding identification codes are arranged on the sample test tube and the reagent test tube; the code scanning unit is arranged on one side of the test tube storage disc;
the rotating unit is used for driving the test tube storage disc to do rotating motion so as to rotate different test tubes into the scanning range of the code scanning unit;
the code scanning unit is used for scanning and reading the identification codes in the scanning range so as to acquire the information of the current test tube.
7. A visual elastography device, comprising: the elastograph of any of claims 1-6, further comprising a display; the display is connected with the controller;
the display is used for displaying the elastic force value of the liquid to be detected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910623306.5A CN110208511B (en) | 2019-07-11 | 2019-07-11 | Elastography instrument and visualized elastography equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910623306.5A CN110208511B (en) | 2019-07-11 | 2019-07-11 | Elastography instrument and visualized elastography equipment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110208511A CN110208511A (en) | 2019-09-06 |
| CN110208511B true CN110208511B (en) | 2024-03-08 |
Family
ID=67797245
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910623306.5A Active CN110208511B (en) | 2019-07-11 | 2019-07-11 | Elastography instrument and visualized elastography equipment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110208511B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117451979B (en) * | 2023-12-22 | 2024-03-12 | 苏州思迈德生物科技有限公司 | Method for converting sample cup rotation curve into probe rotation curve |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1498645A1 (en) * | 1963-09-27 | 1969-06-04 | Coulter Electronics | Automatic coagulometer |
| CN104458503A (en) * | 2014-12-12 | 2015-03-25 | 广州阳普医疗科技股份有限公司 | Blood coagulation detection instrument |
| CN104981477A (en) * | 2012-08-22 | 2015-10-14 | 生物医学美国有限公司 | Device and method for performing blood thromboelastographic assays by magnetic sensing |
| CN105548583A (en) * | 2016-01-11 | 2016-05-04 | 广州阳普医疗科技股份有限公司 | Blood coagulation detecting instrument |
| CN107315094A (en) * | 2017-07-27 | 2017-11-03 | 深圳传世生物医疗有限公司 | Blood coagulation analyzer and blood coagulation analyzing method |
| CN109975277A (en) * | 2017-12-28 | 2019-07-05 | 深圳市新产业生物医学工程股份有限公司 | Chemiluminescence detector and its detection method |
| CN210465441U (en) * | 2019-07-11 | 2020-05-05 | 北京森美希克玛生物科技有限公司 | Elastogram appearance and visual elastogram equipment |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2444956A (en) * | 2006-12-19 | 2008-06-25 | Pentapharm Ag | An apparatus and method for measuring the coagulation characteristics of a test liquid |
| WO2014100378A1 (en) * | 2012-12-19 | 2014-06-26 | The General Hospital Corporation | Optical blood-coagulation sensor |
-
2019
- 2019-07-11 CN CN201910623306.5A patent/CN110208511B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1498645A1 (en) * | 1963-09-27 | 1969-06-04 | Coulter Electronics | Automatic coagulometer |
| CN104981477A (en) * | 2012-08-22 | 2015-10-14 | 生物医学美国有限公司 | Device and method for performing blood thromboelastographic assays by magnetic sensing |
| CN104458503A (en) * | 2014-12-12 | 2015-03-25 | 广州阳普医疗科技股份有限公司 | Blood coagulation detection instrument |
| CN105548583A (en) * | 2016-01-11 | 2016-05-04 | 广州阳普医疗科技股份有限公司 | Blood coagulation detecting instrument |
| CN107315094A (en) * | 2017-07-27 | 2017-11-03 | 深圳传世生物医疗有限公司 | Blood coagulation analyzer and blood coagulation analyzing method |
| CN109975277A (en) * | 2017-12-28 | 2019-07-05 | 深圳市新产业生物医学工程股份有限公司 | Chemiluminescence detector and its detection method |
| CN210465441U (en) * | 2019-07-11 | 2020-05-05 | 北京森美希克玛生物科技有限公司 | Elastogram appearance and visual elastogram equipment |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110208511A (en) | 2019-09-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US3635678A (en) | Clot-timing system and method | |
| JP4485137B2 (en) | Calibration method of clinical analyzer and automatic alignment method of dispenser of clinical analyzer | |
| US3967934A (en) | Prothrombin timer | |
| JP6831408B2 (en) | Calibration process and system | |
| CN204065105U (en) | A kind of thrombus elasticity testing device | |
| CN208459417U (en) | A kind of full-automatic sample introduction mechanism of flow cytometer | |
| JP2018185160A (en) | Blood specimen analysis method, analyzer and computer program | |
| CN104181311A (en) | Thrombus elasticity testing device | |
| JP4926854B2 (en) | Platelet aggregation measurement method and platelet aggregation measurement apparatus | |
| EP3505937B1 (en) | Blood coagulation test device and blood coagulation test method | |
| GB2444956A (en) | An apparatus and method for measuring the coagulation characteristics of a test liquid | |
| CN107525915B (en) | A kind of thrombelastogram instrument | |
| CN204479595U (en) | A kind of sample cup assembly and there is the Hemostasis examination instrument of this assembly | |
| CN110208511B (en) | Elastography instrument and visualized elastography equipment | |
| JPWO2014097973A1 (en) | Automatic analyzer | |
| CN210465441U (en) | Elastogram appearance and visual elastogram equipment | |
| JP2021518906A (en) | Rotary viscometer for measuring the viscosity of a substance | |
| CN106771108B (en) | Method and device for automatically acquiring thromboelastography | |
| CN101858919B (en) | Automatic analyzer | |
| CN113015906B (en) | Coagulation analyzer and fibrinogen concentration detection method thereof | |
| CN217954445U (en) | Detection probe for thromboelastogram | |
| CN115144574A (en) | Detection device for thrombelastogram | |
| CN115144575A (en) | Detection probe for thromboelastogram | |
| US11420197B2 (en) | Apparatus and method for mixing fluid or media by vibrating a pipette using nonconcentric masses | |
| CN217954444U (en) | Detection device for thrombelastogram |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |